Insights into Eyestalk Ablation Mechanism to Induce Ovarian Maturation in the Black Tiger Shrimp
Publication Date
September 07, 2011
Journal
PLOS ONE
Authors
Umaporn Uawisetwathana, Rungnapa Leelatanawit, Amornpan Klanchui, Juthatip Prommoon, et al
Volume
6
Issue
9
Pages
e24427
DOI
https://dx.plos.org/10.1371/journal.pone.0024427
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0024427
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/21915325
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3168472
Europe PMC
http://europepmc.org/abstract/MED/21915325
Web of Science
000294802500043
Scopus
80052552082
Mendeley
http://www.mendeley.com/research/insights-eyestalk-ablation-mechanism-induce-ovarian-maturation-black-tiger-shrimp
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Mendeley | Further Information

{"title"=>"Insights into eyestalk ablation mechanism to induce ovarian maturation in the black tiger shrimp", "type"=>"journal", "authors"=>[{"first_name"=>"Umaporn", "last_name"=>"Uawisetwathana", "scopus_author_id"=>"25029307400"}, {"first_name"=>"Rungnapa", "last_name"=>"Leelatanawit", "scopus_author_id"=>"6504471066"}, {"first_name"=>"Amornpan", "last_name"=>"Klanchui", "scopus_author_id"=>"49961753600"}, {"first_name"=>"Juthatip", "last_name"=>"Prommoon", "scopus_author_id"=>"49962091900"}, {"first_name"=>"Sirawut", "last_name"=>"Klinbunga", "scopus_author_id"=>"6603701759"}, {"first_name"=>"Nitsara", "last_name"=>"Karoonuthaisiri", "scopus_author_id"=>"24765223200"}], "year"=>2011, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"80052552082", "doi"=>"10.1371/journal.pone.0024427", "issn"=>"19326203", "pui"=>"362511553", "isbn"=>"1932-6203 (Electronic)\r1932-6203 (Linking)", "pmid"=>"21915325", "scopus"=>"2-s2.0-80052552082"}, "id"=>"423aeccd-ef81-3441-a1f8-130f94a6c642", "abstract"=>"<p>Eyestalk ablation is commonly practiced in crustacean to induce ovarian maturation in captivity. The molecular mechanism of the ablation has not been well understood, preventing a search for alternative measures to induce ovarian maturation in aquaculture. This is the first study to employ cDNA microarray to examine effects of eyestalk ablation at the transcriptomic level and pathway mapping analysis to identify potentially affected biological pathways in the black tiger shrimp (<italic>Penaeus monodon</italic>). Microarray analysis comparing between gene expression levels of ovaries from eyestalk-intact and eyestalk-ablated brooders revealed 682 differentially expressed transcripts. Based on Hierarchical clustering of gene expression patterns, Gene Ontology annotation, and relevant functions of these differentially expressed genes, several gene groups were further examined by pathway mapping analysis. Reverse-transcriptase quantitative PCR analysis for some representative transcripts confirmed microarray data. Known reproductive genes involved in vitellogenesis were dramatically increased during the ablation. Besides these transcripts expected to be induced by the ablation, transcripts whose functions involved in electron transfer mechanism, immune responses and calcium signal transduction were significantly altered following the ablation. Pathway mapping analysis revealed that the activation of gonadotropin-releasing hormone signaling, calcium signaling, and progesterone-mediated oocyte maturation pathways were putatively crucial to ovarian maturation induced by the ablation. These findings shed light on several possible molecular mechanisms of the eyestalk ablation effect and allow more focused investigation for an ultimate goal of finding alternative methods to replace the undesirable practice of the eyestalk ablation in the future.</p>", "link"=>"http://www.mendeley.com/research/insights-eyestalk-ablation-mechanism-induce-ovarian-maturation-black-tiger-shrimp", "reader_count"=>70, "reader_count_by_academic_status"=>{"Unspecified"=>5, "Professor > Associate Professor"=>2, "Librarian"=>1, "Researcher"=>11, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>11, "Student > Postgraduate"=>5, "Student > Master"=>16, "Other"=>2, "Student > Bachelor"=>5, "Lecturer"=>3, "Professor"=>6}, "reader_count_by_user_role"=>{"Unspecified"=>5, "Professor > Associate Professor"=>2, "Librarian"=>1, "Researcher"=>11, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>11, "Student > Postgraduate"=>5, "Student > Master"=>16, "Other"=>2, "Student > Bachelor"=>5, "Lecturer"=>3, "Professor"=>6}, "reader_count_by_subject_area"=>{"Unspecified"=>7, "Environmental Science"=>4, "Biochemistry, Genetics and Molecular Biology"=>3, "Agricultural and Biological Sciences"=>52, "Neuroscience"=>1, "Veterinary Science and Veterinary Medicine"=>1, "Social Sciences"=>1, "Computer Science"=>1}, "reader_count_by_subdiscipline"=>{"Neuroscience"=>{"Neuroscience"=>1}, "Social Sciences"=>{"Social Sciences"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>52}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Unspecified"=>{"Unspecified"=>7}, "Environmental Science"=>{"Environmental Science"=>4}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "reader_count_by_country"=>{"Mexico"=>2, "Thailand"=>1, "India"=>1, "Indonesia"=>1}, "group_count"=>2}

