Novel Cell and Tissue Acquisition System (CTAS): Microdissection of Live and Frozen Brain Tissues
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{"title"=>"Novel cell and tissue acquisition system (CTAS): Microdissection of live and frozen brain tissues", "type"=>"journal", "authors"=>[{"first_name"=>"Lili C.", "last_name"=>"Kudo", "scopus_author_id"=>"6701450712"}, {"first_name"=>"Nancy", "last_name"=>"Vi", "scopus_author_id"=>"36553573000"}, {"first_name"=>"Zhongcai", "last_name"=>"Ma", "scopus_author_id"=>"21233838700"}, {"first_name"=>"Tony", "last_name"=>"Fields", "scopus_author_id"=>"7004423668"}, {"first_name"=>"Nuraly K.", "last_name"=>"Avliyakulov", "scopus_author_id"=>"6506679166"}, {"first_name"=>"Michael J.", "last_name"=>"Haykinson", "scopus_author_id"=>"6507016967"}, {"first_name"=>"Anatol", "last_name"=>"Bragin", "scopus_author_id"=>"35271124200"}, {"first_name"=>"Stanislav L.", "last_name"=>"Karsten", "scopus_author_id"=>"6701736036"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84864266958", "sgr"=>"84864266958", "pui"=>"365316815", "isbn"=>"1932-6203", "pmid"=>"22855692", "doi"=>"10.1371/journal.pone.0041564"}, "id"=>"006039b4-653a-35f1-8c0e-ba7ecfda52e6", "abstract"=>"We developed a novel, highly accurate, capillary based vacuum-assisted microdissection device CTAS-Cell and Tissue Acquisition System, for efficient isolation of enriched cell populations from live and freshly frozen tissues, which can be successfully used in a variety of molecular studies, including genomics and proteomics. Specific diameter of the disposable capillary unit (DCU) and precisely regulated short vacuum impulse ensure collection of the desired tissue regions and even individual cells. We demonstrated that CTAS is capable of dissecting specific regions of live and frozen mouse and rat brain tissues at the cellular resolution with high accuracy. CTAS based microdissection avoids potentially harmful physical treatment of tissues such as chemical treatment, laser irradiation, excessive heat or mechanical cell damage, thus preserving primary functions and activities of the dissected cells and tissues. High quality DNA, RNA, and protein can be isolated from CTAS-dissected samples, which are suitable for sequencing, microarray, 2D gel-based proteomic analyses, and Western blotting. We also demonstrated that CTAS can be used to isolate cells from native living tissues for subsequent recultivation of primary cultures without affecting cellular viability, making it a simple and cost-effective alternative for laser-assisted microdissection.", "link"=>"http://www.mendeley.com/research/novel-cell-tissue-acquisition-system-ctas-microdissection-live-frozen-brain-tissues", "reader_count"=>33, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Researcher"=>10, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>1, "Other"=>4, "Student > Master"=>3, "Student > Bachelor"=>4, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Researcher"=>10, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>1, "Other"=>4, "Student > Master"=>3, "Student > Bachelor"=>4, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>3, "Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>4, "Medicine and Dentistry"=>5, "Agricultural and Biological Sciences"=>15, "Neuroscience"=>4}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>3}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>5}, "Neuroscience"=>{"Neuroscience"=>4}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>15}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"Hong Kong"=>1, "United States"=>3, "United Kingdom"=>1, "Germany"=>1}, "group_count"=>2}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/604049"], "description"=>"<p><b>A.</b> DCU consists of a Luer hub, connected to a glass capillary with an adhesive. <b>B.</b> The tip of the capillary is illuminated by the reflected light from the orthogonal light source (1) directed onto a treated or coated surface of the glass capillary barrel (2). To enhance this effect, additional light source (3) maybe used above the upper capillary opening to produce better visualization of the low capillary tip end. Lower images shows the actual microscope visualization of the capillary tip (OD = 20 µm). <b>C.</b> Calibration procedure involves three DCU positions: starting position prior to calibration, home position when the DCU tip is in contact with tissue section and standby position when the DCU tip is lifted above the tissue sample and ready to perform multiple acquisitions; <b>D, E.