Phytochemicals Attenuating Aberrant Activation of β-Catenin in Cancer Cells
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{"title"=>"Phytochemicals Attenuating Aberrant Activation of β-Catenin in Cancer Cells", "type"=>"journal", "authors"=>[{"first_name"=>"Dan", "last_name"=>"Wang", "scopus_author_id"=>"55713384000"}, {"first_name"=>"Mitchell L.", "last_name"=>"Wise", "scopus_author_id"=>"7202820880"}, {"first_name"=>"Feng", "last_name"=>"Li", "scopus_author_id"=>"56402077200"}, {"first_name"=>"Moul", "last_name"=>"Dey", "scopus_author_id"=>"7005967817"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"23226522", "sgr"=>"84870702455", "doi"=>"10.1371/journal.pone.0050508", "scopus"=>"2-s2.0-84870702455", "pui"=>"366216151", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "issn"=>"19326203"}, "id"=>"02361f6d-cab3-335d-824d-cd77f0b14349", "abstract"=>"Phytochemicals are a rich source of chemoprevention agents but their effects on modulating the Wnt/β-catenin signaling pathway have remained largely uninvestigated. Aberrantly activated Wnt signaling can result in the abnormal stabilization of β-catenin, a key causative step in a broad spectrum of cancers. Here we report the modulation of lithium chloride-activated canonical Wnt/β-catenin signaling by phytochemicals that have antioxidant, anti-inflammatory or chemopreventive properties. The compounds were first screened with a cervical cancer-derived stable Wnt signaling reporter HeLa cell line. Positive hits were subsequently evaluated for β-catenin degradation, suppression of β-catenin nuclear localization and down-regulation of downstream oncogenic targets of Wnt/β-catenin pathway. Our study shows a novel degradation path of β-catenin protein in HeLa cells by Avenanthramide 2p (a polyphenol) and Triptolide (a diterpene triepoxide), respectively from oats and a Chinese medicinal plant. The findings present Avenanthramide 2p as a potential chemopreventive dietary compound that merits further study using in vivo models of cancers; they also provide a new perspective on the mechanism of action of Triptolide.", "link"=>"http://www.mendeley.com/research/phytochemicals-attenuating-aberrant-activation-%CE%B2catenin-cancer-cells", "reader_count"=>16, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Researcher"=>2, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>4, "Student > Master"=>1, "Student > Bachelor"=>3, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Researcher"=>2, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>4, "Student > Master"=>1, "Student > Bachelor"=>3, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>9, "Medicine and Dentistry"=>4, "Chemical Engineering"=>1, "Chemistry"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Chemistry"=>{"Chemistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>9}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Chemical Engineering"=>{"Chemical Engineering"=>1}}, "reader_count_by_country"=>{"United States"=>1}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/531065"], "description"=>"<p>A. Lentiviral vector 7TFP contains the 7XTCF promoter, the Firefly luciferase, and the puromycin resistance gene under the control of SV40 promoter. LTR, long terminal repeat; RRE, Rev responsive element; FFluc, Firefly luciferase; SV40, Simian vacuolating virus 40; PuroR, puromycin resistance; WPRE, woodchuck hepatitis virus posttranscriptional regulatory element; SIN; self inactivating. B. Schematic representations of the 7TFP lentiviral production by transfection, lentiviral infection, and stable reporter cell line selection. C. Validation of concentration-dependent Wnt reporter response from the stable producer cell line following LiCl induction (8 h) was measured using Luciferase activity. Folds of Wnt reporter activity relative to negative control (vehicle-treated) are shown as mean±S.E.</p>", "links"=>[], "tags"=>["lentiviral", "induced", "hela", "7tfp", "wnt"], "article_id"=>201546, "categories"=>["Cancer", "Biotechnology", "Chemistry", "Genetics", "Plant Biology"], "users"=>["Dan Wang", "Mitchell L. Wise", "Feng Li", "Moul Dey"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0050508.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Production_of_lentiviral_induced_stable_Hela_7TFP_Wnt_reporter_cell_line_/201546", "title"=>"Production of lentiviral induced stable Hela 7TFP Wnt reporter cell line.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 19:13:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/531179"], "description"=>"<p>Reporter and Renilla luciferase activities were measured using a Dual Glo luciferase assay kit. Normalized percentage values relative to LiCl control are shown as the mean±S.E (n = 4). Asterisks indicate statistically significant differences between compound<i>-</i>treated cells and LiCl-induced control: ***p≤0.001, **p≤0.01, * p≤0.05.</p>", "links"=>[], "tags"=>["wnt", "transcription", "phytochemicals", "hela", "7tfp"], "article_id"=>201668, "categories"=>["Cancer", "Biotechnology", "Chemistry", "Genetics", "Plant Biology"], "users"=>["Dan Wang", "Mitchell L. Wise", "Feng Li", "Moul Dey"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0050508.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Attenuation_of_Wnt_transcription_by_phytochemicals_in_HeLa_7TFP_reporter_cells_/201668", "title"=>"Attenuation of Wnt transcription by phytochemicals in HeLa 7TFP reporter cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 19:13:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/531321"], "description"=>"<p>Hela cells were cultured with MTS culture medium. Absorbance was read at 490 nm and data were expressed as percent cell viability compared with DMSO control (vehicle). Normalized percentage values relative to DMSO control are shown as the mean±S.E (n = 4). Asterisks indicate statistically significant differences between compound<i>-</i>treated cells and control: ***p≤0.001, **p≤0.01, * p≤0.05.