Complementation between Two Tospoviruses Facilitates the Systemic Movement of a Plant Virus Silencing Suppressor in an Otherwise Restrictive Host
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{"title"=>"Complementation between Two Tospoviruses Facilitates the Systemic Movement of a Plant Virus Silencing Suppressor in an Otherwise Restrictive Host", "type"=>"journal", "authors"=>[{"first_name"=>"Sudeep", "last_name"=>"Bag", "scopus_author_id"=>"26428007700"}, {"first_name"=>"Neena", "last_name"=>"Mitter", "scopus_author_id"=>"6602748029"}, {"first_name"=>"Sahar", "last_name"=>"Eid", "scopus_author_id"=>"35217621100"}, {"first_name"=>"Hanu R.", "last_name"=>"Pappu", "scopus_author_id"=>"35563591000"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"23077485", "doi"=>"10.1371/journal.pone.0044803", "pui"=>"365872744", "issn"=>"19326203", "sgr"=>"84867593207", "scopus"=>"2-s2.0-84867593207"}, "id"=>"9911518c-0810-3a6d-a004-3d960efbba9e", "abstract"=>"BACKGROUND: New viruses pathogenic to plants continue to emerge due to mutation, recombination, or reassortment among genomic segments among individual viruses. Tospoviruses cause significant economic damage to a wide range of crops in many parts of the world. The genetic or molecular basis of the continued emergence of new tospoviruses and new hosts is not well understood though it is generally accepted that reassortment and/or genetic complementation among the three genomic segments of individual viruses could be contributing to this variability since plants infected with more than one tospovirus are not uncommon in nature. METHODOLOGY/PRINCIPAL FINDINGS: Two distinct and economically important tospoviruses, Iris yellow spot virus (IYSV) and Tomato spotted wilt virus (TSWV), were investigated for inter-virus interactions at the molecular level in dually-infected plants. Datura (Datura stramonium) is a permissive host for TSWV, while it restricts the movement of IYSV to inoculated leaves. In plants infected with both viruses, however, TSWV facilitated the selective movement of the viral gene silencing suppressor (NSs) gene of IYSV to the younger, uninoculated leaves. The small RNA expression profiles of IYSV and TSWV in single- and dually-infected datura plants showed that systemic leaves of dually-infected plants had reduced levels of TSWV N gene-specific small interfering RNAs (siRNAs). No TSWV NSs-specific siRNAs were detected either in the inoculated or systemic leaves of dually-infected datura plants indicating a more efficient suppression of host silencing machinery in the presence of NSs from both viruses as compared to the presence of only TSWV NSs. CONCLUSION/SIGNIFICANCE: Our study identifies a new role for the viral gene silencing suppressor in potentially modulating the biology and host range of viruses and underscores the importance of virally-coded suppressors of gene silencing in virus infection of plants. This is the first experimental evidence of functional complementation between two distinct tospoviruses in the Bunyaviridae family.", "link"=>"http://www.mendeley.com/research/complementation-between-two-tospoviruses-facilitates-systemic-movement-plant-virus-silencing-suppres", "reader_count"=>40, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>3, "Librarian"=>1, "Researcher"=>11, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>11, "Student > Postgraduate"=>2, "Student > Master"=>4, "Other"=>2, "Lecturer > Senior Lecturer"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>3, "Librarian"=>1, "Researcher"=>11, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>11, "Student > Postgraduate"=>2, "Student > Master"=>4, "Other"=>2, "Lecturer > Senior Lecturer"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>4, "Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>3, "Agricultural and Biological Sciences"=>32}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>32}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Unspecified"=>{"Unspecified"=>4}}, "reader_count_by_country"=>{"Venezuela"=>1, "United States"=>1, "Japan"=>1, "Algeria"=>1, "Australia"=>1, "Croatia"=>1}, "group_count"=>2}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/557894"], "description"=>"*<p><i>D. stramonium</i> plants infected with IYSV or TSWV alone, or co-inoculated were tested for the presence of IYSV non structural protein (NSs) using direct antigen coated-enzyme linked immunosorbent assay, and the IYSV and TSWV nucleocapsid (N) proteins using double antibody sandwich enzyme-linked immunosorbent assay. N/A: not applicable as the antisera are specific to homologous antigens and do not cross react. The A405 values of uninfected (healthy) controls ranged from0.12 for 0.14 for all three antisera.