NFATc1 Regulation of TRAIL Expression in Human Intestinal Cells
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Nfatc1 regulation of trail expression in human intestinal cells", "type"=>"journal", "authors"=>[{"first_name"=>"Qingding", "last_name"=>"Wang", "scopus_author_id"=>"57199483801"}, {"first_name"=>"Yuning", "last_name"=>"Zhou", "scopus_author_id"=>"9841085600"}, {"first_name"=>"Heidi L.", "last_name"=>"Weiss", "scopus_author_id"=>"7403113158"}, {"first_name"=>"Chi Wing", "last_name"=>"Chow", "scopus_author_id"=>"35744620900"}, {"first_name"=>"B. Mark", "last_name"=>"Evers", "scopus_author_id"=>"7103239079"}], "year"=>2011, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "pui"=>"361783711", "sgr"=>"79956114443", "doi"=>"10.1371/journal.pone.0019882", "scopus"=>"2-s2.0-79956114443", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pmid"=>"21603612"}, "id"=>"a8ad8cae-3c49-3fd8-ad0c-9b973b4eb94b", "abstract"=>"TNF-related apoptosis-inducing ligand (TRAIL; Apo2) has been shown to promote intestinal cell differentiation. Nuclear factor of activated T cells (NFAT) participates in the regulation of a variety of cellular processes, including differentiation. Here, we examined the role of NFAT in the regulation of TRAIL in human intestinal cells. Treatment with a combination of phorbol 12-myristate 13-acetate (PMA) plus the calcium ionophore A23187 (Io) increased NFAT activation and TRAIL expression; pretreatment with the calcineurin inhibitor cyclosporine A (CsA), an antagonist of NFAT signaling, diminished NFAT activation and TRAIL induction. In addition, knockdown of NFATc1, NFATc2, NFATc3, and NFATc4 blocked PMA/Io increased TRAIL protein expression. Expression of NFATc1 activated TRAIL promoter activity and increased TRAIL mRNA and protein expression. Deletion of NFAT binding sites from the TRAIL promoter did not significantly abrogate NFATc1-increased TRAIL promoter activity, suggesting an indirect regulation of TRAIL expression by NFAT activation. Knockdown of NFATc1 increased Sp1 transcription factor binding to the TRAIL promoter and, importantly, inhibition of Sp1, by chemical inhibition or RNA interference, increased TRAIL expression. These studies identify a novel mechanism for TRAIL regulation by which activation of NFATc1 increases TRAIL expression through negative regulation of Sp1 binding to the TRAIL promoter.", "link"=>"http://www.mendeley.com/research/nfatc1-regulation-trail-expression-human-intestinal-cells", "reader_count"=>16, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Student > Doctoral Student"=>1, "Researcher"=>2, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>3, "Student > Master"=>2, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Student > Doctoral Student"=>1, "Researcher"=>2, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>3, "Student > Master"=>2, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>3, "Agricultural and Biological Sciences"=>5, "Medicine and Dentistry"=>6, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>6}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>5}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}}, "reader_count_by_country"=>{"Germany"=>1}, "group_count"=>2}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/79956114443"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/79956114443?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79956114443&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=79956114443&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/79956114443", "dc:identifier"=>"SCOPUS_ID:79956114443", "eid"=>"2-s2.0-79956114443", "dc:title"=>"Nfatc1 regulation of trail expression in human intestinal cells", "dc:creator"=>"Wang Q.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"6", "prism:issueIdentifier"=>"5", "prism:pageRange"=>nil, "prism:coverDate"=>"2011-05-23", "prism:coverDisplayDate"=>"2011", "prism:doi"=>"10.1371/journal.pone.0019882", "citedby-count"=>"16", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"University of Kentucky HealthCare", "affiliation-city"=>"Lexington", "affiliation-country"=>"United States"}, {"@_fa"=>"true", "affilname"=>"University of Kentucky", "affiliation-city"=>"Lexington", "affiliation-country"=>"United States"}], "pubmed-id"=>"21603612", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e19882", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0019882", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"5", "year"=>"2011", "pdf_views"=>"29", "xml_views"=>"10", "html_views"=>"129"}
