MicroRNA-181 Regulates CARM1 and Histone Aginine Methylation to Promote Differentiation of Human Embryonic Stem Cells
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"MicroRNA-181 Regulates CARM1 and Histone Aginine Methylation to Promote Differentiation of Human Embryonic Stem Cells", "type"=>"journal", "authors"=>[{"first_name"=>"Zhenyu", "last_name"=>"Xu", "scopus_author_id"=>"55544651800"}, {"first_name"=>"Junfeng", "last_name"=>"Jiang", "scopus_author_id"=>"55544914800"}, {"first_name"=>"Chen", "last_name"=>"Xu", "scopus_author_id"=>"56263494800"}, {"first_name"=>"Yue", "last_name"=>"Wang", "scopus_author_id"=>"12799314000"}, {"first_name"=>"Lei", "last_name"=>"Sun", "scopus_author_id"=>"57110532500"}, {"first_name"=>"Xiaocan", "last_name"=>"Guo", "scopus_author_id"=>"55416643000"}, {"first_name"=>"Houqi", "last_name"=>"Liu", "scopus_author_id"=>"21933937900"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pmid"=>"23301034", "doi"=>"10.1371/journal.pone.0053146", "pui"=>"368044673", "issn"=>"19326203", "sgr"=>"84871904077", "scopus"=>"2-s2.0-84871904077"}, "id"=>"e24af373-edee-39f8-952c-256cdde971ce", "abstract"=>"As a novel epigenetic mechanism, histone H3 methylation at R17 and R26, which is mainly catalyzed by coactivator-associated protein arginine methyltransferase 1 (CARM1), has been reported to modulate the transcription of key pluripotency factors and to regulate pluripotency in mouse embryos and mouse embryonic stem cells (mESCs) in previous studies. However, the role of CARM1 in human embryonic stem cells (hESCs) and the regulatory mechanism that controls CARM1 expression during ESCs differentiation are presently unknown. Here, we demonstrate that CARM1 plays an active role in the resistance to differentiation in hESCs by regulating pluripotency genes in response to BMP4. In a functional screen, we identified the miR-181 family as a regulator of CARM1 that is induced during ESC differentiation and show that endogenous miR-181c represses the expression of CARM1. Depletion of CARM1 or enforced expression of miR-181c inhibits the expression of pluripotency genes and induces differentiation independent of BMP4, whereas overexpression of CARM1 or miR-181c inhibitor elevates Nanog and impedes differentiation. Furthermore, expression of CARM1 rescue constructs inhibits the effect of miR-181c overexpression in promoting differentiation. Taken together, our findings demonstrate the importance of a miR-181c-CARM1 pathway in regulating the differentiation of hESCs.", "link"=>"http://www.mendeley.com/research/microrna181-regulates-carm1-histone-aginine-methylation-promote-differentiation-human-embryonic-stem", "reader_count"=>20, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>4, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>5, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>4, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>5, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>6, "Agricultural and Biological Sciences"=>11, "Medicine and Dentistry"=>2}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>11}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}}, "reader_count_by_country"=>{"United States"=>1, "Germany"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84871904077"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84871904077?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84871904077&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84871904077&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84871904077", "dc:identifier"=>"SCOPUS_ID:84871904077", "eid"=>"2-s2.0-84871904077", "dc:title"=>"MicroRNA-181 Regulates CARM1 and Histone Aginine Methylation to Promote Differentiation of Human Embryonic Stem Cells", "dc:creator"=>"Xu Z.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"8", "prism:issueIdentifier"=>"1", "prism:pageRange"=>nil, "prism:coverDate"=>"2013-01-03", "prism:coverDisplayDate"=>"3 January 2013", "prism:doi"=>"10.1371/journal.pone.0053146", "citedby-count"=>"33", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Second Military Medical