A Retinoic Acid Responsive Hoxa3 Transgene Expressed in Embryonic Pharyngeal Endoderm, Cardiac Neural Crest and a Subdomain of the Second Heart Field
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{"title"=>"A retinoic acid responsive Hoxa3 transgene expressed in embryonic pharyngeal endoderm, cardiac neural crest and a subdomain of the second heart field", "type"=>"journal", "authors"=>[{"first_name"=>"Nata Y.S.G.", "last_name"=>"Diman", "scopus_author_id"=>"6507864825"}, {"first_name"=>"Sophie", "last_name"=>"Remacle", "scopus_author_id"=>"8085849900"}, {"first_name"=>"Nicolas", "last_name"=>"Bertrand", "scopus_author_id"=>"57198098791"}, {"first_name"=>"Jacques J.", "last_name"=>"Picard", "scopus_author_id"=>"7201606733"}, {"first_name"=>"Stéphane", "last_name"=>"Zaffran", "scopus_author_id"=>"6603478871"}, {"first_name"=>"René", "last_name"=>"Rezsohazy", "scopus_author_id"=>"6603441196"}], "year"=>2011, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-81255172962", "sgr"=>"81255172962", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0027624", "pmid"=>"22110697", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pui"=>"362926437"}, "id"=>"b3b15ca3-bf59-3473-a478-31f517be0a9f", "abstract"=>"A transgenic mouse line harbouring a β-galacdosidase reporter gene controlled by the proximal 2 kb promoter of Hoxa3 was previously generated to investigate the regulatory cues governing Hoxa3 expression in the mouse. Examination of transgenic embryos from embryonic day (E) 8.0 to E15.5 revealed regionally restricted reporter activity in the developing heart. Indeed, transgene expression specifically delineated cells from three distinct lineages: a subpopulation of the second heart field contributing to outflow tract myocardium, the cardiac neural crest cells and the pharyngeal endoderm. Manipulation of the Retinoic Acid (RA) signaling pathway showed that RA is required for correct expression of the transgene. Therefore, this transgenic line may serve as a cardiosensor line of particular interest for further analysis of outflow tract development.", "link"=>"http://www.mendeley.com/research/retinoic-acid-responsive-hoxa3-transgene-expressed-embryonic-pharyngeal-endoderm-cardiac-neural-cres", "reader_count"=>29, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Librarian"=>1, "Researcher"=>4, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>10, "Student > Postgraduate"=>1, "Student > Master"=>4, "Other"=>1, "Student > Bachelor"=>3, "Professor"=>3}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Librarian"=>1, "Researcher"=>4, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>10, "Student > Postgraduate"=>1, "Student > Master"=>4, "Other"=>1, "Student > Bachelor"=>3, "Professor"=>3}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>3, "Agricultural and Biological Sciences"=>25, "Medicine and Dentistry"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>25}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}}, "reader_count_by_country"=>{"United States"=>1, "Brazil"=>1}, "group_count"=>1}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/711469"], "description"=>"<p>(A, B, C) Lateral view of E8.5 (A), E9.5 (B) and E10.5 (C) transgenic embryos stained for <i>lacZ</i> activity and corresponding magnification of the pharyngeal and heart region, (D, E and F, respectively). Arrowheads in A and B indicate the lateral plate mesoderm; the arrowhead in D shows the pharyngeal endoderm; the arrow in F indicates the pharyngeal mesoderm lying caudally to the OFT. Asterisks in A-to-C indicate the otic vesicle. Numbering 1, 2, 3, 4 and 6 indicates the pharyngeal arches. FL; forelimb; Ht, heart; HL, hindlimb. Scale bar = 100 µm.</p>", "links"=>[], "tags"=>["transgene", "embryonic"], "article_id"=>381828, "categories"=>["Molecular Biology", "Biotechnology", "Developmental Biology", "Biological Sciences"], "users"=>["Nata Y. S.-G. Diman", "Sophie Remacle", "Nicolas Bertrand", "Jacques J. Picard", "Stéphane Zaffran", "René Rezsohazy"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0027624.g001", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Time_course_of_Hoxa3_lacZ_transgene_activity_at_early_embryonic_stages_/381828", "title"=>"Time course of <i>Hoxa3-lacZ</i> transgene activity at early embryonic stages.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-11-16 00:30:28"}
