Primary Cultures of Glomerular Parietal Epithelial Cells or Podocytes with Proven Origin
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Primary cultures of glomerular parietal epithelial cells or podocytes with proven origin", "type"=>"journal", "authors"=>[{"first_name"=>"Nazanin", "last_name"=>"Kabgani", "scopus_author_id"=>"26423052900"}, {"first_name"=>"Tamara", "last_name"=>"Grigoleit", "scopus_author_id"=>"55193461000"}, {"first_name"=>"Kevin", "last_name"=>"Schulte", "scopus_author_id"=>"55193155700"}, {"first_name"=>"Antonio", "last_name"=>"Sechi", "scopus_author_id"=>"7004565542"}, {"first_name"=>"Sibille", "last_name"=>"Sauer-Lehnen", "scopus_author_id"=>"6507103658"}, {"first_name"=>"Carmen", "last_name"=>"Tag", "scopus_author_id"=>"6505979881"}, {"first_name"=>"Peter", "last_name"=>"Boor", "scopus_author_id"=>"16314805700"}, {"first_name"=>"Christoph", "last_name"=>"Kuppe", "scopus_author_id"=>"36662350000"}, {"first_name"=>"Gregor", "last_name"=>"Warsow", "scopus_author_id"=>"36646935500"}, {"first_name"=>"Sandra", "last_name"=>"Schordan", "scopus_author_id"=>"35100964400"}, {"first_name"=>"Jörg", "last_name"=>"Mostertz", "scopus_author_id"=>"6505824175"}, {"first_name"=>"Ravi Kumar", "last_name"=>"Chilukoti", "scopus_author_id"=>"49863043400"}, {"first_name"=>"Georg", "last_name"=>"Homuth", "scopus_author_id"=>"56357082900"}, {"first_name"=>"Nicole", "last_name"=>"Endlich", "scopus_author_id"=>"6603257929"}, {"first_name"=>"Frank", "last_name"=>"Tacke", "scopus_author_id"=>"6602670880"}, {"first_name"=>"Ralf", "last_name"=>"Weiskirchen", "scopus_author_id"=>"7004155039"}, {"first_name"=>"Georg", "last_name"=>"Fuellen", "scopus_author_id"=>"6603052627"}, {"first_name"=>"Karlhans", "last_name"=>"Endlich", "scopus_author_id"=>"55949229600"}, {"first_name"=>"Jürgen", "last_name"=>"Floege", "scopus_author_id"=>"55961563700"}, {"first_name"=>"Bart", "last_name"=>"Smeets", "scopus_author_id"=>"55623910600"}, {"first_name"=>"Marcus J.", "last_name"=>"Moeller", "scopus_author_id"=>"55621806300"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pmid"=>"22529955", "doi"=>"10.1371/journal.pone.0034907", "pui"=>"364660910", "issn"=>"19326203", "sgr"=>"84859974901", "scopus"=>"2-s2.0-84859974901"}, "id"=>"e777a725-0c6e-361b-9d03-47a827135180", "abstract"=>"Parietal epithelial cells (PECs) are crucially involved in the pathogenesis of rapidly progressive glomerulonephritis (RPGN) as well as in focal and segmental glomerulosclerosis (FSGS). In this study, transgenic mouse lines were used to isolate pure, genetically tagged primary cultures of PECs or podocytes using FACsorting. By this approach, the morphology of primary glomerular epithelial cells in culture could be resolved: Primary podocytes formed either large cells with intracytoplasmatic extensions or smaller spindle shaped cells, depending on specific culture conditions. Primary PECs were small and exhibited a spindle-shaped or polygonal morphology. In the very early phases of primary culture, rapid changes in gene expression (e.g. of WT-1 and Pax-2) were observed. However, after prolonged culture primary PECs and podocytes still segregated clearly in a transcriptome analysis--demonstrating that the origin of primary cell cultures is important. Of the classical markers, synaptopodin and podoplanin expression were differentially regulated the most in primary PEC and podocyte cultures. However, no expression of any endogenous gene allowed to differentiate between the two cell types in culture. Finally, we show that the transcription factor WT1 is also expressed by PECs. In summary, genetic tagging of PECs and podocytes is a novel and necessary tool to derive pure primary cultures with proven origin. These cultures will be a powerful tool for the emerging field of parietal epithelial cell biology.", "link"=>"http://www.mendeley.com/research/primary-cultures-glomerular-parietal-epithelial-cells-podocytes-proven-origin", "reader_count"=>45, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>4, "Student > Doctoral Student"=>4, "Researcher"=>9, "Student > Ph. D. Student"=>9, "Student > Postgraduate"=>4, "Student > Master"=>2, "Other"=>6, "Student > Bachelor"=>2, "Lecturer"=>1, "Professor"=>3}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>4, "Student > Doctoral Student"=>4, "Researcher"=>9, "Student > Ph. D. Student"=>9, "Student > Postgraduate"=>4, "Student > Master"=>2, "Other"=>6, "Student > Bachelor"=>2, "Lecturer"=>1, "Professor"=>3}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>3, "Agricultural and Biological Sciences"=>20, "Medicine and Dentistry"=>18, "Pharmacology, Toxicology and Pharmaceutical Science"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>18}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>20}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Unspecified"=>{"Unspecified"=>2}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>2}}, "reader_count_by_country"=>{"Czech Republic"=>1, "Korea (South)"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84859974901"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84859974901?