Fibrin-Induced Epithelial-to-Mesenchymal Transition of Peritoneal Mesothelial Cells as a Mechanism of Peritoneal Fibrosis: Effects of Pentoxifylline
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{"title"=>"Fibrin-Induced Epithelial-to-Mesenchymal Transition of Peritoneal Mesothelial Cells as a Mechanism of Peritoneal Fibrosis: Effects of Pentoxifylline", "type"=>"journal", "authors"=>[{"first_name"=>"Cheng Chung", "last_name"=>"Fang", "scopus_author_id"=>"7403269887"}, {"first_name"=>"Jenq Wen", "last_name"=>"Huang", "scopus_author_id"=>"7408104710"}, {"first_name"=>"Ren Shi", "last_name"=>"Shyu", "scopus_author_id"=>"7005245188"}, {"first_name"=>"Chung Jen", "last_name"=>"Yen", "scopus_author_id"=>"7202149165"}, {"first_name"=>"Cheng Hsiang", "last_name"=>"Shiao", "scopus_author_id"=>"55361219200"}, {"first_name"=>"Chih Kang", "last_name"=>"Chiang", "scopus_author_id"=>"34967859400"}, {"first_name"=>"Rey Heng", "last_name"=>"Hu", "scopus_author_id"=>"7202640880"}, {"first_name"=>"Tun Jun", "last_name"=>"Tsai", "scopus_author_id"=>"7401925709"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"365666272", "sgr"=>"84866367674", "issn"=>"19326203", "pmid"=>"23028611", "scopus"=>"2-s2.0-84866367674", "doi"=>"10.1371/journal.pone.0044765", "isbn"=>"1932-6203"}, "id"=>"650d2c59-2b42-37c2-8dad-c618e8bfe373", "abstract"=>"Excessive fibrin deposition in the peritoneum is thought to be involved in the development of encapsulating peritoneal sclerosis (EPS), an important cause of morbidity and mortality in peritoneal dialysis patients. We investigated fibrin-induced epithelial-to-mesenchymal transition (EMT) of peritoneal mesothelial cells (PMCs) as a possible mechanism of fibrin involvement in EPS. In vitro, fibrin overlay of PMCs altered their morphology; increased α-smooth muscle actin, fibronectin, fibroblast specific protein-1, and α(v)β(3) integrin expression; and decreased cytokeratin 18 and E-cadherin expression. Fibrin overlay also increased focal adhesion kinase and Src kinase phosphorylation. Fibrin-induced changes were inhibited by treating the cells with α(v)β(3) integrin antibody or pentoxifylline (PTX). In a rat model, intraperitoneal injection of Staphylococcus aureus and fibrinogen induced severe EPS features, which were attenuated by PTX treatment. PTX-treated rats also showed preserved peritoneal ultrafiltration function and lower concentrations of cytokines than the untreated rats. S. aureus- and fibrinogen-injected rats had higher percentage of cytokeratin-positive cells in the omentum fibrotic tissue than controls; this was also reduced by PTX treatment. Our results suggest that fibrin induces EMT of PMCs by engaging α(v)β(3) integrin and activating associated kinases. Our EPS animal model showed that fibrin-induced EMT was involved in the pathogenesis of peritoneal fibrosis and was inhibited by PTX.", "link"=>"http://www.mendeley.com/research/fibrininduced-epithelialtomesenchymal-transition-peritoneal-mesothelial-cells-mechanism-peritoneal-f", "reader_count"=>23, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>5, "Researcher"=>3, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>2, "Student > Master"=>4, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>5, "Researcher"=>3, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>2, "Student > Master"=>4, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>7, "Medicine and Dentistry"=>12, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Chemistry"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>12}, "Chemistry"=>{"Chemistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>7}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"United Kingdom"=>3}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/577609"], "description"=>"<p>Equal amounts of protein (20 µg per lane) from untreated or fibrin-covered PMCs were resolved, transferred, and probed for phosphorylated FAK (focal adhesion kinase) and Src (A). The relative levels of Src/GAPDH (B) and FAK/GAPDH (C) were measured by densitometry. C, PMCs without fibrin; F10, fibrin covered for 10 min; P10, fibrin covered and treated with pentoxifylline 0.3 mg/ml for 10 min; A10, fibrin covered and treated with α<sub>v</sub>β<sub>3</sub> integrin antibody for 10 min; F20, fibrin covered for 20 min; F30, fibrin covered for 30 min. *p<0.05 vs. Control.