Small Protein-Mediated Quorum Sensing in a Gram-Negative Bacterium
Publication Date
December 12, 2011
Journal
PLOS ONE
Authors
Sang Wook Han, Malinee Sriariyanun, Sang Won Lee, Manoj Sharma, et al
Volume
6
Issue
12
Pages
e29192
DOI
https://dx.plos.org/10.1371/journal.pone.0029192
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0029192
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/22174954
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236232
Europe PMC
http://europepmc.org/abstract/MED/22174954
Web of Science
000298366600061
Scopus
83155167625
Mendeley
http://www.mendeley.com/research/small-proteinmediated-quorum-sensing-gramnegative-bacterium
Events
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Mendeley | Further Information

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Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/704092"], "description"=>"<p>Expression of the <i>raxST, raxB,</i> and <i>raxR</i> genes in PXO99 (10<sup>6</sup> and 10<sup>8</sup> CFU/ml, grey), PXO99<i>Δax21</i> (10<sup>8</sup> CFU/ml, black), and PXO99<i>Δax21</i> (10<sup>8</sup> CFU/ml) supplemented with exogenous addition of the flow-through (FT), 150 mM (150), or 250 mM (250) imidazole buffer-eluted fractions (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029192#pone.0029192.s002\" target=\"_blank\">figure S2</a>) was assessed. Levels of gene expression were calculated relative to 16sRNA expression. Primers specific to each gene are as listed in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029192#pone.0029192.s021\" target=\"_blank\">table S8</a>. Data are mean of four replicates ± standard deviation (SD.).</p>", "links"=>[], "tags"=>["recombinant", "ax21", "complements", "density-dependent"], "article_id"=>374437, "categories"=>["Microbiology", "Immunology", "Plant Biology"], "users"=>["Sang-Wook Han", "Malinee Sriariyanun", "Sang-Won Lee", "Manoj Sharma", "Ofir Bahar", "Zachary Bower", "Pamela C. Ronald"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0029192.g001", "stats"=>{"downloads"=>3, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Purified_recombinant_Ax21_complements_density_dependent_expression_of_raxST_raxB_and_raxR_in_PXO99_916_ax21_/374437", "title"=>"Purified recombinant Ax21 complements density-dependent expression of <i>raxST, raxB</i>, and <i>raxR</i> in PXO99<i>Δax21.</i>", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-12-12 01:13:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/704180"], "description"=>"<p>A heat map represents the ratio of gene expression levels in a log<sub>2</sub>-based pseudocolor scale (red, positive; green, negative). (A) Expression pattern of genes encoding proteins predicted to regulate c-di-GMP turnover in PXO99 (Left) and PXO99<i>Δax21</i> (Right). (B) Ax21 up-regulated genes that are highly expressed in early log phase including bacterial motility genes and xanthan gum biosynthesis. The functions of the genes marked by a star were assessed for phenotypes in <i>Xoo</i> knockout strains (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029192#pone.0029192.s013\" target=\"_blank\">Figure S13</a>).</p>", "links"=>[], "tags"=>["genome", "transcriptomics", "pxo99", "densities", "identifies", "ax21-regulated"], "article_id"=>374526, "categories"=>["Microbiology", "Immunology", "Plant Biology"], "users"=>["Sang-Wook Han", "Malinee Sriariyanun", "Sang-Won Lee", "Manoj Sharma", "Ofir Bahar", "Zachary Bower", "Pamela C. Ronald"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0029192.g002", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Whole_genome_transcriptomics_of_PXO99_vs_PXO99_916_ax21_at_different_population_densities_identifies_Ax21_regulated_genes_/374526", "title"=>"Whole genome transcriptomics of PXO99 vs. PXO99<i>Δax21</i> at different population densities identifies Ax21-regulated genes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-12-12 01:15:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/358515", "https://ndownloader.figshare.com/files/358548", "https://ndownloader.figshare.com/files/358627", "https://ndownloader.figshare.com/files/358685", "https://ndownloader.figshare.com/files/358725", "https://ndownloader.figshare.com/files/358773", "https://ndownloader.figshare.com/files/358834", "https://ndownloader.figshare.com/files/358874", "https://ndownloader.figshare.com/files/358934", "https://ndownloader.figshare.com/files/359004", "https://ndownloader.figshare.com/files/359079", "https://ndownloader.figshare.com/files/359154", "https://ndownloader.figshare.com/files/359197", "https://ndownloader.figshare.com/files/359330", "https://ndownloader.figshare.com/files/359385", "https://ndownloader.figshare.com/files/359433", "https://ndownloader.figshare.com/files/359498", "https://ndownloader.figshare.com/files/359601", "https://ndownloader.figshare.com/files/359651", "https://ndownloader.figshare.com/files/359755", "https://ndownloader.figshare.com/files/359835", "https://ndownloader.figshare.com/files/359882", "https://ndownloader.figshare.com/files/359930", "https://ndownloader.figshare.com/files/359965"], "description"=>"<div><p>The rice XA21 pattern recognition receptor binds a type I secreted sulfated peptide, called axY<sup>S</sup>22, derived from the Ax21 (<u>a</u>ctivator of <u>X</u>A<u>21</u>-mediated immunity) protein. The conservation of Ax21 in all sequenced <em>Xanthomonas spp.