Successful Cognitive Aging in Rats: A Role for mGluR5 Glutamate Receptors, Homer 1 Proteins and Downstream Signaling Pathways
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Successful cognitive aging in rats: A role for mGluR5 glutamate receptors, homer 1 proteins and downstream signaling pathways", "type"=>"journal", "authors"=>[{"first_name"=>"Caroline", "last_name"=>"Ménard", "scopus_author_id"=>"8054406600"}, {"first_name"=>"Rémi", "last_name"=>"Quirion", "scopus_author_id"=>"56031971000"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"22238580", "doi"=>"10.1371/journal.pone.0028666", "sgr"=>"84855433975", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "scopus"=>"2-s2.0-84855433975", "issn"=>"19326203", "pui"=>"364045375"}, "id"=>"add84958-ef7b-31b0-ba60-c4a5b9bfe67b", "abstract"=>"Normal aging is associated with impairments in cognition, especially learning and memory. However, major individual differences are known to exist. Using the classical Morris Water Maze (MWM) task, we discriminated a population of 24-months old Long Evans aged rats in two groups--memory-impaired (AI) and memory-unimpaired (AU) in comparison with 6-months old adult animals. AI rats presented deficits in learning, reverse memory and retention. At the molecular level, an increase in metabotropic glutamate receptors 5 (mGluR5) was observed in post-synaptic densities (PSD) in the hippocampus of AU rats after training. Scaffolding Homer 1b/c proteins binding to group 1 mGluR facilitate coupling with its signaling effectors while Homer 1a reduces it. Both Homer 1a and 1b/c levels were up-regulated in the hippocampus PSD of AU animals following MWM task. Using immunohistochemistry we further demonstrated that mGluR5 as well as Homer 1b/c stainings were enhanced in the CA1 hippocampus sub-field of AU animals. In fact mGluR5 and Homer 1 isoforms were more abundant and co-localized in the hippocampal dendrites in AU rats. However, the ratio of Homer 1a/Homer 1b/c bound to mGluR5 in the PSD was four times lower for AU animals compared to AI rats. Consequently, AU animals presented higher PKCγ, ERK, p70S6K, mTOR and CREB activation. Finally the expression of immediate early gene Arc/Arg3.1 was shown to be higher in AU rats in accordance with its role in spatial memory consolidation. On the basis of these results, a model of successful cognitive aging with a critical role for mGluR5, Homer 1 proteins and downstream signalling pathways is proposed here.", "link"=>"http://www.mendeley.com/research/successful-cognitive-aging-rats-role-mglur5-glutamate-receptors-homer-1-proteins-downstream-signalin", "reader_count"=>91, "reader_count_by_academic_status"=>{"Unspecified"=>3, "Professor > Associate Professor"=>3, "Librarian"=>1, "Student > Doctoral Student"=>3, "Researcher"=>21, "Student > Ph. D. Student"=>36, "Student > Postgraduate"=>2, "Student > Master"=>10, "Other"=>2, "Student > Bachelor"=>6, "Lecturer"=>1, "Professor"=>3}, "reader_count_by_user_role"=>{"Unspecified"=>3, "Professor > Associate Professor"=>3, "Librarian"=>1, "Student > Doctoral Student"=>3, "Researcher"=>21, "Student > Ph. D. Student"=>36, "Student > Postgraduate"=>2, "Student > Master"=>10, "Other"=>2, "Student > Bachelor"=>6, "Lecturer"=>1, "Professor"=>3}, "reader_count_by_subject_area"=>{"Unspecified"=>4, "Biochemistry, Genetics and Molecular Biology"=>1, "Medicine and Dentistry"=>13, "Agricultural and Biological Sciences"=>49, "Neuroscience"=>19, "Sports and Recreations"=>3, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Psychology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>13}, "Neuroscience"=>{"Neuroscience"=>19}, "Sports and Recreations"=>{"Sports and Recreations"=>3}, "Psychology"=>{"Psychology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>49}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>4}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"Hungary"=>1, "United States"=>1, "United Kingdom"=>2, "Chile"=>1, "Germany"=>1, "India"=>1}, "group_count"=>5}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84855433975"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84855433975?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84855433975&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84855433975&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84855433975", "dc:identifier"=>"SCOPUS_ID:84855433975", "eid"=>"2-s2.0-84855433975", "dc:title"=>"Successful cognitive aging in rats: A role for mGluR5 glutamate receptors, homer 1 proteins and downstream signaling pathways", "dc:creator"=>"Ménard C.