The Cancer Stem Cell Marker CD133 Interacts with Plakoglobin and Controls Desmoglein-2 Protein Levels
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"The Cancer Stem Cell Marker CD133 Interacts with Plakoglobin and Controls Desmoglein-2 Protein Levels", "type"=>"journal", "authors"=>[{"first_name"=>"Ryo", "last_name"=>"Koyama-Nasu", "scopus_author_id"=>"8734689900"}, {"first_name"=>"Rina", "last_name"=>"Takahashi", "scopus_author_id"=>"55554425700"}, {"first_name"=>"Satoshi", "last_name"=>"Yanagida", "scopus_author_id"=>"37001258700"}, {"first_name"=>"Yukiko", "last_name"=>"Nasu-Nishimura", "scopus_author_id"=>"13607434000"}, {"first_name"=>"Masaaki", "last_name"=>"Oyama", "scopus_author_id"=>"7103070270"}, {"first_name"=>"Hiroko", "last_name"=>"Kozuka-Hata", "scopus_author_id"=>"7801447915"}, {"first_name"=>"Ryo", "last_name"=>"Haruta", "scopus_author_id"=>"55352238700"}, {"first_name"=>"Emi", "last_name"=>"Manabe", "scopus_author_id"=>"55352055900"}, {"first_name"=>"Akemi", "last_name"=>"Hoshino-Okubo", "scopus_author_id"=>"55554558800"}, {"first_name"=>"Hiroko", "last_name"=>"Omi", "scopus_author_id"=>"24279159900"}, {"first_name"=>"Nozomu", "last_name"=>"Yanaihara", "scopus_author_id"=>"8696702400"}, {"first_name"=>"Aikou", "last_name"=>"Okamoto", "scopus_author_id"=>"7201644103"}, {"first_name"=>"Tadao", "last_name"=>"Tanaka", "scopus_author_id"=>"8060664800"}, {"first_name"=>"Tetsu", "last_name"=>"Akiyama", "scopus_author_id"=>"7403024170"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84872221587", "sgr"=>"84872221587", "issn"=>"19326203", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pmid"=>"23326490", "doi"=>"10.1371/journal.pone.0053710", "pui"=>"368101298"}, "id"=>"02a934c9-bb8b-3a89-bd1e-34a72ca54a29", "abstract"=>"The pentaspan membrane glycoprotein CD133 (also known as prominin-1) has been widely used as a marker for both cancer and normal stem cells. However, the function of CD133 has not been elucidated. Here we describe a cancer stem cell line established from clear cell carcinoma of the ovary (CCC) and show that CD133 interacts with plakoglobin (also known as γ-catenin), a desmosomal linker protein. We further demonstrate that knockdown of CD133 by RNA interference (RNAi) results in the downregulation of desmoglein-2, a desmosomal cadherin, and abrogates cell-cell adhesion and tumorigenicity of CCC stem cells. We speculate that CD133 may be a promising target for cancer chemotherapy.", "link"=>"http://www.mendeley.com/research/cancer-stem-cell-marker-cd133-interacts-plakoglobin-controls-desmoglein2-protein-levels", "reader_count"=>26, "reader_count_by_academic_status"=>{"Researcher"=>4, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>11, "Student > Postgraduate"=>2, "Student > Master"=>5, "Student > Bachelor"=>1, "Lecturer"=>1}, "reader_count_by_user_role"=>{"Researcher"=>4, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>11, "Student > Postgraduate"=>2, "Student > Master"=>5, "Student > Bachelor"=>1, "Lecturer"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>3, "Agricultural and Biological Sciences"=>16, "Medicine and Dentistry"=>7}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>7}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>16}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}}, "reader_count_by_country"=>{"United States"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84872221587"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84872221587?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84872221587&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84872221587&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84872221587", "dc:identifier"=>"SCOPUS_ID:84872221587", "eid"=>"2-s2.0-84872221587", "dc:title"=>"The Cancer Stem Cell Marker CD133 Interacts with Plakoglobin and Controls Desmoglein-2 Protein Levels", "dc:creator"=>"Koyama-Nasu R.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"8", "prism:issueIdentifier"=>"1", "prism:pageRange"=>nil, "prism:coverDate"=>"2013-01-17", "prism:coverDisplayDate"=>"17 January 2013", "prism:doi"=>"10.1371/journal.pone.0053710", "citedby-count"=>"7", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"University of