Brewhouse-Resident Microbiota Are Responsible for Multi-Stage Fermentation of American Coolship Ale
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Brewhouse-resident microbiota are responsible for multi-stage fermentation of American coolship ale", "type"=>"journal", "authors"=>[{"first_name"=>"Nicholas A.", "last_name"=>"Bokulich", "scopus_author_id"=>"55053326800"}, {"first_name"=>"Charles W.", "last_name"=>"Bamforth", "scopus_author_id"=>"7003538762"}, {"first_name"=>"David A.", "last_name"=>"Mills", "scopus_author_id"=>"35426334600"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84860006840", "sgr"=>"84860006840", "pui"=>"364660945", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pmid"=>"22530036", "doi"=>"10.1371/journal.pone.0035507"}, "id"=>"4775bcc3-2b0a-34a0-8e49-b3d5105b0c98", "abstract"=>"American coolship ale (ACA) is a type of spontaneously fermented beer that employs production methods similar to traditional Belgian lambic. In spite of its growing popularity in the American craft-brewing sector, the fermentation microbiology of ACA has not been previously described, and thus the interface between production methodology and microbial community structure is unexplored. Using terminal restriction fragment length polymorphism (TRFLP), barcoded amplicon sequencing (BAS), quantitative PCR (qPCR) and culture-dependent analysis, ACA fermentations were shown to follow a consistent fermentation progression, initially dominated by Enterobacteriaceae and a range of oxidative yeasts in the first month, then ceding to Saccharomyces spp. and Lactobacillales for the following year. After one year of fermentation, Brettanomyces bruxellensis was the dominant yeast population (occasionally accompanied by minor populations of Candida spp., Pichia spp., and other yeasts) and Lactobacillales remained dominant, though various aerobic bacteria became more prevalent. This work demonstrates that ACA exhibits a conserved core microbial succession in absence of inoculation, supporting the role of a resident brewhouse microbiota. These findings establish this core microbial profile of spontaneous beer fermentations as a target for production control points and quality standards for these beers.", "link"=>"http://www.mendeley.com/research/brewhouseresident-microbiota-responsible-multistage-fermentation-american-coolship-ale", "reader_count"=>162, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>4, "Researcher"=>31, "Student > Doctoral Student"=>7, "Student > Ph. D. Student"=>28, "Student > Postgraduate"=>9, "Other"=>15, "Student > Master"=>39, "Student > Bachelor"=>21, "Lecturer"=>1, "Professor"=>5}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>4, "Researcher"=>31, "Student > Doctoral Student"=>7, "Student > Ph. D. Student"=>28, "Student > Postgraduate"=>9, "Other"=>15, "Student > Master"=>39, "Student > Bachelor"=>21, "Lecturer"=>1, "Professor"=>5}, "reader_count_by_subject_area"=>{"Unspecified"=>6, "Agricultural and Biological Sciences"=>112, "Arts and Humanities"=>1, "Chemistry"=>5, "Earth and Planetary Sciences"=>2, "Engineering"=>5, "Environmental Science"=>4, "Biochemistry, Genetics and Molecular Biology"=>13, "Nursing and Health Professions"=>1, "Materials Science"=>1, "Medicine and Dentistry"=>6, "Neuroscience"=>1, "Immunology and Microbiology"=>5}, "reader_count_by_subdiscipline"=>{"Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>6}, "Unspecified"=>{"Unspecified"=>6}, "Environmental Science"=>{"Environmental Science"=>4}, "Arts and Humanities"=>{"Arts and Humanities"=>1}, "Engineering"=>{"Engineering"=>5}, "Chemistry"=>{"Chemistry"=>5}, "Neuroscience"=>{"Neuroscience"=>1}, "Earth and Planetary Sciences"=>{"Earth and Planetary Sciences"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>5}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>112}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>13}}, "reader_count_by_country"=>{"Canada"=>4, "Netherlands"=>1, "Sweden"=>1, "Burkina Faso"=>1, "United States"=>7, "Brazil"=>1, "Mexico"=>1, "Chile"=>1, "France"=>2, "Australia"=>1}, "group_count"=>8}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84860006840"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84860006840?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84860006840&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84860006840&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84860006840", "dc:identifier"=>"SCOPUS_ID:84860006840", "eid"=>"2-s2.0-84860006840", "dc:title"=>"Brewhouse-resident microbiota are responsible for multi-stage fermentation of American coolship ale", "dc:creator"=>"Bokulich N.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"7", "prism:issueIdentifier"=>"4", "prism:pageRange"=>nil, "prism:coverDate"=>"2012-04-18", "prism:coverDisplayDate"=>"18 April 2012", "prism:doi"=>"10.1371/journal.pone.0035507", "citedby-count"=>"101", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"University of California, Davis", "affiliation-city"=>"Davis", "affiliation-country"=>"United States"}], "pubmed-id"=>"22530036", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e35507", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0035507", "share_count"=>2, "like_count"=>3, "comment_count"=>0, "click_count"=>0, "total_count"=>5}

Twitter

Counter