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84859974901&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84859974901&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84859974901", "dc:identifier"=>"SCOPUS_ID:84859974901", "eid"=>"2-s2.0-84859974901", "dc:title"=>"Primary cultures of glomerular parietal epithelial cells or podocytes with proven origin", "dc:creator"=>"Kabgani N.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"7", "prism:issueIdentifier"=>"4", "prism:pageRange"=>nil, "prism:coverDate"=>"2012-04-18", "prism:coverDisplayDate"=>"18 April 2012", "prism:doi"=>"10.1371/journal.pone.0034907", "citedby-count"=>"41", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Rheinisch-Westfälische Technische Hochschule Aachen", "affiliation-city"=>"Aachen", "affiliation-country"=>"Germany"}], "pubmed-id"=>"22529955", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e34907", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true, "freetoread"=>{"value"=>[{"$"=>"all"}, {"$"=>"publisherfullgold"}, {"$"=>"repository"}, {"$"=>"repositoryvor"}, {"$"=>"repositoryam"}]}, "freetoreadLabel"=>{"value"=>[{"$"=>"All Open Access"}, {"$"=>"Gold"}, {"$"=>"Green"}]}}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0034907", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"78", "xml_views"=>"11", "html_views"=>"277"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"87", "xml_views"=>"5", "html_views"=>"155"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"93"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"31", "xml_views"=>"1", "html_views"=>"59"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"35", "xml_views"=>"5", "html_views"=>"61"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"123"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"30", "xml_views"=>"0", "html_views"=>"102"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"69"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"126"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"118"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"30", "xml_views"=>"0", "html_views"=>"135"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"23", "xml_views"=>"1", "html_views"=>"145"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"28", "xml_views"=>"1", "html_views"=>"82"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"106"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"87"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"88"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"68"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"26", "xml_views"=>"5", "html_views"=>"102"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"22", "xml_views"=>"1", "html_views"=>"179"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"181"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"132"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"84"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"24", "xml_views"=>"3", "html_views"=>"133"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"2", "html_views"=>"132"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"123"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"111"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"135"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"15", "xml_views"=>"2", "html_views"=>"109"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"146"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"152"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"132"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"21", "xml_views"=>"2", "html_views"=>"145"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"113"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"84"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"88"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"50"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"18", "xml_views"=>"1", "html_views"=>"57"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"77"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"78"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"2", "html_views"=>"70"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"86"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"26", "xml_views"=>"1", "html_views"=>"84"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"84"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"95"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"80"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"63"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"22", "xml_views"=>"1", "html_views"=>"76"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