</em> and closely related genera suggests that Ax21 serves a key biological function. Here we show that the predicted N-terminal sequence of Ax21 is cleaved prior to secretion outside the cell and that mature Ax21 serves as a quorum sensing (QS) factor in <em>Xanthomonas oryzae</em> pv. <em>oryzae</em>. Ax21-mediated QS controls motility, biofilm formation and virulence. We provide genetic evidence that the <em>Xoo</em> RaxH histidine kinase serves as the bacterial receptor for Ax21. This work establishes a critical role for small protein-mediated QS in a Gram-negative bacterium.</p> </div>", "links"=>[], "tags"=>["protein-mediated", "quorum", "sensing", "gram-negative", "bacterium"], "article_id"=>130875, "categories"=>["Microbiology", "Immunology", "Cell Biology"], "users"=>["Sang-Wook Han", "Malinee Sriariyanun", "Sang-Won Lee", "Manoj Sharma", "Ofir Bahar", "Zachary Bower", "Pamela C. Ronald"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0029192.s001", "https://dx.doi.org/10.1371/journal.pone.0029192.s002", "https://dx.doi.org/10.1371/journal.pone.0029192.s003", "https://dx.doi.org/10.1371/journal.pone.0029192.s004", "https://dx.doi.org/10.1371/journal.pone.0029192.s005", "https://dx.doi.org/10.1371/journal.pone.0029192.s006", "https://dx.doi.org/10.1371/journal.pone.0029192.s007", "https://dx.doi.org/10.1371/journal.pone.0029192.s008", "https://dx.doi.org/10.1371/journal.pone.0029192.s009", "https://dx.doi.org/10.1371/journal.pone.0029192.s010", "https://dx.doi.org/10.1371/journal.pone.0029192.s011", "https://dx.doi.org/10.1371/journal.pone.0029192.s012", "https://dx.doi.org/10.1371/journal.pone.0029192.s013", "https://dx.doi.org/10.1371/journal.pone.0029192.s014", "https://dx.doi.org/10.1371/journal.pone.0029192.s015", "https://dx.doi.org/10.1371/journal.pone.0029192.s016", "https://dx.doi.org/10.1371/journal.pone.0029192.s017", "https://dx.doi.org/10.1371/journal.pone.0029192.s018", "https://dx.doi.org/10.1371/journal.pone.0029192.s019", "https://dx.doi.org/10.1371/journal.pone.0029192.s020", "https://dx.doi.org/10.1371/journal.pone.0029192.s021", "https://dx.doi.org/10.1371/journal.pone.0029192.s022", "https://dx.doi.org/10.1371/journal.pone.0029192.s023", "https://dx.doi.org/10.1371/journal.pone.0029192.s024"], "stats"=>{"downloads"=>7, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Small_Protein_Mediated_Quorum_Sensing_in_a_Gram_Negative_Bacterium/130875", "title"=>"Small Protein-Mediated Quorum Sensing in a Gram-Negative Bacterium", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2011-12-12 00:14:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/704273"], "description"=>"<p>(A) Biofilm formation of PXO99, PXO99<i>Δax21</i>, PXO99<i>Δax21</i> supplemented with exogenous addition of the flow-through (FT), 150 mM (150), or 250 mM (250) imidazole buffer-eluted fractions (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029192#pone.0029192.s002\" target=\"_blank\">figure S2</a>) were measured according to absorbance at A<sub>590</sub> using the polyvinyl chloride plate assay and normalized with the value of PXO99. Bars represent the mean of ten biological replicates ± SD. (B) Aggregation assays were performed using PXO99 and PXO99Δ<i>ax21</i> strains carrying a green fluorescent protein. <i>Xoo</i> strains were observed with a microscope equipped with a fluorescein isothiocyanate filter (excitation filter, 450 to 490 nm; emission filter, 520 nm; dichroic mirror, 510 nm). The bars indicate 10 µm. (C) Swimming motility of PXO99 and PXO99<i>Δax21</i> strains were quantified by measuring the diameter of colony expansion in swimming motility assays. Bars represent the mean of four biological replicates ± SD. (D) Virulence of PXO99 and PXO99<i>Δax21</i> was monitored at low population densities. Rice leaves (TP309 cultivar) were inoculated with PXO99, PXO99Δ<i>raxST</i>, and PXO99Δ<i>ax21</i> strains using the soaking method (10<sup>3</sup> CFU/ml) for two days. Bacterial populations were determined two days after inoculation. Bars represent the mean from at least seven leaves ± SD. The experiment was repeated four times with similar results.</p>", "links"=>[], "tags"=>["regulates", "biofilm"], "article_id"=>374630, "categories"=>["Microbiology", "Immunology", "Plant Biology"], "users"=>["Sang-Wook Han", "Malinee Sriariyanun", "Sang-Won Lee", "Manoj Sharma", "Ofir Bahar", "Zachary Bower", "Pamela C. Ronald"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0029192.g003", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Ax21_regulates_Xoo_biofilm_formation_cell_aggregation_motility_and_virulence_/374630", "title"=>"Ax21 regulates <i>Xoo</i> biofilm formation, cell aggregation, motility, and virulence.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-12-12 01:17:10"}

PMC Usage Stats | Further Information

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Relative Metric

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