A Novel Multiplex Real-Time PCR for the Identification of Mycobacteria Associated with Zoonotic Tuberculosis
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"A novel multiplex real-time pcr for the identification of mycobacteria associated with zoonotic tuberculosis", "type"=>"journal", "authors"=>[{"first_name"=>"Kate", "last_name"=>"Reddington", "scopus_author_id"=>"38761911800"}, {"first_name"=>"Justin", "last_name"=>"O'Grady", "scopus_author_id"=>"24767991700"}, {"first_name"=>"Siobhan", "last_name"=>"Dorai-Raj", "scopus_author_id"=>"35208415700"}, {"first_name"=>"Stefan", "last_name"=>"Niemann", "scopus_author_id"=>"7006827120"}, {"first_name"=>"Dick", "last_name"=>"van Soolingen", "scopus_author_id"=>"55114040800"}, {"first_name"=>"Thomas", "last_name"=>"Barry", "scopus_author_id"=>"7102200427"}], "year"=>2011, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"79961242178", "doi"=>"10.1371/journal.pone.0023481", "pui"=>"362302135", "pmid"=>"21858140", "scopus"=>"2-s2.0-79961242178", "issn"=>"19326203", "isbn"=>"1932-6203 (Electronic)\\n1932-6203 (Linking)"}, "id"=>"1c63e330-ab05-3adf-9555-cbcd3c66ec12", "abstract"=>"BACKGROUND: Tuberculosis (TB) is the leading cause of death worldwide from a single infectious agent. An ability to detect the Mycobacterium tuberculosis complex (MTC) in clinical material while simultaneously differentiating its members is considered important. This allows for the gathering of epidemiological information pertaining to the prevalence, transmission and geographical distribution of the MTC, including those MTC members associated with zoonotic TB infection in humans. Also differentiating between members of the MTC provides the clinician with inherent MTC specific drug susceptibility profiles to guide appropriate chemotherapy.\\n\\nMETHODOLOGY/PRINCIPAL FINDINGS: The aim of this study was to develop a multiplex real-time PCR assay using novel molecular targets to identify and differentiate between the phylogenetically closely related M. bovis, M. bovis BCG and M. caprae. The lpqT gene was explored for the collective identification of M. bovis, M. bovis BCG and M. caprae, the lepA gene was targeted for the specific identification of M. caprae and a Region of Difference 1 (RD1) assay was incorporated in the test to differentiate M. bovis BCG. The multiplex real-time PCR assay was evaluated on 133 bacterial strains and was determined to be 100% specific for the members of the MTC targeted.\\n\\nCONCLUSIONS/SIGNIFICANCE: The multiplex real-time PCR assay developed in this study is the first assay described for the identification and simultaneous differentiation of M. bovis, M. bovis BCG and M. caprae in one internally controlled reaction. Future validation of this multiplex assay should demonstrate its potential in the rapid and accurate diagnosis of TB caused by these three mycobacteria. Furthermore, the developed assay may be used in conjunction with a recently described multiplex real-time PCR assay for identification of the MTC and simultaneous differentiation of M. tuberculosis, M. canettii resulting in an ability to differentiate five of the eight members of the MTC.", "link"=>"http://www.mendeley.com/research/novel-multiplex-realtime-pcr-identification-mycobacteria-associated-zoonotic-tuberculosis", "reader_count"=>60, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Researcher"=>12, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>19, "Student > Postgraduate"=>6, "Student > Master"=>12, "Other"=>1, "Student > Bachelor"=>3, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Researcher"=>12, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>19, "Student > Postgraduate"=>6, "Student > Master"=>12, "Other"=>1, "Student > Bachelor"=>3, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Engineering"=>1, "Environmental Science"=>2, "Biochemistry, Genetics and Molecular Biology"=>4, "Agricultural and Biological Sciences"=>38, "Medicine and Dentistry"=>8, "Veterinary Science and Veterinary Medicine"=>3, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>8}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>38}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}, "Unspecified"=>{"Unspecified"=>1}, "Environmental Science"=>{"Environmental Science"=>2}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>3}}, "reader_count_by_country"=>{"Colombia"=>1, "Hong Kong"=>1, "South Africa"=>1, "Switzerland"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/79961242178"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/79961242178?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79961242178&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=79961242178&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/79961242178", "dc:identifier"=>"SCOPUS_ID:79961242178", "eid"=>"2-s2.0-79961242178", "dc:title"=>"A novel multiplex real-time pcr for the identification of mycobacteria associated with zoonotic tuberculosis", "dc:creator"=>"Reddington K.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"6", "prism:issueIdentifier"=>"8", "prism:pageRange"=>nil, "prism:coverDate"=>"2011-08-12", "prism:coverDisplayDate"=>"2011", "prism:doi"=>"10.1371/journal.pone.0023481", "citedby-count"=>"25", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"National University of Ireland Galway", "affiliation-city"=>"Galway", "affiliation-country"=>"Ireland"}], "pubmed-id"=>"21858140", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e23481", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0023481", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Journal Comments | Further Information

