A SINE-Derived Element Constitutes a Unique Modular Enhancer for Mammalian Diencephalic Fgf8
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"A sine-derived element constitutes a unique modular enhancer for mammalian diencephalic fgf8", "type"=>"journal", "authors"=>[{"first_name"=>"Akiko", "last_name"=>"Nakanishi", "scopus_author_id"=>"54420804400"}, {"first_name"=>"Naoki", "last_name"=>"Kobayashi", "scopus_author_id"=>"55587041100"}, {"first_name"=>"Asuka", "last_name"=>"Suzuki-Hirano", "scopus_author_id"=>"6504074314"}, {"first_name"=>"Hidenori", "last_name"=>"Nishihara", "scopus_author_id"=>"7202718772"}, {"first_name"=>"Takeshi", "last_name"=>"Sasaki", "scopus_author_id"=>"55619302611"}, {"first_name"=>"Mika", "last_name"=>"Hirakawa", "scopus_author_id"=>"8744752900"}, {"first_name"=>"Kenta", "last_name"=>"Sumiyama", "scopus_author_id"=>"7101935921"}, {"first_name"=>"Tomomi", "last_name"=>"Shimogori", "scopus_author_id"=>"6506799075"}, {"first_name"=>"Norihiro", "last_name"=>"Okada", "scopus_author_id"=>"7201737336"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"isbn"=>"1932-6203 (Electronic)\\n1932-6203 (Linking)", "pui"=>"365489788", "doi"=>"10.1371/journal.pone.0043785", "scopus"=>"2-s2.0-84865286053", "sgr"=>"84865286053", "issn"=>"19326203", "pmid"=>"22937095"}, "id"=>"fd28c9e0-ec1f-3bda-a333-eaf4a25bd5a1", "abstract"=>"Transposable elements, including short interspersed repetitive elements (SINEs), comprise nearly half the mammalian genome. Moreover, they are a major source of conserved non-coding elements (CNEs), which play important functional roles in regulating development-related genes, such as enhancing and silencing, serving for the diversification of morphological and physiological features among species. We previously reported a novel SINE family, AmnSINE1, as part of mammalian-specific CNEs. One AmnSINE1 locus, named AS071, showed an enhancer property in the developing mouse diencephalon. Indeed, AS071 appears to recapitulate the expression of diencephalic fibroblast growth factor 8 (Fgf8). Here we established three independent lines of AS071-transgenic mice and performed detailed expression profiling of AS071-enhanced lacZ in comparison with that of Fgf8 across embryonic stages. We demonstrate that AS071 is a distal enhancer that directs Fgf8 expression in the developing diencephalon. Furthermore, enhancer assays with constructs encoding partially deleted AS071 sequence revealed a unique modular organization in which AS071 contains at least three functionally distinct sub-elements that cooperatively direct the enhancer activity in three diencephalic domains, namely the dorsal midline and the lateral wall of the diencephalon, and the ventral midline of the hypothalamus. Interestingly, the AmnSINE1-derived sub-element was found to specify the enhancer activity to the ventral midline of the hypothalamus. To our knowledge, this is the first discovery of an enhancer element that could be separated into respective sub-elements that determine regional specificity and/or the core enhancing activity. These results potentiate our understanding of the evolution of retroposon-derived cis-regulatory elements as well as the basis for future studies of the molecular mechanism underlying the determination of domain-specificity of an enhancer.", "link"=>"http://www.mendeley.com/research/sinederived-element-constitutes-unique-modular-enhancer-mammalian-diencephalic-fgf8", "reader_count"=>27, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Researcher"=>8, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>2, "Student > Master"=>4, "Student > Bachelor"=>2, "Professor"=>1, "Unspecified"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Researcher"=>8, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>2, "Student > Master"=>4, "Student > Bachelor"=>2, "Professor"=>1, "Unspecified"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>21, "Medicine and Dentistry"=>1, "Computer Science"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>21}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"Canada"=>1, "Brazil"=>3, "United Kingdom"=>1, "Spain"=>1}, "group_count"=>1}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84865286053"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84865286053?