Cathepsin K-Cre Causes Unexpected Germline Deletion of Genes in Mice
Publication Date
July 31, 2012
Journal
PLOS ONE
Authors
Crystal L. Winkeler, Raleigh D. Kladney, Leonard B. Maggi Jr & Jason D. Weber
Volume
7
Issue
7
Pages
e42005
DOI
https://dx.plos.org/10.1371/journal.pone.0042005
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0042005
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/22860046
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3409209
Europe PMC
http://europepmc.org/abstract/MED/22860046
Web of Science
000307045600050
Scopus
84864451590
Mendeley
http://www.mendeley.com/research/cathepsin-kcre-causes-unexpected-germline-deletion-genes-mice
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Mendeley | Further Information

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Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/599156"], "description"=>"<p>(A) Three sets of primer pairs were designed to detect the presence of floxed <i>exon 1beta</i>, which is unique to <i>Arf.</i> (B) PCR products were not detected upon genotyping new mice for the presence of the 5′ loxP site (far left panel). This cannot be attributed to an absence of DNA (middle left panel). Controls are mice that were never crossed with <i>Ctsk<sup>Cre/+</sup></i> mice. Two sets of primer pairs were used to detect the presence of <i>exon 1beta</i>. Each set detects a product indicating loss of <i>exon 1beta</i> (product size labeled as “KO” in table). (C) Immunofluorescent staining for ARF in testis tissues indicates the loss of <i>exon 1beta</i> at the protein level (scale bar = 100 µM).</p>", "links"=>[], "tags"=>["mice", "germline"], "article_id"=>269648, "categories"=>["Biochemistry", "Cell Biology", "Genetics", "Mathematics"], "users"=>["Crystal L. Winkeler", "Raleigh D. Kladney", "Leonard B. Maggi Jr", "Jason D. Weber"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0042005.g002", "stats"=>{"downloads"=>2, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Crossing_Arf_fl_fl_mice_with_CtsK_Cre_mice_results_in_germline_Arf_loss_/269648", "title"=>"Crossing <i>Arf<sup> fl/fl</sup></i> mice with <i>CtsK<sup>Cre/+</sup></i> mice results in germline <i>Arf</i> loss.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 01:32:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/599240"], "description"=>"<p>(A) Real-time PCR was used to quantify the presence of Cathepsin K mRNA (blue) and Cre mRNA (red) in ovary (left) and testis (right) for all indicated genotypes (n = 3). Data are represented as means ± SD. A two-tailed t-test was used to generate indicated p values. (B) Ovaries from WT mice analyzed by IHC for Cathepsin K. Top panels, scale bar = 200 µM. Bottom, left panel shows staining without primary antibody. An adjacent section to the control (bottom, middle) was incubated with primary antibody (scale bar = 100 µM). Red box indicates positively-stained oocyte (bottom, right scale bar = 20 µM).</p>", "links"=>[], "tags"=>["gametes"], "article_id"=>269734, "categories"=>["Biochemistry", "Cell Biology", "Genetics", "Mathematics"], "users"=>["Crystal L. Winkeler", "Raleigh D. Kladney", "Leonard B. Maggi Jr", "Jason D. Weber"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0042005.g003", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cre_is_expressed_in_the_gametes_of_Ctsk_Cre_mice_/269734", "title"=>"Cre is expressed in the gametes of <i>Ctsk<sup>Cre/+</sup></i> mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 01:33:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/598984"], "description"=>"<p>(A) <i>Ctsk<sup>Cre/+</sup></i>; <i>Arf<sup>fl/fl</sup></i> mice display both fibrosarcomas (top image) and lymphomas with splenomegaly (middle and bottom images). (B) Spontaneous tumor development occurred in all <i>Ctsk<sup>Cre/+</sup></i>; <i>Arf<sup>fl/fl</sup></i> mice.</p>", "links"=>[], "tags"=>["mice", "spontaneous"], "article_id"=>269476, "categories"=>["Biochemistry", "Cell Biology", "Genetics", "Mathematics"], "users"=>["Crystal L. Winkeler", "Raleigh D. Kladney", "Leonard B. Maggi Jr", "Jason D. Weber"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0042005.g001", "stats"=>{"downloads"=>0, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Crossing_Arf_fl_fl_mice_with_CtsK_Cre_mice_results_in_spontaneous_tumor_formation_/269476", "title"=>"Crossing <i>Arf<sup>fl/fl</sup></i> mice with <i>CtsK<sup>Cre/+</sup></i> mice results in spontaneous tumor formation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 01:31:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/599372"], "description"=>"<p>Reproductive organs of indicated genotypes were analyzed for Cre activity by LacZ staining. (A) In ovaries of <i>Rosa<sup>+</sup></i>; <i>Ctsk<sup>Cre/+</sup></i> mice, Cre activity was detected in oocytes and cells surrounding the developing oocytes (left panel). Controls were negative for LacZ staining (right panel). Scale bar = 50 µM. (B) In testes of <i>Rosa<sup>+</sup></i>; <i>Ctsk<sup>Cre/+</sup></i> mice, Cre activity was detected primarily in spermatozoa (left panel). Controls were negative for LacZ staining (right panel). Scale bar = 100 µM.</p>", "links"=>[], "tags"=>["gametes", "verified"], "article_id"=>269871, "categories"=>["Biochemistry", "Cell Biology", "Genetics", "Mathematics"], "users"=>["Crystal L. Winkeler", "Raleigh D. Kladney", "Leonard B. Maggi Jr", "Jason D. Weber"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0042005.g004", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cre_expression_in_Ctsk_Cre_mouse_gametes_is_verified_with_Rosa_mice_/269871", "title"=>"Cre expression in <i>Ctsk<sup>Cre/+</sup></i> mouse gametes is verified with <i>Rosa<sup>+</sup></i> mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 01:33:48"}

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