Transgenic Potatoes for Potato Cyst Nematode Control Can Replace Pesticide Use without Impact on Soil Quality
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Transgenic potatoes for potato cyst nematode control can replace pesticide use without impact on soil quality", "type"=>"journal", "authors"=>[{"first_name"=>"Jayne", "last_name"=>"Green", "scopus_author_id"=>"7404571326"}, {"first_name"=>"Dong", "last_name"=>"Wang", "scopus_author_id"=>"55628532964"}, {"first_name"=>"Catherine J.", "last_name"=>"Lilley", "scopus_author_id"=>"7004689327"}, {"first_name"=>"Peter E.", "last_name"=>"Urwin", "scopus_author_id"=>"7004487861"}, {"first_name"=>"Howard J.", "last_name"=>"Atkinson", "scopus_author_id"=>"7101883677"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84863181602", "doi"=>"10.1371/journal.pone.0030973", "pui"=>"364271740", "issn"=>"19326203", "pmid"=>"22359559", "isbn"=>"1932-6203", "scopus"=>"2-s2.0-84863181602"}, "id"=>"8ff8409f-6452-377a-b5f5-273120cf5ccc", "abstract"=>"Current and future global crop yields depend upon soil quality to which soil organisms make an important contribution. The European Union seeks to protect European soils and their biodiversity for instance by amending its Directive on pesticide usage. This poses a challenge for control of Globodera pallida (a potato cyst nematode) for which both natural resistance and rotational control are inadequate. One approach of high potential is transgenically based resistance. This work demonstrates the potential in the field of a new transgenic trait for control of G. pallida that suppresses root invasion. It also investigates its impact and that of a second transgenic trait on the non-target soil nematode community. We establish that a peptide that disrupts chemoreception of nematodes without a lethal effect provides resistance to G. pallida in both a containment and a field trial when precisely targeted under control of a root tip-specific promoter. In addition we combine DNA barcoding and quantitative PCR to recognise nematode genera from soil samples without microscope-based observation and use the method for nematode faunal analysis. This approach establishes that the peptide and a cysteine proteinase inhibitor that offer distinct bases for transgenic plant resistance to G. pallida do so without impact on the non-target nematode soil community.", "link"=>"http://www.mendeley.com/research/transgenic-potatoes-potato-cyst-nematode-control-replace-pesticide-without-impact-soil-quality", "reader_count"=>42, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>2, "Student > Doctoral Student"=>1, "Researcher"=>9, "Student > Ph. D. Student"=>8, "Student > Postgraduate"=>1, "Other"=>4, "Student > Master"=>5, "Student > Bachelor"=>8, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>2, "Student > Doctoral Student"=>1, "Researcher"=>9, "Student > Ph. D. Student"=>8, "Student > Postgraduate"=>1, "Other"=>4, "Student > Master"=>5, "Student > Bachelor"=>8, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Environmental Science"=>3, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>33, "Medicine and Dentistry"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>33}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>3}, "Environmental Science"=>{"Environmental Science"=>3}}, "reader_count_by_country"=>{"Serbia and Montenegro"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84863181602"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84863181602?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84863181602&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84863181602&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84863181602", "dc:identifier"=>"SCOPUS_ID:84863181602", "eid"=>"2-s2.0-84863181602", "dc:title"=>"Transgenic potatoes for potato cyst nematode control can replace pesticide use without impact on soil quality", "dc:creator"=>"Green J.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"7", "prism:issueIdentifier"=>"2", "prism:pageRange"=>nil, "prism:coverDate"=>"2012-02-16", "prism:coverDisplayDate"=>"16 February 2012", "prism:doi"=>"10.1371/journal.pone.0030973", "citedby-count"=>"17", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Advanced Technologies (Cambridge) Limited", "affiliation-city"=>"Cambridge", "affiliation-country"=>"United Kingdom"}, {"@_fa"=>"true", "affilname"=>"University of Leeds", "affiliation-city"=>"Leeds", "affiliation-country"=>"United