High Glucose Induced Alteration of SIRTs in Endothelial Cells Causes Rapid Aging in a p300 and FOXO Regulated Pathway
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"High Glucose Induced Alteration of SIRTs in Endothelial Cells Causes Rapid Aging in a p300 and FOXO Regulated Pathway", "type"=>"journal", "authors"=>[{"first_name"=>"Rokhsana", "last_name"=>"Mortuza", "scopus_author_id"=>"55559431600"}, {"first_name"=>"Shali", "last_name"=>"Chen", "scopus_author_id"=>"7410254319"}, {"first_name"=>"Biao", "last_name"=>"Feng", "scopus_author_id"=>"35279604300"}, {"first_name"=>"Subhrojit", "last_name"=>"Sen", "scopus_author_id"=>"7403696945"}, {"first_name"=>"Subrata", "last_name"=>"Chakrabarti", "scopus_author_id"=>"57200654544"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"368133780", "sgr"=>"84872448285", "pmid"=>"23342163", "scopus"=>"2-s2.0-84872448285", "isbn"=>"10.1371/journal.pone.0054514", "doi"=>"10.1371/journal.pone.0054514", "issn"=>"19326203"}, "id"=>"744f492f-b2ea-39fa-8189-13cf1cdea819", "abstract"=>"In diabetes, some of the cellular changes are similar to aging. We hypothesized that hyperglycemia accelerates aging-like changes in the endothelial cells (ECs) and tissues leading to structural and functional damage. We investigated glucose-induced aging in 3 types of ECs using senescence associated β-gal (SA β-gal) staining and cell morphology. Alterations of sirtuins (SIRTs) and their downstream mediator FOXO and oxidative stress were investigated. Relationship of such alteration with histone acetylase (HAT) p300 was examined. Similar examinations were performed in tissues of diabetic animals. ECs in high glucose (HG) showed evidence of early senescence as demonstrated by increased SA β-gal positivity and reduced replicative capacities. These alterations were pronounced in microvascular ECs. They developed an irregular and hypertrophic phenotype. Such changes were associated with decreased SIRT (1-7) mRNA expressions. We also found that p300 and SIRT1 regulate each other in such process, as silencing one led to increase of the others' expression. Furthermore, HG caused reduction in FOXO1's DNA binding ability and antioxidant target gene expressions. Chemically induced increased SIRT1 activity and p300 knockdown corrected these abnormalities slowing aging-like changes. Diabetic animals showed increased cellular senescence in renal glomerulus and retinal blood vessels along with reduced SIRT1 mRNA expressions in these tissues. Data from this study demonstrated that hyperglycemia accelerates aging-like process in the vascular ECs and such process is mediated via downregulation of SIRT1, causing reduction of mitochondrial antioxidant enzyme in a p300 and FOXO1 mediated pathway.", "link"=>"http://www.mendeley.com/research/high-glucose-induced-alteration-sirts-endothelial-cells-causes-rapid-aging-p300-foxo-regulated-pathw", "reader_count"=>68, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>4, "Researcher"=>15, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>18, "Student > Postgraduate"=>4, "Student > Master"=>11, "Other"=>1, "Student > Bachelor"=>4, "Lecturer"=>2, "Professor"=>5}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>4, "Researcher"=>15, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>18, "Student > Postgraduate"=>4, "Student > Master"=>11, "Other"=>1, "Student > Bachelor"=>4, "Lecturer"=>2, "Professor"=>5}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>12, "Medicine and Dentistry"=>18, "Agricultural and Biological Sciences"=>32, "Neuroscience"=>1, "Arts and Humanities"=>1, "Physics and Astronomy"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>18}, "Neuroscience"=>{"Neuroscience"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>32}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>12}, "Unspecified"=>{"Unspecified"=>1}, "Arts and Humanities"=>{"Arts and Humanities"=>1}}, "reader_count_by_country"=>{"Sweden"=>1, "United States"=>2, "Finland"=>1, "Poland"=>1, "United Kingdom"=>1, "Italy"=>1}, "group_count"=>1}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84872448285"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84872448285?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84872448285&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84872448285&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84872448285", "dc:identifier"=>"SCOPUS_ID:84872448285", "eid"=>"2-s2.0-84872448285", "dc:title"=>"High Glucose Induced Alteration of SIRTs in Endothelial Cells Causes Rapid Aging in a p300 and FOXO Regulated Pathway", "dc:creator"=>"Mortuza R.