Protection of the Photosynthetic Apparatus from Extreme Dehydration and Oxidative Stress in Seedlings of Transgenic Tobacco
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{"title"=>"Protection of the Photosynthetic Apparatus from Extreme Dehydration and Oxidative Stress in Seedlings of Transgenic Tobacco", "type"=>"journal", "authors"=>[{"first_name"=>"Concepción", "last_name"=>"Almoguera", "scopus_author_id"=>"6701834605"}, {"first_name"=>"Pilar", "last_name"=>"Prieto-Dapena", "scopus_author_id"=>"6508122446"}, {"first_name"=>"José María", "last_name"=>"Personat", "scopus_author_id"=>"55516404000"}, {"first_name"=>"Javier", "last_name"=>"Tejedor-Cano", "scopus_author_id"=>"36545532000"}, {"first_name"=>"Marika", "last_name"=>"Lindahl", "scopus_author_id"=>"7005729390"}, {"first_name"=>"Antonio", "last_name"=>"Diaz-Espejo", "scopus_author_id"=>"14031048300"}, {"first_name"=>"Juan", "last_name"=>"Jordano", "scopus_author_id"=>"7003919946"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pmid"=>"23227265", "doi"=>"10.1371/journal.pone.0051443", "pui"=>"366234397", "issn"=>"19326203", "sgr"=>"84870854053", "scopus"=>"2-s2.0-84870854053"}, "id"=>"bb09b2b7-32aa-369d-8b86-cad7dceeb4c9", "abstract"=>"A genetic program that in sunflower seeds is activated by Heat Shock transcription Factor A9 (HaHSFA9) has been analyzed in transgenic tobacco seedlings. The ectopic overexpression of the HSFA9 program protected photosynthetic membranes, which resisted extreme dehydration and oxidative stress conditions. In contrast, heat acclimation of seedlings induced thermotolerance but not resistance to the harsh stress conditions employed. The HSFA9 program was found to include the expression of plastidial small Heat Shock Proteins that accumulate only at lower abundance in heat-stressed vegetative organs. Photosystem II (PSII) maximum quantum yield was higher for transgenic seedlings than for non-transgenic seedlings, after either stress treatment. Furthermore, protection of both PSII and Photosystem I (PSI) membrane protein complexes was observed in the transgenic seedlings, leading to their survival after the stress treatments. It was also shown that the plastidial D1 protein, a labile component of the PSII reaction center, and the PSI core protein PsaB were shielded from oxidative damage and degradation. We infer that natural expression of the HSFA9 program during embryogenesis may protect seed pro-plastids from developmental desiccation.", "link"=>"http://www.mendeley.com/research/protection-photosynthetic-apparatus-extreme-dehydration-oxidative-stress-seedlings-transgenic-tobacc", "reader_count"=>18, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>4, "Student > Doctoral Student"=>1, "Researcher"=>5, "Student > Ph. D. Student"=>1, "Student > Postgraduate"=>1, "Student > Master"=>1, "Other"=>1, "Student > Bachelor"=>1, "Professor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>4, "Student > Doctoral Student"=>1, "Researcher"=>5, "Student > Ph. D. Student"=>1, "Student > Postgraduate"=>1, "Student > Master"=>1, "Other"=>1, "Student > Bachelor"=>1, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>12, "Chemistry"=>1}, "reader_count_by_subdiscipline"=>{"Chemistry"=>{"Chemistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>12}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>3}}, "reader_count_by_country"=>{"Peru"=>1}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/531632"], "description"=>"<p>(<b>A</b>) Maximum quantum yield (F<sub>v</sub>/F<sub>m</sub>) of PSII for 35S:A9 seedlings (T) compared to sibling NT seedlings. The effects of standard H<sub>2</sub>O<sub>2</sub> treatments were analyzed as in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051443#pone-0051443-g003\" target=\"_blank\">Figure 3B</a> (compare the C and H<sub>2</sub>O<sub>2</sub> samples), and using seedlings that were first heat-acclimated during 3 h at 40°C. The corresponding control and H<sub>2</sub>O<sub>2</sub>-treated samples