Effects of Deletion of the Streptococcus pneumoniae Lipoprotein Diacylglyceryl Transferase Gene lgt on ABC Transporter Function and on Growth In Vivo
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{"title"=>"Effects of deletion of the Streptococcus pneumoniae lipoprotein diacylglyceryl transferase gene Lgt on ABC transporter function and on growth in vivo", "type"=>"journal", "authors"=>[{"first_name"=>"Suneeta", "last_name"=>"Chimalapati", "scopus_author_id"=>"50261378700"}, {"first_name"=>"Jonathan M.", "last_name"=>"Cohen", "scopus_author_id"=>"7410009516"}, {"first_name"=>"Emilie", "last_name"=>"Camberlein", "scopus_author_id"=>"23468396100"}, {"first_name"=>"Nathanael", "last_name"=>"MacDonald", "scopus_author_id"=>"55617825400"}, {"first_name"=>"Claire", "last_name"=>"Durmort", "scopus_author_id"=>"15724877000"}, {"first_name"=>"Thierry", "last_name"=>"Vernet", "scopus_author_id"=>"7006095302"}, {"first_name"=>"Peter W.M.", "last_name"=>"Hermans", "scopus_author_id"=>"7101909804"}, {"first_name"=>"Timothy", "last_name"=>"Mitchell", "scopus_author_id"=>"7402175039"}, {"first_name"=>"Jeremy S.", "last_name"=>"Brown", "scopus_author_id"=>"35553480100"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "sgr"=>"84864270589", "scopus"=>"2-s2.0-84864270589", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pui"=>"365316799", "doi"=>"10.1371/journal.pone.0041393", "pmid"=>"22911788"}, "id"=>"e6fd5fdf-6839-337f-873a-d49a70c4e7d9", "abstract"=>"Lipoproteins are an important class of surface associated proteins that have diverse roles and frequently are involved in the virulence of bacterial pathogens. As prolipoproteins are attached to the cell membrane by a single enzyme, prolipoprotein diacylglyceryl transferase (Lgt), deletion of the corresponding gene potentially allows the characterisation of the overall importance of lipoproteins for specific bacterial functions. We have used a Δlgt mutant strain of Streptococcus pneumoniae to investigate the effects of loss of lipoprotein attachment on cation acquisition, growth in media containing specific carbon sources, and virulence in different infection models. Immunoblots of triton X-114 extracts, flow cytometry and immuno-fluorescence microscopy confirmed the Δlgt mutant had markedly reduced lipoprotein expression on the cell surface. The Δlgt mutant had reduced growth in cation depleted medium, increased sensitivity to oxidative stress, reduced zinc uptake, and reduced intracellular levels of several cations. Doubling time of the Δlgt mutant was also increased slightly when grown in medium with glucose, raffinose and maltotriose as sole carbon sources. These multiple defects in cation and sugar ABC transporter function for the Δlgt mutant were associated with only slightly delayed growth in complete medium. However the Δlgt mutant had significantly reduced growth in blood or bronchoalveolar lavage fluid and a marked impairment in virulence in mouse models of nasopharyngeal colonisation, sepsis and pneumonia. These data suggest that for S. pneumoniae loss of surface localisation of lipoproteins has widespread effects on ABC transporter functions that collectively prevent the Δlgt mutant from establishing invasive infection.", "link"=>"http://www.mendeley.com/research/effects-deletion-streptococcus-pneumoniae-lipoprotein-diacylglyceryl-transferase-gene-lgt-abc-transp", "reader_count"=>29, "reader_count_by_academic_status"=>{"Researcher"=>4, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>11, "Other"=>1, "Student > Master"=>1, "Student > Bachelor"=>6, "Lecturer"=>2, "Professor"=>2}, "reader_count_by_user_role"=>{"Researcher"=>4, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>11, "Other"=>1, "Student > Master"=>1, "Student > Bachelor"=>6, "Lecturer"=>2, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>3, "Agricultural and Biological Sciences"=>16, "Medicine and Dentistry"=>6, "Chemistry"=>1, "Psychology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>6}, "Chemistry"=>{"Chemistry"=>1}, "Psychology"=>{"Psychology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>16}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"United States"=>1, "Denmark"=>1}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/604052"], "description"=>"<p>(A) Growth of the wild-type and Δ<i>lgt</i> strains in THY assessed by measuring broth culture log<sub>10</sub> OD<sub>580</sub> over time. (B) Proportion of bacteria surviving after incubation with increasing concentrations of DOC. Squares represent the wild-type strain, triangles the Δ<i>lgt</i> strain. Error bars represent SDs, and when not visible are within the symbol.