University", "affiliation-city"=>"Shanghai", "affiliation-country"=>"China"}], "pubmed-id"=>"23301034", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e53146", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0053146", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"101", "xml_views"=>"1", "html_views"=>"264"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"27", "xml_views"=>"3", "html_views"=>"75"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"28", "xml_views"=>"1", "html_views"=>"93"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"20", "xml_views"=>"2", "html_views"=>"69"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"26", "xml_views"=>"1", "html_views"=>"69"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"97"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"44"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"12", "xml_views"=>"3", "html_views"=>"55"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"4", "html_views"=>"61"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"34"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"66"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"41"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"28"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"31"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"23", "xml_views"=>"2", "html_views"=>"20"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"46", "xml_views"=>"2", "html_views"=>"17"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"18", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"3", "html_views"=>"5"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"8"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"8"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"5"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"0"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"0"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"5"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/278876", "https://ndownloader.figshare.com/files/278901", "https://ndownloader.figshare.com/files/278932", "https://ndownloader.figshare.com/files/278979", "https://ndownloader.figshare.com/files/279014", "https://ndownloader.figshare.com/files/279078"], "description"=>"<div><p>As a novel epigenetic mechanism, histone H3 methylation at R17 and R26, which is mainly catalyzed by coactivator-associated protein arginine methyltransferase 1 (CARM1), has been reported to modulate the transcription of key pluripotency factors and to regulate pluripotency in mouse embryos and mouse embryonic stem cells (mESCs) in previous studies. However, the role of CARM1 in human embryonic stem cells (hESCs) and the regulatory mechanism that controls <em>CARM1</em> expression during ESCs differentiation are presently unknown. Here, we demonstrate that CARM1 plays an active role in the resistance to differentiation in hESCs by regulating pluripotency genes in response to BMP4. In a functional screen, we identified the miR-181 family as a regulator of <em>CARM1</em> that is induced during ESC differentiation and show that endogenous miR-181c represses the expression of <em>CARM1</em>. Depletion of CARM1 or enforced expression of miR-181c inhibits the expression of pluripotency genes and induces differentiation independent of BMP4, whereas overexpression of <em>CARM1</em> or miR-181c inhibitor elevates Nanog and impedes differentiation. Furthermore, expression of <em>CARM1</em> rescue constructs inhibits the effect of miR-181c overexpression in promoting differentiation. Taken together, our findings demonstrate the importance of a miR-181c-CARM1 pathway in regulating the differentiation of hESCs.</p> </div>", "links"=>[], "tags"=>["microrna-181", "regulates", "carm1", "histone", "aginine", "methylation", "differentiation", "embryonic", "cells"], "article_id"=>114996, "categories"=>["Molecular Biology", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Zhenyu Xu", "Junfeng Jiang", "Chen Xu", "Yue Wang", "Lei Sun", "Xiaocan Guo", "Houqi Liu"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0053146.s001", "https://dx.doi.org/10.1371/journal.pone.0053146.s002", "https://dx.doi.org/10.1371/journal.pone.0053146.s003", "https://dx.doi.org/10.1371/journal.pone.0053146.s004", "https://dx.doi.org/10.1371/journal.pone.0053146.s005", "https://dx.doi.org/10.1371/journal.pone.0053146.s006"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/MicroRNA_181_Regulates_CARM1_and_Histone_Aginine_Methylation_to_Promote_Differentiation_of_Human_Embryonic_Stem_Cells__/114996", "title"=>"MicroRNA-181 Regulates CARM1 and Histone Aginine Methylation to Promote Differentiation of Human Embryonic Stem Cells", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-01-03 01:23:16"}
