Parvin Overexpression Uncovers Tissue-Specific Genetic Pathways and Disrupts F-Actin to Induce Apoptosis in the Developing Epithelia in Drosophila
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{"title"=>"Parvin Overexpression Uncovers Tissue-Specific Genetic Pathways and Disrupts F-Actin to Induce Apoptosis in the Developing Epithelia in Drosophila", "type"=>"journal", "authors"=>[{"first_name"=>"Maria", "last_name"=>"Chountala", "scopus_author_id"=>"55392011200"}, {"first_name"=>"Katerina M.", "last_name"=>"Vakaloglou", "scopus_author_id"=>"45761659700"}, {"first_name"=>"Christos G.", "last_name"=>"Zervas", "scopus_author_id"=>"56308339700"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84867496144", "doi"=>"10.1371/journal.pone.0047355", "pui"=>"365864475", "pmid"=>"23077599", "scopus"=>"2-s2.0-84867496144", "issn"=>"19326203"}, "id"=>"233604e2-25fc-3f49-b82b-41f6f6f46baf", "abstract"=>"Parvin is a putative F-actin binding protein important for integrin-mediated cell adhesion. Here we used overexpression of Drosophila Parvin to uncover its functions in different tissues in vivo. Parvin overexpression caused major defects reminiscent of metastatic cancer cells in developing epithelia, including apoptosis, alterations in cell shape, basal extrusion and invasion. These defects were closely correlated with abnormalities in the organization of F-actin at the basal epithelial surface and of integrin-matrix adhesion sites. In wing epithelium, overexpressed Parvin triggered increased Rho1 protein levels, predominantly at the basal side, whereas in the developing eye it caused a rough eye phenotype and severely disrupted F-actin filaments at the retina floor of pigment cells. We identified genes that suppressed these Parvin-induced dominant effects, depending on the cell type. Co-expression of both ILK and the apoptosis inhibitor DIAP1 blocked Parvin-induced lethality and apoptosis and partially ameliorated cell delamination in epithelia, but did not rescue the elevated Rho1 levels, the abnormal organization of F-actin in the wing and the assembly of integrin-matrix adhesion sites. The rough eye phenotype was suppressed by coexpression of either PTEN or Wech, or by knock-down of Xrp1. Two main conclusions can be drawn from our studies: (1), high levels of cytoplasmic Parvin are toxic in epithelial cells; (2) Parvin in a dose dependent manner affects the organization of actin cytoskeleton in both wing and eye epithelia, independently of its role as a structural component of the ILK-PINCH-Parvin complex that mediates the integrin-actin link. Thus, distinct genetic interactions of Parvin occur in different cell types and second site modifier screens are required to uncover such genetic circuits.", "link"=>"http://www.mendeley.com/research/parvin-overexpression-uncovers-tissuespecific-genetic-pathways-disrupts-factin-induce-apoptosis-deve", "reader_count"=>20, "reader_count_by_academic_status"=>{"Researcher"=>7, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>8, "Student > Master"=>3, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Researcher"=>7, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>8, "Student > Master"=>3, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>6, "Agricultural and Biological Sciences"=>13, "Medicine and Dentistry"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>13}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}}, "reader_count_by_country"=>{"France"=>1}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/559603"], "description"=>"<p>Confocal optical sections acquired from wing imaginal discs of late third instar larvae expressing <i>UAS::Parvin-GFP</i> (green, A–F; white, A′′–B′′) or <i>UAS::Parvin<sup>ΔCH2</sup>-GFP</i> (green, G) under <i>enGal4</i> in the posterior compartment of the disc, probed for laminin (red, A–A1′′; white A′), βPS integrin (red, B; white B′, B1′), Ena (magenta, C–E; white C′–E′), and Cadherin (magenta, F–G; white F′–G′). Panels C–D show two different focal planes: basal (C), and intermediate (D) sides of the epithelium. (A1–A1′′, E–E′) cross optical sections of the imaginal discs appearing in images A and C respectively taken in the middle of the wing poutch. Small arrows, closed areas in the posterior and anterior compartment of the wing pouch expressing (right) or not expressing (left) <i>UAS::Parvin-GFP</i>; big arrows, indicate areas with LamininA deposition; big dashed arrows indicate areas without LamininA deposition; open arrows, apical boundaries of wing epithelial cells; arrowheads, focal contact-like structures in the basal side of the anterior compartment; open arrowheads, areas lacking integrin-matrix adhesion sites. The anterior part of the wing disc (where Parvin-GFP expression is not induced) serves as an internal control.