are labeled 40°C and H<sub>2</sub>O<sub>2</sub> (40°C), respectively. The average values for three to four experiments performed with three different T/NT line pairs are shown. Numbers in brackets indicate the different F<sub>v</sub>/F<sub>m</sub> determinations performed in each case. Heat-acclimation did not induce significant differences in F<sub>v</sub>/F<sub>m</sub> after H<sub>2</sub>O<sub>2</sub> treatments in NT (F = 1.94, P = 0.16) or T (F = 3.32, P = 0.07) seedlings. (<b>B</b>) The heat-acclimated NT seedlings did not survive the H<sub>2</sub>O<sub>2</sub> treatments. (<b>C</b>) Survival of non-acclimated T seedlings. (<b>D</b>) Survival of heat-acclimated T seedlings.</p>", "links"=>[], "tags"=>["acclimation", "psii", "caused", "drastic", "oxidative"], "article_id"=>202116, "categories"=>["Biotechnology", "Cell Biology", "Genetics", "Plant Biology"], "users"=>["Concepción Almoguera", "Pilar Prieto-Dapena", "José-María Personat", "Javier Tejedor-Cano", "Marika Lindahl", "Antonio Diaz-Espejo", "Juan Jordano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0051443.g007", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Heat_acclimation_does_not_protect_PSII_from_damage_caused_by_the_drastic_oxidative_stress_conditions_/202116", "title"=>"Heat acclimation does not protect PSII from damage caused by the drastic oxidative stress conditions.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-12-05 00:35:16"}
  • {"files"=>["https://ndownloader.figshare.com/files/531119"], "description"=>"<p>(<b>A</b>) The 35S:A9 seedlings survive drastic oxidative stress conditions. Representative results for the T<sub>2</sub>/NT<sub>2</sub> line pair are shown. Differences in green color were evident between seedlings grown in control conditions (C). Most 35S:A9 seedlings (T<sub>2</sub>) survived treatments for 24 h with 200 mM H<sub>2</sub>O<sub>2</sub> (H<sub>2</sub>O<sub>2</sub>) that caused complete death of sibling NT<sub>2</sub> seedlings. The 3x-magnified inset shows bleaching of the cotyledons in the surviving T<sub>2</sub> seedlings. Scale bar, 1 cm. (<b>B</b>) Comparison of maximum quantum yield (F<sub>v</sub>/F<sub>m</sub>) of PSII between 35S:A9 (T) and NT seedlings after control (C) and H<sub>2</sub>O<sub>2</sub> treatments (H<sub>2</sub>O<sub>2</sub>). Average values for nine independent experiments performed with three different T/NT line pairs. Statistical difference between the F<sub>v</sub>/F<sub>m</sub> values (F = 265.58, P = 0.0001) and rest of symbols indicated as in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051443#pone-0051443-g002\" target=\"_blank\">Figure 2A</a>. (<b>C</b>) PSII membrane protein complexes of 35S:A9 seedlings partially resisted the H<sub>2</sub>O<sub>2</sub> treatments. Thylakoids from control (C) and H<sub>2</sub>O<sub>2</sub> treated seedlings (H<sub>2</sub>O<sub>2</sub>) were analyzed essentially as indicated in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051443#pone-0051443-g002\" target=\"_blank\">Figure 2B</a>. The H<sub>2</sub>O<sub>2</sub> samples were prepared immediately after the treatment and the gel was photographed after BN-PAGE without further staining. Symbols for PSII complexes as in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051443#pone-0051443-g002\" target=\"_blank\">Figure 2B</a>.</p>", "links"=>[], "tags"=>["psii", "caused", "drastic", "oxidative"], "article_id"=>201610, "categories"=>["Biotechnology", "Cell Biology", "Genetics", "Plant Biology"], "users"=>["Concepción Almoguera", "Pilar Prieto-Dapena", "José-María Personat", "Javier Tejedor-Cano", "Marika Lindahl", "Antonio Diaz-Espejo", "Juan Jordano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0051443.g003", "stats"=>{"downloads"=>0, "page_views"=>39, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Protection_of_PSII_from_damage_caused_by_drastic_oxidative_stress_conditions_/201610", "title"=>"Protection of PSII from damage caused by drastic oxidative stress conditions.