ERMO3/MVP1/GOLD36 Is Involved in a Cell Type-Specific Mechanism for Maintaining ER Morphology in Arabidopsis thaliana
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{"title"=>"ERMO3/MVP1/GOLD36 Is Involved in a Cell Type-Specific Mechanism for Maintaining ER Morphology in Arabidopsis thaliana", "type"=>"journal", "authors"=>[{"first_name"=>"Ryohei Thomas", "last_name"=>"Nakano", "scopus_author_id"=>"56356546600"}, {"first_name"=>"Ryo", "last_name"=>"Matsushima", "scopus_author_id"=>"7006266526"}, {"first_name"=>"Atsushi J.", "last_name"=>"Nagano", "scopus_author_id"=>"35305206700"}, {"first_name"=>"Yoichiro", "last_name"=>"Fukao", "scopus_author_id"=>"16063131200"}, {"first_name"=>"Masayuki", "last_name"=>"Fujiwara", "scopus_author_id"=>"35236676600"}, {"first_name"=>"Maki", "last_name"=>"Kondo", "scopus_author_id"=>"7403405029"}, {"first_name"=>"Mikio", "last_name"=>"Nishimura", "scopus_author_id"=>"7403651506"}, {"first_name"=>"Ikuko", "last_name"=>"Hara-Nishimura", "scopus_author_id"=>"7006355054"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84869101190", "sgr"=>"84869101190", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0049103", "pmid"=>"23155454", "isbn"=>"1932-6203", "pui"=>"366053626"}, "id"=>"ba3dd9cc-211d-3b19-bb4a-88f7c442a656", "abstract"=>"The endoplasmic reticulum (ER) has a unique, network-like morphology. The ER structures are composed of tubules, cisternae, and three-way junctions. This morphology is highly conserved among eukaryotes, but the molecular mechanism that maintains ER morphology has not yet been elucidated. In addition, certain Brassicaceae plants develop a unique ER-derived organelle called the ER body. This organelle accumulates large amounts of PYK10, a β-glucosidase, but its physiological functions are still obscure. We aimed to identify a novel factor required for maintaining the morphology of the ER, including ER bodies, and employed a forward-genetic approach using transgenic Arabidopsis thaliana (GFP-h) with fluorescently-labeled ER. We isolated and investigated a mutant (designated endoplasmic reticulum morphology3, ermo3) with huge aggregates and abnormal punctate structures of ER. ERMO3 encodes a GDSL-lipase/esterase family protein, also known as MVP1. Here, we showed that, although ERMO3/MVP1/GOLD36 was expressed ubiquitously, the morphological defects of ermo3 were specifically seen in a certain type of cells where ER bodies developed. Coimmunoprecipitation analysis combined with mass spectrometry revealed that ERMO3/MVP1/GOLD36 interacts with the PYK10 complex, a huge protein complex that is thought to be important for ER body-related defense systems. We also found that the depletion of transcription factor NAI1, a master regulator for ER body formation, suppressed the formation of ER-aggregates in ermo3 cells, suggesting that NAI1 expression plays an important role in the abnormal aggregation of ER. Our results suggest that ERMO3/MVP1/GOLD36 is required for preventing ER and other organelles from abnormal aggregation and for maintaining proper ER morphology in a coordinated manner with NAI1.", "link"=>"http://www.mendeley.com/research/ermo3mvp1gold36-involved-cell-typespecific-mechanism-maintaining-er-morphology-arabidopsis-thaliana", "reader_count"=>23, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>3, "Researcher"=>10, "Student > Ph. D. Student"=>5, "Student > Master"=>3, "Student > Bachelor"=>1, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>3, "Researcher"=>10, "Student > Ph. D. Student"=>5, "Student > Master"=>3, "Student > Bachelor"=>1, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>3, "Mathematics"=>1, "Agricultural and Biological Sciences"=>19}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>19}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Mathematics"=>{"Mathematics"=>1}}, "reader_count_by_country"=>{"United