Extracting Fluorescent Reporter Time Courses of Cell Lineages from High-Throughput Microscopy at Low Temporal Resolution
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Extracting fluorescent reporter time courses of cell lineages from high-throughput microscopy at low temporal resolution", "type"=>"journal", "authors"=>[{"first_name"=>"Mike J.", "last_name"=>"Downey", "scopus_author_id"=>"54388805900"}, {"first_name"=>"Danuta M.", "last_name"=>"Jeziorska", "scopus_author_id"=>"33467624200"}, {"first_name"=>"Sascha", "last_name"=>"Ott", "scopus_author_id"=>"7102819225"}, {"first_name"=>"T. Katherine", "last_name"=>"Tamai", "scopus_author_id"=>"7102292144"}, {"first_name"=>"Georgy", "last_name"=>"Koentges", "scopus_author_id"=>"6603013392"}, {"first_name"=>"Keith W.", "last_name"=>"Vance", "scopus_author_id"=>"6603541491"}, {"first_name"=>"Till", "last_name"=>"Bretschneider", "scopus_author_id"=>"7003316282"}], "year"=>2011, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-83455231496", "pui"=>"363090434", "doi"=>"10.1371/journal.pone.0027886", "sgr"=>"83455231496", "pmid"=>"22194797"}, "id"=>"ddcb79bc-b4e0-3166-96b9-362ba06b1a78", "abstract"=>"The extraction of fluorescence time course data is a major bottleneck in high-throughput live-cell microscopy. Here we present an extendible framework based on the open-source image analysis software ImageJ, which aims in particular at analyzing the expression of fluorescent reporters through cell divisions. The ability to track individual cell lineages is essential for the analysis of gene regulatory factors involved in the control of cell fate and identity decisions. In our approach, cell nuclei are identified using Hoechst, and a characteristic drop in Hoechst fluorescence helps to detect dividing cells. We first compare the efficiency and accuracy of different segmentation methods and then present a statistical scoring algorithm for cell tracking, which draws on the combination of various features, such as nuclear intensity, area or shape, and importantly, dynamic changes thereof. Principal component analysis is used to determine the most significant features, and a global parameter search is performed to determine the weighting of individual features. Our algorithm has been optimized to cope with large cell movements, and we were able to semi-automatically extract cell trajectories across three cell generations. Based on the MTrackJ plugin for ImageJ, we have developed tools to efficiently validate tracks and manually correct them by connecting broken trajectories and reassigning falsely connected cell positions. A gold standard consisting of two time-series with 15,000 validated positions will be released as a valuable resource for benchmarking. We demonstrate how our method can be applied to analyze fluorescence distributions generated from mouse stem cells transfected with reporter constructs containing transcriptional control elements of the Msx1 gene, a regulator of pluripotency, in mother and daughter cells. Furthermore, we show by tracking zebrafish PAC2 cells expressing FUCCI cell cycle markers, our framework can be easily adapted to different cell types and fluorescent markers.", "link"=>"http://www.mendeley.com/research/extracting-fluorescent-reporter-time-courses-cell-lineages-highthroughput-microscopy-low-temporal-re", "reader_count"=>60, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>7, "Researcher"=>16, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>16, "Student > Postgraduate"=>2, "Student > Master"=>12, "Student > Bachelor"=>2, "Lecturer > Senior Lecturer"=>1, "Professor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>7, "Researcher"=>16, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>16, "Student > Postgraduate"=>2, "Student > Master"=>12, "Student > Bachelor"=>2, "Lecturer > Senior Lecturer"=>1, "Professor"=>2}, "reader_count_by_subject_area"=>{"Engineering"=>4, "Unspecified"=>4, "Biochemistry, Genetics and Molecular Biology"=>6, "Materials Science"=>1, "Mathematics"=>1, "Agricultural and Biological Sciences"=>27, "Medicine and Dentistry"=>4, "Chemical Engineering"=>1, "Physics and Astronomy"=>3, "Computer Science"=>9}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>4}, "Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Physics and Astronomy"=>{"Physics and Astronomy"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>27}, "Computer Science"=>{"Computer Science"=>9}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}, "Mathematics"=>{"Mathematics"=>1}, "Unspecified"=>{"Unspecified"=>4}, "Chemical Engineering"=>{"Chemical Engineering"=>1}}, "reader_count_by_country"=>{"Belgium"=>1, "Japan"=>2, "United Kingdom"=>1, "Portugal"=>2, "Germany"=>1}, "group_count"=>3}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/83455231496"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/83455231496?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=83455231496&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=83455231496&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/83455231496", "dc:identifier"=>"SCOPUS_ID:83455231496", "eid"=>"2-s2.0-83455231496", "dc:title"=>"Extracting fluorescent reporter time courses of cell lineages from high-throughput microscopy at low temporal resolution", "dc:creator"=>"Downey M.