Biomarkers of Whale Shark Health: A Metabolomic Approach
Publication Date
November 15, 2012
Journal
PLOS ONE
Authors
Alistair D. M. Dove, Johannes Leisen, Manshui Zhou, Jonathan J. Byrne, et al
Volume
7
Issue
11
Pages
e49379
DOI
https://dx.plos.org/10.1371/journal.pone.0049379
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0049379
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/23166652
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3499553
Europe PMC
http://europepmc.org/abstract/MED/23166652
Web of Science
000311272300040
Scopus
84869216928
Mendeley
http://www.mendeley.com/research/biomarkers-whale-shark-health-metabolomic-approach
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Mendeley | Further Information

{"title"=>"Biomarkers of Whale Shark Health: A Metabolomic Approach", "type"=>"journal", "authors"=>[{"first_name"=>"Alistair D M", "last_name"=>"Dove", "scopus_author_id"=>"7006402899"}, {"first_name"=>"Johannes", "last_name"=>"Leisen", "scopus_author_id"=>"7003994012"}, {"first_name"=>"Manshui", "last_name"=>"Zhou", "scopus_author_id"=>"8307888900"}, {"first_name"=>"Jonathan J.", "last_name"=>"Byrne", "scopus_author_id"=>"44661262600"}, {"first_name"=>"Krista", "last_name"=>"Lim-Hing", "scopus_author_id"=>"36484951000"}, {"first_name"=>"Harry D.", "last_name"=>"Webb", "scopus_author_id"=>"55487128700"}, {"first_name"=>"Leslie", "last_name"=>"Gelbaum", "scopus_author_id"=>"6701382623"}, {"first_name"=>"Mark R.", "last_name"=>"Viant", "scopus_author_id"=>"6701549454"}, {"first_name"=>"Julia", "last_name"=>"Kubanek", "scopus_author_id"=>"6603812071"}, {"first_name"=>"Facundo M.", "last_name"=>"Fernández", "scopus_author_id"=>"7401589166"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"366057664", "sgr"=>"84869216928", "issn"=>"19326203", "pmid"=>"23166652", "scopus"=>"2-s2.0-84869216928", "doi"=>"10.1371/journal.pone.0049379", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)"}, "id"=>"6dc3f41f-c940-309d-acdb-e586e534687a", "abstract"=>"In a search for biomarkers of health in whale sharks and as exploration of metabolomics as a modern tool for understanding animal physiology, the metabolite composition of serum in six whale sharks (Rhincodon typus) from an aquarium collection was explored using (1)H nuclear magnetic resonance (NMR) spectroscopy and direct analysis in real time (DART) mass spectrometry (MS). Principal components analysis (PCA) of spectral data showed that individual animals could be resolved based on the metabolite composition of their serum and that two unhealthy individuals could be discriminated from the remaining healthy animals. The major difference between healthy and unhealthy individuals was the concentration of homarine, here reported for the first time in an elasmobranch, which was present at substantially lower concentrations in unhealthy whale sharks, suggesting that this metabolite may be a useful biomarker of health status in this species. The function(s) of homarine in sharks remain uncertain but it likely plays a significant role as an osmolyte. The presence of trimethylamine oxide (TMAO), another well-known protective osmolyte of elasmobranchs, at 0.1-0.3 mol L(-1) was also confirmed using both NMR and MS. Twenty-three additional potential biomarkers were identified based on significant differences in the frequency of their occurrence between samples from healthy and unhealthy animals, as detected by DART MS. Overall, NMR and MS provided complementary data that showed that metabolomics is a useful approach for biomarker prospecting in poorly studied species like elasmobranchs.", "link"=>"http://www.mendeley.com/research/biomarkers-whale-shark-health-metabolomic-approach", "reader_count"=>71, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>3, "Researcher"=>23, "Student > Ph. D. Student"=>16, "Student > Postgraduate"=>3, "Student > Master"=>7, "Other"=>3, "Student > Bachelor"=>11, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>3, "Researcher"=>23, "Student > Ph. D. Student"=>16, "Student > Postgraduate"=>3, "Student > Master"=>7, "Other"=>3, "Student > Bachelor"=>11, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>7, "Environmental Science"=>11, "Nursing and Health Professions"=>1, "Agricultural and Biological Sciences"=>42, "Medicine and Dentistry"=>4, "Veterinary Science and Veterinary Medicine"=>1, "Chemistry"=>3, "Immunology and Microbiology"=>1, "Earth and Planetary Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Chemistry"=>{"Chemistry"=>3}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Earth and Planetary Sciences"=>{"Earth and Planetary Sciences"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>42}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>1}, "Unspecified"=>{"Unspecified"=>7}, "Environmental Science"=>{"Environmental Science"=>11}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "reader_count_by_country"=>{"Belgium"=>1, "United States"=>1, "Tanzania"=>1, "South Africa"=>1, "Mexico"=>1, "Kenya"=>1}, "group_count"=>4}

