Multiple Intravenous Administrations of Human Umbilical Cord Blood Cells Benefit in a Mouse Model of ALS
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Multiple intravenous administrations of human umbilical cord blood cells benefit in a mouse model of ALS", "type"=>"journal", "authors"=>[{"first_name"=>"Svitlana", "last_name"=>"Garbuzova-Davis", "scopus_author_id"=>"6602519016"}, {"first_name"=>"Maria C O", "last_name"=>"Rodrigues", "scopus_author_id"=>"25643611000"}, {"first_name"=>"Santhia", "last_name"=>"Mirtyl", "scopus_author_id"=>"54947346900"}, {"first_name"=>"Shanna", "last_name"=>"Turner", "scopus_author_id"=>"54946630100"}, {"first_name"=>"Shazia", "last_name"=>"Mitha", "scopus_author_id"=>"54949043100"}, {"first_name"=>"Jasmine", "last_name"=>"Sodhi", "scopus_author_id"=>"54947364800"}, {"first_name"=>"Subatha", "last_name"=>"Suthakaran", "scopus_author_id"=>"54947266200"}, {"first_name"=>"David J.", "last_name"=>"Eve", "scopus_author_id"=>"18041527800"}, {"first_name"=>"Cyndy D.", "last_name"=>"Sanberg", "scopus_author_id"=>"6507233782"}, {"first_name"=>"Nicole", "last_name"=>"Kuzmin-Nichols", "scopus_author_id"=>"6504042119"}, {"first_name"=>"Paul R.", "last_name"=>"Sanberg", "scopus_author_id"=>"36046821700"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84856557925", "doi"=>"10.1371/journal.pone.0031254", "pui"=>"364189171", "issn"=>"19326203", "pmid"=>"22319620", "scopus"=>"2-s2.0-84856557925"}, "id"=>"a1517746-e968-35c4-a0e8-4838b270a815", "abstract"=>"BACKGROUND: A promising therapeutic strategy for amyotrophic lateral sclerosis (ALS) is the use of cell-based therapies that can protect motor neurons and thereby retard disease progression. We recently showed that a single large dose (25 × 10⁶ cells) of mononuclear cells from human umbilical cord blood (MNC hUCB) administered intravenously to pre-symptomatic G93A SOD1 mice is optimal in delaying disease progression and increasing lifespan. However, this single high cell dose is impractical for clinical use. The aim of the present pre-clinical translation study was therefore to evaluate the effects of multiple low dose systemic injections of MNC hUCB cell into G93A SOD1 mice at different disease stages.\\n\\nMETHODOLOGY/PRINCIPAL FINDINGS: Mice received weekly intravenous injections of MNC hUCB or media. Symptomatic mice received 10⁶ or 2.5 × 10⁶ cells from 13 weeks of age. A third, pre-symptomatic, group received 10⁶ cells from 9 weeks of age. Control groups were media-injected G93A and mice carrying the normal hSOD1 gene. Motor function tests and various assays determined cell effects. Administered cell distribution, motor neuron counts, and glial cell densities were analyzed in mouse spinal cords. Results showed that mice receiving 10⁶ cells pre-symptomatically or 2.5 × 10⁶ cells symptomatically significantly delayed functional deterioration, increased lifespan and had higher motor neuron counts than media mice. Astrocytes and microglia were significantly reduced in all cell-treated groups.\\n\\nCONCLUSIONS/SIGNIFICANCE: These results demonstrate that multiple injections of MNC hUCB cells, even beginning at the symptomatic disease stage, could benefit disease outcomes by protecting motor neurons from inflammatory effectors. This multiple cell infusion approach may promote future clinical studies.", "link"=>"http://www.mendeley.com/research/multiple-intravenous-administrations-human-umbilical-cord-blood-cells-benefit-mouse-model-als", "reader_count"=>34, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>2, "Librarian"=>1, "Student > Doctoral Student"=>1, "Researcher"=>2, "Student > Ph. D. Student"=>8, "Student > Postgraduate"=>2, "Other"=>2, "Student > Master"=>7, "Student > Bachelor"=>6, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>2, "Librarian"=>1, "Student > Doctoral Student"=>1, "Researcher"=>2, "Student > Ph. D. Student"=>8, "Student > Postgraduate"=>2, "Other"=>2, "Student > Master"=>7, "Student > Bachelor"=>6, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>16, "Medicine and Dentistry"=>9, "Psychology"=>2, "Immunology and Microbiology"=>1, "Economics, Econometrics and Finance"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>9}, "Psychology"=>{"Psychology"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Economics, Econometrics and Finance"=>{"Economics, Econometrics and Finance"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>16}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>3}}, "reader_count_by_country"=>{"United Kingdom"=>1, "Australia"=>2, "Germany"=>1}, "group_count"=>2}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84856557925"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84856557925?