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• {"link"=>{"url"=>"http://www.citeulike.org/user/Laur705/article/9738311"}, "post_time"=>"2011-09-04 18:50:01", "tag"=>["cnidaria", "gene", "gene_expression", "genome", "genome_sequence", "genome_sequencing", "next_generation_sequencing", "siphonophore"], "linkout"=>{"type"=>"DOI", "url"=>"http://dx.doi.org/10.1371/journal.pone.0022953"}, "username"=>"Laur705", "article_id"=>"9738311"}

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{"title"=>"Differential gene expression in the siphonophore Nanomia bijuga (Cnidaria) assessed with multiple Next-Generation sequencing workflows", "type"=>"journal", "authors"=>[{"first_name"=>"Stefan", "last_name"=>"Siebert", "scopus_author_id"=>"36194199900"}, {"first_name"=>"Mark D.", "last_name"=>"Robinson", "scopus_author_id"=>"56433742400"}, {"first_name"=>"Sophia C.", "last_name"=>"Tintori", "scopus_author_id"=>"36194601500"}, {"first_name"=>"Freya", "last_name"=>"Goetz", "scopus_author_id"=>"37033992000"}, {"first_name"=>"Rebecca R.", "last_name"=>"Helm", "scopus_author_id"=>"36182419800"}, {"first_name"=>"Stephen A.", "last_name"=>"Smith", "scopus_author_id"=>"55702297900"}, {"first_name"=>"Nathan", "last_name"=>"Shaner", "scopus_author_id"=>"6507174681"}, {"first_name"=>"Steven H D", "last_name"=>"Haddock", "scopus_author_id"=>"7003746627"}, {"first_name"=>"Casey W.", "last_name"=>"Dunn", "scopus_author_id"=>"9736081000"}], "year"=>2011, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-79960896843", "sgr"=>"79960896843", "pui"=>"362239070", "isbn"=>"1932-6203 (Linking)", "pmid"=>"21829563", "doi"=>"10.1371/journal.pone.0022953"}, "id"=>"d6615c35-d5b2-379b-877b-04ef95033258", "abstract"=>"We investigated differential gene expression between functionally specialized feeding polyps and swimming medusae in the siphonophore Nanomia bijuga (Cnidaria) with a hybrid long-read/short-read sequencing strategy. We assembled a set of partial gene reference sequences from long-read data (Roche 454), and generated short-read sequences from replicated tissue samples that were mapped to the references to quantify expression. We collected and compared expression data with three short-read expression workflows that differ in sample preparation, sequencing technology, and mapping tools. These workflows were Illumina mRNA-Seq, which generates sequence reads from random locations along each transcript, and two tag-based approaches, SOLiD SAGE and Helicos DGE, which generate reads from particular tag sites. Differences in expression results across workflows were mostly due to the differential impact of missing data in the partial reference sequences. When all 454-derived gene reference sequences were considered, Illumina mRNA-Seq detected more than twice as many differentially expressed (DE) reference sequences as the tag-based workflows. This discrepancy was largely due to missing tag sites in the partial reference that led to false negatives in the tag-based workflows. When only the subset of reference sequences that unambiguously have tag sites was considered, we found broad congruence across workflows, and they all identified a similar set of DE sequences. Our results are promising in several regards for gene expression studies in non-model organisms. First, we demonstrate that a hybrid long-read/short-read sequencing strategy is an effective way to collect gene expression data when an annotated genome sequence is not available. Second, our replicated sampling indicates that expression profiles are highly consistent across field-collected animals in this case. Third, the impacts of partial reference sequences on the ability to detect DE can be mitigated through workflow choice and deeper reference sequencing.", "link"=>"http://www.mendeley.com/research/differential-gene-expression-siphonophore-nanomia-bijuga-cnidaria-assessed-multiple-nextgeneration-s", "reader_count"=>88, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>12, "Researcher"=>23, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>28, "Student > Postgraduate"=>3, "Student > Master"=>8, "Other"=>2, "Student > Bachelor"=>4, "Lecturer"=>2, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>12, "Researcher"=>23, "Student > Doctoral Student"=>4, "Student > Ph. D. 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• http://doi.org/10.1038/nprot.2013.099
