Astragalus Root and Elderberry Fruit Extracts Enhance the IFN-β Stimulatory Effects of Lactobacillus acidophilus in Murine-Derived Dendritic Cells
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{"title"=>"Astragalus Root and Elderberry Fruit Extracts Enhance the IFN-β Stimulatory Effects of Lactobacillus acidophilus in Murine-Derived Dendritic Cells", "type"=>"journal", "authors"=>[{"first_name"=>"Hanne", "last_name"=>"Frøkiær", "scopus_author_id"=>"6701788150"}, {"first_name"=>"Louise", "last_name"=>"Henningsen", "scopus_author_id"=>"55440786000"}, {"first_name"=>"Stine Broeng", "last_name"=>"Metzdorff", "scopus_author_id"=>"8621953700"}, {"first_name"=>"Gudrun", "last_name"=>"Weiss", "scopus_author_id"=>"36444412900"}, {"first_name"=>"Marc", "last_name"=>"Roller", "scopus_author_id"=>"36971509400"}, {"first_name"=>"John", "last_name"=>"Flanagan", "scopus_author_id"=>"56948289800"}, {"first_name"=>"Emilie", "last_name"=>"Fromentin", "scopus_author_id"=>"56671191500"}, {"first_name"=>"Alvin", "last_name"=>"Ibarra", "scopus_author_id"=>"34976654000"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84868132507", "sgr"=>"84868132507", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0047878", "pmid"=>"23118903", "pui"=>"365953525"}, "id"=>"2784b741-cbde-334f-88ae-86add8f5e96c", "abstract"=>"Many foods and food components boost the immune system, but little data are available regarding the mechanisms by which they do. Bacterial strains have disparate effects in stimulating the immune system. In dendritic cells, the gram-negative bacteria Escherichia coli upregulates proinflammatory cytokines, whereas gram-positive Lactobacillus acidophilus induces a robust interferon (IFN)-β response. The immune-modulating effects of astragalus root and elderberry fruit extracts were examined in bone marrow-derived murine dendritic cells that were stimulated with L. acidophilus or E. coli. IFN-β and other cytokines were measured by ELISA and RT-PCR. Endocytosis of fluorescence-labeled dextran and L. acidophilus in the presence of elderberry fruit or astragalus root extract was evaluated in dendritic cells. Our results show that both extracts enhanced L. acidophilus-induced IFN-β production and slightly decreased the proinflammatory response to E. coli. The enhanced IFN-β production was associated with upregulation of toll-like receptor 3 and to a varying degree, the cytokines IL-12, IL-6, IL-1β and TNF-α. Both extracts increased endocytosis in immature dendritic cells, and only slightly influenced the viability of the cells. In conclusion, astragalus root and elderberry fruit extracts increase the IFN-β inducing activity of L. acidophilus in dendritic cells, suggesting that they may exert antiviral and immune-enhancing activity.", "link"=>"http://www.mendeley.com/research/astragalus-root-elderberry-fruit-extracts-enhance-ifn%CE%B2-stimulatory-effects-lactobacillus-acidophilus", "reader_count"=>28, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Researcher"=>7, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>4, "Student > Postgraduate"=>1, "Other"=>2, "Student > Master"=>3, "Student > Bachelor"=>6}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Researcher"=>7, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>4, "Student > Postgraduate"=>1, "Other"=>2, "Student > Master"=>3, "Student > Bachelor"=>6}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>14, "Medicine and Dentistry"=>4, "Chemistry"=>4, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Chemistry"=>{"Chemistry"=>4}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>14}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>3}}, "reader_count_by_country"=>{"Mexico"=>1, "United Kingdom"=>1, "India"=>1}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/551162"], "description"=>"<p>Astragalus root (A, C, E) and elderberry fruit (B, D, F) extracts differentially modulate TNF-α (A, B), IL-1β (C, D), and IL-6 (E, F) production when added to murine bone marrow-derived dendritic cells at concentrations from 15 to 500 µg/mL 30 min prior to the addition <i>L. acidophilus</i> (10 µg/mL) or <i>E. coli</i> (10 µg/mL). Supernatants were harvested for ELISA after 20 hr incubation. The results are representative of at least 3 experiments. Error bars are representative of the LSD intervals. The difference between 2 groups was considered significant when the LSD intervals did not overlap (p-value<0.05).