An shRNA-Based Screen of Splicing Regulators Identifies SFRS3 as a Negative Regulator of IL-1β Secretion
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"An shRNA-based screen of splicing regulators identifies SFRS3 as a negative regulator of IL-1β secretion", "type"=>"journal", "authors"=>[{"first_name"=>"Pedro", "last_name"=>"Moura-Alves", "scopus_author_id"=>"36677779100"}, {"first_name"=>"Ana", "last_name"=>"Neves-Costa", "scopus_author_id"=>"15124043500"}, {"first_name"=>"Helena", "last_name"=>"Raquel", "scopus_author_id"=>"35788497300"}, {"first_name"=>"Teresa Raquel", "last_name"=>"Pacheco", "scopus_author_id"=>"55953393400"}, {"first_name"=>"Bruno", "last_name"=>"D'Almeida", "scopus_author_id"=>"8560687800"}, {"first_name"=>"Raquel", "last_name"=>"Rodrigues", "scopus_author_id"=>"39862203700"}, {"first_name"=>"Iris", "last_name"=>"Cadima-Couto", "scopus_author_id"=>"33567520100"}, {"first_name"=>"Ângelo", "last_name"=>"Chora", "scopus_author_id"=>"18834528400"}, {"first_name"=>"Mariana", "last_name"=>"Oliveira", "scopus_author_id"=>"57199779370"}, {"first_name"=>"Margarida", "last_name"=>"Gama-Carvalho", "scopus_author_id"=>"6602645433"}, {"first_name"=>"Nir", "last_name"=>"Hacohen", "scopus_author_id"=>"6603121220"}, {"first_name"=>"Luis F.", "last_name"=>"Moita", "scopus_author_id"=>"8638960300"}], "year"=>2011, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-79956068797", "sgr"=>"79956068797", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0019829", "pmid"=>"21611201", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pui"=>"361783763"}, "id"=>"fd1b8df1-fcc1-3e0c-9e42-be9df7ac7a41", "abstract"=>"The generation of diversity and plasticity of transcriptional programs are key components of effective vertebrate immune responses. The role of Alternative Splicing has been recognized, but it is underappreciated and poorly understood as a critical mechanism for the regulation and fine-tuning of physiological immune responses. Here we report the generation of loss-of-function phenotypes for a large collection of genes known or predicted to be involved in the splicing reaction and the identification of 19 novel regulators of IL-1β secretion in response to E. coli challenge of THP-1 cells. Twelve of these genes are required for IL-1β secretion, while seven are negative regulators of this process. Silencing of SFRS3 increased IL-1β secretion due to elevation of IL-1β and caspase-1 mRNA in addition to active caspase-1 levels. This study points to the relevance of splicing in the regulation of auto-inflammatory diseases.", "link"=>"http://www.mendeley.com/research/shrnabased-screen-splicing-regulators-identifies-sfrs3-negative-regulator-il1%CE%B2-secretion", "reader_count"=>29, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>4, "Researcher"=>10, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>9, "Student > Master"=>3}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>4, "Researcher"=>10, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>9, "Student > Master"=>3}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>20, "Medicine and Dentistry"=>4, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>20}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"Sweden"=>1, "Germany"=>1}, "group_count"=>1}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/79956068797"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/79956068797?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79956068797&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=79956068797&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/79956068797", "dc:identifier"=>"SCOPUS_ID:79956068797", "eid"=>"2-s2.0-79956068797", "dc:title"=>"An shRNA-based screen of splicing regulators identifies SFRS3 as a negative regulator of IL-1β secretion", "dc:creator"=>"Moura-Alves P.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"6", "prism:issueIdentifier"=>"5", "prism:pageRange"=>nil, "prism:coverDate"=>"2011-05-23", "prism:coverDisplayDate"=>"2011", "prism:doi"=>"10.1371/journal.pone.0019829", "citedby-count"=>"7", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"University of Lisbon Faculty of Medicine, Institute of Molecular Medicine", "affiliation-city"=>"Lisbon", "affiliation-country"=>"Portugal"}], "pubmed-id"=>"21611201", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e19829", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0019829", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"5", "year"=>"2011", "pdf_views"=>"49", "xml_views"=>"8", "html_views"=>"286"}