States"=>1, "France"=>1}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/542515"], "description"=>"<p>(<b>A</b>) Transgenic <i>Arabidopsis</i> (GFP-h) expressing SP-GFP-HDEL was observed under a confocal microscope (left panels, WT). <i>ermo3-1</i> was isolated from chemically mutagenized GFP-h seeds and subjected to confocal microscopy (right panels). In GFP-h cells, the typical ER network (peripheral, inset) and ER bodies (medial, arrows) were seen. In <i>ermo3-1</i> cells, punctate structures (peripheral, arrowheads in inset) and huge aggregates (medial, open arrows) were seen in addition to the typical network and ER bodies. Bars, 10 µm. (<b>B</b>) Epidermal cells of petioles, hypocotyls, roots, and root hairs from 7-day-old <i>ermo3-1</i> developed ER-aggregates, as seen in cotyledons. Bars, 10 µm. (<b>C</b>) Transmission electron micrographs of GFP-h (<b>a</b>) and <i>ermo3-1</i> (<b>b</b>) cells. ER was usually seen as a thin structure in the periphery surrounded by ribosomes (arrowheads). ER bodies were recognized as large and relatively electron-dense compartments (inset). The <i>ermo3-1</i> aggregates appeared as huge structures consisting of various structures. Abnormal swollen structures were occasionally surrounded by ribosomes (arrowheads) and connected with ER cisternae (insets). Eb, ER body; V, vacuole; G, Golgi body; N, nucleus; Mt, mitochondrion; Per, peroxisome; Cw, cell wall; Pt, plastid. Bars, 1 µm (upper panel of [<b>a</b>] and left column of [<b>b</b>]) and 200 nm (lower panel of [<b>a</b>] and right column of [<b>b</b>]).</p>", "links"=>[], "tags"=>["developed", "aggregate", "composed", "er-derived", "aberrant", "structures"], "article_id"=>212994, "categories"=>["Cell Biology", "Genetics", "Plant Biology"], "users"=>["Ryohei Thomas Nakano", "Ryo Matsushima", "Atsushi J. Nagano", "Yoichiro Fukao", "Masayuki Fujiwara", "Maki Kondo", "Mikio Nishimura", "Ikuko Hara-Nishimura"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0049103.g001", "stats"=>{"downloads"=>2, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ermo3_1_developed_a_large_aggregate_composed_of_ER_derived_aberrant_structures_and_various_organelles_/212994", "title"=>"<i>ermo3-1</i> developed a large aggregate composed of ER-derived aberrant structures and various organelles.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-11-14 00:49:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/543216"], "description"=>"<p>(<b>A</b>) Rosette leaves from 3-week-old plants were treated with 45 µM of methyl jasmonate (MeJA), or water as a solvent control, for the indicated number of days. 3D-reconstructed images are shown. MeJA treatment induced ER bodies in both GFP-h and <i>ermo3-1</i> cells (arrows). Notably, MeJA treatment co-induced ER-aggregate formation in <i>ermo3-1</i> cells (open arrows). (<b>B</b>) Higher magnification views of 4 day-treated <i>ermo3-1</i> cells are shown. Arrowheads indicate tangled ER, which might act as a “seed” of the ER-aggregates. Bars, 50 µm.</p>", "links"=>[], "tags"=>["er", "meja", "induced", "er-aggregate", "rosette"], "article_id"=>213700, "categories"=>["Cell Biology", "Genetics", "Plant Biology"], "users"=>["Ryohei Thomas Nakano", "Ryo Matsushima", "Atsushi J. Nagano", "Yoichiro Fukao", "Masayuki Fujiwara", "Maki Kondo", "Mikio Nishimura", "Ikuko Hara-Nishimura"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0049103.g007", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Induction_of_ER_body_formation_by_MeJA_also_induced_ER_aggregate_in_ermo3_1_rosette_leaves_/213700", "title"=>"Induction of ER body formation by MeJA also induced ER-aggregate in <i>ermo3-1</i> rosette leaves.