Barcoding Sponges: An Overview Based on Comprehensive Sampling
Publication Date
July 03, 2012
Journal
PLOS ONE
Authors
Sergio Vargas, Astrid Schuster, Katharina Sacher, Gabrielle Büttner, et al
Volume
7
Issue
7
Pages
e39345
DOI
https://dx.plos.org/10.1371/journal.pone.0039345
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0039345
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/22802937
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3389008
Europe PMC
http://europepmc.org/abstract/MED/22802937
Web of Science
000306186900011
Scopus
84863610010
Mendeley
http://www.mendeley.com/research/barcoding-sponges-overview-based-comprehensive-sampling
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Barcoding sponges: An overview based on comprehensive sampling", "type"=>"journal", "authors"=>[{"first_name"=>"Sergio", "last_name"=>"Vargas", "scopus_author_id"=>"23399203200"}, {"first_name"=>"Astrid", "last_name"=>"Schuster", "scopus_author_id"=>"55027068100"}, {"first_name"=>"Katharina", "last_name"=>"Sacher", "scopus_author_id"=>"36449583900"}, {"first_name"=>"Gabrielle", "last_name"=>"Büttner", "scopus_author_id"=>"55398858400"}, {"first_name"=>"Simone", "last_name"=>"Schätzle", "scopus_author_id"=>"55026110800"}, {"first_name"=>"Benjamin", "last_name"=>"Läuchli", "scopus_author_id"=>"37047359500"}, {"first_name"=>"Kathryn", "last_name"=>"Hall", "scopus_author_id"=>"7402946415"}, {"first_name"=>"John N.A.", "last_name"=>"Hooper", "scopus_author_id"=>"24503051000"}, {"first_name"=>"Dirk", "last_name"=>"Erpenbeck", "scopus_author_id"=>"14035364200"}, {"first_name"=>"Gert", "last_name"=>"Wörheide", "scopus_author_id"=>"6602867063"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84863610010", "pui"=>"365201702", "doi"=>"10.1371/journal.pone.0039345", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "sgr"=>"84863610010", "pmid"=>"22802937"}, "id"=>"48849c5f-9a4d-3d85-a3bf-8814ecc152a3", "abstract"=>"BACKGROUND: Phylum Porifera includes ∼8,500 valid species distributed world-wide in aquatic ecosystems ranging from ephemeral fresh-water bodies to coastal environments and the deep-sea. The taxonomy and systematics of sponges is complicated, and morphological identification can be both time consuming and erroneous due to phenotypic convergence and secondary losses, etc. DNA barcoding can provide sponge biologists with a simple and rapid method for the identification of samples of unknown taxonomic membership. The Sponge Barcoding Project (www.spongebarcoding.org), the first initiative to barcode a non-bilaterian metazoan phylum, aims to provide a comprehensive DNA barcode database for Phylum Porifera.\\n\\nMETHODOLOGY/PRINCIPAL FINDINGS: ∼7,400 sponge specimens have been extracted, and amplification of the standard COI barcoding fragment has been attempted for approximately 3,300 museum samples with ∼25% mean amplification success. Based on this comprehensive sampling, we present the first report on the workflow and progress of the sponge barcoding project, and discuss some common pitfalls inherent to the barcoding of sponges.\\n\\nCONCLUSION: A DNA-barcoding workflow capable of processing potentially large sponge collections has been developed and is routinely used for the Sponge Barcoding Project with success. Sponge specific problems such as the frequent co-amplification of non-target organisms have been detected and potential solutions are currently under development. The initial success of this innovative project have already demonstrated considerable refinement of sponge systematics, evaluating morphometric character importance, geographic phenotypic variability, and the utility of the standard barcoding fragment for Porifera (despite its conserved evolution within this basal metazoan phylum).", "link"=>"http://www.mendeley.com/research/barcoding-sponges-overview-based-comprehensive-sampling", "reader_count"=>137, "reader_count_by_academic_status"=>{"Unspecified"=>6, "Professor > Associate Professor"=>9, "Librarian"=>1, "Researcher"=>24, "Student > Doctoral Student"=>5, "Student > Ph. D. Student"=>37, "Student > Postgraduate"=>4, "Student > Master"=>28, "Other"=>3, "Student > Bachelor"=>12, "Lecturer"=>2, "Professor"=>6}, "reader_count_by_user_role"=>{"Unspecified"=>6, "Professor > Associate Professor"=>9, "Librarian"=>1, "Researcher"=>24, "Student > Doctoral Student"=>5, "Student > Ph. D. Student"=>37, "Student > Postgraduate"=>4, "Student > Master"=>28, "Other"=>3, "Student > Bachelor"=>12, "Lecturer"=>2, "Professor"=>6}, "reader_count_by_subject_area"=>{"Unspecified"=>7, "Environmental Science"=>8, "Biochemistry, Genetics and Molecular Biology"=>11, "Agricultural and Biological Sciences"=>93, "Medicine and Dentistry"=>2, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Chemistry"=>5, "Computer Science"=>4, "Immunology and Microbiology"=>2, "Earth and Planetary Sciences"=>4}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Chemistry"=>{"Chemistry"=>5}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Earth and Planetary Sciences"=>{"Earth and Planetary Sciences"=>4}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>93}, "Computer Science"=>{"Computer Science"=>4}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>11}, "Unspecified"=>{"Unspecified"=>7}, "Environmental Science"=>{"Environmental Science"=>8}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"Netherlands"=>1, "United States"=>4, "Brazil"=>2, "Mexico"=>3, "Israel"=>1, "Australia"=>1, "Portugal"=>2, "Germany"=>2, "Spain"=>1}, "group_count"=>2}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84863610010"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84863610010?