  • {"month"=>"6", "year"=>"2011", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"63"}
  • {"month"=>"7", "year"=>"2011", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"8", "year"=>"2011", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"9", "year"=>"2011", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"10", "year"=>"2011", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"11", "year"=>"2011", "pdf_views"=>"14", "xml_views"=>"4", "html_views"=>"36"}
  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"72"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"6", "xml_views"=>"4", "html_views"=>"36"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"51"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"40"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"41"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"14", "html_views"=>"40"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"52"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"22"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"39"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"53"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"70"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"2", "html_views"=>"11"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"3", "html_views"=>"2"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"1"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"2"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/774671"], "description"=>"<p>(A) Comparison of the kinetics of IL-1β mRNA induction after <i>E. coli</i> challenge in SFRS3 depleted cells and control interfered THP-1 cells; (B) Comparison of the kinetics of IL-8 mRNA induction after <i>E. coli</i> challenge in SFRS3 depleted cells and control interfered THP-1 cells; (C) the same for caspase-1 mRNA; and (D) measurement of active caspase-1 by FACS using fluorescent caspase-1 substrate (FLICA); (E) Kinetics of IL-1β mRNA induction after <i>E. coli</i> challenge in HMGB1 depleted THP-1 cells.</p>", "links"=>[], "tags"=>["depletion", "increases", "mrna", "caspase-1"], "article_id"=>445034, "categories"=>["Biochemistry", "Genetics", "Immunology"], "users"=>["Pedro Moura-Alves", "Ana Neves-Costa", "Helena Raquel", "Teresa Raquel Pacheco", "Bruno D'Almeida", "Raquel Rodrigues", "Iris Cadima-Couto", "Ângelo Chora", "Mariana Oliveira", "Margarida Gama-Carvalho", "Nir Hacohen", "Luis F. Moita"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0019829.g004", "stats"=>{"downloads"=>3, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_SFRS3_depletion_increases_IL_1_946_mRNA_and_active_caspase_1_levels_/445034", "title"=>"SFRS3 depletion increases IL-1β mRNA and active caspase-1 levels.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-05-17 01:23:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/774840"], "description"=>"<p>IL-1β secretion candidate negative regulator genes.</p>", "links"=>[], "tags"=>["secretion"], "article_id"=>445198, "categories"=>["Biochemistry", "Genetics", "Immunology"], "users"=>["Pedro Moura-Alves", "Ana Neves-Costa", "Helena Raquel", "Teresa Raquel Pacheco", "Bruno D'Almeida", "Raquel Rodrigues", "Iris Cadima-Couto", "Ângelo Chora", "Mariana Oliveira", "Margarida Gama-Carvalho", "Nir Hacohen", "Luis F. Moita"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0019829.t002", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_IL_1_946_secretion_candidate_negative_regulator_genes_/445198", "title"=>"IL-1β secretion candidate negative regulator genes.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2011-05-17 01:26:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/774587"], "description"=>"<p>(A) Kinetics of IL-1β secretion in HMGB1 KD cells after <i>E. coli</i> stimulation in comparison to the shScram control; and (B) validation of HMGB1 KD by immunoblotting; (C) SFRS3 protein left after SFRS3 silencing in THP-1 cells is plotted against the phenotype for each construct as measured by IL-1β secretion, construct #2 is not shown because most cells died; (D) Secretion of IL-1β in SFRS3 KD cells after <i>E. coli</i> stimulation in comparison to the shScram control; and (E) validation of SFRS3 KD by immunoblotting; (F) SFRS3 