Ultra-Bright and -Stable Red and Near-Infrared Squaraine Fluorophores for In Vivo Two-Photon Imaging
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{"title"=>"Ultra-Bright and -Stable Red and Near-Infrared Squaraine Fluorophores for In Vivo Two-Photon Imaging", "type"=>"journal", "authors"=>[{"first_name"=>"Kaspar", "last_name"=>"Podgorski", "scopus_author_id"=>"54941541900"}, {"first_name"=>"Ewald", "last_name"=>"Terpetschnig", "scopus_author_id"=>"6603379791"}, {"first_name"=>"Oleksii P.", "last_name"=>"Klochko", "scopus_author_id"=>"35189232100"}, {"first_name"=>"Olena M.", "last_name"=>"Obukhova", "scopus_author_id"=>"24468661600"}, {"first_name"=>"Kurt", "last_name"=>"Haas", "scopus_author_id"=>"55554765000"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84871288399", "pmid"=>"23251670", "isbn"=>"1932-6203 (Electronic)\r1932-6203 (Linking)", "pui"=>"366296438", "issn"=>"19326203", "scopus"=>"2-s2.0-84871288399", "doi"=>"10.1371/journal.pone.0051980"}, "id"=>"8a99a559-cb64-35bf-be62-2babc9ab3e94", "abstract"=>"Fluorescent dyes that are bright, stable, small, and biocompatible are needed for high-sensitivity two-photon imaging, but the combination of these traits has been elusive. We identified a class of squaraine derivatives with large two-photon action cross-sections (up to 10,000 GM) at near-infrared wavelengths critical for in vivo imaging. We demonstrate the biocompatibility and stability of a red-emitting squaraine-rotaxane (SeTau-647) by imaging dye-filled neurons in vivo over 5 days, and utility for sensitive subcellular imaging by synthesizing a specific peptide-conjugate label for the synaptic protein PSD-95.", "link"=>"http://www.mendeley.com/research/ultrabright-stable-red-nearinfrared-squaraine-fluorophores-vivo-twophoton-imaging", "reader_count"=>58, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>4, "Researcher"=>20, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>10, "Student > Postgraduate"=>4, "Student > Master"=>11, "Other"=>1, "Student > Bachelor"=>3, "Professor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>4, "Researcher"=>20, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>10, "Student > Postgraduate"=>4, "Student > Master"=>11, "Other"=>1, "Student > Bachelor"=>3, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>5, "Engineering"=>3, "Biochemistry, Genetics and Molecular Biology"=>6, "Materials Science"=>1, "Agricultural and Biological Sciences"=>18, "Medicine and Dentistry"=>2, "Neuroscience"=>5, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Physics and Astronomy"=>2, "Chemistry"=>13, "Computer Science"=>1, "Economics, Econometrics and Finance"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>3}, "Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Neuroscience"=>{"Neuroscience"=>5}, "Chemistry"=>{"Chemistry"=>13}, "Physics and Astronomy"=>{"Physics and Astronomy"=>2}, "Economics, Econometrics and Finance"=>{"Economics, Econometrics and Finance"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>18}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}, "Unspecified"=>{"Unspecified"=>5}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"Netherlands"=>2, "United States"=>2, "Israel"=>1, "Germany"=>1}, "group_count"=>1}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/282851"], "description"=>"<div><p>Fluorescent dyes that are bright, stable, small, and biocompatible are needed for high-sensitivity two-photon imaging, but the combination of these traits has been elusive. We identified a class of squaraine derivatives with large two-photon action cross-sections (up to 10,000 GM) at near-infrared wavelengths critical for <em>in vivo</em> imaging. We demonstrate the biocompatibility and stability of a red-emitting squaraine-rotaxane (SeTau-647) by imaging dye-filled neurons <em>in vivo</em> over 5 days, and utility for sensitive subcellular imaging by synthesizing a specific peptide-conjugate label for the synaptic protein PSD-95.</p> </div>", "links"=>[], "tags"=>["ultra-bright", "-stable", "near-infrared", "squaraine", "fluorophores", "two-photon", "imaging"], "article_id"=>115773, "categories"=>["Neuroscience", "Immunology"], "users"=>["Kaspar Podgorski", "Ewald Terpetschnig", "Oleksii P. Klochko", "Olena M. Obukhova", "Kurt Haas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0051980", "stats"=>{"downloads"=>3, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Ultra_Bright_and_Stable_Red_and_Near_Infrared_Squaraine_Fluorophores_for_In_Vivo_Two_Photon_Imaging__/115773", "title"=>"Ultra-Bright and -Stable Red and Near-Infrared Squaraine Fluorophores for <em>In Vivo</em> Two-Photon Imaging", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-12-14 01:36:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/524485"], "description"=>"<p><b>a)</b> Z-projection images acquired 3 hours (<b><i>left</i></b>) and 5 days (<b><i>right</i></b>) after single-cell electroporation with SeTau-647-dextran. Images were acquired at an excitation power of 10 mW at the back aperture of the objective (<8 mW at the focal plane). <b>b)</b> Targeted electroporation of a squaraine-tagged PDZ binding peptide labels postsynaptic density protein PSD-95. <i>(</i><b><i>top</i></b><i>)</i> Z-projection of a neuron filled with OGB-1 (<i>green</i>) and the squaraine-tagged peptide (<i>red</i>). <i>(</i><b><i>bottom</i></b><i>)</i> Expanded views of dendritic regions numbered above show punctate labelling of SeTau-647 (arrows). <b>c)</b> Anti-PSD-95 puncta colocalize with SeTau puncta in labelled tectal neuron dendrites. <i>(</i><b><i>left</i></b><i>)</i> Anti-PSD-95 immunostaining <i>(green)</i> of brain section containing dendrites of neurons labelled with SeTau PDZ-binding peptide <i>(red)</i> and Cascade Blue dextran <i>(blue)</i> as a space filler. <i>(</i><b><i>right</i></b><i>)</i> Expanded views of dendritic regions numbered at left. Scale bars: 10 µm.