CrossRef

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/738735"], "description"=>"<p>The relative expression levels of the selected transcripts in ovaries before (D0; <i>n</i> = 5) and after eyestalk ablation of <i>P. monodon</i> broodstock at Days 1, 4, and 7 (D1, D4, and D7, respectively; <i>n</i> = 5 each) were measured using RT-qPCR to confirm microarray results and identify other genes potentially involved in ovarian maturation via eyestalk ablation. A total of 12 transcripts were selected to represent three different expression patterns: (A) Late induced, (B) Repressed, and (C) Early induced groups. Different letters above each bar indicate significant difference (<i>P<0.05</i>; Tukey test).</p>", "links"=>[], "tags"=>["quantitative", "pcr"], "article_id"=>409107, "categories"=>["Biotechnology", "Chemistry", "Inorganic Chemistry", "Biochemistry", "Physiology", "Biological Sciences", "Genetics"], "users"=>["Umaporn Uawisetwathana", "Rungnapa Leelatanawit", "Amornpan Klanchui", "Juthatip Prommoon", "Sirawut Klinbunga", "Nitsara Karoonuthaisiri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0024427.g007", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Reverse_transcriptase_quantitative_PCR_RT_qPCR_analysis_/409107", "title"=>"Reverse-transcriptase quantitative PCR (RT-qPCR) analysis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 15:03:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/738499"], "description"=>"<p>The gonadotropin-releasing hormone (GnRH) signaling pathway, calcium signaling pathway, and progesterone-mediated oocyte maturation were identified as putative affected pathways containing early induced genes after the eyestalk ablation. The lighter red boxes represent the genes whose expression levels were induced more than 2 fold, whereas the dark red boxes represent the genes whose expression levels were induced less than 2 fold at Day 1 after the ablation. Abbreviation: GnRHR: gonadotropin-releasing hormone receptor; G q/11: guanine nucleotide-binding protein G(q) subunit alpha; PLC beta: phospholipase C beta; InsP3: inositol-1,4,5-triphosphate; IP3R: inositol-1,4,5-triphosphate receptor; CALR: calreticulin; ER: endoplasmic reticulum; Ca2+: Calcium ion; CaM: calmodulin; CaMK: calmodulin kinase; CaN: calcineurinB; PMCA: Ca2+ transporting ATPase; GPCR?: G protein-coupled receptor; G: guanine nucleotide-binding protein G(i) subunit alpha; AC: adenylate cyclase; PKA: cAMP-dependent protein kinase; Cdc25: cell division cycle25; Cdc2: cell division cycle2; CycB: cyclinB; MAPK: mitogen-activated protein kinase; Rsk1/2: p90 ribosomal S6 kinase; Myt1: membrane-associated tryrosine and threonine-specific cdc2 inhibitor kinase.</p>", "links"=>[], "tags"=>["pathways", "affected", "eyestalk"], "article_id"=>408865, "categories"=>["Biotechnology", "Chemistry", "Inorganic Chemistry", "Biochemistry", "Physiology", "Biological Sciences", "Genetics"], "users"=>["Umaporn Uawisetwathana", "Rungnapa Leelatanawit", "Amornpan Klanchui", "Juthatip Prommoon", "Sirawut Klinbunga", "Nitsara Karoonuthaisiri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0024427.g005", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Putative_pathways_affected_by_the_eyestalk_ablation_/408865", "title"=>"Putative pathways affected by the eyestalk ablation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 15:02:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/739032"], "description"=>"<p>Primers used in Reverse-transcriptase Quantitative PCR (RT-qPCR) analysis.</p>", "links"=>[], "tags"=>["reverse-transcriptase", "quantitative", "pcr"], "article_id"=>409404, "categories"=>["Biotechnology", "Chemistry", "Inorganic Chemistry", "Biochemistry", "Physiology", "Biological Sciences", "Genetics"], "users"=>["Umaporn Uawisetwathana", "Rungnapa Leelatanawit", "Amornpan Klanchui", "Juthatip Prommoon", "Sirawut Klinbunga", "Nitsara Karoonuthaisiri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0024427.t004", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Primers_used_in_Reverse_transcriptase_Quantitative_PCR_RT_qPCR_analysis_/409404", "title"=>"Primers used in Reverse-transcriptase Quantitative PCR (RT-qPCR) analysis.