  • {"month"=>"6", "year"=>"2011", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"7", "year"=>"2011", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"8", "year"=>"2011", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"9", "year"=>"2011", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"10", "year"=>"2011", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"11", "year"=>"2011", "pdf_views"=>"20", "xml_views"=>"5", "html_views"=>"39"}
  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"18", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"6", "xml_views"=>"4", "html_views"=>"21"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"61"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"52"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"4", "html_views"=>"44"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"38"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"20"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"33"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"30"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"69"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"64"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"71"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"28"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"3", "html_views"=>"30"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"19"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"3", "html_views"=>"5"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"4", "html_views"=>"14"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"3"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/774706"], "description"=>"<p>(<b>A</b>) HT29 cells were pre-treated with cyclosporin A (CsA) for 30 min followed by the combination of PMA (100 nM) plus Io (2.5 µM) with CsA for 2 h. Total protein was extracted from cells and resolved by SDS-PAGE, transferred to a PVDF membrane and probed with anti-TRAIL and anti-β-actin antibodies. TRAIL signals from three separate experiments were quantitated densitometrically and expressed as fold-change with respect to β-actin. (Data shown as mean ± standard error of the mean; *, <i>P</i><0.05, PMA/Io or PMA/Io plus CSA vs. control; <sup>+</sup>, <i>P</i><0.05, PMA/Io plus CSA vs. PMA/Io). (<b>B</b>) Cells were treated with PMA/Io for 60 min in the presence or absence of CsA. Nuclear protein was extracted and NFAT DNA binding was analyzed by EMSA. Unlabeled NFAT oligonucleotide was added in molar excess to confirm binding specificity (<i>competitor</i>).</p>", "links"=>[], "tags"=>["induction", "was", "attenuated", "cyclosporin", "potent", "calcineurin", "inhibitor", "ht29"], "article_id"=>445061, "categories"=>["Physiology", "Chemistry", "Genetics", "Developmental Biology"], "users"=>["Qingding Wang", "Yuning Zhou", "Heidi L. Weiss", "Chi-Wing Chow", "B. Mark Evers"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0019882.g001", "stats"=>{"downloads"=>2, "page_views"=>65, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_PMA_Io_mediated_induction_of_TRAIL_expression_was_attenuated_by_cyclosporin_A_a_potent_calcineurin_inhibitor_in_HT29_cells_/445061", "title"=>"PMA/Io-mediated induction of TRAIL expression was attenuated by cyclosporin A, a potent calcineurin inhibitor in HT29 cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 18:23:28"}
  • {"files"=>["https://ndownloader.figshare.com/files/774946"], "description"=>"<p>HT29 cells were transfected with either the control plasmid (pDF30) or Flag-tagged NFATc1. At 48 h posttransfection, cells were harvested. (<b>A</b>) Expression of TRAIL and NFATc1 protein was detected by Western blot using anti-TRAIL or anti-Flag antibodies. Membranes were reprobed with anti-β-actin antibody to assess loading. (<b>B and C</b>) Total RNA was extracted, and RT-PCR (<b>B</b>) or real time PCR (<b>C</b>) performed. (Data shown as mean ± standard error of the mean. *, <i>P</i><0.05, vs. control plasmids). (<b>D</b>) Caco-2 cells were transfected with a proximal 1371 bp TRAIL promoter construct together with either control plasmid