Identification of Thalidomide-Specific Transcriptomics and Proteomics Signatures during Differentiation of Human Embryonic Stem Cells
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Identification of Thalidomide-Specific Transcriptomics and Proteomics Signatures during Differentiation of Human Embryonic Stem Cells", "type"=>"journal", "authors"=>[{"first_name"=>"Kesavan", "last_name"=>"Meganathan", "scopus_author_id"=>"26537857600"}, {"first_name"=>"Smita", "last_name"=>"Jagtap", "scopus_author_id"=>"26537671500"}, {"first_name"=>"Vilas", "last_name"=>"Wagh", "scopus_author_id"=>"26538284500"}, {"first_name"=>"Johannes", "last_name"=>"Winkler", "scopus_author_id"=>"8854082400"}, {"first_name"=>"John Antonydas", "last_name"=>"Gaspar", "scopus_author_id"=>"7006015465"}, {"first_name"=>"Diana", "last_name"=>"Hildebrand", "scopus_author_id"=>"24398858900"}, {"first_name"=>"Maria", "last_name"=>"Trusch", "scopus_author_id"=>"24399848200"}, {"first_name"=>"Karola", "last_name"=>"Lehmann", "scopus_author_id"=>"23028240000"}, {"first_name"=>"Jürgen", "last_name"=>"Hescheler", "scopus_author_id"=>"56213247900"}, {"first_name"=>"Hartmut", "last_name"=>"Schlüter", "scopus_author_id"=>"7006379393"}, {"first_name"=>"Agapios", "last_name"=>"Sachinidis", "scopus_author_id"=>"56273462100"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84865506513", "sgr"=>"84865506513", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0044228", "pmid"=>"22952932", "isbn"=>"1932-6203", "pui"=>"365529032"}, "id"=>"35f7ba90-b08d-3ddb-84e0-2b154585f1dd", "abstract"=>"Embryonic development can be partially recapitulated in vitro by differentiating human embryonic stem cells (hESCs). Thalidomide is a developmental toxicant in vivo and acts in a species-dependent manner. Besides its therapeutic value, thalidomide also serves as a prototypical model to study teratogenecity. Although many in vivo and in vitro platforms have demonstrated its toxicity, only a few test systems accurately reflect human physiology. We used global gene expression and proteomics profiling (two dimensional electrophoresis (2DE) coupled with Tandem Mass spectrometry) to demonstrate hESC differentiation and thalidomide embryotoxicity/teratogenecity with clinically relevant dose(s). Proteome analysis showed loss of POU5F1 regulatory proteins PKM2 and RBM14 and an over expression of proteins involved in neuronal development (such as PAK2, PAFAH1B2 and PAFAH1B3) after 14 days of differentiation. The genomic and proteomic expression pattern demonstrated differential expression of limb, heart and embryonic development related transcription factors and biological processes. Moreover, this study uncovered novel possible mechanisms, such as the inhibition of RANBP1, that participate in the nucleocytoplasmic trafficking of proteins and inhibition of glutathione transferases (GSTA1, GSTA2), that protect the cell from secondary oxidative stress. As a proof of principle, we demonstrated that a combination of transcriptomics and proteomics, along with consistent differentiation of hESCs, enabled the detection of canonical and novel teratogenic intracellular mechanisms of thalidomide.", "link"=>"http://www.mendeley.com/research/identification-thalidomidespecific-transcriptomics-proteomics-signatures-during-differentiation-huma-1", "reader_count"=>49, "reader_count_by_academic_status"=>{"Unspecified"=>3, "Professor > Associate Professor"=>4, "Researcher"=>11, "Student > Doctoral Student"=>5, "Student > Ph. D. Student"=>10, "Student > Postgraduate"=>1, "Student > Master"=>5, "Other"=>2, "Student > Bachelor"=>4, "Professor"=>4}, "reader_count_by_user_role"=>{"Unspecified"=>3, "Professor > Associate Professor"=>4, "Researcher"=>11, "Student > Doctoral Student"=>5, "Student > Ph. D. Student"=>10, "Student > Postgraduate"=>1, "Student > Master"=>5, "Other"=>2, "Student > Bachelor"=>4, "Professor"=>4}, "reader_count_by_subject_area"=>{"Engineering"=>2, "Unspecified"=>6, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>14, "Materials Science"=>1, "Agricultural and Biological Sciences"=>12, "Medicine and Dentistry"=>7, "Pharmacology, Toxicology and Pharmaceutical Science"=>4, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>2}, "Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>7}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>12}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>14}, "Unspecified"=>{"Unspecified"=>6}, "Environmental Science"=>{"Environmental Science"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>4}}, "reader_count_by_country"=>{"China"=>1}, "group_count"=>7}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84865506513"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84865506513?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84865506513&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84865506513&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84865506513", "dc:identifier"=>"SCOPUS_ID:84865506513", "eid"=>"2-s2.0-84865506513", "dc:title"=>"Identification of Thalidomide-Specific Transcriptomics and Proteomics Signatures during Differentiation of Human Embryonic Stem Cells", "dc:creator"=>"Meganathan K.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"7", "prism:issueIdentifier"=>"8", "prism:pageRange"=>nil, "prism:coverDate"=>"2012-08-28", "prism:coverDisplayDate"=>"28 August 2012", "prism:doi"=>"10.1371/journal.pone.0044228", "citedby-count"=>"63", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"University of Cologne", "affiliation-city"=>"Koln", "affiliation-country"=>"Germany"}], "pubmed-id"=>"22952932", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e44228", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0044228", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Twitter

