The Transcription Factor Ndt80 Does Not Contribute to Mrr1-, Tac1-, and Upc2-Mediated Fluconazole Resistance in Candida albicans
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"The transcription factor Ndt80 does not contribute to Mrr1-, Tac1-, and Upc2-mediated fluconazole resistance in Candida albicans", "type"=>"journal", "authors"=>[{"first_name"=>"Christoph", "last_name"=>"Sasse", "scopus_author_id"=>"36795460200"}, {"first_name"=>"Rebecca", "last_name"=>"Schillig", "scopus_author_id"=>"49461830700"}, {"first_name"=>"Franziska", "last_name"=>"Dierolf", "scopus_author_id"=>"37113559900"}, {"first_name"=>"Michael", "last_name"=>"Weyler", "scopus_author_id"=>"6507462116"}, {"first_name"=>"Sabrina", "last_name"=>"Schneider", "scopus_author_id"=>"26025767200"}, {"first_name"=>"Selene", "last_name"=>"Mogavero", "scopus_author_id"=>"35102998100"}, {"first_name"=>"P. David", "last_name"=>"Rogers", "scopus_author_id"=>"35595047300"}, {"first_name"=>"Joachim", "last_name"=>"Morschhäuser", "scopus_author_id"=>"7005253431"}], "year"=>2011, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "pui"=>"362635647", "sgr"=>"80053187522", "doi"=>"10.1371/journal.pone.0025623", "scopus"=>"2-s2.0-80053187522", "isbn"=>"1932-6203 (Electronic)\\n1932-6203 (Linking)", "pmid"=>"21980509"}, "id"=>"da21ec78-fa62-3915-9d1a-d96e3206ebcb", "abstract"=>"The pathogenic yeast Candida albicans can develop resistance to the widely used antifungal agent fluconazole, which inhibits ergosterol biosynthesis, by the overexpression of genes encoding multidrug efflux pumps or ergosterol biosynthesis enzymes. Zinc cluster transcription factors play a central role in the transcriptional regulation of drug resistance. Mrr1 regulates the expression of the major facilitator MDR1, Tac1 controls the expression of the ABC transporters CDR1 and CDR2, and Upc2 regulates ergosterol biosynthesis (ERG) genes. Gain-of-function mutations in these transcription factors result in constitutive overexpression of their target genes and are responsible for fluconazole resistance in many clinical C. albicans isolates. The transcription factor Ndt80 contributes to the drug-induced upregulation of CDR1 and ERG genes and also binds to the MDR1 and CDR2 promoters, suggesting that it is an important component of all major transcriptional mechanisms of fluconazole resistance. However, we found that Ndt80 is not required for the induction of MDR1 and CDR2 expression by inducing chemicals. CDR2 was even partially derepressed in ndt80Δ mutants, indicating that Ndt80 is a repressor of CDR2 expression. Hyperactive forms of Mrr1, Tac1, and Upc2 promoted overexpression of MDR1, CDR1/CDR2, and ERG11, respectively, with the same efficiency in the presence and absence of Ndt80. Mrr1- and Tac1-mediated fluconazole resistance was even slightly enhanced in ndt80Δ mutants compared to wild-type cells. These results demonstrate that Ndt80 is dispensable for the constitutive overexpression of Mrr1, Tac1, and Upc2 target genes and the increased fluconazole resistance of strains that have acquired activating mutations in these transcription factors.", "link"=>"http://www.mendeley.com/research/transcription-factor-ndt80-not-contribute-mrr1-tac1-upc2mediated-fluconazole-resistance-candida-albi", "reader_count"=>22, "reader_count_by_academic_status"=>{"Researcher"=>5, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>3, "Student > Master"=>5, "Student > Bachelor"=>6}, "reader_count_by_user_role"=>{"Researcher"=>5, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>3, "Student > Master"=>5, "Student > Bachelor"=>6}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>2, "Medicine and Dentistry"=>3, "Agricultural and Biological Sciences"=>17}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>17}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}}, "reader_count_by_country"=>{"Turkey"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/80053187522"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/80053187522?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=80053187522&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=80053187522&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/80053187522", "dc:identifier"=>"SCOPUS_ID:80053187522", "eid"=>"2-s2.0-80053187522", "dc:title"=>"The transcription factor Ndt80 does not contribute to Mrr1-, Tac1-, and Upc2-mediated fluconazole resistance in Candida albicans", "dc:creator"=>"Sasse C.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"6", "prism:issueIdentifier"=>"9", "prism:pageRange"=>nil, "prism:coverDate"=>"2011-09-27", "prism:coverDisplayDate"=>"27 September 2011", "prism:doi"=>"10.1371/journal.pone.0025623", "citedby-count"=>"37", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Julius-Maximilians-Universität Würzburg", "affiliation-city"=>"Wurzburg", "affiliation-country"=>"Germany"}], "pubmed-id"=>"21980509", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e25623", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0025623", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"9", "year"=>"2011", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"71"}