  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"88", "xml_views"=>"10", "html_views"=>"1125"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"51", "xml_views"=>"1", "html_views"=>"748"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"144"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"28", "xml_views"=>"0", "html_views"=>"256"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"25", "xml_views"=>"4", "html_views"=>"99"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"96"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"37", "xml_views"=>"0", "html_views"=>"300"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"37", "xml_views"=>"3", "html_views"=>"340"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"148"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"195"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"22", "xml_views"=>"4", "html_views"=>"220"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"315"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"34", "xml_views"=>"1", "html_views"=>"184"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"28", "xml_views"=>"1", "html_views"=>"178"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"127"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"23", "xml_views"=>"1", "html_views"=>"130"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"136"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"255"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"275"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"26", "xml_views"=>"2", "html_views"=>"229"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"121"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"34", "xml_views"=>"0", "html_views"=>"172"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"34", "xml_views"=>"0", "html_views"=>"767"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"19", "xml_views"=>"2", "html_views"=>"275"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"84", "xml_views"=>"1", "html_views"=>"212"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"42", "xml_views"=>"0", "html_views"=>"213"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"140"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"33", "xml_views"=>"0", "html_views"=>"139"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"23", "xml_views"=>"2", "html_views"=>"108"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"24", "xml_views"=>"1", "html_views"=>"165"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"29", "xml_views"=>"1", "html_views"=>"156"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"163"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"172"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"143"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"33", "xml_views"=>"0", "html_views"=>"247"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"74", "xml_views"=>"0", "html_views"=>"221"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"71", "xml_views"=>"2", "html_views"=>"233"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"36", "xml_views"=>"1", "html_views"=>"182"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"40", "xml_views"=>"0", "html_views"=>"170"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"47", "xml_views"=>"0", "html_views"=>"187"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"36", "xml_views"=>"4", "html_views"=>"163"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"169"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"271"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"190"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"264"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"230"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"208"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"244"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"30", "xml_views"=>"0", "html_views"=>"224"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"157"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"169"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"118"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"109"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"32", "xml_views"=>"0", "html_views"=>"94"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"31", "xml_views"=>"0", "html_views"=>"219"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"269"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"18", "xml_views"=>"1", "html_views"=>"291"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"23", "xml_views"=>"2", "html_views"=>"331"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"296"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"423"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"22", "xml_views"=>"3", "html_views"=>"494"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"353"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"383"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"2", "html_views"=>"257"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"271"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"28", "xml_views"=>"1", "html_views"=>"304"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"331"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"44", "xml_views"=>"1", "html_views"=>"425"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"21", "xml_views"=>"3", "html_views"=>"449"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"297"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"32", "xml_views"=>"0", "html_views"=>"71"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"36", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"67", "xml_views"=>"0", "html_views"=>"75"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"34", "xml_views"=>"0", "html_views"=>"68"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"29", "xml_views"=>"2", "html_views"=>"51"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"22", "xml_views"=>"6", "html_views"=>"53"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"23", "xml_views"=>"1", "html_views"=>"46"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"20", "xml_views"=>"2", "html_views"=>"38