Immobilization of Pseudorabies Virus in Porcine Tracheal Respiratory Mucus Revealed by Single Particle Tracking
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{"title"=>"Immobilization of Pseudorabies Virus in Porcine Tracheal Respiratory Mucus Revealed by Single Particle Tracking", "type"=>"journal", "authors"=>[{"first_name"=>"Xiaoyun", "last_name"=>"Yang", "scopus_author_id"=>"54793753100"}, {"first_name"=>"Katrien", "last_name"=>"Forier", "scopus_author_id"=>"36918055700"}, {"first_name"=>"Lennert", "last_name"=>"Steukers", "scopus_author_id"=>"36239413600"}, {"first_name"=>"Sandra", "last_name"=>"van Vlierberghe", "scopus_author_id"=>"16023577900"}, {"first_name"=>"Peter", "last_name"=>"Dubruel", "scopus_author_id"=>"6602421164"}, {"first_name"=>"Kevin", "last_name"=>"Braeckmans", "scopus_author_id"=>"6506414516"}, {"first_name"=>"Sarah", "last_name"=>"Glorieux", "scopus_author_id"=>"16202591500"}, {"first_name"=>"Hans J.", "last_name"=>"Nauwynck", "scopus_author_id"=>"7007141390"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84870912120", "doi"=>"10.1371/journal.pone.0051054", "pui"=>"366250485", "pmid"=>"23236432", "scopus"=>"2-s2.0-84870912120", "issn"=>"19326203", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)"}, "id"=>"53d54a50-a6ce-3d2f-8f8d-ed4cda2b27cc", "abstract"=>"Pseudorabies virus (PRV) initially replicates in the porcine upper respiratory tract. It easily invades the mucosae and submucosae for subsequent spread throughout the body via blood vessels and nervous system. In this context, PRV developed ingenious processes to overcome different barriers such as epithelial cells and the basement membrane. Another important but often overlooked barrier is the substantial mucus layer which coats the mucosae. However, little is known about how PRV particles interact with porcine respiratory mucus. We therefore measured the barrier properties of porcine tracheal respiratory mucus, and investigated the mobility of nanoparticles including PRV in this mucus. We developed an in vitro model utilizing single particle tracking microscopy. Firstly, the mucus pore size was evaluated with polyethylene glycol coupled (PEGylated) nanoparticles and atomic force microscope. Secondly, the mobility of PRV in porcine tracheal respiratory mucus was examined and compared with that of negative, positive and PEGylated nanoparticles. The pore size of porcine tracheal respiratory mucus ranged from 80 to 1500 nm, with an average diameter of 455+/-240 nm. PRV (zeta potential: -31.8+/-1.5 mV) experienced a severe obstruction in porcine tracheal respiratory mucus, diffusing 59-fold more slowly than in water. Similarly, the highly negatively (-49.8+/-0.6 mV) and positively (36.7+/-1.1 mV) charged nanoparticles were significantly trapped. In contrast, the nearly neutral, hydrophilic PEGylated nanoparticles (-9.6+/-0.8 mV) diffused rapidly, with the majority of particles moving 50-fold faster than PRV. The mobility of the particles measured was found to be related but not correlated to their surface charge. Furthermore, PEGylated PRV (-13.8+/-0.9 mV) was observed to diffuse 13-fold faster than native PRV. These findings clearly show that the mobility of PRV was significantly hindered in porcine tracheal respiratory mucus, and that the obstruction of PRV was due to complex mucoadhesive interactions including charge interactions rather than size exclusion.", "link"=>"http://www.mendeley.com/research/immobilization-pseudorabies-virus-porcine-tracheal-respiratory-mucus-revealed-single-particle-tracki", "reader_count"=>35, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Researcher"=>7, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>15, "Student > Master"=>7, "Other"=>4}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Researcher"=>7, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>15, "Student > Master"=>7, "Other"=>4}, "reader_count_by_subject_area"=>{"Engineering"=>3, "Unspecified"=>2, "Environmental Science"=>1, "Materials Science"=>4, "Mathematics"=>3, "Agricultural and Biological Sciences"=>10, "Medicine and Dentistry"=>3, "Pharmacology, Toxicology and Pharmaceutical Science"=>3, "Physics and Astronomy"=>1, "Chemistry"=>4, "Social Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>3}, "Materials Science"=>{"Materials Science"=>4}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Chemistry"=>{"Chemistry"=>4}, "Social Sciences"=>{"Social Sciences"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>10}, "Mathematics"=>{"Mathematics"=>3}, "Unspecified"=>{"Unspecified"=>2}, "Environmental Science"=>{"Environmental Science"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>3}}, "reader_count_by_country"=>{"Greece"=>1, "United States"=>1}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/528783"], "description"=>"<p>(A) AFM visualization (5 µm×5 µm) of porcine tracheal respiratory mucus. (B) Pore size distribution of the porcine tracheal respiratory mucus as measured by AFM. Pore diameters were analyzed by the line profiling of the 3-D images. Three scans were performed for each sample, and three independent samples were measured. The dashed line indicates the average of pore diameters (n = 500). (C) Transport modes of 100, 200 and 500 nm PEGylated nanoparticles indicated by <i>α</i> value. More than 2000 trajectories from 3 independent experiments were tested to obtain <i>α</i>. (D) Distributions of the apparent diffusion coefficient of PEGylated nanoparticles. Trajectories of 10 steps were analyzed for each of the 2000 diffusion coefficients. Distributions were refined with MEM.