</p>", "links"=>[], "tags"=>["subcellular", "labelling", "setau-647"], "article_id"=>194982, "categories"=>["Neuroscience", "Immunology"], "users"=>["Kaspar Podgorski", "Ewald Terpetschnig", "Oleksii P. Klochko", "Olena M. Obukhova", "Kurt Haas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0051980.g003", "stats"=>{"downloads"=>0, "page_views"=>30, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cellular_and_subcellular_labelling_with_SeTau_647_in_vivo_/194982", "title"=>"Cellular and subcellular labelling with SeTau-647 <i>in vivo</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-12-14 01:23:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/524367"], "description"=>"<p><b>a)</b> Fluorescence emission spectrum of SeTau-647 (shaded curve) and two-photon action cross-section of SeTau-647, compared to published cross-sections <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051980#pone.0051980-Xu1\" target=\"_blank\">[30]</a> of a bright organic dye (Rhodamine B, red) and fluorescent protein (eGFP, green). For SeTau-647, data in closed circles were obtained by ratiometric fluorescence measurement, and data in open circles were obtained by Z-scan, which is less sensitive to single-photon phenomena. <b>b)</b> Simultaneous two-photon photobleaching of SeTau-647 and Alexa 488 at 920 nm in water. Dashed lines are fitted mono-exponential decay curves. The abscissa is the product of illumination time with the two-photon action cross-section of each dye, proportional to number of photons emitted per dye molecule. <b>c-e)</b> Photobleaching of SeTau-647 and Alexa 594 dextran conjugates in neuronal dendrites <i>in vivo</i>. <b>c)</b> Neurons were electroporated with 1 mM Alexa 594- (<b><i>top</i></b>) or SeTau-647- <i>(</i><b><i>bottom</i></b><i>)</i> dextran, and distal dendritic segments were imaged at the minimum laser intensity which allowed clear visualization. The 1<sup>st</sup> (<b><i>left</i></b>) and 60<sup>th</sup> (<b><i>right</i></b>) consecutive images are shown. <b>d)</b> Timecourse of photobleaching in example dendritic segments loaded with Alexa 594 (<i>red</i>) or SeTau-647 (<i>blue</i>) indicating. Lines are fitted monoexponential decay curves. Intensities have been normalized to the maximum and minimum of the fit curve. <b>e)</b> Distribution of fitted photobleaching rate (τ) for neurons loaded with Alexa 594 or SeTau-647. Each data point is the median fit bleaching rate over all dendritic segments imaged in one neuron. Horizontal bars denote the mean of each distribution.</p>", "links"=>[], "tags"=>["photostability", "squaraine-rotaxane", "setau-647"], "article_id"=>194859, "categories"=>["Neuroscience", "Immunology"], "users"=>["Kaspar Podgorski", "Ewald Terpetschnig", "Oleksii P. Klochko", "Olena M. Obukhova", "Kurt Haas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0051980.g002", "stats"=>{"downloads"=>1, "page_views"=>32, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Brightness_and_photostability_of_the_squaraine_rotaxane_SeTau_647_measured_in_vitro_and_in_vivo_/194859", "title"=>"Brightness and photostability of the squaraine-rotaxane SeTau-647 measured <i>in vitro</i> and <i>in vivo</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-12-14 01:20:59"}
  • {"files"=>["https://ndownloader.figshare.com/files/524236"], "description"=>"<p><b>a)</b> Structure of squaraine-rotaxanes. Squaraines contain a characteristic squarylium core flanked by nucleophilic motifs, forming an electron Donor-Acceptor-Donor structure. The macrocyclic ‘cage’ sterically shields the more reactive squarylium core, increasing its stability. <b>b)</b> Two-photon action cross-sections of squaraine derivatives. Dye names with a prefix of K8 denote squaraines, K9 prefixes denote squaraine-rotaxanes. Cross-sections were obtained by ratiometric imaging and calculated using emission spectra measured from BSA-conjugate (K8-1342), IgG-conjugate (K8-1384), or free dye (all others).</p>", "links"=>[], "tags"=>["derivatives", "two-photon", "cross-sections", "nir"], "article_id"=>194725, "categories"=>["Neuroscience", "Immunology"], "users"=>["Kaspar Podgorski", "Ewald Terpetschnig", "Oleksii P. Klochko", "Olena M. Obukhova", "Kurt Haas"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0051980.g001", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Squaraine_derivatives_with_large_two_photon_action_cross_sections_in_the_NIR_window_/194725", "title"=>"Squaraine derivatives with large two-photon action cross-sections in the NIR window.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-12-14 01:18:45"}

PMC Usage Stats | Further Information

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Relative Metric

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