Extracellular DNA Release by Undomesticated Bacillus subtilis Is Regulated by Early Competence
Publication Date
November 02, 2012
Journal
PLOS ONE
Authors
Olga Zafra, María Lamprecht Grandío, Carolina González De Figueras & José Eduardo González Pastor
Volume
7
Issue
11
Pages
e48716
DOI
https://dx.plos.org/10.1371/journal.pone.0048716
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0048716
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/23133654
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3487849
Europe PMC
http://europepmc.org/abstract/MED/23133654
Web of Science
000310702400044
Scopus
84868319858
Mendeley
http://www.mendeley.com/research/extracellular-dna-release-undomesticated-bacillus-subtilis-regulated-early-competence
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Mendeley | Further Information

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Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/549421"], "description"=>"<p>A. Differences in colony morphology between 3610 (left) and SPR, a spontaneous spread mutant (GP305, right) growing on LB. The picture in the middle corresponds to a 3610 colony with two branches of spread mutants (arrow). Bar represents 1 cm. B. eDNA production of wild type (3610) and several isogenic spread mutants: SPR-1 (GP305), SPR-2 (GP306), SPR-3 (GP307) and SPR-4 (GP319). Strains were grown in MSgg at 37°C with aeration (growth was similar for all the strains). eDNA refers to the eDNA concentration in the culture supernatant. Data presented are representative of results obtained in two independent experiments.</p>", "links"=>[], "tags"=>["phenotype", "3610", "spontaneous"], "article_id"=>219917, "categories"=>["Molecular Biology", "Cell Biology", "Genetics", "Microbiology"], "users"=>["Olga Zafra", "María Lamprecht-Grandío", "Carolina González de Figueras", "José Eduardo González-Pastor"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0048716.g004", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Spread_colony_phenotype_of_3610_spontaneous_mutants_/219917", "title"=>"Spread colony phenotype of 3610 spontaneous mutants.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-11-02 02:45:17"}
  • {"files"=>["https://ndownloader.figshare.com/files/549244"], "description"=>"<p>The percentage of extracellular β-galactosidase was measured in cultures of 3610 <i>amyE</i>::P<i>feuA</i>-<i>lacZ</i> (EG385). The strain was grown in liquid MSgg at 37°C with aeration. β-gal refers to the percentage of β-galactosidase activity outside the cell and eDNA refers to the eDNA concentration in the culture supernatant. Data presented are representative of results obtained in, at least, three independent experiments.</p>", "links"=>[], "tags"=>["3610", "caused"], "article_id"=>219738, "categories"=>["Molecular Biology", "Cell Biology", "Genetics", "Microbiology"], "users"=>["Olga Zafra", "María Lamprecht-Grandío", "Carolina González de Figueras", "José Eduardo González-Pastor"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0048716.g002", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_eDNA_production_in_B_subtilis_3610_is_not_caused_by_lysis_/219738", "title"=>"eDNA production in <i>B. subtilis</i> 3610 is not caused by lysis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-11-02 02:42:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/549572"], "description"=>"<p>A. Graphs show the overlapping of the DAPI intensity profiles of the wild type 3610 (light green) and the <i>degU</i>::mini-Tn<i>10</i> mutant (GP229) (dark blue), at several time points during growth. B. Upper side graph represents eDNA levels of 3610 and <i>degU</i>::mini-Tn<i>10</i> mutant. In the lower graph, bars represent percentage of cells with DAPI intensity higher than the mean in <i>B. subtilis</i> 3610 and <i>degU</i>::mini-Tn<i>10</i> mutant (GP229). Only one representative experiment is shown of two independent experiments. Growth was similar in both cases.</p>", "links"=>[], "tags"=>["cytometry"], "article_id"=>220067, "categories"=>["Molecular Biology", "Cell Biology", "Genetics", "Microbiology"], "users"=>["Olga Zafra", "María Lamprecht-Grandío", "Carolina González de Figueras", "José Eduardo González-Pastor"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0048716.g006", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Flow_cytometry_analysis_/220067", "title"=>"Flow cytometry analysis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-11-02 00:01:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/549301"], "description"=>"<p>eDNA was labelled with Cy3 (F532) and genomic DNA with Cy5 (F635) and hybridized on an oligonucleotide-based microarray of <i>B. subtilis</i> 168.