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-12-05 00:26:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/531475"], "description"=>"<p>(<b>A</b>) Accumulation of the D1 protein under control growth conditions (C) and after treatments with H<sub>2</sub>O<sub>2</sub> (H<sub>2</sub>O<sub>2</sub>). In each case the seedlings were exposed to 6 h normal light conditions in absence or in presence of 1mM lincomycin (L). Sample labels and rest of symbols as in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051443#pone-0051443-g005\" target=\"_blank\">Figure 5A</a>. Protein amounts loaded per lane as in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051443#pone-0051443-g005\" target=\"_blank\">Figure 5</a>. (<b>B</b>) Comparison of maximum quantum yield (F<sub>v</sub>/F<sub>m</sub>) of PSII between 35S:A9 seedlings (T) and sibling NT seedlings in the experimental conditions used in (A). We show average values from a representative experiment performed with three different T/NT line pairs (n = 18, for all conditions). Asterisks indicate the significant statistical differences found between the F<sub>v</sub>/F<sub>m</sub> values obtained in absence and presence of lincomycin: for control NT seedlings (F = 111.6, P = 0.0001); for control T seedlings, (F = 59.21, P = 0.0001); and for stressed T seedlings (F = 37.75, P = 0.0001).</p>", "links"=>[], "tags"=>["degradation", "d1", "synthesis"], "article_id"=>201966, "categories"=>["Biotechnology", "Cell Biology", "Genetics", "Plant Biology"], "users"=>["Concepción Almoguera", "Pilar Prieto-Dapena", "José-María Personat", "Javier Tejedor-Cano", "Marika Lindahl", "Antonio Diaz-Espejo", "Juan Jordano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0051443.g006", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Delayed_degradation_of_the_D1_protein_and_protection_of_the_D1_protein_synthesis_in_the_35S_A9_seedlings_/201966", "title"=>"Delayed degradation of the D1 protein and protection of the D1 protein synthesis in the 35S:A9 seedlings.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-12-05 00:32:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/530996"], "description"=>"<p>(<b>A</b>) Comparison of maximum quantum yield (F<sub>v</sub>/F<sub>m</sub>) values of transgenic seedlings (T) with those for the non-transgenic seedlings (NT), determined in control growth conditions (C) and immediately after the dehydration treatment [DT (0 h)]. Average values obtained in 4 to 5 independent experiments performed with two different T/NT line pairs. The difference observed for the DT (0 h) values was statistically significant (F = 194.41, P = 0.0001) as indicated by the asterisk. Numbers in brackets indicate the different F<sub>v</sub>/F<sub>m</sub> determinations performed in each case. (<b>B</b>) Enhanced stability of PSII membrane protein complexes. The complexes were visualized by BN-PAGE using samples prepared from dehydrated seedlings analyzed immediately after the dehydration treatment (0 h), and following rehydration for 1 h (1 h). The gel was stained with colloidal Coomassie blue. Symbols (•, and the bracket on top) mark the dimeric PSII complex and the PSII super-complexes mentioned in the text. Representative results for the T<sub>1</sub>/NT<sub>1</sub> sibling pair are shown.</p>", "links"=>[], "tags"=>["psii", "caused"], "article_id"=>201490, "categories"=>["Biotechnology", "Cell Biology", "Genetics", "Plant Biology"], "users"=>["Concepción Almoguera", "Pilar Prieto-Dapena", "José-María Personat", "Javier Tejedor-Cano", "Marika Lindahl", "Antonio Diaz-Espejo", "Juan Jordano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0051443.g002", "stats"=>{"downloads"=>0, "page_views"=>32, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Protection_of_PSII_from_damage_caused_by_severe_dehydration_/201490", "title"=>"Protection of PSII from damage caused by severe dehydration.