"82"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"90"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"2", "html_views"=>"60"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"52"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"3", "html_views"=>"25"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"2", "html_views"=>"45"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"36"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"6", "html_views"=>"48"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"2", "html_views"=>"24"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"20", "xml_views"=>"3", "html_views"=>"18"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"15", "xml_views"=>"2", "html_views"=>"19"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"25", "xml_views"=>"2", "html_views"=>"17"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"10", "year"=>"2020", "pdf_views"=>"22", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"11", "year"=>"2020", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"43"}
  • {"month"=>"12", "year"=>"2020", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"1", "year"=>"2021", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"8"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/652419"], "description"=>"<p>A. First capsulated or decapsulated glomeruli were purified using magnetic beads (arrow with tails, >90% purity), to prepare primary mixed podocyte or parietal cell cultures respectively. For direct comparison, a capsulated glomerulus (arrows) is shown next to a decapsulated glomerulus (arrowheads). B, C. After 7–14 days of culture in EGM-MV media, primary outgrowths were obtained and stained for the genetic marker (X-gal). D, E. Next, suspensions of primary cells were treated with a luminescent substrate for beta-galactosidase (“labeled”, D′, E′) or not (controls, D, E) and were FACS-sorted using the indicated gate (“target cells”). In the example shown, 35,2% of the cells were defined as podocytes (D″) and 6.6% of the cells were defined as parietal cells (E″). F, G. The purity of enriched primary podocytes (F, F′) and parietal cells (G, G′) was evaluated after six passages in culture. Staining for the genetic marker beta-gal (X-gal) revealed that virtually all of the cells were either derived from podocytes or parietal cells (merged images: phase contrast+X-gal stainings). H, I. Similarly, in a FACscan analysis, virtually all cells were positive for the genetic tag beta-galactosidase (as revealed by metabolic labeling with FDG). In this representative analysis, 97.1% and 99.4% of the primary podocytes or PECs were defined as beta-gal positive, respectively.</p>", "links"=>[], "tags"=>["cultures", "murine", "podocytes", "parietal"], "article_id"=>322881, "categories"=>["Biological Sciences", "Marine Biology", "Cell Biology", "Genetics", "Physiology"], "users"=>["Nazanin Kabgani", "Tamara Grigoleit", "Kevin Schulte", "Antonio Sechi", "Sibille Sauer-Lehnen", "Carmen Tag", "Peter Boor", "Christoph Kuppe", "Gregor Warsow", "Sandra Schordan", "Jörg Mostertz", "Ravi Kumar Chilukoti", "Georg Homuth", "Nicole Endlich", "Frank Tacke", "Ralf Weiskirchen", "Georg Fuellen", "Karlhans Endlich", "Jürgen Floege", "Bart Smeets", "Marcus J. Moeller"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0034907.g002", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Generation_of_primary_cultures_of_murine_podocytes_or_parietal_cells_/322881", "title"=>"Generation of primary cultures of murine podocytes or parietal cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-04-18 00:48:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/652163"], "description"=>"<p>A–E. Outgrowths from isolated capsulated glomeruli of PEC-rtTA/LC1/R26R mice are predominantly derived from genetically tagged parietal cells. A. Genetic map of triple transgenic PEC-rtTA/LC1/R26R mice (cPodxl, rabbit podocalyxin promoter; rtTA, reverse tetracycline transactivator; Dox, doxycycline). B. Phase contrast image of cellular outgrowths from two capsulated glomeruli. C. Cellular outgrowths are derived from parietal cells as shown by X-gal staining (arrowhead). Within the glomerulus, magnetic beads used for isolation are still visible. D. Genetically tagged parietal cells at later time points (X-Gal staining, after 7 days of culture). E. After six passages, genetically tagged parietal cells show a spindle-shaped morphology (arrows; X-Gal crystals, arrowheads). F. A mixed culture of untagged primary podocytes and genetically tagged primary parietal cells was established to compare the morphology of the two cell types. Beta-gal-negative podocytes formed mostly larger cells (arrowheads) compared to beta-gal-positive parietal cells, which were mostly spindle-shaped (arrows, X-gal staining). G. Stills from time-lapse video (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034907#pone.0034907.s002\" target=\"_blank\">Video S1</a>, seconds indicated on lower right, 1 sec. = 75 min. capture time) of pure cultured parietal cells (see below). Arrowhead indicates a cell transitioning between spindle-shaped and polygonal morphology. H–J. Cellular outgrowths from decapsulated glomeruli of genetically tagged Pod-rtTA/LC1/R26R mice (after 7 or 10 days). H. Genetic map of the transgenic mice. I–J. In most cases, primary podocytes (blue) show a distinct morphology (larger cell body with prominent cytoplasm, arrowhead). For comparison, other cells without a genetic tag can be seen (J, arrow).