Biophysical Assessment of Single Cell Cytotoxicity: Diesel Exhaust Particle-Treated Human Aortic Endothelial Cells
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{"title"=>"Biophysical assessment of single cell cytotoxicity: Diesel exhaust particle-treated human aortic endothelial cells", "type"=>"journal", "authors"=>[{"first_name"=>"Yangzhe", "last_name"=>"Wu", "scopus_author_id"=>"8331384200"}, {"first_name"=>"Tian", "last_name"=>"Yu", "scopus_author_id"=>"54685585600"}, {"first_name"=>"Timothy A.", "last_name"=>"Gilbertson", "scopus_author_id"=>"7005779916"}, {"first_name"=>"Anhong", "last_name"=>"Zhou", "scopus_author_id"=>"7202762964"}, {"first_name"=>"Hao", "last_name"=>"Xu", "scopus_author_id"=>"55350959100"}, {"first_name"=>"Kytai Truong", "last_name"=>"Nguyen", "scopus_author_id"=>"7202572420"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"doi"=>"10.1371/journal.pone.0036885", "scopus"=>"2-s2.0-84861466694", "pui"=>"364882965", "issn"=>"19326203", "pmid"=>"22662129", "sgr"=>"84861466694", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)"}, "id"=>"956350b6-642c-3b99-b41f-7b00cf139511", "abstract"=>"Exposure to diesel exhaust particles (DEPs), a major source of traffic-related air pollution, has become a serious health concern due to its adverse influences on human health including cardiovascular and respiratory disorders. To elucidate the relationship between biophysical properties (cell topography, cytoskeleton organizations, and cell mechanics) and functions of endothelial cells exposed to DEPs, atomic force microscope (AFM) was applied to analyze the toxic effects of DEPs on a model cell line from human aortic endothelial cells (HAECs). Fluorescence microscopy and flow cytometry were also applied to further explore DEP-induced cytotoxicity in HAECs. Results revealed that DEPs could negatively impair cell viability and alter membrane nanostructures and cytoskeleton components in a dosage- and a time-dependent manner; and analyses suggested that DEPs-induced hyperpolarization in HAECs appeared in a time-dependent manner, implying DEP treatment would lead to vasodilation, which could be supported by down-regulation of cell biophysical properties (e.g., cell elasticity). These findings are consistent with the conclusion that DEP exposure triggers important biochemical and biophysical changes that would negatively impact the pathological development of cardiovascular diseases. For example, DEP intervention would be one cause of vasodilation, which will expand understanding of biophysical aspects associated with DEP cytotoxicity in HAECs.", "link"=>"http://www.mendeley.com/research/biophysical-assessment-single-cell-cytotoxicity-diesel-exhaust-particletreated-human-aortic-endothel-2", "reader_count"=>18, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>6, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>2, "Student > Master"=>3, "Lecturer > Senior Lecturer"=>1, "Professor"=>2}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>6, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>2, "Student > Master"=>3, "Lecturer > Senior Lecturer"=>1, "Professor"=>2}, "reader_count_by_subject_area"=>{"Engineering"=>2, "Environmental Science"=>2, "Materials Science"=>1, "Mathematics"=>1, "Medicine and Dentistry"=>4, "Agricultural and Biological Sciences"=>4, "Physics and Astronomy"=>2, "Social Sciences"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>2}, "Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Social Sciences"=>{"Social Sciences"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>4}, "Mathematics"=>{"Mathematics"=>1}, "Environmental Science"=>{"Environmental Science"=>2}}, "reader_count_by_country"=>{"Colombia"=>1, "France"=>1}, "group_count"=>1}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/327313", "https://ndownloader.figshare.com/files/327387", "https://ndownloader.figshare.com/files/327501", "https://ndownloader.figshare.com/files/327693", "https://ndownloader.figshare.com/files/327878", "https://ndownloader.figshare.com/files/327982"], "description"=>"<div><p>Exposure to diesel exhaust particles (DEPs), a major source of traffic-related air pollution, has become a serious health concern due to its adverse influences on human health including cardiovascular and respiratory disorders. To elucidate the relationship between biophysical properties (cell topography, cytoskeleton organizations, and cell mechanics) and functions of endothelial cells exposed to DEPs, atomic force microscope (AFM) was applied to analyze the toxic effects of DEPs on