</p>", "links"=>[], "tags"=>["overexpression", "epithelium", "disrupts", "basal", "adhesion", "affecting", "apicobasal"], "article_id"=>230091, "categories"=>["Molecular Biology", "Biological Sciences", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Maria Chountala", "Katerina M. Vakaloglou", "Christos G. Zervas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047355.g005", "stats"=>{"downloads"=>4, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Parvin_overexpression_in_the_wing_epithelium_disrupts_basal_adhesion_without_affecting_apicobasal_polarity_/230091", "title"=>"Parvin overexpression in the wing epithelium disrupts basal adhesion without affecting apicobasal polarity.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:48:06"}
  • {"files"=>["https://ndownloader.figshare.com/files/561580"], "description"=>"<p>Confocal optical sections acquired basally, in developing pupae eyes expressing control <i>UAS::Parvin<sup>ΔCH2</sup>-GFP</i> (A, green; A′′, white), or <i>UAS::Parvin-GFP</i> (B, green; B′′, white) with <i>longGMRGal4</i>, probed with rhodamine-phalloidin to visualize F-actin (red, A–B; white A′–B′) and stained with DAPI to visualize the nuclei (blue, A–B; white A′′′–B′′′). The pattern of F-actin in the retina floor is illustrated with the arrow.</p>", "links"=>[], "tags"=>["overexpression", "pigment", "cells", "disrupts", "f-actin", "cytoskeleton"], "article_id"=>232068, "categories"=>["Molecular Biology", "Biological Sciences", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Maria Chountala", "Katerina M. Vakaloglou", "Christos G. Zervas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047355.g013", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Parvin_overexpression_in_the_eye_pigment_cells_disrupts_F_actin_cytoskeleton_basally_/232068", "title"=>"Parvin overexpression in the eye pigment cells disrupts F-actin cytoskeleton basally.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:58:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/560756"], "description"=>"<p>Confocal optical sections acquired from wing imaginal discs of late third instar larvae expressing <i>UAS::Parvin-GFP</i> (green, A–B, A1–A1′; white, A′′–B′′) with both <i>UAS:ILK</i> and <i>UAS:DIAP1</i> driven by <i>enGal4</i> in the posterior compartment and probed for LamininA (red, A, A1–A1′; white, A′) or βPS integrin (red, B; white, B′) and DAPI to visualize nuclei (blue, A, A1, A1′′). (A1) cross optical section of the imaginal disc appearing in image A taken in the middle of the wing poutch. Small arrows, closed areas in the posterior and anterior compartment of the wing pouch expressing (right) or not expressing (left) <i>UAS::Parvin-GFP</i>; big arrows, indicate areas with LamininA deposition; big dashed arrows indicate areas without LamininA deposition; arrowheads, focal contact-like structures in the basal side of the anterior compartment and open arrowheads indicate areas in the posterior compartment without integrin accumulation. The anterior part of the wing disc (where Parvin-GFP expression is not induced) serves as an internal control.</p>", "links"=>[], "tags"=>["diap1", "coexpressed", "parvin-gfp", "dissorganized", "integrin-matrix", "adhesion"], "article_id"=>231242, "categories"=>["Molecular Biology", "Biological Sciences", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Maria Chountala", "Katerina M. Vakaloglou", "Christos G. Zervas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047355.g010", "stats"=>{"downloads"=>1, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ILK_and_DIAP1_coexpressed_with_Parvin_GFP_do_not_rescue_the_dissorganized_integrin_matrix_adhesion_sites_/231242", "title"=>"ILK and DIAP1 coexpressed with Parvin-GFP do not rescue the dissorganized integrin-matrix adhesion sites.