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-12-05 00:24:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/530851"], "description"=>"<p>Immunoblot detection with the anti-HSP21 antibody at 1/2,500 dilution. Different transgenic lines (T) were compared with sibling non-transgenic (NT) lines. From top to bottom: DS10:A9 seeds, DS10:A9M3 seeds, and 35S:A9 seedlings. Protein amounts loaded: 25 μg total protein for the seed samples and for the 40°C seedling sample; 15 μg for the rest of seedling samples. Enhanced detection conditions were used for the DS10:A9M3 samples with respect to DS10:A9 (90 min instead of 5 min exposure, compare the respective NT signals). The high-level accumulation of plastidial sHSPs in unstressed seedlings of the 35S:A9 lines was compared with that in NT seedlings after heat-acclimation for 3 h at 40°C (40°C). Molecular mass markers (kD) are indicated to the left.</p>", "links"=>[], "tags"=>["hahsfa9", "accumulation", "plastidial", "shsps", "seeds"], "article_id"=>201335, "categories"=>["Biotechnology", "Cell Biology", "Genetics", "Plant Biology"], "users"=>["Concepción Almoguera", "Pilar Prieto-Dapena", "José-María Personat", "Javier Tejedor-Cano", "Marika Lindahl", "Antonio Diaz-Espejo", "Juan Jordano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0051443.g001", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_gain_and_loss_of_function_of_HaHSFA9_on_the_accumulation_of_plastidial_sHSPs_in_seeds_and_seedlings_/201335", "title"=>"Effects of gain and loss of function of HaHSFA9 on the accumulation of plastidial sHSPs in seeds and seedlings.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-12-05 00:22:15"}
  • {"files"=>["https://ndownloader.figshare.com/files/531232"], "description"=>"<p>(<b>A</b>) Western detection of complexes after the H<sub>2</sub>O<sub>2</sub> treatments. The PSII complexes (top) separated by BN-PAGE were detected using anti-D1 (DE-loop) antibodies at 1/5,000 dilution. The PSI complexes (bottom) were detected using anti-PsaB antibodies at 1/4,000 dilution. PSII symbols: •, and the bracket on top respectively mark the dimeric PSII complex, and the PSII-LHCII super-complexes. The asterisk marks the CVII (CP43-less PSII monomer) complex. PSI symbols: • marks the PSI-LHCI super-complex that co-migrates in our gel system with the dimeric PSII complex; the asterisk marks the PSI monomer. (<b>B</b>) The PSII complexes also withstand drastic dehydration. The thylakoid samples were analyzed immediately (0 h) after the dehydration treatment (DT), and following rehydration for 16 h, DT (16 h). In this case the complexes were detected using anti-D1 (C-terminal) antibodies at 1/15,000 dilution. An additional PSII complex mentioned in the text is indicated: CV (**, PSII monomer).</p>", "links"=>[], "tags"=>["complexes", "psii", "psi"], "article_id"=>201722, "categories"=>["Biotechnology", "Cell Biology", "Genetics", "Plant Biology"], "users"=>["Concepción Almoguera", "Pilar Prieto-Dapena", "José-María Personat", "Javier Tejedor-Cano", "Marika Lindahl", "Antonio Diaz-Espejo", "Juan Jordano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0051443.g004", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Membrane_protein_complexes_of_PSII_and_PSI_survive_in_the_35S_A9_seedlings_/201722", "title"=>"Membrane protein complexes of PSII and PSI survive in the 35S:A9 seedlings.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-12-05 00:28:42"}
  • {"files"=>["https://ndownloader.figshare.com/files/531324"], "description"=>"<p>(<b>A</b>) Damage by the H<sub>2</sub>O<sub>2</sub> treatments (H<sub>2</sub>O<sub>2</sub>) compared to control conditions (C). (<b>B</b>) Protection from damage by the dehydration treatments (DT). The DT samples were analyzed immediately after the dehydration treatment (0 h), and following rehydration for 16 h (16 h). Thylakoid protein samples from the indicated transgenic lines (T) were compared with sibling non-transgenic (NT) lines. Protein amounts loaded per lane in order to obtain similar initial amounts of the D1 protein: NT<sub>1</sub> (40 