mRNA left after SFRS3 silencing in mouse bone marrow-derived dendritic cells is plotted against the phenotype for each construct as measured by IL-1β secretion, constructs #1 and #2 are not shown because most cells died; (G) IL-1β secretion after <i>E. coli</i> stimulation in THP-1 cells over-expressing SFRS3; and (H) validation of SFRS3 over-expression by immunoblotting using a specific antibody against the V5 tag; (I) IL-1β secretion by THP-1 cells after 24 hrs of <i>E. coli</i> challenge in the presence of growing concentrations of the SFRS3 depleting drug digoxin; (J) validation of SFRS3 depletion by immunoblotting using a specific antibody against SFRS3. SFRS3 levels are down modulated in monocytes in response to an <i>E. coli</i> challenge. (L) Kinetics of SFRS3 mRNA expression in monocytes purified from Human PBMCs after <i>E. coli</i> stimulation; (M) SFRS3 protein levels in the same conditions, by immunoblotting using a specific antibody against SFRS3.</p>", "links"=>[], "tags"=>["sfrs3"], "article_id"=>444947, "categories"=>["Biochemistry", "Genetics", "Immunology"], "users"=>["Pedro Moura-Alves", "Ana Neves-Costa", "Helena Raquel", "Teresa Raquel Pacheco", "Bruno D'Almeida", "Raquel Rodrigues", "Iris Cadima-Couto", "Ângelo Chora", "Mariana Oliveira", "Margarida Gama-Carvalho", "Nir Hacohen", "Luis F. Moita"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0019829.g003", "stats"=>{"downloads"=>2, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Validation_of_SFRS3_as_a_novel_regulator_of_IL_1_946_secretion_/444947", "title"=>"Validation of SFRS3 as a novel regulator of IL-1β secretion.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-05-17 01:22:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/388958"], "description"=>"<div><p>The generation of diversity and plasticity of transcriptional programs are key components of effective vertebrate immune responses. The role of Alternative Splicing has been recognized, but it is underappreciated and poorly understood as a critical mechanism for the regulation and fine-tuning of physiological immune responses. Here we report the generation of loss-of-function phenotypes for a large collection of genes known or predicted to be involved in the splicing reaction and the identification of 19 novel regulators of IL-1β secretion in response to <em>E. coli</em> challenge of THP-1 cells. Twelve of these genes are required for IL-1β secretion, while seven are negative regulators of this process. Silencing of SFRS3 increased IL-1β secretion due to elevation of IL-1β and caspase-1 mRNA in addition to active caspase-1 levels. This study points to the relevance of splicing in the regulation of auto-inflammatory diseases.</p> </div>", "links"=>[], "tags"=>["shrna-based", "splicing", "regulators", "identifies", "sfrs3", "secretion"], "article_id"=>136788, "categories"=>["Biochemistry", "Genetics", "Immunology"], "users"=>["Pedro Moura-Alves", "Ana Neves-Costa", "Helena Raquel", "Teresa Raquel Pacheco", "Bruno D'Almeida", "Raquel Rodrigues", "Iris Cadima-Couto", "Ângelo Chora", "Mariana Oliveira", "Margarida Gama-Carvalho", "Nir Hacohen", "Luis F. Moita"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0019829", "stats"=>{"downloads"=>4, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/An_shRNA_Based_Screen_of_Splicing_Regulators_Identifies_SFRS3_as_a_Negative_Regulator_of_IL_1_Secretion/136788", "title"=>"An shRNA-Based Screen of Splicing Regulators Identifies SFRS3 as a Negative Regulator of IL-1β Secretion", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2011-05-17 01:53:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/774760"], "description"=>"<p>(A) IL-1β mRNA as determined using exonic or intronic primers after 8 hours of <i>E. coli</i> challenge. One representative experiment is shown; (B) Plot begins after 2 hours of <i>E. coli</i> challenge. Time on the x-axis indicates the period of Actinomycin D treatment. One representative experiment is shown. On the right, phenotypic control for this experiment as measured by IL-1β secret ion is shown (triplicates with standard deviations are shown for three independent measurements); (C, D, E) three independent experiments of NF-κB reporter activation on control or SFRS3 shRNA treated cells as measured by FACS and expressed in Mean Fluorescence Intensity (MFI). For each figure/experiment, triplicates with standard deviations are shown for three independent measurements and one representative measure for each sample is plotted bellow.