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"8", "prism:issueIdentifier"=>"1", "prism:pageRange"=>nil, "prism:coverDate"=>"2013-01-16", "prism:coverDisplayDate"=>"16 January 2013", "prism:doi"=>"10.1371/journal.pone.0054514", "citedby-count"=>"85", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Western University", "affiliation-city"=>"London", "affiliation-country"=>"Canada"}], "pubmed-id"=>"23342163", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e54514", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0054514", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Twitter

Counter

  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"95", "xml_views"=>"2", "html_views"=>"419"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"67", "xml_views"=>"3", "html_views"=>"182"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"54", "xml_views"=>"1", "html_views"=>"161"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"44", "xml_views"=>"3", "html_views"=>"137"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"41", "xml_views"=>"1", "html_views"=>"122"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"30", "xml_views"=>"0", "html_views"=>"91"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"31", "xml_views"=>"0", "html_views"=>"117"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"66", "xml_views"=>"0", "html_views"=>"132"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"40", "xml_views"=>"0", "html_views"=>"111"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"39", "xml_views"=>"2", "html_views"=>"156"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"55", "xml_views"=>"0", "html_views"=>"222"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"48", "xml_views"=>"0", "html_views"=>"177"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"32", "xml_views"=>"0", "html_views"=>"85"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"32", "xml_views"=>"2", "html_views"=>"123"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"41", "xml_views"=>"1", "html_views"=>"139"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"99"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"88"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"75"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"28", "xml_views"=>"2", "html_views"=>"65"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"29", "xml_views"=>"1", "html_views"=>"113"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"25", "xml_views"=>"4", "html_views"=>"98"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"39", "xml_views"=>"1", "html_views"=>"104"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"115"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"23", "xml_views"=>"1", "html_views"=>"86"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"74"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"48"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"52"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"79"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"167"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"123"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"180"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"148"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"90"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"106"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"86"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"88"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"100"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"67"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"19", "xml_views"=>"1", "html_views"=>"81"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"25", "xml_views"=>"1", "html_views"=>"94"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"84"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"18", "xml_views"=>"1", "html_views"=>"65"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"22", "xml_views"=>"1", "html_views"=>"86"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"166"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"60"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"16", "xml_views"=>"2", "html_views"=>"46"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"49"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"43"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"25"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"3", "html_views"=>"15"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"7"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/276665", "https://ndownloader.figshare.com/files/276767", "https://ndownloader.figshare.com/files/276809"], "description"=>"<div><p>In