"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"23", "xml_views"=>"1", "html_views"=>"56"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"36"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"37", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"34"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"43", "xml_views"=>"0", "html_views"=>"92"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"199", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"265", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"275", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"218", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"206", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"89", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"48", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"16", "xml_views"=>"2", "html_views"=>"27"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"25", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"33", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"80", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"87", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"40", "xml_views"=>"0", "html_views"=>"14"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/652388"], "description"=>"*<p>Indicates the time points (wk) at which colonies matching the morphotype of this organism were cultured. All colony morphotypes were isolated in duplicate for sequencing and identification.</p>", "links"=>[], "tags"=>["assignments"], "article_id"=>322880, "categories"=>["Biological Sciences", "Biochemistry", "Microbiology"], "users"=>["Nicholas A. Bokulich", "Charles W. Bamforth", "David A. Mills"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0035507.t001", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Taxonomic_Assignments_of_Bacteria_Isolated_from_ACA_/322880", "title"=>"Taxonomic Assignments of Bacteria Isolated from ACA.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-04-18 00:48:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/651695"], "description"=>"<p>Normalized relative OTU peak areas for multiple ACA fermentations observed over a 3-year period by ITS-TRFLP (yeasts, <i>Panel A</i>), 16S-TRFLP (bacteria, <i>Panel B</i>), and LAB-TRFLP (lactic acid bacteria, <i>Panel C</i>). All samples were tested in duplicate, when possible, and each bar represents averaged duplicates for a single time point, single barrel. <i>y</i>-axes indicate relative OTU abundance. *Sample was not amplifiable with these specific primers.</p>", "links"=>[], "tags"=>["aca", "fermentation", "Succession"], "article_id"=>322189, "categories"=>["Biological Sciences", "Biochemistry", "Microbiology"], "users"=>["Nicholas A. Bokulich", "Charles W. Bamforth", "David A. Mills"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0035507.g002", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_TRFLP_analysis_of_ACA_fermentation_succession_across_complete_timescale_/322189", "title"=>"TRFLP analysis of ACA fermentation succession across complete timescale.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-04-18 00:36:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/651599"], "description"=>"<p><i>Panel A</i>: Sampling regime employed representing 3 years of ACA fermentation. White bars represent the span of sampling times for each batch. Labels indicate batch number (number of barrel replicates in parenthesis). <i>Panels B/C</i>: Real-time PCR of total bacteria and total yeast populations (<i>Panel B</i>) and pH and °Plato (<i>Panel C</i>) across ACA fermentation. All values are averages of multiple batches tested in duplicate (where possible; <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035507#pone.0035507.s001\" target=\"_blank\">Figure S1</a>). Error bars indicate ±1 standard deviation. ◊, Total bacteria; □, total yeasts; ▵, pH; ○, °Plato. Arrow indicates time at which fruit was added and beer was refermented in bottle.</p>", "links"=>[], "tags"=>["fermentation", "sampling"], "article_id"=>322092, "categories"=>["Biological Sciences", "Biochemistry", "Microbiology"], "users"=>["Nicholas A. Bokulich", "Charles W. Bamforth", "David A. Mills"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0035507.g001", "stats"=>{"downloads"=>1, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ACA_fermentation_profile_and_sampling_regime_/322092", "title"=>"ACA fermentation profile and sampling regime.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-04-18 00:34:52"}
  • {"files"=>["https://ndownloader.figshare.com/files/652136"], "description"=>"<p>Comparison of universal bacterial community structure (A,B,E,F) and <i>Lactobacillales</i> (C,D,G,H) in Spring batch 7 (<i>right</i>) and Winter batch 8 (<i>left</i>). <i>Panel A/E</i>: Relative abundance of 16S-TRFLP bacterial OTUs. <i>Panel B/F</i>: Class-level abundance per sample as percentage of total BAS sequences. <i>Panel C/G</i>: Relative abundance of LAB-TRFLP OTUs. <i>Panel D/H</i>: Genus-level relative abundance of <i>Lactobacillales</i> BAS sequences. All TRFLP samples are averages of duplicate samples. <i>y</i>-axes indicate relative OTU abundance.</p>", "links"=>[], "tags"=>["aca", "batch"], "article_id"=>322633, "categories"=>["Biological Sciences", "Biochemistry", "Microbiology"], "users"=>["Nicholas A. Bokulich", "Charles W. Bamforth", "David A. Mills"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0035507.g005", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Parallel_ACA_batch_comparison_/322633", "title"=>"Parallel ACA batch comparison.