Effects of fou8/fry1 Mutation on Sulfur Metabolism: Is Decreased Internal Sulfate the Trigger of Sulfate Starvation Response?
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{"title"=>"Effects of fou8/fry1 mutation on sulfur metabolism: Is decreased internal sulfate the trigger of sulfate starvation response?", "type"=>"journal", "authors"=>[{"first_name"=>"Bok Rye", "last_name"=>"Lee", "scopus_author_id"=>"7405438761"}, {"first_name"=>"Stine", "last_name"=>"Huseby", "scopus_author_id"=>"55255634200"}, {"first_name"=>"Anna", "last_name"=>"Koprivova", "scopus_author_id"=>"6603124252"}, {"first_name"=>"Aurore", "last_name"=>"Chételat", "scopus_author_id"=>"6603116066"}, {"first_name"=>"Markus", "last_name"=>"Wirtz", "scopus_author_id"=>"35516994200"}, {"first_name"=>"Sam T.", "last_name"=>"Mugford", "scopus_author_id"=>"12770357800"}, {"first_name"=>"Emily", "last_name"=>"Navid", "scopus_author_id"=>"55255708300"}, {"first_name"=>"Charles", "last_name"=>"Brearley", "scopus_author_id"=>"7004325474"}, {"first_name"=>"Shikha", "last_name"=>"Saha", "scopus_author_id"=>"23393629400"}, {"first_name"=>"Richard", "last_name"=>"Mithen", "scopus_author_id"=>"57195761987"}, {"first_name"=>"Rüdiger", "last_name"=>"Hell", "scopus_author_id"=>"6603859288"}, {"first_name"=>"Edward E.", "last_name"=>"Farmer", "scopus_author_id"=>"7102557538"}, {"first_name"=>"Stanislav", "last_name"=>"Kopriva", "scopus_author_id"=>"7003739536"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"22724014", "doi"=>"10.1371/journal.pone.0039425", "sgr"=>"84862490490", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "scopus"=>"2-s2.0-84862490490", "issn"=>"19326203", "pui"=>"365024046"}, "id"=>"34ee98d6-9435-3348-95b9-d891d6fcec07", "abstract"=>"The fou8 loss of function allele of adenosine bisphosphate phosphatase FIERY1 results in numerous phenotypes including the increased enzymatic oxygenation of fatty acids and increased jasmonate synthesis. Here we show that the mutation causes also profound alterations of sulfur metabolism. The fou8 mutants possess lower levels of sulfated secondary compounds, glucosinolates, and accumulate the desulfo-precursors similar to previously described mutants in adenosine 5'phosphosulfate kinase. Transcript levels of genes involved in sulfate assimilation differ in fou8 compared to wild type Col-0 plants and are similar to plants subjected to sulfate deficiency. Indeed, independent microarray analyses of various alleles of mutants in FIERY1 showed similar patterns of gene expression as in sulfate deficient plants. This was not caused by alterations in signalling, as the fou8 mutants contained significantly lower levels of sulfate and glutathione and, consequently, of total elemental sulfur. Analysis of mutants with altered levels of sulfate and glutathione confirmed the correlation of sulfate deficiency-like gene expression pattern with low internal sulfate but not low glutathione. The changes in sulfur metabolism in fou8 correlated with massive increases in 3'-phosphoadenosine 5'-phosphate levels. The analysis of fou8 thus revealed that sulfate starvation response is triggered by a decrease in internal sulfate as opposed to external sulfate availability and that the presence of desulfo-glucosinolates does not induce the glucosinolate synthesis network. However, as well as resolving these important questions on the regulation of sulfate assimilation in plants, fou8 has also opened an array of new questions on the links between jasmonate synthesis and sulfur metabolism.", "link"=>"http://www.mendeley.com/research/effects-fou8fry1-mutation-sulfur-metabolism-decreased-internal-sulfate-trigger-sulfate-starvation-re", "reader_count"=>37, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Researcher"=>11, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>14, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>2, "Student > Bachelor"=>2, "Lecturer"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Researcher"=>11, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>14, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>2, "Student > Bachelor"=>2, "Lecturer"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>7, "Agricultural and Biological Sciences"=>27, "Chemistry"=>1, "Earth and Planetary Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Chemistry"=>{"Chemistry"=>1}, "Earth and Planetary Sciences"=>{"Earth and Planetary