</p>", "links"=>[], "tags"=>["regulates", "transcription", "unchanged", "nf-"], "article_id"=>445110, "categories"=>["Biochemistry", "Genetics", "Immunology"], "users"=>["Pedro Moura-Alves", "Ana Neves-Costa", "Helena Raquel", "Teresa Raquel Pacheco", "Bruno D'Almeida", "Raquel Rodrigues", "Iris Cadima-Couto", "Ângelo Chora", "Mariana Oliveira", "Margarida Gama-Carvalho", "Nir Hacohen", "Luis F. Moita"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0019829.g005", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_SFRS3_regulates_IL_1_946_transcription_in_the_context_of_unchanged_NF_954_B_reporter_activity_/445110", "title"=>"SFRS3 regulates IL-1β transcription in the context of unchanged NF- κB reporter activity.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-05-17 01:25:10"}
  • {"files"=>["https://ndownloader.figshare.com/files/774882"], "description"=>"<p>IL-1β secretion candidate positive regulator genes.</p>", "links"=>[], "tags"=>["secretion"], "article_id"=>445241, "categories"=>["Biochemistry", "Genetics", "Immunology"], "users"=>["Pedro Moura-Alves", "Ana Neves-Costa", "Helena Raquel", "Teresa Raquel Pacheco", "Bruno D'Almeida", "Raquel Rodrigues", "Iris Cadima-Couto", "Ângelo Chora", "Mariana Oliveira", "Margarida Gama-Carvalho", "Nir Hacohen", "Luis F. Moita"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0019829.t001", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_IL_1_946_secretion_candidate_positive_regulator_genes_/445241", "title"=>"IL-1β secretion candidate positive regulator genes.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2011-05-17 01:27:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/774435"], "description"=>"<p>(A) THP-1 cells were challenged with PFA-fixed <i>E. coli</i> at a 20 bacteria: 1 cell ratio. IL-1β mRNA expression was quantified by qRT-PCR; (B) Caspase-1 activity as measured by FACS using fluorescent caspase-1 substrate (FLICA); (C) In the same conditions, cell conditioned media was collected and the concentration of IL-1β measured by ELISA; (D) Caspase-1 dependent IL-1β secretion at 24 hrs after <i>E. coli</i> challenge is shown by using caspase drug inhibitors; (E) and by measuring IL-1β secretion from cells where caspase-1 was depleted by RNAi; (F) validation of caspase-1 silencing using immunoblotting; (G) Effect of NLRP3 and ASC in IL-1β secretion after 24 hrs of <i>E. coli</i> challenge; (H) Validation of NLRP3 and ASC knockdowns, by immunoblotting using specific antibodies against these inflammasome components; (I) the secretion of IL-1β was compared in THP-1 cells that were stimulated with PFA-fixed <i>E. coli</i> with cells that in addition to <i>E. coli</i> stimulation were treated with either ATP or the P2X7 inhibitor, KN-62.</p>", "links"=>[], "tags"=>["cells", "induce", "secrete"], "article_id"=>444788, "categories"=>["Biochemistry", "Genetics", "Immunology"], "users"=>["Pedro Moura-Alves", "Ana Neves-Costa", "Helena Raquel", "Teresa Raquel Pacheco", "Bruno D'Almeida", "Raquel Rodrigues", "Iris Cadima-Couto", "Ângelo Chora", "Mariana Oliveira", "Margarida Gama-Carvalho", "Nir Hacohen", "Luis F. Moita"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0019829.g001", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_THP_1_cells_induce_and_secrete_IL_1_in_response_to_E_coli_stimulation_/444788", "title"=>"THP-1 cells induce and secrete IL-1β in response to <i>E. coli</i> stimulation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-05-17 01:19:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/774508"], "description"=>"<p>(A) THP-1 cells were infected with shRNA expressing lentiviruses in a 96-well plate format. After puromycin selection, the number of cells was measured and stimulated with PFA-fixed <i>E. coli</i>. Values of secreted IL-1β were normalized by dividing the amount of IL-1β in the conditioned media 24 hrs after <i>E. coli</i> stimulation by the number of cells in each well and then by the average concentration per cell of the plate. Results were logarithmic natural transformed. Scores were sorted in ascending order and graphed; (B) Phenotypes for 12 positive regulators and 7 negative regulators of IL-1β secretion are shown in comparison to shScram control; (C) Validation of candidates by measurement of mRNA levels after silencing using qRT-PCR.