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"7", "prism:issueIdentifier"=>"1", "prism:pageRange"=>nil, "prism:coverDate"=>"2012-01-06", "prism:coverDisplayDate"=>"6 January 2012", "prism:doi"=>"10.1371/journal.pone.0028666", "citedby-count"=>"62", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"McGill University, Douglas Mental Health University Institute", "affiliation-city"=>"Montreal", "affiliation-country"=>"Canada"}], "pubmed-id"=>"22238580", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e28666", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0028666", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"188", "xml_views"=>"46", "html_views"=>"980"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"68", "xml_views"=>"20", "html_views"=>"93"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"46", "xml_views"=>"8", "html_views"=>"105"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"31", "xml_views"=>"20", "html_views"=>"52"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"33", "xml_views"=>"0", "html_views"=>"79"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"30", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"32", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"20", "xml_views"=>"5", "html_views"=>"70"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"32", "xml_views"=>"0", "html_views"=>"89"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"33", "xml_views"=>"0", "html_views"=>"123"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"40", "xml_views"=>"0", "html_views"=>"168"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"78"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"110"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"14", "xml_views"=>"2", "html_views"=>"82"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"71"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"16", "xml_views"=>"2", "html_views"=>"66"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"121"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"62"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"24", "xml_views"=>"2", "html_views"=>"216"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"154"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"106"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"127"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"2", "html_views"=>"169"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"164"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"212"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"192"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"134"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"19", "xml_views"=>"2", "html_views"=>"97"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"44"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"4", "html_views"=>"135"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"30", "xml_views"=>"1", "html_views"=>"93"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"2", "html_views"=>"40"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"45"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"39"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"35"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"46"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"37"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"2", "html_views"=>"66"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"3", "html_views"=>"24"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"3", "html_views"=>"19"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"2", "html_views"=>"25"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"10"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/694691"], "description"=>"<p>A) mGluR5 protein level was decreased in the hippocampal homogenates of AI animals and B) this modification was correlated to the MWM performance for aged rats. C) mGluR1α receptor level in the PSD was decreased for AI rats while AU animals showed an increase in mGluR5 level. D) Both alterations were correlated to the MWM day 5 latencies for aged rats. E) mGluR5 immunostaining was strongly reduced in the AI hippocampus particularly in the dendrites of the CA1 region. AU displayed small increase in mGluR5 level in CA1 and DG. Western Blot values represent mean ± SEM of three separate experiments (N = 6–9) and the data are expressed as percentage of control (6-months adult rats), *<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001.</p>", "links"=>[], "tags"=>["mglur", "levels", "ai", "au", "hippocampal"], "article_id"=>365120, "categories"=>["Molecular Biology", "Biological Sciences", "Neuroscience", "Physiology"], "users"=>["Caroline Ménard", "Rémi Quirion"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0028666.g004", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparison_of_group_1_mGluR_levels_in_adult_Y_AI_and_AU_hippocampal_formations_/365120", "title"=>"Comparison of group 1 mGluR levels in adult (Y), AI and AU hippocampal formations.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-01-06 01:25:20"}