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-20 15:05:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/738322"], "description"=>"<p>Pie diagrams of the percentage distribution of sequences among the three principal GO categories were shown for (A) all 5,568 features on UniShrimpChip and (B) the 682 differentially expressed transcripts. Bar graph showed comparison between all features on UniShrimpChip (darker colored bar) and the differentially expressed transcripts (lighter colored bar) in each GO sub-category: (C) biological processes, (D) cellular components and (E) molecular functions.</p>", "links"=>[], "tags"=>["ontology"], "article_id"=>408695, "categories"=>["Biotechnology", "Chemistry", "Inorganic Chemistry", "Biochemistry", "Physiology", "Biological Sciences", "Genetics"], "users"=>["Umaporn Uawisetwathana", "Rungnapa Leelatanawit", "Amornpan Klanchui", "Juthatip Prommoon", "Sirawut Klinbunga", "Nitsara Karoonuthaisiri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0024427.g003", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Gene_Ontology_GO_annotation_/408695", "title"=>"Gene Ontology (GO) annotation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 15:01:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/738059"], "description"=>"<p>(A) Eyestalk ablation experiment was conducted over 7 days. Ovary samples were collected before eyestalk ablation (D0) and after eyestalk ablation at Days 1, 4 and 7 (D1, D4, and D7, respectively). (B) Examples of representative shrimp (upper panel) and their ovaries (lower panel) from each time point.</p>", "links"=>[], "tags"=>["eyestalk", "ablation", "physiology"], "article_id"=>408429, "categories"=>["Biotechnology", "Chemistry", "Inorganic Chemistry", "Biochemistry", "Physiology", "Biological Sciences", "Genetics"], "users"=>["Umaporn Uawisetwathana", "Rungnapa Leelatanawit", "Amornpan Klanchui", "Juthatip Prommoon", "Sirawut Klinbunga", "Nitsara Karoonuthaisiri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0024427.g001", "stats"=>{"downloads"=>15, "page_views"=>366, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Diagram_of_the_eyestalk_ablation_experiment_and_physiology_of_shrimp_and_ovary_/408429", "title"=>"Diagram of the eyestalk ablation experiment and physiology of shrimp and ovary.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 15:00:22"}
  • {"files"=>["https://ndownloader.figshare.com/files/738415"], "description"=>"<p>A total of 117 pathways were retrieved from the mapping analysis of the 682 differentially expressed transcripts.</p>", "links"=>[], "tags"=>["genes", "assigned", "pathways", "kegg"], "article_id"=>408784, "categories"=>["Biotechnology", "Chemistry", "Inorganic Chemistry", "Biochemistry", "Physiology", "Biological Sciences", "Genetics"], "users"=>["Umaporn Uawisetwathana", "Rungnapa Leelatanawit", "Amornpan Klanchui", "Juthatip Prommoon", "Sirawut Klinbunga", "Nitsara Karoonuthaisiri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0024427.g004", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Distribution_of_number_of_genes_assigned_to_predicted_pathways_from_the_KEGG_database_/408784", "title"=>"Distribution of number of genes assigned to predicted pathways from the KEGG database.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 15:02:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/738892"], "description"=>"<p>*Gonadosomatic Index (GSI) is calculated as a percentage of ovary weight by total body weight.</p><p>Different superscript letters indicate significant differences between groups (<i>p-</i>value<0.05, <i>Tukey</i> test).</p>", "links"=>[], "tags"=>["samples"], "article_id"=>409266, "categories"=>["Biotechnology", "Chemistry", "Inorganic Chemistry", "Biochemistry", "Physiology", "Biological Sciences", "Genetics"], "users"=>["Umaporn Uawisetwathana", "Rungnapa Leelatanawit", "Amornpan Klanchui", "Juthatip Prommoon", "Sirawut Klinbunga", "Nitsara Karoonuthaisiri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0024427.t001", "stats"=>{"downloads"=>2, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Summary_of_shrimp_samples_used_in_this_study_/409266", "title"=>"Summary of shrimp samples used in this study.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-20 15:04:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/738963"], "description"=>"<p>*only genes with differential expression level.</p>", "links"=>[], "tags"=>["pathways", "highest", "numbers", "participated"], "article_id"=>409331, "categories"=>["Biotechnology", "Chemistry", "Inorganic Chemistry", "Biochemistry", "Physiology", "Biological Sciences", "Genetics"], "users"=>["Umaporn Uawisetwathana", "Rungnapa Leelatanawit", "Amornpan Klanchui", "Juthatip Prommoon", "Sirawut Klinbunga", "Nitsara Karoonuthaisiri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0024427.t002", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_pathways_with_the_highest_numbers_of_participated_genes_/409331", "title"=>"The pathways with the highest numbers of participated genes.