</b> Tissue sample in the barrel of the capillary. OD = 50 µm.</p>", "links"=>[], "tags"=>["capillary", "positions", "calibration"], "article_id"=>274532, "categories"=>["Biotechnology", "Biochemistry", "Neuroscience", "Genetics", "Developmental Biology"], "users"=>["Lili C. Kudo", "Nancy Vi", "Zhongcai Ma", "Tony Fields", "Nuraly K. Avliyakulov", "Michael J. Haykinson", "Anatol Bragin", "Stanislav L. Karsten"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041564.g002", "stats"=>{"downloads"=>1, "page_views"=>38, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Disposable_capillary_unit_DCU_and_its_positions_during_calibration_and_sample_collection_/274532", "title"=>"Disposable capillary unit (DCU) and its positions during calibration and sample collection.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-07-24 01:15:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/605022"], "description"=>"<p>Representative quality of total RNA isolated from sucrose treated (1–4) and fresh frozen (5–6) mouse (1–2, 5–6) and rat (3–4) brain tissue samples. RNA integrity numbers (RIN) are shown for each sample. <b>B.</b> Mean RNA integrity numbers (RIN; n≥3) over dissection time show slow decline over time but remain within acceptable range for further analysis. Only 120–130 minutes time point demonstrate significant difference from RNA isolated immediately. <b>C.</b> Representative quality of total RNA isolated from brain tissues (mouse cortex) at different time points. <b>D.</b> Representative RNA isolated from liver and kidney tissues using CTAS based procedure demonstrates acceptable RNA quality after 30 minutes (liver) and 60 minutes (kidney) of microdissection.</p>", "links"=>[], "tags"=>["rna", "ctas", "microdissected", "samples", "shows"], "article_id"=>275493, "categories"=>["Biotechnology", "Biochemistry", "Neuroscience", "Genetics", "Developmental Biology"], "users"=>["Lili C. Kudo", "Nancy Vi", "Zhongcai Ma", "Tony Fields", "Nuraly K. Avliyakulov", "Michael J. Haykinson", "Anatol Bragin", "Stanislav L. Karsten"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041564.g007", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Total_RNA_isolated_from_CTAS_microdissected_samples_shows_good_integrity_A_/275493", "title"=>"Total RNA isolated from CTAS microdissected samples shows good integrity. A.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-07-24 01:31:33"}
  • {"files"=>["https://ndownloader.figshare.com/files/605382"], "description"=>"<p>Representative images of subgranular layer of dentate gyrus collection from native adult rat brain tissue before (<b>A</b>), during (<b>B</b>) and after dissection (<b>C</b>). Tissue thickness  = 300 µm, Scale bar  = 250 µm; <b>D.</b> Phase contrast images of neurosphere colonies derived from E18 rat subventricular zone (SVZ). Partially differentiated (<b>E</b>) and undifferentiated (lower right inset) neurosphere (NS) colonies immunostained for nestin (green) and GFAP (red), the marker of neural progenitors and astrocytes, respectively. Scale bar  = 100 µm.</p>", "links"=>[], "tags"=>["neurogenic", "zones", "tissues", "neural", "progenitor", "cultures"], "article_id"=>275846, "categories"=>["Biotechnology", "Biochemistry", "Neuroscience", "Genetics", "Developmental Biology"], "users"=>["Lili C. Kudo", "Nancy Vi", "Zhongcai Ma", "Tony Fields", "Nuraly K. Avliyakulov", "Michael J. Haykinson", "Anatol Bragin", "Stanislav L. Karsten"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041564.g009", "stats"=>{"downloads"=>1, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Collection_of_the_neurogenic_zones_from_native_mouse_brain_tissues_and_neural_progenitor_cultures_using_CTAS_live_A_8211_C_/275846", "title"=>"Collection of the neurogenic zones from native mouse brain tissues and neural progenitor cultures using CTAS-live. A–C.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-07-24 01:37:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/604709"], "description"=>"<p>–<b>D) and Purkinje cells (E</b>–<b>F) from sucrose treated mouse coronal brain sections.</b> Images before (<b>A</b>, <b>C</b>, <b>E</b>) and after (<b>B</b>, <b>D</b>, <b>F</b>) collection are shown. Red arrows and dashed circles show collected cells. Tissue thickness  = 20 µm. DCU ID = 20 µm. Magnification: 400X.