Counter

  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"8", "xml_views"=>"4", "html_views"=>"103"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"70", "xml_views"=>"1", "html_views"=>"350"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"117"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"43", "xml_views"=>"1", "html_views"=>"127"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"136"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"140"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"110"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"152"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"31", "xml_views"=>"1", "html_views"=>"164"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"135"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"84"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"74"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"84"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"93"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"19", "xml_views"=>"3", "html_views"=>"172"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"218"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"179"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"262"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"130"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"2", "html_views"=>"140"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"146"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"182"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"133"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"113"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"231"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"103"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"120"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"78"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"4", "html_views"=>"57"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"36"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"69"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"43"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"84"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"107"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"89"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"86"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"79"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"2", "html_views"=>"55"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"3", "html_views"=>"46"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"2", "html_views"=>"53"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"34"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"43"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"66"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"8"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"3", "html_views"=>"14"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"22"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"3"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/586644"], "description"=>"<p>The common differentially regulated gene (mRNA (n = 3), (p≤0.05) and protein (n = 3), (p≤0.01)) expression pattern after 14 days differentiation.</p>", "links"=>[], "tags"=>["differentially", "regulated", "14", "days"], "article_id"=>257128, "categories"=>["Molecular Biology", "Biochemistry", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Kesavan Meganathan", "Smita Jagtap", "Vilas Wagh", "Johannes Winkler", "John Antonydas Gaspar", "Diana Hildebrand", "Maria Trusch", "Karola Lehmann", "Jürgen Hescheler", "Hartmut Schlüter", "Agapios Sachinidis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044228.t001", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_common_differentially_regulated_gene_mRNA_n_8202_8202_3_p_8804_0_05_and_protein_n_8202_8202_3_p_8804_0_01_expression_pattern_after_14_days_differentiation_/257128", "title"=>"The common differentially regulated gene (mRNA (n = 3), (p≤0.05) and protein (n = 3), (p≤0.01)) expression pattern after 14 days differentiation.