Microtubule Dynamics Regulate Cyclic Stretch-Induced Cell Alignment in Human Airway Smooth Muscle Cells
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{"title"=>"Microtubule dynamics regulate cyclic stretch-induced cell alignment in human airway smooth muscle cells", "type"=>"journal", "authors"=>[{"first_name"=>"Masataka", "last_name"=>"Morioka", "scopus_author_id"=>"34971694400"}, {"first_name"=>"Harikrishnan", "last_name"=>"Parameswaran", "scopus_author_id"=>"6507061695"}, {"first_name"=>"Keiji", "last_name"=>"Naruse", "scopus_author_id"=>"7102661918"}, {"first_name"=>"Masashi", "last_name"=>"Kondo", "scopus_author_id"=>"7403404998"}, {"first_name"=>"Masahiro", "last_name"=>"Sokabe", "scopus_author_id"=>"7006682509"}, {"first_name"=>"Yoshinori", "last_name"=>"Hasegawa", "scopus_author_id"=>"7403042668"}, {"first_name"=>"Béla", "last_name"=>"Suki", "scopus_author_id"=>"7005193754"}, {"first_name"=>"Satoru", "last_name"=>"Ito", "scopus_author_id"=>"55095580800"}], "year"=>2011, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-80054752373", "sgr"=>"80054752373", "pui"=>"362771692", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pmid"=>"22022610", "doi"=>"10.1371/journal.pone.0026384"}, "id"=>"9161fed3-5260-32f6-8ca7-513754254c70", "abstract"=>"Microtubules are structural components of the cytoskeleton that determine cell shape, polarity, and motility in cooperation with the actin filaments. In order to determine the role of microtubules in cell alignment, human airway smooth muscle cells were exposed to cyclic uniaxial stretch. Human airway smooth muscle cells, cultured on type I collagen-coated elastic silicone membranes, were stretched uniaxially (20% in strain, 30 cycles/min) for 2 h. The population of airway smooth muscle cells which were originally oriented randomly aligned near perpendicular to the stretch axis in a time-dependent manner. However, when the cells treated with microtubule disruptors, nocodazole and colchicine, were subjected to the same cyclic uniaxial stretch, the cells failed to align. Lack of alignment was also observed for airway smooth muscle cells treated with a microtubule stabilizer, paclitaxel. To understand the intracellular mechanisms involved, we developed a computational model in which microtubule polymerization and attachment to focal adhesions were regulated by the preexisting tensile stress, pre-stress, on actin stress fibers. We demonstrate that microtubules play a central role in cell re-orientation when cells experience cyclic uniaxial stretching. Our findings further suggest that cell alignment and cytoskeletal reorganization in response to cyclic stretch results from the ability of the microtubule-stress fiber assembly to maintain a homeostatic strain on the stress fiber at focal adhesions. The mechanism of stretch-induced alignment we uncovered is likely involved in various airway functions as well as in the pathophysiology of airway remodeling in asthma.", "link"=>"http://www.mendeley.com/research/microtubule-dynamics-regulate-cyclic-stretchinduced-cell-alignment-human-airway-smooth-muscle-cells", "reader_count"=>48, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>2, "Researcher"=>8, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>22, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>3, "Student > Bachelor"=>1, "Professor"=>5}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>2, "Researcher"=>8, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>22, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>3, "Student > Bachelor"=>1, "Professor"=>5}, "reader_count_by_subject_area"=>{"Engineering"=>11, "Unspecified"=>4, "Biochemistry, Genetics and Molecular Biology"=>4, "Materials Science"=>4, "Mathematics"=>2, "Agricultural and Biological Sciences"=>15, "Medicine and Dentistry"=>2, "Neuroscience"=>1, "Physics and Astronomy"=>4, "Psychology"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>11}, "Materials Science"=>{"Materials Science"=>4}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Neuroscience"=>{"Neuroscience"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>4}, "Psychology"=>{"Psychology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>15}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}, "Mathematics"=>{"Mathematics"=>2}, "Unspecified"=>{"Unspecified"=>4}}, "reader_count_by_country"=>{"United States"=>1}, "group_count"=>1}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/723713"], "description"=>"<p>The angle is always between 0° and 90° with respect to the stretch axis (arrows).