Kingdom"}], "pubmed-id"=>"22359559", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e30973", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0030973", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"65", "xml_views"=>"8", "html_views"=>"206"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"42", "xml_views"=>"8", "html_views"=>"120"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"40", "xml_views"=>"17", "html_views"=>"96"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"49", "xml_views"=>"3", "html_views"=>"128"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"89"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"15", "xml_views"=>"4", "html_views"=>"80"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"27", "xml_views"=>"2", "html_views"=>"93"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"38", "xml_views"=>"1", "html_views"=>"131"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"43", "xml_views"=>"2", "html_views"=>"132"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"83"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"32", "xml_views"=>"0", "html_views"=>"147"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"105"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"121"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"26", "xml_views"=>"3", "html_views"=>"168"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"142"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"126"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"69"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"74"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"75"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"120"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"12", "xml_views"=>"2", "html_views"=>"122"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"89"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"66"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"18", "xml_views"=>"3", "html_views"=>"120"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"18", "xml_views"=>"1", "html_views"=>"111"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"79"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"63"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"79"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"62"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"92"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"2", "html_views"=>"80"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"3", "html_views"=>"69"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"2", "html_views"=>"72"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"65"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"49"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"77"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"28", "xml_views"=>"0", "html_views"=>"110"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"28", "xml_views"=>"1", "html_views"=>"84"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"70"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"93"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"68"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"81"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"92"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"33"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"62"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"38"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"3", "html_views"=>"8"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"8"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"7"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"4"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/680897"], "description"=>"<p>(A) The chemically synthesised peptide inhibited alkaline phosphatase in concentration dependent manner. (B) Sufficient peptide could be collected from root exudates of transgenic potatoes to inhibit the enzyme. Values are means ± sem.</p>", "links"=>[], "tags"=>["alkaline", "phosphatase", "behavioural", "disrupting"], "article_id"=>351379, "categories"=>["Biotechnology", "Plant Biology"], "users"=>["Jayne Green", "Dong Wang", "Catherine J. Lilley", "Peter E. Urwin", "Howard J. Atkinson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0030973.g001", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Inhibition_of_alkaline_phosphatase_by_the_behavioural_disrupting_peptide_/351379", "title"=>"Inhibition of alkaline phosphatase by the behavioural disrupting peptide.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-02-16 00:22:59"}