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-08-28 01:58:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/585949"], "description"=>"<p><b>A</b>, A schematic representation of the 14 days hESC differentiation. <b>B</b>, An unsupervised hierarchical clustering analysis of DEG in hESC differentiation showed two distinct clusters for up and down-regulated genes. <b>C</b>, The over expressed neuron development BP (from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044228#pone.0044228.s008\" target=\"_blank\">Table S4</a>) encompasses 86 genes and their gene expression pattern showed in volcano plot. In x-axis represents fold change and y-axis showed FDR-controlled p-values (p≤0.05). <b>D</b>, The pluripotency markers were significantly downregulated (as shown by microarray) after 14 days of differentiation. <b>E</b>, The mass spectrometry analysis of regulated proteins unravelled the up-regulated spots for neuronal development related proteins and (<b>F</b>) the down-regulated spots for pluripotency regulators. The differentially regulated proteins (n = 3), (p≤0.01) are shown with their corresponding spot ID and UniProt ID (E (I), F (I)). Their expression patterns are represented as a percentage of volume (E (II), F (II)). The error bar represents SEM from 3 independent biological replicates.</p>", "links"=>[], "tags"=>["embryonic", "genes", "14", "days", "hesc"], "article_id"=>256429, "categories"=>["Molecular Biology", "Biochemistry", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Kesavan Meganathan", "Smita Jagtap", "Vilas Wagh", "Johannes Winkler", "John Antonydas Gaspar", "Diana Hildebrand", "Maria Trusch", "Karola Lehmann", "Jürgen Hescheler", "Hartmut Schlüter", "Agapios Sachinidis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044228.g002", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Enrichment_of_embryonic_development_associated_genes_in_14_days_of_hESC_differentiation_/256429", "title"=>"Enrichment of embryonic development associated genes in 14 days of hESC differentiation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-08-28 01:47:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/585778"], "description"=>"<p><b>A</b>, Experimental representation of the time kinetic multilineage differentiation. <b>B</b>, An unsupervised hierarchical clustering analysis demonstrates that a temporal gene expression pattern changes during differentiation and the major changes were observed between days 12 to 14. Three independent biological experiments were performed for statistical analysis (p≤0.05). <b>C</b>, The dendrogams pattern of array cluster showed that a two major negative correlation for day 0 to 9 and 12 to 21 differentiation. <b>D</b>, The selected germ layer lineage specific BP (Enrichment p≤0.01) for up-regulated transcripts demonstrated that the numbers of genes are optimal between days 12 and 15. The figure represents (i) endoderm-specific, ii) ectoderm-specific and iii) mesoderm-specific BP. The x-axis represents the days of differentiation, and the y-axis represents number of transcripts.</p>", "links"=>[], "tags"=>["temporal", "profiling", "hesc"], "article_id"=>256269, "categories"=>["Molecular Biology", "Biochemistry", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Kesavan Meganathan", "Smita Jagtap", "Vilas Wagh", "Johannes Winkler", "John Antonydas Gaspar", "Diana Hildebrand", "Maria Trusch", "Karola Lehmann", "Jürgen Hescheler", "Hartmut Schlüter", "Agapios Sachinidis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044228.g001", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_A_temporal_gene_expression_profiling_of_hESC_differentiation_/256269", "title"=>"A temporal gene expression profiling of hESC differentiation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-08-28 01:44:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/586297"], "description"=>"<p><b>A</b>, The heat map shows the microarray expression pattern for the selected genes for heart, limb development (from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044228#pone.0044228.s010\" target=\"_blank\">Table S6D, S6F</a>) and WNT signalling (from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044228#pone.0044228.s013\" target=\"_blank\">Table S9</a>). Since multiple genes are involved in various developmental processes few redundant genes are present. The up-regulated genes in control were repressed in a dose dependent manner. <b>B</b>, The representative heart (I), limb development (II) associated genes were analysed using RT-qPCR analysis with an independent experiment. Thalidomide responsive genes were shown in I and II (*p-value≤0.01, thalidomide-treated vs untreated 14-days old EBs) and 14 days old EBs gene expression were shown in (III) *p-value≤0.01, 14 days old EBs vs ESCs . The error bars represents the SEM from 3 technical replicates. <b>C</b>, The mass spectrometry results showed down-regulation of DDAH2 gene (n = 3), (p≤0.01). Figure C (I) represents spot analysis of DDAH2, mRNA and protein fold change (FC) values. C (II) The immunoblotting analysis of representative genes for thalidomide treatment. <b>D</b>, The immunoflourescence results shows the localisation of β-catenin and the gradient suppression at 70 µM thalidomide treatment. The scale bar represents 20 µm. <b>E</b>, The figure representing limb bud patterning was adapted from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044228#pone.0044228-Niswander1\" target=\"_blank\">[40]</a> and modified. The figure shows the molecular markers that SHH and ZPA (essential for limb development) were affected by thalidomide treatments. The green colour coded genes are more than −1.8 fold down-regulated.