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84863610010&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84863610010&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84863610010", "dc:identifier"=>"SCOPUS_ID:84863610010", "eid"=>"2-s2.0-84863610010", "dc:title"=>"Barcoding sponges: An overview based on comprehensive sampling", "dc:creator"=>"Vargas S.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"7", "prism:issueIdentifier"=>"7", "prism:pageRange"=>nil, "prism:coverDate"=>"2012-07-03", "prism:coverDisplayDate"=>"3 July 2012", "prism:doi"=>"10.1371/journal.pone.0039345", "citedby-count"=>"43", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Ludwig-Maximilians-Universität München", "affiliation-city"=>"Munich", "affiliation-country"=>"Germany"}], "pubmed-id"=>"22802937", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e39345", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0039345", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Twitter

Counter

  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"338", "xml_views"=>"9", "html_views"=>"1434"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"47", "xml_views"=>"7", "html_views"=>"140"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"28", "xml_views"=>"0", "html_views"=>"120"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"123"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"32", "xml_views"=>"1", "html_views"=>"123"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"75"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"84"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"16", "xml_views"=>"2", "html_views"=>"80"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"87"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"18", "xml_views"=>"2", "html_views"=>"88"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"87"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"59"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"80"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"70"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"86"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"14", "xml_views"=>"2", "html_views"=>"84"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"86"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"51"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"54"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"69"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"91"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"3", "html_views"=>"56"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"79"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"101"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"67"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"58"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"81"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"98"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"18", "xml_views"=>"2", "html_views"=>"87"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"70"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"75"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"63"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"96"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"92"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"91"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"83"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"74"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"113"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"89"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"70"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"63"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"86"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"69"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"54"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"90"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"4", "html_views"=>"80"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"15", "xml_views"=>"2", "html_views"=>"142"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"53"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"74"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"84"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"67"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"82"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"35", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"36", "xml_views"=>"2", "html_views"=>"55"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"40"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"19", "xml_views"=>"4", "html_views"=>"32"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"23", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"29", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"36", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"21", "xml_views"=>"4", "html_views"=>"47"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"24", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"45", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"48", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"34", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"4"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/614602"], "description"=>"<p>Grey