  • {"month"=>"10", "year"=>"2011", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"94"}
  • {"month"=>"11", "year"=>"2011", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"52"}
  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"24", "xml_views"=>"16", "html_views"=>"27"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"50", "xml_views"=>"41", "html_views"=>"24"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"24", "xml_views"=>"16", "html_views"=>"18"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"27", "xml_views"=>"17", "html_views"=>"22"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"23", "xml_views"=>"13", "html_views"=>"16"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"4", "html_views"=>"19"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"32", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"33", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"35", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"36", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"3", "html_views"=>"13"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"13"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"28"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"22"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"12"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"16"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"17"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"4", "html_views"=>"5"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"24", "xml_views"=>"2", "html_views"=>"15"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"8"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"1"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/732177"], "description"=>"<p>(A) Reporter strains containing wild-type or hyperactive <i>UPC2</i> alleles and expressing <i>GFP</i> under the control of the <i>ERG11</i> promoter were grown to log phase in YPD medium. The mean fluorescence of the cells was determined by flow cytometry. The results obtained with two independently generated reporter strains are shown in each case (means and standard deviations from three experiments). The following strains were used (see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025623#pone.0025623.s001\" target=\"_blank\">Table S1</a>): SCEG2A and -B (wild type, <i>UPC2</i>), SCUPC2R14CG2A and -B (wild type, <i>UPC2</i><sup>G648D</sup>), SCΔ<i>ndt80</i>EG2A and -B (<i>ndt80</i>Δ, <i>UPC2</i>) SCΔ<i>ndt80</i>UPC2R14EG2A and -B (<i>ndt80</i>Δ, <i>UPC2</i><sup>G648D</sup>). The background fluorescence of the parental strains, which do not contain the <i>GFP</i> gene, is indicated by the black part in each column. (B) GFP expression in the same strains was detected by Western immunoblotting with an anti-GFP antibody. (C) MIC of fluconazole for strains containing wild-type <i>UPC2</i> or <i>UPC2</i><sup>G648D</sup> alleles in the indicated genetic backgrounds.</p>", "links"=>[], "tags"=>["promoter", "fluconazole", "strains", "expressing", "hyperactive", "allele", "wild-type"], "article_id"=>402522, "categories"=>["Molecular Biology", "Biological Sciences", "Genetics", "Microbiology"], "users"=>["Christoph Sasse", "Rebecca Schillig", "Franziska Dierolf", "Michael Weyler", "Sabrina Schneider", "Selene Mogavero", "P. David Rogers", "Joachim Morschhäuser"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0025623.g006", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ERG11_promoter_activity_and_fluconazole_resistance_of_strains_expressing_the_hyperactive_UPC2_G648D_allele_in_wild_type_and_ndt80_backgrounds_/402522", "title"=>"<i>ERG11</i> promoter activity and fluconazole resistance of strains expressing the hyperactive <i>UPC2</i><sup>G648D</sup> allele in wild-type and <i>ndt80</i>Δ backgrounds.