RIG-I Is Required for the Inhibition of Measles Virus by Retinoids
Publication Date
July 19, 2011
Journal
PLOS ONE
Authors
Kaitlin J. Soye, Claire Trottier, Chris D. Richardson, Brian J. Ward, et al
Volume
6
Issue
7
Pages
e22323
DOI
https://dx.plos.org/10.1371/journal.pone.0022323
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0022323
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/21811588
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3139622
Europe PMC
http://europepmc.org/abstract/MED/21811588
Web of Science
000292929500054
Scopus
79960458276
Mendeley
http://www.mendeley.com/research/rigi-required-inhibition-measles-virus-retinoids
Events
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Mendeley | Further Information

{"title"=>"RIG-I is required for the inhibition of measles virus by retinoids", "type"=>"journal", "authors"=>[{"first_name"=>"Kaitlin J.", "last_name"=>"Soye", "scopus_author_id"=>"16308282000"}, {"first_name"=>"Claire", "last_name"=>"Trottier", "scopus_author_id"=>"24339337000"}, {"first_name"=>"Chris D.", "last_name"=>"Richardson", "scopus_author_id"=>"7402506135"}, {"first_name"=>"Brian J.", "last_name"=>"Ward", "scopus_author_id"=>"55617561700"}, {"first_name"=>"Wilson H.", "last_name"=>"Miller", "scopus_author_id"=>"7406058330"}], "year"=>2011, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"21811588", "sgr"=>"79960458276", "doi"=>"10.1371/journal.pone.0022323", "scopus"=>"2-s2.0-79960458276", "pui"=>"362161282", "issn"=>"19326203"}, "id"=>"a1ce12b9-650f-38fa-bf22-929696acafd4", "abstract"=>"Vitamin A can significantly decrease measles-associated morbidity and mortality. Vitamin A can inhibit the replication of measles virus (MeV) in vitro through an RARα- and type I interferon (IFN)-dependent mechanism. Retinoid-induced gene I (RIG-I) expression is induced by retinoids, activated by MeV RNA and is important for IFN signaling. We hypothesized that RIG-I is central to retinoid-mediated inhibition of MeV in vitro. We demonstrate that RIG-I expression is increased in cells treated with retinoids and infected with MeV. The central role of RIG-I in the retinoid-anti-MeV effect was demonstrated in the Huh-7/7.5 model; the latter cells having non-functional RIG-I. RAR-dependent retinoid signaling was required for the induction of RIG-I by retinoids and MeV. Retinoid signaling was also found to act in combination with IFN to induce high levels of RIG-I expression. RIG-I promoter activation required both retinoids and MeV, as indicated by markers of active chromatin. IRF-1 is known to be regulated by retinoids and MeV, but we found recruitment of IRF-1 to the RIG-I promoter by retinoids alone. Using luciferase expression constructs, we further demonstrated that the IRF-1 response element of RIG-I was required for RIG-I activation by retinoids or IFN. These results reveal that retinoid treatment and MeV infection induces significant RIG-I. RIG-I is required for the retinoid-MeV antiviral response. The induction is dependent on IFN, retinoids and IRF-1.", "link"=>"http://www.mendeley.com/research/rigi-required-inhibition-measles-virus-retinoids", "reader_count"=>15, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Researcher"=>5, "Student > Ph. D. Student"=>4, "Student > Postgraduate"=>1, "Student > Master"=>1, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Researcher"=>5, "Student > Ph. D. Student"=>4, "Student > Postgraduate"=>1, "Student > Master"=>1, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Agricultural and Biological Sciences"=>6, "Medicine and Dentistry"=>6, "Neuroscience"=>1, "Chemistry"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>6}, "Neuroscience"=>{"Neuroscience"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>6}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/755356"], "description"=>"<p>(A) Cells were infected with MeV at an MOI of 0.1 in the presence of 1 µM ATRA or DMSO, and either IFNαβ-receptor blocking antibodies or isotype control. RNA was extracted at 24 h and RIG-I expression was measured by qPCR. Data presented are representative of 2 experiments performed in triplicate (N = 2). (B) Transwell membrane inserts with 0.02 µm pores were used to separate the infected cells from the uninfected, bystander cells in the inner chamber <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0022323#pone.0022323-Trottier2\" target=\"_blank\">[9]</a>. (C) Cells from transwell-free control wells and the inner chamber bystander cells were harvested after 48 hours and RIG-I mRNA was measured by qPCR. Data presented are representative of three experiments performed in triplicate (N = 3). (D) Supernatants from the control wells and the inner chambers of the transwells were used to treat fresh U937 cells with either IFNαβ-receptor blocking antibody or isotype control antibody. Following 24 hours of incubation, RIG-I expression was assessed by qPCR. Data presented are representative of three experiments performed in triplicate (N = 3). **p<0.01, ***p<0.001.