Metagenomics of the Svalbard Reindeer Rumen Microbiome Reveals Abundance of Polysaccharide Utilization Loci
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Metagenomics of the svalbard reindeer rumen microbiome reveals abundance of polysaccharide utilization loci", "type"=>"journal", "authors"=>[{"first_name"=>"Phillip B.", "last_name"=>"Pope", "scopus_author_id"=>"35076440900"}, {"first_name"=>"Alasdair K.", "last_name"=>"Mackenzie", "scopus_author_id"=>"56371227100"}, {"first_name"=>"Ivan", "last_name"=>"Gregor", "scopus_author_id"=>"55246928900"}, {"first_name"=>"Wendy", "last_name"=>"Smith", "scopus_author_id"=>"7406472618"}, {"first_name"=>"Monica A.", "last_name"=>"Sundset", "scopus_author_id"=>"6506647377"}, {"first_name"=>"Alice C.", "last_name"=>"McHardy", "scopus_author_id"=>"6602304583"}, {"first_name"=>"Mark", "last_name"=>"Morrison", "scopus_author_id"=>"7201675268"}, {"first_name"=>"Vincent G.H.", "last_name"=>"Eijsink", "scopus_author_id"=>"7006656581"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"364979238", "issn"=>"19326203", "isbn"=>"1932-6203", "doi"=>"10.1371/journal.pone.0038571", "scopus"=>"2-s2.0-84862001111", "pmid"=>"22701672", "sgr"=>"84862001111"}, "id"=>"74c14efb-7960-349d-a380-19375c3d1a44", "abstract"=>"Lignocellulosic biomass remains a largely untapped source of renewable energy predominantly due to its recalcitrance and an incomplete understanding of how this is overcome in nature. We present here a compositional and comparative analysis of metagenomic data pertaining to a natural biomass-converting ecosystem adapted to austere arctic nutritional conditions, namely the rumen microbiome of Svalbard reindeer (Rangifer tarandus platyrhynchus). Community analysis showed that deeply-branched cellulolytic lineages affiliated to the Bacteroidetes and Firmicutes are dominant, whilst sequence binning methods facilitated the assemblage of metagenomic sequence for a dominant and novel Bacteroidales clade (SRM-1). Analysis of unassembled metagenomic sequence as well as metabolic reconstruction of SRM-1 revealed the presence of multiple polysaccharide utilization loci-like systems (PULs) as well as members of more than 20 glycoside hydrolase and other carbohydrate-active enzyme families targeting various polysaccharides including cellulose, xylan and pectin. Functional screening of cloned metagenome fragments revealed high cellulolytic activity and an abundance of PULs that are rich in endoglucanases (GH5) but devoid of other common enzymes thought to be involved in cellulose degradation. Combining these results with known and partly re-evaluated metagenomic data strongly indicates that much like the human distal gut, the digestive system of herbivores harbours high numbers of deeply branched and as-yet uncultured members of the Bacteroidetes that depend on PUL-like systems for plant biomass degradation.", "link"=>"http://www.mendeley.com/research/metagenomics-svalbard-reindeer-rumen-microbiome-reveals-abundance-polysaccharide-utilization-loci", "reader_count"=>161, "reader_count_by_academic_status"=>{"Unspecified"=>3, "Professor > Associate Professor"=>3, "Researcher"=>39, "Student > Doctoral Student"=>12, "Student > Ph. D. Student"=>45, "Student > Postgraduate"=>10, "Student > Master"=>23, "Other"=>1, "Student > Bachelor"=>14, "Lecturer"=>1, "Professor"=>10}, "reader_count_by_user_role"=>{"Unspecified"=>3, "Professor > Associate Professor"=>3, "Researcher"=>39, "Student > Doctoral Student"=>12, "Student > Ph. D. Student"=>45, "Student > Postgraduate"=>10, "Student > Master"=>23, "Other"=>1, "Student > Bachelor"=>14, "Lecturer"=>1, "Professor"=>10}, "reader_count_by_subject_area"=>{"Unspecified"=>7, "Agricultural and Biological Sciences"=>113, "Veterinary Science and Veterinary Medicine"=>1, "Chemistry"=>3, "Computer Science"=>3, "Earth and Planetary Sciences"=>1, "Engineering"=>3, "Environmental Science"=>3, "Biochemistry, Genetics and Molecular Biology"=>19, "Mathematics"=>1, "Medicine and Dentistry"=>4, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Social Sciences"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Social Sciences"=>{"Social Sciences"=>1}, "Mathematics"=>{"Mathematics"=>1}, "Unspecified"=>{"Unspecified"=>7}, "Environmental Science"=>{"Environmental Science"=>3}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "Engineering"=>{"Engineering"=>3}, "Chemistry"=>{"Chemistry"=>3}, "Earth and Planetary Sciences"=>{"Earth and Planetary Sciences"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>113}, "Computer Science"=>{"Computer Science"=>3}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>19}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "reader_count_by_country"=>{"Sweden"=>1, "United States"=>2, "Philippines"=>1, "Brazil"=>5, "Denmark"=>1, "Italy"=>2, "United Kingdom"=>3, "Slovakia"=>1, "Kenya"=>1, "Germany"=>1, "India"=>3}, "group_count"=>7}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84862001111"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84862001111?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84862001111&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84862001111&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84862001111", "dc:identifier"=>"SCOPUS_ID:84862001111", "eid"=>"2-s2.0-84862001111", "dc:title"=>"Metagenomics of the svalbard reindeer rumen microbiome reveals abundance of polysaccharide utilization loci", "dc:creator"=>"Pope P.