  • {"files"=>["https://ndownloader.figshare.com/files/695428"], "description"=>"<p>MWM training induces release of glutamate which then activates group 1 mGluRs on the plasma membrane (1). Metabotropic mGluR5 are linked to ionotropic glutamate receptors by multiple scaffolding proteins (Homer 1 b/c, Shank, GKAP, PSD95). Following mGluR activation, protein kinases PKCγ, ERK1/2, mTOR, p70S6K and CREB become phosphorylated (2) and induce IEG expression (3). Homer 1a is released in the cytosol and translocated to plasma membrane (4) uncoupling the receptors with its signaling effectors (5). High levels of mGluR5 receptors bound to Homer long isoforms in the PSD of the AU rats maintain the activation of crucial signaling pathways and IEG expression following spatial memory training.</p>", "links"=>[], "tags"=>["mechanisms", "activated", "aged", "memory-unimpaired", "psd", "mwm"], "article_id"=>365851, "categories"=>["Molecular Biology", "Biological Sciences", "Neuroscience", "Physiology"], "users"=>["Caroline Ménard", "Rémi Quirion"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0028666.g010", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Possible_mechanisms_activated_in_the_aged_memory_unimpaired_PSD_following_MWM_training_/365851", "title"=>"Possible mechanisms activated in the aged memory-unimpaired PSD following MWM training.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-01-06 01:37:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/694916"], "description"=>"<p>A) Homer 1a immunofluorescence and co-localization with mGluR5 was overall stronger in the cell bodies and dendrites of CA1 area in AU animals. At higher magnification (bottom), both mGluR5 and Homer 1a appear to be expressed more in the dendrites of AU animals but the co-localization seems to be similar for both AI and AU rats. B) mGluR5 and Homer 1 b/c co-localization was also overall higher in the cell bodies and dendrites of the CA1 region in AU animals. Both mGluR5 and Homer 1b/c are more expressed and co-localized in the AU dendrites compared to AI (bottom).</p>", "links"=>[], "tags"=>["mglur5", "homer"], "article_id"=>365356, "categories"=>["Molecular Biology", "Biological Sciences", "Neuroscience", "Physiology"], "users"=>["Caroline Ménard", "Rémi Quirion"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0028666.g006", "stats"=>{"downloads"=>1, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Co_localization_of_mGluR5_and_Homer_1_isoforms_/365356", "title"=>"Co-localization of mGluR5 and Homer 1 isoforms.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-01-06 01:29:16"}
  • {"files"=>["https://ndownloader.figshare.com/files/694519"], "description"=>"<p>A) Learning curves and representative tracking pictures for week 2 as well as average results for the reverse probe test on day 11 (probe 2). B) Swim speed distribution in all groups during probe 2 test. C) Time spent in target and opposite quadrants during the reverse probe test. D) Results of the retention probe test conducted one week after the reverse probe test. Representative tracking pictures for AI and AU groups are presented. E) Time spent in learning and reverse platform quadrants during the retention probe test. Data represent mean ± SEM for all animals of each group (N = 26–32), *<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001.</p>", "links"=>[], "tags"=>["aging"], "article_id"=>364954, "categories"=>["Molecular Biology", "Biological Sciences", "Neuroscience", "Physiology"], "users"=>["Caroline Ménard", "Rémi Quirion"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0028666.g002", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_aging_on_reverse_memory_and_retention_/364954", "title"=>"Effect of aging on reverse memory and retention.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-01-06 01:22:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/694595"], "description"=>"<p>A) Distribution of day 5 latency for all 3 groups; this parameter is used to correlate protein levels with MWM performance. B) Number of platform crossings distribution during learning and reverse probe tests (mean of probes 1 and 2) for all groups. C) Day 5 latency correlation with the mean of learning and reverse probe test results for both adult and aged rats. D) Correlation of day 5 latency with the mean of time spent in target quadrant during learning and reverse probe tests for both adult and aged rats. Data represent mean ± SEM for all animals of each group (N = 26–32), **<i>p</i><0.01, ***<i>p</i><0.001.</p>", "links"=>[], "tags"=>["latency"], "article_id"=>365027, "categories"=>["Molecular Biology", "Biological Sciences", "Neuroscience", "Physiology"], "users"=>["Caroline Ménard", "Rémi Quirion"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0028666.g003", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Correlation_between_day_5_latency_and_cognitive_abilities_/365027", "title"=>"Correlation between day 5 latency and cognitive abilities.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-01-06 01:23:47"}