</p>", "links"=>[], "tags"=>["corresponds", "genome"], "article_id"=>219796, "categories"=>["Molecular Biology", "Cell Biology", "Genetics", "Microbiology"], "users"=>["Olga Zafra", "María Lamprecht-Grandío", "Carolina González de Figueras", "José Eduardo González-Pastor"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0048716.g003", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_eDNA_corresponds_with_the_complete_genome_of_B_subtilis_/219796", "title"=>"eDNA corresponds with the complete genome of <i>B. subtilis</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-11-02 02:43:16"}
  • {"files"=>["https://ndownloader.figshare.com/files/549647"], "description"=>"<p>A. Competence development and eDNA production during <i>B. subtilis</i> 3610 growth in MSgg medium, 37°C. B. Transformation of eDNA from culture supernatants in late exponential phase in <i>B. subtilis</i> PY79. “skf/sdp” stands for the cannibalism mutant (GP315), degU is the <i>degU</i>::mini-Tn<i>10</i> mutant strain (GP229) and “3610feu” stands for <i>amy</i>E::P<i>feu</i>-<i>lac</i>Z strain (EG385). One representative experiment of two is shown.</p>", "links"=>[], "tags"=>["edna", "horizontal"], "article_id"=>220145, "categories"=>["Molecular Biology", "Cell Biology", "Genetics", "Microbiology"], "users"=>["Olga Zafra", "María Lamprecht-Grandío", "Carolina González de Figueras", "José Eduardo González-Pastor"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0048716.g007", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Role_of_the_eDNA_in_horizontal_gene_transfer_/220145", "title"=>"Role of the eDNA in horizontal gene transfer.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-11-02 00:02:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/549166"], "description"=>"<p>A. Batch culture of strain 3610 in MSgg at 37°C with aeration. A600 refers to the absorbance of the culture at 600 nm, and eDNA refers to the eDNA concentration in the culture supernatant. Data presented are representative of results obtained in, at least, three independent experiments. B. eDNA from another culture of strain 3610 was electrophorized on an agarose gel, the same volume of precipitated eDNA was loaded in each well.</p>", "links"=>[], "tags"=>["genetics and genomics", "microbiology", "molecular biology", "cell biology"], "article_id"=>219659, "categories"=>["Molecular Biology", "Cell Biology", "Genetics", "Microbiology"], "users"=>["Olga Zafra", "María Lamprecht-Grandío", "Carolina González de Figueras", "José Eduardo González-Pastor"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0048716.g001", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_eDNA_production_in_B_subtilis_3610_/219659", "title"=>"eDNA production in <i>B. subtilis</i> 3610.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-11-02 02:40:59"}
  • {"files"=>["https://ndownloader.figshare.com/files/549501"], "description"=>"<p>A. The graph shows the eDNA concentration on the culture supernatants of wild type 3610 and the mutants <i>oppA::</i>mini<i>-</i>Tn<i>10</i> (GP233), a insertional mutant in <i>opp</i>A; Δ<i>oppA</i>::<i>erm</i> (GP308), a deletion mutant in <i>opp</i>A; and the deletion mutant Δ<i>phrC</i> (GP236); (growth was similar for all the strains). B. Growth and eDNA levels of <i>com</i>A mutant (GP240) compared with wild type 3610.</p>", "links"=>[], "tags"=>["edna"], "article_id"=>219996, "categories"=>["Molecular Biology", "Cell Biology", "Genetics", "Microbiology"], "users"=>["Olga Zafra", "María Lamprecht-Grandío", "Carolina González de Figueras", "José Eduardo González-Pastor"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0048716.g005", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_oppA_phrC_and_comA_in_eDNA_production_/219996", "title"=>"Effect of <i>oppA</i>, <i>phrC</i> and <i>comA</i> in eDNA production.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-11-02 02:46:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/549756"], "description"=>"<p>Strains used in this work.