</p>", "links"=>[], "tags"=>["spotted", "wilt"], "article_id"=>228394, "categories"=>["Virology", "Molecular Biology", "Microbiology", "Plant Biology"], "users"=>["Sudeep Bag", "Neena Mitter", "Sahar Eid", "Hanu R. Pappu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044803.t001", "stats"=>{"downloads"=>1, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Detection_of_Iris_yellow_spot_virus_IYSV_and_Tomato_spotted_wilt_virus_TSWV_in_Datura_stramonium_plants_/228394", "title"=>"Detection of <i>Iris yellow spot virus</i> (IYSV) and <i>Tomato spotted wilt virus</i> (TSWV) in <i>Datura stramonium</i> plants.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-19 21:39:03"}
  • {"files"=>["https://ndownloader.figshare.com/files/557008"], "description"=>"<p>(A) IYSV infection is localized in inoculated leaves; (B) TSWV causes systemic infection; (C) Co-inoculation of IYSV and TSWV results in severe systemic infection.</p>", "links"=>[], "tags"=>["differential", "spotted", "wilt"], "article_id"=>227498, "categories"=>["Virology", "Molecular Biology", "Microbiology", "Plant Biology"], "users"=>["Sudeep Bag", "Neena Mitter", "Sahar Eid", "Hanu R. Pappu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044803.g001", "stats"=>{"downloads"=>3, "page_views"=>45, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Schematic_representation_of_Datura_stramonium_as_a_differential_host_to_Iris_yellow_spot_virus_IYSV_and_Tomato_spotted_wilt_virus_TSWV_/227498", "title"=>"Schematic representation of <i>Datura stramonium</i> as a differential host to <i>Iris yellow spot virus</i> (IYSV) and <i>Tomato spotted wilt virus</i> (TSWV).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:34:20"}
  • {"files"=>["https://ndownloader.figshare.com/files/557094"], "description"=>"<p>(A) Inoculated leaves with local lesions as a result of virus infection; (B) younger, uninoculated leaves of the same plant remain symptomless and virus-free; (C) Detection of IYSV genes in inoculated leaves of <i>D. stramonium</i> using reverse transcription-polymerase chain reaction. Total RNA from TSWV inoculated leaf (1) and healthy plant leaf as negative control (2) was used as a template for amplifying the genes using specific primers as described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044803#pone-0044803-t001\" target=\"_blank\">Table 1</a>. M: 1 kb Marker; N: IYSV nucleocapsid (N) protein gene; NSs: IYSV nonstructural (NSs) protein gene; NSm: IYSV nonstructural (NSm) protein gene; G<sub>N</sub>/G<sub>C</sub>: IYSV glycoprotein precursor (G<sub>N</sub>/G<sub>C</sub>); RdRp: IYSV RNA dependent RNA polymerase (RdRp).</p>", "links"=>[], "tags"=>["restrictive"], "article_id"=>227591, "categories"=>["Virology", "Molecular Biology", "Microbiology", "Plant Biology"], "users"=>["Sudeep Bag", "Neena Mitter", "Sahar Eid", "Hanu R. Pappu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044803.g002", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Datura_stramonium_is_a_restrictive_host_to_infection_by_Iris_yellow_spot_virus_IYSV_/227591", "title"=>"<i>Datura stramonium</i> is a restrictive host to infection by <i>Iris yellow spot virus</i> (IYSV).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:34:52"}
