Noscapine Induced Apoptosis via Downregulation of Survivin in Human Neuroblastoma Cells Having Wild Type or Null p53
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{"title"=>"Noscapine induced apoptosis via downregulation of survivin in human neuroblastoma cells having wild type or null p53", "type"=>"journal", "authors"=>[{"first_name"=>"Shiwang", "last_name"=>"Li", "scopus_author_id"=>"8287120600"}, {"first_name"=>"Jing", "last_name"=>"He", "scopus_author_id"=>"34769976900"}, {"first_name"=>"Shuai", "last_name"=>"Li", "scopus_author_id"=>"55158712000"}, {"first_name"=>"Guoqing", "last_name"=>"Cao", "scopus_author_id"=>"35572434200"}, {"first_name"=>"Shaotao", "last_name"=>"Tang", "scopus_author_id"=>"16023636800"}, {"first_name"=>"Qiangsong", "last_name"=>"Tong", "scopus_author_id"=>"8385021300"}, {"first_name"=>"Harish C.", "last_name"=>"Joshi", "scopus_author_id"=>"7103239775"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"365334853", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "sgr"=>"84864380566", "issn"=>"19326203", "pmid"=>"22848370", "scopus"=>"2-s2.0-84864380566", "doi"=>"10.1371/journal.pone.0040076"}, "id"=>"f7172665-49b1-3615-bdcf-9ad8a3b9c729", "abstract"=>"Neuroblastoma is the most common extracranial solid tumor of childhood. It accounts for 15% of pediatric cancer deaths. Chemotherapy is the mainstay of treatment in children with advanced neuroblastoma. Noscapine, a nontoxic natural compound, can trigger apoptosis in many cancer types. We now show that p53 is dispensable for Noscapine-induced cell death in neuroblastoma cell lines, proapoptotic response to this promising chemopreventive agent is mediated by suppression of survivin protein expression. The Noscapine treatment increased levels of total and Ser(15)-phosphorylated p53 protein in SK-SY5Y cells, but the proapoptotic response to this agent was maintained even after knockdown of the p53 protein level. Exposure of SK-SY5Y and LA1-5S cells to Noscapine resulted in a marked decrease in protein and mRNA level of survivin as early as 12 hours after treatment. Ectopic expression of survivin conferred statistically significant protection against Noscapine-mediated cytoplasmic histone-associated apoptotic DNA fragmentation. Also, the Noscapine-induced apoptosis was modestly but statistically significantly augmented by RNA interference of survivin in both cell lines. Furthermore, Noscapine-induced apoptotic cell death was associated with activation of caspase-3 and cleavage of PARP. In conclusion, the present study provides novel insight into the molecular circuitry of Noscapine-induced apoptosis to indicate suppression of survivin expression as a critical mediator of this process.", "link"=>"http://www.mendeley.com/research/noscapine-induced-apoptosis-via-downregulation-survivin-human-neuroblastoma-cells-having-wild-type-n", "reader_count"=>10, "reader_count_by_academic_status"=>{"Researcher"=>3, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>1, "Other"=>1, "Student > Master"=>2}, "reader_count_by_user_role"=>{"Researcher"=>3, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>1, "Other"=>1, "Student > Master"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>3, "Agricultural and Biological Sciences"=>2, "Medicine and Dentistry"=>2, "Chemistry"=>1, "Social Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Chemistry"=>{"Chemistry"=>1}, "Social Sciences"=>{"Social Sciences"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>2}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"India"=>1}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/602259"], "description"=>"<p>Immunoblot analysis of SK-SY5Y and LA1-5S cells treated with noscapine for 0, 12, 24 and 48 hrs. After the indicated times, cells were lysed and total protein was extracted, separated by SDS-PAGE, electrotransferred onto polyvinylidene difluoride membrane, and subjected to immunoblotting with the indicated primary antibodies followed by incubation with horseradish peroxidase- conjugated secondary antibodies. β-actin was used as a loading control.</p>", "links"=>[], "tags"=>["induced", "apoptosis", "activation", "caspase-", "parp"], "article_id"=>272752, "categories"=>["Cancer", "Molecular Biology", "Chemistry"], "users"=>["Shiwang Li", "Jing He", "Shuai Li", "Guoqing Cao", "Shaotao Tang", "Qiangsong Tong", "Harish C. Joshi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0040076.g007", "stats"=>{"downloads"=>3, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Noscapine_induced_apoptosis_via_activation_of_caspase_3_and_PARP_cleavage_/272752", "title"=>"Noscapine induced apoptosis via activation of caspase- 3 and PARP cleavage.