</p>", "links"=>[], "tags"=>["2p", "triptolide", "defer", "hela"], "article_id"=>201813, "categories"=>["Cancer", "Biotechnology", "Chemistry", "Genetics", "Plant Biology"], "users"=>["Dan Wang", "Mitchell L. Wise", "Feng Li", "Moul Dey"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0050508.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Avn_2p_and_Triptolide_defer_HeLa_cell_proliferation_/201813", "title"=>"Avn 2p and Triptolide defer HeLa cell proliferation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 19:14:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/531490"], "description"=>"<p>A. β-catenin protein expression: Western blot was double probed with mouse anti human β-catenin (1∶2000 v/v) and rabbit anti human β-actin (1∶5000 v/v) antibodies, washed in phosphate buffered saline, and further incubated with Dylight 680 anti-mouse (1∶5000 v/v) and Dylight 800 anti-rabbit (1∶5000 v/v) antibodies. LI-COR odyssey Infrared Imaging System was used in immunoblotting signal detection. B. Densitometric analysis of β-catenin protein levels (mean±SE, n = 3): β-actin was used as housekeeping control for normalization. Asterisk indicates expression levels of β-catenin in compounds-treated cells that are significantly different from that of LiCl induced control: ***p≤0.001, **p≤0.01, * p≤0.05.</p>", "links"=>[], "tags"=>["2p", "triptolide", "induced", "cellular", "degradation"], "article_id"=>201980, "categories"=>["Cancer", "Biotechnology", "Chemistry", "Genetics", "Plant Biology"], "users"=>["Dan Wang", "Mitchell L. Wise", "Feng Li", "Moul Dey"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0050508.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Avn_2p_and_Triptolide_induced_cellular_degradation_of_946_catenin_/201980", "title"=>"Avn 2p and Triptolide induced cellular degradation of β-catenin.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 19:15:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/531679"], "description"=>"<p>A. Microscopic detection of β-catenin localization by immunofluorescence: HeLa cells were treated with vehicle only (DMSO, top panel), 15 µM FH535 (second from top), 80 µM Avn 2p (third from top) or 22 nM Triptolide (bottom) prior to 50 mM LiCl induction for each. Cells were immunostained with mouse anti β-catenin (1∶1000 v/v) and goat anti mouse Dylight 488 (1∶2000 v/v) antibodies (green), and Hoechst (0.1 µg/ml, blue). Yellow arrows indicate the presence of β-catenin in the nucleus whereas white arrows point to the absence of β-catenin in the nucleus. B. Statistical analysis of β-catenin nucleus localization. Nucleus localization of β-catenin was determined by Dylight 488 staining in the nucleus. The cells with presence of β-catenin in the nucleus were counted among 100 cells for each treatment. Number of cells exhibiting the nuclear translocation of β-catenin is shown in dark green, while number of cells not showing β-catenin’s nuclear localization is shown in white. Asterisks indicate statistically significant differences between compound<i>-</i>treated cells and control: ***p≤0.001, **p≤0.01, * p≤0.05, n = 3.</p>", "links"=>[], "tags"=>["2p", "triptolide", "reduced", "nucleus", "abundance", "hela"], "article_id"=>202165, "categories"=>["Cancer", "Biotechnology", "Chemistry", "Genetics", "Plant Biology"], "users"=>["Dan Wang", "Mitchell L. Wise", "Feng Li", "Moul Dey"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0050508.g005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Avn_2p_and_Triptolide_reduced_nucleus_abundance_of_946_catenin_in_HeLa_cells_/202165", "title"=>"Avn 2p and Triptolide reduced nucleus abundance of β-catenin in HeLa cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 19:16:24"}
  • {"files"=>["https://ndownloader.figshare.com/files/531818"], "description"=>"<p>The concentration-dependent effects of treatments on c-Myc gene expression was measured by the mRNA quantity relative to the response to LiCl activation only (positive control) that is normalized to a value of 1.00; lower values represent greater inhibitory effects with 0.00 corresponding to a complete inhibition of the induced gene expression. Total RNA was extracted, purified and cDNA was synthesized. Relative quantification using SYBR green technology and standard ΔΔCt method was used for individual RT-PCR. Relative values are mean±S.E (n = 3). Asterisks indicate statistically significant differences between compound<i>-</i> treated cells and control: ***p≤0.001, **p≤0.01, * p≤0.05.</p>", "links"=>[], "tags"=>["2p", "triptolide", "inhibit", "c-myc", "transcription", "licl-elicited", "hela"], "article_id"=>202304, "categories"=>["Cancer", "Biotechnology", "Chemistry", "Genetics", "Plant Biology"], "users"=>["Dan Wang", "Mitchell L. Wise", "Feng Li", "Moul Dey"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0050508.g006"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Avn_2p_and_Triptolide_inhibit_c_Myc_transcription_in_LiCl_elicited_HeLa_cells_/202304", "title"=>"Avn 2p and Triptolide inhibit c-Myc transcription in LiCl-elicited HeLa cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 19:17:09"}

PMC Usage Stats | Further Information

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Relative Metric

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