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84865286053&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84865286053&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84865286053", "dc:identifier"=>"SCOPUS_ID:84865286053", "eid"=>"2-s2.0-84865286053", "dc:title"=>"A sine-derived element constitutes a unique modular enhancer for mammalian diencephalic fgf8", "dc:creator"=>"Nakanishi A.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"7", "prism:issueIdentifier"=>"8", "prism:pageRange"=>nil, "prism:coverDate"=>"2012-08-24", "prism:coverDisplayDate"=>"24 August 2012", "prism:doi"=>"10.1371/journal.pone.0043785", "citedby-count"=>"21", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Tokyo Institute of Technology", "affiliation-city"=>"Tokyo", "affiliation-country"=>"Japan"}], "pubmed-id"=>"22937095", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e43785", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0043785", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"97"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"42", "xml_views"=>"1", "html_views"=>"103"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"81"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"84"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"71"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"39"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"53"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"3", "html_views"=>"23"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"34"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"67"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"56"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"4", "html_views"=>"37"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"42"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"15"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"38"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"44"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"3", "html_views"=>"4"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"3", "html_views"=>"10"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"5"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/308872", "https://ndownloader.figshare.com/files/308943", "https://ndownloader.figshare.com/files/309124", "https://ndownloader.figshare.com/files/309219", "https://ndownloader.figshare.com/files/309275", "https://ndownloader.figshare.com/files/309331", "https://ndownloader.figshare.com/files/309373"], "description"=>"<div><p>Transposable elements, including short interspersed repetitive elements (SINEs), comprise nearly half the mammalian genome. Moreover, they are a major source of conserved non-coding elements (CNEs), which play important functional roles in regulating development-related genes, such as enhancing and silencing, serving for the diversification of morphological and physiological features among species. We previously reported a novel SINE family, AmnSINE1, as part of mammalian-specific CNEs. One AmnSINE1 locus, named AS071, showed an enhancer property in the developing mouse diencephalon. Indeed, AS071 appears to recapitulate the expression of diencephalic fibroblast growth factor 8 (<em>Fgf8</em>). Here we established three independent lines of AS071-transgenic mice and performed detailed expression profiling of AS071-enhanced <em>lacZ</em> in comparison with that of <em>Fgf8</em> across embryonic stages. We demonstrate that AS071 is a distal enhancer that directs <em>Fgf8</em> expression in the developing diencephalon. Furthermore, enhancer assays with constructs encoding partially deleted AS071 sequence revealed a unique modular organization in which AS071 contains at least three functionally distinct sub-elements that cooperatively direct the enhancer activity in three diencephalic domains, namely the dorsal midline and the lateral wall of the diencephalon, and the ventral midline of the hypothalamus. Interestingly, the AmnSINE1-derived sub-element was found to specify the enhancer activity to the ventral midline of the hypothalamus. To our knowledge, this is the first discovery of an enhancer element that could be separated into respective sub-elements that determine regional specificity and/or the core enhancing activity. These results potentiate our understanding of the evolution of retroposon-derived <em>cis</em>-regulatory elements as well as the basis for future studies of the molecular mechanism underlying the determination of domain-specificity of an enhancer.