</p>", "links"=>[], "tags"=>["blots", "integrin-activated"], "article_id"=>248096, "categories"=>["Physiology", "Biochemistry", "Cell Biology", "Marine Biology"], "users"=>["Cheng-Chung Fang", "Jenq-Wen Huang", "Ren-Shi Shyu", "Chung-Jen Yen", "Cheng-Hsiang Shiao", "Chih-Kang Chiang", "Rey-Heng Hu", "Tun-Jun Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044765.g004", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Western_blots_of_integrin_activated_kinases_/248096", "title"=>"Western blots of integrin-activated kinases.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-09-13 02:14:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/578291"], "description"=>"<p>Gross adhesion scoring system.</p>", "links"=>[], "tags"=>["adhesion", "scoring"], "article_id"=>248781, "categories"=>["Physiology", "Biochemistry", "Cell Biology", "Marine Biology"], "users"=>["Cheng-Chung Fang", "Jenq-Wen Huang", "Ren-Shi Shyu", "Chung-Jen Yen", "Cheng-Hsiang Shiao", "Chih-Kang Chiang", "Rey-Heng Hu", "Tun-Jun Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044765.t003", "stats"=>{"downloads"=>3, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Gross_adhesion_scoring_system_/248781", "title"=>"Gross adhesion scoring system.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-09-13 02:26:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/304570", "https://ndownloader.figshare.com/files/304641", "https://ndownloader.figshare.com/files/304699", "https://ndownloader.figshare.com/files/304805"], "description"=>"<div><p>Excessive fibrin deposition in the peritoneum is thought to be involved in the development of encapsulating peritoneal sclerosis (EPS), an important cause of morbidity and mortality in peritoneal dialysis patients. We investigated fibrin-induced epithelial-to-mesenchymal transition (EMT) of peritoneal mesothelial cells (PMCs) as a possible mechanism of fibrin involvement in EPS. <em>In vitro</em>, fibrin overlay of PMCs altered their morphology; increased α-smooth muscle actin, fibronectin, fibroblast specific protein-1, and α<sub>v</sub>β<sub>3</sub> integrin expression; and decreased cytokeratin 18 and E-cadherin expression. Fibrin overlay also increased focal adhesion kinase and Src kinase phosphorylation. Fibrin-induced changes were inhibited by treating the cells with α<sub>v</sub>β<sub>3</sub> integrin antibody or pentoxifylline (PTX). In a rat model, intraperitoneal injection of <em>Staphylococcus aureus</em> and fibrinogen induced severe EPS features, which were attenuated by PTX treatment. PTX-treated rats also showed preserved peritoneal ultrafiltration function and lower concentrations of cytokines than the untreated rats. <em>S. aureus</em>- and fibrinogen-injected rats had higher percentage of cytokeratin-positive cells in the omentum fibrotic tissue than controls; this was also reduced by PTX treatment. Our results suggest that fibrin induces EMT of PMCs by engaging α<sub>v</sub>β<sub>3</sub> integrin and activating associated kinases. Our EPS animal model showed that fibrin-induced EMT was involved in the pathogenesis of peritoneal fibrosis and was inhibited by PTX.</p> </div>", "links"=>[], "tags"=>["fibrin-induced", "epithelial-to-mesenchymal", "peritoneal", "mesothelial", "cells", "effects", "pentoxifylline"], "article_id"=>120073, "categories"=>["Physiology", "Biochemistry", "Cell Biology", "Marine Biology"], "users"=>["Cheng-Chung Fang", "Jenq-Wen Huang", "Ren-Shi Shyu", "Chung-Jen Yen", "Cheng-Hsiang Shiao", "Chih-Kang Chiang", "Rey-Heng Hu", "Tun-Jun Tsai"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0044765.s001", "https://dx.doi.org/10.1371/journal.pone.0044765.s002", "https://dx.doi.org/10.1371/journal.pone.0044765.s003", "https://dx.doi.org/10.1371/journal.pone.0044765.s004"], "stats"=>{"downloads"=>13, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Fibrin_Induced_Epithelial_to_Mesenchymal_Transition_of_Peritoneal_Mesothelial_Cells_as_a_Mechanism_of_Peritoneal_Fibrosis_Effects_of_Pentoxifylline/120073", "title"=>"Fibrin-Induced Epithelial-to-Mesenchymal Transition of Peritoneal Mesothelial Cells as a Mechanism of Peritoneal Fibrosis: Effects of Pentoxifylline", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-09-13 00:01:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/577804"], "description"=>"<p>The compact zones were thicker in Group 4 than in Group 1 on both the parietal peritoneum (A) and liver surface (B). Rats treated with PTX (Group 5) had significantly less submesothelial matrix formation than Group 4 on both the parietal peritoneum and liver surface. *p<0.05 vs. Group 1 (Control), #p<0.05 vs. Group 4.