a model cell line from human aortic endothelial cells (HAECs). Fluorescence microscopy and flow cytometry were also applied to further explore DEP-induced cytotoxicity in HAECs. <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036885#s3\">Results</a> revealed that DEPs could negatively impair cell viability and alter membrane nanostructures and cytoskeleton components in a dosage- and a time-dependent manner; and analyses suggested that DEPs-induced hyperpolarization in HAECs appeared in a time-dependent manner, implying DEP treatment would lead to vasodilation, which could be supported by down-regulation of cell biophysical properties (e.g., cell elasticity). These findings are consistent with the conclusion that DEP exposure triggers important biochemical and biophysical changes that would negatively impact the pathological development of cardiovascular diseases. For example, DEP intervention would be one cause of vasodilation, which will expand understanding of biophysical aspects associated with DEP cytotoxicity in HAECs.</p> </div>", "links"=>[], "tags"=>["biophysical", "diesel", "particle-treated", "aortic", "endothelial", "cells"], "article_id"=>124606, "categories"=>["Physics", "Medicine", "Cell Biology", "Biotechnology", "Pharmacology"], "users"=>["Yangzhe Wu", "Tian Yu", "Timothy A. Gilbertson", "Anhong Zhou", "Hao Xu", "Kytai Truong Nguyen"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0036885.s001", "https://dx.doi.org/10.1371/journal.pone.0036885.s002", "https://dx.doi.org/10.1371/journal.pone.0036885.s003", "https://dx.doi.org/10.1371/journal.pone.0036885.s004", "https://dx.doi.org/10.1371/journal.pone.0036885.s005", "https://dx.doi.org/10.1371/journal.pone.0036885.s006"], "stats"=>{"downloads"=>34, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Biophysical_Assessment_of_Single_Cell_Cytotoxicity_Diesel_Exhaust_Particle_Treated_Human_Aortic_Endothelial_Cells/124606", "title"=>"Biophysical Assessment of Single Cell Cytotoxicity: Diesel Exhaust Particle-Treated Human Aortic Endothelial Cells", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-05-25 01:16:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/632757"], "description"=>"<p>Column 1 shows images of untreated (0 µg/ml) group; columns 2–4 are images of cells treated with different concentrations of DEPs for 4 hours. Row 1 shows bright-field images, row 2 is fluorescence images, and row 3 exhibits AFM images of the same cells. Optical images were obtained by the 60× OIL (or 100× OIL for 10 µg/ml group); scanning size of AFM images is marked on the respective images. The black dots representatively pointing by green arrows are DEPs attached on cell membrane.</p>", "links"=>[], "tags"=>["observations", "haecs", "coupled", "microscope"], "article_id"=>303244, "categories"=>["Physics", "Medicine", "Cell Biology", "Biotechnology", "Pharmacology"], "users"=>["Yangzhe Wu", "Tian Yu", "Timothy A. Gilbertson", "Anhong Zhou", "Hao Xu", "Kytai Truong Nguyen"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0036885.g006", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Representative_observations_of_fixed_HAECs_using_the_coupled_AF_FL_microscope_in_PBS_/303244", "title"=>"Representative observations of fixed HAECs using the coupled AF/FL microscope in PBS.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-05-25 00:54:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/633008"], "description"=>"<p>The panel arrangement is similar to <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036885#pone-0036885-g006\" target=\"_blank\">Figure 6</a>. Optical images were obtained using a 100× oil objective. The exposure time of DEPs is marked on respective optical images. It is seen that fluorescence intensity slightly decreased, although cytoskeletal structures can be seen after 48 hours of treatment. Interestingly, cellular mitosis was still progressing and a dividing cell nucleus can be seen (column 4), implying this low dosage did not completely inhibit cell activities, which coincided with assessment of cell viability. Additionally, DEPs attached on cell membrane surface are seen for all four experimental groups, as indicated by green arrows.</p>", "links"=>[], "tags"=>["images", "dep", "-treated", "haecs", "pbs", "coupled"], "article_id"=>303492, "categories"=>["Physics", "Medicine", "Cell Biology", "Biotechnology", "Pharmacology"], "users"=>["Yangzhe Wu", "Tian Yu", "Timothy A. Gilbertson", "Anhong Zhou", "Hao Xu", "Kytai Truong Nguyen"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0036885.g007", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Representative_images_of_DEP_10_181_g_ml_treated_HAECs_fixed_cells_in_PBS_obtained_by_the_coupled_AF_FL_microscope_/303492", "title"=>"Representative images of DEP (10 µg/ml) -treated HAECs (fixed cells) in PBS obtained by the coupled AF/FL microscope.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-05-25 00:58:12"}