pDF30, pCDNA3.1 or Flag-tagged NFATc1, NFATc2, NFATc3 or NFATc4. At 48 h posttransfection, cells were harvested; luciferase activity was then assayed. All results were normalized for transfection efficiency using the pRL-Tk-luc plasmid (Promega). (Data shown as mean ± standard error of the mean. *, <i>P</i><0.05, vs. control plasmids). (<b>E</b>) Expression of NFATc1, NFATc2, NFATc3 and NFATc4 in Caco-2 cells was confirmed by Western blot using anti-Flag antibody. (<b>F</b>) Caco-2 cells were transfected with control plasmid, pDF30, or Flag-tagged NFATc1. At 48 h posttransfection, total RNA was extracted, and RT-PCR performed using primers to human TRAIL and β-actin. TRAIL signals from three separate experiments were quantitated densitometrically and expressed as fold-change with respect to β-actin (Data shown as mean ± standard error of the mean).</p>", "links"=>[], "tags"=>["nfatc1", "ht29", "caco-2"], "article_id"=>445313, "categories"=>["Physiology", "Chemistry", "Genetics", "Developmental Biology"], "users"=>["Qingding Wang", "Yuning Zhou", "Heidi L. Weiss", "Chi-Wing Chow", "B. Mark Evers"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0019882.g003", "stats"=>{"downloads"=>2, "page_views"=>30, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Overexpression_of_NFATc1_increased_TRAIL_expression_in_HT29_and_Caco_2_cells_/445313", "title"=>"Overexpression of NFATc1 increased TRAIL expression in HT29 and Caco-2 cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 18:24:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/774817"], "description"=>"<p>(<b>A</b>) HT29 cells were treated with PMA/Io for 60 min and NFAT DNA binding activity was assessed by EMSA using nuclear extracts. In addition, nuclear extracts were incubated with <sup>32</sup>P-labeled NFAT specific DNA probe alone or in the presence of unlabeled wild type (<i>cold</i>) NFAT oligonucleotide or mutant NFAT oligonucleotide or specific antibodies to either NFATc1, NFATc2, NFATc3, or NFATc4. (<b>B</b>) HT29 cells were transfected with NFATc1, c2, c3, c4 or control siRNA. After a 48 h incubation, transfected cells were treated with PMA/Io for 2 h. Total protein was extracted and TRAIL expression levels were determined by Western blotting using anti-TRAIL antibody. The membrane was stripped and reprobed using anti-β-actin antibody to confirm equal loading. TRAIL signals from three separate experiments were quantitated densitometrically and expressed as fold-change with respect to β-actin. (Data shown as mean ± standard error of the mean; *, <i>P</i><0.05, PMA/Io plus control siRNA vs. control siRNA; <sup>+</sup>, <i>P</i><0.05, PMA/Io plus NFATC1 siRNA or NFATC4 siRNA vs. PMA/Io plus control siRNA). (<b>C</b>) To confirm NFAT suppression, total RNA was extracted from cells and NFAT expression was assessed by RT-PCR.</p>", "links"=>[], "tags"=>["nfat", "blocked", "ht29"], "article_id"=>445189, "categories"=>["Physiology", "Chemistry", "Genetics", "Developmental Biology"], "users"=>["Qingding Wang", "Yuning Zhou", "Heidi L. Weiss", "Chi-Wing Chow", "B. Mark Evers"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0019882.g002", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Knockdown_of_NFAT_blocked_PMA_Io_induced_TRAIL_expression_in_HT29_cells_/445189", "title"=>"Knockdown of NFAT blocked PMA/Io-induced TRAIL expression in HT29 cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 18:24:11"}
  • {"files"=>["https://ndownloader.figshare.com/files/775085"], "description"=>"<p>(<b>A</b>) Nucleotide sequences of the putative NFAT binding sites in the human TRAIL promotor. The 1371 bp TRAIL promoter contains five putative NFAT binding sites (N1, N2, N3, N4, and N5). The numbers on the left are the nucleotide positions relative to the transcriptional start site. (<b>B</b>) HT29 cells were treated with or without PMA/Io for 60 min and nuclear protein extracted; EMSA was performed with <sup>32</sup>P-labeled probes spanning each of the five NFAT binding sites (N1, N2, N3, N4, and N5) in the human TRAIL promoter. (<b>C</b>) Specific binding of various NFAT isoforms with probe N4 or N5 was determined by addition of antibodies to either NFATc1, NFATc2, NFATc3, or NFATc4. Specific binding of NFAT was also confirmed by cold competition using unlabeled wild type (WT) and mutant probes at 100-fold molar excess. (<b>D</b>) Caco-2 cells were transfected with constructs containing −35 or −165 TRAIL promoter sequences together with either control plasmid pDF30, or Flag-tagged NFATc1, respectively. At 48 h posttransfection, cells were harvested; and luciferase activity was assayed. All results were normalized for transfection efficiency using the pRL-Tk-luc plasmid (Promega). (Data shown as mean ± standard error of the mean; *, <i>P</i><0.05, DF30 or NFATc1 plus −165 bp promoter vs. DF30 or NFATc1 plus −35 promoter, respectively; <sup>+</sup>, <i>P</i><0.05, DF30 plus −165 bp promoter vs. NFATc1 plus −165 bp promoter).