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-04-18 00:43:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/651997"], "description"=>"<p><i>Panel A</i>: Class-level taxon relative abundance per sample as a percentage of total sequences. <i>Panel B</i>: Genus-level relative abundance of <i>Lactobacillales</i>. All bars represent a single sample from a single batch, and most time points are represented by at least two independent samples (where available), as presented. <i>y</i>-axes indicate relative OTU abundance.</p>", "links"=>[], "tags"=>["taxa", "abundance", "bas"], "article_id"=>322490, "categories"=>["Biological Sciences", "Biochemistry", "Microbiology"], "users"=>["Nicholas A. Bokulich", "Charles W. Bamforth", "David A. Mills"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0035507.g004", "stats"=>{"downloads"=>2, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Bacterial_taxa_abundance_measured_using_BAS_5_8242_sequences_/322490", "title"=>"Bacterial taxa abundance measured using BAS 5′ sequences.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-04-18 00:41:30"}
  • {"files"=>["https://ndownloader.figshare.com/files/652305"], "description"=>"<p>PCoA of Bray-Curtis dissimilarity scores derived from 16S-TRFLP of universal bacterial communities (<i>Panel A</i>) and ITS-TRFLP of yeasts (<i>Panel B</i>). Samples are colored by age (wk), as indicated by adjacent number. Sample distance is a function of shared OTU similarity. <i>Panel C</i>: 3-dimensional PCoA of weighted UniFrac distance of BAS of ACA samples. Samples are colored by age (wk), as indicated by adjacent number, their distance within the 3-dimensional space being a function of the phylogenetic similarity and abundance of their constituent taxa. Grey bubbles represent correlation of loadings (as taxonomic groups) along the same coordinates; their placement explains how much variance along each PC is explained by these taxa, with size as a function of relative abundance.</p>", "links"=>[], "tags"=>["coordinates", "aca", "microbial"], "article_id"=>322801, "categories"=>["Biological Sciences", "Biochemistry", "Microbiology"], "users"=>["Nicholas A. Bokulich", "Charles W. Bamforth", "David A. Mills"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0035507.g006", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Principal_coordinates_analysis_of_ACA_microbial_succession_/322801", "title"=>"Principal coordinates analysis of ACA microbial succession.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-04-18 00:46:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/334863", "https://ndownloader.figshare.com/files/334950", "https://ndownloader.figshare.com/files/334993", "https://ndownloader.figshare.com/files/335055"], "description"=>"<div><p>American coolship ale (ACA) is a type of spontaneously fermented beer that employs production methods similar to traditional Belgian lambic. In spite of its growing popularity in the American craft-brewing sector, the fermentation microbiology of ACA has not been previously described, and thus the interface between production methodology and microbial community structure is unexplored. Using terminal restriction fragment length polymorphism (TRFLP), barcoded amplicon sequencing (BAS), quantitative PCR (qPCR) and culture-dependent analysis, ACA fermentations were shown to follow a consistent fermentation progression, initially dominated by <em>Enterobacteriaceae</em> and a range of oxidative yeasts in the first month, then ceding to <em>Saccharomyces</em> spp. and <em>Lactobacillales</em> for the following year. After one year of fermentation, <em>Brettanomyces bruxellensis</em> was the dominant yeast population (occasionally accompanied by minor populations of <em>Candida</em> spp., <em>Pichia</em> spp., and other yeasts) and <em>Lactobacillales</em> remained dominant, though various aerobic bacteria became more prevalent. This work demonstrates that ACA exhibits a conserved core microbial succession in absence of inoculation, supporting the role of a resident brewhouse microbiota. These findings establish this core microbial profile of spontaneous beer fermentations as a target for production control points and quality standards for these beers.</p> </div>", "links"=>[], "tags"=>["brewhouse-resident", "microbiota", "are", "multi-stage", "fermentation", "american", "coolship", "ale"], "article_id"=>126159, "categories"=>["Biological Sciences", "Biochemistry", "Microbiology"], "users"=>["Nicholas A. Bokulich", "Charles W. Bamforth", "David A. Mills"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0035507.s001", "https://dx.doi.org/10.1371/journal.pone.0035507.s002", "https://dx.doi.org/10.1371/journal.pone.0035507.s003", "https://dx.doi.org/10.1371/journal.pone.0035507.s004"], "stats"=>{"downloads"=>17, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Brewhouse_Resident_Microbiota_Are_Responsible_for_Multi_Stage_Fermentation_of_American_Coolship_Ale/126159", "title"=>"Brewhouse-Resident Microbiota Are Responsible for Multi-Stage Fermentation of American Coolship Ale", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-04-18 01:42:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/651850"], "description"=>"<p>Hierarchical relationship among samples based on Euclidean distance of 16S-TRFLP OTU abundance profiles derived from the MspI restriction digest. Node labels (and associated colors) indicate age- and batch-based groups. Tip labels indicate batch number.wk.</p>", "links"=>[], "tags"=>["hierarchical", "clustering", "demonstrates", "age-based", "grouping"], "article_id"=>322340, "categories"=>["Biological Sciences", "Biochemistry", "Microbiology"], "users"=>["Nicholas A. Bokulich", "Charles W. Bamforth", "David A. Mills"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0035507.g003", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_UPGMA_hierarchical_clustering_demonstrates_age_based_grouping_of_samples_/322340", "title"=>"UPGMA hierarchical clustering demonstrates age-based grouping of samples.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-04-18 00:39:00"}