  • {"files"=>["https://ndownloader.figshare.com/files/633174"], "description"=>"<p>(a) Representative graphs show that DEP exposure induced a significant decrease in fluorescence intensity of DiBAC4(3). (b) Histograms of changes in fluorescence intensity. Fluorescence intensity of experimental groups is presented as a percentage of control (0 hour) (that of control group was normalized as 1). <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036885#s3\" target=\"_blank\">Results</a> represent the means of three separate experiments, and error bars represent the standard error of the mean. All experimental groups significantly differs from control group (*<i>p</i><0.01).</p>", "links"=>[], "tags"=>["plasma", "membrane", "haecs", "treated", "10", "deps", "assayed"], "article_id"=>303651, "categories"=>["Physics", "Medicine", "Cell Biology", "Biotechnology", "Pharmacology"], "users"=>["Yangzhe Wu", "Tian Yu", "Timothy A. Gilbertson", "Anhong Zhou", "Hao Xu", "Kytai Truong Nguyen"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0036885.g008", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Changes_of_plasma_membrane_potential_of_live_HAECs_treated_with_or_without_10_181_g_ml_DEPs_assayed_by_flow_cytometry_/303651", "title"=>"Changes of plasma membrane potential of live HAECs treated with or without 10 µg/ml DEPs assayed by flow cytometry.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-05-25 01:00:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/632493"], "description"=>"<p>These maps are only to exhibit heterogeneous property in cell mechanics and do not reflect alteration tendency of <i>F</i><sub>ad</sub> or <i>E</i> because of individuality of cell-cell. Column 1, untreated (0 µg/ml) cells; columns 2–4, images of cells treated with different concentrations of DEPs for 4 hours. Row 1 shows AFM deflection mode images; rows 2 and 3 are their corresponding maps of adhesion force and Young's modulus. The color bars showing at the right of maps display the value scale of <i>F</i><sub>ad</sub> and <i>E</i>. The scanning size of AFM images is marked on each image of row 1.</p>", "links"=>[], "tags"=>["mechanics", "adhesion", "modulus", "cells"], "article_id"=>302978, "categories"=>["Physics", "Medicine", "Cell Biology", "Biotechnology", "Pharmacology"], "users"=>["Yangzhe Wu", "Tian Yu", "Timothy A. Gilbertson", "Anhong Zhou", "Hao Xu", "Kytai Truong Nguyen"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0036885.g004", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Maps_of_cell_mechanics_including_adhesion_force_F_ad_nN_and_Young_s_modulus_E_kPa_of_live_HAECs_which_were_measured_on_single_cells_in_culture_medium_/302978", "title"=>"Maps of cell mechanics including adhesion force (<i>F</i><sub>ad</sub>, nN) and Young's modulus (<i>E</i>, kPa) of live HAECs, which were measured on single cells in culture medium.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-05-25 00:49:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/632317"], "description"=>"<p>Column 1 shows images of cells treated with DEPs for 4 hours; column 2, 8 hours; column 3, 24 hours; column 4, 48 hours. Row 1 shows deflection mode images of single cells; rows 2 and 3 show images of membrane surface ultrastructures. Particles on cells representatively indicated by green arrows are DEP. Scanning size of row 1 is marked on respective image; and that of row 2 and row 3 (ultrastructures): 10 µm×10 µm. This group of images indicated that when treatment time increased from 4 hours to 48 hours, cell membrane damage appeared in a time-dependent manner.</p>", "links"=>[], "tags"=>["afm", "deflection", "images", "haecs", "treated", "10", "deps"], "article_id"=>302798, "categories"=>["Physics", "Medicine", "Cell Biology", "Biotechnology", "Pharmacology"], "users"=>["Yangzhe Wu", "Tian Yu", "Timothy A. Gilbertson", "Anhong Zhou", "Hao Xu", "Kytai Truong Nguyen"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0036885.g003", "stats"=>{"downloads"=>4, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Representative_AFM_deflection_mode_images_of_HAECs_treated_with_10_181_g_ml_DEPs_for_different_time_cells_were_fixed_prior_to_observe_in_PBS_/302798", "title"=>"Representative AFM deflection mode images of HAECs treated with 10 µg/ml DEPs for different time (cells were fixed prior to observe in PBS).