Sciences"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>27}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>7}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"Argentina"=>1}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/323502", "https://ndownloader.figshare.com/files/323562", "https://ndownloader.figshare.com/files/323612", "https://ndownloader.figshare.com/files/323695", "https://ndownloader.figshare.com/files/323778", "https://ndownloader.figshare.com/files/323849"], "description"=>"<div><p>The <em>fou8</em> loss of function allele of adenosine bisphosphate phosphatase FIERY1 results in numerous phenotypes including the increased enzymatic oxygenation of fatty acids and increased jasmonate synthesis. Here we show that the mutation causes also profound alterations of sulfur metabolism. The <em>fou8</em> mutants possess lower levels of sulfated secondary compounds, glucosinolates, and accumulate the desulfo-precursors similar to previously described mutants in adenosine 5′phosphosulfate kinase. Transcript levels of genes involved in sulfate assimilation differ in <em>fou8</em> compared to wild type Col-0 plants and are similar to plants subjected to sulfate deficiency. Indeed, independent microarray analyses of various alleles of mutants in <em>FIERY1</em> showed similar patterns of gene expression as in sulfate deficient plants. This was not caused by alterations in signalling, as the <em>fou8</em> mutants contained significantly lower levels of sulfate and glutathione and, consequently, of total elemental sulfur. Analysis of mutants with altered levels of sulfate and glutathione confirmed the correlation of sulfate deficiency-like gene expression pattern with low internal sulfate but not low glutathione. The changes in sulfur metabolism in <em>fou8</em> correlated with massive increases in 3′-phosphoadenosine 5′-phosphate levels. The analysis of <em>fou8</em> thus revealed that sulfate starvation response is triggered by a decrease in internal sulfate as opposed to external sulfate availability and that the presence of desulfo-glucosinolates does not induce the glucosinolate synthesis network. However, as well as resolving these important questions on the regulation of sulfate assimilation in plants, <em>fou8</em> has also opened an array of new questions on the links between jasmonate synthesis and sulfur metabolism.</p> </div>", "links"=>[], "tags"=>["effects", "mutation", "sulfur", "decreased", "sulfate", "starvation"], "article_id"=>123844, "categories"=>["Biochemistry", "Genetics", "Cell Biology"], "users"=>["Bok-Rye Lee", "Stine Huseby", "Anna Koprivova", "Aurore Chételat", "Markus Wirtz", "Sam T. Mugford", "Emily Navid", "Charles Brearley", "Shikha Saha", "Richard Mithen", "Rüdiger Hell", "Edward E. Farmer", "Stanislav Kopriva"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0039425.s001", "https://dx.doi.org/10.1371/journal.pone.0039425.s002", "https://dx.doi.org/10.1371/journal.pone.0039425.s003", "https://dx.doi.org/10.1371/journal.pone.0039425.s004", "https://dx.doi.org/10.1371/journal.pone.0039425.s005", "https://dx.doi.org/10.1371/journal.pone.0039425.s006"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Effects_of_fou8_fry1_Mutation_on_Sulfur_Metabolism_Is_Decreased_Internal_Sulfate_the_Trigger_of_Sulfate_Starvation_Response_/123844", "title"=>"Effects of <em>fou8/fry1</em> Mutation on Sulfur Metabolism: Is Decreased Internal Sulfate the Trigger of Sulfate Starvation Response?", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-06-18 01:04:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/623322"], "description"=>"<p>Scheme of involvement of FOU8 in sulfur metabolism.</p>", "links"=>[], "tags"=>["fou8", "sulfur"], "article_id"=>293800, "categories"=>["Biochemistry", "Genetics", "Plant Biology"], "users"=>["Bok-Rye Lee", "Stine Huseby", "Anna Koprivova", "Aurore Chételat", "Markus Wirtz", "Sam T. Mugford", "Emily Navid", "Charles Brearley", "Shikha Saha", "Richard Mithen", "Rüdiger Hell", "Edward E. Farmer", "Stanislav Kopriva"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0039425.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Scheme_of_involvement_of_FOU8_in_sulfur_metabolism_/293800", "title"=>"Scheme of involvement of FOU8 in sulfur metabolism.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-06-18 01:03:20"}
