Glucose-6-Phosphate Dehydrogenase Protects Escherichia coli from Tellurite-Mediated Oxidative Stress
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{"title"=>"Glucose-6-phosphate dehydrogenase protects escherichia coli from tellurite-mediated oxidative stress", "type"=>"journal", "authors"=>[{"first_name"=>"Juan M.", "last_name"=>"Sandoval", "scopus_author_id"=>"8839636300"}, {"first_name"=>"Felipe A.", "last_name"=>"Arenas", "scopus_author_id"=>"24173344200"}, {"first_name"=>"Claudio C.", "last_name"=>"Vásquez", "scopus_author_id"=>"7006212827"}], "year"=>2011, "source"=>"PLoS ONE", "identifiers"=>{"isbn"=>"1932-6203 (Electronic) 1932-6203 (Linking)", "pmid"=>"21984934", "doi"=>"10.1371/journal.pone.0025573", "pui"=>"362706612", "issn"=>"19326203", "sgr"=>"80053588915", "scopus"=>"2-s2.0-80053588915"}, "id"=>"7054b857-bc73-34f1-9827-1c7d287d0af6", "abstract"=>"The tellurium oxyanion tellurite induces oxidative stress in most microorganisms. In Escherichia coli, tellurite exposure results in high levels of oxidized proteins and membrane lipid peroxides, inactivation of oxidation-sensitive enzymes and reduced glutathione content. In this work, we show that tellurite-exposed E. coli exhibits transcriptional activation of the zwf gene, encoding glucose 6-phosphate dehydrogenase (G6PDH), which in turn results in augmented synthesis of reduced nicotinamide adenine dinucleotide phosphate (NADPH). Increased zwf transcription under tellurite stress results mainly from reactive oxygen species (ROS) generation and not from a depletion of cellular glutathione. In addition, the observed increase of G6PDH activity was paralleled by accumulation of glucose-6-phosphate (G6P), suggesting a metabolic flux shift toward the pentose phosphate shunt. Upon zwf overexpression, bacterial cells also show increased levels of antioxidant molecules (NADPH, GSH), better-protected oxidation-sensitive enzymes and decreased amounts of oxidized proteins and membrane lipids. These results suggest that by increasing NADPH content, G6PDH plays an important role in E. coli survival under tellurite stress.", "link"=>"http://www.mendeley.com/research/glucose6phosphate-dehydrogenase-protects-escherichia-coli-telluritemediated-oxidative-stress", "reader_count"=>56, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>3, "Student > Doctoral Student"=>2, "Researcher"=>12, "Student > Ph. D. Student"=>14, "Student > Postgraduate"=>1, "Other"=>3, "Student > Master"=>9, "Student > Bachelor"=>6, "Lecturer"=>1, "Professor"=>5}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>3, "Student > Doctoral Student"=>2, "Researcher"=>12, "Student > Ph. D. Student"=>14, "Student > Postgraduate"=>1, "Other"=>3, "Student > Master"=>9, "Student > Bachelor"=>6, "Lecturer"=>1, "Professor"=>5}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Biochemistry, Genetics and Molecular Biology"=>10, "Materials Science"=>1, "Agricultural and Biological Sciences"=>36, "Medicine and Dentistry"=>2, "Neuroscience"=>1, "Business, Management and Accounting"=>1, "Computer Science"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Neuroscience"=>{"Neuroscience"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>36}, "Computer Science"=>{"Computer Science"=>1}, "Business, Management and Accounting"=>{"Business, Management and Accounting"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>10}, "Unspecified"=>{"Unspecified"=>3}}, "reader_count_by_country"=>{"Turkey"=>1, "Chile"=>2, "Spain"=>2}, "group_count"=>1}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/729392"], "description"=>"<p>Enzymatic activity (µmol NADPH min<sup>−1</sup> mg prot<sup>−1</sup>) was determined spectrophotometrically at 340 nm as described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025573#s3\" target=\"_blank\">Methods</a>. Values are the mean of 4 independent trials ± SD.</p><p>*P≤0.05 as compared to controls. ND, not detected.