  • {"files"=>["https://ndownloader.figshare.com/files/695049"], "description"=>"<p>A) mGluR1α receptors were immunoprecipitated for PSD and synaptosomes preparations and Homer protein levels were determined by Western Blot. AU showed an increase in Homer 1a/Homer 1 b/c ratio but only in synaptosomes. B) Graphs for average Homer 1 protein levels bound to mGluR1α in the PSD and synaptosomes of each group. C) The aged rats MWM day 5 performances were correlated with the ratio of Homer 1 isoforms and mGluR1α in synaptosomes. D) The ratio of Homer 1 isoform protein levels co-immunoprecipitated with mGluR5 receptors was significantly greater in the PSD of AI rats. Conversely, this ratio was decreased in synaptosomes of AU animals. E) Graphs for average Homer 1 protein levels bound to mGluR5 in the PSD and synaptosomes of each group. F) The Homer 1 isoform ratio co-immunoprecipitated with mGluR5 receptors was strongly correlated with aged rats MWM performances. Data represent mean ± SEM of three separate experiments (N = 4) and are expressed as percentage of control (6-months adult rats), *<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001.</p>", "links"=>[], "tags"=>["homer", "isoforms", "ai", "au", "hippocampal"], "article_id"=>365474, "categories"=>["Molecular Biology", "Biological Sciences", "Neuroscience", "Physiology"], "users"=>["Caroline Ménard", "Rémi Quirion"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0028666.g007", "stats"=>{"downloads"=>1, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparison_of_Homer_1_isoforms_ratio_in_adult_Y_AI_and_AU_hippocampal_formation_/365474", "title"=>"Comparison of Homer 1 isoforms ratio in adult (Y), AI and AU hippocampal formation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-01-06 01:31:14"}
  • {"files"=>["https://ndownloader.figshare.com/files/354483"], "description"=>"<div><p>Normal aging is associated with impairments in cognition, especially learning and memory. However, major individual differences are known to exist. Using the classical Morris Water Maze (MWM) task, we discriminated a population of 24-months old Long Evans aged rats in two groups - memory-impaired (AI) and memory-unimpaired (AU) in comparison with 6-months old adult animals. AI rats presented deficits in learning, reverse memory and retention. At the molecular level, an increase in metabotropic glutamate receptors 5 (mGluR5) was observed in post-synaptic densities (PSD) in the hippocampus of AU rats after training. Scaffolding Homer 1b/c proteins binding to group 1 mGluR facilitate coupling with its signaling effectors while Homer 1a reduces it. Both Homer 1a and 1b/c levels were up-regulated in the hippocampus PSD of AU animals following MWM task. Using immunohistochemistry we further demonstrated that mGluR5 as well as Homer 1b/c stainings were enhanced in the CA1 hippocampus sub-field of AU animals. In fact mGluR5 and Homer 1 isoforms were more abundant and co-localized in the hippocampal dendrites in AU rats. However, the ratio of Homer 1a/Homer 1b/c bound to mGluR5 in the PSD was four times lower for AU animals compared to AI rats. Consequently, AU animals presented higher PKCγ, ERK, p70S6K, mTOR and CREB activation. Finally the expression of immediate early gene Arc/Arg3.1 was shown to be higher in AU rats in accordance with its role in spatial memory consolidation. On the basis of these results, a model of successful cognitive aging with a critical role for mGluR5, Homer 1 proteins and downstream signalling pathways is proposed here.</p> </div>", "links"=>[], "tags"=>["aging", "mglur5", "glutamate", "homer", "proteins", "downstream", "signaling", "pathways"], "article_id"=>130063, "categories"=>["Molecular Biology", "Biological Sciences", "Neuroscience", "Physiology"], "users"=>["Caroline Ménard", "Rémi Quirion"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0028666", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Successful_Cognitive_Aging_in_Rats_A_Role_for_mGluR5_Glutamate_Receptors_Homer_1_Proteins_and_Downstream_Signaling_Pathways/130063", "title"=>"Successful Cognitive Aging in Rats: A Role for mGluR5 Glutamate Receptors, Homer 1 Proteins and Downstream Signaling Pathways", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-01-06 00:01:03"}