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84856557925&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84856557925&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84856557925", "dc:identifier"=>"SCOPUS_ID:84856557925", "eid"=>"2-s2.0-84856557925", "dc:title"=>"Multiple intravenous administrations of human umbilical cord blood cells benefit in a mouse model of ALS", "dc:creator"=>"Garbuzova-Davis S.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"7", "prism:issueIdentifier"=>"2", "prism:pageRange"=>nil, "prism:coverDate"=>"2012-02-03", "prism:coverDisplayDate"=>"3 February 2012", "prism:doi"=>"10.1371/journal.pone.0031254", "citedby-count"=>"42", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Morsani College of Medicine", "affiliation-city"=>"Tampa", "affiliation-country"=>"United States"}, {"@_fa"=>"true", "affilname"=>"University of South Florida, Tampa", "affiliation-city"=>"Tampa", "affiliation-country"=>"United States"}], "pubmed-id"=>"22319620", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e31254", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0031254", "share_count"=>31, "like_count"=>5, "comment_count"=>0, "click_count"=>0, "total_count"=>36}

Journal Comments | Further Information

Counter

  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"181", "xml_views"=>"23", "html_views"=>"839"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"28", "xml_views"=>"8", "html_views"=>"368"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"39", "xml_views"=>"17", "html_views"=>"106"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"96"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"69"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"13", "xml_views"=>"5", "html_views"=>"53"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"79"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"103"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"52"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"110"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"49"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"29"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"91"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"98"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"95"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"85"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"65"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"65"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"46"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"17"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"77"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"50"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"27"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"72"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"77"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"66"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"57"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"4", "html_views"=>"2"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"8"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"8"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"4"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"3", "html_views"=>"4"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"10", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"11", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"1"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/686785"], "description"=>"<p>Administered MNC hUCB cells were identified