Counter

  • {"month"=>"8", "year"=>"2011", "pdf_views"=>"64", "xml_views"=>"7", "html_views"=>"212"}
  • {"month"=>"9", "year"=>"2011", "pdf_views"=>"58", "xml_views"=>"0", "html_views"=>"115"}
  • {"month"=>"10", "year"=>"2011", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"11", "year"=>"2011", "pdf_views"=>"54", "xml_views"=>"0", "html_views"=>"140"}
  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"41", "xml_views"=>"16", "html_views"=>"105"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"84", "xml_views"=>"45", "html_views"=>"143"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"49", "xml_views"=>"20", "html_views"=>"119"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"39", "xml_views"=>"12", "html_views"=>"118"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"49", "xml_views"=>"22", "html_views"=>"126"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"36", "xml_views"=>"0", "html_views"=>"140"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"31", "xml_views"=>"0", "html_views"=>"121"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"50", "xml_views"=>"0", "html_views"=>"148"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"31", "xml_views"=>"4", "html_views"=>"94"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"37", "xml_views"=>"0", "html_views"=>"117"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"32", "xml_views"=>"0", "html_views"=>"128"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"36", "xml_views"=>"1", "html_views"=>"152"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"142"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"117"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"22", "xml_views"=>"1", "html_views"=>"104"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"42", "xml_views"=>"0", "html_views"=>"141"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"82"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"96"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"84"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"43"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"2", "html_views"=>"99"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"96"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"41"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"78"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"57"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"47"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"47"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"105"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"109"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"71"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"3", "html_views"=>"32"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"47"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"3", "html_views"=>"40"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"4", "html_views"=>"31"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"2", "html_views"=>"24"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"33"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"17", "xml_views"=>"6", "html_views"=>"18"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"4", "html_views"=>"12"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"31"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"2", "html_views"=>"22"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"23"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/747820"], "description"=>"<p>Oligonucleotide primers and probes used in this study.</p>", "links"=>[], "tags"=>["primers", "probes"], "article_id"=>418187, "categories"=>["Microbiology", "Infectious Diseases"], "users"=>["Kate Reddington", "Justin O'Grady", "Siobhan Dorai-Raj", "Stefan Niemann", "Dick van Soolingen", "Thomas Barry"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0023481.t001", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Oligonucleotide_primers_and_probes_used_in_this_study_/418187", "title"=>"Oligonucleotide primers and probes used in this study.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2011-08-09 02:16:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/377654", "https://ndownloader.figshare.com/files/377700"], "description"=>"<div><h3>Background</h3><p>Tuberculosis (TB) is the leading cause of death worldwide from a single infectious agent. An ability to detect the <em>Mycobacterium tuberculosis</em> complex (MTC) in clinical material while simultaneously differentiating its members is considered important. This allows for the gathering of epidemiological information pertaining to the prevalence, transmission and geographical distribution of the MTC, including those MTC members associated with zoonotic TB infection in humans. Also differentiating between members of the MTC provides the clinician with inherent MTC specific drug susceptibility profiles to guide appropriate chemotherapy.</p> <h3>Methodology/Principal Findings</h3><p>The aim of this study was to develop a multiplex real-time PCR assay using novel molecular targets to identify and differentiate between the phylogenetically closely related <em>M. bovis</em>, <em>M. bovis</em> BCG and <em>M. caprae</em>. The <em>lpqT</em> gene was explored for the collective identification of <em>M. bovis</em>, <em>M. bovis</em> BCG and <em>M. caprae</em>, the <em>lepA</em> gene was targeted for the specific identification of <em>M. caprae</em> and a Region of Difference 1 (RD1) assay was incorporated in the test to differentiate <em>M. bovis</em> BCG. The multiplex real-time PCR assay was evaluated on 133 bacterial strains and was determined to be 100% specific for the members of the MTC targeted.