  • {"files"=>["https://ndownloader.figshare.com/files/694798"], "description"=>"<p>A) Homer 3 protein level was increased in AU homogenates, while Homer 2 level was increased in AI hippocampal formation. B) Homer 1a and 1 b/c protein levels were considerably increased in AU PSD. The AI animals displayed reduced levels of Homer 3 in the PSD. C) All these alterations in the PSD were correlated to MWM day 5 latencies. D) Similar to mGluR5, Homer 1 b/c immunostaining was reduced in the AI hippocampus, mainly in the CA1 area. Western Blot values represent mean ± SEM of three separate experiments (N = 7) and the data are expressed as percentage of control (6-months adult rats), *<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001.</p>", "links"=>[], "tags"=>["homer", "levels", "ai", "au", "hippocampal"], "article_id"=>365230, "categories"=>["Molecular Biology", "Biological Sciences", "Neuroscience", "Physiology"], "users"=>["Caroline Ménard", "Rémi Quirion"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0028666.g005", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparison_of_Homer_protein_levels_in_adult_Y_AI_and_AU_hippocampal_formations_/365230", "title"=>"Comparison of Homer protein levels in adult (Y), AI and AU hippocampal formations.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-01-06 01:27:10"}
  • {"files"=>["https://ndownloader.figshare.com/files/695313"], "description"=>"<p>A) Arc/Arg 3.1 level was significantly decreased in AI homogenates while Zif 268 was unaltered. B) Arc/Arg 3.1 expression level was correlated to aged rats MWM performance. C) Arc staining was higher for the AU animals in the CA1 sub-field nuclei. Western Blot values represent mean ± SEM of three separate experiments (N = 4) and the data are expressed as percentage of control (6-months adult rats), *<i>p</i><0.05.</p>", "links"=>[], "tags"=>["zif", "268", "levels", "ai", "au", "hippocampal"], "article_id"=>365732, "categories"=>["Molecular Biology", "Biological Sciences", "Neuroscience", "Physiology"], "users"=>["Caroline Ménard", "Rémi Quirion"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0028666.g009", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_IEG_Arc_Arg_3_1_and_Zif_268_levels_in_adult_Y_AI_and_AU_hippocampal_homogenates_/365732", "title"=>"IEG Arc/Arg 3.1 and Zif 268 levels in adult (Y), AI and AU hippocampal homogenates.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-01-06 01:35:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/694464"], "description"=>"<p>A) Behavioral protocol schedule. B) Learning curves of the AI (Impaired), AU (Unimpaired) and 6 months old (Adult) rats for week 1 as well as average probe 1 test results (platform crossings) for all groups. Representative tracking pictures for day 1, day 5 and AI vs AU probe tests are presented. C) Swim speed distribution in all groups during probe 1 test. D) Time spent in target and opposite quadrants during learning of probe test. Data represent mean ± SEM for all animals of each group (N = 26–32), *<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001.</p>", "links"=>[], "tags"=>["aging", "mwm"], "article_id"=>364897, "categories"=>["Molecular Biology", "Biological Sciences", "Neuroscience", "Physiology"], "users"=>["Caroline Ménard", "Rémi Quirion"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0028666.g001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_aging_on_the_MWM_acquisition_task_/364897", "title"=>"Effect of aging on the MWM acquisition task.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-01-06 01:21:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/695173"], "description"=>"<p>A) Representative sketch of the multiple enzymes involved in mGluR5 activation. B) PKCγ Thr674 phosphorylation level is higher in AU homogenates while ERK 1/2 phosphorylation was decreased in AI animals. Total protein levels were unaffected in all groups. C) Thr421/Ser424 sites of p70S6 kinase enzyme were significantly more phosphorylated in AU homogenates. Total protein level remained constant in all groups. D) The strongest variation of phosphorylation level was observed for mTOR (Ser2448) in the AI group. A slight increase of CREB (Ser133) phosphorylation was also detected in AU homogenates. E) PKCγ (Thr674) and mTOR (Ser2448) phosphorylation levels were correlated with the MWM day 5 latencies in aged rats. As shown in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0028666#pone-0028666-g006\" target=\"_blank\">Fig. 6F</a> the expression of PKCγ (Thr674) was higher in dendrites of the CA1 sub-field in AU rats using double immunofluorescence with the neuronal marker MAP2. G) For mTOR (Ser2448), the increase was mostly localized in the CA1 cell bodies for AU rats. Western Blot results are represented as mean ± SEM of three separate experiments (N = 4) and the data are expressed as percentage of control (6-months adult rats), *<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001.</p>", "links"=>[], "tags"=>["signaling", "pathways", "mglur5", "ai", "au"], "article_id"=>365599, "categories"=>["Molecular Biology", "Biological Sciences", "Neuroscience", "Physiology"], "users"=>["Caroline Ménard", "Rémi Quirion"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0028666.g008", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Activation_of_signaling_pathways_related_to_mGluR5_in_adult_Y_AI_and_AU_animals_/365599", "title"=>"Activation of signaling pathways related to mGluR5 in adult (Y), AI and AU animals.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-01-06 01:33:19"}