immunohistochemically by a human-specific marker (HuNu) in the spinal cord of cell-treated mice at 17 weeks of age, 4 weeks (symptomatic) or 8 weeks (pre-symptomatic) post-transplant. In the total area of cervical (<b>A</b>) and lumbar (<b>B</b>) cervical spinal cord, HuNu positive MNC hUCB cells were found irrespective (p>0.05) of injected cell doses or time beginning treatment. In all cell-treated mice, more than 50% of the observed cells were in ventral horn gray matter. (<b>C</b>) Immunohistochemical staining of MNC hUCB cells in the lumbar spinal cord. MNC hUCB cells positive for HuNu (green, arrow) were detected in the lumbar spinal cord of mice receiving 2.5×10<sup>6</sup> (<b>a</b>) or 1×10<sup>6</sup> (<b>b</b>) cells symptomatically or 1×10<sup>6</sup> cells pre-symptomatically (<b>c</b>). Cells were frequently observed inside the capillary lumen, but also in the spinal cord parenchyma. (<b>a′</b>), (<b>b′</b>), and (<b>c′</b>) are merged images with DAPI. Scale bar: a–c′ is 50 µm.</p>", "links"=>[], "tags"=>["mnc", "hucb", "cells", "spinal", "g93a"], "article_id"=>357260, "categories"=>["Neuroscience", "Immunology"], "users"=>["Svitlana Garbuzova-Davis", "Maria C. O. Rodrigues", "Santhia Mirtyl", "Shanna Turner", "Shazia Mitha", "Jasmine Sodhi", "Subatha Suthakaran", "David J. Eve", "Cyndy D. Sanberg", "Nicole Kuzmin-Nichols", "Paul R. Sanberg"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0031254.g003", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Distribution_of_MNC_hUCB_cells_in_the_spinal_cord_of_G93A_mice_/357260", "title"=>"Distribution of MNC hUCB cells in the spinal cord of G93A mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 09:24:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/687170"], "description"=>"<p>Microglial densities were measured in the cervical (<b>A</b>) and lumbar (<b>B</b>) ventral horns of G93A mice at 17 weeks of age and at end-stage of disease. Microglial densities were significantly (p<0.001) higher in Media-injected mice (<i>Gr 4</i>) compared to control mice (<i>Gr 5</i>) of the same ages. MNC hUCB cell administrations significantly (p<0.001) decreased the number of microglia in the spinal cord of G93A mice compared to Media mice. No significant differences were detected between the cell-treated groups. *p<0.05, **p<0.01, ***p<0.001. (<b>C</b>) Immunohistochemical staining of microglia in the lumbar spinal cord at 17 weeks of age. Microglial cells stained for anti-Iba-1 antibody were sparse in controls (<b>a</b>) and microgliosis was noted in Media-treated animals (<b>b</b>). MNC hUCB cells decreased microglial density in mice from <i>Gr 1</i> (<b>c</b>), <i>Gr 2</i> (<b>d</b>), and <i>Gr 3</i> (<b>e</b>). Morphological analysis of microglial cells determined numerous activated cells with large cell bodies and short processes in Media-injected mice, whereas ramified microglia were mostly observed in cell-treated animals, particularly in <i>Gr 1</i> and <i>Gr 3</i> mice and controls (inserts in a–e). Scale bar: a–e is 200 µm; in a–e inserts is 25 µm.</p>", "links"=>[], "tags"=>["microglial", "cells", "spinal", "cords", "g93a"], "article_id"=>357641, "categories"=>["Neuroscience", "Immunology"], "users"=>["Svitlana Garbuzova-Davis", "Maria C. O. Rodrigues", "Santhia Mirtyl", "Shanna Turner", "Shazia Mitha", "Jasmine Sodhi", "Subatha Suthakaran", "David J. Eve", "Cyndy D. Sanberg", "Nicole Kuzmin-Nichols", "Paul R. Sanberg"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0031254.g006", "stats"=>{"downloads"=>0, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Characteristics_of_microglial_cells_in_the_spinal_cords_of_G93A_mice_/357641", "title"=>"Characteristics of microglial cells in the spinal cords of G93A mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 09:26:47"}