</p>", "links"=>[], "tags"=>["ca1", "interneurons", "stratum", "orience"], "article_id"=>275185, "categories"=>["Biotechnology", "Biochemistry", "Neuroscience", "Genetics", "Developmental Biology"], "users"=>["Lili C. Kudo", "Nancy Vi", "Zhongcai Ma", "Tony Fields", "Nuraly K. Avliyakulov", "Michael J. Haykinson", "Anatol Bragin", "Stanislav L. Karsten"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041564.g005", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Representative_collection_of_individual_CA1_interneurons_in_stratum_orience_A_/275185", "title"=>"Representative collection of individual CA1 interneurons in stratum orience (A", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-07-24 01:26:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/604528"], "description"=>"<p>Red dotted line outlines the area of interest. <b>B.</b> Dissection of granular cells from the middle part of the granular layer. <b>C.</b> Additional dissection from the same area. Abbreviations: Sf – the secondary fissure of the cerebellum; 9Cb –9<sup>th</sup> area of cerebellum, Ecu – external cuneate nucleus <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041564#pone.0041564-Paxinos1\" target=\"_blank\">[50]</a>.</p>", "links"=>[], "tags"=>["microdissection", "granular", "cells", "lobe", "cerebellum", "unstained", "moue", "sections"], "article_id"=>274999, "categories"=>["Biotechnology", "Biochemistry", "Neuroscience", "Genetics", "Developmental Biology"], "users"=>["Lili C. Kudo", "Nancy Vi", "Zhongcai Ma", "Tony Fields", "Nuraly K. Avliyakulov", "Michael J. Haykinson", "Anatol Bragin", "Stanislav L. Karsten"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041564.g004", "stats"=>{"downloads"=>1, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sequential_microdissection_of_granular_cells_from_the_9_th_lobe_of_cerebellum_using_fresh_frozen_and_unstained_moue_brain_sections_thickness_8202_8202_20_181_m_A_/274999", "title"=>"Sequential microdissection of granular cells from the 9<sup>th</sup> lobe of cerebellum using fresh frozen and unstained moue brain sections (thickness  = 20 µm). A.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-07-24 01:23:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/604292"], "description"=>"<p>Collection of the left hilus of the dentate gyrus (hDG). <b>B.</b> Dissection of granular cells (G), molecular layer (M), and white matter (W) from mouse cerebellum. Intact (<b>C</b>) and dissected (<b>D</b>) anterior commissure, anterior (ACA) and right piriform cortex (Rpir). <b>E.</b> Dissected (left) and intact (right) thalamic and hypothalamic areas including posterior thalamic nucleus (1), part of ventral posteromedial thalamic nucleus (2), ventromedial thalamic nucleus (3), dorsomedial hypothalamic nucleus (4) and arcuate hypothalamic nucleus (5). Homotopical intact areas are outlined with dashed red lines. Tissues were stained with Toluidine Blue. Scale bar  = 250 µm. DCU ID = 50 µm; vacuum pulse duration: 100 ms; <b>F.</b> Representative microdissection of middle molecular layer of the dentate gyrus (a) and cellular layer of the subiculum (b) from fresh frozen nonstained mouse brain sections (20 µm thickness). Abbreviations: CA1–CA1 area of hippocampus; Sub – subiculum; DG – dentate gyrus; hf – hippocampal fissure. Scale bar  = 100 µm.</p>", "links"=>[], "tags"=>["subanatomical", "regions", "dotted", "coronal", "sections", "ctas"], "article_id"=>274767, "categories"=>["Biotechnology", "Biochemistry", "Neuroscience", "Genetics", "Developmental Biology"], "users"=>["Lili C. Kudo", "Nancy Vi", "Zhongcai Ma", "Tony Fields", "Nuraly K. Avliyakulov", "Michael J. Haykinson", "Anatol Bragin", "Stanislav L. Karsten"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041564.g003", "stats"=>{"downloads"=>1, "page_views"=>24, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Dissection_of_subanatomical_regions_marked_with_red_dotted_line_or_arrows_from_fresh_frozen_mouse_coronal_brain_sections_20_181_m_thickness_using_CTAS_v_4_1_A_/274767", "title"=>"Dissection of subanatomical regions (marked with red dotted line or arrows) from fresh frozen mouse coronal brain sections (20 µm thickness) using CTAS v.4.1. A.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-07-24 01:19:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/604929"], "description"=>"<p>Adult mouse coronal neocortical tissue sections were used. As expected, increased vacuum strength and duration, as well as thicker tissues, negatively affect the accuracy of the dissection producing larger acquisition areas with higher variability. At least 5 individual acquisitions (n≥5) were made to estimate mean acquisition area for each parameter tested. Error bars represent standard deviations; <b>A.