Tokyo", "affiliation-city"=>"Tokyo", "affiliation-country"=>"Japan"}], "pubmed-id"=>"23326490", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e53710", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0053710", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"79", "xml_views"=>"11", "html_views"=>"308"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"33", "xml_views"=>"2", "html_views"=>"97"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"86"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"23", "xml_views"=>"3", "html_views"=>"79"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"71"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"58"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"12", "xml_views"=>"2", "html_views"=>"36"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"36"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"52"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"2", "html_views"=>"27"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"34"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"36"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"25"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"18"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"16"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"60"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"3", "html_views"=>"6"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"2"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/509635"], "description"=>"<p>(A) CCC stem and differentiated (diff) cells in culture. Phase contrast photographs are shown. (B) The mRNA levels of the indicated genes were evaluated by quantitative RT-PCR and shown as fold change over mRNA levels in CCC stem cells. Error bars represent the s.d. (<i>n</i> = 3). (C) CCC stem or differentiated cells were subcutaneously transplanted into NOG mice (<i>n</i> = 3). Seven months after transplantation, mice (upper) and tumors (lower) were photographed. (D) Elutes from immunopurified CD133 from the membrane fraction of CCC stem cells were resolved by SDS-PAGE and visualized by silver staining. (E) Desmosomal proteins identified by mass spectrometry. The numbers of unique peptides identified are shown. (F) Samples were prepared as described in (D) and immunoblotted with antibodies to the indicated proteins. (G) Co-localization of CD133 (red) with desmosomal proteins (green). CCC stem cells were immunostained with antibodies to the indicated proteins. TO-PRO-3 iodide was used for nuclear DNA staining (blue). Scale bars represent 20 µm.</p>", "links"=>[], "tags"=>["interacts", "plakoglobin", "localizes", "regions", "cell-cell", "ccc"], "article_id"=>180104, "categories"=>["Cancer", "Cell Biology", "Developmental Biology"], "users"=>["Ryo Koyama-Nasu", "Rina Takahashi", "Satoshi Yanagida", "Yukiko Nasu-Nishimura", "Masaaki Oyama", "Hiroko Kozuka-Hata", "Ryo Haruta", "Emi Manabe", "Akemi Hoshino-Okubo", "Hiroko Omi", "Nozomu Yanaihara", "Aikou Okamoto", "Tadao Tanaka", "Tetsu Akiyama"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0053710.g001", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_CD133_interacts_with_plakoglobin_and_localizes_specifically_to_regions_of_cell_cell_contact_in_CCC_stem_cells_/180104", "title"=>"CD133 interacts with plakoglobin and localizes specifically to regions of cell-cell contact in CCC stem cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-10 00:01:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/509789"], "description"=>"<p>(A) CCC stem cells were infected with a lentivirus expressing an shRNA targeting CD133. Cells were subjected to mechanical stress by pipetting in PBS containing 1 mM CaCl<sub>2</sub> and 0.5 mM MgCl<sub>2</sub>. Representative images are shown (upper). The bar graph represents the ratio of cell number/cluster number (lower). Error bars represent the s.d. (<i>n</i> = 3). <i>p</i> = 0.011 with comparison to control shRNA by t test. (B) CCC stem cells were treated as described in (A). Cell lysates were subjected to immunoblotting with antibodies to the indicated proteins. (C) CCC stem cells were treated as described in (A). Cells were immunostained with antibodies to the indicated proteins (red). TO-PRO-3 iodide was used for nuclear DNA staining (blue). Scale bars represent 20 µm. (D) CCC stem cells were treated as described in (A). Cells were subcutaneously transplanted into immunocompromised mice (<i>n</i> = 3). Eleven months after transplantation, mice (upper) and tumors (middle) were photographed. The bar graph represents tumor weight (lower). Error bars represent the s.d. (<i>n</i> = 3). <i>p</i> = 0.007 with comparison to control shRNA by t test.