FilmArray, an Automated Nested Multiplex PCR System for Multi-Pathogen Detection: Development and Application to Respiratory Tract Infection
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{"title"=>"Filmarray, an automated nested multiplex PCR system for multi-pathogen detection: Development and application to respiratory tract infection", "type"=>"journal", "authors"=>[{"first_name"=>"Mark A.", "last_name"=>"Poritz", "scopus_author_id"=>"6603354406"}, {"first_name"=>"Anne J.", "last_name"=>"Blaschke", "scopus_author_id"=>"6602179903"}, {"first_name"=>"Carrie L.", "last_name"=>"Byington", "scopus_author_id"=>"7003962116"}, {"first_name"=>"Lindsay", "last_name"=>"Meyers", "scopus_author_id"=>"53664058500"}, {"first_name"=>"Kody", "last_name"=>"Nilsson", "scopus_author_id"=>"54389804400"}, {"first_name"=>"David E.", "last_name"=>"Jones", "scopus_author_id"=>"57198726937"}, {"first_name"=>"Stephanie A.", "last_name"=>"Thatcher", "scopus_author_id"=>"54390245500"}, {"first_name"=>"Thomas", "last_name"=>"Robbins", "scopus_author_id"=>"24169635900"}, {"first_name"=>"Beth", "last_name"=>"Lingenfelter", "scopus_author_id"=>"6506895037"}, {"first_name"=>"Elizabeth", "last_name"=>"Amiott", "scopus_author_id"=>"13104603900"}, {"first_name"=>"Amy", "last_name"=>"Herbener", "scopus_author_id"=>"36097419700"}, {"first_name"=>"Judy", "last_name"=>"Daly", "scopus_author_id"=>"7401992507"}, {"first_name"=>"Steven F.", "last_name"=>"Dobrowolski", "scopus_author_id"=>"6603743865"}, {"first_name"=>"David H.F.", "last_name"=>"Teng", "scopus_author_id"=>"54390329800"}, {"first_name"=>"Kirk M.", "last_name"=>"Ririe", "scopus_author_id"=>"6505921604"}], "year"=>2011, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"22039434", "doi"=>"10.1371/journal.pone.0026047", "sgr"=>"80054778674", "isbn"=>"1932-6203", "scopus"=>"2-s2.0-80054778674", "issn"=>"19326203", "pui"=>"362778104"}, "id"=>"76ab54fb-58e9-3423-8675-1a2b5611ee2a", "abstract"=>"The ideal clinical diagnostic system should deliver rapid, sensitive, specific and reproducible results while minimizing the requirements for specialized laboratory facilities and skilled technicians. We describe an integrated diagnostic platform, the \"FilmArray\", which fully automates the detection and identification of multiple organisms from a single sample in about one hour. An unprocessed biologic/clinical sample is subjected to nucleic acid purification, reverse transcription, a high-order nested multiplex polymerase chain reaction and amplicon melt curve analysis. Biochemical reactions are enclosed in a disposable pouch, minimizing the PCR contamination risk. FilmArray has the potential to detect greater than 100 different nucleic acid targets at one time. These features make the system well-suited for molecular detection of infectious agents. Validation of the FilmArray technology was achieved through development of a panel of assays capable of identifying 21 common viral and bacterial respiratory pathogens. Initial testing of the system using both cultured organisms and clinical nasal aspirates obtained from children demonstrated an analytical and clinical sensitivity and specificity comparable to existing diagnostic platforms. We demonstrate that automated identification of pathogens from their corresponding target amplicon(s) can be accomplished by analysis of the DNA melting curve of the amplicon.", "link"=>"http://www.mendeley.com/research/filmarray-automated-nested-multiplex-pcr-system-multipathogen-detection-development-application-resp", "reader_count"=>117, "reader_count_by_academic_status"=>{"Unspecified"=>8, "Professor > Associate Professor"=>5, "Researcher"=>25, "Student > Doctoral Student"=>6, "Student > Ph. D. Student"=>28, "Student > Postgraduate"=>9, "Student > Master"=>9, "Other"=>12, "Student > Bachelor"=>9, "Professor"=>6}, "reader_count_by_user_role"=>{"Unspecified"=>8, "Professor > Associate