</p> </div>", "links"=>[], "tags"=>["sine-derived", "constitutes", "modular", "enhancer", "mammalian", "diencephalic"], "article_id"=>120951, "categories"=>["Genetics", "Developmental Biology", "Evolutionary Biology"], "users"=>["Akiko Nakanishi", "Naoki Kobayashi", "Asuka Suzuki-Hirano", "Hidenori Nishihara", "Takeshi Sasaki", "Mika Hirakawa", "Kenta Sumiyama", "Tomomi Shimogori", "Norihiro Okada"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0043785.s001", "https://dx.doi.org/10.1371/journal.pone.0043785.s002", "https://dx.doi.org/10.1371/journal.pone.0043785.s003", "https://dx.doi.org/10.1371/journal.pone.0043785.s004", "https://dx.doi.org/10.1371/journal.pone.0043785.s005", "https://dx.doi.org/10.1371/journal.pone.0043785.s006", "https://dx.doi.org/10.1371/journal.pone.0043785.s007"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/A_SINE_Derived_Element_Constitutes_a_Unique_Modular_Enhancer_for_Mammalian_Diencephalic_Fgf8_/120951", "title"=>"A SINE-Derived Element Constitutes a Unique Modular Enhancer for Mammalian Diencephalic <em>Fgf8</em>", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-08-24 00:15:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/587248"], "description"=>"<p>(A) The human AS071 (chr10∶103,356,749–103,357,337, GRCh37/hg19) in the UCSC Genome Browser (<a href=\"http://genome.ucsc.edu/\" target=\"_blank\">http://genome.ucsc.edu/</a>). The red bar corresponds to the AmnSINE1-derived region (homologous to the AmnSINE1 consensus sequence, 204 bp), and the black bar shows the region conserved among mammals (590 bp). Sequence alignment of the region is shown in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0043785#pone.0043785.s001\" target=\"_blank\">Figure S1</a>. (B) The conserved gene synteny of the 1-Mb region around the locus among human (chr10∶103,000,000–104,000,000, GRCh37/hg19), mouse (chr19∶45,310,000–46,310,000, NCBI37/mm9), dog (chr28∶16,900,000–17,900,000, Broad/canFam2), and opossum (chr1∶110,001,000–111,001,000, Broad/monDom5). Note the inversion in this region in opossum. Gene annotations are based on the human assembly in the UCSC Genome Browser.</p>", "links"=>[], "tags"=>["synteny"], "article_id"=>257739, "categories"=>["Genetics", "Developmental Biology", "Evolutionary Biology"], "users"=>["Akiko Nakanishi", "Naoki Kobayashi", "Asuka Suzuki-Hirano", "Hidenori Nishihara", "Takeshi Sasaki", "Mika Hirakawa", "Kenta Sumiyama", "Tomomi Shimogori", "Norihiro Okada"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0043785.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Conservation_of_the_sequence_and_surrounding_gene_synteny_of_AS071_/257739", "title"=>"Conservation of the sequence and surrounding gene synteny of AS071.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-08-24 02:08:59"}
  • {"files"=>["https://ndownloader.figshare.com/files/587387"], "description"=>"<p>(A) <i>LacZ</i> expression pattern in developing mouse diencephalon directed by AS071 in the transgenic Line C. X-Gal staining for β-galactosidase activity in AS071-<i>lacZ</i> transgenic mouse embryos through E9.5–E15.5 shows a dynamic pattern of <i>lacZ</i> expression in the diencephalic domain. An ectopic expression in midbrain peculiar to this line was observed at E15.5. (a–g) Dorso-frontal views of whole-mount stained dissected brains. (a’–g’) Lateral views. The left telencephalon was removed. (a”–g”, e”’–g”’) Sagittal sections. The expression profiling of all three lines is shown in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0043785#pone.0043785.s002\" target=\"_blank\">Figure S2</a>. Scale bars: 0.5 mm (a–c, a’–c’), 1.0 mm (d–g, d’–g’), 0.4 mm (a”–g”, e”’–g”’). (B) Comparison of spatiotemporal <i>Fgf8</i> expression between mouse and chicken by in situ hybridization. The mouse E10.5, E12.5 and E14.5 stages correspond to chicken HH21, HH23 and HH30, respectively. Colored arrowheads indicate as follows: green, dorsal midline of the diencephalon; blue, lateral wall of the diencephalon; red, ventral midline of the hypothalamus; black, anterior neural ridge; white, optic recess.