and black colours represent failed and positive reactions, respectively. Only families with more than 30 documented PCRs are included in the figure, these taxa correspond to the families analised in the generalised linear model. Asterisks on the right side correspond to the different family groups analysed: *: Acarnidae, Ancorinidae, Axinellidae, Dictyonellidae, Dysideidae, Halichondridae, Iotrochotidae, Microcionidae, Mycalidae, Plakinidae, Raspailidae, Tedaniidae, Tetillidae and Thorectidae; **: Chalinidae, Clionaidae and Suberitidae; ***: Chondropsidae, Coelosphaeridae, Crellidae, Desmacellidae, Isodictyidae and Podospongiidae.</p>", "links"=>[], "tags"=>["barcoding", "partition", "sponge"], "article_id"=>285088, "categories"=>["Molecular Biology", "Inorganic Chemistry", "Biochemistry", "Ecology", "Evolutionary Biology"], "users"=>["Sergio Vargas", "Astrid Schuster", "Katharina Sacher", "Gabrielle Büttner", "Simone Schätzle", "Benjamin Läuchli", "Kathryn Hall", "John N. A. Hooper", "Dirk Erpenbeck", "Gert Wörheide"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0039345.g001", "stats"=>{"downloads"=>4, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Amplification_success_of_the_standard_barcoding_COI_partition_per_sponge_family_/285088", "title"=>"Amplification success of the standard barcoding <i>COI</i> partition per sponge family.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-07-03 01:24:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/614728"], "description"=>"<p>Grey and black colours represent sequences corresponding to non-target organisms and poriferans, respectively. The included families correspond to the families used for the analysis of PCR success, and were analised in the generalised linear model. Asterisks on the right side correspond to the different family groups analysed: *: Acarnidae, Ancorinidae, Axinellidae, Dictyonellidae, Dysideidae, Halichondridae, Iotrochotidae, Microcionidae, Mycalidae, Plakinidae, Raspailidae, Tedaniidae, Tetillidae and Thorectidae; **: Chalinidae, Clionaidae and Suberitidae; ***: Chondropsidae, Coelosphaeridae, Crellidae, Desmacellidae, Isodictyidae and Podospongiidae.</p>", "links"=>[], "tags"=>["rates", "sponge"], "article_id"=>285216, "categories"=>["Molecular Biology", "Inorganic Chemistry", "Biochemistry", "Ecology", "Evolutionary Biology"], "users"=>["Sergio Vargas", "Astrid Schuster", "Katharina Sacher", "Gabrielle Büttner", "Simone Schätzle", "Benjamin Läuchli", "Kathryn Hall", "John N. A. Hooper", "Dirk Erpenbeck", "Gert Wörheide"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0039345.g002", "stats"=>{"downloads"=>0, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sequencing_success_rates_per_sponge_family_/285216", "title"=>"Sequencing success rates per sponge family.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-07-03 01:26:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/320280", "https://ndownloader.figshare.com/files/320364"], "description"=>"<div><h3>Background</h3><p>Phylum Porifera includes ∼8,500 valid species distributed world-wide in aquatic ecosystems ranging from ephemeral fresh-water bodies to coastal environments and the deep-sea. The taxonomy and systematics of sponges is complicated, and morphological identification can be both time consuming and erroneous due to phenotypic convergence and secondary losses, etc. DNA barcoding can provide sponge biologists with a simple and rapid method for the identification of samples of unknown taxonomic membership. The Sponge Barcoding Project (<a href=\"http://www.spongebarcoding.org\">www.spongebarcoding.org</a>), the first initiative to barcode a non-bilaterian metazoan phylum, aims to provide a comprehensive DNA barcode database for Phylum Porifera.</p> <h3>Methodology/Principal Findings</h3><p>∼7,400 sponge specimens have been extracted, and amplification of the standard COI barcoding fragment has been attempted for approximately 3,300 museum samples with ∼25% mean amplification success. Based on this comprehensive sampling, we present the first report on the workflow and progress of the sponge barcoding project, and discuss some common pitfalls inherent to the barcoding of sponges.