  • {"files"=>["https://ndownloader.figshare.com/files/513419"], "description"=>"<p>(A, B) The kinetic expression levels of <i>CARM1</i>, <i>Nanog</i>, <i>Sox2</i>, and <i>Oct4</i> in differentiated hESCs. Human ESCs differentiation was induced by the addition of BMP4 in the absence of bFGF. The expression levels of <i>CARM1</i>, <i>Nanog</i>, <i>Sox2</i>, and <i>Oct4</i> at the mRNA (A) and protein levels (B) were quantified by qRT-PCR and Western blotting, respectively. *, p<0.05; **, p<0.01. (C, D) Overexpression of 3′UTR-deficient <i>CARM1</i> rescued the down-regulation of key pluripotency factors during hESC differentiation. Differentiation of hESCs overexpressing 3′UTR-deficient <i>CARM1</i> was induced, and the expression levels of <i>CARM1</i>, <i>Nanog</i>, <i>Sox2</i>, and <i>Oct4</i> at the mRNA (C) and protein levels (D) were quantified by qRT-PCR and Western blotting, respectively. Blank pcDNA3 was used as a negative control. Samples were assayed in duplicate (n = 3) and normalized to endogenous <i>β-actin</i> expression. **, p<0.01. (E) Knockdown of <i>CARM1</i> in ESCs using siRNAs resulted in the down-regulation of <i>CARM1</i>, <i>Nanog, Sox2</i> and <i>Oct4</i> mRNA levels. <i>CARM1</i>-specific siRNAs were transfected every 4 days; total RNA was extracted from hESCs 8 days after the first transfection and quantified by quantitative real-time polymerase chain reaction (qRT-PCR). Mean values of the indicated transcript levels are plotted as percentages relative to those after transfection with negative control RNA (NC RNA). Samples were assayed in duplicate (n = 3) and normalized to endogenous <i>β-actin</i> expression. **, p<0.01. (F) The pluripotency of hESCs was examined by AP staining 8 days after siRNA transfection. NC RNA-transfected ESCs were used as controls. The counts of AP-positive clones per field were quantified in duplicate (n = 3), and the images for the representative plates are all shown. **, p<0.01.</p>", "links"=>[], "tags"=>["down-regulated", "post-transcriptionally", "hesc"], "article_id"=>183911, "categories"=>["Molecular Biology", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Zhenyu Xu", "Junfeng Jiang", "Chen Xu", "Yue Wang", "Lei Sun", "Xiaocan Guo", "Houqi Liu"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0053146.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_CARM1_is_down_regulated_post_transcriptionally_during_hESC_differentiation_/183911", "title"=>"CARM1 is down-regulated post-transcriptionally during hESC differentiation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-03 01:05:11"}
  • {"files"=>["https://ndownloader.figshare.com/files/513504"], "description"=>"<p>(A) The expression levels of mature miRNAs predicted to target the <i>CARM1</i> 3′UTR were monitored in differentiated ESCs by qRT-PCR and normalized to endogenous <i>U6</i> expression. *, p<0.05; **, p<0.01. (B) The chromosome positions of the primary transcripts of miR-181 family members are shown, their expression levels in differentiated ESCs were determined by qRT-PCR and normalized to endogenous <i>β-actin</i> expression. **, p<0.01. (C) The predicted consequential pairing of miRNAs and their target regions in the wild-type <i>CARM1</i> 3′UTR or the mutant (mut) <i>CARM1</i> 3′UTR are shown. (D) The HEK293 cells were co-transfected with the firefly-luciferase-expressing vector pMIR-REPORT containing the wild-type <i>CARM1</i> 3′UTR, the mut <i>CARM1</i> 3′UTR or the control insert as well as the internal control renilla-luciferase-expressing vector pRL-TK and the indicated RNAs. After 48 h, the luciferase activities were measured. The data were normalized by dividing firefly luciferase activity with that of Renilla luciferase, **, p<0.01. (D) Luciferase activities were measured in human ESCs co-transfected with the pMIR-REPORT plasmid containing wild-type <i>CARM1</i> 3′UTR, mut <i>CARM1</i> 3′UTR or control insert and the internal control, pRL-TK. All the samples were assayed in duplicate (n = 3). **, p<0.01.</p>", "links"=>[], "tags"=>["mir-181", "regulates"], "article_id"=>183997, "categories"=>["Molecular Biology", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Zhenyu Xu", "Junfeng Jiang", "Chen Xu", "Yue Wang", "Lei Sun", "Xiaocan Guo", "Houqi Liu"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0053146.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_miR_181_family_directly_regulates_CARM1_expression_in_hESC_/183997", "title"=>"The miR-181 family directly regulates <i>CARM1</i> expression in hESC.