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"7", "prism:issueIdentifier"=>"6", "prism:pageRange"=>nil, "prism:coverDate"=>"2012-06-06", "prism:coverDisplayDate"=>"6 June 2012", "prism:doi"=>"10.1371/journal.pone.0038571", "citedby-count"=>"114", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Universitetet for miljø- og biovitenskap", "affiliation-city"=>"As", "affiliation-country"=>"Norway"}], "pubmed-id"=>"22701672", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e38571", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0038571", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"86", "xml_views"=>"1", "html_views"=>"380"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"38", "xml_views"=>"0", "html_views"=>"128"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"37", "xml_views"=>"5", "html_views"=>"85"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"42", "xml_views"=>"2", "html_views"=>"117"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"49", "xml_views"=>"0", "html_views"=>"146"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"42", "xml_views"=>"1", "html_views"=>"148"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"32", "xml_views"=>"0", "html_views"=>"129"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"42", "xml_views"=>"0", "html_views"=>"179"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"27", "xml_views"=>"2", "html_views"=>"150"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"126"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"130"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"130"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"3", "html_views"=>"130"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"26", "xml_views"=>"1", "html_views"=>"119"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"82"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"140"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"28", "xml_views"=>"2", "html_views"=>"321"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"28", "xml_views"=>"2", "html_views"=>"213"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"146"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"100"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"81"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"21", "xml_views"=>"2", "html_views"=>"131"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"135"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"129"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"108"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"101"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"3", "html_views"=>"132"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"25", "xml_views"=>"1", "html_views"=>"144"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"190"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"19", "xml_views"=>"2", "html_views"=>"155"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"14", "xml_views"=>"2", "html_views"=>"166"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"139"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"68"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"62"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"71"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"23", "xml_views"=>"1", "html_views"=>"85"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"101"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"86"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"103"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"108"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"88"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"85"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"71"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"110"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"92"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"52"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"18", "xml_views"=>"2", "html_views"=>"57"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"3", "html_views"=>"55"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"4", "html_views"=>"58"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"37"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"20", "xml_views"=>"2", "html_views"=>"57"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"66"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"65"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"37"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"2", "html_views"=>"50"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"3", "html_views"=>"34"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"28", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"28"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"22", "xml_views"=>"5", "html_views"=>"28"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"4", "html_views"=>"9"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"32", "xml_views"=>"2", "html_views"=>"31"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"26", "xml_views"=>"4", "html_views"=>"6"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"41", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"33", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"44", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"10", "year"=>"2020", "pdf_views"=>"22", "xml_views"=>"1", "html_views"=>"10"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/627828"], "description"=>"*<p>GHs are grouped according to their major functional role in the degradation of plant fiber. The numbers in parentheses represent the percentages of these groups relative to the total number of GH’s presented in this table. In addition to GHs, the table shows data for four other selected proteins possibly involved in biomass turnover; see main text for details. Note that some of the GH profiles were derived from contigs<sup>‡</sup> rather than (filtered) unassembled reads<sup>§</sup>, explaining part of the differences in absolute gene numbers. ¶ Data calculated in this study using searches against the rumen metagenome dataset <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038571#pone.0038571-Hess1\" target=\"_blank\">[7]</a> using SusC (PF00593) and SusD (PF07980) HMMs.