</p>", "links"=>[], "tags"=>["submesothelial", "compact"], "article_id"=>248296, "categories"=>["Physiology", "Biochemistry", "Cell Biology", "Marine Biology"], "users"=>["Cheng-Chung Fang", "Jenq-Wen Huang", "Ren-Shi Shyu", "Chung-Jen Yen", "Cheng-Hsiang Shiao", "Chih-Kang Chiang", "Rey-Heng Hu", "Tun-Jun Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044765.g006", "stats"=>{"downloads"=>2, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Histological_analysis_of_the_submesothelial_compact_zones_/248296", "title"=>"Histological analysis of the submesothelial compact zones.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-09-13 02:18:16"}
  • {"files"=>["https://ndownloader.figshare.com/files/577278"], "description"=>"<p>The morphology of the PMCs changed from a polygonal cobblestone-like appearance (A) to a spindle-shaped form (B) after fibrin overlay for 24 h. (C) Morphological changes were attenuated by treatment with 0.3 mg/ml of pentoxifylline.</p>", "links"=>[], "tags"=>["changes", "peritoneal", "mesothelial", "cells", "fibrin"], "article_id"=>247765, "categories"=>["Physiology", "Biochemistry", "Cell Biology", "Marine Biology"], "users"=>["Cheng-Chung Fang", "Jenq-Wen Huang", "Ren-Shi Shyu", "Chung-Jen Yen", "Cheng-Hsiang Shiao", "Chih-Kang Chiang", "Rey-Heng Hu", "Tun-Jun Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044765.g001", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Morphological_changes_in_peritoneal_mesothelial_cells_PMCs_after_fibrin_application_/247765", "title"=>"Morphological changes in peritoneal mesothelial cells (PMCs) after fibrin application.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-09-13 02:09:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/578250"], "description"=>"<p>Data are expressed as mean ± SEM. PBS, phosphate buffered saline; B, bacteria; F, fibrinogen; PTX, pentoxifylline.</p>*<p>P<0.05 vs. Group 1,</p>†<p>P<0.05 vs. Group 4.</p>", "links"=>[], "tags"=>["concentrations", "peritoneal"], "article_id"=>248739, "categories"=>["Physiology", "Biochemistry", "Cell Biology", "Marine Biology"], "users"=>["Cheng-Chung Fang", "Jenq-Wen Huang", "Ren-Shi Shyu", "Chung-Jen Yen", "Cheng-Hsiang Shiao", "Chih-Kang Chiang", "Rey-Heng Hu", "Tun-Jun Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044765.t002", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cytokine_concentrations_in_peritoneal_dialysates_/248739", "title"=>"Cytokine concentrations in peritoneal dialysates.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-09-13 02:25:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/577879"], "description"=>"<p>TGFβ1 mRNA was measured by RT-PCR. mRNA levels increased in Groups 2 and 4 compared to Group 1 but expression in Groups 2 and 5 was significantly lower than in Group 4. GAPDH expression served as a loading control. *p<0.05 vs. Group 1, #p<0.05 vs. Group 4.</p>", "links"=>[], "tags"=>["parietal"], "article_id"=>248372, "categories"=>["Physiology", "Biochemistry", "Cell Biology", "Marine Biology"], "users"=>["Cheng-Chung Fang", "Jenq-Wen Huang", "Ren-Shi Shyu", "Chung-Jen Yen", "Cheng-Hsiang Shiao", "Chih-Kang Chiang", "Rey-Heng Hu", "Tun-Jun Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044765.g007", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_TGF_946_1_gene_expression_in_the_parietal_peritoneum_/248372", "title"=>"TGFβ1 gene expression in the parietal peritoneum.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-09-13 02:19:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/577502"], "description"=>"<p>Equal amounts of protein (20 µg per lane) from untreated or fibrin-covered PMCs were resolved, transferred, and blotted for α-SMA, fibronectin, FSP-1, α<sub>v</sub>β<sub>3</sub> integrin, cytokeratin 18, E-cadherin, and GAPDH (A). The relative levels of α-SMA/GAPDH (B), α<sub>v</sub>β<sub>3</sub> integrin/GAPDH (C and D), fibronectin/GAPDH (E), FSP-1/GAPDH (F), cytokeratin 18/GAPDH (G), and E-cadherin/GAPDH (H) were measured by densitometry. C, PMCs without fibrin; F1, fibrin covered for 1 h; P1, fibrin covered and treated with pentoxifylline (PTX) 0.3 mg/ml for 1 h; F4, fibrin covered for 4 h, P4, fibrin covered and treated with PTX 0.3 mg/ml for 4 h; A4, fibrin covered and treated with α<sub>v</sub>β<sub>3</sub> integrin antibody for 4 h. *P<0.05 vs. C, # P<0.05 between groups, n = 3.