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-05-25 00:46:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/632096"], "description"=>"<p>Column 1, images of untreated cells (0 µg/ml group); columns 2–4, images of cells treated with different concentrations of DEPs for 4 hours. Row I, image of single cells, whose scanning size is marked on respective image; rows II and III, images of membrane surface ultrastructures, whose scanning size is 10 µm×10 µm. Particles (representatively shown by green arrows) on cells are DEPs. This group of images indicated that when DEP concentration increased from 0 µg/ml to 100 µg/ml, cytoskeletal structures became gradually degraded, suggesting that cell membrane damage appeared in a dosage-dependent manner. And poor resolution of ultra-structures of 100 µg/ml DEP treated cells is mainly resulted from influences of the large amount of DEPs on membrane surface.</p>", "links"=>[], "tags"=>["afm", "deflection", "images", "haecs"], "article_id"=>302584, "categories"=>["Physics", "Medicine", "Cell Biology", "Biotechnology", "Pharmacology"], "users"=>["Yangzhe Wu", "Tian Yu", "Timothy A. Gilbertson", "Anhong Zhou", "Hao Xu", "Kytai Truong Nguyen"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0036885.g002", "stats"=>{"downloads"=>1, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Representative_AFM_deflection_mode_images_of_fixed_HAECs_obtained_in_PBS_/302584", "title"=>"Representative AFM deflection mode images of fixed HAECs obtained in PBS.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-05-25 00:43:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/631853"], "description"=>"<p>HAECs cells were exposed to DEPs with three different concentrations: 10 µg/ml (row 1), 50 µg/ml (row 2), 100 µg/ml (row 3), and four treatment durations: 4 hours (column 1), 8 hours (column 2), 24 hours (column 3), and 48 hours (column 4), respectively. Cells are stained with Invitrogen LIVE/DEAD Viability/Cytotoxicity Assay Kit. Green fluorescence presents live cells, whereas red fluorescence shows dead or membrane-damaged cells. All images were obtained with 10× lens. These fluorescence images together revealed that DEPs impaired cell viability in a dosage- and a time-dependent manner. And the corresponding bright-field picture of each fluorescent image is shown as <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036885#pone.0036885.s005\" target=\"_blank\">Information S5</a>, from which DEPs can be clearly seen.</p>", "links"=>[], "tags"=>["fluorescence", "images", "viability"], "article_id"=>302340, "categories"=>["Physics", "Medicine", "Cell Biology", "Biotechnology", "Pharmacology"], "users"=>["Yangzhe Wu", "Tian Yu", "Timothy A. Gilbertson", "Anhong Zhou", "Hao Xu", "Kytai Truong Nguyen"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0036885.g001", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Representative_fluorescence_images_of_cell_viability_evaluation_/302340", "title"=>"Representative fluorescence images of cell viability evaluation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-05-25 00:39:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/632612"], "description"=>"<p>Decreases of adhesion force implicates depression of cell membrane adhesion behavior in the presence of DEP, and down-regulation of Young's modulus suggests cells become softer in the context of cytoskeleton losing induced by DEP treatment. The data of histograms were obtained from multiple cells (N<sub>cell</sub> = 12 for each group, and 26 datum points on each cell). Error bar: standard error (SE). (*, P<0.01).</p>", "links"=>[], "tags"=>["adhesion", "modulus"], "article_id"=>303103, "categories"=>["Physics", "Medicine", "Cell Biology", "Biotechnology", "Pharmacology"], "users"=>["Yangzhe Wu", "Tian Yu", "Timothy A. Gilbertson", "Anhong Zhou", "Hao Xu", "Kytai Truong Nguyen"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0036885.g005", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Statistical_analysis_of_adhesion_force_F_ad_nN_and_Young_s_Modulus_E_kPa_of_live_HAECs_/303103", "title"=>"Statistical analysis of adhesion force (<i>F</i><sub>ad</sub>, nN) and Young's Modulus (<i>E</i>, kPa) of live HAECs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-05-25 00:51:43"}

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Relative Metric

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