ATP Mediates NADPH Oxidase/ROS Generation and COX-2/PGE2 Expression in A549 Cells: Role of P2 Receptor-Dependent STAT3 Activation
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"ATP Mediates NADPH Oxidase/ROS Generation and COX-2/PGE2 Expression in A549 Cells: Role of P2 Receptor-Dependent STAT3 Activation", "type"=>"journal", "authors"=>[{"first_name"=>"Shin Ei", "last_name"=>"Cheng", "scopus_author_id"=>"9234034600"}, {"first_name"=>"I. Ta", "last_name"=>"Lee", "scopus_author_id"=>"35196417900"}, {"first_name"=>"Chih Chung", "last_name"=>"Lin", "scopus_author_id"=>"16025187700"}, {"first_name"=>"Wan Ling", "last_name"=>"Wu", "scopus_author_id"=>"55937129200"}, {"first_name"=>"Li Der", "last_name"=>"Hsiao", "scopus_author_id"=>"7005518270"}, {"first_name"=>"Chuen Mao", "last_name"=>"Yang", "scopus_author_id"=>"7407030244"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84872221711", "sgr"=>"84872221711", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0054125", "pmid"=>"23326583", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pui"=>"368101443"}, "id"=>"b645830d-a032-3751-8497-d93d6d845316", "abstract"=>"BACKGROUND: Up-regulation of cyclooxygenase (COX)-2 and its metabolite prostaglandin E(2) (PGE(2)) are frequently implicated in lung inflammation. Extracellular nucleotides, such as ATP have been shown to act via activation of P2 purinoceptors, leading to COX-2 expression in various inflammatory diseases, such as lung inflammation. However, the mechanisms underlying ATP-induced COX-2 expression and PGE(2) release remain unclear.\\n\\nPRINCIPAL FINDINGS: Here, we showed that ATPγS induced COX-2 expression in A549 cells revealed by western blot and real-time PCR. Pretreatment with the inhibitors of P2 receptor (PPADS and suramin), PKC (Gö6983, Gö6976, Ro318220, and Rottlerin), ROS (Edaravone), NADPH oxidase [diphenyleneiodonium chloride (DPI) and apocynin], Jak2 (AG490), and STAT3 [cucurbitacin E (CBE)] and transfection with siRNAs of PKCα, PKCι, PKCμ, p47(phox), Jak2, STAT3, and cPLA(2) markedly reduced ATPγS-induced COX-2 expression and PGE(2) production. In addition, pretreatment with the inhibitors of P2 receptor attenuated PKCs translocation from the cytosol to the membrane in response to ATPγS. Moreover, ATPγS-induced ROS generation and p47(phox) translocation was also reduced by pretreatment with the inhibitors of P2 receptor, PKC, and NADPH oxidase. On the other hand, ATPγS stimulated Jak2 and STAT3 activation which were inhibited by pretreatment with PPADS, suramin, Gö6983, Gö6976, Ro318220, GF109203X, Rottlerin, Edaravone, DPI, and apocynin in A549 cells.\\n\\nSIGNIFICANCE: Taken together, these results showed that ATPγS induced COX-2 expression and PGE(2) production via a P2 receptor/PKC/NADPH oxidase/ROS/Jak2/STAT3/cPLA(2) signaling pathway in A549 cells. Increased understanding of signal transduction mechanisms underlying COX-2 gene regulation will create opportunities for the development of anti-inflammation therapeutic strategies.", "link"=>"http://www.mendeley.com/research/atp-mediates-nadph-oxidaseros-generation-cox2pge2-expression-a549-cells-role-p2-receptordependent-st", "reader_count"=>37, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>3, "Researcher"=>3, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>10, "Student > Postgraduate"=>4, "Other"=>2, "Student > Master"=>6, "Student > Bachelor"=>3, "Professor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>3, "Researcher"=>3, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>10, "Student > Postgraduate"=>4, "Other"=>2, "Student > Master"=>6, "Student > Bachelor"=>3, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>6, "Agricultural and Biological Sciences"=>20, "Medicine and Dentistry"=>4, "Neuroscience"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>2, "Chemistry"=>2, "Psychology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Neuroscience"=>{"Neuroscience"=>1}, "Chemistry"=>{"Chemistry"=>2}, "Psychology"=>{"Psychology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>20}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}, "Unspecified"=>{"Unspecified"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>2}}, "reader_count_by_country"=>{"Iran"=>1, "Chile"=>2}, "group_count"=>2}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84872221711"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84872221711?