</p>", "links"=>[], "tags"=>["blots", "markers", "fibrin-covered", "peritoneal", "mesothelial", "cells"], "article_id"=>247982, "categories"=>["Physiology", "Biochemistry", "Cell Biology", "Marine Biology"], "users"=>["Cheng-Chung Fang", "Jenq-Wen Huang", "Ren-Shi Shyu", "Chung-Jen Yen", "Cheng-Hsiang Shiao", "Chih-Kang Chiang", "Rey-Heng Hu", "Tun-Jun Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044765.g003", "stats"=>{"downloads"=>1, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Western_blots_of_cell_markers_in_fibrin_covered_peritoneal_mesothelial_cells_PMCs_/247982", "title"=>"Western blots of cell markers in fibrin-covered peritoneal mesothelial cells (PMCs).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-09-13 02:13:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/577714"], "description"=>"<p>Group 4 had a significantly higher score than Group 1. Group 5 had a significantly lower score than Group 4. *p<0.05 vs. Group 1, #p<0.05 vs. Group 4.</p>", "links"=>[], "tags"=>["adhesion"], "article_id"=>248207, "categories"=>["Physiology", "Biochemistry", "Cell Biology", "Marine Biology"], "users"=>["Cheng-Chung Fang", "Jenq-Wen Huang", "Ren-Shi Shyu", "Chung-Jen Yen", "Cheng-Hsiang Shiao", "Chih-Kang Chiang", "Rey-Heng Hu", "Tun-Jun Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044765.g005", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Gross_adhesion_scores_/248207", "title"=>"Gross adhesion scores.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-09-13 02:16:47"}
  • {"files"=>["https://ndownloader.figshare.com/files/578102"], "description"=>"<p>Equal amounts of protein (30 µg per lane) from the peritoneum of rats in Group 1 to 5 were resolved, transferred, and probed for α-SMA, α<sub>v</sub>β<sub>3</sub> integrin, fibronectin, FSP-1, and GAPDH (A). The relative levels of α-SMA/GAPDH (B), α<sub>v</sub>β<sub>3</sub> integrin/GAPDH (C and D), fibronectin/GAPDH (E), and FSP-1/GAPDH (F) were measured by densitometry.*p<0.05 vs. Group 1, #p<0.05 vs. Group 4.</p>", "links"=>[], "tags"=>["blots", "markers", "peritoneum", "animals"], "article_id"=>248590, "categories"=>["Physiology", "Biochemistry", "Cell Biology", "Marine Biology"], "users"=>["Cheng-Chung Fang", "Jenq-Wen Huang", "Ren-Shi Shyu", "Chung-Jen Yen", "Cheng-Hsiang Shiao", "Chih-Kang Chiang", "Rey-Heng Hu", "Tun-Jun Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044765.g009", "stats"=>{"downloads"=>4, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Western_blots_of_cell_markers_in_the_peritoneum_of_animals_with_EPS_/248590", "title"=>"Western blots of cell markers in the peritoneum of animals with EPS.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-09-13 02:23:10"}
  • {"files"=>["https://ndownloader.figshare.com/files/578163"], "description"=>"<p>Thirty male Wistar rats were randomly divided into 5 groups of 6 rats per group. Rats received intraperitoneal injections of PBS (Group 1), <i>S. aureus</i> (6×10<sup>8</sup> colony forming units (CFU); Group 2), 15 ml fibrinogen (10 mg/ml; Group 3), or both <i>S. aureus</i> and fibrinogen (Group 4). Group 5 animals received intraperitoneal injections of <i>S. aureus</i> and fibrinogen and also received daily intravenous injections of PTX (100 mg/kg) for 7 days. Animals were then subjected to the peritoneal equilibration test (PET) and sacrificed. The details of procedures are shown in the figure. ▾: Catheter placement in the internal jugular vein. ○: PBS intraperitoneally. •: 1 ml <i>S. aureu</i>s (6×10<sup>8</sup> CFU/ml) intraperitoneally. ◊: 15 ml PBS intraperitoneally. ⧫: 15 ml fibrinogen (10 mg/ml) intraperitoneally. ▪ ▪ ▪ ▪ ▪: 1.5 ml intravenous PBS daily for 7 days. ▪▪▪▪▪▪: 100 mg/kg intravenous pentoxifylline (20 mg/ml; approximately 1.5 ml) daily for 7 days. ×: Performed PET and then sacrificed.</p>", "links"=>[], "tags"=>["Nephrology", "physiology", "cell biology", "Biochemistry"], "article_id"=>248651, "categories"=>["Physiology", "Biochemistry", "Cell Biology", "Marine Biology"], "users"=>["Cheng-Chung Fang", "Jenq-Wen Huang", "Ren-Shi Shyu", "Chung-Jen Yen", "Cheng-Hsiang Shiao", "Chih-Kang Chiang", "Rey-Heng Hu", "Tun-Jun Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044765.g010", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Protocol_for_the_animal_study_/248651", "title"=>"Protocol for the animal study.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-09-13 02:24:11"}