</p>", "links"=>[], "tags"=>["nfat", "binding", "sites", "did", "nfatc1", "promoter"], "article_id"=>445447, "categories"=>["Physiology", "Chemistry", "Genetics", "Developmental Biology"], "users"=>["Qingding Wang", "Yuning Zhou", "Heidi L. Weiss", "Chi-Wing Chow", "B. Mark Evers"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0019882.g004", "stats"=>{"downloads"=>2, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Deletion_of_NFAT_binding_sites_did_not_eliminate_NFATc1_increased_TRAIL_promoter_activity_/445447", "title"=>"Deletion of NFAT binding sites did not eliminate NFATc1 increased TRAIL promoter activity.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 18:25:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/775210"], "description"=>"<p>(<b>A</b>) Double-stranded oligonucleotides corresponding to all of the potential transcriptional binding sites in human TRAIL promoter −169 to −33 were synthesized and used for DNA binding analysis. The putative Sp1 binding sequences are boxed, which are also overlapped with other transcription factor binding sequences. (<b>B</b>) HT29 cells were treated with or without PMA/Io for 60 min and nuclear protein extracted. Double-stranded oligonucleotides (as shown in A) were radiolabeled and tested for DNA binding by EMSA. The arrows indicated the increased binding complexes. (<b>C</b>) Total RNA from HT29 cells stably transfected with control (CON) or four individual shRNAs targeting human NFATc1 (clones 33, 34, 35, 36) was extracted and RT-PCR performed for NFATc1 mRNA expression. (<b>D and E</b>) Nuclear protein (D) or total protein (E) from HT29 cells stably transfected with control (CON) or NFATc1 shRNA (clones 33, 34, 36) was extracted. EMSA was performed (D) using probe 4 and probe 5 (sequences shown in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0019882#pone-0019882-g006\" target=\"_blank\">Figure 6</a>). Western blotting was performed for the analysis of TRAIL expression (E); membranes were stripped and re-probed with anti-β-actin to confirm equal loading. TRAIL signals from two separate experiments were quantitated densitometrically and expressed as fold-change with respect to β-actin (Data shown as mean ± standard error of the mean).</p>", "links"=>[], "tags"=>["nfatc1", "responsive", "sequences"], "article_id"=>445579, "categories"=>["Physiology", "Chemistry", "Genetics", "Developmental Biology"], "users"=>["Qingding Wang", "Yuning Zhou", "Heidi L. Weiss", "Chi-Wing Chow", "B. Mark Evers"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0019882.g005", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Identification_of_NFATc1_responsive_sequences_in_the_TRAIL_promoter_/445579", "title"=>"Identification of NFATc1 responsive sequences in the TRAIL promoter.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 18:26:16"}
  • {"files"=>["https://ndownloader.figshare.com/files/775324"], "description"=>"<p>HT29 cells were transiently transfected with control siRNA or siRNA targeting NFATc1. Nuclear protein was extracted and EMSAs performed. Nuclear extracts were incubated with <sup>32</sup>P-labeled probe 4 (<b>A</b>) or probe 5 (<b>B</b>) alone or in the presence of unlabeled wild type (<i>cold</i>) probe 4 or probe 5, the Sp1 oligonucleotide, the NFAT oligonucleotide, or the ETS oligonucleotide, respectively. (<b>C</b>) HT29 cells were subjected to ChIP assay; soluble chromatin was prepared from HT29 cells transfected with control siRNA or siRNA targeting NFATc1 and immunoprecipitated with Sp1 antibody or IgG. Total (Input) and immunoprecipitated DNAs were then PCR-amplified using primer pairs covering the Sp1 binding sites within the human TRAIL promoter. (<b>D</b>) HT29 cells were treated with the Sp1 inhibitor mithramycin for 24 h and total protein extracted and Western blotting performed using anti-TRAIL antibody. (<b>E</b>) HT29 cells were transiently transfected with control siRNA or siRNA targeting Sp1. Forty-eight h after transfection, total protein was extracted and Western blotting performed to assess TRAIL expression (left panel) or to confirm knockdown of Sp1 (right panel). Membranes were stripped and re-probed with anti-β-actin antibody to confirm equal loading.