</p>", "links"=>[], "tags"=>["lineage", "tracing", "cellular"], "article_id"=>322626, "categories"=>["Biological Sciences", "Marine Biology", "Cell Biology", "Genetics", "Physiology"], "users"=>["Nazanin Kabgani", "Tamara Grigoleit", "Kevin Schulte", "Antonio Sechi", "Sibille Sauer-Lehnen", "Carmen Tag", "Peter Boor", "Christoph Kuppe", "Gregor Warsow", "Sandra Schordan", "Jörg Mostertz", "Ravi Kumar Chilukoti", "Georg Homuth", "Nicole Endlich", "Frank Tacke", "Ralf Weiskirchen", "Georg Fuellen", "Karlhans Endlich", "Jürgen Floege", "Bart Smeets", "Marcus J. Moeller"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0034907.g001", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cell_lineage_tracing_of_cellular_outgrowths_/322626", "title"=>"Cell lineage tracing of cellular outgrowths.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-04-18 00:43:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/652766"], "description"=>"<p>PECs were negative for the endothelial marker <i>von Willebrand factor</i> (vWT, A) and for myofibroblast marker <i>alpha-SMA</i> (B). HUVECs (A′) or human dermal fibroblasts (B′) were used as positive controls. Polyclonal parietal cells were positive for claudin-1 (C) and caveolin-1 (D). C′, D′. Negative controls were performed using isotype-matched irrelevant primary antibodies. E. A significantly lower expression of synaptopodin was observed in parietal cells expressed even after six passages compared to primary podocytes (E′) or an immortalized podocyte cell line IHPC (E″). The findings were confirmed by SDS-page with subsequent immunoblotting using lysates of parietal cell cultures 2, 8 and 11 (F–H). Lysates of polyclonal primary podocyte cultures as well as of an immortalized podocyte cell line IHPC also showed expression of claudin-1 and calveolin-1. No expression of caveolin-1 was observed in Hel-1, GDM or Set-1 cell lines (G). Equal loading was verified by Ponceau S stain. H. Podocin expression is absent in primary PECs and down regulated in primary podocyte cells and in an immortalized podocyte cell line IHPC. I. Podoplanin is differentially expressed in primary podocytes relative to primary PECs after six passages in culture (arrow), consistent with mRNA expression analysis. Lysates of isolated glomeruli are used as positive control (H, I).</p>", "links"=>[], "tags"=>["pec"], "article_id"=>323235, "categories"=>["Biological Sciences", "Marine Biology", "Cell Biology", "Genetics", "Physiology"], "users"=>["Nazanin Kabgani", "Tamara Grigoleit", "Kevin Schulte", "Antonio Sechi", "Sibille Sauer-Lehnen", "Carmen Tag", "Peter Boor", "Christoph Kuppe", "Gregor Warsow", "Sandra Schordan", "Jörg Mostertz", "Ravi Kumar Chilukoti", "Georg Homuth", "Nicole Endlich", "Frank Tacke", "Ralf Weiskirchen", "Georg Fuellen", "Karlhans Endlich", "Jürgen Floege", "Bart Smeets", "Marcus J. Moeller"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0034907.g004", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Characterization_of_the_primary_PEC_lines_/323235", "title"=>"Characterization of the primary PEC lines.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-04-18 00:53:55"}
  • {"files"=>["https://ndownloader.figshare.com/files/653179"], "description"=>"<p>Parietal cells were fluorescently labeled in transgenic PEC-rtTA/(tetO)-HIST1H2BJ/GFP mice (A–C), which express histone-eGFP in a doxycycline-inducible fashion in parietal cells but not in podocytes. After labeling of parietal cell nuclei was completed in living mice (genotypes are indicated on the right), the glomeruli were isolated and the cellular outgrowths were analyzed. A–A″, eGFP labeled nuclei can be observed within the capsule of a glomerulus (black arrow). Labeled cellular outgrowths can be observed (white arrows). Unlabeled cellular outgrowths – most likely podocytes - originate from a decapsulated glomerulus (arrowhead). Pax2 is expressed within the nucleus by the majority of cellular outgrowths independent of their origin from parietal cells or podocytes (A′). B, Control staining from the same experiment as in A using irrelevant primary antiserum. Background staining can be observed within the three-dimensional structures of the glomeruli and outside