<sup> †</sup>Pfam HMMs not available for CAZy families; data generated by dbCAN (<a href=\"http://csbl.bmb.uga.edu/dbCANdev/index.php\" target=\"_blank\">http://csbl.bmb.uga.edu/dbCANdev/index.php</a>).</p>", "links"=>[], "tags"=>["occurrence", "ghs", "targeting", "polysaccharides", "herbivore"], "article_id"=>298309, "categories"=>["Biological Sciences", "Ecology", "Genetics", "Microbiology"], "users"=>["Phillip B. Pope", "Alasdair K. Mackenzie", "Ivan Gregor", "Wendy Smith", "Monica A. Sundset", "Alice C. McHardy", "Mark Morrison", "Vincent G.H. Eijsink"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0038571.t001", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Overview_of_the_occurrence_of_GHs_targeting_plant_structural_polysaccharides_in_five_herbivore_metagenomes_/298309", "title"=>"Overview of the occurrence of GHs targeting plant structural polysaccharides in five herbivore metagenomes<sup>*</sup>.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-06-06 02:18:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/627780"], "description"=>"<p><b>Sb.</b> Substrate. <b>C</b> Cellulose degrading activity detected using carboxymethyl cellulose as a substrate. <b>X</b> Xylan degrading activity detected using birchwood xylan as a substrate. Carbohydrate binding modules (CBM or BACON) are indicated in superscript text. Note that Sc00001 and Sc00021 do not contain any gene(s) coding for proteins known to be involved in the degradation of cellulose. Partial scaffolds not encoding documented cellulose active genes are excluded (n = 13).</p>", "links"=>[], "tags"=>["glycoside", "hydrolases", "sus", "proteins", "encoded", "scaffolds", "reconstructed", "sequenced", "fosmids", "genomic"], "article_id"=>298254, "categories"=>["Biological Sciences", "Ecology", "Genetics", "Microbiology"], "users"=>["Phillip B. Pope", "Alasdair K. Mackenzie", "Ivan Gregor", "Wendy Smith", "Monica A. Sundset", "Alice C. McHardy", "Mark Morrison", "Vincent G.H. Eijsink"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0038571.t002", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Summary_of_glycoside_hydrolases_and_Sus_proteins_encoded_within_scaffolds_reconstructed_from_sequenced_fosmids_selected_from_functional_genomic_screens_/298254", "title"=>"Summary of glycoside hydrolases and Sus proteins encoded within scaffolds reconstructed from sequenced fosmids selected from functional genomic screens.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-06-06 02:17:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/325277", "https://ndownloader.figshare.com/files/325500", "https://ndownloader.figshare.com/files/325547", "https://ndownloader.figshare.com/files/325614", "https://ndownloader.figshare.com/files/325689", "https://ndownloader.figshare.com/files/325741"], "description"=>"<div><p>Lignocellulosic biomass remains a largely untapped source of renewable energy predominantly due to its recalcitrance and an incomplete understanding of how this is overcome in nature. We present here a compositional and comparative analysis of metagenomic data pertaining to a natural biomass-converting ecosystem adapted to austere arctic nutritional conditions, namely the rumen microbiome of Svalbard reindeer (<em>Rangifer tarandus platyrhynchus</em>). Community analysis showed that deeply-branched cellulolytic lineages affiliated to the Bacteroidetes and Firmicutes are dominant, whilst sequence binning methods facilitated the assemblage of metagenomic sequence for a dominant and novel Bacteroidales clade (SRM-1). Analysis of unassembled metagenomic sequence as well as metabolic reconstruction of SRM-1 revealed the presence of multiple polysaccharide utilization loci-like systems (PULs) as well as members of more than 20 glycoside hydrolase and other carbohydrate-active enzyme families targeting various polysaccharides including cellulose, xylan and pectin. Functional screening of cloned metagenome fragments revealed high cellulolytic activity and an abundance of PULs that are rich in endoglucanases (GH5) but devoid of other common enzymes thought to be involved in cellulose degradation. Combining these results with known and partly re-evaluated metagenomic data strongly indicates that much like the human distal gut, the digestive system of herbivores harbours high numbers of deeply branched and as-yet uncultured members of the Bacteroidetes that depend on PUL-like systems for plant biomass degradation.