A DNA Barcode Library for North American Ephemeroptera: Progress and Prospects
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{"title"=>"A DNA barcode library for North American ephemeroptera: Progress and prospects", "type"=>"journal", "authors"=>[{"first_name"=>"Jeffrey M.", "last_name"=>"Webb", "scopus_author_id"=>"7403217303"}, {"first_name"=>"Luke M.", "last_name"=>"Jacobus", "scopus_author_id"=>"6603952911"}, {"first_name"=>"David H.", "last_name"=>"Funk", "scopus_author_id"=>"7103032341"}, {"first_name"=>"Xin", "last_name"=>"Zhou", "scopus_author_id"=>"55574214572"}, {"first_name"=>"Boris", "last_name"=>"Kondratieff", "scopus_author_id"=>"7003991200"}, {"first_name"=>"Christy J.", "last_name"=>"Geraci", "scopus_author_id"=>"15065329400"}, {"first_name"=>"R. Edward", "last_name"=>"DeWalt", "scopus_author_id"=>"6602825125"}, {"first_name"=>"Donald J.", "last_name"=>"Baird", "scopus_author_id"=>"7202707979"}, {"first_name"=>"Barton", "last_name"=>"Richard", "scopus_author_id"=>"7006993148"}, {"first_name"=>"Iain", "last_name"=>"Phillips", "scopus_author_id"=>"9133797900"}, {"first_name"=>"Paul D.N.", "last_name"=>"Hebert", "scopus_author_id"=>"7101661178"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84861589148", "sgr"=>"84861589148", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0038063", "pmid"=>"22666447", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pui"=>"364913246"}, "id"=>"e5cfa382-6ad5-30da-bda3-1a400a6f4f1f", "abstract"=>"DNA barcoding of aquatic macroinvertebrates holds much promise as a tool for taxonomic research and for providing the reliable identifications needed for water quality assessment programs. A prerequisite for identification using barcodes is a reliable reference library. We gathered 4165 sequences from the barcode region of the mitochondrial cytochrome c oxidase subunit I gene representing 264 nominal and 90 provisional species of mayflies (Insecta: Ephemeroptera) from Canada, Mexico, and the United States. No species shared barcode sequences and all can be identified with barcodes with the possible exception of some Caenis. Minimum interspecific distances ranged from 0.3-24.7% (mean: 12.5%), while the average intraspecific divergence was 1.97%. The latter value was inflated by the presence of very high divergences in some taxa. In fact, nearly 20% of the species included two or three haplotype clusters showing greater than 5.0% sequence divergence and some values are as high as 26.7%. Many of the species with high divergences are polyphyletic and likely represent species complexes. Indeed, many of these polyphyletic species have numerous synonyms and individuals in some barcode clusters show morphological attributes characteristic of the synonymized species. In light of our findings, it is imperative that type or topotype specimens be sequenced to correctly associate barcode clusters with morphological species concepts and to determine the status of currently synonymized species.", "link"=>"http://www.mendeley.com/research/dna-barcode-library-north-american-ephemeroptera-progress-prospects", "reader_count"=>83, "reader_count_by_academic_status"=>{"Unspecified"=>3, "Professor > Associate Professor"=>4, "Student > Doctoral Student"=>2, "Researcher"=>17, "Student > Ph. D. Student"=>18, "Student > Postgraduate"=>2, "Student > Master"=>18, "Other"=>4, "Student > Bachelor"=>10, "Lecturer > Senior Lecturer"=>2, "Professor"=>3}, "reader_count_by_user_role"=>{"Unspecified"=>3, "Professor > Associate Professor"=>4, "Student > Doctoral Student"=>2, "Researcher"=>17, "Student > Ph. D. Student"=>18, "Student > Postgraduate"=>2, "Student > Master"=>18, "Other"=>4, "Student > Bachelor"=>10, "Lecturer > Senior Lecturer"=>2, "Professor"=>3}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Environmental Science"=>9, "Biochemistry, Genetics and Molecular Biology"=>3, "Agricultural and Biological Sciences"=>65, "Medicine and Dentistry"=>3}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>65}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Unspecified"=>{"Unspecified"=>3}, "Environmental Science"=>{"Environmental Science"=>9}}, "reader_count_by_country"=>{"Canada"=>3, "United States"=>2, "Brazil"=>2, "Mexico"=>1, "France"=>1, "Germany"=>1, "Spain"=>1}, "group_count"=>2}

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Figshare

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  • {"files"=>["https://ndownloader.figshare.com/files/630532"], "description"=>"<p>Distribution map for all barcoded specimens of Ephemeroptera with geocoordinates (n = 3902).</p>", "links"=>[], "tags"=>["barcoded", "specimens", "ephemeroptera", "geocoordinates"], "article_id"=>301004, "categories"=>["Ecology", "Evolutionary Biology"], "users"=>["Jeffrey M. Webb", "Luke M. Jacobus", "David H. Funk", "Xin Zhou", "Boris Kondratieff", "Christy J. Geraci", "R. Edward DeWalt", "Donald J. Baird", "Barton Richard", "Iain Phillips", "Paul D. N. Hebert"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0038063.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Distribution_map_for_all_barcoded_specimens_of_Ephemeroptera_with_geocoordinates_n_8202_8202_3902_/301004", "title"=>"Distribution map for all barcoded specimens of Ephemeroptera with geocoordinates (n = 3902).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-05-30 00:16:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/630690"], "description"=>"<p>Diagnostic nucleotides in <i>Siphlonurus rapidus</i>, <i>S. typicus</i>, and <i>S</i>. sp.JMW1.</p>", "links"=>[], "tags"=>["nucleotides"], "article_id"=>301176, "categories"=>["Ecology", "Evolutionary Biology"], "users"=>["Jeffrey M. Webb", "Luke M. Jacobus", "David H. Funk", "Xin Zhou", "Boris Kondratieff", "Christy J. Geraci", "R. Edward DeWalt", "Donald J. Baird", "Barton Richard", "Iain Phillips", "Paul D. N. Hebert"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0038063.t002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Diagnostic_nucleotides_in_Siphlonurus_rapidus_S_typicus_and_S_sp_JMW1_/301176", "title"=>"Diagnostic nucleotides in <i>Siphlonurus rapidus</i>, <i>S. typicus</i>, and <i>S</i>. sp.JMW1.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-05-30 00:19:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/630746"], "description"=>"<p>All distances are % K2P; MNID = minimum interspecific K2P distance, MXID = maximum intraspecific K2P distance.</p>", "links"=>[], "tags"=>["barcode", "divergence", "21", "ephemeroptera", "america"], "article_id"=>301223, "categories"=>["Ecology", "Evolutionary Biology"], "users"=>["Jeffrey M. Webb", "Luke M. Jacobus", "David H. Funk", "Xin Zhou", "Boris Kondratieff", "Christy J. Geraci", "R. Edward DeWalt", "Donald J. Baird", "Barton Richard", "Iain Phillips", "Paul D. N. Hebert"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0038063.t001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Summary_of_current_barcode_coverage_and_divergence_values_for_the_21_families_of_Ephemeroptera_known_from_North_America_NA_/301223", "title"=>"Summary of current barcode coverage and divergence values for the 21 families of Ephemeroptera known from North America (NA).", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-05-30 00:20:23"}

PMC Usage Stats | Further Information

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Relative Metric

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