Professor"=>5, "Researcher"=>25, "Student > Doctoral Student"=>6, "Student > Ph. D. Student"=>28, "Student > Postgraduate"=>9, "Student > Master"=>9, "Other"=>12, "Student > Bachelor"=>9, "Professor"=>6}, "reader_count_by_subject_area"=>{"Unspecified"=>8, "Agricultural and Biological Sciences"=>29, "Chemical Engineering"=>1, "Chemistry"=>3, "Computer Science"=>1, "Engineering"=>13, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>12, "Nursing and Health Professions"=>3, "Mathematics"=>1, "Medicine and Dentistry"=>33, "Sports and Recreations"=>1, "Social Sciences"=>3, "Immunology and Microbiology"=>7, "Linguistics"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>33}, "Social Sciences"=>{"Social Sciences"=>3}, "Sports and Recreations"=>{"Sports and Recreations"=>1}, "Mathematics"=>{"Mathematics"=>1}, "Unspecified"=>{"Unspecified"=>8}, "Environmental Science"=>{"Environmental Science"=>1}, "Chemical Engineering"=>{"Chemical Engineering"=>1}, "Engineering"=>{"Engineering"=>13}, "Chemistry"=>{"Chemistry"=>3}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>7}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>29}, "Computer Science"=>{"Computer Science"=>1}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>3}, "Linguistics"=>{"Linguistics"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>12}}, "reader_count_by_country"=>{"Colombia"=>1, "United States"=>4, "Denmark"=>1, "United Kingdom"=>2}, "group_count"=>3}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/722591"], "description"=>"<p>Pediatric NPA samples (N = 328) were tested either by DFA at PCMC (yellow bars) or on the FilmArray (Blue bars). The percent of samples in which no virus (Negative) or one of the indicated viruses was detected is shown. The viruses are grouped into those in which both DFA and FilmArray assays are available or only the FilmArray assay is available.</p>", "links"=>[], "tags"=>["rates", "filmarray", "rp", "pouch", "compared"], "article_id"=>392929, "categories"=>["Microbiology", "Biotechnology", "Virology", "Cell Biology", "Infectious Diseases"], "users"=>["Mark A. Poritz", "Anne J. Blaschke", "Carrie L. Byington", "Lindsay Meyers", "Kody Nilsson", "David E. Jones", "Stephanie A. Thatcher", "Thomas Robbins", "Beth Lingenfelter", "Elizabeth Amiott", "Amy Herbener", "Judy Daly", "Steven F. Dobrowolski", "David H. -F. Teng", "Kirk M. Ririe"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0026047.g005", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Detection_rates_of_the_FilmArray_RP_pouch_compared_to_DFA_/392929", "title"=>"Detection rates of the FilmArray RP pouch compared to DFA.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-10-19 00:48:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/722460"], "description"=>"<p>Respiratory Pathogen pouches were injected with viral transport medium spiked with 200 TCID<sub>50</sub> FluA H1-seasonal (panels <b>A</b> and <b>B</b>), 4×10<sup>6</sup> cfu B. per and 200 TCID<sub>50</sub> FluA-H1 (panels <b>C</b> and <b>D</b>), or 4×10<sup>6</sup> cfu B. per (panels <b>E</b> and <b>F</b>) and run on the FilmArray instrument. Real time amplification curves (panels <b>A</b> and <b>C</b> and <b>E</b>) and post-amplification melt curves (panels <b>B</b> and <b>D</b> and <b>F</b>) for selected wells on the array are shown. Assays are spotted in triplicate: FluA-pan1 (orange), FluA-pan2 (pink), FluA-H1-pan (red), FluA-H3 (black), B. per (Green), Yeast RNA process control (dark blue), Second stage PCR control (light blue). For clarity the controls are shown in panels <b>E</b> and <b>F</b> only.