</p>", "links"=>[], "tags"=>["axis", "was"], "article_id"=>394061, "categories"=>["Biophysics"], "users"=>["Masataka Morioka", "Harikrishnan Parameswaran", "Keiji Naruse", "Masashi Kondo", "Masahiro Sokabe", "Yoshinori Hasegawa", "Béla Suki", "Satoru Ito"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0026384.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Schematic_of_how_the_angle_of_orientation_952_of_the_long_axis_was_measured_/394061", "title"=>"Schematic of how the angle of orientation (θ) of the long axis was measured.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 13:43:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/723743"], "description"=>"<p>(<b>A</b>) Model prediction of changes in shape and orientation of cells due to cyclic uniaxial stretching. A cell which was initially oriented parallel with the direction of stretch, first becomes circular before realigning in the direction of minimum strain. The extent to which the cell changes its shape is a function of its initial orientation; with cells that were initially aligned orthogonal to the direction of stretch experiencing very little shape changes due to stretch. (<b>B</b>) Histogram of a population of cells with the same initial orientation as the experiment at time t = 0, realigning in response to stretch. It can be seen that the first cells to realign are those that are oriented parallel to the direction of stretch. The inset shows the experimental observed histogram of cell orientations at time t = 2 h with results from the model overlaid on top.</p>", "links"=>[], "tags"=>["microtubules"], "article_id"=>394092, "categories"=>["Biophysics"], "users"=>["Masataka Morioka", "Harikrishnan Parameswaran", "Keiji Naruse", "Masashi Kondo", "Masahiro Sokabe", "Yoshinori Hasegawa", "Béla Suki", "Satoru Ito"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0026384.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Modeling_the_Role_of_Microtubules_in_Cell_Reorientation_/394092", "title"=>"Modeling the Role of Microtubules in Cell Reorientation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 13:43:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/723830"], "description"=>"<p>Phase-contrast images of the cells in the static condition (<b>A</b>) or in response to cyclic stretch (20% in strain, 30 cycle/min) (<b>B</b>). An arrow indicates stretch direction. Histograms of the orientation response of the cells in the static condition (<b>C</b>) or to 2 h uniaxial cyclic stretch (<b>D</b>). Images of the unstretched and stretched cells were obtained at 0.5 h, 1 h, and 2 h after the onset of cell stretching. After the cell angles were measured, the angles were binned into 19 groups for every 5°: 0°, 1–5°, 6–10°, …, 81–85°, and 86–90° (<b>C</b> and <b>D</b>). Total cell number (frequency) was set as 100% and the frequency in each group was expressed relative to the total cell number. (<b>E</b> and <b>F</b>) The mean and standard deviation (SD) of the cell orientation at each time point. The decreasing SD of angles indicates that the cells were aligning in a direction which was almost perpendicular to the direction of applied stretch. Bar graph represents means ± SD (across 5 different trials). Data were analyzed with one-way repeated-measure ANOVA followed by the Bonferroni test. *: Significantly different (P<0.05) from the unstretched control (time 0) value (n = 5).</p>", "links"=>[], "tags"=>["cyclic"], "article_id"=>394187, "categories"=>["Biophysics"], "users"=>["Masataka Morioka", "Harikrishnan Parameswaran", "Keiji Naruse", "Masashi Kondo", "Masahiro Sokabe", "Yoshinori Hasegawa", "Béla Suki", "Satoru Ito"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0026384.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_cyclic_stretch_on_cell_reorientation_/394187", "title"=>"Effects of cyclic stretch on cell reorientation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 13:44:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/723971"], "description"=>"<p>Cells were in the static condition (<i>upper panels</i>) or subjected to 2 h uniaxial cyclic stretch (<i>lower panels</i>). F-actin was visualized with rhodamine-phalloidin (red). Microtubules were visualized with FITC conjugated secondary antibody following immunostaining with anti-α-tubulin antibody (green). Cell nuclei were stained with DAPI (cyan).