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-09-27 00:42:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/732098"], "description"=>"<p>(A) Reporter strains containing wild-type or hyperactive <i>TAC1</i> alleles and expressing <i>GFP</i> under the control of the <i>CDR1</i> promoter were grown to log phase in YPD medium. The mean fluorescence of the cells was determined by flow cytometry. The results obtained with two independently generated reporter strains are shown in each case (means and standard deviations from three experiments). The following strains were used (see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025623#pone.0025623.s001\" target=\"_blank\">Table S1</a>): SCCG1A and -B (wild type, <i>TAC1</i>), SCTAC1R34CG1A and -B (wild type, <i>TAC1</i><sup>G980E</sup>), SCΔ<i>ndt80</i>CG1A and -B (<i>ndt80</i>Δ, <i>TAC1</i>), SCΔ<i>ndt80</i>TAC1R34CG1A and -B (<i>ndt80</i>Δ, <i>TAC1</i><sup>G980E</sup>). The background fluorescence of the parental strains, which do not contain the <i>GFP</i> gene, is indicated by the black part in each column. (B) GFP expression in the same strains was detected by Western immunoblotting with an anti-GFP antibody.</p>", "links"=>[], "tags"=>["promoter", "strains", "expressing", "hyperactive", "allele", "wild-type"], "article_id"=>402440, "categories"=>["Molecular Biology", "Biological Sciences", "Genetics", "Microbiology"], "users"=>["Christoph Sasse", "Rebecca Schillig", "Franziska Dierolf", "Michael Weyler", "Sabrina Schneider", "Selene Mogavero", "P. David Rogers", "Joachim Morschhäuser"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0025623.g005", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_CDR1_promoter_activity_in_strains_expressing_the_hyperactive_TAC1_G980E_allele_in_wild_type_and_ndt80_backgrounds_/402440", "title"=>"<i>CDR1</i> promoter activity in strains expressing the hyperactive <i>TAC1</i><sup>G980E</sup> allele in wild-type and <i>ndt80</i>Δ backgrounds.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-09-27 00:40:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/731909"], "description"=>"<p>(A) Strains carrying a P<i><sub>CDR2</sub>-GFP</i> reporter fusion in the indicated genetic backgrounds were grown in the absence (−) or presence (+) of fluphenazine as described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025623#s4\" target=\"_blank\">Materials and methods</a>. The mean fluorescence of the cells was determined by flow cytometry. The results obtained with two independently generated reporter strains are shown in each case (means and standard deviations from three experiments). The following strains were used (see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025623#pone.0025623.s001\" target=\"_blank\">Table S1</a>): SCCG3A and -B (wild type), SCΔ<i>tac1</i>CG3A and -B (<i>tac1</i>Δ), SCΔ<i>ndt80</i>CG3A and -B (<i>ndt80</i>Δ). The background fluorescence of the parental strains, which do not contain the <i>GFP</i> gene, is indicated by the black part in each column. (B) GFP expression in the same strains was detected by Western immunoblotting with an anti-GFP antibody. The two blots show the results with the A and B series of strains.</p>", "links"=>[], "tags"=>["promoter"], "article_id"=>402260, "categories"=>["Molecular Biology", "Biological Sciences", "Genetics", "Microbiology"], "users"=>["Christoph Sasse", "Rebecca Schillig", "Franziska Dierolf", "Michael Weyler", "Sabrina Schneider", "Selene Mogavero", "P. David Rogers", "Joachim Morschhäuser"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0025623.g003", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Inducibility_of_the_CDR2_promoter_in_wild_type_tac1_and_ndt80_strains_/402260", "title"=>"Inducibility of the <i>CDR2</i> promoter in wild-type, <i>tac1</i>Δ, and <i>ndt80</i>Δ strains.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-09-27 00:37:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/731765"], "description"=>"<p>(A) Strains carrying a P<i><sub>MDR1</sub>-GFP</i> reporter fusion in the indicated genetic backgrounds were grown in the absence (−) or presence (+) of benomyl as described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025623#s4\" target=\"_blank\">Materials and methods</a>. The mean fluorescence of the cells was determined by flow cytometry. The results obtained with two independently generated reporter strains are shown in each case (means and standard deviations from three experiments). The following strains were used (see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025623#pone.0025623.s001\" target=\"_blank\">Table S1</a>): SCMG3A and -B (wild type), SCΔ<i>mrr1</i>MG3A and -B (<i>mrr1</i>Δ), SCΔ<i>ndt80</i>MG3A and -B (<i>ndt80</i>Δ). The background fluorescence of the parental strains, which do not contain the <i>GFP</i> gene, is indicated by the black part in each column. (B) GFP expression in the same strains was detected by Western immunoblotting with an anti-GFP antibody. The two blots show the results with the A and B series of strains.