</b> Mean values of acquisition area for 20 µm tissue and 20 µm DCU ID. Notice the proportional increase in the average area with the increase in the vacuum strength; <b>B.</b> Longer vacuum duration produces larger acquisition area. Tissue thickness: 20 µm, DCU ID = 90 µm; <b>C.</b> For the same vacuum strength and duration, thicker tissues produce larger and more variable acquisition areas compared to thinner sections. Comparisons were made for 0.5 sec (left) and 0.1 sec (right) time duration. Tissue thickness 20 µm (light grey) and 50 µm (dark grey), DCU ID = 90 µm.</p>", "links"=>[], "tags"=>["charts", "dependence", "duration"], "article_id"=>275409, "categories"=>["Biotechnology", "Biochemistry", "Neuroscience", "Genetics", "Developmental Biology"], "users"=>["Lili C. Kudo", "Nancy Vi", "Zhongcai Ma", "Tony Fields", "Nuraly K. Avliyakulov", "Michael J. Haykinson", "Anatol Bragin", "Stanislav L. Karsten"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041564.g006", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Representative_charts_demonstrate_the_dependence_of_average_acquisition_area_on_the_vacuum_strength_vacuum_duration_and_tissue_thickness_/275409", "title"=>"Representative charts demonstrate the dependence of average acquisition area on the vacuum strength, vacuum duration and tissue thickness.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-07-24 01:30:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/603877"], "description"=>"<p><b>A.</b> Representative image of CTAS v.4.1. The system is attached to an inverted microscope (TCM400, Labomed) and consists of the following key components: sample collection assembly with collection unit, LED lights, side chassis vacuum module and DCU controls. The latter incorporates electronic controls and a vacuum pump. The two dials at the front of the side chassis control the vacuum strength from 2″Hg (1) to maximum of 22″Hg (10) and the vacuum duration from 100 ms (1) to maximum of 1 second (10). Depending on the tissue type and section thickness, various vacuum strength and duration may be used. Green button turns the power on/off. Three DCU control buttons include two white buttons, which bring the DCU up or down during the calibration procedure and an orange button that sets the Home position of the DCU and brings it to its Standby position. Black “Sample” button initiates sample collection by bringing the DCU down to the Home position and activating the vacuum at the selected strength and duration; <b>B.</b> CTAS sample collection assembly in its lifted position for DCU attachment/removal. DCU attached to a collection unit with connectors for multiple cables and a vacuum tube. Calibration LED source for illuminating the tip of the capillary is shown. In this position, the green horizontal and red vertical calibration LED lights are automatically turned off. The lights are automatically turned on when the CTAS head is in its upright position. The x–y position of the DCU is controlled by the knobs on the linear stages (x–y controls).</p>", "links"=>[], "tags"=>["vacuum-assisted"], "article_id"=>274354, "categories"=>["Biotechnology", "Biochemistry", "Neuroscience", "Genetics", "Developmental Biology"], "users"=>["Lili C. Kudo", "Nancy Vi", "Zhongcai Ma", "Tony Fields", "Nuraly K. Avliyakulov", "Michael J. Haykinson", "Anatol Bragin", "Stanislav L. Karsten"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041564.g001", "stats"=>{"downloads"=>1, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Capillary_based_vacuum_assisted_cell_and_tissue_acquisition_system_CTAS_v_4_1_/274354", "title"=>"Capillary-based vacuum-assisted cell and tissue acquisition system (CTAS) v.4.1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-07-24 01:12:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/605618"], "description"=>"<p>Estimation of mean acquisition areas for various DCU IDs and tissue thicknesses using neocortical sucrose treated mouse brain sections.</p>", "links"=>[], "tags"=>["areas", "dcu", "ids", "thicknesses", "neocortical", "sucrose", "treated"], "article_id"=>276081, "categories"=>["Biotechnology", "Biochemistry", "Neuroscience", "Genetics", "Developmental Biology"], "users"=>["Lili C. Kudo", "Nancy Vi", "Zhongcai Ma", "Tony Fields", "Nuraly K. Avliyakulov", "Michael J. Haykinson", "Anatol Bragin", "Stanislav L. Karsten"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041564.t001", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Estimation_of_mean_acquisition_areas_for_various_DCU_IDs_and_tissue_thicknesses_using_neocortical_sucrose_treated_mouse_brain_sections_/276081", "title"=>"Estimation of mean acquisition areas for various DCU IDs and tissue thicknesses using neocortical sucrose treated mouse brain sections.