</p>", "links"=>[], "tags"=>["perturbed", "wnt", "signalling"], "article_id"=>256787, "categories"=>["Molecular Biology", "Biochemistry", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Kesavan Meganathan", "Smita Jagtap", "Vilas Wagh", "Johannes Winkler", "John Antonydas Gaspar", "Diana Hildebrand", "Maria Trusch", "Karola Lehmann", "Jürgen Hescheler", "Hartmut Schlüter", "Agapios Sachinidis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044228.g004", "stats"=>{"downloads"=>3, "page_views"=>23, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Thalidomide_perturbed_heart_limb_development_and_WNT_signalling_associated_genes_/256787", "title"=>"Thalidomide perturbed heart, limb development and WNT signalling associated genes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-08-28 01:53:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/586501"], "description"=>"<p>To validate microarray expression. <b>A</b>, (I) (RT-qPCR analysis) 14 day's hESC differentiation showed higher GST expression (*p-value≤0.01, 14 days old EBs vs ESCs) and (II) thalidomide treatment (**p-value≤0.05, thalidomide-treated vs untreated 14-days old EBs) repressed GST genes more than −2 fold change. The error bars represent the SEM from 3 technical replicates from an independent experiment. <b>B</b>, Immunoblotting analysis of GSTA1/2 confirms thalidomide induced perturbation at protein concentration. <b>C</b>, The cellular localisation of GSTA1/2 showed pronounced concentration of GSTA on nuclear membrane in control is attenuated for 10 and 70 µM. The scale bar represents 20 µm. <b>D</b>, The genomic and mass spectrometry analysis showed down-regulation of RANBP1. <b>E</b>, Investigation of transporters genes for thalidomide treatment with RT-qPCR analysis (from independent experiment). The error bars represent the SEM from 3 technical replicates. <b>F</b>, Thalidomide mediated protein concentration changes were shown with immunoblotting analysis. <b>G</b>, The sub cellular localisation of RANBP1 is demonstrated with immunoflourescence analysis. The cytoplasmic enrichment of RANBP1 gradient is repressed with thalidomide treatment. The scale bar represents 20 µm.</p>", "links"=>[], "tags"=>["suppressed", "gst", "genes", "nucleocytoplasmic", "transporters", "mrna"], "article_id"=>256980, "categories"=>["Molecular Biology", "Biochemistry", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Kesavan Meganathan", "Smita Jagtap", "Vilas Wagh", "Johannes Winkler", "John Antonydas Gaspar", "Diana Hildebrand", "Maria Trusch", "Karola Lehmann", "Jürgen Hescheler", "Hartmut Schlüter", "Agapios Sachinidis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044228.g005", "stats"=>{"downloads"=>2, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Thalidomide_suppressed_the_GST_genes_and_nucleocytoplasmic_transporters_at_mRNA_and_protein_level_/256980", "title"=>"Thalidomide suppressed the GST genes and nucleocytoplasmic transporters at mRNA and protein level.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-08-28 01:56:20"}
  • {"files"=>["https://ndownloader.figshare.com/files/586691"], "description"=>"<p>GO categories were identified with DAVID analysis using DEG from the respective treatment concentration.</p>", "links"=>[], "tags"=>["down-regulated", "categories", "thalidomide"], "article_id"=>257175, "categories"=>["Molecular Biology", "Biochemistry", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Kesavan Meganathan", "Smita Jagtap", "Vilas Wagh", "Johannes Winkler", "John Antonydas Gaspar", "Diana Hildebrand", "Maria Trusch", "Karola Lehmann", "Jürgen Hescheler", "Hartmut Schlüter", "Agapios Sachinidis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044228.t002", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Selected_down_regulated_significant_p_8804_0_01_GO_categories_after_thalidomide_treatment_/257175", "title"=>"Selected down-regulated significant (p≤0.01) GO categories after thalidomide treatment.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-08-28 01:59:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/586141"], "description"=>"<p><b>A</b>, The experimental approach of hESC differentiation and thalidomide treatment. <b>B</b>, Selected thalidomide concentrations do not induce any cytotoxicity in hESC differentiation. The scale bar represents 250 µm. <b>C</b>, 3D PCA showed that thalidomide treatment induces the changes in the gene expression pattern with increasing concentration. The 3 biological replicates for each sample group are showed in single colour. <b>D</b>, The number of DEG progressively increased with thalidomide concentration. Black shows up-regulated and grey shows down-regulated transcripts. Fold change ≥2 or ≤−2, (p≤0.05). <b>E</b>, An unsupervised hierarchical clustering of DEG demonstrated a dose-dependent repression. The highly expressed genes in untreated control were repressed for thalidomide in a concentration dependent manner. Data represents from 3 biological replicates. <b>F</b>, To quantify the BP the average relative fold change values were calculated manually (for all transcripts present in each GO) and represented for 14 day old EBs and thalidomide treatment. The error bar represents SEM from fold change values for transcripts belong to each GO.