</p>", "links"=>[], "tags"=>["amplification", "curves"], "article_id"=>392803, "categories"=>["Microbiology", "Biotechnology", "Virology", "Cell Biology", "Infectious Diseases"], "users"=>["Mark A. Poritz", "Anne J. Blaschke", "Carrie L. Byington", "Lindsay Meyers", "Kody Nilsson", "David E. Jones", "Stephanie A. Thatcher", "Thomas Robbins", "Beth Lingenfelter", "Elizabeth Amiott", "Amy Herbener", "Judy Daly", "Steven F. Dobrowolski", "David H. -F. Teng", "Kirk M. Ririe"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0026047.g004", "stats"=>{"downloads"=>1, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Real_time_amplification_and_melt_curves_from_the_array_/392803", "title"=>"Real-time amplification and melt curves from the array.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-10-19 00:46:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/722293"], "description"=>"<p>The layers of film and adhesive attaching the array to the pouch are separated to show the flow of liquid into the cells of the array (figure is not to scale). From the top the layers are: 2<sup>nd</sup> pouch film, 1st pouch film, array adhesive layer (orange), pricked cover film, array (black, with wells), and array cover film. All of the actual layers are transparent except for the array itself. Second stage PCR primers are spotted into the cells during manufacture and air-dried (Methods). Arrows show the flow of PCR master mix (without primers) entering the array through a hole cut in the 1<sup>st</sup> pouch film.</p>", "links"=>[], "tags"=>["pcr", "entering"], "article_id"=>392641, "categories"=>["Microbiology", "Biotechnology", "Virology", "Cell Biology", "Infectious Diseases"], "users"=>["Mark A. Poritz", "Anne J. Blaschke", "Carrie L. Byington", "Lindsay Meyers", "Kody Nilsson", "David E. Jones", "Stephanie A. Thatcher", "Thomas Robbins", "Beth Lingenfelter", "Elizabeth Amiott", "Amy Herbener", "Judy Daly", "Steven F. Dobrowolski", "David H. -F. Teng", "Kirk M. Ririe"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0026047.g002", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Schematic_of_second_stage_PCR_mix_entering_the_array_/392641", "title"=>"Schematic of second stage PCR mix entering the array.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-10-19 00:44:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/722965"], "description"=>"a<p>Positive or Negative test result comparing FilmArray RP (new test) to DFA (reference standard subject to error). (N = 328)</p>b<p>Clopper-Pearson 95% confidence Interval.</p>c<p>McNemar test, comparing discordant cells (FilmArray positive, DFA negative) vs (FilmArray negative, DFA positive).</p>", "links"=>[], "tags"=>["filmarray", "rp"], "article_id"=>393308, "categories"=>["Microbiology", "Biotechnology", "Virology", "Cell Biology", "Infectious Diseases"], "users"=>["Mark A. Poritz", "Anne J. Blaschke", "Carrie L. Byington", "Lindsay Meyers", "Kody Nilsson", "David E. Jones", "Stephanie A. Thatcher", "Thomas Robbins", "Beth Lingenfelter", "Elizabeth Amiott", "Amy Herbener", "Judy Daly", "Steven F. Dobrowolski", "David H. -F. Teng", "Kirk M. Ririe"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0026047.t002", "stats"=>{"downloads"=>7, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparison_of_FilmArray_RP_to_DFA_/393308", "title"=>"Comparison of FilmArray RP to DFA.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2011-10-19 00:55:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/722915"], "description"=>"<p>Performance of the FilmArray RP melt curve detection algorithm compared to expert interpretation.</p>", "links"=>[], "tags"=>["filmarray", "rp", "detection", "algorithm", "compared"], "article_id"=>393258, "categories"=>["Microbiology", "Biotechnology", "Virology", "Cell Biology", "Infectious Diseases"], "users"=>["Mark A. Poritz", "Anne J. Blaschke", "Carrie L. Byington", "Lindsay Meyers", "Kody Nilsson", "David E. Jones", "Stephanie A. Thatcher", "Thomas Robbins", "Beth Lingenfelter", "Elizabeth Amiott", "Amy Herbener", "Judy Daly", "Steven F. Dobrowolski", "David H. -F. Teng", "Kirk M. Ririe"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0026047.t003", "stats"=>{"downloads"=>4, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Performance_of_the_FilmArray_RP_melt_curve_detection_algorithm_compared_to_expert_interpretation_/393258", "title"=>"Performance of the FilmArray RP melt curve detection algorithm compared to expert interpretation.