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-11-14 01:01:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/542644"], "description"=>"<p>(<b>A</b>) Confocal images obtained from T1 plants of <i>ermo3-1</i> transformed by <i>tdTomato-ABD2</i>. Panels in the middle row are enlarged views of top panels. The aggregates were surrounded by organized actin filaments. Bottom row images show that the peripheral actin network was properly organized. Bars, 10 µm. (<b>B</b>) Seedlings of GFP-h and <i>ermo3-1</i> were treated with 100 µM of Latrunculin B (Lat B), an actin inhibitor, for 2 hours. Green arrowheads indicate Lat B-induced aggregates, which were derived from collapsed ER networks. In the presence of Lat B, <i>ermo3</i> aggregates (open arrows) were still similar to those of non-treated cells. Bars, 10 µm.</p>", "links"=>[], "tags"=>["filaments"], "article_id"=>213139, "categories"=>["Cell Biology", "Genetics", "Plant Biology"], "users"=>["Ryohei Thomas Nakano", "Ryo Matsushima", "Atsushi J. Nagano", "Yoichiro Fukao", "Masayuki Fujiwara", "Maki Kondo", "Mikio Nishimura", "Ikuko Hara-Nishimura"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0049103.g002", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Actin_filaments_were_properly_organized_in_ermo3_1_cells_/213139", "title"=>"Actin filaments were properly organized in <i>ermo3-1</i> cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-11-14 00:52:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/542763"], "description"=>"<p>(<b>A</b>) Gene model and protein structure of At1g54030. Base-pair substitution found in <i>ermo3-1</i> and T-DNA insertion sites of <i>ermo3</i>-2 (SALK_135215) and <i>mvp1-2</i> (SALK_030621) are shown. Blue boxes and black lines indicate exons and introns, respectively. Conserved domains for catalytic triad and oxyanion holes are indicated by orange boxes. Φ indicates a putative glycosylation site. (<b>B</b>) RT-PCR using total RNA extracted from 7-day-old seedlings of GFP-h and <i>ermo3-1</i> amplified full-length ERMO3. <i>ermo3-1</i> transcribed two distinct mRNAs. <i>ACT1</i> was used for the internal control. (<b>C</b>) Sequencing results of mRNA transcribed in GFP-h and <i>ermo3-1</i> around the substitution site. Predicted protein sequences are also shown. Numbers above sequences indicate positions in CDS. #1 and #2 of <i>ermo3-1</i> correspond to shorter and longer mRNAs shown in (<b>B</b>), respectively. (<b>D</b>) Quantitative real-time PCR (qPCR) using total RNA from 7-day-old seedlings of CS60000, <i>ermo3</i>-2, and <i>mvp1-2</i>. The amplified region is indicated by a red bar in (<b>A</b>). <i>ERMO3</i> expression in <i>ermo3-2</i> was not detected and little in <i>mvp1-2</i> (*** <i>P</i><0.0001). (<b>E</b>) Total RNA from seedlings (7-day-old), roots (2-week-old), and aerial tissues (2-week-old) of GFP-h and <i>ermo3-2</i> were subjected to qPCR. <i>ERMO3</i> expression was detected in all three tissues of Col-0, while none was detected in <i>ermo3-2</i>. * <i>P</i><0.01, ** <i>P</i><0.001, *** <i>P</i><0.0001.</p>", "links"=>[], "tags"=>["genetics and genomics", "plant biology", "cell biology"], "article_id"=>213255, "categories"=>["Cell Biology", "Genetics", "Plant Biology"], "users"=>["Ryohei Thomas Nakano", "Ryo Matsushima", "Atsushi J. Nagano", "Yoichiro Fukao", "Masayuki Fujiwara", "Maki Kondo", "Mikio Nishimura", "Ikuko Hara-Nishimura"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0049103.g003", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Characterization_of_ERMO3_gene_/213255", "title"=>"Characterization of <i>ERMO3</i> gene.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-11-14 00:54:15"}