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"6", "prism:issueIdentifier"=>"12", "prism:pageRange"=>nil, "prism:coverDate"=>"2011-12-15", "prism:coverDisplayDate"=>"15 December 2011", "prism:doi"=>"10.1371/journal.pone.0027886", "citedby-count"=>"17", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"The University of Warwick", "affiliation-city"=>"Coventry", "affiliation-country"=>"United Kingdom"}], "pubmed-id"=>"22194797", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e27886", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0027886", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"55", "xml_views"=>"16", "html_views"=>"321"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"75", "xml_views"=>"36", "html_views"=>"116"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"43", "xml_views"=>"20", "html_views"=>"72"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"28", "xml_views"=>"9", "html_views"=>"69"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"33", "xml_views"=>"17", "html_views"=>"55"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"72"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"95"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"11", "xml_views"=>"5", "html_views"=>"42"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"72"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"98"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"75"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"75"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"71"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"71"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"93"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"105"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"114"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"106"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"115"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"91"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"3", "html_views"=>"99"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"75"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"74"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"100"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"104"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"67"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"88"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"54"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"15", "xml_views"=>"2", "html_views"=>"63"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"41"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"37"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"49"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"48"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"2", "html_views"=>"45"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"29"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"3", "html_views"=>"34"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"48"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"8"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"2", "html_views"=>"17"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"3", "html_views"=>"3"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"3"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/701513"], "description"=>"<p>Horizontal axis shows time with the vertical axis representing cells in the frames. The red line is the manually tracked ‘gold standard’ route marked through the cells, and the black line is the calculated tracking. Tracking accuracy is measured by counting the total number of steps which match the gold standard and the longest continuous chain of correct steps.</p>", "links"=>[], "tags"=>["tracking"], "article_id"=>371885, "categories"=>["Molecular Biology", "Cell Biology", "Genetics"], "users"=>["Mike J. Downey", "Danuta M. Jeziorska", "Sascha Ott", "T. Katherine Tamai", "Georgy Koentges", "Keith W. Vance", "Till Bretschneider"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0027886.g004", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Measuring_tracking_accuracy_/371885", "title"=>"Measuring tracking accuracy.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-12-15 00:31:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/701882"], "description"=>"<p>Fluorescence intensity following cell division for the two daughter cells in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0027886#pone-0027886-g006\" target=\"_blank\">figure 6</a>. The two FUCCI channels have been shown for an entire cell cycle. The G1 signal (red) increases gradually following mitosis, then decreases following a rise in S-G2-M signal (green). A magnified view of the first 3 hours is shown in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0027886#pone.0027886.s011\" target=\"_blank\">Figures S11</a> and <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0027886#pone.0027886.s012\" target=\"_blank\">S12</a>.</p>", "links"=>[], "tags"=>["fucci", "markers"], "article_id"=>372251, "categories"=>["Molecular Biology", "Cell Biology", "Genetics"], "users"=>["Mike J. Downey", "Danuta M. Jeziorska", "Sascha Ott", "T. Katherine Tamai", "Georgy Koentges", "Keith W. Vance", "Till Bretschneider"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0027886.g007", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Intensities_of_the_FUCCI_markers_following_cell_division_/372251", "title"=>"Intensities of the FUCCI markers following cell division.