diabetes, some of the cellular changes are similar to aging. We hypothesized that hyperglycemia accelerates aging-like changes in the endothelial cells (ECs) and tissues leading to structural and functional damage. We investigated glucose-induced aging in 3 types of ECs using senescence associated β-gal (SA β-gal) staining and cell morphology. Alterations of sirtuins (SIRTs) and their downstream mediator FOXO and oxidative stress were investigated. Relationship of such alteration with histone acetylase (HAT) p300 was examined. Similar examinations were performed in tissues of diabetic animals. ECs in high glucose (HG) showed evidence of early senescence as demonstrated by increased SA β-gal positivity and reduced replicative capacities. These alterations were pronounced in microvascular ECs. They developed an irregular and hypertrophic phenotype. Such changes were associated with decreased SIRT (1–7) mRNA expressions. We also found that p300 and SIRT1 regulate each other in such process, as silencing one led to increase of the others’ expression. Furthermore, HG caused reduction in FOXO1’s DNA binding ability and antioxidant target gene expressions. Chemically induced increased SIRT1 activity and p300 knockdown corrected these abnormalities slowing aging-like changes. Diabetic animals showed increased cellular senescence in renal glomerulus and retinal blood vessels along with reduced SIRT1 mRNA expressions in these tissues. Data from this study demonstrated that hyperglycemia accelerates aging-like process in the vascular ECs and such process is mediated via downregulation of SIRT1, causing reduction of mitochondrial antioxidant enzyme in a p300 and FOXO1 mediated pathway.</p> </div>", "links"=>[], "tags"=>["glucose", "induced", "alteration", "sirts", "endothelial", "cells", "causes", "aging", "p300", "foxo", "regulated", "pathway"], "article_id"=>114529, "categories"=>["Chemistry", "Molecular Biology", "Physiology", "Biological Sciences", "Cell Biology", "Genetics"], "users"=>["Rokhsana Mortuza", "Shali Chen", "Biao Feng", "Subhrojit Sen", "Subrata Chakrabarti"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0054514.s001", "https://dx.doi.org/10.1371/journal.pone.0054514.s002", "https://dx.doi.org/10.1371/journal.pone.0054514.s003"], "stats"=>{"downloads"=>19, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/High_Glucose_Induced_Alteration_of_SIRTs_in_Endothelial_Cells_Causes_Rapid_Aging_in_a_p300_and_FOXO_Regulated_Pathway__/114529", "title"=>"High Glucose Induced Alteration of SIRTs in Endothelial Cells Causes Rapid Aging in a p300 and FOXO Regulated Pathway", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-01-16 01:15:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/507027"], "description"=>"<p>(A) SIRT (1–7) mRNA expressions are decreased in large vessel endothelial cells (HUVEC) with increasing passages and with HG it is escalated (analyzed by quantitative Real Time RT-PCR). mRNA levels are expressed as a ratio to β-actin and normalized to baseline controls, NG P0 (before treatment began). [*p<0.05 compared to NGP0; ‡p<0.05 compared to respective NG passages for HG cultured cells]. (B) SIRT1 enzyme activity was reduced in HG in HUVEC (P11, data normalized to NG). [*p<0.05 compared to NGP11]. (NG = 5 mM; HG = 25 mM glucose, P = passage number).</p>", "links"=>[], "tags"=>["mrna", "reductions", "augmented", "hg"], "article_id"=>177541, "categories"=>["Chemistry", "Molecular Biology", "Physiology", "Biological Sciences", "Cell Biology", "Genetics"], "users"=>["Rokhsana Mortuza", "Shali Chen", "Biao Feng", "Subhrojit Sen", "Subrata Chakrabarti"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0054514.g005", "stats"=>{"downloads"=>3, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_SIRT_1_8211_7_mRNA_reductions_with_increasing_passage_number_is_augmented_with_HG_treatment_in_HUVECs_/177541", "title"=>"SIRT (1–7) mRNA reductions with increasing passage number is augmented with HG treatment in HUVECs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-16 02:05:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/507466"], "description"=>"<p>(A) Quantitative Real Time RT-PCR analysis showed HG upregulated p300 mRNA and such effects are augmented by SIRT1 knockdown with