</p>", "links"=>[], "tags"=>["wild-type", "strains", "susceptibility", "doc-induced"], "article_id"=>274541, "categories"=>["Biochemistry", "Microbiology"], "users"=>["Suneeta Chimalapati", "Jonathan M. Cohen", "Emilie Camberlein", "Nathanael MacDonald", "Claire Durmort", "Thierry Vernet", "Peter W. M. Hermans", "Timothy Mitchell", "Jeremy S. Brown"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041393.g005", "stats"=>{"downloads"=>1, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Growth_of_the_wild_type_and_lgt_strains_in_complete_medium_and_susceptibility_to_DOC_induced_lysis_/274541", "title"=>"Growth of the wild-type and Δ<i>lgt</i> strains in complete medium and susceptibility to DOC-induced lysis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 02:00:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/603885"], "description"=>"<p>(A) Flow cytometry analysis of the mean proportion of bacteria positive for IgG binding after incubation with immune sera containing high antibody titres towards lipoproteins of <i>S. pneumoniae</i>. Black columns represent wild-type strain and clear columns represent the Δ<i>lgt</i> strain. Error bars represent SDs and <i>P</i> values were obtained using multiple ANOVA test with post-hoc analysis. (B) Immunofluorescence of the <i>S. pneumoniae</i> wild-type (WT), Δ<i>lgt</i> and Δ<i>PpmA</i> strains using anti- PpmA antibody and FITC conjugated secondary antibody. (C) Immunofluorescence of the <i>S. pneumoniae</i> wild-type (WT), Δ<i>lgt</i> and Δ<i>PhtD</i> strains using anti-PhtD antibody and Cy2 conjugated secondary antibody.</p>", "links"=>[], "tags"=>["mutation", "accessability"], "article_id"=>274377, "categories"=>["Biochemistry", "Microbiology"], "users"=>["Suneeta Chimalapati", "Jonathan M. Cohen", "Emilie Camberlein", "Nathanael MacDonald", "Claire Durmort", "Thierry Vernet", "Peter W. M. Hermans", "Timothy Mitchell", "Jeremy S. Brown"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041393.g003", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_the_lgt_mutation_on_surface_accessability_of_S_pneumoniae_lipoproteins_/274377", "title"=>"Effect of the Δ<i>lgt</i> mutation on surface accessability of <i>S. pneumoniae</i> lipoproteins.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 01:59:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/604639"], "description"=>"<p>n/c = not calculated as the slope of increase of OD<sub>580</sub> was too shallow for an accurate assessment of the doubling time.</p>a<p> = uptake PTS system dependent.</p>a<p> = uptake ABC transporter and PTS system dependent.</p>c<p> = uptake ABC transporter dependent only.</p>", "links"=>[], "tags"=>["times", "wild-type", "strains"], "article_id"=>275130, "categories"=>["Biochemistry", "Microbiology"], "users"=>["Suneeta Chimalapati", "Jonathan M. Cohen", "Emilie Camberlein", "Nathanael MacDonald", "Claire Durmort", "Thierry Vernet", "Peter W. M. Hermans", "Timothy Mitchell", "Jeremy S. Brown"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041393.t003", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Doubling_times_mins_SD_a_for_the_wild_type_and_lgt_strains_in_different_media_/275130", "title"=>"Doubling times (mins) (SD)<sup>a</sup> for the wild-type and Δ<i>lgt</i> strains in different media.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-20 02:03:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/604524"], "description"=>"<p>Strains and primers used in this study.</p>", "links"=>[], "tags"=>["primers"], "article_id"=>275015, "categories"=>["Biochemistry", "Microbiology"], "users"=>["Suneeta Chimalapati", "Jonathan M. Cohen", "Emilie Camberlein", "Nathanael MacDonald", "Claire Durmort", "Thierry Vernet", "Peter W. M. Hermans", "Timothy Mitchell", "Jeremy S. Brown"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041393.t004", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Strains_and_primers_used_in_this_study_/275015", "title"=>"Strains and primers used in this study.