  • {"files"=>["https://ndownloader.figshare.com/files/291983", "https://ndownloader.figshare.com/files/292037", "https://ndownloader.figshare.com/files/292132", "https://ndownloader.figshare.com/files/292255", "https://ndownloader.figshare.com/files/292326", "https://ndownloader.figshare.com/files/292397"], "description"=>"<div><p>The endoplasmic reticulum (ER) has a unique, network-like morphology. The ER structures are composed of tubules, cisternae, and three-way junctions. This morphology is highly conserved among eukaryotes, but the molecular mechanism that maintains ER morphology has not yet been elucidated. In addition, certain <em>Brassicaceae</em> plants develop a unique ER-derived organelle called the ER body. This organelle accumulates large amounts of PYK10, a β-glucosidase, but its physiological functions are still obscure. We aimed to identify a novel factor required for maintaining the morphology of the ER, including ER bodies, and employed a forward-genetic approach using transgenic <em>Arabidopsis thaliana</em> (GFP-h) with fluorescently-labeled ER. We isolated and investigated a mutant (designated <em>endoplasmic reticulum morphology3</em>, <em>ermo3</em>) with huge aggregates and abnormal punctate structures of ER. <em>ERMO3</em> encodes a GDSL-lipase/esterase family protein, also known as MVP1. Here, we showed that, although ERMO3/MVP1/GOLD36 was expressed ubiquitously, the morphological defects of <em>ermo3</em> were specifically seen in a certain type of cells where ER bodies developed. Coimmunoprecipitation analysis combined with mass spectrometry revealed that ERMO3/MVP1/GOLD36 interacts with the PYK10 complex, a huge protein complex that is thought to be important for ER body-related defense systems. We also found that the depletion of transcription factor NAI1, a master regulator for ER body formation, suppressed the formation of ER-aggregates in <em>ermo3</em> cells, suggesting that NAI1 expression plays an important role in the abnormal aggregation of ER. Our results suggest that ERMO3/MVP1/GOLD36 is required for preventing ER and other organelles from abnormal aggregation and for maintaining proper ER morphology in a coordinated manner with NAI1.</p> </div>", "links"=>[], "tags"=>["type-specific", "maintaining", "er", "morphology"], "article_id"=>117483, "categories"=>["Cell Biology", "Genetics"], "users"=>["Ryohei Thomas Nakano", "Ryo Matsushima", "Atsushi J. Nagano", "Yoichiro Fukao", "Masayuki Fujiwara", "Maki Kondo", "Mikio Nishimura", "Ikuko Hara-Nishimura"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0049103.s001", "https://dx.doi.org/10.1371/journal.pone.0049103.s002", "https://dx.doi.org/10.1371/journal.pone.0049103.s003", "https://dx.doi.org/10.1371/journal.pone.0049103.s004", "https://dx.doi.org/10.1371/journal.pone.0049103.s005", "https://dx.doi.org/10.1371/journal.pone.0049103.s006"], "stats"=>{"downloads"=>51, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/ERMO3_MVP1_GOLD36_Is_Involved_in_a_Cell_Type_Specific_Mechanism_for_Maintaining_ER_Morphology_in_Arabidopsis_thaliana__/117483", "title"=>"ERMO3/MVP1/GOLD36 Is Involved in a Cell Type-Specific Mechanism for Maintaining ER Morphology in <em>Arabidopsis thaliana</em>", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-11-14 02:04:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/543345"], "description"=>"<p>Depletion of <i>NAI1</i> in <i>ermo3-1</i> suppressed the formation of ER-aggregates. (<b>A</b>) Single optical sections of cotyledonary epidermal cells of indicated genotypes. Solid and open arrows indicate ER bodies and ER-aggregates, respectively. Bars, 10 µm. (<b>B</b>) 3D reconstructed images of cotyledon and hypocotyl. Bars, 100 µm.</p>", "links"=>[], "tags"=>["was", "cells"], "article_id"=>213837, "categories"=>["Cell Biology", "Genetics", "Plant Biology"], "users"=>["Ryohei Thomas Nakano", "Ryo Matsushima", "Atsushi J. Nagano", "Yoichiro Fukao", "Masayuki Fujiwara", "Maki Kondo", "Mikio Nishimura", "Ikuko Hara-Nishimura"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0049103.g008", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_NAI1_expression_was_required_for_ermo3_1_cells_to_form_ER_aggregates_/213837", "title"=>"NAI1 expression was required for <i>ermo3-1</i> cells to form ER-aggregates.