PMC Usage Stats | Further Information

  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"4", "year"=>"2012", "pdf"=>"0", "unique-ip"=>"4", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"76", "unique-ip"=>"74", "pdf"=>"46", "year"=>"2012", "figure"=>"13", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"30", "year"=>"2012", "pdf"=>"9", "unique-ip"=>"31", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"3"}
  • {"unique-ip"=>"23", "full-text"=>"26", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"22", "full-text"=>"23", "pdf"=>"8", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"27", "full-text"=>"33", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"7", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"51", "full-text"=>"50", "pdf"=>"19", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"21", "full-text"=>"21", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"22", "full-text"=>"27", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"33", "full-text"=>"31", "pdf"=>"18", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"25", "full-text"=>"24", "pdf"=>"9", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"4", "pdf"=>"5", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"3", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"22", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"20", "full-text"=>"18", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"9", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"30", "full-text"=>"19", "pdf"=>"34", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"14", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"20", "full-text"=>"24", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"20", "full-text"=>"19", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"28", "full-text"=>"46", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"17", "full-text"=>"28", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"22", "full-text"=>"40", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"16", "full-text"=>"21", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"33", "full-text"=>"40", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"43", "full-text"=>"48", "pdf"=>"18", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"18", "full-text"=>"25", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"32", "full-text"=>"62", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"17", "full-text"=>"35", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"16", "full-text"=>"10", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"22", "full-text"=>"19", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"4", "cited-by"=>"3", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"20", "full-text"=>"15", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"23", "full-text"=>"21", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"18", "full-text"=>"15", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"19", "full-text"=>"37", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"16", "full-text"=>"19", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"18", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"18", "full-text"=>"17", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"22", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"20", "full-text"=>"20", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"14", "full-text"=>"18", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"23", "full-text"=>"24", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"45", "full-text"=>"51", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"18", "full-text"=>"19", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"31", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"24", "full-text"=>"28", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"16", "full-text"=>"21", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"20", "full-text"=>"23", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"21", "full-text"=>"22", "pdf"=>"20", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"3", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"20", "full-text"=>"20", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"20", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"8", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"9", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"26", "full-text"=>"27", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"18", "full-text"=>"25", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"19", "full-text"=>"22", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}

Relative Metric

{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[322, 550, 671, 773, 864, 955, 1048, 1135, 1223, 1308, 1387, 1465, 1534, 1602, 1673, 1744, 1813, 1885, 1955, 2026, 2093, 2160, 2228, 2290, 2349]}, {"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[316, 541, 663, 766, 856, 950, 1041, 1128, 1218, 1302, 1382, 1456, 1526, 1593, 1657, 1729, 1796, 1862, 1930, 1999, 2065, 2132, 2202, 2261, 2319]}, {"subject_area"=>"/Biology and life sciences/Nutrition", "average_usage"=>[292, 539, 673, 776, 876, 986, 1099, 1210, 1305, 1390, 1476, 1560, 1633, 1726, 1813, 1903, 1969, 2024, 2082, 2153, 2235, 2301, 2366, 2432, 2495]}, {"subject_area"=>"/Medicine and health sciences/Nutrition", "average_usage"=>[307, 546, 676, 780, 888, 986, 1099, 1202, 1282, 1376, 1468, 1552, 1627, 1713, 1774, 1875, 1944, 2011, 2079, 2133, 2210, 2282, 2358, 2422, 2480]}]}
Loading … Spinner
There are currently no alerts