</p>", "links"=>[], "tags"=>["mediated", "nfatc1"], "article_id"=>445695, "categories"=>["Physiology", "Chemistry", "Genetics", "Developmental Biology"], "users"=>["Qingding Wang", "Yuning Zhou", "Heidi L. Weiss", "Chi-Wing Chow", "B. Mark Evers"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0019882.g006", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sp1_mediated_NFATc1_regulation_of_TRAIL_expression_/445695", "title"=>"Sp1 mediated NFATc1 regulation of TRAIL expression.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 18:26:54"}

PMC Usage Stats | Further Information

  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"16", "unique-ip"=>"19", "pdf"=>"8", "year"=>"2011", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"50", "year"=>"2011", "pdf"=>"29", "unique-ip"=>"46", "figure"=>"19", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"29", "unique-ip"=>"26", "pdf"=>"13", "year"=>"2011", "figure"=>"17", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"11", "year"=>"2011", "pdf"=>"4", "unique-ip"=>"12", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"16", "unique-ip"=>"16", "pdf"=>"9", "year"=>"2011", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"15", "year"=>"2011", "pdf"=>"8", "unique-ip"=>"16", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"29", "unique-ip"=>"24", "pdf"=>"23", "year"=>"2011", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"10", "year"=>"2011", "pdf"=>"5", "unique-ip"=>"10", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"20", "unique-ip"=>"20", "pdf"=>"10", "year"=>"2012", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"14", "year"=>"2012", "pdf"=>"8", "unique-ip"=>"16", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"22", "unique-ip"=>"22", "pdf"=>"5", "year"=>"2012", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"12", "year"=>"2012", "pdf"=>"6", "unique-ip"=>"15", "figure"=>"11", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"21", "unique-ip"=>"20", "pdf"=>"9", "year"=>"2012", "figure"=>"6", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"8", "year"=>"2012", "pdf"=>"4", "unique-ip"=>"7", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"9", "full-text"=>"14", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"19", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"1", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"18", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"2", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"10", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"2", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"1", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"3", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"0", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"7", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"12", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"3", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"17", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"5", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"14", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2011-01-01T00:00:00Z", "end_date"=>"2011-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[304, 568, 702, 818, 927, 1027, 1118, 1206, 1285, 1357, 1427, 1500, 1564, 1636, 1705, 1773, 1840, 1909, 1974, 2039, 2106, 2170, 2234, 2296, 2358, 2423, 2484, 2546, 2606, 2673, 2734, 2795, 2857, 2921, 2984, 3046, 3100]}, {"subject_area"=>"/Biology and life sciences/Developmental biology", "average_usage"=>[301, 573, 717, 840, 956, 1063, 1159, 1248, 1327, 1406, 1478, 1557, 1626, 1700, 1772, 1843, 1910, 1980, 2052, 2124, 2192, 2263, 2328, 2391, 2443, 2496, 2564, 2629, 2698, 2771, 2834, 2897, 2959, 3021, 3077, 3144, 3193]}]}
Loading … Spinner
There are currently no alerts