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-11-14 01:03:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/543066"], "description"=>"<p>(<b>A</b>) and (<b>B</b>) Z-projected images (<b>A</b>) or confocal micrographs (<b>B</b>) of rosette leaves from 2-week-old GFP-h and <i>ermo3-2</i> plants. Enlarged views of boxed regions are shown in bottom panels (<b>a</b>) to (<b>d</b>). Arrows, open arrows, and arrowheads indicate ER bodies, ER-aggregate in <i>ermo3</i>, and aberrant punctate structures in <i>ermo3</i>, respectively. Bars, 50 µm (<b>A</b>) and 10 µm (<b>B</b>). (<b>C</b>) Z-projection (upper row; bars, 50 µm) or peripheral section (bottom row; bars, 10 µm) of cotyledon epidermal cells from 2-week-old GFP-h and <i>ermo3-2</i> plants. (<b>D</b>) Summarized phenotypes of <i>ermo3</i> cells during cell development.</p>", "links"=>[], "tags"=>["er", "bodies", "er-aggregates", "correlated"], "article_id"=>213550, "categories"=>["Cell Biology", "Genetics", "Plant Biology"], "users"=>["Ryohei Thomas Nakano", "Ryo Matsushima", "Atsushi J. Nagano", "Yoichiro Fukao", "Masayuki Fujiwara", "Maki Kondo", "Mikio Nishimura", "Ikuko Hara-Nishimura"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0049103.g006", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Development_of_ER_bodies_and_ER_aggregates_were_highly_correlated_in_ermo3_1_/213550", "title"=>"Development of ER bodies and ER-aggregates were highly correlated in <i>ermo3-1</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-11-14 00:59:10"}
  • {"files"=>["https://ndownloader.figshare.com/files/543480"], "description"=>"<p>Cotyledons (<b>A</b>) and rosette leaves (<b>B</b>) from 10-day-old Col-0 and <i>ermo3-2</i> plants expressing ST-GFP were observed under a confocal microscope. In wild type cells, ST-GFP clearly labeled Golgi bodies. In <i>ermo3-2</i> cells, ST-GFP labeled ER network as well as Golgi bodies (insets). This labeling was detected in the cells with aggregates (an open arrow) but not in the cells without obvious aberrant structures (an asterisk). Bars, 10 µm.</p>", "links"=>[], "tags"=>["was", "er-aggregates"], "article_id"=>213970, "categories"=>["Cell Biology", "Genetics", "Plant Biology"], "users"=>["Ryohei Thomas Nakano", "Ryo Matsushima", "Atsushi J. Nagano", "Yoichiro Fukao", "Masayuki Fujiwara", "Maki Kondo", "Mikio Nishimura", "Ikuko Hara-Nishimura"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0049103.g009", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Defective_protein_transport_was_detected_at_the_same_place_with_where_ER_aggregates_were_found_/213970", "title"=>"Defective protein transport was detected at the same place with where ER-aggregates were found.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-11-14 01:06:10"}
  • {"files"=>["https://ndownloader.figshare.com/files/542935"], "description"=>"<p>(<b>A</b>) Seven-day-old seedlings of NT (non-transgenic, identical to Col-0), <i>ermo3</i>-2, and ERMO3-HA were stained with 25 µM FM4-64 for 3 hours. ERMO3-HA did not develop any aggregates, unlike <i>ermo3</i>-2 (open arrows). (<b>B</b>) to (<b>D</b>) Seven-day-old seedlings of NT and ERMO3-HA were subjected to a co-immunoprecipitation (IP) assay using anti-HA antibodies. Total (T), unbound (U), and bound (B) fractions ([<b>B</b>] and [<b>D</b>]), or only the bound fraction (<b>C</b>) from NT and ERMO3-HA, were separated by SDS-PAGE followed by either immunoblot analysis (IB; top and middle panels in [<b>B</b>], and [<b>D</b>]), silver staining (<b>C</b>), or Coomassie Brilliant Blue (CBB) staining (bottom panel in [<b>B</b>]). Numbers shown in (<b>B</b>) and (<b>D</b>) indicate relative amounts of loaded fractions. Interacting proteins identified by mass spectrometry are indicated in (<b>C</b>). GLLs, GDSL lipase-like proteins; JALs, jacalin-related lectins; MATHs, meprin and TRAF homology [MATH] domain-containing proteins.