Scopus | Further Information

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Figshare

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  • {"files"=>["https://ndownloader.figshare.com/files/541844"], "description"=>"*<p>P<sub>H</sub> and P<sub>M</sub> refer to the proportion of total healthy (n = 16) and unhealthy (n = 37) shark samples, respectively, from which each compound was identified.</p>", "links"=>[], "tags"=>["metabolites", "tentatively", "dart", "ms", "serum"], "article_id"=>212326, "categories"=>["Physiology", "Inorganic Chemistry", "Biochemistry", "Cell Biology"], "users"=>["Alistair D. M. Dove", "Johannes Leisen", "Manshui Zhou", "Jonathan J. Byrne", "Krista Lim-Hing", "Harry D. Webb", "Leslie Gelbaum", "Mark R. Viant", "Julia Kubanek", "Facundo M. Fernández"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0049379.t001", "stats"=>{"downloads"=>7, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Core_candidate_metabolites_detected_and_tentatively_identified_by_DART_MS_from_whale_shark_serum_samples_/212326", "title"=>"“Core” candidate metabolites detected and tentatively identified by DART MS from whale shark serum samples<sup>*</sup>.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-11-15 00:38:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/541467"], "description"=>"<p>: unhealthy individual 1 : unhealthy individual 2 : average for healthy individual 3 (n = 2) : average for healthy individual 4 (n = 3) : average for healthy individual 5 (n = 5).</p>", "links"=>[], "tags"=>["nmr", "spectra", "extracted", "serum", "pc1", "scores", "plotted", "sampling", "unhealthy"], "article_id"=>211945, "categories"=>["Physiology", "Inorganic Chemistry", "Biochemistry", "Cell Biology"], "users"=>["Alistair D. M. Dove", "Johannes Leisen", "Manshui Zhou", "Jonathan J. Byrne", "Krista Lim-Hing", "Harry D. Webb", "Leslie Gelbaum", "Mark R. Viant", "Julia Kubanek", "Facundo M. Fernández"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0049379.g003", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_PCA_of_1_H_NMR_spectra_of_extracted_whale_shark_serum_42_samples_showing_PC1_scores_plotted_against_time_of_sampling_for_the_unhealthy_animals_/211945", "title"=>"PCA of <sup>1</sup>H NMR spectra of extracted whale shark serum (42 samples, showing PC1 scores plotted against time of sampling for the unhealthy animals.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-11-15 00:32:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/541590"], "description"=>"<p>Differences in concentration of homarine (A) and trimethylamine-oxide (TMAO) (B) in serum samples from two unhealthy (animals 1–2) and three healthy (3–5) whale sharks.</p>", "links"=>[], "tags"=>["homarine", "trimethylamine-oxide", "serum", "samples", "unhealthy"], "article_id"=>212074, "categories"=>["Physiology", "Inorganic Chemistry", "Biochemistry", "Cell Biology"], "users"=>["Alistair D. M. Dove", "Johannes Leisen", "Manshui Zhou", "Jonathan J. Byrne", "Krista Lim-Hing", "Harry D. Webb", "Leslie Gelbaum", "Mark R. Viant", "Julia Kubanek", "Facundo M. Fernández"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0049379.g004", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Differences_in_concentration_of_homarine_A_and_trimethylamine_oxide_TMAO_B_in_serum_samples_from_two_unhealthy_animals_1_8211_2_and_three_healthy_3_8211_5_whale_sharks_/212074", "title"=>"Differences in concentration of homarine (A) and trimethylamine-oxide (TMAO) (B) in serum samples from two unhealthy (animals 1–2) and three healthy (3–5) whale sharks.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-11-15 00:34:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/541730"], "description"=>"<p>Loading plot for PC1 as a means to identify NMR spectroscopic features corresponding to relevant metabolites within the serum of whale sharks.</p>", "links"=>[], "tags"=>["pc1", "means", "nmr", "spectroscopic", "corresponding", "metabolites", "serum"], "article_id"=>212209, "categories"=>["Physiology", "Inorganic Chemistry", "Biochemistry", "Cell Biology"], "users"=>["Alistair D. M. Dove", "Johannes Leisen", "Manshui Zhou", "Jonathan J. Byrne", "Krista Lim-Hing", "Harry D. Webb", "Leslie Gelbaum", "Mark R. Viant", "Julia Kubanek", "Facundo M. Fernández"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0049379.g005", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Loading_plot_for_PC1_as_a_means_to_identify_NMR_spectroscopic_features_corresponding_to_relevant_metabolites_within_the_serum_of_whale_sharks_/212209", "title"=>"Loading plot for PC1 as a means to identify NMR spectroscopic features corresponding to relevant metabolites within the serum of whale sharks.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-11-15 00:36:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/541361"], "description"=>"<p>: unhealthy individual 1 : unhealthy individual 2 : healthy individual 3 (n = 2) : healthy individual 4 (n = 3) : healthy individual 5 (n = 5).