</p> <h3>Conclusions/Significance</h3><p>The multiplex real-time PCR assay developed in this study is the first assay described for the identification and simultaneous differentiation of <em>M. bovis</em>, <em>M. bovis</em> BCG and <em>M. caprae</em> in one internally controlled reaction. Future validation of this multiplex assay should demonstrate its potential in the rapid and accurate diagnosis of TB caused by these three mycobacteria. Furthermore, the developed assay may be used in conjunction with a recently described multiplex real-time PCR assay for identification of the MTC and simultaneous differentiation of <em>M. tuberculosis</em>, <em>M. canettii</em> resulting in an ability to differentiate five of the eight members of the MTC.</p> </div>", "links"=>[], "tags"=>["multiplex", "Real-time", "pcr", "mycobacteria", "zoonotic", "tuberculosis"], "article_id"=>134630, "categories"=>["Cancer", "Microbiology"], "users"=>["Kate Reddington", "Justin O'Grady", "Siobhan Dorai-Raj", "Stefan Niemann", "Dick van Soolingen", "Thomas Barry"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0023481.s001", "https://dx.doi.org/10.1371/journal.pone.0023481.s002"], "stats"=>{"downloads"=>32, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/A_Novel_Multiplex_Real_Time_PCR_for_the_Identification_of_Mycobacteria_Associated_with_Zoonotic_Tuberculosis/134630", "title"=>"A Novel Multiplex Real-Time PCR for the Identification of Mycobacteria Associated with Zoonotic Tuberculosis", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2011-08-09 01:17:10"}
  • {"files"=>["https://ndownloader.figshare.com/files/747811"], "description"=>"<p>Result interpretation table.</p>", "links"=>[], "tags"=>["Infectious diseases", "microbiology"], "article_id"=>418179, "categories"=>["Microbiology", "Infectious Diseases"], "users"=>["Kate Reddington", "Justin O'Grady", "Siobhan Dorai-Raj", "Stefan Niemann", "Dick van Soolingen", "Thomas Barry"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0023481.t002", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Result_interpretation_table_/418179", "title"=>"Result interpretation table.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2011-08-09 02:16:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/747747"], "description"=>"<p><a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0023481#pone-0023481-g001\" target=\"_blank\">Figure 1 A</a>. Real-time amplification curves for <i>M. caprae</i> (circle) using <i>lepA</i> gene in Cyan500 channel (450–500). <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0023481#pone-0023481-g001\" target=\"_blank\">Figure 1 B</a> Amplification curves for <i>M. caprae</i> (circle), <i>M. bovis</i> (triangles) and <i>M. bovis BCG</i> (diamonds) using the <i>lpqT</i> gene in FAM channel (483–533). <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0023481#pone-0023481-g001\" target=\"_blank\">Figure 1 C</a> Amplification curves for all members of the MTC with the exception of <i>M. bovis</i> BCG (diamonds) and <i>M. microti</i> in the HEX channel (523–568). <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0023481#pone-0023481-g001\" target=\"_blank\">Figure 1 D</a> Amplification curves for IAC in Cy5 channel (615–670) with the no template control highlighted with stars through amplification curve.</p>", "links"=>[], "tags"=>["curves", "multiplex", "Real-time", "pcr"], "article_id"=>418113, "categories"=>["Microbiology", "Infectious Diseases"], "users"=>["Kate Reddington", "Justin O'Grady", "Siobhan Dorai-Raj", "Stefan Niemann", "Dick van Soolingen", "Thomas Barry"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0023481.g001", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Amplification_curves_for_multiplex_real_time_PCR_assay_/418113", "title"=>"Amplification curves for multiplex real-time PCR assay.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-08-09 02:15:13"}

PMC Usage Stats | Further Information

  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"23", "year"=>"2011", "pdf"=>"16", "unique-ip"=>"22", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"77", "unique-ip"=>"70", "pdf"=>"75", "year"=>"2011", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"76", "year"=>"2011", "pdf"=>"62", "unique-ip"=>"70", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"71", "unique-ip"=>"71", "pdf"=>"44", "year"=>"2011", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"9"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"45", "year"=>"2011", "pdf"=>"46", "unique-ip"=>"38", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"35", "unique-ip"=>"42", "pdf"=>"36", "year"=>"2012", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"43", "year"=>"2012", "pdf"=>"29", "unique-ip"=>"42", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"57", "unique-ip"=>"58", "pdf"=>"40", "year"=>"2012", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"3"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"29", "year"=>"2012", "pdf"=>"23", "unique-ip"=>"30", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"30", "unique-ip"=>"33", "pdf"=>"25", "year"=>"2012", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"21", "year"=>"2012", "pdf"=>"12", "unique-ip"=>"20", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"37", "full-text"=>"39", "pdf"=>"24", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"27", "full-text"=>"30", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"18", "full-text"=>"16", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"29", "full-text"=>"31", "pdf"=>"22", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"18", "full-text"=>"16", "pdf"=>"18", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"27", "full-text"=>"30", "pdf"=>"19", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"31", "full-text"=>"33", "pdf"=>"19", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"27", "full-text"=>"27", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"27", "full-text"=>"26", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"29", "full-text"=>"31", "pdf"=>"23", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"28", "full-text"=>"28", "pdf"=>"20", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"17", "full-text"=>"12", "pdf"=>"9", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"19", "full-text"=>"15", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"23", "full-text"=>"19", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"23", "full-text"=>"24", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"22", "full-text"=>"22", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"19", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"26", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"25", "full-text"=>"28", "pdf"=>"14", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"18", "full-text"=>"17", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"23", "full-text"=>"21", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"19", "full-text"=>"18", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"16", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"10", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"26", "full-text"=>"19", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"4", "cited-by"=>"2", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"17", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"23", "full-text"=>"16", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"1", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"18", "full-text"=>"24", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"22", "full-text"=>"34", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"17", "full-text"=>"11", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"16", "full-text"=>"18", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"18", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"8", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"18", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"13", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"16", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"18", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"17", "full-text"=>"29", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"19", "full-text"=>"21", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"16", "full-text"=>"17", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"18", "full-text"=>"18", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"29", "full-text"=>"32", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"19", "full-text"=>"21", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"19", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"18", "full-text"=>"25", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2011-01-01T00:00:00Z", "end_date"=>"2011-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[295, 553, 688, 802, 914, 1017, 1108, 1194, 1278, 1351, 1419, 1500, 1570, 1633, 1712, 1774, 1839, 1903, 1970, 2033, 2101, 2162, 2228, 2291, 2352, 2418, 2486, 2547, 2610, 2680, 2737, 2799, 2859, 2919, 2974, 3029, 3082]}, {"subject_area"=>"/Biology and life sciences/Organisms", "average_usage"=>[316, 571, 699, 812, 915, 1008, 1095, 1180, 1255, 1319, 1393, 1461, 1528, 1595, 1662, 1729, 1802, 1868, 1937, 1997, 2062, 2130, 2192, 2254, 2324, 2386, 2450, 2510, 2579, 2642, 2716, 2778, 2842, 2908, 2979, 3042, 3095]}, {"subject_area"=>"/Biology and life sciences/Veterinary science", "average_usage"=>[362, 655, 777, 893, 988, 1083, 1166, 1276, 1344, 1406, 1458, 1525, 1576, 1632, 1706, 1786, 1854, 1906, 1942, 1993, 2058, 2126, 2184, 2259, 2320, 2380, 2463, 2522, 2570, 2692, 2747, 2804, 2875, 2963, 3005, 3054, 3104]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[303, 571, 709, 823, 937, 1038, 1133, 1216, 1298, 1370, 1447, 1518, 1588, 1656, 1725, 1790, 1856, 1926, 1992, 2054, 2118, 2182, 2242, 2301, 2363, 2425, 2484, 2544, 2602, 2662, 2716, 2776, 2841, 2901, 2960, 3016, 3071]}, {"subject_area"=>"/Medicine and health sciences/Infectious diseases", "average_usage"=>[335, 648, 777, 906, 1018, 1131, 1224, 1307, 1396, 1462, 1541, 1619, 1695, 1764, 1821, 1892, 1959, 2029, 2095, 2155, 2218, 2270, 2329, 2395, 2457, 2528, 2590, 2638, 2708, 2759, 2823, 2882, 2930, 2990, 3041, 3099, 3150]}, {"subject_area"=>"/Medicine and health sciences/Tropical diseases", "average_usage"=>[338, 662, 813, 915, 1030, 1125, 1242, 1315, 1390, 1447, 1525, 1593, 1653, 1750, 1827, 1887, 1972, 2030, 2103, 2153, 2215, 2279, 2336, 2397, 2468, 2528, 2590, 2670, 2746, 2820, 2871, 2936, 2986, 3043, 3080, 3133, 3170]}]}
Loading … Spinner
There are currently no alerts