• http://doi.org/10.1534/g3.115.020164
• http://doi.org/10.1093/molbev/msu410
• http://doi.org/10.1007/s00227-020-3662-6
• http://doi.org/10.1186/1471-2164-13-474
• http://doi.org/10.1002/bies.201100145
• http://doi.org/10.1371/journal.pone.0087737
• http://doi.org/10.1186/s12859-016-1366-1
• http://doi.org/10.1186/1471-2164-14-266
• http://doi.org/10.1093/gbe/evw155
• http://doi.org/10.1186/s13227-015-0018-2
• http://doi.org/10.3390/md14040075
• http://doi.org/10.1093/molbev/mst074
• http://doi.org/10.1093/icb/ict068
• http://doi.org/10.1371/journal.pone.0073435
• http://doi.org/10.1093/icb/icu080
• http://doi.org/10.1002/jez.b.22944
• http://doi.org/10.1093/gbe/evv153
• http://doi.org/10.1093/nar/gks540
• http://doi.org/10.1186/1471-2164-15-71
• http://doi.org/10.1111/1755-0998.12415
• http://doi.org/10.1371/journal.pone.0053214
• http://doi.org/10.1111/brv.12478
• http://doi.org/10.1002/jez.b.22629
• http://doi.org/10.1007/s12268-012-0177-9
• http://doi.org/10.2108/zs150186
• http://doi.org/10.1093/icb/icu027
• http://doi.org/10.1016/j.gde.2016.07.006
• http://doi.org/10.1186/1471-2164-15-406
• http://doi.org/10.1186/1471-2164-13-6

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Figshare

• {"files"=>["https://ndownloader.figshare.com/files/751142"], "description"=>"<p>Multidimensional scaling plots indicate low degrees of differential expression between samples of the same tissue type originating from different specimen (dimension 2) and higher degrees of differential expression when comparing different tissues types (dimension 1). N: nectophore sample, G: gastrozooid sample. The full set of reference sequences was considered.</p>", "links"=>[], "tags"=>["samples", "specimen", "workflows"], "article_id"=>421504, "categories"=>["Biological Sciences", "Physics", "Inorganic Chemistry", "Biochemistry", "Biophysics", "Genetics", "Molecular Biology", "Ecology"], "users"=>["Stefan Siebert", "Mark D. Robinson", "Sophia C. Tintori", "Freya Goetz", "Rebecca R. Helm", "Stephen A. Smith", "Nathan Shaner", "Steven H. D. Haddock", "Casey W. Dunn"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0022953.g006", "stats"=>{"downloads"=>3, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Differential_expression_across_samples_from_different_specimen_in_different_workflows_A_8211_C_/421504", "title"=>"Differential expression across samples from different specimen in different workflows (A–C).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-07-29 00:25:04"}
• {"files"=>["https://ndownloader.figshare.com/files/751054"], "description"=>"<p>Fractions of mapped reads are shown for all reference sequences (<b>A</b>) and the subset of sequences with the 3′-most <i>NlaIII</i> site (<b>B</b>). Genes are sorted along the x axis in descending order of the number of mapped reads.</p>", "links"=>[], "tags"=>["cumulative", "mapped", "reads"], "article_id"=>421420, "categories"=>["Biological Sciences", "Physics", "Inorganic Chemistry", "Biochemistry", "Biophysics", "Genetics", "Molecular Biology", "Ecology"], "users"=>["Stefan Siebert", "Mark D. Robinson", "Sophia C. Tintori", "Freya Goetz", "Rebecca R. Helm", "Stephen A. Smith", "Nathan Shaner", "Steven H. D. Haddock", "Casey W. Dunn"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0022953.g005", "stats"=>{"downloads"=>3, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_cumulative_fraction_of_total_mapped_reads_across_reference_sequences_/421420", "title"=>"The cumulative fraction of total mapped reads across reference sequences.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-07-29 00:23:40"}