  • {"files"=>["https://ndownloader.figshare.com/files/711697"], "description"=>"<p>Ventral views of whole mount hearts X-gal stained at E9.5 (A), E10.5 (B), E13.5 (C) and E15.5 (D). (A) X-gal stained cells are present in the inferior and superior walls of the distal OFT (arrowheads), and in the aortic sac indicated by the asterick. (B) ß-gal activity is extended in the proximal OFT and clearly visible in the aortic sac but still excluded from atria and ventricles (arrowheads indicate the transverse section level corresponding to the image inset). (C) Labeled cells are detected in the aortic and pulmonary trunk at E12.5. (D). At E15.5 X-gal staining in the aorta and pulmonary trunk includes the myocardium at the base of these vessels. At all these stages <i>Hoxa3-lacZ</i> is silent in the atria and ventricles (A–D). Ao, aorta; IA, innominate artery; LA, left atrium; LCA, left common artery; LSA left subclavian artery, LV, left ventricle; OFT, outflow tract; PT, pulmonary trunk; RA, right atrium; RCA, right common artery; RSA, rigth subclavian artery;RV, right ventricle. Scale bars = 100 µm.</p>", "links"=>[], "tags"=>["transgene"], "article_id"=>382055, "categories"=>["Molecular Biology", "Biotechnology", "Developmental Biology", "Biological Sciences"], "users"=>["Nata Y. S.-G. Diman", "Sophie Remacle", "Nicolas Bertrand", "Jacques J. Picard", "Stéphane Zaffran", "René Rezsohazy"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0027624.g002", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_of_the_Hoxa3_lacZ_transgene_during_cardiogenesis_/382055", "title"=>"Expression of the <i>Hoxa3-lacZ</i> transgene during cardiogenesis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-11-16 00:34:15"}
  • {"files"=>["https://ndownloader.figshare.com/files/712153"], "description"=>"<p>Transverse sections of E11.5 (A-C), and E13.5 (D-G) developing hearts (A) X-gal-labeled cells are present in the cushion of E11.5 embryos (asterisks) and (B) on adjacent section, α-SMA staining shows that <i>lacZ</i>-expressing cells correspond to cardiac neural crest differentiating into smooth muscle that (C) are negative for c-actin expression and are thus not muscularized. Arrowheads in C indicate myocardial spikes protruding toward the cushion mesenchyme. (D) X-gal stained cells are detected in the aortic and pulmonary trunk as well as in the ductus arteriosus (arrowhead). (E) These <i>lacZ</i>-positive cells also express α-SMA. (F) At E13.5, the outlet septum stains positive for X-gal (arrowhead). (G) c-actin immunostaining demonstrates that myocardialization occurs in this region as well as in the cushion at base of the aorta. Ao, aorta; DAo, dorsal aorta; LA, left atrium; OFT, outflow tract; Ot, outlet; PT, pulmonary trunk; RA, right atrium; RV, right ventricle; Tr, Trachea. Scale bars = 100 µm.</p>", "links"=>[], "tags"=>["x-gal-labeled", "cells", "transgenic", "hearts", "outflow", "tract"], "article_id"=>382506, "categories"=>["Molecular Biology", "Biotechnology", "Developmental Biology", "Biological Sciences"], "users"=>["Nata Y. S.-G. Diman", "Sophie Remacle", "Nicolas Bertrand", "Jacques J. Picard", "Stéphane Zaffran", "René Rezsohazy"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0027624.g004", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Distribution_of_X_gal_labeled_cells_in_transgenic_hearts_before_E11_5_and_after_E13_5_outflow_tract_septation_/382506", "title"=>"Distribution of X-gal-labeled cells in transgenic hearts before (E11.5) and after (E13.5) outflow tract septation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-11-16 00:41:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/712897"], "description"=>"<p>At E8.5 <i>Mlc1v-nlacZ-24</i> are activated in the second heart field, developing OFT and heart tube (A), while <i>Hoxa3-lacZ</i> is activated in PE and barely detected in the SHF (B). At this stage the heart is devoid of migrating cardiac neural crest cells (C). At E9.5 the SHF progenitors, OFT and RV myocardium are labeled in <i>Mlc1v-nlacZ-24</i> (D). The PE, CNC (dots in the caudal pharynx) and a subdomain of the SHF express <i>Hoxa3-lacZ</i> (E). Cardiac neural crest cells are also labeled in <i>Wnt1cre;R26R</i> bigenic embryos at E9.5 (F). At E10.5, <i>Mlc1v-nlacZ-24</i> transgenic hearts identify