</p>", "links"=>[], "tags"=>["images", "f-actin", "microtubules", "static", "stretched"], "article_id"=>394318, "categories"=>["Biophysics"], "users"=>["Masataka Morioka", "Harikrishnan Parameswaran", "Keiji Naruse", "Masashi Kondo", "Masahiro Sokabe", "Yoshinori Hasegawa", "Béla Suki", "Satoru Ito"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0026384.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Fluorescent_images_of_organization_of_F_actin_and_microtubules_in_the_static_or_stretched_cells_/394318", "title"=>"Fluorescent images of organization of F-actin and microtubules in the static or stretched cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 13:45:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/724118"], "description"=>"<p>Cell spreading was quantified by measuring the area of cells from phase contrast images of cells at time 0, 0.5 h, 1 h, and 2 h after the start of stretch. Bar graphs represent means ± SD (n = 4). *: Significantly different (P<0.05) from the value with unstretched controls (time 0).</p>", "links"=>[], "tags"=>["spreading"], "article_id"=>394470, "categories"=>["Biophysics"], "users"=>["Masataka Morioka", "Harikrishnan Parameswaran", "Keiji Naruse", "Masashi Kondo", "Masahiro Sokabe", "Yoshinori Hasegawa", "Béla Suki", "Satoru Ito"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0026384.g005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Stretch_induced_spreading_of_cells_/394470", "title"=>"Stretch-induced spreading of cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 13:45:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/724244"], "description"=>"<p>Cyclic stretch (20%, 2 h) was applied to the cells after pretreatment with either 1 µM nocodazole (NDZ), 10 µM colchicine (COL), or 1 µM paclitaxel (PTX). (<b>A</b>) Representative phase-contrast images of the static (<i>left panels</i>) and stretched (<i>right panels</i>) cells pretreated with either 1 µM nocodazole (NDZ) (<i>upper panels</i>) or 1 µM paclitaxel (PTX) (<i>lower panels</i>). Average (<b>B</b>) and SDs (<b>C</b>) of angles of the cell orientation. Bar graphs represent means ± SD (n = 4). *: Significantly different (P<0.05) from the value with cyclic stretch. #: Significantly different (P<0.05) from the value with unstretched control.</p>", "links"=>[], "tags"=>["microtubules", "stretch-induced"], "article_id"=>394595, "categories"=>["Biophysics"], "users"=>["Masataka Morioka", "Harikrishnan Parameswaran", "Keiji Naruse", "Masashi Kondo", "Masahiro Sokabe", "Yoshinori Hasegawa", "Béla Suki", "Satoru Ito"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0026384.g006"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Roles_of_microtubules_in_stretch_induced_cell_reorientation_/394595", "title"=>"Roles of microtubules in stretch-induced cell reorientation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 13:46:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/724388"], "description"=>"<p>Fluorescent images of the organization of F-actin stained with rhodamine-phalloidin (red) and microtubules immunostained with anti-α-tubulin antibody (green) in the static or stretched cells. Cell nuclei were stained with DAPI (cyan). The cells were pretreated with either 1 µM nocodazole (NDZ; <b>A</b>) or 1 µM paclitaxel (PTX; <b>B</b>). Arrows indicate stretch direction.</p>", "links"=>[], "tags"=>["nocodazole", "paclitaxel", "f-actin"], "article_id"=>394741, "categories"=>["Biophysics"], "users"=>["Masataka Morioka", "Harikrishnan Parameswaran", "Keiji Naruse", "Masashi Kondo", "Masahiro Sokabe", "Yoshinori Hasegawa", "Béla Suki", "Satoru Ito"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0026384.g007"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_nocodazole_and_paclitaxel_on_the_organization_of_F_actin_and_microtubules_/394741", "title"=>"Effects of nocodazole and paclitaxel on the organization of F-actin and microtubules.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 13:47:12"}

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Relative Metric

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