  • {"files"=>["https://ndownloader.figshare.com/files/557925"], "description"=>"<p>List of primers used in reverse transcription-polymerase chain reaction.</p>", "links"=>[], "tags"=>["primers", "transcription-polymerase"], "article_id"=>228423, "categories"=>["Virology", "Molecular Biology", "Microbiology", "Plant Biology"], "users"=>["Sudeep Bag", "Neena Mitter", "Sahar Eid", "Hanu R. Pappu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044803.t002", "stats"=>{"downloads"=>13, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_List_of_primers_used_in_reverse_transcription_polymerase_chain_reaction_/228423", "title"=>"List of primers used in reverse transcription-polymerase chain reaction.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-19 21:39:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/557817"], "description"=>"<p>The leaf samples were harvested 25–30 days after inoculation from inoculated and systemic leaves A: TSWV N gene specific small interfering (Si) RNAs B: TSWV NSs gene-specific siRNAs, U6: Ubiquitin as loading control for RNA. Lanes: 1-Healthy, uninfected control; 2- IYSV-inoculated leaf; 3- IYSV systemic leaf; 4-Blank; 5-IYSV+ TSWV-inoculated leaf; 6-IYSV+ TSWV systemic leaf; 7-Blank; 8-TSWV-inoculated leaf; 9-TSWV systemic leaf.</p>", "links"=>[], "tags"=>["interfering", "rnas", "nss", "genes", "spotted", "wilt", "dually", "infected"], "article_id"=>228305, "categories"=>["Virology", "Molecular Biology", "Microbiology", "Plant Biology"], "users"=>["Sudeep Bag", "Neena Mitter", "Sahar Eid", "Hanu R. Pappu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044803.g007", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Small_interfering_si_RNAs_of_N_and_NSs_genes_of_Tomato_spotted_wilt_virus_TSWV_in_single_or_dually_infected_Datura_stramonium_plants_/228305", "title"=>"Small interfering (si) RNAs of N and NSs genes of <i>Tomato spotted wilt virus</i> (TSWV) in single or dually infected <i>Datura stramonium</i> plants.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:38:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/557591"], "description"=>"<p>Detection of nucleocapsid (N) gene and non-structural (NSs) genes in inoculated and uninoculated systemic leaves of <i>Datura stramonium</i> plants infected with <i>Iris yellow spot virus</i> (IYSV) and <i>Tomato spotted wilt virus</i> (TSWV) in plants inoculated with either IYSV, TSWV or both.</p>", "links"=>[], "tags"=>["nucleocapsid", "non-structural", "genes", "inoculated", "uninoculated", "systemic", "leaves", "plants", "infected", "spotted", "wilt", "tswv"], "article_id"=>228066, "categories"=>["Virology", "Molecular Biology", "Microbiology", "Plant Biology"], "users"=>["Sudeep Bag", "Neena Mitter", "Sahar Eid", "Hanu R. Pappu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044803.g005", "stats"=>{"downloads"=>1, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Detection_of_nucleocapsid_N_gene_and_non_structural_NSs_genes_in_inoculated_and_uninoculated_systemic_leaves_of_Datura_stramonium_plants_infected_with_Iris_yellow_spot_virus_IYSV_and_Tomato_spotted_wilt_virus_TSWV_in_plants_inoculated_with_either_IYSV_TS/228066", "title"=>"Detection of nucleocapsid (N) gene and non-structural (NSs) genes in inoculated and uninoculated systemic leaves of <i>Datura stramonium</i> plants infected with <i>Iris yellow spot virus</i> (IYSV) and <i>Tomato spotted wilt virus</i> (TSWV) in plants inoculated with either IYSV, TSWV or both.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:37:24"}
  • {"files"=>["https://ndownloader.figshare.com/files/557722"], "description"=>"<p>Leaf samples were harvested 15–20 days post inoculation from inoculated and systemic leaves. Lanes: 1-Healthy, uninfected control; 2-IYSV only inoculated leaf; 3-IYSV only systemic leaf; 4-TSWV only inoculated leaf; 5-TSWV only systemic leaf; 6-IYSV+ TSWV-inoculated leaf; 7-IYSV+ TSWV systemic leaf.</p>", "links"=>[], "tags"=>["nss", "genes", "spotted", "wilt", "dually", "infected", "plants", "gene-specific", "cdna"], "article_id"=>228206, "categories"=>["Virology", "Molecular Biology", "Microbiology", "Plant Biology"], "users"=>["Sudeep Bag", "Neena Mitter", "Sahar Eid", "Hanu R. Pappu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044803.g006", "stats"=>{"downloads"=>2, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_of_N_and_NSs_genes_of_Iris_yellow_spot_virus_IYSV_and_Tomato_spotted_wilt_virus_TSWV_in_single_or_dually_infected_Datura_stramonium_plants_using_gene_specific_cDNA_probes_/228206", "title"=>"Expression of N and NSs genes of <i>Iris yellow spot virus</i> (IYSV) and <i>Tomato spotted wilt virus</i> (TSWV) in