</p>", "links"=>[], "tags"=>["validation", "splicing", "factors"], "article_id"=>444861, "categories"=>["Biochemistry", "Genetics", "Immunology"], "users"=>["Pedro Moura-Alves", "Ana Neves-Costa", "Helena Raquel", "Teresa Raquel Pacheco", "Bruno D'Almeida", "Raquel Rodrigues", "Iris Cadima-Couto", "Ângelo Chora", "Mariana Oliveira", "Margarida Gama-Carvalho", "Nir Hacohen", "Luis F. Moita"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0019829.g002", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Identification_and_validation_of_novel_splicing_factors_with_a_role_in_IL_1_946_secretion_/444861", "title"=>"Identification and validation of novel splicing factors with a role in IL-1β secretion.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-05-17 01:21:01"}

PMC Usage Stats | Further Information

  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"16", "unique-ip"=>"15", "pdf"=>"8", "year"=>"2011", "figure"=>"9", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"29", "year"=>"2011", "pdf"=>"22", "unique-ip"=>"30", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"19", "unique-ip"=>"18", "pdf"=>"2", "year"=>"2011", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"17", "year"=>"2011", "pdf"=>"5", "unique-ip"=>"16", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"17", "unique-ip"=>"21", "pdf"=>"9", "year"=>"2011", "figure"=>"19", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"21", "year"=>"2011", "pdf"=>"8", "unique-ip"=>"23", "figure"=>"12", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"13", "unique-ip"=>"12", "pdf"=>"7", "year"=>"2011", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"15", "year"=>"2011", "pdf"=>"5", "unique-ip"=>"16", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"10", "unique-ip"=>"17", "pdf"=>"6", "year"=>"2012", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"13", "year"=>"2012", "pdf"=>"8", "unique-ip"=>"11", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"20", "unique-ip"=>"19", "pdf"=>"10", "year"=>"2012", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"16", "year"=>"2012", "pdf"=>"5", "unique-ip"=>"13", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"15", "unique-ip"=>"11", "pdf"=>"5", "year"=>"2012", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"7", "year"=>"2012", "pdf"=>"3", "unique-ip"=>"7", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"7", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"14", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"17", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"1", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"1", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"14", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"14", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"2", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"5", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"1", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"2", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}

Relative Metric

{"start_date"=>"2011-01-01T00:00:00Z", "end_date"=>"2011-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Physiology", "average_usage"=>[296, 551, 687, 800, 903, 1001, 1095, 1184, 1259, 1327, 1406, 1473, 1535, 1600, 1669, 1736, 1794, 1857, 1922, 1983, 2046, 2109, 2169, 2223, 2278, 2344, 2402, 2457, 2517, 2574, 2621, 2688, 2756, 2811, 2872, 2936, 2985]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[303, 571, 709, 823, 937, 1038, 1133, 1216, 1298, 1370, 1447, 1518, 1588, 1656, 1725, 1790, 1856, 1926, 1992, 2054, 2118, 2182, 2242, 2301, 2363, 2425, 2484, 2544, 2602, 2662, 2716, 2776, 2841, 2901, 2960, 3016, 3071]}, {"subject_area"=>"/Medicine and health sciences/Immunology", "average_usage"=>[299, 582, 725, 844, 971, 1076, 1167, 1253, 1328, 1403, 1480, 1551, 1620, 1691, 1756, 1821, 1893, 1962, 2032, 2110, 2178, 2236, 2300, 2358, 2419, 2465, 2529, 2587, 2643, 2700, 2756, 2817, 2869, 2932, 2986, 3046, 3101]}, {"subject_area"=>"/Medicine and health sciences/Physiology", "average_usage"=>[291, 540, 674, 787, 893, 986, 1087, 1172, 1249, 1319, 1394, 1465, 1526, 1588, 1645, 1713, 1774, 1837, 1903, 1959, 2027, 2085, 2152, 2205, 2259, 2318, 2377, 2436, 2486, 2539, 2603, 2664, 2719, 2779, 2835, 2888, 2944]}]}
Loading … Spinner
There are currently no alerts