</p>", "links"=>[], "tags"=>["atra", "induces", "soluble", "ifn", "elicit"], "article_id"=>425723, "categories"=>["Microbiology", "Virology", "Infectious Diseases", "Immunology"], "users"=>["Kaitlin J. Soye", "Claire Trottier", "Chris D. Richardson", "Brian J. Ward", "Wilson H. Miller Jr."], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0022323.g003", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_MeV_with_ATRA_induces_the_soluble_factor_IFN_to_elicit_the_expression_of_RIG_I_/425723", "title"=>"MeV with ATRA induces the soluble factor IFN to elicit the expression of RIG-I.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 16:38:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/755281"], "description"=>"<p>(A) Huh7 and Huh7.5 cells were infected MeV at an MOI of 0.01 and treated with 1 µM ATRA or DMSO. Whole cell lysates were harvested after 48 hours and viral titers were measured by plaque assay. (B) Huh7 cells were infected with MeV at an MOI of 0.01 and treated with 1 µM ATRA or DMSO and transfected with the control plasmid or RIG-I dominant negative (RIG-IC) expression construct. Whole cell lysates were harvested after 48 hours and viral titers were measured by plaque assay. (C) Huh7.5 cells were transfected with the control plasmid or RIG-I expression construct and infected with MeV at an MOI of 0.01. Whole cell lysates were harvested after 48hr and viral titers were measured by plaque assay. Data represent two experiments performed in triplicate (N = 2). **p<0.01, ***p<0.001.</p>", "links"=>[], "tags"=>["inhibition", "mev"], "article_id"=>425658, "categories"=>["Microbiology", "Virology", "Infectious Diseases", "Immunology"], "users"=>["Kaitlin J. Soye", "Claire Trottier", "Chris D. Richardson", "Brian J. Ward", "Wilson H. Miller Jr."], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0022323.g002", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_RIG_I_necessary_for_inhibition_of_MeV_by_ATRA_/425658", "title"=>"RIG-I necessary for inhibition of MeV by ATRA.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 16:37:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/755586"], "description"=>"<p>(A) Diagram of the RIG-I promoter showing the known IRF1 binding site. Arrows represent the site of primers used in ChIP experiments. (B) RARα and RXR were immunoprecipitated from cells treated with 1 µM ATRA or DMSO (N = 2) (C) U937 cells were infected with MeV at an MOI of 0.1 and/or treated with 1 µM ATRA or DMSO for 24 hours. 1000 U/ml of IFNβ was used as a positive control. These samples were then immunoprecipitated RARα primary antibodies. The pulled-down DNA was analyzed by qPCR using primers specific for the RIG-I promoter as described in the <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0022323#s4\" target=\"_blank\">materials and methods</a>. Data presented are representative of three experiments performed in triplicate (N = 3). *p<0.05, **p<0.01, ***p<0.001.</p>", "links"=>[], "tags"=>["receptors", "rig-i"], "article_id"=>425959, "categories"=>["Microbiology", "Virology", "Infectious Diseases", "Immunology"], "users"=>["Kaitlin J. Soye", "Claire Trottier", "Chris D. Richardson", "Brian J. Ward", "Wilson H. Miller Jr."], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0022323.g005", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Retinoic_acid_nuclear_receptors_bind_to_the_RIG_I_promoter_/425959", "title"=>"Retinoic acid nuclear receptors bind to the RIG-I promoter.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 16:39:17"}
  • {"files"=>["https://ndownloader.figshare.com/files/755209"], "description"=>"<p>(A) U937 cells were infected with MeV at an MOI of 0.1 and treated with increasing doses of ATRA or DMSO for 24 hours. Some samples were also treated with 1000 U/mL IFNβ for 24 hours, with or without ATRA. RNA was extracted and analyzed for RIG-I expression by qPCR. Data presented are representative of three experiments performed in triplicate (N = 3). (B) U937 cells were infected with MeV at an MOI of 0.1 and treated with ATRA or DMSO (1 µM) for 48 hours, or with 2000 U/mL IFNβ as a positive control. Samples were analyzed by western blot for RIG-I and β-actin expression and quantified. ***p<0.001.</p>", "links"=>[], "tags"=>["regulated", "mev"], "article_id"=>425575, "categories"=>["Microbiology", "Virology", "Infectious Diseases", "Immunology"], "users"=>["Kaitlin J. Soye", "Claire Trottier", "Chris D. Richardson", "Brian J. Ward", "Wilson H. Miller Jr."], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0022323.g001", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_RIG_I_expression_is_regulated_by_MeV_and_ATRA_/425575", "title"=>"RIG-I expression is regulated by MeV and ATRA.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 16:37:12"}