  • {"files"=>["https://ndownloader.figshare.com/files/623421"], "description"=>"<p>Col-0, <i>fou8</i>, <i>apk1 apk2</i>, and <i>fou8 apk1 apk2</i> plants were grown for 5 weeks in controlled environment room. The total content of <b>A</b> glucosinolates and <b>B</b> desulfo-glucosinolates was measured in leaves. <b>C</b> Total RNA was isolated from leaves and the transcript levels of six genes involved in glucosinolate synthesis was determined by quantitative RT-PCR. The qRT-PCR reactions were performed in triplicate for each biological sample. The values in Col-0 were set to 1 for all genes. Results are presented as means ± SE from six pools of three individual plants grown in two independent experiments. Different letters mark values significantly different at P<0.05; asterisks mark values significantly different from Col-0 at P<0.05.</p>", "links"=>[], "tags"=>["affected", "glucosinolate"], "article_id"=>293891, "categories"=>["Biochemistry", "Genetics", "Plant Biology"], "users"=>["Bok-Rye Lee", "Stine Huseby", "Anna Koprivova", "Aurore Chételat", "Markus Wirtz", "Sam T. Mugford", "Emily Navid", "Charles Brearley", "Shikha Saha", "Richard Mithen", "Rüdiger Hell", "Edward E. Farmer", "Stanislav Kopriva"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0039425.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_fou8_is_affected_in_glucosinolate_synthesis_/293891", "title"=>"<i>fou8</i> is affected in glucosinolate synthesis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-06-18 01:04:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/623550"], "description"=>"<p>Col-0, <i>fou8</i>, <i>apk3</i>, and <i>fou8 apk3</i> plants were grown for 5 weeks in controlled environment room. The total content of <b>A</b> glucosinolates and <b>B</b> desulfo-glucosinolates was measured in leaves. <b>C</b> Total RNA was isolated from leaves and the transcript levels of six genes involved in glucosinolate synthesis was determined by quantitative RT-PCR. The qRT-PCR reactions were performed in triplicate for each biological sample. The values in Col-0 were set to 1 for all genes. Results are presented as means ± SE from six pools of three individual plants grown in two independent experiments. Different letters mark values significantly different at P<0.05; asterisks mark values significantly different from Col-0 at P<0.05.</p>", "links"=>[], "tags"=>["synthesis", "mutant", "cytosolic", "aps"], "article_id"=>294020, "categories"=>["Biochemistry", "Genetics", "Plant Biology"], "users"=>["Bok-Rye Lee", "Stine Huseby", "Anna Koprivova", "Aurore Chételat", "Markus Wirtz", "Sam T. Mugford", "Emily Navid", "Charles Brearley", "Shikha Saha", "Richard Mithen", "Rüdiger Hell", "Edward E. Farmer", "Stanislav Kopriva"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0039425.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Glucosinolate_synthesis_in_fou8_mutant_with_and_without_cytosolic_APS_kinase_/294020", "title"=>"Glucosinolate synthesis in <i>fou8</i> mutant with and without cytosolic APS kinase.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-06-18 01:07:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/623707"], "description"=>"<p>Col-0 and <i>fou8</i> plants were grown for 5 weeks in controlled environment room. Leaves were harvested and <b>A</b> APR activity was determined. Levels of <b>B</b> cysteine <b>C</b> glutathione, and <b>D</b> sulfate were measured by HPLC. Results are presented as means ± SE from six individual plants, grown in two independent experiments. Asterisks mark values significantly different from Col-0 at P<0.05.</p>", "links"=>[], "tags"=>["sulfur-containing", "compounds"], "article_id"=>294180, "categories"=>["Biochemistry", "Genetics", "Plant Biology"], "users"=>["Bok-Rye Lee", "Stine Huseby", "Anna Koprivova", "Aurore Chételat", "Markus Wirtz", "Sam T. Mugford", "Emily Navid", "Charles Brearley", "Shikha Saha", "Richard Mithen", "Rüdiger Hell", "Edward E. Farmer", "Stanislav Kopriva"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0039425.