• {"files"=>["https://ndownloader.figshare.com/files/750848"], "description"=>"<p>Analyses of all reference sequences (<b>A, B</b>) and analyses of the subset of sequences with the 3′-most <i>NlaIII</i> site (<b>C, D</b>). (<b>A, C</b>) The effect of the Z threshold on the number of genes found to have differential gene expression. The relatively flat lines in all cases indicate little sensitivity to Z threshold. (<b>B</b>) Proportional Venn diagram of the number of sequences with significant differential expression (Z>4.71) in analyses of all reference sequences. (<b>D</b>) Proportional Venn diagram of the number of sequences with significant differential expression (Z>4.38) in analyses of the subset of sequences with the primary tag site. Areas in Venn diagrams are approximate. Dashed lines in <b>A,C</b> indicate Z-values used in <b>B, D</b>. The same color code applies to all figures.</p>", "links"=>[], "tags"=>["overlap", "platforms", "sequences", "differential"], "article_id"=>421210, "categories"=>["Biological Sciences", "Physics", "Inorganic Chemistry", "Biochemistry", "Biophysics", "Genetics", "Molecular Biology", "Ecology"], "users"=>["Stefan Siebert", "Mark D. Robinson", "Sophia C. Tintori", "Freya Goetz", "Rebecca R. Helm", "Stephen A. Smith", "Nathan Shaner", "Steven H. D. Haddock", "Casey W. Dunn"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0022953.g003", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_number_and_overlap_across_platforms_of_reference_sequences_identified_to_have_differential_expression_DE_/421210", "title"=>"The number and overlap across platforms of reference sequences identified to have differential expression (DE).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-07-29 00:20:10"}
• {"files"=>["https://ndownloader.figshare.com/files/750647"], "description"=>"<p>(<b>A</b>) Paired samples of young nectophores (<b>B</b>) and young gastrozooids (<b>C</b>) were removed from each of three remotely operated vehicle-collected specimens (see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0022953#pone.0022953.s013\" target=\"_blank\">video S1</a>). n: nectophore, g: gastrozooid, s: stem of the colony. Frames in (<b>A</b><i>)</i> indicate regions shown in (<b>B</b><i>)</i> and (<b>C</b><i>)</i>. Numbers indicate the sampled zooids.</p>", "links"=>[], "tags"=>["sampled", "siphonophore"], "article_id"=>421012, "categories"=>["Biological Sciences", "Physics", "Inorganic Chemistry", "Biochemistry", "Biophysics", "Genetics", "Molecular Biology", "Ecology"], "users"=>["Stefan Siebert", "Mark D. Robinson", "Sophia C. Tintori", "Freya Goetz", "Rebecca R. Helm", "Stephen A. Smith", "Nathan Shaner", "Steven H. D. Haddock", "Casey W. Dunn"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0022953.g001", "stats"=>{"downloads"=>2, "page_views"=>25, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Tissues_sampled_from_the_siphonophore_Nanomia_bijuga_/421012", "title"=>"Tissues sampled from the siphonophore <i>Nanomia bijuga</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-07-29 00:16:52"}
• {"files"=>["https://ndownloader.figshare.com/files/378578", "https://ndownloader.figshare.com/files/378610", "https://ndownloader.figshare.com/files/378647", "https://ndownloader.figshare.com/files/378673", "https://ndownloader.figshare.com/files/378703", "https://ndownloader.figshare.com/files/378738", "https://ndownloader.figshare.com/files/379540", "https://ndownloader.figshare.com/files/379645", "https://ndownloader.figshare.com/files/379739", "https://ndownloader.figshare.com/files/379765", "https://ndownloader.figshare.com/files/379788", "https://ndownloader.figshare.com/files/379820", "https://ndownloader.figshare.com/files/379846"], "description"=>"<div><p>We investigated differential gene expression between functionally specialized feeding polyps and swimming medusae in the siphonophore <em>Nanomia bijuga</em> (Cnidaria) with a hybrid long-read/short-read sequencing strategy. We assembled a set of partial gene reference sequences