</p>", "links"=>[], "tags"=>["induces", "nadph-dependent", "enzymatic", "activities"], "article_id"=>399747, "categories"=>["Molecular Biology", "Biochemistry", "Cell Biology", "Microbiology"], "users"=>["Juan M. Sandoval", "Felipe A. Arenas", "Claudio C. Vásquez"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0025573.t002", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Tellurite_induces_NADPH_dependent_enzymatic_activities_in_E_coli_/399747", "title"=>"Tellurite induces NADPH-dependent enzymatic activities in <i>E. coli</i>.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2011-09-30 02:42:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/729357"], "description"=>"a<p>nmol/mg protein. Values are the mean of 3 independent trials ± SD.</p>*<p>, P≤0.05 as compared to control.</p>", "links"=>[], "tags"=>["toxicant", "nadph"], "article_id"=>399715, "categories"=>["Molecular Biology", "Biochemistry", "Cell Biology", "Microbiology"], "users"=>["Juan M. Sandoval", "Felipe A. Arenas", "Claudio C. Vásquez"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0025573.t004", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_zwf_expression_and_toxicant_exposure_on_NADPH_concentration_/399715", "title"=>"Effect of <i>zwf</i> expression and toxicant exposure on NADPH concentration.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2011-09-30 02:41:55"}
  • {"files"=>["https://ndownloader.figshare.com/files/729270"], "description"=>"<p>ROS content was assessed in the indicated strains by flow cytometry using the oxidation-sensitive probe H<sub>2</sub>DCFDA. Expression of <i>zwf</i> was induced in the presence of L-arabinose (0.2%). Cells were incubated for 30 min in the absence or in the presence of 2 µM tellurite, washed and incubated with 2 mM H<sub>2</sub>DCFDA for 30 min in the dark, washed again and diluted 1∶10 with PBS buffer. Fluorescence intensity was determined using a Becton Dickinson model FacsCanto II equipment equipped with an argon laser (excitation 490 nm, emission 519 nm) <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025573#pone.0025573-Lushchak1\" target=\"_blank\">[45]</a>. The per cent of cell population that was positive for fluorescence is indicated (blue rectangles). A representative dot plot of 3 independent trials is shown. FITC, fluorescence intensity; SSC-A, cell complexity.</p>", "links"=>[], "tags"=>["ros"], "article_id"=>399636, "categories"=>["Molecular Biology", "Biochemistry", "Cell Biology", "Microbiology"], "users"=>["Juan M. Sandoval", "Felipe A. Arenas", "Claudio C. Vásquez"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0025573.g003", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_zwf_expression_on_ROS_content_/399636", "title"=>"Effect of <i>zwf</i> expression on ROS content.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-09-30 02:40:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/729210"], "description"=>"<p>Growth inhibition zones were determined for wild type (BW25113 pBAD), <i>zwf</i>-overexpressing (pBAD-<i>zwf</i>), mutant (BW25113 Δ<i>zwf</i>::<i>kan</i>) and genetically complemented (BW25113 Δ<i>zwf</i>::<i>kan</i> pBAD-<i>zwf</i>) strains as described <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025573#pone.0025573-Herrera1\" target=\"_blank\">[41]</a>. Briefly, cells were grown to OD<sub>600</sub>∼0.5, diluted and spread on LB plates. After air drying, ten microliters of tellurite (40 mM), H<sub>2</sub>O<sub>2</sub> (10 M) or diamide (100 mM) were deposited on sterile disks in the centers of the plates. Results were determined after 24 h. Values are the mean of 4–5 independent trials ± SD.