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:54:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/560371"], "description"=>"<p>Confocal optical sections acquired apically (A–B), in the middle (C–D) and at the basal side of the epithelium (E–F) from wing imaginal discs with <i>enGal4</i> driving expression in the posterior compartment of <i>UAS::Parvin-GFP</i> alone (green, A, C, E; white, A′′, C′′, E′′) or coexpression with <i>UAS:ILK</i> and <i>UAS:DIAP1</i> (green, B, D, F; white B′′, D′′, F′′). Imaginal discs were probed with rhodamine-labelled phalloidin to visualize F-actin (red, A–F; white A′–F′) and DAPI to visualize nuclei (blue, A–F; white A′′′–F′′′). (G) A cross optical section of the imaginal disc appearing in images A, C, and E. (H) a cross optical section of the imaginal disc appearing in images B, D, and F. (I) Graphic cartoon based on the cross optical sections G and H. Small arrows, closed areas in the posterior and anterior compartment of the wing pouch expressing (right) or not expressing (left) <i>UAS::Parvin-GFP</i>; Arrowheads, areas within the posterior compartment that do not express <i>UAS::Parvin-GFP</i>; dashed arrows, disrupted F-actin; open arrowheads, pyknotic nuclei. Lines encircle the region of the posterior compartment retained expression of <i>UAS::Parvin-GFP</i>. The anterior part of the wing disc (where Parvin-GFP expression is not induced) serves as an internal control.</p>", "links"=>[], "tags"=>["overexpression", "imaginal", "epithelium", "disrupts", "basal", "f-actin"], "article_id"=>230859, "categories"=>["Molecular Biology", "Biological Sciences", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Maria Chountala", "Katerina M. Vakaloglou", "Christos G. Zervas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047355.g008", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Parvin_overexpression_in_the_wing_imaginal_disc_epithelium_disrupts_the_basal_F_actin_cytoskeleton_/230859", "title"=>"Parvin overexpression in the wing imaginal disc epithelium disrupts the basal F-actin cytoskeleton.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:52:10"}
  • {"files"=>["https://ndownloader.figshare.com/files/561709"], "description"=>"<p>Adult eye confocal optical projections from the <i>longGMRGal4</i> stock served as control (A), the starting <i>UAS::Parvin-GFP, longGMRGal4</i> genotype (B) and the recovered modifiers (C–L). The main suppressors of the modifier screen show morphology of the ommatidia that is reminiscent of the control: <i>UAS:Wech</i> (C), <i>UAS:PTEN</i> (E), <i>Df(3L)Exel6119</i> (G), <i>Df(3L)ED2</i> (I), and <i>UAS:IRXrp1</i> (K). The main enhancers of the screen are <i>Df(3R)e-GC3</i> (D), <i>UAS:IREip93F</i> (F), <i>UAS:IRmys</i> (H), <i>UAS:IRzasp52</i> (J), and <i>UAS:IRChd64</i> (L).</p>", "links"=>[], "tags"=>["phenotype", "enhancers", "suppressors"], "article_id"=>232206, "categories"=>["Molecular Biology", "Biological Sciences", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Maria Chountala", "Katerina M. Vakaloglou", "Christos G. Zervas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047355.g014", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Rough_eye_phenotype_and_identification_of_enhancers_and_suppressors_of_UAS_Parvin_GFP_longGMRGal4_/232206", "title"=>"Rough eye phenotype and identification of enhancers and suppressors of <i>UAS::Parvin-GFP, longGMRGal4</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:59:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/559350"], "description"=>"<p>Confocal projections of wing imaginal discs of late third instar larvae (A–D), and optical sections acquired at different focal planes as indicated (E1, E2, E3, E4–F1, F2, F3, F4), with <i>ptcGal4</i> driving expression of <i>UAS::Parvin-GFP</i> (A, C, F1–F4), or <i>UAS::Parvin<sup>ΔCH2</sup>-GFP</i> (B, E1–E4), or <i>UAS::Parvin-GFP</i> together with <i>UAS:IRParvin</i> (D). Imaginal wing discs expressing Parvin-GFP (green, A–F3; white, A′–D′, E1′′–F3′′) were probed for rhodamine-phalloidin to visualize F-actin (magenta, A–B, D, E1–E4, F1–F4; white, E1′–E3′, F1′–F3′) or MMP1 (magenta, C; white C′). (E4–F4) Cross optical sections of the imaginal discs appearing in images E1–F1 taken in the middle of the wing poutch.Graphic cartoon based on the optical sections shown in images E4 and F4 (G). Arrowheads indicate the expression domain of <i>ptcGal4</i> that drives expression of the transgene <i>UAS::Parvin<sup>ΔCH2</sup>-GFP</i>; large arrows point to migrating cells that have invaded the proximal areas of <i>UAS::Parvin-GFP</i> expression; dashed arrowheads denote small cellular debris containing pyknotic nuclei; and open arrows show areas lacking F-actin staining in the basal side of discs expressing <i>UAS::Parvin-GFP.</i> sc; stalk cells.