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 01:49:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/602010"], "description"=>"<p>Noscapine-inducedapoptosis.Immunoblotting for survivin protein using lysates from NB1691(A) and LA1-55N (C) cells transiently transfected with empty pcDNA3.1 vector or pcDNA3.1 vector encoding for survivin and treated for 24 h with DMSO (control) or 50 µmol/L Noscapine. The numbers above the immunoreactive bands represent change in protein level relative to empty vector–transfected cells treated with DMSO (lane 1). Cytoplasmic histone-associated apoptotic DNA fragmentation in NB1691 (B) and LA1-55N (D) cells transiently transfected with empty pcDNA3.1 vector or vector encoding for survivin and treated for 24 h with DMSO (control) or 50 µmol/L Noscapine. Columns, mean (n = 3); bars, SD. Significantly different (P<0.05) compared with empty vector–transfected cells treated with DMSO (a) and empty vector–transfected cells treated with Noscapine (b) by one-way ANOVA followed by Bonferroni’s test. Comparable results were observed in three independent experiments. Representative data from one such experiment are shown.</p>", "links"=>[], "tags"=>["survivin", "conferred", "noscapine-induced"], "article_id"=>272497, "categories"=>["Cancer", "Molecular Biology", "Chemistry"], "users"=>["Shiwang Li", "Jing He", "Shuai Li", "Guoqing Cao", "Shaotao Tang", "Qiangsong Tong", "Harish C. Joshi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0040076.g005", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Overexpression_of_survivin_conferred_protection_Noscapine_induced_apoptosis_/272497", "title"=>"Overexpression of survivin conferred protection Noscapine-induced apoptosis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 01:48:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/601451"], "description"=>"<p><b>A</b>. A panel of Neuroblastoma cells (SK-SY5Y, SH-EP1, SK-N-MC, SK-N-AS, LA1-55N, LA1-5S, NB1643, NB1691, SK-N-SH and IMR32) were treated with gradient concentrations of Noscapine for 72 hrs and the IC50 values were evaluated by the sulforhodamine B (SRB) <i>in vitro</i> cell proliferation assay. The values and error bars shown in the graph represent average and standard deviations, respectively, of three independent experiments (p<0.05). <b>B</b>. Phase-contrast images (20×) of SK-SY5Y cells treated with 50 µM Noscapine for 0, and 48 hrs.</p>", "links"=>[], "tags"=>["suppresses", "proliferation", "neuroblastoma"], "article_id"=>271938, "categories"=>["Cancer", "Molecular Biology", "Chemistry"], "users"=>["Shiwang Li", "Jing He", "Shuai Li", "Guoqing Cao", "Shaotao Tang", "Qiangsong Tong", "Harish C. Joshi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0040076.g001", "stats"=>{"downloads"=>3, "page_views"=>24, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Noscapine_suppresses_proliferation_of_human_neuroblastoma_cells_/271938", "title"=>"Noscapine suppresses proliferation of human neuroblastoma cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 01:44:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/601601"], "description"=>"<p><b>A</b>. Effect of 25 µM EM011 on cell-cycle progression of A549 cells over time shown in a three-dimensional disposition. X-axis, intensity of propidium iodide fluorescence, is indicative of the total DNA content of cells in various phases of the cell-cycle. Y-axis, number of cells detected for a given DNA content. Z-axis, time points (i.e., 0, 12, 24, and 48 hrs). Representative results of three independent experiments. <b>B</b>. Quantitative graphical representation of the percentage of G2/M and sub-G1 cell population. Points, average of three independent experiments; bars, SD (p<0.05). C. Confocal immunofluorescence analyses of cells. Microtubules and DNA were visualized using fluorescent anti-alpha-tubulin antibody (green) and fluorescent DNA dye propidium iodide (red). SK-SY5Y cells begin to show mitotic arrest as early as 12 hours after drug treatment and begin to show disorganized arrest and chromatin decondensation or even death at 48 hours of treatment.