</p> </div>", "links"=>[], "tags"=>["metagenomics", "svalbard", "reindeer", "rumen", "microbiome", "reveals", "abundance", "polysaccharide", "utilization", "loci"], "article_id"=>124211, "categories"=>["Biological Sciences", "Ecology", "Genetics", "Microbiology"], "users"=>["Phillip B. Pope", "Alasdair K. Mackenzie", "Ivan Gregor", "Wendy Smith", "Monica A. Sundset", "Alice C. McHardy", "Mark Morrison", "Vincent G.H. Eijsink"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0038571.s001", "https://dx.doi.org/10.1371/journal.pone.0038571.s002", "https://dx.doi.org/10.1371/journal.pone.0038571.s003", "https://dx.doi.org/10.1371/journal.pone.0038571.s004", "https://dx.doi.org/10.1371/journal.pone.0038571.s005", "https://dx.doi.org/10.1371/journal.pone.0038571.s006"], "stats"=>{"downloads"=>17, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Metagenomics_of_the_Svalbard_Reindeer_Rumen_Microbiome_Reveals_Abundance_of_Polysaccharide_Utilization_Loci/124211", "title"=>"Metagenomics of the Svalbard Reindeer Rumen Microbiome Reveals Abundance of Polysaccharide Utilization Loci", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-06-06 01:10:11"}
  • {"files"=>["https://ndownloader.figshare.com/files/627701"], "description"=>"<p>Gene clusters were recovered from the cow rumen metagenome (partial genome of an as-yet uncultured Bacteroidales phylotype AC2a) and sequenced cellulolytic fosmids constructed from environmental DNA originating from the gut microbiomes of the wallaby foregut and the reindeer and buffalo rumen (upper four clusters). All fosmid sequences originate from Bacteroidetes, according to PhyloPythiaS binning, and for all cellulolytic activity has been detected in functional screens. Green genes represent SusE/SusF-like genes predicted to encode outer-membrane proteins whose function is currently unknown. Black genes encode putative response-regulators. BACON: indicates a carbohydrate binding domain identified by <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038571#pone.0038571-Mello1\" target=\"_blank\">[40]</a>. TonB: indicates members of the TonB-dependent receptor family, a group of outer membrane spanning β-barrel proteins that transport solutes and macromolecules. TW-64 and TW-33 correspond to sample IDs for the GH9 and GH5 genes (respectively) encoded within the AC2a PUL, from which expressed proteins were tested positive for hydrolytic activity on various cellulosic substrates by Hess et al., <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038571#pone.0038571-Hess1\" target=\"_blank\">[7]</a>. GenBank accession numbers and/or IMG Gene Object ID numbers are provided. Gene IDs for the AC2a PUL are provided in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038571#pone.0038571.s005\" target=\"_blank\">Table S5</a>.</p>", "links"=>[], "tags"=>["presumptive", "cellulolytic", "puls", "svalbard", "reindeer", "rumen"], "article_id"=>298178, "categories"=>["Biological Sciences", "Ecology", "Genetics", "Microbiology"], "users"=>["Phillip B. Pope", "Alasdair K. Mackenzie", "Ivan Gregor", "Wendy Smith", "Monica A. Sundset", "Alice C. McHardy", "Mark Morrison", "Vincent G.H. Eijsink"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0038571.g002", "stats"=>{"downloads"=>3, "page_views"=>23, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparative_gene_organization_of_presumptive_cellulolytic_PULs_identified_in_the_Svalbard_reindeer_rumen_and_other_gut_environments_/298178", "title"=>"Comparative gene organization of presumptive cellulolytic PULs identified in the Svalbard reindeer rumen and other gut environments.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-06-06 02:16:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/627580"], "description"=>"<p>(<b>A</b>) Diversity and relative abundance of the most abundant bacterial taxa identified in the rumen of the Svalbard reindeer based on phylogenetic analysis of 16S rRNA genes. (<b>B</b>) OTU network map showing OTU interactions between all rarefied samples from the Svalbard reindeer, Norwegian reindeer feeding on a commercial feed, Tammar wallaby, rumen and termite. OTUs are represented by dots and dot sizes reflect sequence counts within the OTU. Dot colour indicates the number of microbiomes in which the OTU was found (1 =  white, 2 =  yellow, 3 =  orange, 4 =  red). The lines radiating from each of the five grey dots link the OTUs to their source microbiomes: Svalbard reindeer, dark blue (this study); Norwegian reindeer, light blue (this study; dataset included for comparative purposes only); Termite_PL3, yellow <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038571#pone.0038571-Warnecke1\" target=\"_blank\">[4]</a>; bovine, green <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038571#pone.0038571-Brulc1\" target=\"_blank\">[6]</a>; Tammar wallaby, orange <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038571#pone.0038571-Pope1\" target=\"_blank\">[5]</a>. (<b>C</b>) Principal coordinate axes (PCoA) for the unweighted UniFrac analyses are coloured by host animal; Svalbard reindeer (2 samples: ▸▴) dark-blue; Norwegian reindeer (2 samples: ♦♦) light-blue; bovine rumen (•) green; Tammar wallaby (◂) red; termite (▪) orange. (<b>D</b>) Composition of the Svalbard reindeer rumen metagenome sequence dataset, based on sequence composition-based binning of 1394 assembled scaffolds (∼5.4 Mb) using PhyloPythiaS. For a complete <i>rrs</i> inventory and comparisons between the two Svalbard reindeer samples at an OTU definition of 97% ID see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038571#pone.0038571.s001\" target=\"_blank\">Table S1</a>.</p>", "links"=>[], "tags"=>["svalbard", "reindeer", "rumen", "microbiome", "microbiomes"], "article_id"=>298059, "categories"=>["Biological Sciences", "Ecology", "Genetics", "Microbiology"], "users"=>["Phillip B. Pope", "Alasdair K. Mackenzie", "Ivan Gregor", "Wendy Smith", "Monica A. Sundset", "Alice C. McHardy", "Mark Morrison", "Vincent G.H. Eijsink"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0038571.g001", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Microbial_community_analysis_of_the_Svalbard_reindeer_rumen_microbiome_and_comparison_with_microbiomes_from_other_selected_gut_environments_/298059", "title"=>"Microbial community analysis of the Svalbard reindeer rumen microbiome and comparison with microbiomes from other selected gut environments.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-06-06 02:14:19"}