</p>", "links"=>[], "tags"=>["elderberry", "extracts", "modulate", "il-6", "dendritic"], "article_id"=>221645, "categories"=>["Microbiology", "Immunology", "Plant Biology"], "users"=>["Hanne Frøkiær", "Louise Henningsen", "Stine Broeng Metzdorff", "Gudrun Weiss", "Marc Roller", "John Flanagan", "Emilie Fromentin", "Alvin Ibarra"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047878.g002", "stats"=>{"downloads"=>1, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Astragalus_and_elderberry_extracts_modulate_TNF_945_IL_1_946_and_IL_6_in_dendritic_cells_/221645", "title"=>"Astragalus and elderberry extracts modulate TNF-α, IL-1β, and IL-6 in dendritic cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:03:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/551057"], "description"=>"<p>Astragalus root (A, C) and elderberry fruit (B, D) extracts enhance IL-12 (A, B) and IL-10 (C, D) production when added to murine bone marrow-derived dendritic cells at concentrations from 15 to 500 µg/mL 30 min prior to stimulation with <i>L. acidophilus</i> (10 µg/mL) but not with <i>E. coli</i> (10 µg/mL). Supernatants were harvested for ELISA after 20 hr incubation. The results are representative of at least 3 experiments. Error bars are representative of the LSD intervals. The difference between 2 groups was considered significant when the LSD intervals did not overlap (p-value<0.05).</p>", "links"=>[], "tags"=>["elderberry", "extracts", "modulate", "il-12", "il10", "dendritic"], "article_id"=>221534, "categories"=>["Microbiology", "Immunology", "Plant Biology"], "users"=>["Hanne Frøkiær", "Louise Henningsen", "Stine Broeng Metzdorff", "Gudrun Weiss", "Marc Roller", "John Flanagan", "Emilie Fromentin", "Alvin Ibarra"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047878.g001", "stats"=>{"downloads"=>1, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Astragalus_and_elderberry_extracts_modulate_IL_12_and_IL10_production_in_dendritic_cells_/221534", "title"=>"Astragalus and elderberry extracts modulate IL-12 and IL10 production in dendritic cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:02:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/551279"], "description"=>"<p>DCs were incubated with increasing concentrations of the astragalus extract (A) and elderberry extract (B) and left unstimulated or stimulated with <i>E.coli</i> Nissle (10 µg/mL) or <i>L.acidophilus</i> NCFM (10 µg/mL). After 20 hr the cells were harvested and stained for viability with 7AAD for 15 min., washed and analysed by flow cytometry. (*: p<0.05; **: p<0.01; ***: p<0.001).</p>", "links"=>[], "tags"=>["elderberry", "extracts", "viability"], "article_id"=>221769, "categories"=>["Microbiology", "Immunology", "Plant Biology"], "users"=>["Hanne Frøkiær", "Louise Henningsen", "Stine Broeng Metzdorff", "Gudrun Weiss", "Marc Roller", "John Flanagan", "Emilie Fromentin", "Alvin Ibarra"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047878.g003", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Astragalus_root_and_Elderberry_extracts_only_slightly_affect_the_viability_of_DCs_/221769", "title"=>"Astragalus root and Elderberry extracts only slightly affect the viability of DCs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:03:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/551345"], "description"=>"<p>Extracts of astragalus root (A) and elderberry fruit (B) were added to murine bone marrow-derived dendritic cells at the concentrations 15 and 150 µg/mL prior to the addition of <i>L. acidophilus</i> (10 µg/mL) and incubated at 2, 4, 10, and 20 hr. Supernatants from the cells that were incubated for 20 hr were harvested, and IFN-β was measured by ELISA. Error bars are representative of the LSD intervals. The difference between 2 groups was considered significant when the LSD intervals did not overlap (p-value<0.05).