SHF, OFT and RV (G). In addition to the anterior portion of SHF and PE, <i>Hoxa3-lacZ</i> transgene activity identifies cardiac neural crest and myocardial cells of the OFT, whereas the RV remains negative for <i>lacZ</i> staining (E). Cardiac neural crest cells similarly invades the OFT in <i>Wnt1cre;R26R</i> bigenic embryos at E10.5 (I). <i>Hoxa3-lacZ</i> clearly appears as an intermediary reporter line that display hybrid expression pattern reminiscent to that of Mlc1v-nlacZ24 and <i>Wnt1cre;R26R</i> bigenic. Blue and pink colors indicate <i>lacZ</i>-positive and -negative cells, respectively. Black and red arrowheads designate the pharyngeal endoderm (PE) and second heart field (SHF), respectively. A, atria; HT, heart tube; Oft, outflow tract; RA, right atria; RV, rigth ventricle.</p>", "links"=>[], "tags"=>["cardiosensor"], "article_id"=>383257, "categories"=>["Molecular Biology", "Biotechnology", "Developmental Biology", "Biological Sciences"], "users"=>["Nata Y. S.-G. Diman", "Sophie Remacle", "Nicolas Bertrand", "Jacques J. Picard", "Stéphane Zaffran", "René Rezsohazy"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0027624.g007", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Schematic_view_of_lacZ_expression_pattern_in_of_Mlc1v_nlacZ24_Hoxa3_lacZ_and_Wnt1Cre_R26R_cardiosensor_lines_Mlc1v_nlacZ24_A_D_G_Hoxa3_lacZ_B_E_H_and_Wnt1_Cre_R26R_C_F_I_/383257", "title"=>"Schematic view of <i>lacZ</i> expression pattern in of <i>Mlc1v-nlacZ24, Hoxa3-lacZ</i> and <i>Wnt1Cre:R26R</i> cardiosensor lines. <i>Mlc1v-nlacZ24</i> (A, D, G), <i>Hoxa3-lacZ</i> (B, E, H) and Wnt1<i>Cre:R26R</i> (C, F, I).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-11-16 00:54:17"}
  • {"files"=>["https://ndownloader.figshare.com/files/712370"], "description"=>"<p>Transverse (A, C, E) and sagittal (B, D, F) sections of E9.5 and E10.5 transgenic embryos, respectively. (A) The X-gal-stained cells in the outer layer of the outflow tract also express Isl1 (C, arrowheads). Only transgenic cells in the distal outflow tract (delineated by arrowheads in A) express c-actin (E, arrowheads). Note that dorsal part of the developing aortic sac (asterisk in A) is filled with Isl1-expressing cells. (B) X-gal-stained cells are present in the outflow tract region and anterior portion of SHF demarcated by the red arrowheads. (D) Isl1-immunostained cells are present in this anterior portion of SHF (demarcated by the red arrowheads) and outflow myocardium, whereas c-actin is restricted to myocardial transgenic cells of the outflow tract (F, arrowheads). OFT, outflow tract. Black and white asterisks in (A) and (B) show the aortic sac and pharyngeal endoderm, respectively. Scale bars = 100 µm.</p>", "links"=>[], "tags"=>["islet-1", "cardiac", "actin", "hearts", "transgenic", "embryos"], "article_id"=>382724, "categories"=>["Molecular Biology", "Biotechnology", "Developmental Biology", "Biological Sciences"], "users"=>["Nata Y. S.-G. Diman", "Sophie Remacle", "Nicolas Bertrand", "Jacques J. Picard", "Stéphane Zaffran", "René Rezsohazy"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0027624.g005", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_946_gal_Islet_1_and_cardiac_actin_expression_in_the_hearts_of_transgenic_embryos_at_E9_5_and_E10_5_/382724", "title"=>"β-gal, Islet-1 and cardiac actin expression in the hearts of transgenic embryos at E9.5 and E10.5.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-11-16 00:45:24"}
  • {"files"=>["https://ndownloader.figshare.com/files/711921"], "description"=>"<p>Sagittal sections of E10.5 transgenic embryos at the level of the 3<sup>rd</sup>, 4<sup>th</sup> and 6<sup>th</sup> pharyngeal arches (A, B), aortic sac (C, D), and outflow tract (E, F). Ap2α (B, D) and N-cadherin (F) immunochemistry on X-gal-stained adjacent sections demonstrates that <i>lacZ</i>-positive cells in the pharyngeal arches (B) as well as those populating the space between the pharyngeal endoderm and aortic sac (D) and outflow tract mesenchyme (F) are cardiac neural crest cells. Arrowheads and arrows (B) indicate the pharyngeal ectoderm and AP2<sup>+</sup> LacZ<sup>+</sup> cells in pharyngeal arches ectoderm and mesoderm, respectively. Arrows and arrowheads (C, D) show the pharyngeal endoderm and AP2<sup>+</sup> LacZ<sup>+</sup> cells, respectively. AS, aortic sac; OFT, outflow tract. RA, right atrium 3, 4, and 6 indicate the 3<sup>rd</sup>, 4<sup>th</sup> and 6<sup>th</sup> pharyngeal arches, respectively. Scale bars = 100 µm.