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-20 02:02:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/603981"], "description"=>"<p>(A) Uptake of Zn<sup>+2</sup> by the Δ<i>lgt</i> (triangles) and wild-type (filled diamonds) strains measured using a using FluoZin-3 fluorescence. The arrows numbered 1, 2 and 3 indicate the time points at which ZnCl<sub>2</sub>, 1 mM ortho-vanadate and TPEN were added to the strains respectively. Mean (SD) Zn<sup>2+</sup> uptake in RFLU sec<sup>−1</sup> before and after addition of orthovanadate and 10 µm ZnCl<sub>2</sub> are stated next to the corresponding line. For comparison of zinc uptake by wild-type and Δ<i>lgt</i> strains, <i>P</i> = 0.01 using Student’s t-test. (B) Proportion of wild-type and Δ<i>lgt</i> strain bacteria surviving after exposure to 60 mM paraquat for 20 min (clear columns), 40 min (grey columns) and 60 min (black columns). No Δ<i>lgt</i> strain bacteria survived after 60 minutes incubation. For the comparison between wild-type and Δ<i>lgt</i> strains, at 40 and 60 min time points <i>P</i> values were <0.01 and <0.05 (2 way ANOVA with post-hoc tests).</p>", "links"=>[], "tags"=>["phenotypes"], "article_id"=>274470, "categories"=>["Biochemistry", "Microbiology"], "users"=>["Suneeta Chimalapati", "Jonathan M. Cohen", "Emilie Camberlein", "Nathanael MacDonald", "Claire Durmort", "Thierry Vernet", "Peter W. M. Hermans", "Timothy Mitchell", "Jeremy S. Brown"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041393.g004", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cation_dependent_phenotypes_of_the_lgt_strain_/274470", "title"=>"Cation dependent phenotypes of the Δ<i>lgt</i> strain.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 01:59:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/604362"], "description"=>"<p>(A) C3b/iC3b deposition on the wild-type and Δ<i>lgt</i> strains after incubation in 20% serum as measured by geometric mean fluorescent intensity using a flow cytometry assay. (B) Neutrophil association of the wild-type and Δ<i>lgt</i> strains after incubation in 20% serum and human neutrophils as measured by mean relative fluorescent intensity using a flow cytometry assay. (C) Neutrophil killing assays of the wild-type (black columns) and Δ<i>lgt</i> strains (clear columns) after incubation in different concentrations of human serum with fresh human neutrophils (MOI 1 bacteria to 800 neutrophils). There were no statistically significant differences between the wild-type and Δ<i>lgt</i> strains. Data are presented as the percentage of the inoculum surviving after 30 mins incubation. For all panels, data are for mean values with error bars representing SDs. Statistical comparisons were made using unpaired two tailed T tests, and <i>P</i> values inserted for selected data showing significant differences.</p>", "links"=>[], "tags"=>["mutation", "interactions"], "article_id"=>274851, "categories"=>["Biochemistry", "Microbiology"], "users"=>["Suneeta Chimalapati", "Jonathan M. Cohen", "Emilie Camberlein", "Nathanael MacDonald", "Claire Durmort", "Thierry Vernet", "Peter W. M. Hermans", "Timothy Mitchell", "Jeremy S. Brown"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041393.g009", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_the_lgt_mutation_on_interactions_with_neutrophils_/274851", "title"=>"Effects of the Δ<i>lgt</i> mutation on interactions with neutrophils.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 02:01:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/604316"], "description"=>"<p>(A) and (B) Bacterial CFU after 4 hours replication in blood (B) or BALF (B). Data is presented as the mean (SD) bacterial CFU per ml for the wild type (black columns) and the Δ<i>lgt</i> strain (clear columns). <i>P</i> values were obtained using two-tailed Student’s t-tests.</p>", "links"=>[], "tags"=>["wild-type", "strains", "balf"], "article_id"=>274801, "categories"=>["Biochemistry", "Microbiology"], "users"=>["Suneeta Chimalapati", "Jonathan M. Cohen", "Emilie Camberlein", "Nathanael MacDonald", "Claire Durmort", "Thierry Vernet", "Peter W. M. Hermans", "Timothy Mitchell", "Jeremy S. Brown"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041393.g008", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Growth_of_the_wild_type_and_lgt_strains_in_blood_A_or_BALF_B_/274801", "title"=>"Growth of the wild-type and Δ<i>lgt</i> strains in blood (A), or BALF (B).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 02:01:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/604558"], "description"=>"a<p>Length of the amino acids compared.