</p>", "links"=>[], "tags"=>["overexpression", "epithelium", "leads", "mmp1", "secretion"], "article_id"=>229843, "categories"=>["Molecular Biology", "Biological Sciences", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Maria Chountala", "Katerina M. Vakaloglou", "Christos G. Zervas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047355.g004", "stats"=>{"downloads"=>1, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Parvin_overexpression_in_the_wing_epithelium_leads_to_cell_extrusion_MMP1_secretion_and_invasion_/229843", "title"=>"Parvin overexpression in the wing epithelium leads to cell extrusion, MMP1 secretion and invasion.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:46:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/561914"], "description"=>"<p>Truncated forms of <i>UAS::Parvin-GFP</i> expressed with specific <i>Gal4</i> drivers did not affect tissue morphogenesis.</p>", "links"=>[], "tags"=>["forms", "drivers", "did"], "article_id"=>232408, "categories"=>["Molecular Biology", "Biological Sciences", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Maria Chountala", "Katerina M. Vakaloglou", "Christos G. Zervas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047355.t002", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Truncated_forms_of_UAS_Parvin_GFP_expressed_with_specific_Gal4_drivers_did_not_affect_tissue_morphogenesis_/232408", "title"=>"Truncated forms of <i>UAS::Parvin-GFP</i> expressed with specific <i>Gal4</i> drivers did not affect tissue morphogenesis.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-19 22:00:47"}
  • {"files"=>["https://ndownloader.figshare.com/files/558977"], "description"=>"<p>(A–I) Wing imaginal discs of late third instar larvae from control <i>enGal4</i> (A–C), <i>enGal4/+;UAS::Parvin-GFP/+</i> (D–F), <i>enGal4/UAS:ILK;UAS::Parvin-GFP/UAS:DIAP1</i> (G–I). Wing discs expressed either only Gal4 (A–C), or Parvin-GFP (green, D–F), or ILK, DIAP1 and Parvin-GFP together (green, G–I), and were probed for activated caspase-3 (magenta, A, D, G; white A′, D′, G′), active p-JNK (magenta, B, E, H; white B′, E′, H′), or lacZ expressed from the <i>puc</i> locus (magenta, C, F, I; white C′, F′, I′). (D1–D1′) A cross optical section taken in the middle of the wing poutch from the imaginal disc appearing in image D. Arrowheads indicate apoptotic cells; open arrowheads indicate areas of active JNK; arrows, closed areas in the posterior and anterior compartment of the wing pouch expressing (right) or not expressing (left) <i>UAS::Parvin-GFP</i>; dashed arrows indicate stalk cells in the wing notum area. The anterior part of the wing disc (where Parvin-GFP expression is not induced) serves as an internal control. (K) Quantification of caspase-3 signal intensity in wing imaginal discs and the percentage of adult viability. (L1–L4) Adult wings derived from flies expressing only <i>enGal4</i> (L1), <i>UAS::Parvin-GFP</i> under <i>enGal4</i> (L2, L3) and <i>UAS:ILK</i> together with <i>UAS:DIAP1</i> and <i>UAS::Parvin-GFP</i> under <i>enGal4</i> (L4). Arrows indicate vein defects and notched areas in the wing.</p>", "links"=>[], "tags"=>["overexpression", "induces", "apoptosis", "activation", "jnk"], "article_id"=>229467, "categories"=>["Molecular Biology", "Biological Sciences", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Maria Chountala", "Katerina M. Vakaloglou", "Christos G. Zervas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047355.g002", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Parvin_overexpression_induces_apoptosis_and_activation_of_JNK_signaling_/229467", "title"=>"Parvin overexpression induces apoptosis and activation of JNK signaling.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:44:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/561942"], "description"=>"<p><b>Table key</b>. <b>E</b>: Enhancement; e: mild enhancement; <b>S</b>: Suppression; s: mild suppression. Modifiers phenotype when expressed under longGMR alone; mr: mild rough-eye, gl:glassy.</p>", "links"=>[], "tags"=>["modifiers", "cytogenetic", "deficiencies"], "article_id"=>232435, "categories"=>["Molecular Biology", "Biological Sciences", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Maria Chountala", "Katerina M. Vakaloglou", "Christos G. Zervas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047355.t003", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Summary_of_all_identified_modifiers_of_UAS_Parvin_GFP_longGMRGal4_in_the_developing_eye_including_information_on_the_stock_used_the_cytogenetic_map_for_the_deficiencies_and_the_effect_of_each_modifier_/232435", "title"=>"Summary of all identified modifiers of <i>UAS::Parvin-GFP, longGMRGal4</i> in the developing eye, including information on the stock used, the cytogenetic map for the deficiencies and the effect of each modifier.