A Neurotoxic Phospholipase A2 Impairs Yeast Amphiphysin Activity and Reduces Endocytosis
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"A neurotoxic phospholipase A2 impairs yeast amphiphysin activity and reduces endocytosis", "type"=>"journal", "authors"=>[{"first_name"=>"Mojca", "last_name"=>"Mattiazzi", "scopus_author_id"=>"35776649800"}, {"first_name"=>"Yidi", "last_name"=>"Sun", "scopus_author_id"=>"7406429651"}, {"first_name"=>"Heimo", "last_name"=>"Wolinski", "scopus_author_id"=>"8506705000"}, {"first_name"=>"Andrej", "last_name"=>"Bavdek", "scopus_author_id"=>"16201856000"}, {"first_name"=>"Toni", "last_name"=>"Petan", "scopus_author_id"=>"22956006100"}, {"first_name"=>"Gregor", "last_name"=>"Anderluh", "scopus_author_id"=>"6701778710"}, {"first_name"=>"Sepp D.", "last_name"=>"Kohlwein", "scopus_author_id"=>"7003518239"}, {"first_name"=>"David G.", "last_name"=>"Drubin", "scopus_author_id"=>"7005173383"}, {"first_name"=>"Igor", "last_name"=>"Križaj", "scopus_author_id"=>"7003382939"}, {"first_name"=>"Uroš", "last_name"=>"Petrovič", "scopus_author_id"=>"55917218200"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "pui"=>"365316274", "doi"=>"10.1371/journal.pone.0040931", "sgr"=>"84864228341", "scopus"=>"2-s2.0-84864228341", "isbn"=>"1932-6203 (Electronic) 1932-6203 (Linking)", "pmid"=>"22844417"}, "id"=>"ac7079eb-2468-35b6-a075-d1a21adfd1a3", "abstract"=>"Background: Presynaptically neurotoxic phospholipases A2 inhibit synaptic vesicle recycling through endocytosis. Principal Findings: Here we provide insight into the action of a presynaptically neurotoxic phospholipase A2 ammodytoxin A (AtxA) on clathrin-dependent endocytosis in budding yeast. AtxA caused changes in the dynamics of vesicle formation and scission from the plasma membrane in a phospholipase activity dependent manner. Our data, based on synthetic dosage lethality screen and the analysis of the dynamics of sites of endocytosis, indicate that AtxA impairs the activity of amphiphysin. Conclusions: We identified amphiphysin and endocytosis as the target of AtxA intracellular activity. We propose that AtxA reduces endocytosis following a mechanism of action which includes both a specific protein-protein interaction and enzymatic activity, and which is applicable to yeast and mammalian cells. Knowing how neurotoxic phospholipases A2 work can open new ways to regulate endocytosis. © 2012 Mattiazzi et al.", "link"=>"http://www.mendeley.com/research/neurotoxic-phospholipase-a2-impairs-yeast-amphiphysin-activity-reduces-endocytosis", "reader_count"=>21, "reader_count_by_academic_status"=>{"Unspecified"=>4, "Professor > Associate Professor"=>3, "Researcher"=>3, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>2, "Student > Master"=>2, "Professor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>4, "Professor > Associate Professor"=>3, "Researcher"=>3, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>2, "Student > Master"=>2, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>5, "Biochemistry, Genetics and Molecular Biology"=>5, "Agricultural and Biological Sciences"=>10, "Chemistry"=>1}, "reader_count_by_subdiscipline"=>{"Chemistry"=>{"Chemistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>10}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>5}, "Unspecified"=>{"Unspecified"=>5}}, "group_count"=>1}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84864228341"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84864228341?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84864228341&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84864228341&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84864228341", "dc:identifier"=>"SCOPUS_ID:84864228341", "eid"=>"2-s2.0-84864228341", "dc:title"=>"A neurotoxic phospholipase A<inf>2</inf> impairs yeast amphiphysin activity and reduces endocytosis", "dc:creator"=>"Mattiazzi M.