</p>", "links"=>[], "tags"=>["alignments", "sp1412", "amino"], "article_id"=>275042, "categories"=>["Biochemistry", "Microbiology"], "users"=>["Suneeta Chimalapati", "Jonathan M. Cohen", "Emilie Camberlein", "Nathanael MacDonald", "Claire Durmort", "Thierry Vernet", "Peter W. M. Hermans", "Timothy Mitchell", "Jeremy S. Brown"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041393.t001", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Blast_alignments_of_the_Sp1412_Lgt_amino_acid_sequence_to_other_organisms_/275042", "title"=>"Blast alignments of the Sp1412 (Lgt) amino acid sequence to other organisms.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-20 02:03:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/604603"], "description"=>"<p>NA – not available.</p>", "links"=>[], "tags"=>["intracellular", "cation", "contents", "icp-ms", "ppb"], "article_id"=>275087, "categories"=>["Biochemistry", "Microbiology"], "users"=>["Suneeta Chimalapati", "Jonathan M. Cohen", "Emilie Camberlein", "Nathanael MacDonald", "Claire Durmort", "Thierry Vernet", "Peter W. M. Hermans", "Timothy Mitchell", "Jeremy S. Brown"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041393.t002", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Quantification_of_intracellular_S_pneumoniae_and_media_cation_contents_using_ICP_MS_and_expressed_in_Ppb_SD_/275087", "title"=>"Quantification of intracellular <i>S. pneumoniae</i> and media cation contents using ICP-MS and expressed in Ppb (+/− SD).", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-20 02:03:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/604230"], "description"=>"<p>Assessed by measuring broth culture log<sub>10</sub> OD<sub>580</sub> over time. (A) C+Y medium supplemented with 10 mM sucrose and glucose each; (B) C+Y medium supplemented with 10 mM glucose; (C) C+Y medium supplemented with 10 mM maltotriose; and (D) C+Y medium supplemented with 10 mM raffinose. Error bars represent SDs, and when not visible are within the symbol. Filled symbols represent growth of the wild-type strain, empty symbols growth of the Δ<i>lgt</i> strain.</p>", "links"=>[], "tags"=>["wild-type", "strains", "restricted"], "article_id"=>274717, "categories"=>["Biochemistry", "Microbiology"], "users"=>["Suneeta Chimalapati", "Jonathan M. Cohen", "Emilie Camberlein", "Nathanael MacDonald", "Claire Durmort", "Thierry Vernet", "Peter W. M. Hermans", "Timothy Mitchell", "Jeremy S. Brown"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041393.g007", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Growth_of_the_wild_type_and_lgt_strains_in_restricted_carbohydrate_sources_/274717", "title"=>"Growth of the wild-type and Δ<i>lgt</i> strains in restricted carbohydrate sources.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 02:01:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/603800"], "description"=>"<p>(A) Immunoblots of whole cell lysates and the membrane and aqueous phases of triton X-114 extracts of wild-type (WT), Δ<i>lgt</i> strains using antibodies to the <i>S. pneumoniae</i> lipoproteins PiaA, PpmA, SlrA and PiuA. (B) Coomasie blue staining of triton X-114 extracted membrane lipoproteins Δ<i>lgt</i> and wild-type (WT) strains when separated on SDS–PAGE. Lane L, a standard protein ladder with molecular weights ranging from 15 KDa To 80 KDa.</p>", "links"=>[], "tags"=>["mutation", "localisation"], "article_id"=>274286, "categories"=>["Biochemistry", "Microbiology"], "users"=>["Suneeta Chimalapati", "Jonathan M. Cohen", "Emilie Camberlein", "Nathanael MacDonald", "Claire Durmort", "Thierry Vernet", "Peter W. M. Hermans", "Timothy Mitchell", "Jeremy S. Brown"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041393.g002", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_the_lgt_mutation_on_the_localisation_of_S_pneumoniae_lipoproteins_/274286", "title"=>"Effects of the Δ<i>lgt</i> mutation on the localisation of <i>S. pneumoniae</i> lipoproteins.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 01:58:30"}