  • {"files"=>["https://ndownloader.figshare.com/files/681374"], "description"=>"<p>The percentage of each nematode genus present was estimated from three field soil samples using in each case one sub-sample for morphological identification and 3–7 sub-samples for qPCR-based analysis. The functional guild and their position on the coloniser-persister scale (1–5, shown as a suffix) are as previously assigned to each genus <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030973#pone.0030973-Bongers1\" target=\"_blank\">[22]</a>, <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030973#pone.0030973-Ferris1\" target=\"_blank\">[23]</a>. The percentages are based on the number of nematodes observed (morphological analysis) or on all nematodes (>200) extracted from each 100 g sub-sample used to prepare template for qPCR. <i>Anaplectus</i>, <i>Ceratoplectus</i> and <i>Rhabditella</i> were not detected by either morphology or qPCR in these samples. Values for qPCR represent means ± sem.</p>", "links"=>[], "tags"=>["nematode", "genera", "morphology", "qpcr"], "article_id"=>351866, "categories"=>["Biotechnology", "Plant Biology"], "users"=>["Jayne Green", "Dong Wang", "Catherine J. Lilley", "Peter E. Urwin", "Howard J. Atkinson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0030973.t003", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Proportions_of_nematode_genera_determined_by_both_morphology_and_qPCR_for_field_soil_samples_/351866", "title"=>"Proportions of nematode genera determined by both morphology and qPCR for field soil samples.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-02-16 00:31:06"}
  • {"files"=>["https://ndownloader.figshare.com/files/681424"], "description"=>"<p>The functional guilds of each genus <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030973#pone.0030973-Bongers1\" target=\"_blank\">[22]</a> and their standard weightings were used to calculate the components of the food web from which the enrichment (EI) and structural (SI) indices were calculated for each soil sample (see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030973#pone.0030973-Ferris1\" target=\"_blank\">[23]</a> for further details). Values derived from qPCR data represent means ± sem.</p>", "links"=>[], "tags"=>["enrichment", "indices", "nematode", "communities", "morphology"], "article_id"=>351911, "categories"=>["Biotechnology", "Plant Biology"], "users"=>["Jayne Green", "Dong Wang", "Catherine J. Lilley", "Peter E. Urwin", "Howard J. Atkinson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0030973.t004", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Values_for_enrichment_EI_and_structural_SI_indices_of_soil_nematode_communities_determined_by_both_morphology_and_qPCR_/351911", "title"=>"Values for enrichment (EI) and structural (SI) indices of soil nematode communities determined by both morphology and qPCR.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-02-16 00:31:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/681304"], "description"=>"<p>*Ba, bacterivore; Fu, fungivore; Ca, carnivore; Om, omnivore.</p><p>These genera contributed >90% of individual non-parasitic nematodes at the site. The functional guild and their position on the coloniser-persister scale (1–5, shown as a suffix) are as previously assigned to each genus <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030973#pone.0030973-Bongers1\" target=\"_blank\">[22]</a>, <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030973#pone.0030973-Ferris1\" target=\"_blank\">[23]</a>. The sequence identity of the amplified region of 18S small subunit ribosomal RNA gene to that of the most similar genus in the GenBank data set is indicated together with the Accession Number of the most similar database sequence.</p>", "links"=>[], "tags"=>["nematode", "genera", "recovered"], "article_id"=>351797, "categories"=>["Biotechnology", "Plant Biology"], "users"=>["Jayne Green", "Dong Wang", "Catherine J. Lilley", "Peter E. Urwin", "Howard J. Atkinson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0030973.t001", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Free_living_soil_nematode_genera_recovered_at_the_field_site_/351797", "title"=>"Free-living soil nematode genera recovered at the field site.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-02-16 00:29:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/681348"], "description"=>"<p>*F, forward primer; R, reverse primer.</p>", "links"=>[], "tags"=>["pairs", "qpcr", "nematode"], "article_id"=>351837, "categories"=>["Biotechnology", "Plant Biology"], "users"=>["Jayne Green", "Dong Wang", "Catherine J. Lilley", "Peter E. Urwin", "Howard J. Atkinson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0030973.t002", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Primer_pairs_used_in_qPCR_to_identify_each_nematode_genus_/351837", "title"=>"Primer pairs used in qPCR to identify each nematode genus.