μg), T<sub>1</sub> (10 μg), NT<sub>2</sub> (20 μg), T<sub>2</sub> (10 μg), NT<sub>3</sub> (20 μg), and T<sub>3</sub> (10 μg). Immunoblot detection was performed with anti-D1 (DE-loop) antibodies at 1/2,000 (A) or 1/6,000 dilution (B). To the right of each panel, a thin line marks the position of the major D1 band; slightly above, a shaded square marks the retarded mobility D1 band observed after the stress treatments. Asterisks above and below the major D1 bands respectively indicate the cross-linked and degraded D1 protein bands mentioned in the text. Molecular mass markers (kD) are indicated to the left.</p>", "links"=>[], "tags"=>["d1", "psii", "protected", "caused", "oxidative"], "article_id"=>201818, "categories"=>["Biotechnology", "Cell Biology", "Genetics", "Plant Biology"], "users"=>["Concepción Almoguera", "Pilar Prieto-Dapena", "José-María Personat", "Javier Tejedor-Cano", "Marika Lindahl", "Antonio Diaz-Espejo", "Juan Jordano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0051443.g005", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_D1_protein_of_PSII_is_protected_from_damage_caused_by_oxidative_stress_and_severe_dehydration_/201818", "title"=>"The D1 protein of PSII is protected from damage caused by oxidative stress and severe dehydration.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-12-05 00:30:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/286037", "https://ndownloader.figshare.com/files/286086", "https://ndownloader.figshare.com/files/286129", "https://ndownloader.figshare.com/files/286171"], "description"=>"<div><p>A genetic program that in sunflower seeds is activated by Heat Shock transcription Factor A9 (HaHSFA9) has been analyzed in transgenic tobacco seedlings. The ectopic overexpression of the HSFA9 program protected photosynthetic membranes, which resisted extreme dehydration and oxidative stress conditions. In contrast, heat acclimation of seedlings induced thermotolerance but not resistance to the harsh stress conditions employed. The HSFA9 program was found to include the expression of plastidial small Heat Shock Proteins that accumulate only at lower abundance in heat-stressed vegetative organs. Photosystem II (PSII) maximum quantum yield was higher for transgenic seedlings than for non-transgenic seedlings, after either stress treatment. Furthermore, protection of both PSII and Photosystem I (PSI) membrane protein complexes was observed in the transgenic seedlings, leading to their survival after the stress treatments. It was also shown that the plastidial D1 protein, a labile component of the PSII reaction center, and the PSI core protein PsaB were shielded from oxidative damage and degradation. We infer that natural expression of the HSFA9 program during embryogenesis may protect seed pro-plastids from developmental desiccation.</p> </div>", "links"=>[], "tags"=>["photosynthetic", "apparatus", "dehydration", "oxidative", "seedlings", "transgenic", "tobacco"], "article_id"=>116383, "categories"=>["Biotechnology", "Cell Biology", "Genetics"], "users"=>["Concepción Almoguera", "Pilar Prieto-Dapena", "José-María Personat", "Javier Tejedor-Cano", "Marika Lindahl", "Antonio Diaz-Espejo", "Juan Jordano"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0051443.s001", "https://dx.doi.org/10.1371/journal.pone.0051443.s002", "https://dx.doi.org/10.1371/journal.pone.0051443.s003", "https://dx.doi.org/10.1371/journal.pone.0051443.s004"], "stats"=>{"downloads"=>7, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Protection_of_the_Photosynthetic_Apparatus_from_Extreme_Dehydration_and_Oxidative_Stress_in_Seedlings_of_Transgenic_Tobacco__/116383", "title"=>"Protection of the Photosynthetic Apparatus from Extreme Dehydration and Oxidative Stress in Seedlings of Transgenic Tobacco", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-12-05 01:46:23"}

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Relative Metric

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