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"7", "prism:issueIdentifier"=>"7", "prism:pageRange"=>nil, "prism:coverDate"=>"2012-07-23", "prism:coverDisplayDate"=>"23 July 2012", "prism:doi"=>"10.1371/journal.pone.0040931", "citedby-count"=>"4", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Jozef Stefan Institute", "affiliation-city"=>"Ljubljana", "affiliation-country"=>"Slovenia"}, {"@_fa"=>"true", "affilname"=>"University of Toronto Faculty of Medicine", "affiliation-city"=>"Toronto", "affiliation-country"=>"Canada"}], "pubmed-id"=>"22844417", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e40931", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true, "freetoread"=>{"value"=>[{"$"=>"all"}, {"$"=>"publisherfullgold"}, {"$"=>"repository"}, {"$"=>"repositoryvor"}, {"$"=>"repositoryam"}]}, "freetoreadLabel"=>{"value"=>[{"$"=>"All Open Access"}, {"$"=>"Gold"}, {"$"=>"Green"}]}}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0040931", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"23", "xml_views"=>"1", "html_views"=>"149"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"20", "xml_views"=>"5", "html_views"=>"61"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"3", "html_views"=>"24"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"16"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"9"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"0", "xml_views"=>"2", "html_views"=>"3"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"0", "xml_views"=>"3", "html_views"=>"13"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"2", "html_views"=>"14"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"1"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"3", "html_views"=>"4"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"3", "html_views"=>"0"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"0"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"1"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"10", "year"=>"2020", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"11", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"12", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"1", "year"=>"2021", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"2", "year"=>"2021", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"2"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/604341"], "description"=>"<p>All tested neurotoxic sPLA<sub>2</sub>s as well as AtnL bind to both Bmh1 and Bmh2. No binding was detected for the nontoxic AtnI<sub>2</sub> and ppPLA<sub>2</sub>. Responses in response units (RU) were corrected for surface density of Bmh1 and Bmh2 and molecular weight of Bmh1, Bmh2 and sPLA<sub>2</sub>. The bars represent standard deviations of at least three independent measurements. AtxA – ammodytoxin A, AtxB – ammodytoxin B, AtxC – ammodytoxin C, bvPLA<sub>2</sub>– bee venom PLA<sub>2</sub>, bButx – β-bungarotoxin, AtnL – ammodytin L, Tpx – taipoxin, AtnI<sub>2</sub>– ammodytin I<sub>2</sub>, ppPLA<sub>2</sub>– porcine pancreatic PLA<sub>2</sub>.</p>", "links"=>[], "tags"=>["neurotoxic", "yeast", "14-3-3"], "article_id"=>274825, "categories"=>["Biochemistry", "Cell Biology", "Genetics", "Microbiology"], "users"=>["Mojca Mattiazzi", "Yidi Sun", "Heimo Wolinski", "Andrej Bavdek", "Toni Petan", "Gregor Anderluh", "Sepp D. Kohlwein", "David G. Drubin", "Igor Križaj", "Uroš Petrovič"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0040931.g005", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Several_neurotoxic_sPLA_2_s_bind_to_yeast_14_3_3_proteins_/274825", "title"=>"Several neurotoxic sPLA<sub>2</sub>s bind to yeast 14-3-3 proteins.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-07-23 01:20:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/603855"], "description"=>"<p><b>A</b> Average Sla1-GFP patch lifetimes in the wild-type, and cells expressing AtxA, AtnL, and the LV and LW mutants of AtnL. <b>B</b> Analysis of Sla1-GFP dynamics. In the left panels, localizations of Sla1-GFP in the wild-type, AtxA-expressing and AtnL-expressing strains are shown. In the middle panels, representative kymographs are shown. For the AtxA-expressing strain, kymographs for a normally internalized, shallow internalized, retracted and immotile patch are shown. All