</p>", "links"=>[], "tags"=>["elderberry", "extracts", "potentiate", "dendritic"], "article_id"=>221830, "categories"=>["Microbiology", "Immunology", "Plant Biology"], "users"=>["Hanne Frøkiær", "Louise Henningsen", "Stine Broeng Metzdorff", "Gudrun Weiss", "Marc Roller", "John Flanagan", "Emilie Fromentin", "Alvin Ibarra"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047878.g004", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Astragalus_and_elderberry_extracts_potentiate_the_IFN_inducing_capacity_of_L_acidophilus_in_dendritic_cells_/221830", "title"=>"Astragalus and elderberry extracts potentiate the IFN-β-inducing capacity of <i>L. acidophilus</i> in dendritic cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:04:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/551450"], "description"=>"<p>Extract of elderberry fruit was added to murine bone marrow-derived dendritic cells at the concentration 500 µg/mL prior to the addition of <i>L. acidophilus</i> (10 µg/mL) and incubated at 2, 4, 10, and 20 hr. RNA was harvested at various time points and analyzed for <i>Ifnβ</i>, (A), <i>Tlr3</i> (B), <i>Il12</i> (C) and <i>Il10</i> (D) expression by RT-PCR relative to β-actin. Cells only stimulated with <i>L.acidophilus</i> or elderberry extract were used as controls. Error bars are representative of the LSD intervals. The difference between 2 groups was considered significant when the LSD intervals did not overlap (p-value<0.05).</p>", "links"=>[], "tags"=>["extract", "potentiates", "dendritic"], "article_id"=>221937, "categories"=>["Microbiology", "Immunology", "Plant Biology"], "users"=>["Hanne Frøkiær", "Louise Henningsen", "Stine Broeng Metzdorff", "Gudrun Weiss", "Marc Roller", "John Flanagan", "Emilie Fromentin", "Alvin Ibarra"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047878.g005", "stats"=>{"downloads"=>0, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Elderberry_extract_potentiates_Ifn_946_Trl3_Il10_and_Il12_gene_expression_by_L_acidophilus_in_dendritic_cells_/221937", "title"=>"Elderberry extract potentiates <i>Ifn-β, Trl3, Il10</i> and <i>Il12</i> gene expression by <i>L. acidophilus</i> in dendritic cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:04:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/551738"], "description"=>"<p>Real-Time PCR primers and probes.</p>", "links"=>[], "tags"=>["pcr", "primers"], "article_id"=>222230, "categories"=>["Microbiology", "Immunology", "Plant Biology"], "users"=>["Hanne Frøkiær", "Louise Henningsen", "Stine Broeng Metzdorff", "Gudrun Weiss", "Marc Roller", "John Flanagan", "Emilie Fromentin", "Alvin Ibarra"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047878.t001", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Real_Time_PCR_primers_and_probes_/222230", "title"=>"Real-Time PCR primers and probes.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-19 21:06:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/551571"], "description"=>"<p>Immature murine bone marrow-derived dendritic cells were incubated with elderberry fruit extract, astragalus root extract, or LPS for 30 min prior to the addition of FITC-labeled 10 kD-dextran molecules (A, B) or Alexa Fluor 647-labeled <i>L. acidophilus</i> (C, D). <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047878#s3\" target=\"_blank\">Results</a> are expressed as % of fluorescent cells (A, C) and as mean fluorescence intensity (MFI) (B, D). Error bars are representative of the LSD intervals. The difference between 2 groups was considered significant when the LSD intervals did not overlap (p-value<0.05). Lower panel show examples of histograms of which A–B were made; Fitc-dextran (left) or Alexa Fluor 647-conjugated L.acidophilus (right) incubated cells with or without prior incubation with elderberry fruit extract.</p>", "links"=>[], "tags"=>["astragalus", "dextran", "uptake", "immature", "dendritic"], "article_id"=>222054, "categories"=>["Microbiology", "Immunology", "Plant Biology"], "users"=>["Hanne Frøkiær", "Louise Henningsen", "Stine Broeng Metzdorff", "Gudrun Weiss", "Marc Roller", "John Flanagan", "Emilie Fromentin", "Alvin Ibarra"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0047878.g006", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Elderberry_and_astragalus_increase_dextran_and_L_acidophilus_uptake_in_immature_dendritic_cells_/222054", "title"=>"Elderberry and astragalus increase dextran and <i>L. acidophilus</i> uptake in immature dendritic cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 21:05:08"}

PMC Usage Stats | Further Information

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Relative Metric

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