</p>", "links"=>[], "tags"=>["promoter"], "article_id"=>402118, "categories"=>["Molecular Biology", "Biological Sciences", "Genetics", "Microbiology"], "users"=>["Christoph Sasse", "Rebecca Schillig", "Franziska Dierolf", "Michael Weyler", "Sabrina Schneider", "Selene Mogavero", "P. David Rogers", "Joachim Morschhäuser"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0025623.g001", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Inducibility_of_the_MDR1_promoter_in_wild_type_mrr1_and_ndt80_strains_/402118", "title"=>"Inducibility of the <i>MDR1</i> promoter in wild-type, <i>mrr1</i>Δ, and <i>ndt80</i>Δ strains.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-09-27 00:35:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/369366", "https://ndownloader.figshare.com/files/369493"], "description"=>"<div><p>The pathogenic yeast <em>Candida albicans</em> can develop resistance to the widely used antifungal agent fluconazole, which inhibits ergosterol biosynthesis, by the overexpression of genes encoding multidrug efflux pumps or ergosterol biosynthesis enzymes. Zinc cluster transcription factors play a central role in the transcriptional regulation of drug resistance. Mrr1 regulates the expression of the major facilitator <em>MDR1</em>, Tac1 controls the expression of the ABC transporters <em>CDR1</em> and <em>CDR2</em>, and Upc2 regulates ergosterol biosynthesis (<em>ERG</em>) genes. Gain-of-function mutations in these transcription factors result in constitutive overexpression of their target genes and are responsible for fluconazole resistance in many clinical <em>C. albicans</em> isolates. The transcription factor Ndt80 contributes to the drug-induced upregulation of <em>CDR1</em> and <em>ERG</em> genes and also binds to the <em>MDR1</em> and <em>CDR2</em> promoters, suggesting that it is an important component of all major transcriptional mechanisms of fluconazole resistance. However, we found that Ndt80 is not required for the induction of <em>MDR1</em> and <em>CDR2</em> expression by inducing chemicals. <em>CDR2</em> was even partially derepressed in <em>ndt80</em>Δ mutants, indicating that Ndt80 is a repressor of <em>CDR2</em> expression. Hyperactive forms of Mrr1, Tac1, and Upc2 promoted overexpression of <em>MDR1</em>, <em>CDR1</em>/<em>CDR2</em>, and <em>ERG11</em>, respectively, with the same efficiency in the presence and absence of Ndt80. Mrr1- and Tac1-mediated fluconazole resistance was even slightly enhanced in <em>ndt80</em>Δ mutants compared to wild-type cells. These results demonstrate that Ndt80 is dispensable for the constitutive overexpression of Mrr1, Tac1, and Upc2 target genes and the increased fluconazole resistance of strains that have acquired activating mutations in these transcription factors.</p> </div>", "links"=>[], "tags"=>["transcription", "ndt80", "upc2-mediated", "fluconazole"], "article_id"=>132980, "categories"=>["Molecular Biology", "Biological Sciences", "Genetics", "Microbiology"], "users"=>["Christoph Sasse", "Rebecca Schillig", "Franziska Dierolf", "Michael Weyler", "Sabrina Schneider", "Selene Mogavero", "P. David Rogers", "Joachim Morschhäuser"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0025623.s001", "https://dx.doi.org/10.1371/journal.pone.0025623.s002"], "stats"=>{"downloads"=>2, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/The_Transcription_Factor_Ndt80_Does_Not_Contribute_to_Mrr1_Tac1_and_Upc2_Mediated_Fluconazole_Resistance_in_Candida_albicans_/132980", "title"=>"The Transcription Factor Ndt80 Does Not Contribute to Mrr1-, Tac1-, and Upc2-Mediated Fluconazole Resistance in <em>Candida albicans</em>", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2011-09-27 00:49:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/731843"], "description"=>"<p>(A) Reporter strains containing wild-type or hyperactive <i>MRR1</i> alleles and expressing <i>GFP</i> under the control of the <i>MDR1</i> promoter were grown to log