PMC Usage Stats | Further Information

  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"25", "year"=>"2012", "pdf"=>"18", "unique-ip"=>"26", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"31", "full-text"=>"27", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"27", "full-text"=>"26", "pdf"=>"15", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"26", "full-text"=>"22", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"22", "full-text"=>"20", "pdf"=>"11", "abstract"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"23", "full-text"=>"23", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"18", "full-text"=>"18", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"19", "full-text"=>"16", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"31", "full-text"=>"27", "pdf"=>"17", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"23", "full-text"=>"21", "pdf"=>"10", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"19", "full-text"=>"13", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"18", "full-text"=>"26", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"17", "full-text"=>"22", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"26", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"15", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"29", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"22", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"18", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"17", "full-text"=>"13", "pdf"=>"6", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"12", "full-text"=>"57", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"17", "full-text"=>"63", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"17", "full-text"=>"61", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"15", "full-text"=>"81", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"27", "full-text"=>"154", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"9", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"18", "full-text"=>"25", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"28", "full-text"=>"34", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"37", "full-text"=>"53", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"18", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"24", "full-text"=>"34", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"17", "full-text"=>"23", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"23", "full-text"=>"103", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"18", "full-text"=>"19", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"21", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"18", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"18", "full-text"=>"18", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"17", "full-text"=>"13", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"4", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"14", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"8", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"17", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"17", "full-text"=>"15", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"15", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"9"}

Relative Metric

{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Ecology", "average_usage"=>[350, 552, 657, 754, 839, 932, 1023, 1103, 1197, 1274, 1359, 1436, 1499, 1564, 1640, 1726, 1794, 1865, 1933, 2021, 2101, 2181, 2239, 2324, 2376]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[333, 576, 707, 814, 908, 1004, 1104, 1197, 1280, 1370, 1449, 1531, 1603, 1673, 1742, 1817, 1886, 1954, 2025, 2098, 2171, 2234, 2304, 2365, 2431]}, {"subject_area"=>"/Biology and life sciences/Microbiology", "average_usage"=>[339, 580, 709, 814, 907, 1003, 1095, 1190, 1286, 1375, 1466, 1546, 1622, 1694, 1765, 1830, 1902, 1978, 2054, 2122, 2194, 2269, 2330, 2388, 2460]}, {"subject_area"=>"/Biology and life sciences/Plant science", "average_usage"=>[329, 543, 667, 773, 865, 963, 1066, 1149, 1233, 1323, 1411, 1500, 1588, 1661, 1732, 1803, 1887, 1978, 2057, 2138, 2203, 2283, 2363, 2419, 2493]}, {"subject_area"=>"/Ecology and environmental sciences/Ecology", "average_usage"=>[347, 547, 641, 742, 836, 927, 1016, 1099, 1184, 1267, 1348, 1418, 1484, 1555, 1631, 1705, 1788, 1843, 1917, 1985, 2052, 2115, 2190, 2258, 2333]}, {"subject_area"=>"/Ecology and environmental sciences/Plant ecology", "average_usage"=>[268, 431, 534, 622, 723, 812, 887, 945, 1036, 1094, 1183, 1264, 1302]}, {"subject_area"=>"/Physical sciences", "average_usage"=>[304, 506, 616, 712, 799, 879, 968, 1052, 1134, 1212, 1284, 1357, 1427, 1494, 1557, 1621, 1689, 1756, 1823, 1883, 1944, 1997, 2056, 2118, 2171]}]}
Loading … Spinner
There are currently no alerts