  • {"files"=>["https://ndownloader.figshare.com/files/603732"], "description"=>"<p>(A) Schematic of the Sp_1410–1413 locus, with the TIGR4 genome gene number and the assigned gene names in parentheses when available. Arrows indicate transcriptional direction and <i>lgt</i> is shaded black. (B) Structure of the Sp_1410–1413 locus in the Δ<i>lgt</i> mutant strain, showing replacement of <i>lgt</i> with an in-frame copy of <i>cat</i> which is shaded grey and position of primers used in (C). (C) Gel red stained agarose gels showing PCR analysis of two separately obtained Δ<i>lgt</i> strains confirming replacement of <i>lgt</i> with <i>cat</i>. Primer pairs (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041393#pone-0041393-t004\" target=\"_blank\">Table 4</a>) and the strain used for each reaction are given above each lane. (D) Gel red stained agarose gels of RT-PCR reactions using internal primers (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041393#pone-0041393-t004\" target=\"_blank\">Table 4</a>) for each gene within the putative Sp_1410–1413 operon, confirming the non-polar deletion of <i>lgt</i>. Reactions not containing reverse transcriptase gave no products (not shown), and L marks the DNA ladder size marker with sizes listed on the left.</p>", "links"=>[], "tags"=>["microbiology", "Biochemistry"], "article_id"=>274219, "categories"=>["Biochemistry", "Microbiology"], "users"=>["Suneeta Chimalapati", "Jonathan M. Cohen", "Emilie Camberlein", "Nathanael MacDonald", "Claire Durmort", "Thierry Vernet", "Peter W. M. Hermans", "Timothy Mitchell", "Jeremy S. Brown"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041393.g001", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Construction_of_the_lgt_strain_/274219", "title"=>"Construction of the Δ<i>lgt</i> strain.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 01:58:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/604124"], "description"=>"<p>Assessed by measuring broth culture log<sub>10</sub> OD<sub>580</sub> over time. (A) to (C) Growth in cation depleted chelex-THY and (D) to (E) in CDM with and without cation supplementation: (A) and (D) with and without Mn<sup>2+</sup> supplementation; (C) and (E) with and without Fe<sup>2+</sup> supplementation; and (D) and (F) with and without combined Mn<sup>2+</sup>, Fe<sup>2+</sup> and Zn<sup>2+</sup> supplementation. Filled symbols represent growth of the wild-type strain, empty symbols growth of the Δ<i>lgt</i> strain. Squares represent growth in unsupplemented media, inverted triangles in media supplemented with 50 µM Mn<sup>2+</sup>, diamonds in media supplemented with 50 µM Fe<sup>+2</sup> and circles in media supplemented with 50 µM Mn<sup>+2</sup>, Fe<sup>+2</sup> and Zn<sup>+2</sup>.</p>", "links"=>[], "tags"=>["wild-type", "strains", "cation", "depleted"], "article_id"=>274607, "categories"=>["Biochemistry", "Microbiology"], "users"=>["Suneeta Chimalapati", "Jonathan M. Cohen", "Emilie Camberlein", "Nathanael MacDonald", "Claire Durmort", "Thierry Vernet", "Peter W. M. Hermans", "Timothy Mitchell", "Jeremy S. Brown"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041393.g006", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Growth_of_the_wild_type_and_lgt_strains_in_cation_depleted_media_/274607", "title"=>"Growth of the wild-type and Δ<i>lgt</i> strains in cation depleted media.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 02:00:23"}
  • {"files"=>["https://ndownloader.figshare.com/files/604451"], "description"=>"<p>(A) CIs for the Δ<i>lgt</i> strain versus the wild-type strain in mouse models of septicaemia at 24 hours (IP inoculation, bacteria recovered from the spleen) and pneumonia at 48 hours (IN inoculation, bacteria recovered from the lungs). Each point represents the CI for a single animal. (B) Time course of the development of fatal infection for groups of 10 mice inoculated IP with 3×10<sup>3</sup> CFU of the wild-type (WT) and Δ<i>lgt</i> strains (<i>P</i><0.0001, log rank test). (C) Log<sub>10</sub> bacterial CFU per ml of nasal wash recovered 1, 2, 5 and 10 days after nasopharyngeal colonisation of mice with 5×10<sup>6</sup> CFU of the wild-type (black columns) and Δ<i>lgt</i> D39 (clear columns) strains. <i>P</i> values were calculated using Mann Whitney U tests for each time point.</p>", "links"=>[], "tags"=>["mutant"], "article_id"=>274945, "categories"=>["Biochemistry", "Microbiology"], "users"=>["Suneeta Chimalapati", "Jonathan M. Cohen", "Emilie Camberlein", "Nathanael MacDonald", "Claire Durmort", "Thierry Vernet", "Peter W. M. Hermans", "Timothy Mitchell", "Jeremy S. Brown"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041393.g010", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Virulence_of_the_lgt_mutant_strain_/274945", "title"=>"Virulence of the Δ<i>lgt</i> mutant strain.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 02:02:30"}

PMC Usage Stats | Further Information

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