PMC Usage Stats | Further Information

  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"70", "unique-ip"=>"73", "pdf"=>"53", "year"=>"2012", "figure"=>"35", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"76", "year"=>"2012", "pdf"=>"56", "unique-ip"=>"74", "figure"=>"30", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"62", "unique-ip"=>"66", "pdf"=>"62", "year"=>"2012", "figure"=>"31", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"55", "year"=>"2012", "pdf"=>"51", "unique-ip"=>"45", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"40", "unique-ip"=>"43", "pdf"=>"31", "year"=>"2012", "figure"=>"14", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"83", "year"=>"2012", "pdf"=>"26", "unique-ip"=>"50", "figure"=>"34", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"unique-ip"=>"38", "full-text"=>"40", "pdf"=>"21", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"40", "full-text"=>"42", "pdf"=>"31", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"35", "full-text"=>"36", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"31", "full-text"=>"31", "pdf"=>"19", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"14", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"35", "full-text"=>"37", "pdf"=>"22", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"18", "full-text"=>"20", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"24", "full-text"=>"28", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"23", "full-text"=>"25", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"29", "full-text"=>"29", "pdf"=>"20", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"28", "full-text"=>"23", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"11", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"19", "full-text"=>"21", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"14", "full-text"=>"20", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"25", "full-text"=>"30", "pdf"=>"18", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"35", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"9", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"17", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"17", "full-text"=>"15", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"21", "full-text"=>"12", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"13", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"14", "full-text"=>"17", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"5", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"19", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"18", "full-text"=>"14", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"22", "full-text"=>"14", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"18", "full-text"=>"15", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"13", "full-text"=>"17", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"24", "full-text"=>"26", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[322, 550, 671, 773, 864, 955, 1048, 1135, 1223, 1308, 1387, 1465, 1534, 1602, 1673, 1744, 1813, 1885, 1955, 2026, 2093, 2160, 2228, 2290, 2349]}, {"subject_area"=>"/Biology and life sciences/Anatomy and physiology", "average_usage"=>[307, 540, 660, 756, 847, 936, 1025, 1111, 1199, 1282, 1361, 1432]}, {"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[316, 541, 663, 766, 856, 950, 1041, 1128, 1218, 1302, 1382, 1456, 1526, 1593, 1657, 1729, 1796, 1862, 1930, 1999, 2065, 2132, 2202, 2261, 2319]}, {"subject_area"=>"/Biology and life sciences/Neuroscience", "average_usage"=>[325, 542, 658, 757, 836, 925, 1015, 1095, 1177, 1263, 1338, 1411, 1477, 1537, 1599, 1663, 1726, 1795, 1857, 1921, 1979, 2041, 2099, 2150, 2205]}, {"subject_area"=>"/Biology and life sciences/Psychology", "average_usage"=>[328, 561, 679, 772, 852, 944, 1035, 1122, 1197, 1277, 1354, 1416, 1479, 1549, 1615, 1679, 1740, 1807, 1870, 1937, 1996, 2059, 2118, 2179, 2244]}, {"subject_area"=>"/Biology and life sciences/Zoology", "average_usage"=>[360, 554, 661, 756, 829, 913, 992, 1072, 1144, 1228, 1306, 1378, 1439, 1500, 1558, 1627, 1689, 1754, 1826, 1892, 1951, 2009, 2067, 2130, 2200]}, {"subject_area"=>"/Social sciences/Psychology", "average_usage"=>[336, 562, 679, 770, 848, 938, 1036, 1125, 1198, 1287, 1364, 1425, 1488, 1556, 1618, 1687, 1756, 1817, 1879, 1943, 2011, 2065, 2118, 2186, 2243]}]}
Loading … Spinner
There are currently no alerts