the nucleus of some cells. C, Cellular outgrowths from capsulated glomeruli (black arrow) express WT-1. Nuclear expression of WT-1 can be detected in all cellular outgrowths derived from GFP positive parietal cells (arrows). D, Cellular outgrowths from decapsulated glomeruli of Pod-rtTA/Hist1H2BJ/GFP transgenic mice (black arrows) upregulate Pax2 expression. Pax2 is not expressed within the nuclei of early podocyte outgrowths (white arrows with long tails). Pax2 expression is upregulated in podocyte-derived outgrowths with lower nuclear histone-eGFP levels, presumptively because these cells have already undergone cellular divisions (white arrow with short tail). Cellular outgrowths without histone-eGFP labeling (proliferating podocytes or parietal cells) express the highest levels of Pax2 (arrowheads). E, EGFP labeled podocyte-derived cells express WT-1 in a nuclear pattern (white arrow). EGFP negative (and presumably less differentiated) cells express significantly less WT-1 (white arrowhead).</p>", "links"=>[], "tags"=>["pax2", "wt-1", "cellular", "outgrowths", "parietal", "cells"], "article_id"=>323640, "categories"=>["Biological Sciences", "Marine Biology", "Cell Biology", "Genetics", "Physiology"], "users"=>["Nazanin Kabgani", "Tamara Grigoleit", "Kevin Schulte", "Antonio Sechi", "Sibille Sauer-Lehnen", "Carmen Tag", "Peter Boor", "Christoph Kuppe", "Gregor Warsow", "Sandra Schordan", "Jörg Mostertz", "Ravi Kumar Chilukoti", "Georg Homuth", "Nicole Endlich", "Frank Tacke", "Ralf Weiskirchen", "Georg Fuellen", "Karlhans Endlich", "Jürgen Floege", "Bart Smeets", "Marcus J. Moeller"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0034907.g006", "stats"=>{"downloads"=>0, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_of_Pax2_and_WT_1_in_cellular_outgrowths_of_primary_parietal_cells_and_podocytes_/323640", "title"=>"Expression of Pax2 and WT-1 in cellular outgrowths of primary parietal cells and podocytes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-04-18 01:00:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/652959"], "description"=>"<p>A. Lysates of primary PECs or podocytes were subjected to immunoblotting for Pax2 expression. Primary PECs expressed significant amounts of Pax2, lower amounts were detected in primary podocytes (arrow). Lysates of endothelial cells (HUVEC or glomerular endothelial cells) were used as negative controls. B. After six passages, Pax2 was expressed in primary PECs and podocytes (arrow). Total kidney lysates were used as positive controls, endothelial cells were used as negative controls. C, C′ In immunofluorescent stainings on primary PECs and podocytes after six passages of culture, Pax2 was expressed in a nuclear fashion. Isotype-matched irrelevant antiserum was used as control (C′). D. Immunoblotting lysates of primary PECs or podocytes using ab sc192 showed that WT-1 is expressed in both cell types with significantly higher levels in primary podocytes (arrow). Lysates of endothelial cells were negative for WT1. E. After six passages, WT1 was expressed both in PECs and podocytes in a nuclear fashion (immunofluorescent staining using ab sc192). F. Parietal cells express low levels of WT1 also <i>in vivo</i> in mice with a mixed genetic background using ab sc846 (F, F′, arrowheads, immunohistological staining). For comparison, a strong expression of WT-1 was detected in podocytes (arrows). No WT-1 expression was noted elsewhere in the renal cortex, specifically not in proximal tubular cells (white arrowheads). G. Similarly, PECs expressed WT-1 also in other mouse genetic backgrounds (Sv129; WT-1/PAS staining) H, I. PECs (arrowheads) expressed low amounts of WT-1 also in normal Wistar rats (H) and humans (I). H′. Isotype matched controls were negative.</p>", "links"=>[], "tags"=>["podocyte", "proteins", "parietal"], "article_id"=>323427, "categories"=>["Biological Sciences", "Marine Biology", "Cell Biology", "Genetics", "Physiology"], "users"=>["Nazanin Kabgani", "Tamara Grigoleit", "Kevin Schulte", "Antonio Sechi", "Sibille Sauer-Lehnen", "Carmen Tag", "Peter Boor", "Christoph Kuppe", "Gregor Warsow", "Sandra Schordan", "Jörg Mostertz", "Ravi Kumar Chilukoti", "Georg Homuth", "Nicole Endlich", "Frank Tacke", "Ralf Weiskirchen", "Georg Fuellen", "Karlhans Endlich", "Jürgen Floege", "Bart Smeets", "Marcus J. Moeller"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0034907.g005", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_of_podocyte_marker_proteins_by_primary_parietal_or_podocyte_cell_lines_/323427", "title"=>"Expression of podocyte marker proteins by primary parietal or podocyte cell lines.