siRNA. (B) HG induced downregulation of SIRT1 mRNA expression was corrected with p300 knockdown with siRNA. mRNA levels are expressed as ratio of 18s normalized to NG. (C) p300 siRNA reduced total ROS levels in HG by increasing (D) FOXO1’s DNA binding activity subsequently increasing (E) the MnSOD levels. (F, G) Microscopic photographs and quantitative analysis showed glucose induced increased SA β-gal positivity is effectively prevented by p300 siRNA. Data normalized to NG. (NG = 5 mM; HG = 25 mM D-glucose, OSM = 25 mM L-glucose, P1 =  passage1). [*p<0.05 and **p<0.01 compared to NG; ‡P<0.05 and ‡‡p<0.01 compared to HG. Scale bar represent 100 µm for all micrographs. siRNA1 and siRNA2 represent separate experiments using two different siRNAs].</p>", "links"=>[], "tags"=>["p300", "microvascular", "endothelial"], "article_id"=>177958, "categories"=>["Chemistry", "Molecular Biology", "Physiology", "Biological Sciences", "Cell Biology", "Genetics"], "users"=>["Rokhsana Mortuza", "Shali Chen", "Biao Feng", "Subhrojit Sen", "Subrata Chakrabarti"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0054514.g008", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_SIRT1_and_p300_regulate_each_other_in_microvascular_endothelial_cells_/177958", "title"=>"SIRT1 and p300 regulate each other in microvascular endothelial cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-16 02:12:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/506777"], "description"=>"<p>(A) HMEC, BREC & HUVEC showed reduction of TERT (telomerase reverse transcriptase) mRNA in HG treated cells. mRNA levels are expressed as a ratio to 18s and normalized to NG P1. (B) Total ROS levels in HMEC, BREC & HUVEC showed significant increase with HG and aging. MnSOD levels in all cells at end passages (P5 in HMEC, P8 in BREC and P11 in HUVEC) showed significant reduction with HG treatment (C). (NG = 5 mM; HG = 25 mM glucose, P1 =  passage 1). Data normalized to NG (P1) treatments of the respective ECs. [*p<0.05 compared to NGP1; ‡p<0.05 compared to NGP5 for HMEC, NGP8 for BREC and NGP11 for HUVEC].</p>", "links"=>[], "tags"=>["oxidative", "reduced", "tert", "mrna", "observed", "endothelial", "cells"], "article_id"=>177290, "categories"=>["Chemistry", "Molecular Biology", "Physiology", "Biological Sciences", "Cell Biology", "Genetics"], "users"=>["Rokhsana Mortuza", "Shali Chen", "Biao Feng", "Subhrojit Sen", "Subrata Chakrabarti"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0054514.g003", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Increased_oxidative_stress_and_reduced_TERT_mRNA_expression_observed_in_endothelial_cells_with_HG_/177290", "title"=>"Increased oxidative stress and reduced TERT mRNA expression observed in endothelial cells with HG.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-16 02:01:30"}
  • {"files"=>["https://ndownloader.figshare.com/files/507605"], "description"=>"<p>(A) SA β-gal staining of kidney tissue of STZ induced diabetic mice at 2 and 4 months showing increased positivity with uncontrolled diabetes. Arrow indicates β-gal positive cells. (B) Quantification of β-gal staining of the kidney tissue. Data presented as β-gal positivity/view (n = 10 image). (C) Shows reduction of MnSOD levels in kidney tissues of the diabetic animals following 2 and 4 months. (D) mRNA analysis confirmed downregulation of SIRT1 expressions in the kidney tissues of these animals. mRNA levels are expressed as a ratio to 18 s normalized to controls. (E) FOXO1 DNA binding and (G) SIRT1 enzyme activities are reduced in the kidney tissues in diabetes. (F) Western blot analysis showed increased p300 protein levels in the kidneys in diabetes. Data normalized to controls. [*p<0.05 compared to control animals. Scale bar represents 100 µm for all micrographs].</p>", "links"=>[], "tags"=>["causes", "accelerated", "aging", "mice"], "article_id"=>178095, "categories"=>["Chemistry", "Molecular Biology", "Physiology", "Biological Sciences", "Cell Biology", "Genetics"], "users"=>["Rokhsana Mortuza", "Shali Chen", "Biao Feng", "Subhrojit Sen", "Subrata Chakrabarti"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0054514.g009", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Diabetes_causes_accelerated_aging_in_mice_kidney_/178095", "title"=>"Diabetes causes accelerated aging in mice kidney.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-16 02:14:55"}