</p> <h3>Conclusion</h3><p>A DNA-barcoding workflow capable of processing potentially large sponge collections has been developed and is routinely used for the Sponge Barcoding Project with success. Sponge specific problems such as the frequent co-amplification of non-target organisms have been detected and potential solutions are currently under development. The initial success of this innovative project have already demonstrated considerable refinement of sponge systematics, evaluating morphometric character importance, geographic phenotypic variability, and the utility of the standard barcoding fragment for Porifera (despite its conserved evolution within this basal metazoan phylum).</p> </div>", "links"=>[], "tags"=>["barcoding", "overview", "based", "sampling"], "article_id"=>123221, "categories"=>["Molecular Biology", "Inorganic Chemistry", "Biochemistry", "Ecology", "Evolutionary Biology"], "users"=>["Sergio Vargas", "Astrid Schuster", "Katharina Sacher", "Gabrielle Büttner", "Simone Schätzle", "Benjamin Läuchli", "Kathryn Hall", "John N. A. Hooper", "Dirk Erpenbeck", "Gert Wörheide"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0039345.s001", "https://dx.doi.org/10.1371/journal.pone.0039345.s002"], "stats"=>{"downloads"=>2, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Barcoding_Sponges_An_Overview_Based_on_Comprehensive_Sampling/123221", "title"=>"Barcoding Sponges: An Overview Based on Comprehensive Sampling", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-07-03 00:53:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/614862"], "description"=>"<p>The reported values, are for the core family group (Acarnidae, Ancorinidae, Axinellidae, Dyctionellidae, Dysideidae, Halichondridae, Iotrochotidae, Microcionidae, Mycalidae, Plakinidae, Raspailidae, Tedaniidae, Tetillidae and Thorectidae; upper value) and for this group together with the families Chalinidae, Clionaidae and Suberitidae (middle value), and with the families Chondropsidae, Coelosphaeridae, Crellidae, Desmacellidae, Isodictyidae and Podospongiidae (lower value). N.S. = not significant.</p>", "links"=>[], "tags"=>["linear", "logit", "taxonomic", "affiliation", "sequencing"], "article_id"=>285347, "categories"=>["Molecular Biology", "Inorganic Chemistry", "Biochemistry", "Ecology", "Evolutionary Biology"], "users"=>["Sergio Vargas", "Astrid Schuster", "Katharina Sacher", "Gabrielle Büttner", "Simone Schätzle", "Benjamin Läuchli", "Kathryn Hall", "John N. A. Hooper", "Dirk Erpenbeck", "Gert Wörheide"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0039345.t002", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Generalised_linear_model_binomial_errors_logit_link_of_the_effect_of_sample_taxonomic_affiliation_and_sample_age_over_sequencing_success_/285347", "title"=>"Generalised linear model (binomial errors, logit link) of the effect of sample taxonomic affiliation and sample age over sequencing success.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-07-03 01:29:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/614897"], "description"=>"1<p>Lysis mix: 100 mM NaCl, 50 mM Tris-HCl pH 8.0, 10 mM EDTA pH 8.0, 0.5% SDS, Proteinase K 10% v/v.</p>2<p>Binding buffer: 6 M GuSCN, 20 mM EDTA pH 8.0, 10 mM Tris-HCl pH 6.4, Triton X-100 4% v/v. The Binding mix is a 50% v/v solution of Binding Buffer in ethanol 96%.</p>3<p>Protein wash buffer is a 30% v/v solution of Binding Buffer in ethanol 96%.</p>4<p>Wash buffer: 50 mM NaCl, 10 mM Tris-HCl pH 7.4, 0.5 mM EDTA pH 8.0, ethanol 60%.</p>", "links"=>[], "tags"=>["genomic", "dna", "ivanova", "et", "2006", "sponge"], "article_id"=>285381, "categories"=>["Molecular Biology", "Inorganic Chemistry", "Biochemistry", "Ecology", "Evolutionary Biology"], "users"=>["Sergio Vargas", "Astrid Schuster", "Katharina Sacher", "Gabrielle Büttner", "Simone Schätzle", "Benjamin Läuchli", "Kathryn Hall", "John N. A. Hooper", "Dirk Erpenbeck", "Gert Wörheide"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0039345.t003", "stats"=>{"downloads"=>4, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Modifications_to_the_genomic_DNA_protocol_of_Ivanova_et_al_2006_used_for_sponge_barcoding_/285381", "title"=>"Modifications to the genomic DNA protocol of Ivanova et al. 2006 used for sponge barcoding.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-07-03 01:29:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/614941"], "description"=>"<p>The reported values, are for the core family group (Acarnidae, Ancorinidae, Axinellidae, Dyctionellidae, Dysideidae, Halichondridae, Iotrochotidae, Microcionidae, Mycalidae, Plakinidae, Raspailidae, Tedaniidae, Tetillidae and Thorectidae; upper value) and for this group together with the families Chalinidae, Clionaidae and Suberitidae (middle value), and with the families Chondropsidae, Coelosphaeridae, Crellidae, Desmacellidae, Isodictyidae and Podospongiidae (lower value).</p>", "links"=>[], "tags"=>["linear", "logit", "taxonomic", "affiliation", "pcr"], "article_id"=>285426, "categories"=>["Molecular Biology", "Inorganic Chemistry", "Biochemistry", "Ecology", "Evolutionary Biology"], "users"=>["Sergio Vargas", "Astrid Schuster", "Katharina Sacher", "Gabrielle Büttner", "Simone Schätzle", "Benjamin Läuchli", "Kathryn Hall", "John N. A. Hooper", "Dirk Erpenbeck", "Gert Wörheide"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0039345.t001", "stats"=>{"downloads"=>3, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Generalised_linear_model_binomial_errors_logit_link_of_the_effect_of_sample_taxonomic_affiliation_and_sample_age_over_PCR_success_/285426", "title"=>"Generalised linear model (binomial errors, logit link) of the effect of sample taxonomic affiliation and sample age over PCR success.