</p>", "links"=>[], "tags"=>["perturbs", "cell-cycle", "progression", "sk-sy5y", "cells"], "article_id"=>272085, "categories"=>["Cancer", "Molecular Biology", "Chemistry"], "users"=>["Shiwang Li", "Jing He", "Shuai Li", "Guoqing Cao", "Shaotao Tang", "Qiangsong Tong", "Harish C. Joshi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0040076.g002", "stats"=>{"downloads"=>1, "page_views"=>21, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Noscapine_perturbs_cell_cycle_progression_of_Sk_SY5Y_cells_and_cause_apoptosis_/272085", "title"=>"Noscapine perturbs cell-cycle progression of Sk-SY5Y cells and cause apoptosis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 01:45:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/602145"], "description"=>"<p>Immunoblotting for survivin using lysates from SK-SY5Y (A) and LA1-5S (C) cells transiently transfected with a control nonspecific siRNA or a survivin-specific siRNA and treated with DMSO (control) or 50 µmol/L Noscapine for 24 h. Cytoplasmic histone-associated DNA fragmentation in SK-SY5Y (B) and LA1-5S (D) cells transiently transfected with a control nonspecific siRNA or a survivin-specific siRNA and treated with DMSO (control) or 50 µmol/L Noscapine for 24 h. Columns, mean (n = 3); bars, SD. Significantly different (P<0.05) compared with control nonspecific siRNA-transfected cells treated with DMSO (a) and control nonspecific siRNA-transfected cells treated with Noscapine (b) by one-way ANOVA followed by Bonferroni’s test. Results were consistent in two experiments, and representative data from one such experiment are shown.</p>", "links"=>[], "tags"=>["endogenous", "survivin", "sk-sy5y", "la1-5s", "cells", "susceptible"], "article_id"=>272631, "categories"=>["Cancer", "Molecular Biology", "Chemistry"], "users"=>["Shiwang Li", "Jing He", "Shuai Li", "Guoqing Cao", "Shaotao Tang", "Qiangsong Tong", "Harish C. Joshi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0040076.g006", "stats"=>{"downloads"=>1, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Knock_down_endogenous_survivin_render_SK_SY5Y_and_LA1_5S_cells_more_susceptible_to_Noscapine_/272631", "title"=>"Knock-down endogenous survivin render SK-SY5Y and LA1-5S cells more susceptible to Noscapine.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 01:48:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/601889"], "description"=>"<p>Immunoblotting for survivin, cIAP1and XIAP using lysates from SK-SY5Y(A) and LA1-5S (B) cells treated with DMSO (control) or Noscapine (50 µmol/L) for the indicated time periods. Immunoblotting for each protein was done at least twice using independently prepared lysates. Representative data from one such experiment are shown. Histograms represent the increase or decrease of survivin mRNA expression in SK-SY5Y (C) and LA1-5S (D) cells incubated with noscapine (50 µM/mL) for 12, 24, and 48h. Measurements were performed in triplicate. Bars represent standard errors and asterisks designate statistically significant results (<i>P</i><0.05).</p>", "links"=>[], "tags"=>["selectively", "survivin", "downregulation", "sk-sy5y", "la1-5s"], "article_id"=>272379, "categories"=>["Cancer", "Molecular Biology", "Chemistry"], "users"=>["Shiwang Li", "Jing He", "Shuai Li", "Guoqing Cao", "Shaotao Tang", "Qiangsong Tong", "Harish C. Joshi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0040076.g004", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Noscapine_selectively_cause_survivin_downregulation_in_SK_SY5Y_and_LA1_5S_cells_/272379", "title"=>"Noscapine selectively cause survivin downregulation in SK-SY5Y and LA1-5S cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 01:47:20"}
  • {"files"=>["https://ndownloader.figshare.com/files/601726"], "description"=>"<p>A, immunoblotting for total and phospho-(Ser<sup>15</sup>)-p53 using lysates from SK-SY5Y cells treated with DMSO (control) or Noscapine (50 µmol/L) for the indicated time periods. B, immunoblotting for p53 using lysates from SK-SY5Y cells transfected with a control nonspecific siRNA or a p53-targeted siRNA and treated for 24 h with DMSO (control) or 50 µmol/L Noscapine. C, cytoplasmic histone-associated apoptotic DNA fragmentation in SK-SY5Y cells transiently transfected with a control nonspecific siRNA or a p53-targeted siRNA and treated for 24 h with DMSO (control) or 50 µmol/L Noscapine. Columns, mean (n = 3); bars, SD. *, significantly different (P<0.05) compared with nonspecific siRNA-transfected cells treated with DMSO by one-way ANOVA followed by Bonferroni’s test. The results were consistent in three independent experiments, and representative data from one such experiment are shown. D, Immunoblotting for survivin using lysates from a series of NB cell lines.</p>", "links"=>[], "tags"=>["p53", "survivin"], "article_id"=>272221, "categories"=>["Cancer", "Molecular Biology", "Chemistry"], "users"=>["Shiwang Li", "Jing He", "Shuai Li", "Guoqing Cao", "Shaotao Tang", "Qiangsong Tong", "Harish C. Joshi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0040076.g003", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Noscapine_cause_p53_independent_survivin_downregulation_/272221", "title"=>"Noscapine cause p53 independent survivin downregulation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 01:46:25"}

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Relative Metric

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