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2011-10-19 00:54:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/722782"], "description"=>"<p>Histograms of the theoretical or observed T<sub>m</sub>s of the hMPV assay are shown. T<sub>m</sub> data for the FilmArray runs includes each of the three replicates of the second stage PCR. <b>A:</b> T<sub>m</sub>s calculated from 13 sequence variants published in the NCBI databases. <b>B:</b> T<sub>m</sub> data generated during the system beta-testing with 37 banked hMPV-positive patient samples. <b>C:</b> T<sub>m</sub> data generated during the inclusivity testing with 10 hMPV strains representing subtypes A1, A2, B1 and B2. Multiple FilmArray runs of these strains are included in this data set. <b>D:</b> T<sub>m</sub> data from 74 hMPV-positive patient samples collected during the clinical evaluation.</p>", "links"=>[], "tags"=>["assay"], "article_id"=>393131, "categories"=>["Microbiology", "Biotechnology", "Virology", "Cell Biology", "Infectious Diseases"], "users"=>["Mark A. Poritz", "Anne J. Blaschke", "Carrie L. Byington", "Lindsay Meyers", "Kody Nilsson", "David E. Jones", "Stephanie A. Thatcher", "Thomas Robbins", "Beth Lingenfelter", "Elizabeth Amiott", "Amy Herbener", "Judy Daly", "Steven F. Dobrowolski", "David H. -F. Teng", "Kirk M. Ririe"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0026047.g007", "stats"=>{"downloads"=>1, "page_views"=>70, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_T_m_data_used_to_establish_assay_specific_melt_windows_/393131", "title"=>"T<sub>m</sub> data used to establish assay specific melt windows.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-10-19 00:52:11"}
  • {"files"=>["https://ndownloader.figshare.com/files/722156"], "description"=>"<p>(<b><i>A</i></b>) A FilmArray pouch was injected with mock sample (here colored blue for illustrative purposes) in the left side injection port and hydration solution (colored red) in the right side injection port. (<b>B</b>) The blisters of a FilmArray pouch were filled with different coloring (and the channels between the blisters heat sealed shut). In this pouch the plunger tree was made from plastic dyed blue. The fitment and film are normally at right angles to each other; for clarity the pouch has been flattened. (<b>C</b>) A schematic of the pouch showing a trace of the blisters, channels and array wells (black) and the functional areas of the pouch (red).</p>", "links"=>[], "tags"=>["Virology", "Infectious diseases", "microbiology", "biotechnology", "pathology"], "article_id"=>392497, "categories"=>["Microbiology", "Biotechnology", "Virology", "Cell Biology", "Infectious Diseases"], "users"=>["Mark A. Poritz", "Anne J. Blaschke", "Carrie L. Byington", "Lindsay Meyers", "Kody Nilsson", "David E. Jones", "Stephanie A. Thatcher", "Thomas Robbins", "Beth Lingenfelter", "Elizabeth Amiott", "Amy Herbener", "Judy Daly", "Steven F. Dobrowolski", "David H. -F. Teng", "Kirk M. Ririe"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0026047.g001", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_FilmArray_pouch_/392497", "title"=>"FilmArray pouch.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-10-19 00:41:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/723018"], "description"=>"<p>Performance of the FilmArray RP system automated analysis as compared to expert interpretation.