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84872221711&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84872221711&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84872221711", "dc:identifier"=>"SCOPUS_ID:84872221711", "eid"=>"2-s2.0-84872221711", "dc:title"=>"ATP Mediates NADPH Oxidase/ROS Generation and COX-2/PGE<inf>2</inf> Expression in A549 Cells: Role of P2 Receptor-Dependent STAT3 Activation", "dc:creator"=>"Cheng S.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"8", "prism:issueIdentifier"=>"1", "prism:pageRange"=>nil, "prism:coverDate"=>"2013-01-17", "prism:coverDisplayDate"=>"17 January 2013", "prism:doi"=>"10.1371/journal.pone.0054125", "citedby-count"=>"32", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Chang Gung University", "affiliation-city"=>"Taoyuan", "affiliation-country"=>"Taiwan"}], "pubmed-id"=>"23326583", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e54125", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0054125", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"61", "xml_views"=>"1", "html_views"=>"144"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"34", "xml_views"=>"2", "html_views"=>"85"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"78"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"24", "xml_views"=>"3", "html_views"=>"82"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"81"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"87"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"96"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"83"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"100"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"27", "xml_views"=>"7", "html_views"=>"114"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"21", "xml_views"=>"2", "html_views"=>"116"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"99"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"81"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"19", "xml_views"=>"1", "html_views"=>"88"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"2", "html_views"=>"124"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"142"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"110"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"25", "xml_views"=>"1", "html_views"=>"132"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"21", "xml_views"=>"3", "html_views"=>"89"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"24", "xml_views"=>"1", "html_views"=>"137"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"82"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"3", "html_views"=>"56"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"74"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"3", "html_views"=>"35"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"25"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"3", "html_views"=>"20"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"3", "html_views"=>"39"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"10"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"19"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"4"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"3", "html_views"=>"14"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"12"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"4"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/510246"], "description"=>"<p>(A) Cells were treated with ATPγS for the indicated times. The expression of cPLA<sub>2</sub>, COX-2, or COX-1 was determined by Western blot. (B) Cells were transfected with cPLA<sub>2</sub> or COX-2 siRNA, and then treated with ATPγS for 24 h or 6 h. The expression of cPLA<sub>2</sub> or COX-2 was determined by Western blot. (C) Cells were treated with AA for the indicated times. The expression of COX-2 or COX-1 was determined by Western blot. (D) Cells were treated with ATPγS or AA for the indicated times. The production of PGE<sub>2</sub> was measured. (E) Cells were transfected with cPLA<sub>2</sub> or COX-2 siRNA, and then treated with ATPγS for 6 h. The production of PGE<sub>2</sub> was measured. Data are expressed as mean±S.E.M. of three independent experiments. <sup>#</sup><i>p</i><0.01, as compared with the cells exposed to vehicle alone (D) or scrambled siRNA+ATPγS (E).</p>", "links"=>[], "tags"=>["induces", "cox-2"], "article_id"=>180736, "categories"=>["Immunology"], "users"=>["Shin-Ei Cheng", "I-Ta Lee", "Chih-Chung Lin", "Wan-Ling Wu", "Li-Der Hsiao", "Chuen-Mao Yang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0054125.g007", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ATP_S_induces_COX_2_expression_via_a_cPLA_2_AA_signaling_/180736", "title"=>"ATPγS induces COX-2 expression via a cPLA<sub>2</sub>/AA signaling.