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Accumulation_of_sulfur_containing_compounds_in_fou8_/294180", "title"=>"Accumulation of sulfur-containing compounds in <i>fou8</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-06-18 01:09:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/623869"], "description"=>"<p>Col-0, <i>fou8</i>, <i>cad2</i>, <i>rax1</i>, and <i>sultr1;2</i> plants were grown for 2.5 weeks on MS-agarose plates. Relative mRNA levels of <i>ATPS4</i> and <i>APR1</i> in <b>A</b> leaves and <b>B</b> roots and of <i>LS2</i> (At5g48850) and <i>LS5</i> (At5g26220) in <b>C</b> leaves and <b>D</b> roots and genes of glucosinolate synthesis in <b>E</b> leaves were determined. The qRT-PCR reactions were performed in triplicate, the values in Col-0 were set to 1 for all genes. Results are presented as means ± SE from six biological replicates from plants grown in two independent experiments. Different letters mark values significantly different at P<0.05 or in <b>E</b> asterisks show values significantly (P<0.05) different from Col-0.</p>", "links"=>[], "tags"=>["mrna", "levels", "genes", "sulfur"], "article_id"=>294337, "categories"=>["Biochemistry", "Genetics", "Plant Biology"], "users"=>["Bok-Rye Lee", "Stine Huseby", "Anna Koprivova", "Aurore Chételat", "Markus Wirtz", "Sam T. Mugford", "Emily Navid", "Charles Brearley", "Shikha Saha", "Richard Mithen", "Rüdiger Hell", "Edward E. Farmer", "Stanislav Kopriva"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0039425.g005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparison_of_mRNA_levels_of_key_genes_of_sulfur_metabolism_/294337", "title"=>"Comparison of mRNA levels of key genes of sulfur metabolism.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-06-18 01:12:17"}
  • {"files"=>["https://ndownloader.figshare.com/files/624057"], "description"=>"<p>The various Arabidopsis lines were grown for 5 weeks in controlled environment room. Leaf juice was incubated for 2 min with 1-[<sup>14</sup>C]18∶3. Products were separated by thin layer chromatography and the radioactivity quantified. The rate of oxygenation is expressed as % of radioactivity in 18∶3-α-ketol. Results are presented as means ± SE from six individual plants grown in two independent experiments, different letters mark values significantly different at P<0.05.</p>", "links"=>[], "tags"=>["fatty", "oxygenation"], "article_id"=>294524, "categories"=>["Biochemistry", "Genetics", "Plant Biology"], "users"=>["Bok-Rye Lee", "Stine Huseby", "Anna Koprivova", "Aurore Chételat", "Markus Wirtz", "Sam T. Mugford", "Emily Navid", "Charles Brearley", "Shikha Saha", "Richard Mithen", "Rüdiger Hell", "Edward E. Farmer", "Stanislav Kopriva"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0039425.g006"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Variation_in_fatty_acid_oxygenation_rate_in_fou8_related_genotypes_/294524", "title"=>"Variation in fatty acid oxygenation rate in <i>fou8</i> related genotypes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-06-18 01:15:24"}
  • {"files"=>["https://ndownloader.figshare.com/files/624208"], "description"=>"<p>The various Arabidopsis lines were grown for 5 weeks in controlled environment room. Leaves were harvested and the levels of sulfur-containing compounds were determined by HPLC. <b>A</b> sulfate, <b>B</b> glutathione, <b>C</b> glucosinolates, and <b>D</b> desulfo-glucosinolates. Results are presented as means ± SE from six individual plants grown in two independent experiments with three replicates each. Different letters mark values significantly different at P<0.05; ND  =  not detectable.</p>", "links"=>[], "tags"=>["sulfur-containing", "compounds"], "article_id"=>294679, "categories"=>["Biochemistry", "Genetics", "Plant Biology"], "users"=>["Bok-Rye Lee", "Stine Huseby", "Anna Koprivova", "Aurore Chételat", "Markus Wirtz", "Sam T. Mugford", "Emily Navid", "Charles Brearley", "Shikha Saha", "Richard Mithen", "Rüdiger Hell", "Edward E. Farmer", "Stanislav Kopriva"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0039425.g007"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Accumulation_of_sulfur_containing_compounds_in_fou8_related_genotypes_/294679", "title"=>"Accumulation of sulfur-containing compounds in <i>fou8</i> related genotypes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-06-18 01:17:59"}