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-19 22:00:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/558666"], "description"=>"<p>Images were collected with a cooled CCD camera for various adult structures. Thoracic bristles in wild type (A) were missing upon expression of <i>UAS::Parvin-GFP</i> under <i>ptcGal4</i> (A′). Leg from a wild type adult fly (B) was malformed when <i>UAS::Parvin-GFP</i> was expressed under <i>ptcGal4</i> (B′). Ocellar bristles and arista from the head of a wild type adult fly (C) were missing upon <i>UAS::Parvin-GFP</i> expression under <i>ptcGal4</i> (C′). A compound eye from a wild type adult fly (D) took on a rough appearance when <i>UAS::Parvin-GFP</i> was expressed under <i>longGMRGal4</i> (D′). Arrows depict a wild type tissue structure, whereas dashed arrows indicate defects.</p>", "links"=>[], "tags"=>["parvin", "morphogenetic", "defects", "tissues"], "article_id"=>229157, "categories"=>["Molecular Biology", "Biological Sciences", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Maria Chountala", "Katerina M. Vakaloglou", "Christos G. Zervas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047355.g001", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Overexpression_of_Parvin_results_in_morphogenetic_defects_at_various_tissues_in_the_adult_fly_/229157", "title"=>"Overexpression of Parvin results in morphogenetic defects at various tissues in the adult fly.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:43:03"}
  • {"files"=>["https://ndownloader.figshare.com/files/561057"], "description"=>"<p>Confocal optical sections were acquired basally (A, C) or in the middle (B–D) from wing imaginal discs expressing <i>UAS::Parvin-GFP</i> alone (green, A, B; white, A′′, B′′) or with both <i>UAS:ILK</i> and <i>UAS:DIAP1</i> (green, C, D; white C′′, D′′), as driven by <i>enGal4</i> in the posterior compartment, probed for Rho1 (red, A–D; white A′–D′) and stained with DAPI to visualize nuclei (blue, A–D; white A′′′–D′′′). (E) Box-and-whisker plot of Rho1 levels indicating the means (vertical lines in the middle of the rectangular boxes) of measurements taken in apical, middle and basal focal planes. All individual measurements are superimposed on the box-and-whisker plots and are indicated by the same symbol in all focal planes to allow direct comparisons of the variation in pixel intensity. Arrows, closed areas in the posterior and anterior compartment of the wing pouch expressing (right) or not expressing (left) <i>UAS::Parvin-GFP</i>. The anterior part of the wing disc serves as an internal control.</p>", "links"=>[], "tags"=>["overexpression", "epithelium", "leads", "rho1"], "article_id"=>231548, "categories"=>["Molecular Biology", "Biological Sciences", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Maria Chountala", "Katerina M. Vakaloglou", "Christos G. Zervas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047355.g011", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Parvin_overexpression_in_the_wing_epithelium_leads_to_Rho1_elevation_mainly_basally_/231548", "title"=>"Parvin overexpression in the wing epithelium leads to Rho1 elevation, mainly basally.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:56:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/561870"], "description"=>"<p>Gal4 drivers used to direct expression of <i>UAS::Parvin-GFP</i> during <i>Drosophila</i> development.</p>", "links"=>[], "tags"=>["drivers"], "article_id"=>232369, "categories"=>["Molecular Biology", "Biological Sciences", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Maria Chountala", "Katerina M. Vakaloglou", "Christos G. Zervas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047355.t001", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Gal4_drivers_used_to_direct_expression_of_UAS_Parvin_GFP_during_Drosophila_development_/232369", "title"=>"Gal4 drivers used to direct expression of <i>UAS::Parvin-GFP</i> during <i>Drosophila</i> development.