</p>", "links"=>[], "tags"=>["filmarray", "rp", "automated", "compared"], "article_id"=>393362, "categories"=>["Microbiology", "Biotechnology", "Virology", "Cell Biology", "Infectious Diseases"], "users"=>["Mark A. Poritz", "Anne J. Blaschke", "Carrie L. Byington", "Lindsay Meyers", "Kody Nilsson", "David E. Jones", "Stephanie A. Thatcher", "Thomas Robbins", "Beth Lingenfelter", "Elizabeth Amiott", "Amy Herbener", "Judy Daly", "Steven F. Dobrowolski", "David H. -F. Teng", "Kirk M. Ririe"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0026047.t004", "stats"=>{"downloads"=>4, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Performance_of_the_FilmArray_RP_system_automated_analysis_as_compared_to_expert_interpretation_/393362", "title"=>"Performance of the FilmArray RP system automated analysis as compared to expert interpretation.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2011-10-19 00:56:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/365370"], "description"=>"<div><p>The ideal clinical diagnostic system should deliver rapid, sensitive, specific and reproducible results while minimizing the requirements for specialized laboratory facilities and skilled technicians. We describe an integrated diagnostic platform, the “FilmArray”, which fully automates the detection and identification of multiple organisms from a single sample in about one hour. An unprocessed biologic/clinical sample is subjected to nucleic acid purification, reverse transcription, a high-order nested multiplex polymerase chain reaction and amplicon melt curve analysis. Biochemical reactions are enclosed in a disposable pouch, minimizing the PCR contamination risk. FilmArray has the potential to detect greater than 100 different nucleic acid targets at one time. These features make the system well-suited for molecular detection of infectious agents. Validation of the FilmArray technology was achieved through development of a panel of assays capable of identifying 21 common viral and bacterial respiratory pathogens. Initial testing of the system using both cultured organisms and clinical nasal aspirates obtained from children demonstrated an analytical and clinical sensitivity and specificity comparable to existing diagnostic platforms. We demonstrate that automated identification of pathogens from their corresponding target amplicon(s) can be accomplished by analysis of the DNA melting curve of the amplicon.</p> </div>", "links"=>[], "tags"=>["automated", "nested", "multiplex", "pcr", "multi-pathogen", "respiratory", "tract"], "article_id"=>132212, "categories"=>["Biotechnology", "Cancer", "Cell Biology", "Microbiology"], "users"=>["Mark A. Poritz", "Anne J. Blaschke", "Carrie L. Byington", "Lindsay Meyers", "Kody Nilsson", "David E. Jones", "Stephanie A. Thatcher", "Thomas Robbins", "Beth Lingenfelter", "Elizabeth Amiott", "Amy Herbener", "Judy Daly", "Steven F. Dobrowolski", "David H. -F. Teng", "Kirk M. Ririe"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0026047", "stats"=>{"downloads"=>3, "page_views"=>29, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/FilmArray_an_Automated_Nested_Multiplex_PCR_System_for_Multi_Pathogen_Detection_Development_and_Application_to_Respiratory_Tract_Infection/132212", "title"=>"FilmArray, an Automated Nested Multiplex PCR System for Multi-Pathogen Detection: Development and Application to Respiratory Tract Infection", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-10-19 00:36:52"}
  • {"files"=>["https://ndownloader.figshare.com/files/722381"], "description"=>"<p>FilmArray instrument with pouch being loaded.</p>", "links"=>[], "tags"=>["pouch"], "article_id"=>392728, "categories"=>["Microbiology", "Biotechnology", "Virology", "Cell Biology", "Infectious Diseases"], "users"=>["Mark A. Poritz", "Anne J. Blaschke", "Carrie L. Byington", "Lindsay Meyers", "Kody Nilsson", "David E. Jones", "Stephanie A. Thatcher", "Thomas Robbins", "Beth Lingenfelter", "Elizabeth Amiott", "Amy Herbener", "Judy Daly", "Steven F. Dobrowolski", "David H. -F. Teng", "Kirk M. Ririe"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0026047.g003", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_FilmArray_instrument_with_pouch_being_loaded_/392728", "title"=>"FilmArray instrument with pouch being loaded.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-10-19 00:45:28"}