</p>", "links"=>[], "tags"=>["controls", "cell-cell", "adhesion", "tumorigenicity", "ccc"], "article_id"=>180261, "categories"=>["Cancer", "Cell Biology", "Developmental Biology"], "users"=>["Ryo Koyama-Nasu", "Rina Takahashi", "Satoshi Yanagida", "Yukiko Nasu-Nishimura", "Masaaki Oyama", "Hiroko Kozuka-Hata", "Ryo Haruta", "Emi Manabe", "Akemi Hoshino-Okubo", "Hiroko Omi", "Nozomu Yanaihara", "Aikou Okamoto", "Tadao Tanaka", "Tetsu Akiyama"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0053710.g002", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_CD133_controls_cell_cell_adhesion_and_is_required_for_tumorigenicity_of_CCC_stem_cells_/180261", "title"=>"CD133 controls cell-cell adhesion and is required for tumorigenicity of CCC stem cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-10 00:04:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/277650", "https://ndownloader.figshare.com/files/277679", "https://ndownloader.figshare.com/files/277726", "https://ndownloader.figshare.com/files/277771", "https://ndownloader.figshare.com/files/277809"], "description"=>"<div><p>The pentaspan membrane glycoprotein CD133 (also known as prominin-1) has been widely used as a marker for both cancer and normal stem cells. However, the function of CD133 has not been elucidated. Here we describe a cancer stem cell line established from clear cell carcinoma of the ovary (CCC) and show that CD133 interacts with plakoglobin (also known as γ-catenin), a desmosomal linker protein. We further demonstrate that knockdown of CD133 by RNA interference (RNAi) results in the downregulation of desmoglein-2, a desmosomal cadherin, and abrogates cell-cell adhesion and tumorigenicity of CCC stem cells. We speculate that CD133 may be a promising target for cancer chemotherapy.</p> </div>", "links"=>[], "tags"=>["cancer", "cd133", "interacts", "plakoglobin", "controls", "desmoglein-2", "levels"], "article_id"=>114755, "categories"=>["Cancer", "Cell Biology", "Developmental Biology"], "users"=>["Ryo Koyama-Nasu", "Rina Takahashi", "Satoshi Yanagida", "Yukiko Nasu-Nishimura", "Masaaki Oyama", "Hiroko Kozuka-Hata", "Ryo Haruta", "Emi Manabe", "Akemi Hoshino-Okubo", "Hiroko Omi", "Nozomu Yanaihara", "Aikou Okamoto", "Tadao Tanaka", "Tetsu Akiyama"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0053710.s001", "https://dx.doi.org/10.1371/journal.pone.0053710.s002", "https://dx.doi.org/10.1371/journal.pone.0053710.s003", "https://dx.doi.org/10.1371/journal.pone.0053710.s004", "https://dx.doi.org/10.1371/journal.pone.0053710.s005"], "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/The_Cancer_Stem_Cell_Marker_CD133_Interacts_with_Plakoglobin_and_Controls_Desmoglein_2_Protein_Levels__/114755", "title"=>"The Cancer Stem Cell Marker CD133 Interacts with Plakoglobin and Controls Desmoglein-2 Protein Levels", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-01-10 01:19:15"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"53", "full-text"=>"57", "pdf"=>"26", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"33", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"37", "full-text"=>"42", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"35", "full-text"=>"27", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"26", "full-text"=>"27", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"25", "full-text"=>"21", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"21", "full-text"=>"17", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"23", "full-text"=>"29", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"5", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"6", "pdf"=>"2", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"5", "pdf"=>"8", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"14", "full-text"=>"9", "pdf"=>"6", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"8", "pdf"=>"6", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"9", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"0", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"10", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"1", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"13", "full-text"=>"8", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}

Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[269, 466, 588, 697, 800, 896, 988, 1076, 1165, 1254, 1340, 1417]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[272, 472, 600, 713, 815, 911, 1004, 1094, 1185, 1273, 1358, 1441]}, {"subject_area"=>"/Biology and life sciences/Developmental biology", "average_usage"=>[275, 472, 605, 720, 822, 921, 1013, 1106, 1200, 1289, 1378, 1459, 1531]}, {"subject_area"=>"/Biology and life sciences/Microbiology", "average_usage"=>[293, 503, 638, 755, 861, 960, 1056, 1146, 1239, 1323, 1403, 1491, 1568]}, {"subject_area"=>"/Medicine and health sciences/Clinical genetics", "average_usage"=>[270, 469, 620, 749, 851, 945, 1042, 1126, 1248, 1337, 1421, 1478, 1570]}, {"subject_area"=>"/Medicine and health sciences/Surgical and invasive medical procedures", "average_usage"=>[223, 396, 511, 599, 690, 775, 859, 936, 1012, 1090, 1168, 1237, 1299]}]}
Loading … Spinner
There are currently no alerts