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 17:18:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/509416"], "description"=>"<p>Cells were pretreated with Edaravone, DPI, or APO for 1 h, and then incubated with ATPγS for (A) 6 h or (B) 2 h. The levels of COX-2 (A) protein and (B) mRNA were analyzed by western blot and real-time PCR, respectively. (A) The media were collected and analyzed for PGE<sub>2</sub> release. Cells were labeled with DCF-DA (10 µM), and then incubated with ATPγS (100 µM) for (C) the indicated time intervals or (D) pretreated with Edaravone (10 µM), DPI (10 µM), or APO (100 µM) for 1 h, and then stimulated with ATPγS (100 µM) for 1 h. The fluorescence intensity (relative DCF fluorescence) was measured and NADPH oxidase activity was determined. Cells were treated with (E) ATPγS for the indicated time intervals or (F) pretreated with Edaravone (10 µM), DPI (10 µM), or APO (100 µM) for 1 h, and then stimulated with ATPγS (100 µM) for 1 h. After incubation, CellROX™ Deep Red Reagent was added at a final concentration of 5 µM to the cells, and then incubated for 30 min at 37°C. Subsequently, medium was removed and the cells were washed thrice with PBS. The resulting fluorescence was measured using a fluorescence microscope. (G) Cells were incubated with ATPγS (100 µM) for the indicated time intervals. The membrane and cytosolic fractions were prepared and analyzed by western blot using an anti-p47<i><sup>phox</sup></i> antibody. (H, I) Cells were transfected with siRNA of scrambled or p47<i><sup>phox</sup></i>, and then incubated with ATPγS for 6 h. The levels of p47<i><sup>phox</sup></i> and COX-2 expression were analyzed by western blot. The media was collected and analyzed for PGE<sub>2</sub> release. Data are expressed as mean±S.E.M. of three independent experiments. *<i>p</i><0.5; <sup>#</sup><i>p</i><0.01, as compared with the cells exposed to ATPγS alone (A, B, D), the cells exposed to vehicle alone (C), or the cells transfected with scrambled siRNA and exposed to ATPγS alone (H, I).</p>", "links"=>[], "tags"=>["induces", "nadph", "oxidase-dependent", "cox-2", "a549"], "article_id"=>179906, "categories"=>["Immunology"], "users"=>["Shin-Ei Cheng", "I-Ta Lee", "Chih-Chung Lin", "Wan-Ling Wu", "Li-Der Hsiao", "Chuen-Mao Yang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0054125.g002", "stats"=>{"downloads"=>4, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ATP_947_S_induces_NADPH_oxidase_dependent_COX_2_expression_in_A549_cells_/179906", "title"=>"ATPγS induces NADPH oxidase-dependent COX-2 expression in A549 cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 17:14:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/510022"], "description"=>"<p>(A) Cells were pretreated with APO (100 µM), DPI (10 µM), Edaravone (10 µM), Gö6983 (10 µM), Gö6976 (10 µM), GF109203X (3 µM), Ro318220 (10 µM), or Rottlerin (10 µM) for 1 h, and then incubated with ATPγS for 1 h. The cytosolic and nuclear fractions were prepared and subjected to Western blot analysis using an anti-phospho-STAT3 or anti-STAT3 antibody. (B) Cells were transfected with siRNAs of scrambled, p47<i><sup>phox</sup></i>, PKCα, PKCι, and PKCμ, and then incubated with ATPγS for 1 h. The cell lysates were subjected to western blot analysis using an anti-phospho-Jak2, anti-phospho-STAT3, or anti-β-actin antibody. Data are expressed as mean±S.E.M. of three independent experiments. <sup>#</sup><i>p</i><0.01, as compared with the cells exposed to scrambled siRNA+ATPγS.</p>", "links"=>[], "tags"=>["regulates", "jak2", "stat3", "activation", "a549"], "article_id"=>180514, "categories"=>["Immunology"], "users"=>["Shin-Ei Cheng", "I-Ta Lee", "Chih-Chung Lin", "Wan-Ling Wu", "Li-Der Hsiao", "Chuen-Mao Yang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0054125.g006", "stats"=>{"downloads"=>2, "page_views"=>104, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ATP_947_S_regulates_PKC_ROS_dependent_Jak2_and_STAT3_activation_in_A549_cells_/180514", "title"=>"ATPγS regulates PKC/ROS-dependent Jak2 and STAT3 activation in A549 cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 17:17:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/509242"], "description"=>"<p>Cells were pretreated with Gö6983, Gö6976, Ro318220, or Rottlerin for 1 h, and then incubated with ATPγS for (A) 6 h or (B) 2 h. The levels of COX-2 (A) protein and (B) mRNA were analyzed by western blot and real-time