phase in YPD medium. The mean fluorescence of the cells was determined by flow cytometry. The results obtained with two independently generated reporter strains are shown in each case (means and standard deviations from three experiments). The following strains were used (see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025623#pone.0025623.s001\" target=\"_blank\">Table S1</a>): SCMG3A and -B (wild type, <i>MRR1</i>), SCMRR1R34MG3A and -B (wild type, <i>MRR1</i><sup>P683S</sup>), SCΔ<i>ndt80</i>MG3A and -B (<i>ndt80</i>Δ, <i>MRR1</i>), SCΔ<i>ndt80</i>MRR1R34MG3A and -B (<i>ndt80</i>Δ, <i>MRR1</i><sup>P683S</sup>). The background fluorescence of the parental strains, which do not contain the <i>GFP</i> gene, is indicated by the black part in each column. (B) GFP expression in the same strains was detected by Western immunoblotting with an anti-GFP antibody. (C) MIC of fluconazole for strains containing wild-type <i>MRR1</i> or <i>MRR1</i><sup>P683S</sup> alleles in the indicated genetic backgrounds. *, reduced growth was already observed at 6.25 µg/ml fluconazole.</p>", "links"=>[], "tags"=>["promoter", "fluconazole", "strains", "expressing", "hyperactive", "allele", "wild-type"], "article_id"=>402181, "categories"=>["Molecular Biology", "Biological Sciences", "Genetics", "Microbiology"], "users"=>["Christoph Sasse", "Rebecca Schillig", "Franziska Dierolf", "Michael Weyler", "Sabrina Schneider", "Selene Mogavero", "P. David Rogers", "Joachim Morschhäuser"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0025623.g002", "stats"=>{"downloads"=>2, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_MDR1_promoter_activity_and_fluconazole_resistance_of_strains_expressing_the_hyperactive_MRR1_P683S_allele_in_wild_type_and_ndt80_backgrounds_/402181", "title"=>"<i>MDR1</i> promoter activity and fluconazole resistance of strains expressing the hyperactive <i>MRR1</i><sup>P683S</sup> allele in wild-type and <i>ndt80</i>Δ backgrounds.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-09-27 00:36:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/731997"], "description"=>"<p>(A) Reporter strains containing wild-type or hyperactive <i>TAC1</i> alleles and expressing <i>GFP</i> under the control of the <i>CDR2</i> promoter were grown to log phase in YPD medium. The mean fluorescence of the cells was determined by flow cytometry. The results obtained with two independently generated reporter strains are shown in each case (means and standard deviations from three experiments). The following strains were used (see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025623#pone.0025623.s001\" target=\"_blank\">Table S1</a>): SCCG3A and -B (wild type, <i>TAC1</i>), SCTAC1R34CG3A and -B (wild type, <i>TAC1</i><sup>G980E</sup>), SCΔ<i>ndt80</i>CG3A and -B (<i>ndt80</i>Δ, <i>TAC1</i>), SCΔ<i>ndt80</i>TAC1R34CG3A and -B (<i>ndt80</i>Δ, <i>TAC1</i><sup>G980E</sup>). The background fluorescence of the parental strains, which do not contain the <i>GFP</i> gene, is indicated by the black part in each column. (B) GFP expression in the same strains was detected by Western immunoblotting with an anti-GFP antibody. (C) MIC of fluconazole for strains containing wild-type <i>TAC1</i> or <i>TAC1</i><sup>G980E</sup> alleles in the indicated genetic backgrounds. *, reduced growth was already observed at 3.13 µg/ml fluconazole.</p>", "links"=>[], "tags"=>["promoter", "fluconazole", "strains", "expressing", "hyperactive", "allele", "wild-type"], "article_id"=>402340, "categories"=>["Molecular Biology", "Biological Sciences", "Genetics", "Microbiology"], "users"=>["Christoph Sasse", "Rebecca Schillig", "Franziska Dierolf", "Michael Weyler", "Sabrina Schneider", "Selene Mogavero", "P. David Rogers", "Joachim Morschhäuser"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0025623.g004", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_CDR2_promoter_activity_and_fluconazole_resistance_of_strains_expressing_the_hyperactive_TAC1_G980E_allele_in_wild_type_and_ndt80_backgrounds_/402340", "title"=>"<i>CDR2</i> promoter activity and fluconazole resistance of strains expressing the hyperactive <i>TAC1</i><sup>G980E</sup> allele in wild-type and <i>ndt80</i>Δ backgrounds.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-09-27 00:39:00"}

PMC Usage Stats | Further Information