  • {"files"=>["https://ndownloader.figshare.com/files/506654"], "description"=>"<p>(A) SA-βgal staining showed increased β-gal positivity (blue cells as indicated by arrow) with HG treatment compared to NG and osmotic control groups. Aging signs appeared later with HG in HUVEC. [Scale bar represent 100 µm for all micrographs]. (B) Quantification of β-gal positivity shows increased percentage of aged cells in HG treated group. (C) Morphometric cell area analysis showed significant increase in cell size with HG treatment. Cell growth was slow with HG treatment compared to controls as seen from (D) days needed to be confluent in HG (90%, at which stage cells were subcultured to next passage), (E) cell count in HG (attached cells per microscopic field at 20× objective, n = 10 image) and reduced (F) intracellular LDH activity in HG. (NG = normal glucose, 5 mM; HG = high glucose, 25 mM D-glucose; OSM = osmotic control, 25 mM L-glucose; P = passage number). [*p<0.05, **p<0.01 compared to NGP1; ‡p<0.05, ‡‡p<0.01 compared to respective NG passages for HG cultured cells].</p>", "links"=>[], "tags"=>["aging", "endothelial", "cells", "hg"], "article_id"=>177168, "categories"=>["Chemistry", "Molecular Biology", "Physiology", "Biological Sciences", "Cell Biology", "Genetics"], "users"=>["Rokhsana Mortuza", "Shali Chen", "Biao Feng", "Subhrojit Sen", "Subrata Chakrabarti"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0054514.g002", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Accelerated_aging_in_large_vessel_endothelial_cells_HUVEC_with_HG_is_delayed_/177168", "title"=>"Accelerated aging in large vessel endothelial cells (HUVEC) with HG is delayed.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-16 01:59:28"}
  • {"files"=>["https://ndownloader.figshare.com/files/506467"], "description"=>"<p>(A) SA-βgal staining showed increased β-gal positivity or senescent cells with HG treatment compared to the NG treated cells, with the increasing passage number. Arrow indicates β-gal positive (blue) cells. [Scale bar represent 100 µm for all micrographs]. (B) Quantification of β-gal positivity (n = 10 image per sample). Signs of cellular aging started to appear from passage 1 in HG. (C) Morphometric cell area analysis showed significant increase in cell size with HG treatment. Cell growth was slow with HG treatment compared to controls as seen from (D) days needed to be confluent in HG (90%, at which stage cells were subcultured to next passage), (E) cell count in HG (attached cells per microscopic field at 20× objective, n = 10 image) and (F) reduced intracellular LDH activity in HG. (NG = normal glucose, 5 mM; HG = high glucose, 25 mM D-glucose; OSM = osmotic control, 25 Mm L-glucose; P = passage number). [*p<0.05 compared to NGP1; ‡p<0.05, ‡‡p<0.01 compared to respective NG passages for HG cultured cells].</p>", "links"=>[], "tags"=>["signs", "microvascular", "endothelial", "cells", "hg", "starts"], "article_id"=>176978, "categories"=>["Chemistry", "Molecular Biology", "Physiology", "Biological Sciences", "Cell Biology", "Genetics"], "users"=>["Rokhsana Mortuza", "Shali Chen", "Biao Feng", "Subhrojit Sen", "Subrata Chakrabarti"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0054514.g001", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Aging_signs_in_microvascular_endothelial_cells_HMEC_with_HG_starts_as_early_as_passage_1_/176978", "title"=>"Aging signs in microvascular endothelial cells (HMEC) with HG starts as early as passage 1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-16 01:56:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/507732"], "description"=>"<p>(A) MnSOD levels were reduced in kidneys of <i>db/db</i> mice following 2 and 4 months of diabetes indicating increased oxidative stress. (B, C) SIRT1 mRNA expression and FOXO1 activity in kidneys of <i>db/db</i> mice were reduced compared to controls. (D) SA β-gal staining of type 1 diabetic mice retina showed increased positivity in retinal blood vessels following 2 months of diabetes. [Scale bar represent 50 µm for all micrographs]. Arrow indicates retinal blood vessels. (E) Such changes were associated with reduced MnSOD level in these tissues. (F, G) Shows reduction of SIRT1 mRNA expressions in retinal tissues of both type 1 and 2 diabetic mice. mRNA levels are expressed as a ratio to 18s normalized to controls. [*p<0.05 compared to control animals].