kymographs are oriented such that the cell interior faces downwards. In the right panels the trackings of three Sla1-GFP patches from the wild-type cells and four patches from AtxA-expressing cells representing one normally internalized, shallow internalized, retracted and immotile patch each, are shown. Noted is also the percentage of their occurrence in the population. For each frame of the movie, the center of the patch was determined. Green rectangles denote the initial position and red rectangles the final position of the patches. Consecutive positions are connected with lines. All traces are oriented with the cell interior facing down. <b>C</b> Average patch lifetime of Sac6-GFP in a strain expressing AtxA from plasmid and corresponding control strain. <b>D</b> Localization of Sac6-GFP in a strain expressing AtxA from plasmid and corresponding control cells. Ctrl – control strain for the integrated AtxA, AtxA – strain expressing AtxA from singe copy integrated in the genome, ctrl<sub>p</sub> – control strain with empty plasmid, AtxA<sub>p</sub>, AtnL<sub>p</sub>, LV<sub>p</sub> and LW<sub>p</sub> – strains expressing the respective proteins from plasmid clones. To determine the average patch lifetimes at least 100 patches from several cells were analyzed unless otherwise noted. The bars on graphs mark the standard deviation. Sla1-GFP movies were taken with a 1 frame/second interval and Sac6-GFP movies with a 4 frames/second interval. Scale bar on the micrographs is 4 µm and on the kymographs 10 seconds. sPLA<sub>2</sub>-expressing strains were compared to the corresponding control strains, and p-values were calculated using the <i>t-</i>test at a 95% confidence interval. Statistically significant differences are denoted with a star (*).</p>", "links"=>[], "tags"=>["affects", "endocytic", "vesicle"], "article_id"=>274340, "categories"=>["Biochemistry", "Cell Biology", "Genetics", "Microbiology"], "users"=>["Mojca Mattiazzi", "Yidi Sun", "Heimo Wolinski", "Andrej Bavdek", "Toni Petan", "Gregor Anderluh", "Sepp D. Kohlwein", "David G. Drubin", "Igor Križaj", "Uroš Petrovič"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0040931.g001", "stats"=>{"downloads"=>3, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_AtxA_affects_the_dynamics_of_endocytic_vesicle_formation_/274340", "title"=>"AtxA affects the dynamics of endocytic vesicle formation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-07-23 01:12:20"}
  • {"files"=>["https://ndownloader.figshare.com/files/604427"], "description"=>"<p>Surface plasmon resonance was used to analyze binding of 14-3-3 proteins to phospholipid vesicles. The response after 180 seconds of dissociation phase is shown. Av. – average response in response units (RU), s.d. – standard deviation in RU of at least three independent measurements.</p>", "links"=>[], "tags"=>["yeast", "14-3-3", "proteins", "phospholipid"], "article_id"=>274915, "categories"=>["Biochemistry", "Cell Biology", "Genetics", "Microbiology"], "users"=>["Mojca Mattiazzi", "Yidi Sun", "Heimo Wolinski", "Andrej Bavdek", "Toni Petan", "Gregor Anderluh", "Sepp D. Kohlwein", "David G. Drubin", "Igor Križaj", "Uroš Petrovič"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0040931.t001", "stats"=>{"downloads"=>1, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Binding_of_yeast_14_3_3_proteins_to_phospholipid_vesicles_/274915", "title"=>"Binding of yeast 14-3-3 proteins to phospholipid vesicles.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-07-23 01:21:55"}