</p>", "links"=>[], "tags"=>["genetics and genomics", "microbiology", "molecular biology", "cell biology"], "article_id"=>220247, "categories"=>["Molecular Biology", "Cell Biology", "Genetics", "Microbiology"], "users"=>["Olga Zafra", "María Lamprecht-Grandío", "Carolina González de Figueras", "José Eduardo González-Pastor"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0048716.t001", "stats"=>{"downloads"=>0, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Strains_used_in_this_work_/220247", "title"=>"Strains used in this work.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-11-02 00:04:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/295135", "https://ndownloader.figshare.com/files/295187", "https://ndownloader.figshare.com/files/295220", "https://ndownloader.figshare.com/files/295262", "https://ndownloader.figshare.com/files/295293", "https://ndownloader.figshare.com/files/295328", "https://ndownloader.figshare.com/files/295374", "https://ndownloader.figshare.com/files/295420", "https://ndownloader.figshare.com/files/295447"], "description"=>"<div><p>Extracellular DNA (eDNA) release is a widespread capacity described in many microorganisms. We identified and characterized lysis-independent eDNA production in an undomesticated strain of <em>Bacillus subtilis</em>. DNA fragments are released during a short time in late-exponential phase. The released eDNA corresponds to whole genome DNA, and does not harbour mutations suggesting that is not the result of error prone DNA synthesis. The absence of eDNA was linked to a spread colony morphology, which allowed a visual screening of a transposon library to search for genes involved in its production. Transposon insertions in genes related to quorum sensing and competence (<em>oppA</em>, <em>oppF</em> and <em>comXP</em>) and to DNA metabolism (<em>mfd</em> and <em>topA</em>) were impaired in eDNA release. Mutants in early competence genes such as <em>comA</em> and <em>srfAA</em> were also defective in eDNA while in contrast mutations in late competence genes as those for the DNA uptake machinery had no effect. A subpopulation of cells containing more DNA is present in the eDNA producing strains but absent from the eDNA defective strain. Finally, competent <em>B. subtilis</em> cells can be transformed by eDNA suggesting it could be used in horizontal gene transfer and providing a rationale for the molecular link between eDNA release and early-competence in <em>B. subtilis</em> that we report.</p> </div>", "links"=>[], "tags"=>["extracellular", "dna", "undomesticated", "regulated", "competence"], "article_id"=>118134, "categories"=>["Molecular Biology", "Cell Biology", "Genetics", "Microbiology"], "users"=>["Olga Zafra", "María Lamprecht-Grandío", "Carolina González de Figueras", "José Eduardo González-Pastor"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0048716.s001", "https://dx.doi.org/10.1371/journal.pone.0048716.s002", "https://dx.doi.org/10.1371/journal.pone.0048716.s003", "https://dx.doi.org/10.1371/journal.pone.0048716.s004", "https://dx.doi.org/10.1371/journal.pone.0048716.s005", "https://dx.doi.org/10.1371/journal.pone.0048716.s006", "https://dx.doi.org/10.1371/journal.pone.0048716.s007", "https://dx.doi.org/10.1371/journal.pone.0048716.s008", "https://dx.doi.org/10.1371/journal.pone.0048716.s009"], "stats"=>{"downloads"=>5, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Extracellular_DNA_Release_by_Undomesticated_Bacillus_subtilis_Is_Regulated_by_Early_Competence__/118134", "title"=>"Extracellular DNA Release by Undomesticated <em>Bacillus subtilis</em> Is Regulated by Early Competence", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-11-02 02:15:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/549721"], "description"=>"<p>Genes involved in eDNA release identified by the transposon library mutagenesis.</p>", "links"=>[], "tags"=>["edna", "transposon"], "article_id"=>220214, "categories"=>["Molecular Biology", "Cell Biology", "Genetics", "Microbiology"], "users"=>["Olga Zafra", "María Lamprecht-Grandío", "Carolina González de Figueras", "José Eduardo González-Pastor"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0048716.t002", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Genes_involved_in_eDNA_release_identified_by_the_transposon_library_mutagenesis_/220214", "title"=>"Genes involved in eDNA release identified by the transposon library mutagenesis.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-11-02 00:03:34"}

PMC Usage Stats | Further Information

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Relative Metric

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