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-03 01:06:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/513597"], "description"=>"<p>(A) Overexpression of miR-181c down-regulated <i>CARM1</i> expression in comparison to negative control (NC) RNA-transfected ESCs at both the mRNA and protein levels, as determined by qRT-PCR and Western blotting, respectively. H3R17 methylation level and <i>Oct4</i>, <i>Nanog</i> and <i>Sox2</i> mRNA expression were also monitored. Samples were assayed in duplicate (n = 3) and normalized to endogenous <i>β-actin</i> expression. **, p<0.01. (B) Expression of a subset of differentiation genes in ESCs that were transfected with miR-181c or NC RNA or were not transfected was monitored by qRT-PCR. Mean levels (after 3 days) of expression are shown relative to the NC RNA-transfected sample (shown as one fold) and normalized to <i>β-actin</i> expression levels. **, p<0.01. (C, D, E) miR-181c mimics were transfected into ESCs with the 3′UTR-deficient-<i>CARM1</i>-expressing plasmid (<i>CARM1</i> OE) or the control plasmid (pcDNA3). ESCs co-transfected with NC RNA and pcDNA3 were used as controls. (C) Pluripotency was examined by AP staining 8 days after transfection. The images for the whole plate or representative clones are shown. Scale bar: 500 µm. CARM1 protein expression is also shown. **, p<0.01. (D) Expression of a subset of differentiation genes in ESCs transfected with miR-181c+pcDNA3 or miR-181c+<i>CARM1</i> or NC RNA was monitored by qRT-PCR. Mean levels (after 3 days) of expression are shown relative to the NC RNA-transfected sample (shown as one fold) and normalized to <i>β-actin</i> expression levels. (E) <i>Oct4</i>, <i>Nanog</i> and <i>Sox2</i> mRNA expression was also monitored by qRT-PCR. Samples were assayed in duplicate (n = 3) and normalized to endogenous <i>β-actin</i> expression. **, p<0.01. (F) ChIP analysis of hESCs overexpressing <i>CARM1</i> or miR-181c. ChIP was performed on sonicated chromatin using anti-CARM1 or anti-H3R17-di-me antibodies. Immunoprecipitated DNA was analyzed by qRT-PCR with primers targeting the promoter regions of the investigated gene. The fold enrichment value is shown as the normalized ChIP signal divided by the normalized input signal. Cells transfected with NC RNA or pcDNA3 were used as negative controls. The results of the electrophoretic analysis are also shown in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053146#pone.0053146.s003\" target=\"_blank\">Fig. S3A</a>. *, p<0.05; **, p<0.01.</p>", "links"=>[], "tags"=>["leads", "hesc", "differentiation", "h3r17"], "article_id"=>184089, "categories"=>["Molecular Biology", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Zhenyu Xu", "Junfeng Jiang", "Chen Xu", "Yue Wang", "Lei Sun", "Xiaocan Guo", "Houqi Liu"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0053146.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_miR_181c_leads_to_hESC_differentiation_through_negative_regulation_of_CARM1_and_H3R17_methylation_/184089", "title"=>"miR-181c leads to hESC differentiation through negative regulation of <i>CARM1</i> and H3R17 methylation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-03 01:08:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/513700"], "description"=>"<p>(A) Enforced expression of the miR-181c inhibitor down-regulated mature miR-181c levels relative to negative control (NC) RNA-transfected ESCs, as shown by qRT-PCR. **, p<0.01. Western blotting detected that CARM1 and H3R17me2 protein levels were clearly decreased. (B, C, D) The effect of miR-181c inhibition on differentiated hESCs. Human ESCs were transfected with miR-181c inhibitor or NC RNA and then induced to differentiate by the addition of BMP4 in the absence of bFGF. (B) The expression of <i>CARM1</i>, <i>Nanog</i>, <i>Sox2</i>, and <i>Oct4</i> at the mRNA level was quantified by qRT-PCR, CARM1 and Nanog protein expression levels were also quantified by Western blotting. (C) Pluripotency was examined by AP staining 3 days after transfection. The counts of AP-positive clones and the images of the representative plates are shown. Samples were assayed in duplicate (n = 3). **, p<0.01. (D) Expression of a subset of differentiation-associated genes in ESCs transfected with miR-181c inhibitor or NC RNA were monitored by qRT-PCR and normalized to <i>β-actin</i> expression levels. Mean levels (after 8 days) expressed relative to undifferentiated hESCs (shown as one fold) are shown. (E) Model for the miR-181/CARM1/core-pluripotency-factors regulatory loop in the modulation of hESC pluripotency. Pluripotency is maintained in ESCs in part by histone H3 arginine methylation by CARM1 at the <i>Oct4</i>, <i>Nanog</i> and <i>Sox2</i> promoters. The core pluripotency factors also recruit H3K27 methylases to the miR-181c promoter to inhibit its expression. In differentiated hESCs, H3K27 methylation is inhibited due to the reduction of core pluripotency factors, and miR-181 family members are subsequently induced and down-regulate CARM1 activity. H3R17me2 production is eventually stopped, which aggravates the decrease in expression of core pluripotency factors as well as the loss of pluripotency.