  • {"files"=>["https://ndownloader.figshare.com/files/604138"], "description"=>"<p>Relative uptake rates of LY in AtxA-expressing and control strains with wild-type and different <i>rvs</i> deletion backgrounds. The bars represent standard deviations of at least three independent measurements. AtxA-expressing strains were compared to the corresponding control strains, and p-values were calculated using the <i>t-</i>test at a 95% confidence interval. Statistically significant differences are denoted with a star (*).</p>", "links"=>[], "tags"=>["inhibits", "endocytosis", "rvs161-dependent"], "article_id"=>274626, "categories"=>["Biochemistry", "Cell Biology", "Genetics", "Microbiology"], "users"=>["Mojca Mattiazzi", "Yidi Sun", "Heimo Wolinski", "Andrej Bavdek", "Toni Petan", "Gregor Anderluh", "Sepp D. Kohlwein", "David G. Drubin", "Igor Križaj", "Uroš Petrovič"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0040931.g003", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_AtxA_inhibits_endocytosis_in_an_Rvs161_dependent_manner_/274626", "title"=>"AtxA inhibits endocytosis in an Rvs161-dependent manner.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-07-23 01:17:06"}
  • {"files"=>["https://ndownloader.figshare.com/files/315559"], "description"=>"<div><h3>Background</h3><p>Presynaptically neurotoxic phospholipases A<sub>2</sub> inhibit synaptic vesicle recycling through endocytosis.</p> <h3>Principal Findings</h3><p>Here we provide insight into the action of a presynaptically neurotoxic phospholipase A<sub>2</sub> ammodytoxin A (AtxA) on clathrin-dependent endocytosis in budding yeast. AtxA caused changes in the dynamics of vesicle formation and scission from the plasma membrane in a phospholipase activity dependent manner. Our data, based on synthetic dosage lethality screen and the analysis of the dynamics of sites of endocytosis, indicate that AtxA impairs the activity of amphiphysin.</p> <h3>Conclusions</h3><p>We identified amphiphysin and endocytosis as the target of AtxA intracellular activity. We propose that AtxA reduces endocytosis following a mechanism of action which includes both a specific protein–protein interaction and enzymatic activity, and which is applicable to yeast and mammalian cells. Knowing how neurotoxic phospholipases A<sub>2</sub> work can open new ways to regulate endocytosis.</p> </div>", "links"=>[], "tags"=>["neurotoxic", "phospholipase", "impairs", "yeast", "amphiphysin", "reduces", "endocytosis"], "article_id"=>122286, "categories"=>["Biochemistry", "Cell Biology", "Genetics", "Microbiology"], "users"=>["Mojca Mattiazzi", "Yidi Sun", "Heimo Wolinski", "Andrej Bavdek", "Toni Petan", "Gregor Anderluh", "Sepp D. Kohlwein", "David G. Drubin", "Igor Križaj", "Uroš Petrovič"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0040931", "stats"=>{"downloads"=>12, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/A_Neurotoxic_Phospholipase_A_2_Impairs_Yeast_Amphiphysin_Activity_and_Reduces_Endocytosis/122286", "title"=>"A Neurotoxic Phospholipase A<sub>2</sub> Impairs Yeast Amphiphysin Activity and Reduces Endocytosis", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-07-23 00:38:06"}
  • {"files"=>["https://ndownloader.figshare.com/files/603967"], "description"=>"<p><b>A</b> Average Sla1-GFP patch lifetimes for different <i>rvs</i> deletion strains and <i>yap1802Δ</i> deletion strain expressing AtxA from a single copy integrated in the genome, and corresponding control strains. <b>B</b> Localization of Sla1-GFP in <i>rvs161Δ</i>, <i>rvs167Δ</i>, the <i>rvs161Δ rvs167Δ</i> double deletion strain and <i>yap1802Δ</i>. <b>C</b> Effect of additional expression of either <i>RVS161</i> or <i>RVS167</i> on average Sla1-GFP patch lifetimes for WT and <i>rvs</i> deletion strains expressing AtxA from a single copy integrated in the genome and corresponding control strains. <b>D</b> Average Sla1-GFP patch lifetimes for the wild-type and <i>bmh1Δ</i> and <i>bmh2Δ</i> deletion strains expressing AtxA from plasmid, and corresponding control strains. <b>E</b> Average lifetimes of Rvs161-GFP (left) and Rvs167-GFP (right) patches for the wild-type (ctrl) and AtxA-expressing cells. <b>F</b> Localization of Rvs161-GFP and Rvs167-GFP in AtxA-expressing and control cells. The bars on graphs mark the standard deviation. To determine the average patch lifetimes at least 100 patches from several cells were analyzed. Sla1-GFP movies were taken with a 1 frame/second and Rvs161-GFP and Rvs167-GFP movies with a 4 frames/second interval. Scale bar on the micrographs is 4 µm. AtxA-expressing strains were compared to the corresponding control strains, and p-values were calculated using the <i>t-</i>test at a 95% confidence interval. Statistically significant differences are denoted with a star (*).