from long-read data (Roche 454), and generated short-read sequences from replicated tissue samples that were mapped to the references to quantify expression. We collected and compared expression data with three short-read expression workflows that differ in sample preparation, sequencing technology, and mapping tools. These workflows were Illumina mRNA-Seq, which generates sequence reads from random locations along each transcript, and two tag-based approaches, SOLiD SAGE and Helicos DGE, which generate reads from particular tag sites. Differences in expression results across workflows were mostly due to the differential impact of missing data in the partial reference sequences. When all 454-derived gene reference sequences were considered, Illumina mRNA-Seq detected more than twice as many differentially expressed (DE) reference sequences as the tag-based workflows. This discrepancy was largely due to missing tag sites in the partial reference that led to false negatives in the tag-based workflows. When only the subset of reference sequences that unambiguously have tag sites was considered, we found broad congruence across workflows, and they all identified a similar set of DE sequences. Our results are promising in several regards for gene expression studies in non-model organisms. First, we demonstrate that a hybrid long-read/short-read sequencing strategy is an effective way to collect gene expression data when an annotated genome sequence is not available. Second, our replicated sampling indicates that expression profiles are highly consistent across field-collected animals in this case. Third, the impacts of partial reference sequences on the ability to detect DE can be mitigated through workflow choice and deeper reference sequencing.</p> </div>", "links"=>[], "tags"=>["differential", "siphonophore", "assessed", "next-generation", "sequencing", "workflows"], "article_id"=>134826, "categories"=>["Biological Sciences", "Physics", "Inorganic Chemistry", "Biochemistry", "Biophysics", "Genetics", "Molecular Biology", "Ecology"], "users"=>["Stefan Siebert", "Mark D. Robinson", "Sophia C. Tintori", "Freya Goetz", "Rebecca R. Helm", "Stephen A. Smith", "Nathan Shaner", "Steven H. D. Haddock", "Casey W. Dunn"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0022953.s001", "https://dx.doi.org/10.1371/journal.pone.0022953.s002", "https://dx.doi.org/10.1371/journal.pone.0022953.s003", "https://dx.doi.org/10.1371/journal.pone.0022953.s004", "https://dx.doi.org/10.1371/journal.pone.0022953.s005", "https://dx.doi.org/10.1371/journal.pone.0022953.s006", "https://dx.doi.org/10.1371/journal.pone.0022953.s007", "https://dx.doi.org/10.1371/journal.pone.0022953.s008", "https://dx.doi.org/10.1371/journal.pone.0022953.s009", "https://dx.doi.org/10.1371/journal.pone.0022953.s010", "https://dx.doi.org/10.1371/journal.pone.0022953.s011", "https://dx.doi.org/10.1371/journal.pone.0022953.s012", "https://dx.doi.org/10.1371/journal.pone.0022953.s013"], "stats"=>{"downloads"=>26, "page_views"=>277, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Differential_Gene_Expression_in_the_Siphonophore_Nanomia_bijuga_Cnidaria_Assessed_with_Multiple_Next_Generation_Sequencing_Workflows/134826", "title"=>"Differential Gene Expression in the Siphonophore <em>Nanomia bijuga</em> (Cnidaria) Assessed with Multiple Next-Generation Sequencing Workflows", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2011-07-29 01:20:26"}