  • {"files"=>["https://ndownloader.figshare.com/files/755667"], "description"=>"<p>U937 cells were infected with MeV at an MOI of 0.1 and/or treated with 1 µM ATRA or DMSO for 24 hours. 1000 U/ml of IFNβ was used as a positive control. These samples were then immunoprecipitated the following primary antibodies (A) Acetylate Histone H3 (B) Pol II (C) IRF-1. The pulled-down DNA was analyzed by qPCR using primers specific for the RIG-I promoter as described in the <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0022323#s4\" target=\"_blank\">materials and methods</a>. Data presented are representative of experiments performed in triplicate between two and three times (N = 2–3). *p<0.05, **p<0.01, ***p<0.001.</p>", "links"=>[], "tags"=>["rig-i", "promoter", "mev", "retinoid"], "article_id"=>426036, "categories"=>["Microbiology", "Virology", "Infectious Diseases", "Immunology"], "users"=>["Kaitlin J. Soye", "Claire Trottier", "Chris D. Richardson", "Brian J. Ward", "Wilson H. Miller Jr."], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0022323.g006", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Activation_of_RIG_I_promoter_only_upon_combination_of_MeV_infection_and_retinoid_treatment_/426036", "title"=>"Activation of RIG-I promoter only upon combination of MeV infection and retinoid treatment.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 16:39:42"}
  • {"files"=>["https://ndownloader.figshare.com/files/755509"], "description"=>"<p>(A) NB4 and R4 cells were infected with MeV at an MOI of 0.01, treated with 1 µM ATRA or DMSO and/or treated with 1000 U/mL IFNβ. After 48 hours, samples were harvested and analyzed for RIG-I expression and (B) RARβ expression by qPCR. Data presented are representative of two experiments performed in triplicate (N = 2). (C) U937 cells were infected at an MOI of 0.1 for 24 hours in the presence of 10 nM ATRA and/or 1000 nM of the RARα-selective antagonist RO 41-5253 (RO). Samples were analyzed for RIG-I expression by qPCR. Data presented are representative of three experiments performed in triplicate (N = 3). **p<0.01, ***p<0.001.</p>", "links"=>[], "tags"=>["rar"], "article_id"=>425885, "categories"=>["Microbiology", "Virology", "Infectious Diseases", "Immunology"], "users"=>["Kaitlin J. Soye", "Claire Trottier", "Chris D. Richardson", "Brian J. Ward", "Wilson H. Miller Jr."], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0022323.g004", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_RIG_I_expression_required_RAR_signaling_/425885", "title"=>"RIG-I expression required RAR signaling.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 16:38:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/755749"], "description"=>"<p>(A) U937 cells were infected with MeV at an MOI of 0.1 and treated with increasing doses of ATRA or DMSO for 24 hours. RNA was extracted and analyzed for IRF1 expression by qPCR. Data presented are representative of three experiments performed in triplicate (N = 3). (B) U937 cells were infected with MeV at an MOI of 0.1 and/or treated with 1 µM ATRA or DMSO for 24 hours. Cells were also treated with 1000 U/ml of IFNβ as a positive control. Samples were analyzed by western blot for IRF-1 and β-actin expression. (C) NB4 and R4 cells were infected with MeV at an MOI of 0.01, treated with 1 µM ATRA or DMSO and/or treated with 1000 U/mL IFNβ. After 48 hours, samples were harvested and analyzed for IRF-1 expression by qPCR. Data presented are representative of three experiments performed in triplicate (N = 3). (D) A schematic of the RIG-I promoter constructs including the full length construct pRIGI-full and the IRF-binding mutant pRIGI-IRFmut. (E) HeLa cells were transfected with 3 µg of pRIGI-full or pRIGI-IRFmut construct and 0.5 µg of Renilla control. The cells were then treated with 1 µM ATRA or 1000 U/ml of IFNβ for an additional 24 hours. Samples were then analyzed for dual-luciferase expression. *p<0.05, **p<0.01, ***p<0.001.</p>", "links"=>[], "tags"=>["regulated", "mev", "retinoid", "contributing", "rig-i", "antiviral"], "article_id"=>426117, "categories"=>["Microbiology", "Virology", "Infectious Diseases", "Immunology"], "users"=>["Kaitlin J. Soye", "Claire Trottier", "Chris D. Richardson", "Brian J. Ward", "Wilson H. Miller Jr."], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0022323.g007", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_IRF_1_is_regulated_by_MeV_infection_and_retinoid_treatment_contributing_to_the_RIG_I_antiviral_response_/426117", "title"=>"IRF-1 is regulated by MeV infection and retinoid treatment contributing to the RIG-I antiviral response.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 16:40:06"}

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Relative Metric

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