</p>", "links"=>[], "tags"=>["thalidomide", "hesc"], "article_id"=>256628, "categories"=>["Molecular Biology", "Biochemistry", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Kesavan Meganathan", "Smita Jagtap", "Vilas Wagh", "Johannes Winkler", "John Antonydas Gaspar", "Diana Hildebrand", "Maria Trusch", "Karola Lehmann", "Jürgen Hescheler", "Hartmut Schlüter", "Agapios Sachinidis"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044228.g003", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Dose_dependent_gene_expression_response_of_thalidomide_in_hESC_differentiation_/256628", "title"=>"Dose-dependent gene expression response of thalidomide in hESC differentiation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-08-28 01:50:28"}
  • {"files"=>["https://ndownloader.figshare.com/files/308506", "https://ndownloader.figshare.com/files/308542", "https://ndownloader.figshare.com/files/308595", "https://ndownloader.figshare.com/files/308652", "https://ndownloader.figshare.com/files/308709", "https://ndownloader.figshare.com/files/308754", "https://ndownloader.figshare.com/files/308822", "https://ndownloader.figshare.com/files/309035", "https://ndownloader.figshare.com/files/309079", "https://ndownloader.figshare.com/files/309109", "https://ndownloader.figshare.com/files/309143", "https://ndownloader.figshare.com/files/309234", "https://ndownloader.figshare.com/files/309265"], "description"=>"<div><p>Embryonic development can be partially recapitulated <em>in vitro</em> by differentiating human embryonic stem cells (hESCs). Thalidomide is a developmental toxicant <em>in vivo</em> and acts in a species-dependent manner. Besides its therapeutic value, thalidomide also serves as a prototypical model to study teratogenecity. Although many <em>in vivo</em> and <em>in vitro</em> platforms have demonstrated its toxicity, only a few test systems accurately reflect human physiology. We used global gene expression and proteomics profiling (two dimensional electrophoresis (2DE) coupled with Tandem Mass spectrometry) to demonstrate hESC differentiation and thalidomide embryotoxicity/teratogenecity with clinically relevant dose(s). Proteome analysis showed loss of POU5F1 regulatory proteins PKM2 and RBM14 and an over expression of proteins involved in neuronal development (such as PAK2, PAFAH1B2 and PAFAH1B3) after 14 days of differentiation. The genomic and proteomic expression pattern demonstrated differential expression of limb, heart and embryonic development related transcription factors and biological processes. Moreover, this study uncovered novel possible mechanisms, such as the inhibition of RANBP1, that participate in the nucleocytoplasmic trafficking of proteins and inhibition of glutathione transferases (GSTA1, GSTA2), that protect the cell from secondary oxidative stress. As a proof of principle, we demonstrated that a combination of transcriptomics and proteomics, along with consistent differentiation of hESCs, enabled the detection of canonical and novel teratogenic intracellular mechanisms of thalidomide.</p> </div>", "links"=>[], "tags"=>["thalidomide-specific", "transcriptomics", "proteomics", "signatures", "differentiation", "embryonic", "cells"], "article_id"=>120863, "categories"=>["Molecular Biology", "Biochemistry", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Kesavan Meganathan", "Smita Jagtap", "Vilas Wagh", "Johannes Winkler", "John Antonydas Gaspar", "Diana Hildebrand", "Maria Trusch", "Karola Lehmann", "Jürgen Hescheler", "Hartmut Schlüter", "Agapios Sachinidis"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0044228.s001", "https://dx.doi.org/10.1371/journal.pone.0044228.s002", "https://dx.doi.org/10.1371/journal.pone.0044228.s003", "https://dx.doi.org/10.1371/journal.pone.0044228.s004", "https://dx.doi.org/10.1371/journal.pone.0044228.s005", "https://dx.doi.org/10.1371/journal.pone.0044228.s006", "https://dx.doi.org/10.1371/journal.pone.0044228.s007", "https://dx.doi.org/10.1371/journal.pone.0044228.s008", "https://dx.doi.org/10.1371/journal.pone.0044228.s009", "https://dx.doi.org/10.1371/journal.pone.0044228.s010", "https://dx.doi.org/10.1371/journal.pone.0044228.s011", "https://dx.doi.org/10.1371/journal.pone.0044228.s012", "https://dx.doi.org/10.1371/journal.pone.0044228.s013"], "stats"=>{"downloads"=>21, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Identification_of_Thalidomide_Specific_Transcriptomics_and_Proteomics_Signatures_during_Differentiation_of_Human_Embryonic_Stem_Cells/120863", "title"=>"Identification