</p>", "links"=>[], "tags"=>["changes", "kidneys", "retinas", "diabetic"], "article_id"=>178229, "categories"=>["Chemistry", "Molecular Biology", "Physiology", "Biological Sciences", "Cell Biology", "Genetics"], "users"=>["Rokhsana Mortuza", "Shali Chen", "Biao Feng", "Subhrojit Sen", "Subrata Chakrabarti"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0054514.g010", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Oxidative_stress_and_associated_changes_are_present_in_the_kidneys_and_retinas_of_diabetic_animals_/178229", "title"=>"Oxidative stress and associated changes are present in the kidneys and retinas of diabetic animals.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-16 02:17:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/507328"], "description"=>"<p>(A) Early senescence was induced with FOXO1 inhibitor (AS1842856) in NG, and the rescue of senescence with resveratrol in HG disappeared with FOXO1 inhibitor, as evidenced by SA-β gal stain (HMECP1). (B) In NG, AS1842856 reduced MnSOD and increased (C) ROS levels; it also hindered the preventative effect of resveratrol in HG regarding these parameters. [*p<0.05, **p<0.01 compared to NG; ‡p<0.05 compared to HG; †p<0.05 compared to HG+Resveratrol]. (D) SIRT1 knockdown with siRNA induced senescence in NG as seen using SA-β gal stain (HMECP1). (E) Such knockdown also increased ROS levels. siRNA1 and siRNA2 represent separate experiments using two different siRNAs. (F) Glucose induced increased SA β-gal positivity was reduced with resveratrol. However SIRT1 siRNA transfection prevented such effect. (G) Analysis of SIRT1 mRNA level showed such effect of resveratrol is mediated through SIRT1. mRNA levels are expressed as a ratio to 18s normalized to controls. (H) Glucose induced increased ROS production was prevented by resveratrol. However such preventative effects were partially lost with SIRT1 siRNA transfection. [*p<0.05, **p<0.01 compared to NG; ‡p<0.05 compared to HG; †p<0.05 compared to HG+Res.+scramble]. (NG = 5 mM; HG = 25 mM glucose, P1 =  passage 1). [Scale bar represent 100 µm for all micrographs].</p>", "links"=>[], "tags"=>["knockdown", "foxo1", "inhibition", "ng", "induces", "signs", "aging", "mimicking", "hg"], "article_id"=>177814, "categories"=>["Chemistry", "Molecular Biology", "Physiology", "Biological Sciences", "Cell Biology", "Genetics"], "users"=>["Rokhsana Mortuza", "Shali Chen", "Biao Feng", "Subhrojit Sen", "Subrata Chakrabarti"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0054514.g007", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_SIRT1_knockdown_or_FOXO1_inhibition_in_NG_induces_signs_of_early_aging_mimicking_the_HG_treatment_/177814", "title"=>"SIRT1 knockdown or FOXO1 inhibition in NG induces signs of early aging mimicking the HG treatment.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-16 02:10:14"}
  • {"files"=>["https://ndownloader.figshare.com/files/507150"], "description"=>"<p>(A) SA β-gal staining of HMEC (P1) with resveratrol and BML278 treatment. β-gal positivity was reduced with SIRT1 activators in HG treated cells compared to controls. [Scale bar represent 100 µm for all micrographs]. (B) Quantification of β-gal positivity. (C) SIRT1 activators significantly increased the enzyme’s activity and reduced (D) total ROS levels in HG. (E) FOXO1 DNA binding activity was reduced in the nuclear fractions of HMEC with SIRT1 activators along with increased (F) MnSOD levels. (G) Western blot analysis of acetylated FOXO1 shows HG induced increase in Ac-FOXO1 level was corrected with SIRT1 activators. HG caused (H) nonsignificant change in TERT mRNA expression, however (I) telomerase activity was significantly reduced in the ECs and such reductions were prevented with SIRT1 activators. mRNA levels are expressed as a ratio to β-actin. All data were normalized to NG. Osmotic controls with L-glucose had no effect. [*p<0.05, **p<0.01 compared to NG; ‡p<0.05 compared to HG].</p>", "links"=>[], "tags"=>["induced", "activation", "sirt1", "oxidative", "hg", "treated", "endothelial"], "article_id"=>177660, "categories"=>["Chemistry", "Molecular Biology", "Physiology", "Biological Sciences", "Cell Biology", "Genetics"], "users"=>["Rokhsana Mortuza", "Shali Chen", "Biao Feng", "Subhrojit Sen", "Subrata Chakrabarti"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0054514.g006", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Chemically_induced_activation_of_SIRT1_reduces_oxidative_stress_in_HG_treated_endothelial_cells_/177660", "title"=>"Chemically induced activation of SIRT1 reduces oxidative stress in HG treated endothelial cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-16 02:07:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/506922"], "description"=>"<p>(A) Quantitative Real Time RT-PCR of SIRTs in HMEC showed significant reduction in the SIRT mRNA levels in HG treated cells. mRNA levels are expressed as a ratio to β-actin normalized to baseline controls, NG P0 (P0 =  before start of treatment). [*p<0.05 compared to NGP0; ‡p<0.05 compared to respective NG passages for HG cultured cells]. (B) SIRT1 enzyme activity was reduced in HG in these endothelial cells (P5, data normalized to NG). (NG = 5 mM; HG = 25 mM glucose; P = passage number). [*p<0.05 compared to NGP5].