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-12-15 00:37:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/701568"], "description"=>"<p>A) Correlation plots of daughter fluorescence (R<sup>2</sup> = 0.92) taken from the 5 Msx1 ReMo constructs. B) Sum of daughter fluorescence and difference between daughter fluorescence, as a percentage of parent fluorescence. C) Breakdown of sum and difference of intensities for the 5 different Msx1 ReMo constructs.</p>", "links"=>[], "tags"=>["fluorescence"], "article_id"=>371949, "categories"=>["Molecular Biology", "Cell Biology", "Genetics"], "users"=>["Mike J. Downey", "Danuta M. Jeziorska", "Sascha Ott", "T. Katherine Tamai", "Georgy Koentges", "Keith W. Vance", "Till Bretschneider"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0027886.g005", "stats"=>{"downloads"=>3, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_GFP_Fluorescence_measurements_across_cell_divisions_/371949", "title"=>"GFP Fluorescence measurements across cell divisions.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-12-15 00:32:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/701354"], "description"=>"<p>Tracked cells are plotted in blue. Cells that divided between consecutive frames are plotted as red circles. R<sup>2</sup> values are given only for very highly correlated values. A) Integrated Hoechst intensity. Non-dividing cells show a very high correlation in Hoechst between frames (blue R<sup>2</sup> = 0.97). Red cells show that Hoechst levels are halved during division (red R<sup>2</sup> = 0.90). B) Mean Hoechst intensity (blue R<sup>2</sup> = 0.94). C) Change in Integrated Hoechst. D) Nucleus area. (blue R<sup>2</sup> = 0.84). E) Change in nucleus area. F) 2nd Intensity moment (measured on Hoechst channel, blue R<sup>2</sup> = 0.85).</p>", "links"=>[], "tags"=>["genetics and genomics", "molecular biology", "cell biology"], "article_id"=>371729, "categories"=>["Molecular Biology", "Cell Biology", "Genetics"], "users"=>["Mike J. Downey", "Danuta M. Jeziorska", "Sascha Ott", "T. Katherine Tamai", "Georgy Koentges", "Keith W. Vance", "Till Bretschneider"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0027886.g003", "stats"=>{"downloads"=>7, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Correlations_of_different_features_between_consecutive_frames_/371729", "title"=>"Correlations of different features between consecutive frames.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-12-15 00:28:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/701257"], "description"=>"<p>A) Original nuclei (scale bar 50 microns) taken from the gold standard data set, cell density 1150 cells/mm<sup>2</sup>. B–H) Nuclei with segmentation examples overlaid. Ellipses indicate segmentation errors. Lines indicate unresolved clusters of cells. B) Manually marked cell position. C) Cellomics segmentation. D) Seeded Growth. E) Global Threshold. F) Local Threshold. G) Scaling Index. H) CellProfiler. I) Cell detection accuracy measurements: Total cell count, false negatives and false positives comparing different segmentation methods to the gold standard.</p>", "links"=>[], "tags"=>["genetics and genomics", "molecular biology", "cell biology"], "article_id"=>371632, "categories"=>["Molecular Biology", "Cell Biology", "Genetics"], "users"=>["Mike J. Downey", "Danuta M. Jeziorska", "Sascha Ott", "T. Katherine Tamai", "Georgy Koentges", "Keith W. Vance", "Till Bretschneider"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0027886.g002", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Segmentation_of_cell_nuclei_/371632", "title"=>"Segmentation of cell nuclei.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-12-15 00:27:12"}
  • {"files"=>["https://ndownloader.figshare.com/files/702051"], "description"=>"<p>Principal Component Analysis was used to determine which features contributed most to the tracking accuracy. The cumulative components columns specify how much variance of each feature is described by the first 3 principal components. Features in bold are used in the tracking system.</p>‡<p>Derived from other features. R<sup>2</sup> values are given for non-dividing cells only.</p>", "links"=>[], "tags"=>["derived"], "article_id"=>372427, "categories"=>["Molecular Biology", "Cell Biology", "Genetics"], "users"=>["Mike J. Downey", "Danuta M. Jeziorska", "Sascha Ott", "T. Katherine Tamai", "Georgy Koentges", "Keith W. Vance", "Till Bretschneider"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0027886.t001", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Measured_and_derived_features_used_in_tracking_/372427", "title"=>"Measured and derived features used in tracking.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2011-12-15 00:40:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/702017"], "description"=>"<p>Two numbers are given for each measurement: total number of correctly tracked steps and longest continuously tracked section (as percentage of total steps). For the 10 minute interval experiment, the seeded growth algorithm was used, and segmentations were manually edited, so that 50% of cells with positively validated segmentations were included in the tracking gold standard. The 30 minute interval experiment is based on the Cellomics segmentation, as to allow comparison of the Cellomics tracking routines with other ones.