single or dually infected <i>Datura stramonium</i> plants using gene-specific cDNA probes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:38:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/557198"], "description"=>"<p><i>D. stramonium</i> infected with TSWV (A) Inoculated leaves with local lesions as a result of virus infection; (B) Younger, uninoculated leaves of the same plant shows severe necrotic symptoms; (C) Detection of TSWV genes in inoculated leaves of <i>D. stramonium</i> using reverse transcription-polymerase chain reaction. Total RNA from TSWV inoculated leaf (1) and healthy plant leaf as negative control (2) was used as a template for amplifying the genes using specific primers as described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044803#pone-0044803-t001\" target=\"_blank\">Table 1</a>. M: 1 kb Marker; N: IYSV nucleocapsid (N) protein gene; NSs: IYSV nonstructural (NSs) protein gene; NSm: IYSV nonstructural (NSm) protein gene; G<sub>N</sub>/G<sub>C</sub>: IYSV glycoprotein precursor (G<sub>N</sub>/G<sub>C</sub>); RdRp: IYSV RNA dependent RNA polymerase (RdRp).</p>", "links"=>[], "tags"=>["permissive", "spotted", "wilt"], "article_id"=>227690, "categories"=>["Virology", "Molecular Biology", "Microbiology", "Plant Biology"], "users"=>["Sudeep Bag", "Neena Mitter", "Sahar Eid", "Hanu R. Pappu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044803.g003", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Datura_stramonium_is_a_permissive_host_for_Tomato_spotted_wilt_virus_TSWV_/227690", "title"=>"<i>Datura stramonium</i> is a permissive host for <i>Tomato spotted wilt virus</i> (TSWV).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:35:24"}
  • {"files"=>["https://ndownloader.figshare.com/files/557378"], "description"=>"<p>(A) Leaves inoculated with both IYSV and TSWV Dual infection results in more severe systemic symptoms, necrotic spots and concentric rings on inoculated leaves; (B) systemic leaves from dual inoculated plants showing symptoms including leaf curling, severe venial chlorosis, and yellowing on younger un-inoculated leaves; (C) Detection of IYSV genes in systemic leaves of <i>D. stramonium</i> co-inoculated with TSWV and IYSV using reverse transcription-polymerase chain reaction., (D) Detection of TSWV genes in systemic leaves of <i>D. stramonium</i> co-inoculated with TSWV and IYSV using reverse transcription-polymerase chain reaction; Lane M: Marker; N: nucleocapsid (N) protein gene; NSs: nonstructural (NSs) protein gene; NSm: nonstructural (NSm) protein gene; G<sub>N</sub>/G<sub>C</sub>: glycoprotein precursor (G<sub>N</sub>/G<sub>C</sub>); RdRp: RNA dependent RNA polymerase (RdRp);.</p>", "links"=>[], "tags"=>["plants", "co-inoculated", "spotted", "wilt"], "article_id"=>227873, "categories"=>["Virology", "Molecular Biology", "Microbiology", "Plant Biology"], "users"=>["Sudeep Bag", "Neena Mitter", "Sahar Eid", "Hanu R. Pappu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044803.g004", "stats"=>{"downloads"=>4, "page_views"=>98, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Datura_stramonium_plants_co_inoculated_with_Iris_yellow_spot_virus_IYSV_and_Tomato_spotted_wilt_virus_TSWV_/227873", "title"=>"<i>Datura stramonium</i> plants co-inoculated with <i>Iris yellow spot virus</i> (IYSV) and <i>Tomato spotted wilt virus</i> (TSWV).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:36:24"}

PMC Usage Stats | Further Information

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Relative Metric

{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[316, 541, 663, 766, 856, 950, 1041, 1128, 1218, 1302, 1382, 1456, 1526, 1593, 1657, 1729, 1796, 1862, 1930, 1999, 2065, 2132, 2202, 2261, 2319]}, {"subject_area"=>"/Biology and life sciences/Plant science", "average_usage"=>[329, 543, 667, 773, 865, 963, 1066, 1149, 1233, 1323, 1411, 1500, 1588, 1661, 1732, 1803, 1887, 1978, 2057, 2138, 2203, 2283, 2363, 2419, 2493]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[312, 547, 668, 769, 861, 953, 1046, 1135, 1224, 1307, 1388, 1464, 1536, 1606, 1676, 1744, 1812, 1882, 1954, 2020, 2089, 2155, 2218, 2282, 2344]}]}
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