</p>", "links"=>[], "tags"=>["interacts", "pyk10"], "article_id"=>213427, "categories"=>["Cell Biology", "Genetics", "Plant Biology"], "users"=>["Ryohei Thomas Nakano", "Ryo Matsushima", "Atsushi J. Nagano", "Yoichiro Fukao", "Masayuki Fujiwara", "Maki Kondo", "Mikio Nishimura", "Ikuko Hara-Nishimura"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0049103.g005", "stats"=>{"downloads"=>1, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ERMO3_MVP1_GOLD36_interacts_with_PYK10_complex_components_/213427", "title"=>"ERMO3/MVP1/GOLD36 interacts with PYK10 complex components.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-11-14 00:57:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/543611"], "description"=>"‡<p>Proteins with expression levels that are thought to be regulated by NAI1 are indicated by bold characters.</p>*<p>The localizations of these proteins have been experimentally confirmed or suggested <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049103#pone.0049103-Agee1\" target=\"_blank\">[28]</a>, <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049103#pone.0049103-Marti1\" target=\"_blank\">[29]</a>, <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049103#pone.0049103-Matsushima3\" target=\"_blank\">[33]</a>, <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049103#pone.0049103-Nagano2\" target=\"_blank\">[41]</a>.</p>†<p>Correlation coefficients of expression patterns with <i>NAI1</i> (ATTED-II, <a href=\"http://atted.jp/\" target=\"_blank\">http://atted.jp/</a>).</p>", "links"=>[], "tags"=>["interacts", "pyk10"], "article_id"=>214103, "categories"=>["Cell Biology", "Genetics", "Plant Biology"], "users"=>["Ryohei Thomas Nakano", "Ryo Matsushima", "Atsushi J. Nagano", "Yoichiro Fukao", "Masayuki Fujiwara", "Maki Kondo", "Mikio Nishimura", "Ikuko Hara-Nishimura"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0049103.t001", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ERMO3_MVP1_GOLD36_interacts_with_PYK10_complex_components_/214103", "title"=>"ERMO3/MVP1/GOLD36 interacts with PYK10 complex components.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-11-14 01:08:23"}
  • {"files"=>["https://ndownloader.figshare.com/files/542851"], "description"=>"<p>ERMO3/MVP1/GOLD36 protein fused with Venus followed by the ER-retention signal sequence (His-Asp-Glu-Leu) in its C-terminus was stably expressed in Col-0 and in <i>ermo3</i>-2. In both lines, ERMO3-Venus-HDEL localized in the ER lumen and ER bodies. Notably, neither of these developed abnormal aggregates that were seen in <i>ermo3</i> cells.</p>", "links"=>[], "tags"=>["er", "golgi", "bodies", "was", "suppress"], "article_id"=>213337, "categories"=>["Cell Biology", "Genetics", "Plant Biology"], "users"=>["Ryohei Thomas Nakano", "Ryo Matsushima", "Atsushi J. Nagano", "Yoichiro Fukao", "Masayuki Fujiwara", "Maki Kondo", "Mikio Nishimura", "Ikuko Hara-Nishimura"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0049103.g004", "stats"=>{"downloads"=>0, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cycling_between_ER_and_Golgi_bodies_was_sufficient_for_ERMO3_MVP1_GOLD36_to_suppress_ER_aggregation_/213337", "title"=>"Cycling between ER and Golgi bodies was sufficient for ERMO3/MVP1/GOLD36 to suppress ER aggregation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-11-14 00:55:37"}

PMC Usage Stats | Further Information

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Relative Metric

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