  • {"files"=>["https://ndownloader.figshare.com/files/722704"], "description"=>"<p>Respiratory Pathogen pouches were injected with viral transport medium spiked with 1 TCID<sub>50</sub> of the FluA- H1 seasonal virus used in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0026047#pone-0026047-g004\" target=\"_blank\">Figure 4</a>, and run on the FilmArray instrument. Real-time amplification curves (<b>A</b>) and post-amplification melt curves (<b>B</b>) for selected wells on the array are shown. Assays are spotted in triplicate: FluA-pan1 (orange), FluA-pan2 (pink), FluA-H1-pan (red). The ordinate scales are the same as in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0026047#pone-0026047-g004\" target=\"_blank\">Figure 4</a>.</p>", "links"=>[], "tags"=>["curves"], "article_id"=>393052, "categories"=>["Microbiology", "Biotechnology", "Virology", "Cell Biology", "Infectious Diseases"], "users"=>["Mark A. Poritz", "Anne J. Blaschke", "Carrie L. Byington", "Lindsay Meyers", "Kody Nilsson", "David E. Jones", "Stephanie A. Thatcher", "Thomas Robbins", "Beth Lingenfelter", "Elizabeth Amiott", "Amy Herbener", "Judy Daly", "Steven F. Dobrowolski", "David H. -F. Teng", "Kirk M. Ririe"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0026047.g006", "stats"=>{"downloads"=>2, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Amplification_and_melt_curves_at_low_target_levels_/393052", "title"=>"Amplification and melt curves at low target levels.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-10-19 00:50:52"}
  • {"files"=>["https://ndownloader.figshare.com/files/722870"], "description"=>"a<p>See <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0026047#pone.0026047.s001\" target=\"_blank\">Table S1</a> for the source of the organisms.</p>b<p>LoD concentrations are expressed in CFU/ml and TCID<sub>50</sub>/mL for bacteria and viruses respectively except for <i>C. pne</i> and BoV (DNA copies/mL) and CoV-HKU1 (RNA copies/ml) respectively (Methods).</p>c<p>The LoD for Enterovirus (30,000 TCID<sub>50</sub>/ml) is based on positive results for the Entero1 or Entero2 assays. A final result of Human Rhinovirus/Enterovirus based on the combination of 6 different assays (HRV1–4, Entero1 and Entero2) can be obtained at much lower concentrations (∼300 TCID<sub>50</sub>/mL).</p>d<p>The Flu A Matrix and NS1 gene assays are referred to as “pan1” and “pan2” respectively in the text.</p><p>AV, Adenovirus; B. per, Bordetella pertussis; BoV, Bocavirus; <i>C. pne</i>, <i>Chlamydophila pneumoniae</i>; CoV, Coronavirus; EV, Enterovirus; FluA, Influenza A ; FluB, Influenza B; hMPV, Human metapneumovirus ; HRV, human Rhinovirus; <i>M. pne</i>, <i>Mycoplasma pneumoniae</i>; PIV1–4, Parainfluenza viruses 1–4; RSV, Respiratory Syncytial Virus.</p>", "links"=>[], "tags"=>["rp", "pouch", "targets"], "article_id"=>393213, "categories"=>["Microbiology", "Biotechnology", "Virology", "Cell Biology", "Infectious Diseases"], "users"=>["Mark A. Poritz", "Anne J. Blaschke", "Carrie L. Byington", "Lindsay Meyers", "Kody Nilsson", "David E. Jones", "Stephanie A. Thatcher", "Thomas Robbins", "Beth Lingenfelter", "Elizabeth Amiott", "Amy Herbener", "Judy Daly", "Steven F. Dobrowolski", "David H. -F. Teng", "Kirk M. Ririe"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0026047.t001", "stats"=>{"downloads"=>4, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_FilmArray_RP_Pouch_Pathogens_Gene_Targets_and_LOD_95_/393213", "title"=>"FilmArray RP Pouch Pathogens, Gene Targets and LOD<sub>95</sub>.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2011-10-19 00:53:33"}

PMC Usage Stats | Further Information

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Relative Metric

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