  • {"files"=>["https://ndownloader.figshare.com/files/686647"], "description"=>"<p>(<b>A</b>) Time elapsed until animals lost 15% of their maximum body weight. Mice receiving 1×10<sup>6</sup> cells pre-symptomatically (<i>Gr 3</i>) significantly () maintained body weight vs. Media (<i>Gr 4</i>) mice. A similar trend was observed in mice treated with 2.5×10<sup>6</sup> cells (<i>Gr 1</i>) beginning at symptomatic disease stage. (<b>B</b>) Time elapsed until hindlimb extension scores deteriorated by 70% of the initial score. The <i>Gr 1</i> and <i>Gr 3</i> mice significantly () delayed decline of hindlimb extension compared to <i>Gr 4</i> mice. A significant difference was also detected between <i>Gr 3</i> and <i>Gr 2</i> mice receiving 1×10<sup>6</sup> cells at pre-symptomatic or symptomatic stage of disease, respectively. (<b>C</b>) Time elapsed until muscle strength decreased by 70% from the maximum value. Mice from <i>Gr 3</i> significantly () delayed muscle strength losses vs. <i>Gr 4</i>. <i>Gr 1</i> mice tended to maintain muscle strength post-transplant. (<b>D</b>) Time elapsed until rotarod latency decreased by 70% of the maximum value. Only mice from <i>Gr 3</i> performed better on the rotarod than other cell-treated mice and tended towards significance () taking more time to decrease latency by over 70% of the maximum value compared to <i>Gr 4</i>.</p>", "links"=>[], "tags"=>["progression", "g93a", "mice", "kaplan-meier"], "article_id"=>357126, "categories"=>["Neuroscience", "Immunology"], "users"=>["Svitlana Garbuzova-Davis", "Maria C. O. Rodrigues", "Santhia Mirtyl", "Shanna Turner", "Shazia Mitha", "Jasmine Sodhi", "Subatha Suthakaran", "David J. Eve", "Cyndy D. Sanberg", "Nicole Kuzmin-Nichols", "Paul R. Sanberg"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0031254.g002", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Evaluations_of_disease_progression_in_G93A_mice_through_Kaplan_Meier_analysis_/357126", "title"=>"Evaluations of disease progression in G93A mice through Kaplan-Meier analysis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 09:24:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/687327"], "description"=>"<p>Astrocyte densities were measured in the cervical (<b>A</b>) and lumbar (<b>B</b>) ventral horns of G93A mice at 17 weeks of age and at end-stage of disease. Astrocytic densities were significantly (p<0.001) higher in Media-injected mice (<i>Gr 4</i>) at 17 weeks of age and at end-stage of disease vs. controls (<i>Gr 5</i>) of the same ages. Significant (p<0.001) decrease in the number of astrocytes was determined in cell-treated G93A mice compared to Media mice. No significant statistical differences were detected between the cell-treated groups. Higher number of reactive astrocytes was observed in Media-injected mice at 17 weeks of age and at end-stage disease compared to cell-treated animals. ***p<0.001. (<b>C</b>) Immunohistochemical staining of astrocytes in the lumbar spinal cord of G93A mice at 17 weeks of age. Anti-GFAP antibody staining showed low astrocyte density in controls (<b>a</b>) and astrocytosis in Media-treated animals (<b>b</b>). Considerably decreased actrocytic density was observed in mice from <i>Gr 1</i> (<b>c</b>), <i>Gr 2</i> (<b>d</b>), and <i>Gr 3</i> (<b>e</b>). Astrocyte cell reactivity was also reduced in cell-treated mice vs. Media-injected mice (inserts in a–e). Scale bar: a–e is 200 µm; in a–e inserts is 25 µm.</p>", "links"=>[], "tags"=>["astrocytes", "spinal", "g93a"], "article_id"=>357803, "categories"=>["Neuroscience", "Immunology"], "users"=>["Svitlana Garbuzova-Davis", "Maria C. O. Rodrigues", "Santhia Mirtyl", "Shanna Turner", "Shazia Mitha", "Jasmine Sodhi", "Subatha Suthakaran", "David J. Eve", "Cyndy D. Sanberg", "Nicole Kuzmin-Nichols", "Paul R. Sanberg"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0031254.g007", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Characteristics_of_astrocytes_in_the_spinal_cord_of_G93A_mice_/357803", "title"=>"Characteristics of astrocytes in the spinal cord of G93A mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 09:27:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/686476"], "description"=>"<p>(<b>A</b>) Kaplan-Meier survival curves for G93A mice receiving 2.5×10<sup>6</sup> (<i>Gr 1</i>) or 1×10<sup>6</sup> (<i>Gr 2</i>) cells at symptomatic disease stage and 1×10<sup>6</sup> (<i>Gr 3</i>) cells pre-symptomatically. Control group was Media-injected mice (<i>Gr 4</i>). Significant () increases in survival were determined in mice receiving 1×10<sup>6</sup> cells at pre-symptomatic stage (p = 0.0015) and 2.5×10<sup>6</sup> cells at symptomatic stage (p = 0.0022) vs. the Media-injected group. Survival of the mouse group receiving 1×10<sup>6</sup> cells pre-symptomatically tended towards significance compared to survival of mice receiving same cell dose at symptomatic stage (p = 0.0595). (<b>B</b>) Percentages of surviving mice within age ranges. Media-injected animals survived no longer than 19.5 weeks, whereas 30% of mice receiving 2.5×10<sup>6</sup> (<i>Gr 1</i>, symptomatic) or 1×10<sup>6</sup> cells (<i>Gr 3</i>, pre-symptomatic) and 14.3% mice administered with 1×10<sup>6</sup> cells (<i>Gr 2</i>) at symptomatic stage survived more than 140 days and 10% of mice from <i>Gr 3</i> (1×10<sup>6</sup> cells, pre-symptomatic) were alive for more than 150 days.</p>", "links"=>[], "tags"=>["mnc", "hucb", "administrations", "lifespan", "g93a"], "article_id"=>356939, "categories"=>["Neuroscience", "Immunology"], "users"=>["Svitlana Garbuzova-Davis", "Maria C. O. Rodrigues", "Santhia Mirtyl", "Shanna Turner", "Shazia Mitha", "Jasmine Sodhi", "Subatha Suthakaran", "David J. Eve", "Cyndy D. Sanberg", "Nicole Kuzmin-Nichols", "Paul R. Sanberg"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0031254.g001", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_multiple_MNC_hUCB_cell_administrations_on_lifespan_of_G93A_mice_/356939", "title"=>"Effect of multiple MNC hUCB cell administrations on lifespan of G93A mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 09:23:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/686894"], "description"=>"<p>MNC hUCB cells immunohistochemically positive for HuNu (green, arrows) were detected in the lung, liver, kidney, and spleen of mice receiving 2.5×10<sup>6</sup> (<b>a, d, g, j</b>) or 1×10<sup>6</sup> (<b>b, e, h, k</b>) cells symptomatically or 1×10<sup>6</sup> cells pre-symptomatically (<b>c, f, i, l</b>). In the liver, lung and kidney, few cells were identified. In the spleen, a high density of HuNu cells was determined in all cell-treated mice (<b>j–l</b>). Scale bar: a–i is 50 µm; j–l is 200 µm.</p>", "links"=>[], "tags"=>["mnc", "hucb", "cells", "kidney", "spleen", "g93a"], "article_id"=>357368, "categories"=>["Neuroscience", "Immunology"], "users"=>["Svitlana Garbuzova-Davis", "Maria C. O. Rodrigues", "Santhia Mirtyl", "Shanna Turner", "Shazia Mitha", "Jasmine Sodhi", "Subatha Suthakaran", "David J. Eve", "Cyndy D. Sanberg", "Nicole Kuzmin-Nichols", "Paul R. Sanberg"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0031254.g004", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Distribution_of_MNC_hUCB_cells_in_the_lung_liver_kidney_and_spleen_of_G93A_mice_/357368", "title"=>"Distribution of MNC hUCB cells in the lung, liver, kidney and spleen of G93A mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 09:25:22"}
  • {"files"=>["https://ndownloader.figshare.com/files/687016"], "description"=>"<p>Motor neuron counts were performed in the cervical (<b>A</b>) and lumbar (<b>B</b>) ventral horns of G93A mice at 17 weeks of age and at end-stage of disease. Mice receiving 2.5×10<sup>6</sup> cells symptomatically (<i>Gr 1</i>) or 1×10<sup>6</sup> cells pre-symptomatically (<i>Gr 3</i>) had significantly higher motor neuron densities than the Media group (<i>Gr 4</i>) at 17 weeks of age or at end-stage of disease. In both cervical and lumbar spinal cords, motor neuron densities between <i>Gr 2</i> (1×10<sup>6</sup> cells, symptomatic) and Media-injected (<i>Gr 4</i>) mice showed no significant differences (p>0.05). *p<0.05, **p<0.01, ***p<0.001. (<b>C</b>) Immunohistochemical staining of motor neurons in the lumbar spinal cord of G93A mice at 17 weeks of age. Motor neuron staining for anti-choline acetyltransferase (anti-ChAT) antibody showed healthy motor neurons in controls (<b>a</b>) although only a few neurons survived in the Media-treated animals (<b>b</b>). Cell-treated mice with (<b>c</b>) 2.5×10<sup>6</sup> cells symptomatically (<i>Gr 1</i>) and (<b>e</b>) 1×10<sup>6</sup> cells pre-symptomatically (<i>Gr 3</i>) demonstrated higher motor neuron survival than (<b>d</b>) mice receiving 1×10<sup>6</sup> cells symptomatically (<i>Gr 2</i>). Scale bar: a–e is 50 µm.