</p><p>*CellProfiler tracking using LAP (Linear Assignment Problem) tracking.</p>", "links"=>[], "tags"=>["tracked"], "article_id"=>372394, "categories"=>["Molecular Biology", "Cell Biology", "Genetics"], "users"=>["Mike J. Downey", "Danuta M. Jeziorska", "Sascha Ott", "T. Katherine Tamai", "Georgy Koentges", "Keith W. Vance", "Till Bretschneider"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0027886.t002", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Results_of_gold_standard_tracked_sets_/372394", "title"=>"Results of gold standard tracked sets.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2011-12-15 00:39:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/701786"], "description"=>"<p>Time is in minutes following division. The overlap in the red and green fluorescence (transition between G1 and S phase) is shown for cell b (bottom panel). White outlines are given for nuclei showing weak fluorescence.</p>", "links"=>[], "tags"=>["changes", "indicated", "fucci", "markers", "cells", "labelled"], "article_id"=>372151, "categories"=>["Molecular Biology", "Cell Biology", "Genetics"], "users"=>["Mike J. Downey", "Danuta M. Jeziorska", "Sascha Ott", "T. Katherine Tamai", "Georgy Koentges", "Keith W. Vance", "Till Bretschneider"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0027886.g006", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Colour_changes_during_the_cell_cycle_indicated_by_FUCCI_markers_in_two_daughter_cells_labelled_a_and_b_see_also_Figure_7_/372151", "title"=>"Colour changes during the cell cycle indicated by FUCCI markers in two daughter cells labelled a and b (see also <b>Figure 7</b>).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-12-15 00:35:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/701120"], "description"=>"<p>A) C2C12 cells labelled with Hoechst stain. B) Same view showing expression of GFP driven by a Msx1 promoter. GFP expressing cells have been highlighted in yellow in A and B. C) Potential ambiguity in linking cells in subsequent frames (white outlines). Arrows represent potential trajectory assignments with numbers representing the calculated score for each potential assignment. D and E) Cell divisions exhibiting chromatin condensation close to the point of division. Time is displayed in minutes. Scale bar in all images is 50 microns. (C and D have been adapted from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0027886#pone.0027886-Downey1\" target=\"_blank\">[24]</a>, © 2011 IEEE).</p>", "links"=>[], "tags"=>["cellomics", "automated"], "article_id"=>371493, "categories"=>["Molecular Biology", "Cell Biology", "Genetics"], "users"=>["Mike J. Downey", "Danuta M. Jeziorska", "Sascha Ott", "T. Katherine Tamai", "Georgy Koentges", "Keith W. Vance", "Till Bretschneider"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0027886.g001", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Magnified_section_of_an_image_obtained_from_the_Cellomics_automated_microscope_/371493", "title"=>"Magnified section of an image obtained from the Cellomics automated microscope.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-12-15 00:24:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/356695", "https://ndownloader.figshare.com/files/356734", "https://ndownloader.figshare.com/files/356783", "https://ndownloader.figshare.com/files/356847", "https://ndownloader.figshare.com/files/356895", "https://ndownloader.figshare.com/files/356947", "https://ndownloader.figshare.com/files/356984", "https://ndownloader.figshare.com/files/357025", "https://ndownloader.figshare.com/files/357045", "https://ndownloader.figshare.com/files/357091", "https://ndownloader.figshare.com/files/357114", "https://ndownloader.figshare.com/files/357162", "https://ndownloader.figshare.com/files/357223", "https://ndownloader.figshare.com/files/357263", "https://ndownloader.figshare.com/files/357312", "https://ndownloader.figshare.com/files/357357", "https://ndownloader.figshare.com/files/357430", "https://ndownloader.figshare.com/files/357492"], "description"=>"<div><p>The extraction of fluorescence time course data is a major bottleneck in high-throughput live-cell microscopy. Here we present an extendible framework based on the open-source image analysis software ImageJ, which aims in particular at analyzing the expression of fluorescent reporters through cell divisions. The ability to track individual cell lineages is essential for the analysis of gene regulatory factors involved in the control of cell fate and identity decisions. In our approach, cell nuclei are identified using Hoechst, and a characteristic drop in Hoechst fluorescence helps to detect dividing cells. We first compare the efficiency and accuracy of different segmentation methods and then present a statistical scoring algorithm for cell tracking, which draws on the combination of various features, such as nuclear intensity, area or shape, and importantly, dynamic changes thereof. Principal component analysis is used to determine the most significant features, and a global parameter search is performed to determine the weighting of individual features. Our algorithm has been optimized to cope with large cell movements, and we were able to semi-automatically extract cell trajectories across three cell generations. Based on the MTrackJ plugin for ImageJ, we have developed tools to efficiently validate tracks and manually correct them by connecting broken trajectories and reassigning falsely connected cell positions. A gold standard consisting of two time-series with 15,000 validated positions will be released as a valuable resource for benchmarking. We demonstrate how our method can be applied to analyze fluorescence distributions generated from mouse stem cells transfected with reporter constructs containing transcriptional control elements of the Msx1 gene, a regulator of pluripotency, in mother and daughter cells. Furthermore, we show by tracking zebrafish PAC2 cells expressing FUCCI cell cycle markers, our framework can be easily adapted to different cell types and fluorescent markers.