  • {"files"=>["https://ndownloader.figshare.com/files/624347"], "description"=>"<p>The various Arabidopsis lines were grown for 5 weeks in controlled environment room. Total RNA was isolated from leaves and the transcript levels of genes involved in sulfur metabolism, glucosinolate synthesis, and jasmonate synthesis were determined by quantitative RT-PCR. The qRT-PCR reactions were performed in triplicate for each of the six independent biological samples from plants grown in two independent experiments. Results are presented as a heat map of relative mRNA levels compared to Col-0. For comparison, sulfate levels are presented in the same way on the far right.</p>", "links"=>[], "tags"=>["arabidopsis"], "article_id"=>294823, "categories"=>["Biochemistry", "Genetics", "Plant Biology"], "users"=>["Bok-Rye Lee", "Stine Huseby", "Anna Koprivova", "Aurore Chételat", "Markus Wirtz", "Sam T. Mugford", "Emily Navid", "Charles Brearley", "Shikha Saha", "Richard Mithen", "Rüdiger Hell", "Edward E. Farmer", "Stanislav Kopriva"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0039425.g008"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_analysis_of_Arabidopsis_lines_/294823", "title"=>"Expression analysis of Arabidopsis lines.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-06-18 01:20:23"}
  • {"files"=>["https://ndownloader.figshare.com/files/624493"], "description"=>"<p>The various Arabidopsis lines were grown for 5 weeks in controlled environment room. Leaves were harvested and the levels of <b>A</b> PAP and <b>B</b> PAPS were determined by HPLC. Results are presented as means ± SD from four individual plants, grown in two independent experiments. Asterisks represents levels under detection limit, different letters mark values significantly different at P<0.05.</p>", "links"=>[], "tags"=>["pap", "paps"], "article_id"=>294962, "categories"=>["Biochemistry", "Genetics", "Plant Biology"], "users"=>["Bok-Rye Lee", "Stine Huseby", "Anna Koprivova", "Aurore Chételat", "Markus Wirtz", "Sam T. Mugford", "Emily Navid", "Charles Brearley", "Shikha Saha", "Richard Mithen", "Rüdiger Hell", "Edward E. Farmer", "Stanislav Kopriva"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0039425.g009"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Accumulation_of_PAP_and_PAPS_in_fou8_related_genotypes_/294962", "title"=>"Accumulation of PAP and PAPS in <i>fou8</i> related genotypes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-06-18 01:22:42"}
  • {"files"=>["https://ndownloader.figshare.com/files/624675"], "description"=>"<p>WT Col-0 and mutants <i>fou8</i>, <i>fou2</i>, and <i>aos</i> were grown for 3 weeks on MS-phytagel vertical plates in controlled environment room. The seedlings were incubated for four hours with their roots submerged in nutrient solution adjusted to sulfate concentration of 0.2 mM and supplemented with 6.7 μCi [<sup>35</sup>S]sulfate. Shoot and root material was harvested separately, and the flux was determined as incorporation of <sup>35</sup>S from [<sup>35</sup>S] sulfate to thiols and proteins. <b>A</b> sulfate uptake, <b>B</b> Percentage of <sup>35</sup>S transported to leaves from the [<sup>35</sup>S]sulfate taken up, <b>C</b> relative flux through the sulfate assimilation in the leaves calculated as % of incorporation in thiols and proteins from total [<sup>35</sup>S]sulfate taken up. Results are presented as means ± SE from six independent pools of 8 seedlings grown in two independent experiments. Values marked with an asterisk show significant (P≤0.05) difference from Col-0.</p>", "links"=>[], "tags"=>["uptake", "flux", "sulfate", "assimilation"], "article_id"=>295140, "categories"=>["Biochemistry", "Genetics", "Plant Biology"], "users"=>["Bok-Rye Lee", "Stine Huseby", "Anna Koprivova", "Aurore Chételat", "Markus Wirtz", "Sam T. Mugford", "Emily Navid", "Charles Brearley", "Shikha Saha", "Richard Mithen", "Rüdiger Hell", "Edward E. Farmer", "Stanislav Kopriva"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0039425.g010"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sulfate_uptake_and_flux_through_sulfate_assimilation_in_fou8_and_related_mutants_/295140", "title"=>"Sulfate uptake and flux through sulfate assimilation in <i>fou8</i> and related mutants.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-06-18 01:25:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/624796"], "description"=>"<p>Col-0 and <i>fou8</i> plants were grown for 5 weeks in controlled environment room. Leaves were harvested and the levels of glucosinolates (µmol/g FW) were determined by HPLC. Results are presented as means ± SD from three individual plants characteristic of four independent experimental repeats. Ratios of individual glucosinolates different from the ratio of total glucosinolates by more than 25% are printed bold.