</p>", "links"=>[], "tags"=>["neuron", "spinal", "g93a"], "article_id"=>357491, "categories"=>["Neuroscience", "Immunology"], "users"=>["Svitlana Garbuzova-Davis", "Maria C. O. Rodrigues", "Santhia Mirtyl", "Shanna Turner", "Shazia Mitha", "Jasmine Sodhi", "Subatha Suthakaran", "David J. Eve", "Cyndy D. Sanberg", "Nicole Kuzmin-Nichols", "Paul R. Sanberg"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0031254.g005", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Characteristics_of_motor_neuron_survival_in_the_spinal_cord_of_G93A_mice_/357491", "title"=>"Characteristics of motor neuron survival in the spinal cord of G93A mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 09:26:01"}

PMC Usage Stats | Further Information

  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"71", "year"=>"2012", "pdf"=>"63", "unique-ip"=>"77", "figure"=>"29", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"40", "unique-ip"=>"41", "pdf"=>"39", "year"=>"2012", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"38", "year"=>"2012", "pdf"=>"12", "unique-ip"=>"36", "figure"=>"13", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"18", "unique-ip"=>"17", "pdf"=>"12", "year"=>"2012", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"13", "year"=>"2012", "pdf"=>"10", "unique-ip"=>"15", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"29", "full-text"=>"28", "pdf"=>"20", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"6", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"23", "full-text"=>"19", "pdf"=>"13", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"19", "full-text"=>"19", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"22", "full-text"=>"22", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"18", "full-text"=>"17", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"10", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"18", "full-text"=>"19", "pdf"=>"6", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"13", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"13", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"1", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"15", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"18", "full-text"=>"16", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"19", "full-text"=>"26", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"11", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"12", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"15", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"15", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"17", "full-text"=>"21", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"20", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"15", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"10"}

Relative Metric

{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[322, 550, 671, 773, 864, 955, 1048, 1135, 1223, 1308, 1387, 1465, 1534, 1602, 1673, 1744, 1813, 1885, 1955, 2026, 2093, 2160, 2228, 2290, 2349]}, {"subject_area"=>"/Biology and life sciences/Neuroscience", "average_usage"=>[325, 542, 658, 757, 836, 925, 1015, 1095, 1177, 1263, 1338, 1411, 1477, 1537, 1599, 1663, 1726, 1795, 1857, 1921, 1979, 2041, 2099, 2150, 2205]}, {"subject_area"=>"/Biology and life sciences/Physiology", "average_usage"=>[307, 536, 653, 753, 839, 926, 1017, 1103, 1189, 1268, 1348, 1425, 1492, 1557, 1620, 1686, 1759, 1825, 1891, 1950, 2014, 2079, 2141, 2200, 2255]}, {"subject_area"=>"/Medicine and health sciences/Hematology", "average_usage"=>[300, 532, 651, 749, 842, 929, 1022, 1106, 1192, 1267, 1351, 1423, 1489, 1553, 1631, 1694, 1764, 1835, 1899, 1964, 2027, 2087, 2159, 2228, 2280]}, {"subject_area"=>"/Medicine and health sciences/Physiology", "average_usage"=>[308, 535, 653, 754, 847, 930, 1020, 1103, 1188, 1268, 1351, 1420, 1487, 1550, 1614, 1679, 1757, 1830, 1898, 1961, 2024, 2086, 2150, 2216, 2266]}]}
Loading … Spinner
There are currently no alerts