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-02-16 00:30:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/681151"], "description"=>"<p>The template for each pair of primers was DNA extracted from two hundred nematodes of mixed genera as recovered from the field site. The primers, detailed in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030973#pone-0030973-t002\" target=\"_blank\">Table 2</a> for non-parasitic genera, were designed to amplify SSU sequence from (1) <i>Acrobeloides/Cephalobus</i> (2) <i>Anatonchus</i>, (3) <i>Aphelenchoides</i>, (4) <i>Aporcelaimellus</i>, (5) <i>Coslenchus</i>, (6) <i>Ditylenchus</i>, (7) <i>Eucephalobus</i>, (8) <i>Filenchus</i>, (9) <i>Globodera</i>, (10) <i>Helicotylenchus</i>, (11) <i>Mesodorylaimus</i>, (12) <i>Pellioditis</i>, (13) <i>Pelodera</i>, (14) <i>Rhabditella</i>, (15) <i>Scutylenchus</i>, (16) <i>Ceratoplectus</i> (17) <i>Anaplectus</i>. (M) Hyperladder I marker.</p>", "links"=>[], "tags"=>["products", "amplified", "genus"], "article_id"=>351644, "categories"=>["Biotechnology", "Plant Biology"], "users"=>["Jayne Green", "Dong Wang", "Catherine J. Lilley", "Peter E. Urwin", "Howard J. Atkinson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0030973.g003", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_PCR_products_amplified_using_genus_specific_primers_/351644", "title"=>"PCR products amplified using genus specific primers.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-02-16 00:27:24"}
  • {"files"=>["https://ndownloader.figshare.com/files/347223"], "description"=>"<div><p>Current and future global crop yields depend upon soil quality to which soil organisms make an important contribution. The European Union seeks to protect European soils and their biodiversity for instance by amending its Directive on pesticide usage. This poses a challenge for control of <em>Globodera pallida</em> (a potato cyst nematode) for which both natural resistance and rotational control are inadequate. One approach of high potential is transgenically based resistance. This work demonstrates the potential in the field of a new transgenic trait for control of <em>G. pallida</em> that suppresses root invasion. It also investigates its impact and that of a second transgenic trait on the non-target soil nematode community. We establish that a peptide that disrupts chemoreception of nematodes without a lethal effect provides resistance to <em>G. pallida</em> in both a containment and a field trial when precisely targeted under control of a root tip-specific promoter. In addition we combine DNA barcoding and quantitative PCR to recognise nematode genera from soil samples without microscope-based observation and use the method for nematode faunal analysis. This approach establishes that the peptide and a cysteine proteinase inhibitor that offer distinct bases for transgenic plant resistance to <em>G. pallida</em> do so without impact on the non-target nematode soil community.</p> </div>", "links"=>[], "tags"=>["transgenic", "potatoes", "cyst", "nematode", "pesticide"], "article_id"=>128659, "categories"=>["Biotechnology", "Cell Biology"], "users"=>["Jayne Green", "Dong Wang", "Catherine J. Lilley", "Peter E. Urwin", "Howard J. Atkinson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0030973", "stats"=>{"downloads"=>7, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Transgenic_Potatoes_for_Potato_Cyst_Nematode_Control_Can_Replace_Pesticide_Use_without_Impact_on_Soil_Quality/128659", "title"=>"Transgenic Potatoes for Potato Cyst Nematode Control Can Replace Pesticide Use without Impact on Soil Quality", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-02-16 02:24:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/681228"], "description"=>"<p>The changes for enrichment (ΔEI) and structural indices (ΔSI) were measured in both containment and the field at potato flowering and immediately before harvest. For each trial, soil was sampled from around the roots of oil seed rape (○), untransformed potato cultivars Santé (<b>Δ</b>) and Désirée (<b>X</b>) plus 2 lines of the latter cultivar expressing a cystatin <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030973#pone.0030973-Urwin2\" target=\"_blank\">[9]</a>, <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030973#pone.0030973-Lilley1\" target=\"_blank\">[10]</a> (▪) and 3 lines expressing the peptide (•). The containment trial involved three pots for each plant type. For the field trial there were nine pooled samples for each of four replicate plots/line or plant type. Values are means ± sem.</p>", "links"=>[], "tags"=>["enrichment", "indices", "nematode", "faunal", "pre-plant"], "article_id"=>351716, "categories"=>["Biotechnology", "Plant Biology"], "users"=>["Jayne Green", "Dong Wang", "Catherine J. Lilley", "Peter E. Urwin", "Howard J. Atkinson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0030973.g004", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Changes_in_enrichment_and_structural_indices_for_soil_nematode_faunal_analysis_relative_to_pre_plant_levels_/351716", "title"=>"Changes in enrichment and structural indices for soil nematode faunal analysis relative to pre-plant levels.