  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"32", "year"=>"2011", "pdf"=>"22", "unique-ip"=>"33", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"33", "unique-ip"=>"28", "pdf"=>"19", "year"=>"2011", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"19", "year"=>"2011", "pdf"=>"17", "unique-ip"=>"21", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"10", "unique-ip"=>"11", "pdf"=>"8", "year"=>"2012", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"25", "year"=>"2012", "pdf"=>"23", "unique-ip"=>"19", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"9"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"18", "unique-ip"=>"16", "pdf"=>"12", "year"=>"2012", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"15", "year"=>"2012", "pdf"=>"13", "unique-ip"=>"17", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"1", "abstract"=>"1", "full-text"=>"24", "unique-ip"=>"20", "pdf"=>"19", "year"=>"2012", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"25", "year"=>"2012", "pdf"=>"9", "unique-ip"=>"13", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"14", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"18", "full-text"=>"19", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"19", "full-text"=>"14", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"26", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"28", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"22", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"17", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"15", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"26", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"4", "cited-by"=>"1", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"21", "full-text"=>"19", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"1", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"15", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"21", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"16", "full-text"=>"10", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"6", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"10", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"12", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"19", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}

Relative Metric

{"start_date"=>"2011-01-01T00:00:00Z", "end_date"=>"2011-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[304, 568, 702, 818, 927, 1027, 1118, 1206, 1285, 1357, 1427, 1500, 1564, 1636, 1705, 1773, 1840, 1909, 1974, 2039, 2106, 2170, 2234, 2296, 2358, 2423, 2484, 2546, 2606, 2673, 2734, 2795, 2857, 2921, 2984, 3046, 3100]}, {"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[290, 559, 698, 813, 927, 1028, 1121, 1206, 1289, 1360, 1433, 1501, 1569, 1637, 1707, 1774, 1841, 1911, 1974, 2038, 2103, 2161, 2219, 2280, 2345, 2406, 2466, 2529, 2592, 2654, 2713, 2772, 2835, 2893, 2954, 3020, 3071]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[289, 559, 699, 817, 931, 1031, 1127, 1214, 1297, 1370, 1444, 1515, 1584, 1656, 1726, 1797, 1862, 1930, 1996, 2061, 2125, 2190, 2250, 2311, 2373, 2435, 2496, 2563, 2630, 2692, 2760, 2824, 2898, 2960, 3019, 3089, 3143]}, {"subject_area"=>"/Biology and life sciences/Microbiology", "average_usage"=>[313, 585, 721, 833, 946, 1049, 1147, 1230, 1307, 1383, 1462, 1528, 1592, 1667, 1726, 1794, 1857, 1918, 1993, 2070, 2137, 2194, 2258, 2314, 2370, 2432, 2497, 2555, 2619, 2676, 2741, 2801, 2861, 2914, 2971, 3017, 3072]}, {"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[295, 553, 688, 802, 914, 1017, 1108, 1194, 1278, 1351, 1419, 1500, 1570, 1633, 1712, 1774, 1839, 1903, 1970, 2033, 2101, 2162, 2228, 2291, 2352, 2418, 2486, 2547, 2610, 2680, 2737, 2799, 2859, 2919, 2974, 3029, 3082]}, {"subject_area"=>"/Biology and life sciences/Mycology", "average_usage"=>[330, 564, 723, 851, 962, 1064, 1145, 1232, 1331, 1386, 1446, 1540, 1589, 1706, 1779, 1864, 1945, 2015, 2066, 2138, 2184, 2251, 2303, 2352, 2438, 2485, 2576, 2685, 2798, 2848, 2905, 2960, 3040, 3125, 3181, 3258, 3319]}, {"subject_area"=>"/Biology and life sciences/Organisms", "average_usage"=>[316, 571, 699, 812, 915, 1008, 1095, 1180, 1255, 1319, 1393, 1461, 1528, 1595, 1662, 1729, 1802, 1868, 1937, 1997, 2062, 2130, 2192, 2254, 2324, 2386, 2450, 2510, 2579, 2642, 2716, 2778, 2842, 2908, 2979, 3042, 3095]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[303, 571, 709, 823, 937, 1038, 1133, 1216, 1298, 1370, 1447, 1518, 1588, 1656, 1725, 1790, 1856, 1926, 1992, 2054, 2118, 2182, 2242, 2301, 2363, 2425, 2484, 2544, 2602, 2662, 2716, 2776, 2841, 2901, 2960, 3016, 3071]}]}
Loading … Spinner
There are currently no alerts