</p>", "links"=>[], "tags"=>["microstructure", "porcine", "tracheal", "respiratory"], "article_id"=>199262, "categories"=>["Virology", "Biotechnology"], "users"=>["Xiaoyun Yang", "Katrien Forier", "Lennert Steukers", "Sandra Van Vlierberghe", "Peter Dubruel", "Kevin Braeckmans", "Sarah Glorieux", "Hans J. Nauwynck"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0051054.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Evaluation_of_the_microstructure_of_porcine_tracheal_respiratory_mucus_/199262", "title"=>"Evaluation of the microstructure of porcine tracheal respiratory mucus.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 19:00:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/528884"], "description"=>"<p>(A) Averaged ensemble mean squared displacements <<i>MSD</i>> of PRV and 200 nm PEGylated nanoparticles with respect to time scale. Error bars indicate standard errors of the mean. (B) Distributions of the apparent diffusion coefficient of PRV and 200 nm PEGylated nanoparticles. Trajectories of 10 steps were analyzed for each of the 2000 diffusion coefficients. Distributions were refined with MEM. (C) Ratios of the average diffusion coefficients in mucus (<i>D<sub>m</sub></i>) to those in water (<i>D<sub>w</sub></i>). Three independent experiments were performed for each condition. Error bars indicate the standard deviation. The asterisk (*) indicates statistical significance (P<0.005).</p>", "links"=>[], "tags"=>["rates", "prv", "200", "nm", "pegylated", "nanoparticles", "porcine", "tracheal", "respiratory", "mucus"], "article_id"=>199366, "categories"=>["Virology", "Biotechnology"], "users"=>["Xiaoyun Yang", "Katrien Forier", "Lennert Steukers", "Sandra Van Vlierberghe", "Peter Dubruel", "Kevin Braeckmans", "Sarah Glorieux", "Hans J. Nauwynck"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0051054.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Transport_rates_of_PRV_and_200_nm_PEGylated_nanoparticles_in_porcine_tracheal_respiratory_mucus_or_in_water_/199366", "title"=>"Transport rates of PRV and 200 nm PEGylated nanoparticles in porcine tracheal respiratory mucus or in water.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 19:01:22"}
  • {"files"=>["https://ndownloader.figshare.com/files/528973"], "description"=>"<p>(A) ζ potentials for the particles measured with laser Doppler anemometry. (B) Representative trajectories of PRV and 200 nm nanoparticles with different surface charge modifications. The diffusions of negatively and positively charged nanoparticles and PRV were significantly suppressed; the PEGylated nanoparticles diffused rapidly. (C) Diffusion coefficient of the PRV and modified nanoparticles. Apparent diffusion coefficients <i>D<sub>a</sub></i> were determined by particle tracking for ensembles of 2000 particles for each condition. Error bars indicate the standard error of the mean.</p>", "links"=>[], "tags"=>["Virology", "biotechnology"], "article_id"=>199469, "categories"=>["Virology", "Biotechnology"], "users"=>["Xiaoyun Yang", "Katrien Forier", "Lennert Steukers", "Sandra Van Vlierberghe", "Peter Dubruel", "Kevin Braeckmans", "Sarah Glorieux", "Hans J. Nauwynck"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0051054.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Particle_surface_charge_and_particle_mobility_/199469", "title"=>"Particle surface charge and particle mobility.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 19:01:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/529129"], "description"=>"<p>Schematic structure of mucin glycoproteins and their potentially mucoadhesive elements.</p>", "links"=>[], "tags"=>["mucin", "glycoproteins", "mucoadhesive"], "article_id"=>199619, "categories"=>["Virology", "Biotechnology"], "users"=>["Xiaoyun Yang", "Katrien Forier", "Lennert Steukers", "Sandra Van Vlierberghe", "Peter Dubruel", "Kevin Braeckmans", "Sarah Glorieux", "Hans J. Nauwynck"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0051054.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Schematic_structure_of_mucin_glycoproteins_and_their_potentially_mucoadhesive_elements_/199619", "title"=>"Schematic structure of mucin glycoproteins and their potentially mucoadhesive elements.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 19:02:42"}
  • {"files"=>["https://ndownloader.figshare.com/files/529236"], "description"=>"<p>Diffusion comparison of PRV and PEGylated PRV.</p>", "links"=>[], "tags"=>["prv", "pegylated"], "article_id"=>199733, "categories"=>["Virology", "Biotechnology"], "users"=>["Xiaoyun Yang", "Katrien Forier", "Lennert Steukers", "Sandra Van Vlierberghe", "Peter Dubruel", "Kevin Braeckmans", "Sarah Glorieux", "Hans J. Nauwynck"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0051054.t002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Diffusion_comparison_of_PRV_and_PEGylated_PRV_/199733", "title"=>"Diffusion comparison of PRV and PEGylated PRV.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-19 19:03:16"}
  • {"files"=>["https://ndownloader.figshare.com/files/529275"], "description"=>"*<p>Provided by the manufacture.</p>", "links"=>[], "tags"=>["pegylated"], "article_id"=>199769, "categories"=>["Virology", "Biotechnology"], "users"=>["Xiaoyun Yang", "Katrien Forier", "Lennert Steukers", "Sandra Van Vlierberghe", "Peter Dubruel", "Kevin Braeckmans", "Sarah Glorieux", "Hans J. Nauwynck"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0051054.t001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Surface_charge_and_size_of_the_PEGylated_nanoparticles_/199769", "title"=>"Surface charge and size of the PEGylated nanoparticles.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-19 19:03:28"}

PMC Usage Stats | Further Information

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Relative Metric

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