</p>", "links"=>[], "tags"=>["mrna", "hg", "microvascular", "endothelial", "cells", "parallels", "accelerated"], "article_id"=>177439, "categories"=>["Chemistry", "Molecular Biology", "Physiology", "Biological Sciences", "Cell Biology", "Genetics"], "users"=>["Rokhsana Mortuza", "Shali Chen", "Biao Feng", "Subhrojit Sen", "Subrata Chakrabarti"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0054514.g004", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_SIRT_1_8211_7_mRNA_reduction_in_HG_in_microvascular_endothelial_cells_HMECs_parallels_the_accelerated_aging_/177439", "title"=>"SIRT (1–7) mRNA reduction in HG in microvascular endothelial cells (HMECs) parallels the accelerated aging.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-16 02:03:59"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"34", "full-text"=>"37", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"103", "full-text"=>"95", "pdf"=>"45", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"87", "full-text"=>"95", "pdf"=>"44", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"25", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"65", "full-text"=>"63", "pdf"=>"30", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"47", "full-text"=>"60", "pdf"=>"25", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"47", "full-text"=>"38", "pdf"=>"37", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"38", "full-text"=>"35", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"46", "full-text"=>"48", "pdf"=>"31", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"21", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"48", "full-text"=>"45", "pdf"=>"60", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"19", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"51", "full-text"=>"57", "pdf"=>"51", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"31", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"43", "full-text"=>"44", "pdf"=>"33", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"37", "full-text"=>"36", "pdf"=>"26", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"43", "full-text"=>"42", "pdf"=>"31", "abstract"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"58", "full-text"=>"59", "pdf"=>"31", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"37", "full-text"=>"36", "pdf"=>"31", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"28", "full-text"=>"29", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"25", "full-text"=>"26", "pdf"=>"19", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"33", "full-text"=>"36", "pdf"=>"23", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"23", "full-text"=>"15", "pdf"=>"22", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"30", "full-text"=>"32", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"22", "full-text"=>"16", "pdf"=>"16", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"23", "full-text"=>"21", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"35", "full-text"=>"40", "pdf"=>"24", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"26", "full-text"=>"23", "pdf"=>"20", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"18", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"26", "full-text"=>"20", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"32", "full-text"=>"30", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"22", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"23", "full-text"=>"23", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"39", "full-text"=>"41", "pdf"=>"22", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"23", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"27", "full-text"=>"35", "pdf"=>"22", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"38", "full-text"=>"32", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"26", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"25", "full-text"=>"25", "pdf"=>"19", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"21", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"25", "full-text"=>"24", "pdf"=>"16", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"23", "full-text"=>"24", "pdf"=>"18", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"43", "full-text"=>"32", "pdf"=>"36", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"40", "full-text"=>"39", "pdf"=>"20", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"28", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"27", "full-text"=>"29", "pdf"=>"23", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"29", "full-text"=>"27", "pdf"=>"16", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"18", "full-text"=>"13", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"32", "full-text"=>"33", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"19", "full-text"=>"19", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"30", "full-text"=>"36", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"26", "full-text"=>"31", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"21", "full-text"=>"23", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"30", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"21", "full-text"=>"22", "pdf"=>"16", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"23", "full-text"=>"21", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"25", "full-text"=>"29", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"25", "full-text"=>"26", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"25", "full-text"=>"26", "pdf"=>"16", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"26", "full-text"=>"25", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"24", "full-text"=>"30", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"16", "full-text"=>"18", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"26", "full-text"=>"26", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"16", "full-text"=>"18", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"19", "full-text"=>"18", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"27", "full-text"=>"27", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"22", "full-text"=>"23", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"20", "full-text"=>"18", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"14", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"18", "full-text"=>"22", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"21", "full-text"=>"22", "pdf"=>"10", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"16", "full-text"=>"19", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"19", "full-text"=>"18", "pdf"=>"9", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"24", "full-text"=>"18", "pdf"=>"9", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"17", "full-text"=>"21", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"22", "full-text"=>"25", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"20", "full-text"=>"21", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"16", "full-text"=>"12", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"16", "full-text"=>"19", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"21", "full-text"=>"25", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Anatomy", "average_usage"=>[248, 440, 562, 666, 765, 856, 944, 1028, 1112, 1198, 1282, 1362, 1430]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[272, 472, 600, 713, 815, 911, 1004, 1094, 1185, 1273, 1358, 1441]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[284, 491, 620, 738, 843, 945, 1043, 1137, 1225, 1315, 1400, 1479, 1555]}, {"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[272, 466, 589, 702, 806, 903, 995, 1086, 1176, 1258, 1347, 1422, 1493]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[264, 460, 584, 692, 794, 887, 978, 1067, 1154, 1241, 1328, 1408, 1474]}, {"subject_area"=>"/Medicine and health sciences/Anatomy", "average_usage"=>[248, 441, 564, 668, 769, 859, 948, 1034, 1117, 1202, 1290, 1368, 1437]}, {"subject_area"=>"/Medicine and health sciences/Anatomy and physiology", "average_usage"=>[253, 430]}, {"subject_area"=>"/Medicine and health sciences/Metabolic disorders", "average_usage"=>[254, 466, 587, 690, 790, 885, 983, 1063, 1139, 1216, 1297, 1373, 1442]}, {"subject_area"=>"/Physical sciences", "average_usage"=>[254, 431, 547, 651, 748, 842, 932, 1017, 1098, 1178, 1259, 1336, 1404]}, {"subject_area"=>"/Physical sciences/Chemistry", "average_usage"=>[247, 429, 544, 647, 747, 842, 929, 1012, 1099, 1179, 1263, 1339, 1409]}]}
Loading … Spinner
There are currently no alerts