</p>", "links"=>[], "tags"=>["glucosinolates", "col-0"], "article_id"=>295269, "categories"=>["Biochemistry", "Genetics", "Plant Biology"], "users"=>["Bok-Rye Lee", "Stine Huseby", "Anna Koprivova", "Aurore Chételat", "Markus Wirtz", "Sam T. Mugford", "Emily Navid", "Charles Brearley", "Shikha Saha", "Richard Mithen", "Rüdiger Hell", "Edward E. Farmer", "Stanislav Kopriva"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0039425.t001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Levels_of_individual_glucosinolates_in_Col_0_and_fou8_/295269", "title"=>"Levels of individual glucosinolates in Col-0 and <i>fou8</i>.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-06-18 01:27:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/624859"], "description"=>"<p>Col-0 and <i>fou8</i> plants were grown for 30 days in soil in controlled environment room. Whole rosettes were harvested and the mineral levels were determined by X-ray fluorescence spectrophotometry as % of dry weight. Results from one of two independent experiments are presented as means ± SD from three individual plants. Values substantially different between the two genotypes (P<0.05) are marked by asterisks.</p>", "links"=>[], "tags"=>["col-0"], "article_id"=>295328, "categories"=>["Biochemistry", "Genetics", "Plant Biology"], "users"=>["Bok-Rye Lee", "Stine Huseby", "Anna Koprivova", "Aurore Chételat", "Markus Wirtz", "Sam T. Mugford", "Emily Navid", "Charles Brearley", "Shikha Saha", "Richard Mithen", "Rüdiger Hell", "Edward E. Farmer", "Stanislav Kopriva"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0039425.t003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mineral_content_of_Col_0_and_fou8_/295328", "title"=>"Mineral content of Col-0 and <i>fou8</i>.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-06-18 01:28:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/624918"], "description"=>"<p>Col-0 and <i>fou8</i> plants were grown for 5 weeks in controlled environment room. Total RNA was isolated from leaves and the transcript levels of genes involved in sulfur metabolism was determined by quantitative RT-PCR. In two independent experiments qRT-PCR reactions were performed in triplicate for each of the three independent biological samples. The values in Col-0 were set to 1 for all genes. Results of one experiment are presented as means ± SD from three pools of three individual plants. P-values obtained by Student's T-test are also given.</p>", "links"=>[], "tags"=>["sulfate", "assimilation", "genes"], "article_id"=>295391, "categories"=>["Biochemistry", "Genetics", "Plant Biology"], "users"=>["Bok-Rye Lee", "Stine Huseby", "Anna Koprivova", "Aurore Chételat", "Markus Wirtz", "Sam T. Mugford", "Emily Navid", "Charles Brearley", "Shikha Saha", "Richard Mithen", "Rüdiger Hell", "Edward E. Farmer", "Stanislav Kopriva"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0039425.t002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Relative_expression_of_sulfate_assimilation_genes_in_fou8_/295391", "title"=>"Relative expression of sulfate assimilation genes in <i>fou8</i>.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-06-18 01:29:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/624987"], "description"=>"<p>Col-0, <i>fou8</i>, <i>cad2</i>, <i>rax1</i>, and <i>sultr1;2</i> plants were grown for 2.5 weeks on MS-agarose plates. Sulfate, cysteine, glutathione in leaves and roots, and total glucosinolates and desulfo-glucosinolates levels in leaves were determined by HPLC. Results from one of two independent experiments are presented as means ± SD from three biological replicates, different letters mark values significantly different at P<0.05. n.d., not determined.</p>", "links"=>[], "tags"=>["sulfur-containing", "metabolites", "genotypes", "affected", "sulfur"], "article_id"=>295453, "categories"=>["Biochemistry", "Genetics", "Plant Biology"], "users"=>["Bok-Rye Lee", "Stine Huseby", "Anna Koprivova", "Aurore Chételat", "Markus Wirtz", "Sam T. Mugford", "Emily Navid", "Charles Brearley", "Shikha Saha", "Richard Mithen", "Rüdiger Hell", "Edward E. Farmer", "Stanislav Kopriva"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0039425.t004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Contents_of_sulfur_containing_metabolites_in_genotypes_affected_in_sulfur_metabolism_/295453", "title"=>"Contents of sulfur-containing metabolites in genotypes affected in sulfur metabolism.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-06-18 01:30:53"}

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Relative Metric

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