</p>", "links"=>[], "tags"=>["mir-181c", "inhibitor", "suppresses", "hesc"], "article_id"=>184190, "categories"=>["Molecular Biology", "Cell Biology", "Genetics", "Developmental Biology"], "users"=>["Zhenyu Xu", "Junfeng Jiang", "Chen Xu", "Yue Wang", "Lei Sun", "Xiaocan Guo", "Houqi Liu"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0053146.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_miR_181c_inhibitor_suppresses_hESC_differentiation_/184190", "title"=>"The miR-181c inhibitor suppresses hESC differentiation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-03 01:09:50"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"73", "full-text"=>"71", "pdf"=>"29", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"43", "supp-data"=>"47", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"40", "full-text"=>"38", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"40", "full-text"=>"35", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"29", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"40", "full-text"=>"47", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"11", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"22", "full-text"=>"21", "pdf"=>"6", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"24", "full-text"=>"16", "pdf"=>"16", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"19", "full-text"=>"20", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"15", "full-text"=>"11", "pdf"=>"6", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"20", "full-text"=>"20", "pdf"=>"22", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"19", "pdf"=>"39", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"32", "full-text"=>"35", "pdf"=>"42", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"15", "full-text"=>"12", "pdf"=>"39", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"14", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"23", "full-text"=>"22", "pdf"=>"12", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"7", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"8", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"5", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"18", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"18", "full-text"=>"17", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"19", "full-text"=>"19", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"24", "full-text"=>"16", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"12", "pdf"=>"5", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"18", "full-text"=>"16", "pdf"=>"6", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"13", "full-text"=>"10", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"2", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"16", "full-text"=>"22", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"13", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"11", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"18", "full-text"=>"16", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"35", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"39", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"15", "full-text"=>"21", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"50", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"20", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"32", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"7", "cited-by"=>"3", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}

Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Developmental biology", "average_usage"=>[275, 472, 605, 720, 822, 921, 1013, 1106, 1200, 1289, 1378, 1459, 1531]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[284, 491, 620, 738, 843, 945, 1043, 1137, 1225, 1315, 1400, 1479, 1555]}, {"subject_area"=>"/Physical sciences", "average_usage"=>[254, 431, 547, 651, 748, 842, 932, 1017, 1098, 1178, 1259, 1336, 1404]}]}
Loading … Spinner
There are currently no alerts