</p>", "links"=>[], "tags"=>["as071-transgenic", "mice"], "article_id"=>257873, "categories"=>["Genetics", "Developmental Biology", "Evolutionary Biology"], "users"=>["Akiko Nakanishi", "Naoki Kobayashi", "Asuka Suzuki-Hirano", "Hidenori Nishihara", "Takeshi Sasaki", "Mika Hirakawa", "Kenta Sumiyama", "Tomomi Shimogori", "Norihiro Okada"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0043785.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_LacZ_expression_in_AS071_transgenic_mice_and_comparison_of_Fgf8_expression_between_mouse_and_chicken_/257873", "title"=>"<i>LacZ</i> expression in AS071-transgenic mice and comparison of <i>Fgf8</i> expression between mouse and chicken.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-08-24 02:11:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/587544"], "description"=>"<p>(A) Genomic region surrounding the mouse AS071 in the UCSC Genome Browser (chr19∶45,641,837–45,642,422, NCBI37/mm9) and schematic representation of the transgene constructs. AS071 was split into four sub-elements for the deletion analyses: CNE1a, blue box; CNE1b, green box; SINE, red box; CNE2, turquoise box. The fragments with various combinations of AS071 sub-elements were amplified from the AS071-HSF51 construct and re-introduced in the HSF51 vector harboring the mouse heat-shock protein promoter (black box) and bacterial <i>lacZ</i> reporter gene (grey box) followed by the SV40 polyA signal (orange box). Resulting constructs were linearized with <i>Xho</i> I and <i>Not</i> I before microinjection. (B) Results of the enhancer analysis using the AS071-deletion constructs. The letters a–j on the left correspond to those in (C). Schematic diagrams of the organization of each construct are shown. The presence (+) or absence (–) of <i>lacZ</i> expression in each expression domain is indicated. Efficiency denotes the number of embryos showing <i>lacZ</i> expression per those carrying the transgene. (C) (Left) <i>LacZ</i> expressions directed in the diencephalic region by the deletion constructs. Note that all constructs lacking the SINE sub-element (red box) could not direct <i>lacZ</i> expression in the ventral midline of the hypothalamus. Arrowheads indicate as follows: green, dorsal midline of the diencephalon; blue, lateral wall of the diencephalon; red, the ventral midline of the hypothalamus. (Right) The constructs lacking CNE1b (green box) show no enhancer activity in the diencephalon. (D) Summary of the functional dissection of AS071. Whether each AS071-enhancer sub-element showed activity in a particular region is indicated by + or –. The color of each element corresponds to the associated diencephalic domain shown in the illustration. DD, dorsal midline of the diencephalon; LD, lateral wall of the diencephalon; VMH, ventral midline of the hypothalamus.</p>", "links"=>[], "tags"=>["dissection", "as071", "deletion"], "article_id"=>258030, "categories"=>["Genetics", "Developmental Biology", "Evolutionary Biology"], "users"=>["Akiko Nakanishi", "Naoki Kobayashi", "Asuka Suzuki-Hirano", "Hidenori Nishihara", "Takeshi Sasaki", "Mika Hirakawa", "Kenta Sumiyama", "Tomomi Shimogori", "Norihiro Okada"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0043785.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Functional_dissection_of_AS071_using_deletion_constructs_/258030", "title"=>"Functional dissection of AS071 using deletion constructs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-08-24 02:13:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/587641"], "description"=>"<p><i>LacZ</i> expression in AS071-ΔSINE-transgenic embryos shows exactly same pattern as that in AS071-transgenic embryos between E9.5–E14.5 except in the ventral midline of the hypothalamus (red broken-line triangle), indicating the role of the SINE sub-element in determining domain-specificity of the activity to the ventral midline of the hypothalamus. (a–f) Dorso-frontal views of whole-mount stained dissected brains. (a’–f’) Lateral views. The left telencephalon was removed. (a”–f”, e”’–f”’) Sagittal sections. Colored arrowheads represent as follows: green, dorsal midline of the diencephalon; blue, lateral wall of the diencephalon; red, the ventral midline of the hypothalamus. Scale bars: 0.5 mm (a–c, a’–c’), 1.0 mm (d–g, d’–g’), 0.4 mm (a”–g”, e”’–g”’).