</p>", "links"=>[], "tags"=>["oxidative"], "article_id"=>399567, "categories"=>["Molecular Biology", "Biochemistry", "Cell Biology", "Microbiology"], "users"=>["Juan M. Sandoval", "Felipe A. Arenas", "Claudio C. Vásquez"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0025573.g002", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_zwf_expression_on_the_E_coli_sensitivity_to_oxidative_stress_elicitors_/399567", "title"=>"Effect of <i>zwf</i> expression on the <i>E. coli</i> sensitivity to oxidative stress elicitors.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-09-30 02:39:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/729117"], "description"=>"<p>(A) G6PDH activity was determined spectrophotometrically at 340 nm as described <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025573#pone.0025573-Asnis1\" target=\"_blank\">[49]</a>. (B) Intracellular ROS levels were analyzed using the oxidation-sensitive probe H<sub>2</sub>DCFDA (2′,7′-dichlorofluorescein diacetate) using an Applied Biosystems equipment CytoFluor 4000 Fluorescence Multi-well Plate Reader (excitation 490 nm, emission 519 nm) and normalized to protein concentration. (C) GSH content was assessed as described previously <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025573#pone.0025573-Griffith2\" target=\"_blank\">[56]</a> with modifications. (D) β-galactosidase activity was determined in extracts of the reporter <i>E. coli</i> GC4468 <i>zwf</i>::<i>lacZ</i> strain <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025573#pone.0025573-Semchyshyn1\" target=\"_blank\">[42]</a> by monitoring the hydrolysis of <i>o</i>-nitrophenyl-β-D-galactopyranoside as described <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025573#pone.0025573-ContrerasNdel1\" target=\"_blank\">[43]</a>. (E) Western blotting of G6PDH was analyzed using a specific <i>in-house</i> made antiserum. Band intensities were analyzed using the Gel-Pro Analyzer Program software, version 3.1. Relative expression was referred to that of control cells. The strain used in A–C and E was <i>E. coli</i> BW25113. <i>E. coli</i> cells were left untreated (control, white) or treated with 2 µM tellurite (black), 100 µM menadione (horizontal stripes) or 500 µM diamide (grey) for 30 min. Values are the mean ± SD of 3–4 independent trials. au, arbitrary units.</p>", "links"=>[], "tags"=>["induces", "g6pdh", "ros", "thiol", "depletion"], "article_id"=>399472, "categories"=>["Molecular Biology", "Biochemistry", "Cell Biology", "Microbiology"], "users"=>["Juan M. Sandoval", "Felipe A. Arenas", "Claudio C. Vásquez"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0025573.g001", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Tellurite_induces_G6PDH_because_of_ROS_formation_and_not_thiol_depletion_in_E_coli_/399472", "title"=>"Tellurite induces G6PDH because of ROS formation and not thiol depletion in <i>E. coli</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-09-30 02:37:52"}
  • {"files"=>["https://ndownloader.figshare.com/files/729448"], "description"=>"a<p>NADP(H) (nmol mg prot<sup>−1</sup>) and NAD(H) (mmol mg prot<sup>−1</sup>) concentration in <i>E. coli</i> BW25113 extracts was determined spectrophotometrically at 340 nm as described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025573#s3\" target=\"_blank\">Methods</a>. Values are the mean of 3 independent trials ± SD.</p><p>*P≤0.05 as compared to controls.</p>", "links"=>[], "tags"=>["augmented", "nadph", "levels"], "article_id"=>399809, "categories"=>["Molecular Biology", "Biochemistry", "Cell Biology", "Microbiology"], "users"=>["Juan M. Sandoval", "Felipe A. Arenas", "Claudio C. Vásquez"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0025573.t001", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Tellurite_exposure_results_in_augmented_NADPH_levels_in_E_coli_/399809", "title"=>"Tellurite exposure results in augmented NADPH levels in <i>E. coli</i>.