PCR, respectively. (A) The media were collected and analyzed for PGE<sub>2</sub> release. (C) Cells were treated with ATPγS (100 µM) for the indicated time intervals or PMA (1 µM) for 15 min. The cytosolic and membrane fractions were prepared and analyzed by western blot using an anti-PKCα, anti-PKCι, or anti-PKCμ antibody. β-actin and Gαs were used as a marker protein for cytosolic and membrane fractions, respectively. (D, E) Cells were transfected with siRNA of scrambled, PKCα, PKCι, or PKCμ, and then incubated with ATPγS for 6 h. The expression of PKCα, PKCι, PKCμ, and COX-2 were analyzed by western blot. The media were collected and analyzed for PGE<sub>2</sub> release. Data are expressed as mean±S.E.M. of three independent experiments. *<i>p</i><0.5; <sup>#</sup><i>p</i><0.01, as compared with the cells exposed to ATPγS alone (A, B) or the cells transfected with scrambled siRNA and exposed to ATPγS alone (D, E).</p>", "links"=>[], "tags"=>["regulates", "cox-2", "pkcs", "a549"], "article_id"=>179727, "categories"=>["Immunology"], "users"=>["Shin-Ei Cheng", "I-Ta Lee", "Chih-Chung Lin", "Wan-Ling Wu", "Li-Der Hsiao", "Chuen-Mao Yang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0054125.g001", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ATP_947_S_regulates_COX_2_expression_via_PKCs_in_A549_cells_/179727", "title"=>"ATPγS regulates COX-2 expression via PKCs in A549 cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 17:13:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/509518"], "description"=>"<p>(A) Cells were labeled with DCF-DA (10 µM), pretreated with Gö6983 (10 µM), Gö6976 (10 µM), GF109203X (3 µM), Ro318220 (10 µM), or Rottlerin (10 µM) for 1 h, and then incubated with ATPγS for 1 h. The fluorescence intensity (relative DCF fluorescence) was measured (gray bar). In addition, NADPH oxidase activity was determined (white bar). (B) Cells were pretreated with Gö6983 (10 µM), Gö6976 (10 µM), GF109203X (3 µM), Ro318220 (10 µM), or Rottlerin (10 µM) for 1 h, and then incubated with ATPγS for 1 h. After incubation, ROS generation was determined by using CellROX™ Deep Red Reagent as described in Fig. 2E. (C) Cells were pretreated with Gö6983 (10 µM), Gö6976 (10 µM), GF109203X (3 µM), Ro318220 (10 µM), or Rottlerin (10 µM) for 1 h, and then incubated with ATPγS for 1 h. The membrane and cytosolic fractions were prepared and analyzed by western blot using an anti-p47<i><sup>phox</sup></i> antibody. Data are expressed as mean±S.E.M. of three independent experiments. <sup>#</sup><i>p</i><0.01, as compared with the cells exposed to ATPγS alone.</p>", "links"=>[], "tags"=>["induces", "pkc-dependent", "ros", "a549"], "article_id"=>180017, "categories"=>["Immunology"], "users"=>["Shin-Ei Cheng", "I-Ta Lee", "Chih-Chung Lin", "Wan-Ling Wu", "Li-Der Hsiao", "Chuen-Mao Yang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0054125.g003", "stats"=>{"downloads"=>2, "page_views"=>34, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ATP_947_S_induces_PKC_dependent_ROS_generation_in_A549_cells_/180017", "title"=>"ATPγS induces PKC-dependent ROS generation in A549 cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 17:15:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/509620"], "description"=>"<p>Cells were pretreated with PPADS or suramin for 1 h, and then treated with ATPγS for (A) 6 h or (B) 2 h. The levels of COX-2 (A) protein and (B) mRNA were analyzed by western blot and real-time PCR, respectively. (A) The media were collected and analyzed for PGE<sub>2</sub> release. (C) Cells were labeled with DCF-DA (10 µM), pretreated with PPADS or suramin for 1 h, and then incubated with ATPγS for 1 h. The fluorescence intensity (relative DCF fluorescence) was measured (gray bar). In addition, NADPH oxidase activity was determined (white bar). (D) Cells were pretreated with PPADS or suramin for 1 h, and then incubated with ATPγS for 1 h. After incubation, ROS generation was determined by using CellROX™ Deep Red Reagent as described in Fig. 2E. (E) Cells were pretreated with PPADS (10 µM) or suramin (10 µM) for 1 h, and then incubated with ATPγS for 1 h. The membrane and cytosolic fractions were prepared and analyzed by western blot using an anti-p47<i><sup>phox</sup></i> antibody. (F) Cells were pretreated with PPADS (10 µM) or suramin (10 µM) for 1 h, and then treated with ATPγS for 15 min. The cytosolic and membrane fractions were prepared and analyzed by western blot using an anti-PKCα, anti-PKCι, or anti-PKCμ antibody. Data are expressed as mean±S.E.M. of three independent experiments. <sup>#</sup><i>p</i><0.01, as compared with the cells exposed to ATPγS alone.