</p>", "links"=>[], "tags"=>["spatiotemporal", "as071-transgenic"], "article_id"=>258129, "categories"=>["Genetics", "Developmental Biology", "Evolutionary Biology"], "users"=>["Akiko Nakanishi", "Naoki Kobayashi", "Asuka Suzuki-Hirano", "Hidenori Nishihara", "Takeshi Sasaki", "Mika Hirakawa", "Kenta Sumiyama", "Tomomi Shimogori", "Norihiro Okada"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0043785.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparison_of_spatiotemporal_lacZ_expression_pattern_between_the_AS071_SINE_and_AS071_transgenic_lines_/258129", "title"=>"Comparison of spatiotemporal <i>lacZ</i> expression pattern between the AS071-ΔSINE-and AS071-transgenic lines.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-08-24 02:15:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/587741"], "description"=>"<p>Spatiotemporally restricted <i>Fgf8</i> expression in the developing diencephalon by AS071 is accomplished through the interaction of activator proteins that cooperatively bind to each sub-element. The coactivator protein (purple box) mediates the interaction between the activator protein (yellow box), which is ubiquitously expressed in the diencephalon, and other domain-specific activator proteins, as indicated by the colored shapes. Each domain-specific activator protein needs to associate with the core element in CNE1b to effect enhancer activity in each diencephalic domain.</p>", "links"=>[], "tags"=>["domain-specific", "diencephalic"], "article_id"=>258232, "categories"=>["Genetics", "Developmental Biology", "Evolutionary Biology"], "users"=>["Akiko Nakanishi", "Naoki Kobayashi", "Asuka Suzuki-Hirano", "Hidenori Nishihara", "Takeshi Sasaki", "Mika Hirakawa", "Kenta Sumiyama", "Tomomi Shimogori", "Norihiro Okada"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0043785.g005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_A_model_of_domain_specific_expression_of_diencephalic_Fgf8_by_AS071_/258232", "title"=>"A model of domain-specific expression of diencephalic <i>Fgf8</i> by AS071.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-08-24 02:17:12"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"29", "full-text"=>"19", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"19", "full-text"=>"17", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"9", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"22", "full-text"=>"21", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"14", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"4", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"20", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"15", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"7", "cited-by"=>"1", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"1", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"3", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Developmental biology", "average_usage"=>[313, 562, 690, 796, 893, 986, 1079, 1173, 1257, 1349, 1429, 1506, 1586, 1661, 1730, 1803, 1876, 1945, 2017, 2091, 2163, 2225, 2294, 2362, 2430]}, {"subject_area"=>"/Biology and life sciences/Evolutionary biology", "average_usage"=>[366, 574, 695, 795, 885, 973, 1063, 1148, 1233, 1319, 1389, 1459, 1533, 1611, 1685, 1754, 1820, 1882, 1937, 2001, 2071, 2129, 2219, 2273, 2335]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[312, 547, 668, 769, 861, 953, 1046, 1135, 1224, 1307, 1388, 1464, 1536, 1606, 1676, 1744, 1812, 1882, 1954, 2020, 2089, 2155, 2218, 2282, 2344]}, {"subject_area"=>"/Medicine and health sciences/Anatomy", "average_usage"=>[307, 529, 649, 751, 845, 936, 1025, 1113, 1197, 1284, 1360, 1432, 1501, 1570, 1632, 1697, 1765, 1834, 1904, 1972, 2041, 2107, 2170, 2231, 2292]}, {"subject_area"=>"/Medicine and health sciences/Anatomy and physiology", "average_usage"=>[308, 539, 659, 759, 852, 941, 1028, 1113, 1197, 1275, 1353, 1423]}]}
Loading … Spinner
There are currently no alerts