</p>", "links"=>[], "tags"=>["scores", "plots", "nmr", "ms", "metabolomics", "datasets", "serum", "samples", "analysed"], "article_id"=>211831, "categories"=>["Physiology", "Inorganic Chemistry", "Biochemistry", "Cell Biology"], "users"=>["Alistair D. M. Dove", "Johannes Leisen", "Manshui Zhou", "Jonathan J. Byrne", "Krista Lim-Hing", "Harry D. Webb", "Leslie Gelbaum", "Mark R. Viant", "Julia Kubanek", "Facundo M. Fernández"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0049379.g002", "stats"=>{"downloads"=>1, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_PCA_scores_plots_from_analysis_of_a_NMR_and_b_MS_metabolomics_datasets_of_only_those_whale_shark_serum_samples_that_were_analysed_by_both_methods_/211831", "title"=>"PCA scores plots from analysis of (a) NMR and (b) MS metabolomics datasets of only those whale shark serum samples that were analysed by both methods.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-11-15 00:30:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/541898"], "description"=>"<p>Candidate biomarker metabolites detected and tentatively identified by DART MS that showed a significant difference in frequency between healthy (H) and unhealthy (M) whale sharks (two-proportion z-test <i>p</i><0.05) after Bonferroni correction.</p>", "links"=>[], "tags"=>["biomarker", "metabolites", "tentatively", "dart", "ms", "showed", "unhealthy", "sharks", "z-test", "bonferroni"], "article_id"=>212378, "categories"=>["Physiology", "Inorganic Chemistry", "Biochemistry", "Cell Biology"], "users"=>["Alistair D. M. Dove", "Johannes Leisen", "Manshui Zhou", "Jonathan J. Byrne", "Krista Lim-Hing", "Harry D. Webb", "Leslie Gelbaum", "Mark R. Viant", "Julia Kubanek", "Facundo M. Fernández"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0049379.t002", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Candidate_biomarker_metabolites_detected_and_tentatively_identified_by_DART_MS_that_showed_a_significant_difference_in_frequency_between_healthy_H_and_unhealthy_M_whale_sharks_two_proportion_z_test_p_/212378", "title"=>"Candidate biomarker metabolites detected and tentatively identified by DART MS that showed a significant difference in frequency between healthy (H) and unhealthy (M) whale sharks (two-proportion z-test <i>p</i><0.05) after Bonferroni correction.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-11-15 00:39:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/290068", "https://ndownloader.figshare.com/files/290116"], "description"=>"<div><p>In a search for biomarkers of health in whale sharks and as exploration of metabolomics as a modern tool for understanding animal physiology, the metabolite composition of serum in six whale sharks (<em>Rhincodon typus</em>) from an aquarium collection was explored using <sup>1</sup>H nuclear magnetic resonance (NMR) spectroscopy and direct analysis in real time (DART) mass spectrometry (MS). Principal components analysis (PCA) of spectral data showed that individual animals could be resolved based on the metabolite composition of their serum and that two unhealthy individuals could be discriminated from the remaining healthy animals. The major difference between healthy and unhealthy individuals was the concentration of homarine, here reported for the first time in an elasmobranch, which was present at substantially lower concentrations in unhealthy whale sharks, suggesting that this metabolite may be a useful biomarker of health status in this species. The function(s) of homarine in sharks remain uncertain but it likely plays a significant role as an osmolyte. The presence of trimethylamine oxide (TMAO), another well-known protective osmolyte of elasmobranchs, at 0.1–0.3 mol L<sup>−1</sup> was also confirmed using both NMR and MS. Twenty-three additional potential biomarkers were identified based on significant differences in the frequency of their occurrence between samples from healthy and unhealthy animals, as detected by DART MS. Overall, NMR and MS provided complementary data that showed that metabolomics is a useful approach for biomarker prospecting in poorly studied species like elasmobranchs.</p> </div>", "links"=>[], "tags"=>["biomarkers", "metabolomic", "approach"], "article_id"=>117119, "categories"=>["Physiology", "Inorganic Chemistry", "Biochemistry", "Cell Biology"], "users"=>["Alistair D. M. Dove", "Johannes Leisen", "Manshui Zhou", "Jonathan J. Byrne", "Krista Lim-Hing", "Harry D. Webb", "Leslie Gelbaum", "Mark R. Viant", "Julia Kubanek", "Facundo M. Fernández"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0049379.s001", "https://dx.doi.org/10.1371/journal.pone.0049379.s002"], "stats"=>{"downloads"=>3, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Biomarkers_of_Whale_Shark_Health_A_Metabolomic_Approach__/117119", "title"=>"Biomarkers of Whale Shark Health: A Metabolomic Approach", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-11-15 01:58:39"}

PMC Usage Stats | Further Information

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Relative Metric

{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[316, 541, 663, 766, 856, 950, 1041, 1128, 1218, 1302, 1382, 1456, 1526, 1593, 1657, 1729, 1796, 1862, 1930, 1999, 2065, 2132, 2202, 2261, 2319]}]}
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