</p>", "links"=>[], "tags"=>["induces", "p2", "receptor-dependent", "cox-2", "pkc", "ros", "a549"], "article_id"=>180116, "categories"=>["Immunology"], "users"=>["Shin-Ei Cheng", "I-Ta Lee", "Chih-Chung Lin", "Wan-Ling Wu", "Li-Der Hsiao", "Chuen-Mao Yang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0054125.g004", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ATP_947_S_induces_P2_receptor_dependent_COX_2_expression_PKC_translocation_and_ROS_generation_in_A549_cells_/180116", "title"=>"ATPγS induces P2 receptor-dependent COX-2 expression, PKC translocation, and ROS generation in A549 cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 17:15:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/509880"], "description"=>"<p>Cells were pretreated with AG490 or CBE for 1 h, and then incubated with ATPγS for (A) 6 h or (B) 2 h. The levels of COX-2 (A) protein and (B) mRNA were analyzed by western blot and real-time PCR, respectively. (A) The media were collected and analyzed for PGE<sub>2</sub> release. (C) Cells were treated with ATPγS (100 µM) for the indicated time intervals. The cell lysates were analyzed by western blot using an anti-phospho-Jak2, anti-phospho-STAT3, anti-STAT3, or anti-β-actin antibody. Cells were pretreated (D) without or (E) with AG490 (10 µM), CBE (10 µM), PPADS (10 µM), or suramin (10 µM) for 1 h, and then incubated with ATPγS (100 µM) for (D) the indicated time intervals or (E) 60 min. The cytosolic and nuclear fractions were prepared and analyzed by western blot using an anti-phospho-STAT3 or anti-STAT3 antibody. GAPDH and Lamin A were used as a marker protein for cytosolic and nuclear fractions, respectively. (F) Cells were transfected with siRNA of scrambled, Jak2, or STAT3, and then incubated with ATPγS for 6 h. The levels of Jak2, STAT3, and COX-2 expression were analyzed by western blot. The media were collected and analyzed for PGE<sub>2</sub> release. Data are expressed as mean±S.E.M. of three independent experiments. <sup>#</sup><i>p</i><0.01, as compared with the cells exposed to ATPγS alone (A, B), the cells exposed to vehicle alone (C), or the cells transfected with scrambled siRNA and exposed to ATPγS alone (F, G).</p>", "links"=>[], "tags"=>["induces", "cox-2", "a549"], "article_id"=>180374, "categories"=>["Immunology"], "users"=>["Shin-Ei Cheng", "I-Ta Lee", "Chih-Chung Lin", "Wan-Ling Wu", "Li-Der Hsiao", "Chuen-Mao Yang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0054125.g005", "stats"=>{"downloads"=>2, "page_views"=>327, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ATP_947_S_induces_COX_2_expression_via_Jak2_STAT3_in_A549_cells_/180374", "title"=>"ATPγS induces COX-2 expression via Jak2/STAT3 in A549 cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 17:16:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/510360"], "description"=>"<p>ATPγS activates the P2 receptor/PKC/NADPH oxidase pathway to enhance ROS generation, which in turn initiates the activation of Jak2 and STAT3, and ultimately induces COX-2-dependent PGE<sub>2</sub> generation in A549 cells.</p>", "links"=>[], "tags"=>["diagram", "illustrating", "signaling", "pathway", "cox-2", "a549"], "article_id"=>180852, "categories"=>["Immunology"], "users"=>["Shin-Ei Cheng", "I-Ta Lee", "Chih-Chung Lin", "Wan-Ling Wu", "Li-Der Hsiao", "Chuen-Mao Yang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0054125.g008", "stats"=>{"downloads"=>2, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Schematic_diagram_illustrating_the_proposed_signaling_pathway_involved_in_ATP_S_induced_COX_2_expression_and_PGE_2_generation_in_A549_cells_/180852", "title"=>"Schematic diagram illustrating the proposed signaling pathway involved in ATPγS-induced COX-2 expression and PGE<sub>2</sub> generation in A549 cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 