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-04-18 00:57:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/652583"], "description"=>"<p>A. Eight transcriptomes were determined from two independent primary podocyte cultures and two independent primary PEC cultures grown in two different media (EGM-MV+20% FCS or RPMI+10% FCS), respectively. Transcriptomes were subjected to principal component analysis. PEC cultures (red spheres) are separated from podocyte cultures (blue spheres) along the axis of principle component 1 (PC1), which accounts for 47.8% of the overall variance in gene expression. Cells cultured in RPMI (light red and light blue spheres) segregate from those cultured in EGM-MV (bright red and bright blue spheres) along the axis of PC2, accounting for 11.8% of the variance. B. Primary podocyte and PEC cultures can also be clearly distinguished by cluster analysis of 2,507 significantly differentially regulated genes. C. Differential expression of the gene mRNA transcripts cadherin-3, EPH receptor A7, ladinin and scinderin in primary PEC cultures correlated with preferential expression in PECs versus podocytes in human kidney <i>in vivo</i> (arrowheads; images from <a href=\"http://www.proteinatlas.org\" target=\"_blank\">www.proteinatlas.org</a><a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034907#pone.0034907-Uhlen1\" target=\"_blank\">[16]</a>). D. Expression levels of selected genes in primary podocyte and PEC cultures. Expression levels are given as mean logarithmic values to the base 2 of arbitrary intensity units.</p>", "links"=>[], "tags"=>["podocyte", "pec"], "article_id"=>323052, "categories"=>["Biological Sciences", "Marine Biology", "Cell Biology", "Genetics", "Physiology"], "users"=>["Nazanin Kabgani", "Tamara Grigoleit", "Kevin Schulte", "Antonio Sechi", "Sibille Sauer-Lehnen", "Carmen Tag", "Peter Boor", "Christoph Kuppe", "Gregor Warsow", "Sandra Schordan", "Jörg Mostertz", "Ravi Kumar Chilukoti", "Georg Homuth", "Nicole Endlich", "Frank Tacke", "Ralf Weiskirchen", "Georg Fuellen", "Karlhans Endlich", "Jürgen Floege", "Bart Smeets", "Marcus J. Moeller"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0034907.g003", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Transcriptome_analysis_of_primary_podocyte_and_PEC_cultures_/323052", "title"=>"Transcriptome analysis of primary podocyte and PEC cultures.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-04-18 00:50:52"}
  • {"files"=>["https://ndownloader.figshare.com/files/335120", "https://ndownloader.figshare.com/files/335252"], "description"=>"<div><p>Parietal epithelial cells (PECs) are crucially involved in the pathogenesis of rapidly progressive glomerulonephritis (RPGN) as well as in focal and segmental glomerulosclerosis (FSGS). In this study, transgenic mouse lines were used to isolate pure, genetically tagged primary cultures of PECs or podocytes using FACsorting. By this approach, the morphology of primary glomerular epithelial cells in culture could be resolved: Primary podocytes formed either large cells with intracytoplasmatic extensions or smaller spindle shaped cells, depending on specific culture conditions. Primary PECs were small and exhibited a spindle-shaped or polygonal morphology. In the very early phases of primary culture, rapid changes in gene expression (e.g. of WT-1 and Pax-2) were observed. However, after prolonged culture primary PECs and podocytes still segregated clearly in a transcriptome analysis - demonstrating that the origin of primary cell cultures is important. Of the classical markers, synaptopodin and podoplanin expression were differentially regulated the most in primary PEC and podocyte cultures. However, no expression of any endogenous gene allowed to differentiate between the two cell types in culture. Finally, we show that the transcription factor WT1 is also expressed by PECs. In summary, genetic tagging of PECs and podocytes is a novel and necessary tool to derive pure primary cultures with proven origin. These cultures will be a powerful tool for the emerging field of parietal epithelial cell biology.</p> </div>", "links"=>[], "tags"=>["cultures", "glomerular", "parietal", "epithelial", "cells", "podocytes", "proven"], "article_id"=>126190, "categories"=>["Biological Sciences", "Marine Biology", "Cell Biology", "Genetics", "Physiology"], "users"=>["Nazanin Kabgani", "Tamara Grigoleit", "Kevin Schulte", "Antonio Sechi", "Sibille Sauer-Lehnen", "Carmen Tag", "Peter Boor", "Christoph Kuppe", "Gregor Warsow", "Sandra Schordan", "Jörg Mostertz", "Ravi Kumar Chilukoti", "Georg Homuth", "Nicole Endlich", "Frank Tacke", "Ralf Weiskirchen", "Georg Fuellen", "Karlhans Endlich", "Jürgen Floege", "Bart Smeets", "Marcus J. Moeller"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0034907.s001", "https://dx.doi.org/10.1371/journal.pone.0034907.s002"], "stats"=>{"downloads"=>2, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Primary_Cultures_of_Glomerular_Parietal_Epithelial_Cells_or_Podocytes_with_Proven_Origin/126190", "title"=>"Primary Cultures of Glomerular Parietal Epithelial Cells or Podocytes with Proven Origin", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-04-18 01:43:10"}