of Thalidomide-Specific Transcriptomics and Proteomics Signatures during Differentiation of Human Embryonic Stem Cells", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-08-28 00:14:23"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"75", "full-text"=>"104", "pdf"=>"56", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"25", "supp-data"=>"14", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"71", "full-text"=>"78", "pdf"=>"50", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"26", "full-text"=>"25", "pdf"=>"18", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"31", "full-text"=>"35", "pdf"=>"9", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"3", "cited-by"=>"1", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"29", "full-text"=>"83", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"34", "full-text"=>"38", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"47", "full-text"=>"73", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"50", "full-text"=>"52", "pdf"=>"22", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"28", "full-text"=>"26", "pdf"=>"12", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"24", "full-text"=>"20", "pdf"=>"19", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"17", "full-text"=>"15", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"14", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"6", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"28", "full-text"=>"30", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"19", "full-text"=>"18", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"35", "full-text"=>"33", "pdf"=>"11", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"19", "full-text"=>"18", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"22", "full-text"=>"22", "pdf"=>"11", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"23", "full-text"=>"24", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"18", "full-text"=>"23", "pdf"=>"6", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"22", "full-text"=>"25", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"19", "full-text"=>"22", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"17", "full-text"=>"21", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"21", "full-text"=>"25", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"28", "full-text"=>"31", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"17", "full-text"=>"15", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"20", "full-text"=>"19", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"19", "full-text"=>"15", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"23", "full-text"=>"21", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"6", "cited-by"=>"1", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"20", "full-text"=>"23", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"18", "full-text"=>"11", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"10", "cited-by"=>"1", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"18", "full-text"=>"16", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"24", "full-text"=>"23", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"14", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"21", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"15", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"14", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"18", "full-text"=>"19", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"14", "full-text"=>"17", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"15", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"12", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"20", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"13", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"20", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"1", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"20", "full-text"=>"18", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"11", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"19", "full-text"=>"14", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"18", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}

Relative Metric

{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[322, 550, 671, 773, 864, 955, 1048, 1135, 1223, 1308, 1387, 1465, 1534, 1602, 1673, 1744, 1813, 1885, 1955, 2026, 2093, 2160, 2228, 2290, 2349]}, {"subject_area"=>"/Biology and life sciences/Anatomy and physiology", "average_usage"=>[307, 540, 660, 756, 847, 936, 1025, 1111, 1199, 1282, 1361, 1432]}, {"subject_area"=>"/Biology and life sciences/Developmental biology", "average_usage"=>[313, 562, 690, 796, 893, 986, 1079, 1173, 1257, 1349, 1429, 1506, 1586, 1661, 1730, 1803, 1876, 1945, 2017, 2091, 2163, 2225, 2294, 2362, 2430]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[312, 547, 668, 769, 861, 953, 1046, 1135, 1224, 1307, 1388, 1464, 1536, 1606, 1676, 1744, 1812, 1882, 1954, 2020, 2089, 2155, 2218, 2282, 2344]}]}
Loading … Spinner
There are currently no alerts