</p> </div>", "links"=>[], "tags"=>["extracting", "fluorescent", "courses", "lineages", "high-throughput", "microscopy", "temporal"], "article_id"=>130483, "categories"=>["Molecular Biology", "Cell Biology", "Genetics"], "users"=>["Mike J. Downey", "Danuta M. Jeziorska", "Sascha Ott", "T. Katherine Tamai", "Georgy Koentges", "Keith W. Vance", "Till Bretschneider"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0027886.s001", "https://dx.doi.org/10.1371/journal.pone.0027886.s002", "https://dx.doi.org/10.1371/journal.pone.0027886.s003", "https://dx.doi.org/10.1371/journal.pone.0027886.s004", "https://dx.doi.org/10.1371/journal.pone.0027886.s005", "https://dx.doi.org/10.1371/journal.pone.0027886.s006", "https://dx.doi.org/10.1371/journal.pone.0027886.s007", "https://dx.doi.org/10.1371/journal.pone.0027886.s008", "https://dx.doi.org/10.1371/journal.pone.0027886.s009", "https://dx.doi.org/10.1371/journal.pone.0027886.s010", "https://dx.doi.org/10.1371/journal.pone.0027886.s011", "https://dx.doi.org/10.1371/journal.pone.0027886.s012", "https://dx.doi.org/10.1371/journal.pone.0027886.s013", "https://dx.doi.org/10.1371/journal.pone.0027886.s014", "https://dx.doi.org/10.1371/journal.pone.0027886.s015", "https://dx.doi.org/10.1371/journal.pone.0027886.s016", "https://dx.doi.org/10.1371/journal.pone.0027886.s017", "https://dx.doi.org/10.1371/journal.pone.0027886.s018"], "stats"=>{"downloads"=>44, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Extracting_Fluorescent_Reporter_Time_Courses_of_Cell_Lineages_from_High_Throughput_Microscopy_at_Low_Temporal_Resolution/130483", "title"=>"Extracting Fluorescent Reporter Time Courses of Cell Lineages from High-Throughput Microscopy at Low Temporal Resolution", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2011-12-15 00:08:03"}

PMC Usage Stats | Further Information

  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"11", "year"=>"2011", "pdf"=>"5", "unique-ip"=>"10", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"29", "unique-ip"=>"33", "pdf"=>"7", "year"=>"2012", "figure"=>"16", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"29", "year"=>"2012", "pdf"=>"21", "unique-ip"=>"30", "figure"=>"10", "scanned-summary"=>"0", "supp-data"=>"3"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"17", "unique-ip"=>"20", "pdf"=>"8", "year"=>"2012", "figure"=>"10", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"10", "year"=>"2012", "pdf"=>"9", "unique-ip"=>"12", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"8", "unique-ip"=>"9", "pdf"=>"3", "year"=>"2012", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"10", "year"=>"2012", "pdf"=>"5", "unique-ip"=>"13", "figure"=>"18", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"22", "full-text"=>"23", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"27", "full-text"=>"20", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"0", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"12", "full-text"=>"15", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"7", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"15", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"20", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"3", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"18", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"2", "pdf"=>"2", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"22", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"19", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"16", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"15", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"18", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"18", "full-text"=>"11", "pdf"=>"9", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"19", "full-text"=>"24", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"16", "full-text"=>"12", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"14", "full-text"=>"9", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2011-01-01T00:00:00Z", "end_date"=>"2011-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Organisms", "average_usage"=>[316, 571, 699, 812, 915, 1008, 1095, 1180, 1255, 1319, 1393, 1461, 1528, 1595, 1662, 1729, 1802, 1868, 1937, 1997, 2062, 2130, 2192, 2254, 2324, 2386, 2450, 2510, 2579, 2642, 2716, 2778, 2842, 2908, 2979, 3042, 3095]}, {"subject_area"=>"/Physical sciences/Mathematics", "average_usage"=>[347, 582, 700, 805, 909, 997, 1061, 1147, 1220, 1282, 1350, 1411, 1471, 1532, 1600, 1665, 1726, 1790, 1868, 1927, 1986, 2048, 2105, 2166, 2229, 2288, 2347, 2407, 2463, 2525, 2589, 2650, 2704, 2755, 2806, 2854, 2896]}]}
Loading … Spinner
There are currently no alerts