PMC Usage Stats | Further Information

  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"12", "year"=>"2012", "pdf"=>"9", "unique-ip"=>"12", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"100", "unique-ip"=>"99", "pdf"=>"68", "year"=>"2012", "figure"=>"109", "scanned-summary"=>"0", "supp-data"=>"5"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"74", "year"=>"2012", "pdf"=>"53", "unique-ip"=>"68", "figure"=>"20", "scanned-summary"=>"0", "supp-data"=>"8"}
  • {"unique-ip"=>"43", "full-text"=>"37", "pdf"=>"29", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"18", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"40", "full-text"=>"42", "pdf"=>"26", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"37", "full-text"=>"36", "pdf"=>"22", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"34", "full-text"=>"41", "pdf"=>"22", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"43", "full-text"=>"53", "pdf"=>"13", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"34", "full-text"=>"35", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"32", "full-text"=>"31", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"19", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"27", "full-text"=>"33", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"28", "full-text"=>"27", "pdf"=>"24", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"32", "full-text"=>"29", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"31", "full-text"=>"36", "pdf"=>"19", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"23", "full-text"=>"24", "pdf"=>"16", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"19", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"23", "full-text"=>"18", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"44", "full-text"=>"38", "pdf"=>"24", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"21", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"20", "full-text"=>"21", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"26", "full-text"=>"23", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"31", "full-text"=>"23", "pdf"=>"16", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"33", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"21", "full-text"=>"21", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"32", "full-text"=>"29", "pdf"=>"11", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"19", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"20", "full-text"=>"20", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"18", "full-text"=>"17", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"25", "full-text"=>"28", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"32", "full-text"=>"31", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"26", "full-text"=>"39", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"28", "full-text"=>"32", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"28", "full-text"=>"27", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"28", "full-text"=>"25", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"27", "full-text"=>"28", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"25", "full-text"=>"30", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"28", "full-text"=>"29", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"4", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"28", "full-text"=>"27", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"20", "full-text"=>"13", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"20", "full-text"=>"17", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"32", "full-text"=>"30", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"19", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"20", "full-text"=>"16", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"21", "full-text"=>"20", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"22", "full-text"=>"20", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"28", "full-text"=>"26", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"24", "full-text"=>"26", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"19", "full-text"=>"19", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"35", "full-text"=>"41", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"24", "full-text"=>"28", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"35", "full-text"=>"27", "pdf"=>"27", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"29", "full-text"=>"28", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"19", "full-text"=>"18", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"46", "full-text"=>"45", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"35", "full-text"=>"33", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"30", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