  • {"files"=>["https://ndownloader.figshare.com/files/577375"], "description"=>"<p>Expression of α-smooth muscle actin (α-SMA), fibronectin, fibroblast specific protein-1 (FSP-1), and β<sub>3</sub> integrin were detected by immunofluorescence staining with FITC-labeled secondary antibodies (green). Nuclei were counterstained with PI (red). PMCs overlaid with fibrin for 4 h (+Fibrin) expressed higher levels of α-SMA, fibronectin, FSP-1, and β<sub>3</sub> integrin than untreated PMCs (-Fibrin). Original magnification ×400.</p>", "links"=>[], "tags"=>["markers", "fibrin", "peritoneal", "mesothelial", "cells"], "article_id"=>247859, "categories"=>["Physiology", "Biochemistry", "Cell Biology", "Marine Biology"], "users"=>["Cheng-Chung Fang", "Jenq-Wen Huang", "Ren-Shi Shyu", "Chung-Jen Yen", "Cheng-Hsiang Shiao", "Chih-Kang Chiang", "Rey-Heng Hu", "Tun-Jun Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044765.g002", "stats"=>{"downloads"=>1, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Changes_in_cell_markers_after_application_of_fibrin_to_peritoneal_mesothelial_cells_PMCs_/247859", "title"=>"Changes in cell markers after application of fibrin to peritoneal mesothelial cells (PMCs).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-09-13 02:10:59"}
  • {"files"=>["https://ndownloader.figshare.com/files/577988"], "description"=>"<p>(A) Cytokeratin-positive cells were present not only on the surface mesothelium, but also in the submesothelial fibrosis on the liver surface, parietal peritoneum, and omentum of Group 4 rats. Cytokeratin-positive cells were present only on the surface mesothelium of Group 1 rats. Original magnification ×400. (B) Percentage of cytokeratin-positive cells in the fibrotic tissue of the omentum. *p<0.05 vs. Group 1, #p<0.05.</p>", "links"=>[], "tags"=>["staining"], "article_id"=>248481, "categories"=>["Physiology", "Biochemistry", "Cell Biology", "Marine Biology"], "users"=>["Cheng-Chung Fang", "Jenq-Wen Huang", "Ren-Shi Shyu", "Chung-Jen Yen", "Cheng-Hsiang Shiao", "Chih-Kang Chiang", "Rey-Heng Hu", "Tun-Jun Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044765.g008", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Immunohistochemical_staining_of_cytokeratin_/248481", "title"=>"Immunohistochemical staining of cytokeratin.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-09-13 02:21:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/578209"], "description"=>"<p>Data are expressed as mean ± SEM. D2, dialysate after 2 h dwell; D0, dialysate at the beginning of peritoneal equilibrium test; S, serum; PBS, phosphate buffered saline; B, bacteria; F, fibrinogen; PTX, pentoxifylline.</p>*<p>P<0.05 vs. Group 1,</p>†<p>P<0.05 vs. Group 4.</p>", "links"=>[], "tags"=>["equilibrium"], "article_id"=>248703, "categories"=>["Physiology", "Biochemistry", "Cell Biology", "Marine Biology"], "users"=>["Cheng-Chung Fang", "Jenq-Wen Huang", "Ren-Shi Shyu", "Chung-Jen Yen", "Cheng-Hsiang Shiao", "Chih-Kang Chiang", "Rey-Heng Hu", "Tun-Jun Tsai"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044765.t001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Peritoneal_equilibrium_tests_/248703", "title"=>"Peritoneal equilibrium tests.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-09-13 02:25:03"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"48", "full-text"=>"53", "pdf"=>"32", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
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  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"38", "full-text"=>"42", "pdf"=>"27", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"21", "full-text"=>"22", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"19", "full-text"=>"13", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"15", "full-text"=>"12", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"24", "full-text"=>"27", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"17", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
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  • {"unique-ip"=>"14", "full-text"=>"9", "pdf"=>"7", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"5", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
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