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-19 22:00:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/559830"], "description"=>"<p>Confocal optical sections were acquired at different focal planes (A, D) apically, (B, E) in the middle, (C, F) at the basal side of wing imaginal discs of late third instar larvae expressing <i>UAS::Parvin-GFP</i> (green, A–C; white, A′′–C′′) and probed with antibodies against Engrailed (red, A–C; white A′–C′) or coexpressing <i>UAS:ABDmoesin-RFP</i> (red, D–F; white D′–F′) and <i>UAS::Parvin-GFP</i> (green, D–F; white D′′–F′′) under <i>enGal4</i> in the posterior compartment of the disc. Arrows: areas in the posterior compartment where expression of <i>UAS::Parvin-GFP</i> is retained; dashed arrows: areas in the posterior compartment that have lost expression of <i>UAS::Parvin-GFP</i>; arrowheads: cells in the anterior compartment expressing <i>enGal4</i>; open arrowheads: areas in the posterior compartment expressing Engrailed; open arrows: areas in the posterior compartment expressing <i>UAS:ABDmoesin-RFP</i>.</p>", "links"=>[], "tags"=>["retained", "epithelial", "cells", "overexpressing"], "article_id"=>230323, "categories"=>["Molecular Biology", "Biological Sciences", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Maria Chountala", "Katerina M. Vakaloglou", "Christos G. Zervas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047355.g006", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Posterior_identity_is_retained_in_wing_epithelial_cells_overexpressing_Parvin_/230323", "title"=>"Posterior identity is retained in wing epithelial cells overexpressing Parvin.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:49:15"}
  • {"files"=>["https://ndownloader.figshare.com/files/561814"], "description"=>"<p>(A) The consequences of elevated levels of Parvin in the wing epithelium based on data from this and previous studies <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047355#pone.0047355-Neisch1\" target=\"_blank\">[25]</a>, <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047355#pone.0047355-Vidal1\" target=\"_blank\">[26]</a>. Parvin induces the upregulation of protein levels of Rho1 basally that leads to activation of the JNK pathway resulting in apoptosis. The abnormal levels of Rho1 could also affect the organization of F-actin leading to changes in cell shape, cell exclusion and cell invasion. Collectively these events can also enhance apoptosis and vice versa, as indicated by the double arrow. The coexpression of both ILK and DIAP1 suppresses the activation of DRONC and ameliorates cell exclusion. (B) The consequences of Parvin overexpression in the developing eye that lead to disruption of F-actin basally and the formation of a rough eye. This phenotype is suppressed or enhanced by overexpression or knockdown of specific genes.</p>", "links"=>[], "tags"=>["interactions", "parvin"], "article_id"=>232308, "categories"=>["Molecular Biology", "Biological Sciences", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Maria Chountala", "Katerina M. Vakaloglou", "Christos G. Zervas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047355.g015", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Model_of_the_genetic_interactions_of_Parvin_in_the_wing_and_eye_/232308", "title"=>"Model of the genetic interactions of Parvin in the wing and eye.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 22:00:14"}
  • {"files"=>["https://ndownloader.figshare.com/files/560542"], "description"=>"<p>Confocal optical sections were acquired apically (A–B), in the middle (C–D) and on the basal side of the epithelium (E–F) from wing imaginal discs with coexpression driven by <i>enGal4</i> in the posterior compartment of <i>UAS::Parvin-GFP</i> (green, A–F; white A–F′′) and <i>UAS:DIAP1</i> (A, C, E) or <i>UAS::Parvin-GFP</i> and <i>UAS:ILK</i> (B, D, F). Imaginal discs were probed with rhodamine-labelled phalloidin to visualize F-actin (red, A–F; white A′–F′).</p>", "links"=>[], "tags"=>["diap1", "individually", "coexpressed", "parvin", "ameliorate", "f-actin", "misorganization"], "article_id"=>231031, "categories"=>["Molecular Biology", "Biological Sciences", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Maria Chountala", "Katerina M. Vakaloglou", "Christos G. Zervas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047355.g009", "stats"=>{"downloads"=>3, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ILK_and_DIAP1_individually_coexpressed_with_Parvin_partially_ameliorate_F_actin_misorganization_in_the_wing_epithelium_/231031", "title"=>"ILK and DIAP1 individually coexpressed with Parvin partially ameliorate F-actin misorganization in the wing epithelium.