</p>", "links"=>[], "tags"=>["proteins", "atxa"], "article_id"=>274454, "categories"=>["Biochemistry", "Cell Biology", "Genetics", "Microbiology"], "users"=>["Mojca Mattiazzi", "Yidi Sun", "Heimo Wolinski", "Andrej Bavdek", "Toni Petan", "Gregor Anderluh", "Sepp D. Kohlwein", "David G. Drubin", "Igor Križaj", "Uroš Petrovič"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0040931.g002", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Rvs_proteins_are_needed_for_the_effect_of_AtxA_on_endocytosis_/274454", "title"=>"Rvs proteins are needed for the effect of AtxA on endocytosis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-07-23 01:14:14"}
  • {"files"=>["https://ndownloader.figshare.com/files/604272"], "description"=>"<p>Relative enzymatic activity of AtxA on phospholipid vesicles of different diameters (50 nm, 100 nm and 200 nm) is shown. The measurements were normalized to the phospholipolytic activity on 100 nm vesicles. The bars represent standard deviation of three independent measurements. The differences are statistically significant based on the unpaired t-test at 95% confidence interval.</p>", "links"=>[], "tags"=>["enzymatic", "membrane", "curvature"], "article_id"=>274756, "categories"=>["Biochemistry", "Cell Biology", "Genetics", "Microbiology"], "users"=>["Mojca Mattiazzi", "Yidi Sun", "Heimo Wolinski", "Andrej Bavdek", "Toni Petan", "Gregor Anderluh", "Sepp D. Kohlwein", "David G. Drubin", "Igor Križaj", "Uroš Petrovič"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0040931.g004", "stats"=>{"downloads"=>0, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_AtxA_enzymatic_activity_is_membrane_curvature_dependent_/274756", "title"=>"AtxA enzymatic activity is membrane curvature dependent.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-07-23 01:19:16"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"24", "full-text"=>"21", "pdf"=>"12", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"10", "pdf"=>"7", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"23", "full-text"=>"26", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"5", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"10", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"30", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"0", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"14", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"1", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"4", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"2", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"3", "full-text"=>"12", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"1", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"19", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"1", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"2", "full-text"=>"0", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"2", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"1", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"0", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2021", "month"=>"1"}

Relative Metric

{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[322, 550, 671, 773, 864, 955, 1048, 1135, 1223, 1308, 1387, 1465, 1534, 1602, 1673, 1744, 1813, 1885, 1955, 2026, 2093, 2160, 2228, 2290, 2349]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[319, 556, 679, 785, 881, 970, 1062, 1149, 1236, 1323, 1402, 1474, 1545, 1617, 1681, 1754, 1822, 1892, 1963, 2031, 2099, 2165, 2233, 2299, 2359]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[333, 576, 707, 814, 908, 1004, 1104, 1197, 1280, 1370, 1449, 1531, 1603, 1673, 1742, 1817, 1886, 1954, 2025, 2098, 2171, 2234, 2304, 2365, 2431]}, {"subject_area"=>"/Biology and life sciences/Organisms", "average_usage"=>[331, 557, 677, 777, 868, 960, 1050, 1136, 1223, 1307, 1390, 1466, 1536, 1603, 1673, 1741, 1814, 1889, 1954, 2028, 2096, 2164, 2233, 2305, 2362]}]}
Loading … Spinner
There are currently no alerts