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-20 15:05:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/738190"], "description"=>"<p>Transcript levels in ovaries after eyestalk ablation at Days 1, 4 and 7 (D1, 4 and 7, respectively; Cy5 Red) were compared to those from non-ablated broodstock (D0, pooled from n = 7; Cy3 Green) using cDNA microarray. (A) A total of 2,743 transcripts present in at least 7 of the 8 microarrays with expression differences greater than median values ± SD (∼1.9-fold) in at least one arrays were selected. (B) Hierarchical clustering analysis of the 682 transcripts with expression difference greater than 2-fold change in at least 4 of the 8 microarrays. (C) Clusters I–III exhibit various differentially expressed patterns: Late induced (I), Repressed (II), and Early induced (III) groups. Transcripts in blue letters were further characterized by reverse- transcriptase quantitative PCR (RT-qPCR).</p>", "links"=>[], "tags"=>["cdna"], "article_id"=>408567, "categories"=>["Biotechnology", "Chemistry", "Inorganic Chemistry", "Biochemistry", "Physiology", "Biological Sciences", "Genetics"], "users"=>["Umaporn Uawisetwathana", "Rungnapa Leelatanawit", "Amornpan Klanchui", "Juthatip Prommoon", "Sirawut Klinbunga", "Nitsara Karoonuthaisiri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0024427.g002", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Gene_expression_analysis_by_cDNA_microarray_/408567", "title"=>"Gene expression analysis by cDNA microarray.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 15:01:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/738928"], "description"=>"<p>List of genes participated in the putative pathways analyzed by KASS.</p>", "links"=>[], "tags"=>["genes", "participated", "putative", "pathways"], "article_id"=>409298, "categories"=>["Biotechnology", "Chemistry", "Inorganic Chemistry", "Biochemistry", "Physiology", "Biological Sciences", "Genetics"], "users"=>["Umaporn Uawisetwathana", "Rungnapa Leelatanawit", "Amornpan Klanchui", "Juthatip Prommoon", "Sirawut Klinbunga", "Nitsara Karoonuthaisiri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0024427.t003", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_List_of_genes_participated_in_the_putative_pathways_analyzed_by_KASS_/409298", "title"=>"List of genes participated in the putative pathways analyzed by KASS.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-20 15:04:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/738998"], "description"=>"<p>*Gonadosomatic Index (GSI) = % gonad weight/body weight.</p>", "links"=>[], "tags"=>["microarray"], "article_id"=>409372, "categories"=>["Biotechnology", "Chemistry", "Inorganic Chemistry", "Biochemistry", "Physiology", "Biological Sciences", "Genetics"], "users"=>["Umaporn Uawisetwathana", "Rungnapa Leelatanawit", "Amornpan Klanchui", "Juthatip Prommoon", "Sirawut Klinbunga", "Nitsara Karoonuthaisiri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0024427.t005", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Summary_of_microarray_experiment_/409372", "title"=>"Summary of microarray experiment.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-20 15:05:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/738654"], "description"=>"<p>Eyestalk ablation releases gonad- or vitellogenesis- inhibiting hormones (GIH or VIH) allowing a synthesis of yolk protein. GnRH activates the calcium signaling pathway resulting in increased levels of intracellular calcium ion through the GnRH signaling and calcium signaling pathways, which subsequently activates folliculogenesis, estradiol and progesterone production in ovary. Progesterone may then induce progesterone-mediated oocyte maturation process from oocyte stage 1 to 4; Oc1–4. “XX” indicates that this type of cell is not known in shrimp but it is pituitary gonadotrope cell in other organisms. Abbreviation: GnRH: gonadotropin-releasing hormone receptor; GIH: gonad inhibiting hormones; VIH: vitellogenesis inhibiting hormones; GV: germinal vesicle; GVBD: germinal vesicle breakdown.</p>", "links"=>[], "tags"=>["induce", "ovarian", "maturation", "eyestalk", "ablation"], "article_id"=>409021, "categories"=>["Biotechnology", "Chemistry", "Inorganic Chemistry", "Biochemistry", "Physiology", "Biological Sciences", "Genetics"], "users"=>["Umaporn Uawisetwathana", "Rungnapa Leelatanawit", "Amornpan Klanchui", "Juthatip Prommoon", "Sirawut Klinbunga", "Nitsara Karoonuthaisiri"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0024427.g006", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_proposed_scheme_to_induce_ovarian_maturation_by_eyestalk_ablation_in_P_monodon_/409021", "title"=>"The proposed scheme to induce ovarian maturation by eyestalk ablation in <i>P. monodon</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 15:03:28"}

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