17:19:33"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"77", "full-text"=>"80", "pdf"=>"28", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"48", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"61", "full-text"=>"59", "pdf"=>"21", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"53", "full-text"=>"52", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"19", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"43", "full-text"=>"46", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"31", "full-text"=>"31", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"23", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"29", "full-text"=>"23", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"25", "full-text"=>"27", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"25", "full-text"=>"26", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"28", "full-text"=>"35", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"33", "full-text"=>"30", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"18", "full-text"=>"16", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"21", "full-text"=>"19", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"23", "full-text"=>"18", "pdf"=>"9", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"18", "full-text"=>"18", "pdf"=>"11", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"19", "full-text"=>"16", "pdf"=>"11", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"16", "full-text"=>"10", "pdf"=>"9", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"5", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"19", "full-text"=>"19", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"18", "full-text"=>"18", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"18", "full-text"=>"15", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"28", "full-text"=>"31", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"15", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"23", "full-text"=>"28", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"14", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"26", "full-text"=>"24", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"15", "full-text"=>"11", "pdf"=>"1", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"17", "full-text"=>"18", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"14", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"17", "full-text"=>"13", "pdf"=>"10", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"24", "full-text"=>"17", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"15", "full-text"=>"18", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"18", "full-text"=>"16", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"18", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"17", "full-text"=>"20", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"19", "full-text"=>"11", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"21", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"17", "full-text"=>"20", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"19", "full-text"=>"18", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"25", "full-text"=>"19", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"19", "full-text"=>"18", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"21", "full-text"=>"21", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"23", "full-text"=>"14", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"18", "full-text"=>"17", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"17", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"25", "full-text"=>"11", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"22", "full-text"=>"13", "pdf"=>"9", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"32", "full-text"=>"12", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"19", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"71", "full-text"=>"52", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"25", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[266, 468, 593, 703, 804, 903, 993, 1084, 1171, 1256, 1339, 1422, 1492]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[272, 472, 600, 713, 815, 911, 1004, 1094, 1185, 1273, 1358, 1441]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[284, 491, 620, 738, 843, 945, 1043, 1137, 1225, 1315, 1400, 1479, 1555]}, {"subject_area"=>"/Biology and life sciences/Immunology", "average_usage"=>[266, 466, 591, 701, 799, 887, 982, 1067, 1155, 1237, 1317, 1395, 1458]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[264, 460, 584, 692, 794, 887, 978, 1067, 1154, 1241, 1328, 1408, 1474]}, {"subject_area"=>"/Medicine and health sciences/Immunology", "average_usage"=>[262, 463, 587, 693, 790, 882, 965, 1051, 1135, 1218, 1300, 1376, 1438]}, {"subject_area"=>"/Medicine and health sciences/Pain management", "average_usage"=>[259, 460, 588, 720, 834, 951, 1056, 1139, 1222, 1315, 1391, 1497, 1570]}, {"subject_area"=>"/Medicine and health sciences/Pharmacology", "average_usage"=>[265, 471, 604, 723, 827, 926, 1022, 1113, 1210, 1290, 1377, 1454, 1532]}]}
Loading … Spinner
There are currently no alerts