• {"files"=>["https://ndownloader.figshare.com/files/751264"], "description"=>"<p>Expression analysis of isogroup03256 in developing nectophores (<b>A</b>) and gastrozooids (<b>B</b>). The transcript is localized in the basigaster, a region associated with nematogenesis, at the base of gastrozooids and detectable in very early stages of gastrozooid development (arrow). (<b>C</b>) Sense control with an unstained basigaster region. <i>In situ</i> hybridizations for nectophore specific transcripts have been performed successfully (data not shown). S: stem of the colony, b: basigaster region of the gastrozooid, pn: pneumatophore.</p>", "links"=>[], "tags"=>["characterisation"], "article_id"=>421637, "categories"=>["Biological Sciences", "Physics", "Inorganic Chemistry", "Biochemistry", "Biophysics", "Genetics", "Molecular Biology", "Ecology"], "users"=>["Stefan Siebert", "Mark D. Robinson", "Sophia C. Tintori", "Freya Goetz", "Rebecca R. Helm", "Stephen A. Smith", "Nathan Shaner", "Steven H. D. Haddock", "Casey W. Dunn"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0022953.g007", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_In_situ_characterisation_of_gene_expression_/421637", "title"=>"<i>In situ</i> characterisation of gene expression.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-07-29 00:27:17"}
• {"files"=>["https://ndownloader.figshare.com/files/750972"], "description"=>"<p>Number of DE sequences are plotted against subsampled library sizes considering the full reference (<b>A</b>) and the subset of sequences with the 3′-most <i>NlaIII</i> site (<b>B</b>). This enables comparison of significance across equivalent library sizes.</p>", "links"=>[], "tags"=>["curves", "genes", "differential", "reads"], "article_id"=>421336, "categories"=>["Biological Sciences", "Physics", "Inorganic Chemistry", "Biochemistry", "Biophysics", "Genetics", "Molecular Biology", "Ecology"], "users"=>["Stefan Siebert", "Mark D. Robinson", "Sophia C. Tintori", "Freya Goetz", "Rebecca R. Helm", "Stephen A. Smith", "Nathan Shaner", "Steven H. D. Haddock", "Casey W. Dunn"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0022953.g004", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Accumulation_curves_indicating_the_number_of_genes_with_significant_differential_expression_DE_when_short_reads_are_subsampled_/421336", "title"=>"Accumulation curves indicating the number of genes with significant differential expression (DE) when short reads are subsampled.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-07-29 00:22:16"}
• {"files"=>["https://ndownloader.figshare.com/files/750761"], "description"=>"<p>Read distribution is shown for a fibrillar collagen (isogroup06489, tblastx e-value 1e-87) for the three nectophore samples. Gastrozooid expression was much lower and not visible on the same scale. All three short-read workflows found significant differential expression for this gene. The gene is drawn in the 5′–3′ direction (4,864 bp). Height of the colored bars indicates the number of reads mapped to that location. Count data are not normalized, so differences in amplitude across samples can be due to differences in sequencing effort across samples. Reads above the line map in the sense direction, below the line in the antisense direction. Helicos DGE reads (red) are sense and unexpectedly tended to map to the 3′ end. Illumina mRNA-Seq reads (green) map to sense and antisense strand along the whole gene. The largest stack of reads for SOliD SAGE (blue) is adjacent to the 3′-most <i>NlaIII</i> cutting site.</p>", "links"=>[], "tags"=>["mapped", "short-read", "sequences"], "article_id"=>421125, "categories"=>["Biological Sciences", "Physics", "Inorganic Chemistry", "Biochemistry", "Biophysics", "Genetics", "Molecular Biology", "Ecology"], "users"=>["Stefan Siebert", "Mark D. Robinson", "Sophia C. Tintori", "Freya Goetz", "Rebecca R. Helm", "Stephen A. Smith", "Nathan Shaner", "Steven H. D. Haddock", "Casey W. Dunn"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0022953.g002", "stats"=>{"downloads"=>3, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Physical_distribution_of_mapped_short_read_sequences_across_an_example_transcript_/421125", "title"=>"Physical distribution of mapped short-read sequences across an example transcript.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-07-29 00:18:45"}

PMC Usage Stats | Further Information

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Relative Metric

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F1000Prime