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:53:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/559144"], "description"=>"<p>Confocal optical sections acquired from wing imaginal discs of late third instar larvae expressing <i>UAS::Parvin<sup>ΔCH2</sup>-GFP</i> (green, A), or coexpressing either <i>UAS:DIAP1</i> and <i>UAS::Parvin-GFP</i> (green, B) or <i>UAS:ILK</i> and <i>UAS::Parvin-GFP</i> (green, C), under <i>enGal4</i> in the posterior compartment of the disc, probed for activated caspase-3 (magenta, A–C; white A′–C′). Arrowheads indicate apoptotic cells. The anterior part of the wing disc (where Parvin-GFP expression is not induced) serves as an internal control. (D–E) Quantification of the caspase-3 signal intensity in wing imaginal discs (D) and the percentage of adult viability (E).</p>", "links"=>[], "tags"=>["parvin", "lacking", "ch2-domain", "coexpression", "ilk"], "article_id"=>229626, "categories"=>["Molecular Biology", "Biological Sciences", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Maria Chountala", "Katerina M. Vakaloglou", "Christos G. Zervas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047355.g003", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_of_Parvin_lacking_the_CH2_domain_and_Parvin_coexpression_with_ILK_or_DIAP1_/229626", "title"=>"Expression of Parvin lacking the CH2-domain and Parvin coexpression with ILK or DIAP1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:45:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/560097"], "description"=>"<p>Confocal optical sections acquired from wing imaginal discs of late third instar larvae expressing <i>UAS::Parvin-GFP</i> (green, A–D; white A′–D′) under <i>enGal4</i> in the posterior compartment of the disc, probed with an antibody against Parvin (red, A–D; white A′′–D′′) and DAPI to visualize the nuclei (blue, A–D; white A′′′–C′′′). (D) cross optical section taken in the middle of the wing poutch from the imaginal disc appearing in images A–C. Arrows: cells expressing Parvin detected either by the antibody or by Parvin-GFP; dashed arrows: cells where Parvin-GFP is undetectable; open arrowheads:pyknotic nuclei; open arrow: an area in the posterior compartment with high density of nuclei outlined with a light blue dashed line.</p>", "links"=>[], "tags"=>["anti-parvin", "parvin-gfp", "detection", "imaginal"], "article_id"=>230587, "categories"=>["Molecular Biology", "Biological Sciences", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Maria Chountala", "Katerina M. Vakaloglou", "Christos G. Zervas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047355.g007", "stats"=>{"downloads"=>4, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparison_of_anti_Parvin_and_Parvin_GFP_detection_in_the_wing_imaginal_discs_/230587", "title"=>"Comparison of anti-Parvin and Parvin-GFP detection in the wing imaginal discs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:50:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/561373"], "description"=>"<p>Confocal optical sections acquired basally (A), in the middle (B) or apically (C) of the wing imaginal discs with <i>enGal4</i> driving expression in the posterior compartment of <i>UAS::Parvin-GFP</i> (green, A–C; white, A′–C′) probed for the Rho1 effector Dia (red, A–C; white A′′–C′′) or DAPI to visualize the nuclei (blue, A–C; white A′′–C′′). Small arrows, closed areas in the posterior and anterior compartment of the wing pouch expressing (right) or not expressing (left) <i>UAS::Parvin-GFP</i>. The anterior part of the wing disc (where Parvin-GFP expression is not induced) serves as an internal control.</p>", "links"=>[], "tags"=>["overexpression", "endogenous", "levels"], "article_id"=>231868, "categories"=>["Molecular Biology", "Biological Sciences", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Maria Chountala", "Katerina M. Vakaloglou", "Christos G. Zervas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047355.g012", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Parvin_overexpression_does_not_affect_the_endogenous_levels_of_Dia_/231868", "title"=>"Parvin overexpression does not affect the endogenous levels of Dia.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:57:48"}

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Relative Metric

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