</p>", "links"=>[], "tags"=>["cells", "pharyngeal", "aortic", "sac", "oft", "migratory", "neural", "crest"], "article_id"=>382268, "categories"=>["Molecular Biology", "Biotechnology", "Developmental Biology", "Biological Sciences"], "users"=>["Nata Y. S.-G. Diman", "Sophie Remacle", "Nicolas Bertrand", "Jacques J. Picard", "Stéphane Zaffran", "René Rezsohazy"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0027624.g003", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_lacZ_positive_cells_in_the_pharyngeal_arches_aortic_sac_and_OFT_identify_migratory_neural_crest_cells_/382268", "title"=>"<i>lacZ</i>-positive cells in the pharyngeal arches, aortic sac and OFT identify migratory neural crest cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-11-16 00:37:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/712653"], "description"=>"<p>Lateral views of E8.5 (A, B), E9.5 (C, D), and frontal views of E8 (E,F,G) embryos. In contrast to control <i>Hoxa3-lacZ</i> embryos (A, C), ß-galactosidase activity is lost in <i>Hoxa3-lacZ</i>/ <i>Raldh2−/−</i> embryos (B, D). <i>Hoxa3–lacZ</i> embryos treated with vehicle (DMSO) show no activation of the transgene (E) whereas RA-treated transgenic embryos display a precocious ß-galactosidase upregulation in the SHF (F, G, arrowheads). Arrows (F, G) indicate the cardiac crescent. Asterisk indicates the otic vesicle. HT, heart tube. Scale bars = 100 µm.</p>", "links"=>[], "tags"=>["signaling", "affects", "transgenic"], "article_id"=>383018, "categories"=>["Molecular Biology", "Biotechnology", "Developmental Biology", "Biological Sciences"], "users"=>["Nata Y. S.-G. Diman", "Sophie Remacle", "Nicolas Bertrand", "Jacques J. Picard", "Stéphane Zaffran", "René Rezsohazy"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0027624.g006", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_RA_signaling_affects_Hoxa3_lacZ_expression_in_transgenic_embryos_/383018", "title"=>"RA signaling affects <i>Hoxa3-lacZ</i> expression in transgenic embryos.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-11-16 00:50:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/361094", "https://ndownloader.figshare.com/files/361169"], "description"=>"<div><p>A transgenic mouse line harbouring a β-galacdosidase reporter gene controlled by the proximal 2 kb promoter of <em>Hoxa3</em> was previously generated to investigate the regulatory cues governing <em>Hoxa3</em> expression in the mouse. Examination of transgenic embryos from embryonic day (E) 8.0 to E15.5 revealed regionally restricted reporter activity in the developing heart. Indeed, transgene expression specifically delineated cells from three distinct lineages: a subpopulation of the second heart field contributing to outflow tract myocardium, the cardiac neural crest cells and the pharyngeal endoderm. Manipulation of the Retinoic Acid (RA) signaling pathway showed that RA is required for correct expression of the transgene. Therefore, this transgenic line may serve as a cardiosensor line of particular interest for further analysis of outflow tract development.</p> </div>", "links"=>[], "tags"=>["retinoic", "responsive", "transgene", "expressed", "embryonic", "pharyngeal", "cardiac", "neural", "crest", "subdomain"], "article_id"=>131391, "categories"=>["Molecular Biology", "Biotechnology", "Developmental Biology", "Biological Sciences"], "users"=>["Nata Y. S.-G. Diman", "Sophie Remacle", "Nicolas Bertrand", "Jacques J. Picard", "Stéphane Zaffran", "René Rezsohazy"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0027624.s001", "https://dx.doi.org/10.1371/journal.pone.0027624.s002"], "stats"=>{"downloads"=>2, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/A_Retinoic_Acid_Responsive_Hoxa3_Transgene_Expressed_in_Embryonic_Pharyngeal_Endoderm_Cardiac_Neural_Crest_and_a_Subdomain_of_the_Second_Heart_Field/131391", "title"=>"A Retinoic Acid Responsive <em>Hoxa3</em> Transgene Expressed in Embryonic Pharyngeal Endoderm, Cardiac Neural Crest and a Subdomain of the Second Heart Field", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2011-11-16 00:23:11"}

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