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-02-16 00:28:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/681066"], "description"=>"<p>Resistance is conferred by expression of the peptide under control of a root-tip specific promoter (<i>MDK4–20</i>) in trials in both a containment glasshouse (light shading) and the field (darker shading) relative to <i>G. pallida</i> eggs in soil after multiplication on untransformed cv Désirée. Values are means ± sem based on 2 replicate samples for each of ten pots per line in containment and pools of nine samples for each of 4 replicate plots per line in the field. The differences between transgenic lines and the corresponding wildtype control are shown (***, P<0.001; **, P<0.01; *, P<0.05; oneway ANOVA with <i>a priori</i> contrasts).</p>", "links"=>[], "tags"=>["transgenic", "behavioural", "disrupting", "peptide"], "article_id"=>351551, "categories"=>["Biotechnology", "Plant Biology"], "users"=>["Jayne Green", "Dong Wang", "Catherine J. Lilley", "Peter E. Urwin", "Howard J. Atkinson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0030973.g002", "stats"=>{"downloads"=>4, "page_views"=>35, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Resistance_to_Globodera_pallida_provided_by_transgenic_expression_of_a_behavioural_disrupting_peptide_in_potato_/351551", "title"=>"Resistance to <i>Globodera pallida</i> provided by transgenic expression of a behavioural disrupting peptide in potato.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-02-16 00:25:51"}

PMC Usage Stats | Further Information

  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"4", "year"=>"2012", "pdf"=>"4", "unique-ip"=>"6", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"42", "unique-ip"=>"38", "pdf"=>"27", "year"=>"2012", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"24", "year"=>"2012", "pdf"=>"12", "unique-ip"=>"27", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"21", "unique-ip"=>"20", "pdf"=>"9", "year"=>"2012", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"10", "year"=>"2012", "pdf"=>"8", "unique-ip"=>"9", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"15", "full-text"=>"12", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"26", "full-text"=>"28", "pdf"=>"21", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"21", "full-text"=>"22", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"35", "full-text"=>"41", "pdf"=>"12", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"24", "full-text"=>"25", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"17", "full-text"=>"13", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"7", "pdf"=>"6", "abstract"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"16", "full-text"=>"21", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"1", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"15", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"1", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"18", "full-text"=>"17", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"9", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[322, 550, 671, 773, 864, 955, 1048, 1135, 1223, 1308, 1387, 1465, 1534, 1602, 1673, 1744, 1813, 1885, 1955, 2026, 2093, 2160, 2228, 2290, 2349]}, {"subject_area"=>"/Biology and life sciences/Agriculture", "average_usage"=>[323, 536, 657, 751, 847, 939, 1032, 1114, 1201, 1279, 1355, 1431, 1516, 1582, 1656, 1719, 1791, 1848, 1919, 2000, 2068, 2127, 2208, 2281, 2359]}, {"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[324, 551, 679, 782, 879, 971, 1064, 1154, 1245, 1328, 1406, 1476, 1541, 1617, 1683, 1754, 1818, 1893, 1962, 2025, 2092, 2164, 2227, 2294, 2355]}, {"subject_area"=>"/Biology and life sciences/Plant science", "average_usage"=>[329, 543, 667, 773, 865, 963, 1066, 1149, 1233, 1323, 1411, 1500, 1588, 1661, 1732, 1803, 1887, 1978, 2057, 2138, 2203, 2283, 2363, 2419, 2493]}, {"subject_area"=>"/Ecology and environmental sciences", "average_usage"=>[348, 541, 638, 730, 827, 907, 993, 1074, 1152, 1232, 1310, 1379, 1442, 1507, 1576, 1648, 1715, 1786, 1854, 1926, 1991, 2059, 2125, 2181, 2249]}, {"subject_area"=>"/Ecology and environmental sciences/Soil science", "average_usage"=>[245, 418, 522, 617, 706, 782, 859, 945, 1019, 1097, 1172, 1239, 1327, 1409, 1487, 1536, 1610, 1670, 1719, 1787, 1854, 1919, 1981, 2065, 2128]}]}

F1000Prime | Further Information

Loading … Spinner
There are currently no alerts