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2011-09-30 02:43:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/729416"], "description"=>"a<p>Intracellular G6P concentration (nmol mg prot<sup>−1</sup>);</p>b<p>Enzymatic activity (µmol NADPH min<sup>−1</sup> mg prot<sup>−1</sup>) was determined spectrophotometrically at 340 nm as described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025573#s3\" target=\"_blank\">Methods</a>. Values are the mean of 3 independent trials ± SD.</p><p>*P≤0.05 as compared to controls.</p>", "links"=>[], "tags"=>["induces", "g6p", "accumulation"], "article_id"=>399777, "categories"=>["Molecular Biology", "Biochemistry", "Cell Biology", "Microbiology"], "users"=>["Juan M. Sandoval", "Felipe A. Arenas", "Claudio C. Vásquez"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0025573.t003", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Tellurite_exposure_induces_G6P_accumulation_in_E_coli_/399777", "title"=>"Tellurite exposure induces G6P accumulation in <i>E. coli</i>.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2011-09-30 02:42:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/368503", "https://ndownloader.figshare.com/files/368525", "https://ndownloader.figshare.com/files/368546", "https://ndownloader.figshare.com/files/368562"], "description"=>"<div><p>The tellurium oxyanion tellurite induces oxidative stress in most microorganisms. In <em>Escherichia coli</em>, tellurite exposure results in high levels of oxidized proteins and membrane lipid peroxides, inactivation of oxidation-sensitive enzymes and reduced glutathione content. In this work, we show that tellurite-exposed <em>E. coli</em> exhibits transcriptional activation of the <em>zwf</em> gene, encoding glucose 6-phosphate dehydrogenase (G6PDH), which in turn results in augmented synthesis of reduced nicotinamide adenine dinucleotide phosphate (NADPH). Increased <em>zwf</em> transcription under tellurite stress results mainly from reactive oxygen species (ROS) generation and not from a depletion of cellular glutathione. In addition, the observed increase of G6PDH activity was paralleled by accumulation of glucose-6-phosphate (G6P), suggesting a metabolic flux shift toward the pentose phosphate shunt. Upon <em>zwf</em> overexpression, bacterial cells also show increased levels of antioxidant molecules (NADPH, GSH), better-protected oxidation-sensitive enzymes and decreased amounts of oxidized proteins and membrane lipids. These results suggest that by increasing NADPH content, G6PDH plays an important role in <em>E. coli</em> survival under tellurite stress.</p> </div>", "links"=>[], "tags"=>["glucose-6-phosphate", "dehydrogenase", "protects", "tellurite-mediated", "oxidative"], "article_id"=>132818, "categories"=>["Molecular Biology", "Biochemistry", "Cell Biology", "Microbiology"], "users"=>["Juan M. Sandoval", "Felipe A. Arenas", "Claudio C. Vásquez"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0025573.s001", "https://dx.doi.org/10.1371/journal.pone.0025573.s002", "https://dx.doi.org/10.1371/journal.pone.0025573.s003", "https://dx.doi.org/10.1371/journal.pone.0025573.s004"], "stats"=>{"downloads"=>13, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Glucose_6_Phosphate_Dehydrogenase_Protects_Escherichia_coli_from_Tellurite_Mediated_Oxidative_Stress/132818", "title"=>"Glucose-6-Phosphate Dehydrogenase Protects <em>Escherichia coli</em> from Tellurite-Mediated Oxidative Stress", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2011-09-30 00:46:58"}

PMC Usage Stats | Further Information

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Relative Metric

{"start_date"=>"2011-01-01T00:00:00Z", "end_date"=>"2011-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Physical sciences/Chemistry", "average_usage"=>[272, 506, 626, 733, 837, 944, 1031, 1116, 1192, 1253, 1324, 1388, 1459, 1528, 1593, 1653, 1718, 1787, 1854, 1916, 1974, 2045, 2111, 2167, 2232, 2297, 2355, 2425, 2489, 2549, 2615, 2675, 2734, 2793, 2851, 2904, 2961]}]}
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