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-07-24 01:41:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/605130"], "description"=>"<p>Representative 2D gel electrophoresis analysis of fresh frozen CA2–CA3 and DG areas of hippocampus (stained by Sypro Ruby) is shown. Protein samples from CA2–CA3 and DG areas were labeled with Cy3 and Cy5 correspondingly, imaged on the Typhoon, and analyzed using DIA module of Decyder. Gel was fixed and stained with Sypro Ruby; protein spots were picked and identified using MS. The following differences in protein abundances between identified proteins isolated by CTAS from CA2–CA3 and DG areas were observed (CA2–CA3/DG abundances ratios are shown in parenthesis): HS90B (−1.52), TBB4B (−2.34), PP2AB (−1.55), PGP (used as control, no difference), SPEE (−1.59), LDHB (used as control, no difference), CALB2 (1.76), NCALD (1.77), PROF1 (1.65).</p>", "links"=>[], "tags"=>["ctas-dissected", "samples", "downstream", "applications", "2d", "gel"], "article_id"=>275608, "categories"=>["Biotechnology", "Biochemistry", "Neuroscience", "Genetics", "Developmental Biology"], "users"=>["Lili C. Kudo", "Nancy Vi", "Zhongcai Ma", "Tony Fields", "Nuraly K. Avliyakulov", "Michael J. Haykinson", "Anatol Bragin", "Stanislav L. Karsten"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0041564.g008", "stats"=>{"downloads"=>3, "page_views"=>41, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Proteins_isolated_from_CTAS_dissected_tissue_samples_preserve_high_integrity_and_may_be_used_for_downstream_applications_including_2D_gel_electrophoresis_/275608", "title"=>"Proteins isolated from CTAS-dissected tissue samples preserve high integrity and may be used for downstream applications including 2D gel electrophoresis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-07-24 01:33:28"}
  • {"files"=>["https://ndownloader.figshare.com/files/315990", "https://ndownloader.figshare.com/files/316040"], "description"=>"<div><p>We developed a novel, highly accurate, capillary based vacuum-assisted microdissection device CTAS - Cell and Tissue Acquisition System, for efficient isolation of enriched cell populations from live and freshly frozen tissues, which can be successfully used in a variety of molecular studies, including genomics and proteomics. Specific diameter of the disposable capillary unit (DCU) and precisely regulated short vacuum impulse ensure collection of the desired tissue regions and even individual cells. We demonstrated that CTAS is capable of dissecting specific regions of live and frozen mouse and rat brain tissues at the cellular resolution with high accuracy. CTAS based microdissection avoids potentially harmful physical treatment of tissues such as chemical treatment, laser irradiation, excessive heat or mechanical cell damage, thus preserving primary functions and activities of the dissected cells and tissues. High quality DNA, RNA, and protein can be isolated from CTAS-dissected samples, which are suitable for sequencing, microarray, 2D gel-based proteomic analyses, and Western blotting. We also demonstrated that CTAS can be used to isolate cells from native living tissues for subsequent recultivation of primary cultures without affecting cellular viability, making it a simple and cost-effective alternative for laser-assisted microdissection.</p> </div>", "links"=>[], "tags"=>["microdissection", "tissues"], "article_id"=>122366, "categories"=>["Biotechnology", "Biochemistry", "Neuroscience", "Genetics", "Developmental Biology"], "users"=>["Lili C. Kudo", "Nancy Vi", "Zhongcai Ma", "Tony Fields", "Nuraly K. Avliyakulov", "Michael J. Haykinson", "Anatol Bragin", "Stanislav L. Karsten"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0041564.s001", "https://dx.doi.org/10.1371/journal.pone.0041564.s002"], "stats"=>{"downloads"=>14, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Novel_Cell_and_Tissue_Acquisition_System_CTAS_Microdissection_of_Live_and_Frozen_Brain_Tissues/122366", "title"=>"Novel Cell and Tissue Acquisition System (CTAS): Microdissection of Live and Frozen Brain Tissues", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-07-24 00:39:26"}

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