"31", "full-text"=>"33", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"25", "full-text"=>"29", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"31", "full-text"=>"32", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"39", "full-text"=>"36", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"34", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"37", "full-text"=>"40", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"33", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"33", "full-text"=>"33", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"26", "full-text"=>"36", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"28", "full-text"=>"31", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"24", "full-text"=>"30", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"19", "full-text"=>"23", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"30", "full-text"=>"29", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"19", "full-text"=>"20", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"19", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"18", "full-text"=>"16", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"25", "full-text"=>"28", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"28", "full-text"=>"29", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"35", "full-text"=>"39", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"24", "full-text"=>"25", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"27", "full-text"=>"24", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"29", "full-text"=>"29", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"18", "full-text"=>"22", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"30", "full-text"=>"37", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"30", "full-text"=>"33", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"27", "full-text"=>"29", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"23", "full-text"=>"24", "pdf"=>"10", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"26", "full-text"=>"24", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"26", "full-text"=>"35", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"26", "full-text"=>"28", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"30", "full-text"=>"33", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"37", "full-text"=>"42", "pdf"=>"9", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"35", "full-text"=>"42", "pdf"=>"10", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"28", "full-text"=>"27", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"30", "full-text"=>"35", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"26", "full-text"=>"25", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"51", "full-text"=>"49", "pdf"=>"15", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"25", "full-text"=>"28", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"19", "full-text"=>"27", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"16", "full-text"=>"25", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"36", "full-text"=>"32", "pdf"=>"10", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"15", "full-text"=>"19", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"26", "full-text"=>"28", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2020", "month"=>"8"}
  • {"unique-ip"=>"22", "full-text"=>"28", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"9"}
  • {"unique-ip"=>"23", "full-text"=>"32", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2020", "month"=>"10"}
  • {"unique-ip"=>"24", "full-text"=>"26", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"11"}
  • {"unique-ip"=>"23", "full-text"=>"21", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"12"}

Relative Metric

{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Anatomy", "average_usage"=>[308, 530, 649, 751, 844, 933, 1021, 1107, 1194, 1277, 1357, 1426, 1501, 1572, 1634, 1702, 1768, 1838, 1910, 1976, 2046, 2114, 2181, 2241, 2298]}, {"subject_area"=>"/Biology and life sciences/Anatomy and physiology", "average_usage"=>[307, 540, 660, 756, 847, 936, 1025, 1111, 1199, 1282, 1361, 1432]}, {"subject_area"=>"/Biology and life sciences/Computational biology", "average_usage"=>[375, 629, 760, 889, 1000, 1110, 1203, 1298, 1399, 1492, 1603, 1699, 1774, 1855, 1918, 1998, 2062, 2152, 2227, 2312, 2378, 2461, 2528, 2600, 2664]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[312, 547, 668, 769, 861, 953, 1046, 1135, 1224, 1307, 1388, 1464, 1536, 1606, 1676, 1744, 1812, 1882, 1954, 2020, 2089, 2155, 2218, 2282, 2344]}, {"subject_area"=>"/Medicine and health sciences/Anatomy", "average_usage"=>[307, 529, 649, 751, 845, 936, 1025, 1113, 1197, 1284, 1360, 1432, 1501, 1570, 1632, 1697, 1765, 1834, 1904, 1972, 2041, 2107, 2170, 2231, 2292]}]}
Loading … Spinner
There are currently no alerts