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-07-03 01:30:26"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"19", "full-text"=>"23", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"17", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"10", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"6", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"16", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"27", "full-text"=>"31", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"16", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"22", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"2", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"18", "full-text"=>"34", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"10", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"18", "full-text"=>"17", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"17", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"20", "full-text"=>"20", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"36", "full-text"=>"32", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"18", "full-text"=>"20", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"15", "full-text"=>"18", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"24", "full-text"=>"25", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"17", "full-text"=>"20", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"22", "full-text"=>"31", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"24", "full-text"=>"32", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"23", "full-text"=>"16", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"22", "full-text"=>"23", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"21", "full-text"=>"28", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"23", "full-text"=>"30", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"29", "full-text"=>"29", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"17", "full-text"=>"20", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"28", "full-text"=>"28", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"20", "full-text"=>"27", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"23", "full-text"=>"21", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"21", "full-text"=>"21", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"31", "full-text"=>"33", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"17", "full-text"=>"19", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"24", "full-text"=>"26", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"18", "full-text"=>"22", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"25", "full-text"=>"32", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"27", "full-text"=>"38", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"39", "full-text"=>"46", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"29", "full-text"=>"38", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"29", "full-text"=>"38", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"41", "full-text"=>"48", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"35", "full-text"=>"40", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"40", "full-text"=>"50", "pdf"=>"9", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"36", "full-text"=>"48", "pdf"=>"9", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"22", "full-text"=>"30", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"22", "full-text"=>"28", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"28", "full-text"=>"35", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}

Relative Metric

{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[322, 550, 671, 773, 864, 955, 1048, 1135, 1223, 1308, 1387, 1465, 1534, 1602, 1673, 1744, 1813, 1885, 1955, 2026, 2093, 2160, 2228, 2290, 2349]}, {"subject_area"=>"/Biology and life sciences/Evolutionary biology", "average_usage"=>[366, 574, 695, 795, 885, 973, 1063, 1148, 1233, 1319, 1389, 1459, 1533, 1611, 1685, 1754, 1820, 1882, 1937, 2001, 2071, 2129, 2219, 2273, 2335]}, {"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[324, 551, 679, 782, 879, 971, 1064, 1154, 1245, 1328, 1406, 1476, 1541, 1617, 1683, 1754, 1818, 1893, 1962, 2025, 2092, 2164, 2227, 2294, 2355]}, {"subject_area"=>"/Computer and information sciences", "average_usage"=>[352, 587, 696, 809, 901, 989, 1072, 1156, 1257, 1334, 1422, 1486, 1555, 1647, 1714, 1780, 1844, 1919, 1997, 2051, 2138, 2198, 2267, 2324, 2391]}, {"subject_area"=>"/Physical sciences", "average_usage"=>[304, 506, 616, 712, 799, 879, 968, 1052, 1134, 1212, 1284, 1357, 1427, 1494, 1557, 1621, 1689, 1756, 1823, 1883, 1944, 1997, 2056, 2118, 2171]}, {"subject_area"=>"/Physical sciences/Mathematics", "average_usage"=>[325, 522, 627, 718, 804, 884, 969, 1052, 1131, 1207, 1277, 1346, 1415, 1478, 1542, 1605, 1663, 1723, 1776, 1839, 1895, 1955, 2008, 2066, 2123]}]}
Loading … Spinner
There are currently no alerts