Tobacco Mosaic Virus in the Lungs of Mice following Intra-Tracheal Inoculation
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{"title"=>"Tobacco Mosaic Virus in the Lungs of Mice following Intra-Tracheal Inoculation", "type"=>"journal", "authors"=>[{"first_name"=>"Fanny", "last_name"=>"Balique", "scopus_author_id"=>"36636707700"}, {"first_name"=>"Philippe", "last_name"=>"Colson", "scopus_author_id"=>"7102090499"}, {"first_name"=>"Abdoulaye Oury", "last_name"=>"Barry", "scopus_author_id"=>"57196976507"}, {"first_name"=>"Claude", "last_name"=>"Nappez", "scopus_author_id"=>"17342406700"}, {"first_name"=>"Audrey", "last_name"=>"Ferretti", "scopus_author_id"=>"55317299700"}, {"first_name"=>"Khatoun Al", "last_name"=>"Moussawi", "scopus_author_id"=>"57193956222"}, {"first_name"=>"Tatsiana", "last_name"=>"Ngounga", "scopus_author_id"=>"55596113500"}, {"first_name"=>"Hubert", "last_name"=>"Lepidi", "scopus_author_id"=>"7003303905"}, {"first_name"=>"Eric", "last_name"=>"Ghigo", "scopus_author_id"=>"7103350754"}, {"first_name"=>"Jean Louis", "last_name"=>"Mege", "scopus_author_id"=>"7004286208"}, {"first_name"=>"Hervé", "last_name"=>"Lecoq", "scopus_author_id"=>"7004594454"}, {"first_name"=>"Didier", "last_name"=>"Raoult", "scopus_author_id"=>"55144875700"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84873854916", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pui"=>"368227263", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0054993", "scopus"=>"2-s2.0-84873854916", "pmid"=>"23383021"}, "id"=>"39b4e268-5939-3bc5-8fce-ea4a23a03472", "abstract"=>"Plant viruses are generally considered incapable of infecting vertebrates. Accordingly, they are not considered harmful for humans. However, a few studies questioned the certainty of this paradigm. Tobacco mosaic virus (TMV) RNA has been detected in human samples and TMV RNA translation has been described in animal cells. We sought to determine if TMV is detectable, persists, and remains viable in the lung tissues of mice following intratracheal inoculation, and we attempted to inoculate mouse macrophages with TMV. In the animal model, mice were intratracheally inoculated with 10(11) viral particles and were sacrificed at different time points. The virus was detected in the mouse lungs using immunohistochemistry, electron microscopy, real-time RT-PCR and sequencing, and its viability was studied with an infectivity assay on plants. In the cellular model, the culture medium of murine bone marrow derived macrophages (BMDM) was inoculated with different concentrations of TMV, and the virus was detected with real-time RT-PCR and immunofluorescence. In addition, anti-TMV antibodies were detected in mouse sera with ELISA. We showed that infectious TMV could enter and persist in mouse lungs via the intratracheal route. Over 14 days, the TMV RNA level decreased by 5 log(10) copies/ml in the mouse lungs and by 3.5 log(10) in macrophages recovered from bronchoalveolar lavage. TMV was localized to lung tissue, and its infectivity was observed on plants until 3 days after inoculation. In addition, anti-TMV antibody seroconversions were observed in the sera from mice 7 days after inoculation. In the cellular model, we observed that TMV persisted over 15 days after inoculation and it was visualized in the cytoplasm of the BMDM. This work shows that a plant virus, Tobacco mosaic virus, could persist and enter in cells in mammals, which raises questions about the potential interactions between TMV and human hosts.", "link"=>"http://www.mendeley.com/research/tobacco-mosaic-virus-lungs-mice-following-intratracheal-inoculation-2", "reader_count"=>14, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Researcher"=>5, "Student > Ph. D. Student"=>3, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Researcher"=>5, "Student > Ph. D. Student"=>3, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>4, "Veterinary Science and Veterinary Medicine"=>1, "Chemistry"=>1, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Chemistry"=>{"Chemistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>4}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>3}, "Environmental Science"=>{"Environmental Science"=>1}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/498745"], "description"=>"<p>Note the absence of inflammation in the lungs of control mice, whereas inflammatory infiltrates in interalveolar walls composed in part by macrophages were observed in lungs from TMV-inoculated mice. Hematoxylin-eosin staining was used. Magnification, 200X (A and B) and 400X (C and D). Arrows indicate the inter-alveolar walls inflammation.</p>", "links"=>[], "tags"=>["sections", "water-inoculated", "tmv-inoculated", "intratracheal"], "article_id"=>169239, "categories"=>["Virology", "Microbiology", "Plant Biology"], "users"=>["Fanny Balique", "Philippe Colson", "Abdoulaye Oury Barry", "Claude Nappez", "Audrey Ferretti", "Khatoun Al Moussawi", "Tatsiana Ngounga", "Hubert Lepidi", "Eric Ghigo", "Jean-Louis Mege", "Hervé Lecoq", "Didier Raoult"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0054993.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Lung_sections_from_water_inoculated_mouse_A_and_C_and_TMV_inoculated_mouse_at_day_3_B_and_D_after_intratracheal_inoculation_/169239", "title"=>"Lung sections from water-inoculated mouse (A and C), and TMV-inoculated mouse at day 3 (B and D) after intratracheal inoculation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 16:15:59"}
  • {"files"=>["https://ndownloader.figshare.com/files/498951"], "description"=>"<p>No immunodetection was observed in lung from a water-inoculated mouse (A), whereas cytoplasmically immunopositive cells located in inflammatory infiltrates present in interalveolar walls, most likely macrophages, were detected at day 1 (B) and day 3 (C) after intratracheal inoculation. Polyclonal rabbit anti-TMV antibody was used at a dilution of 1∶500, with hemaxylin counterstain. Magnification, 400X. Arrows show macrophages positive for TMV antigen detection.</p>", "links"=>[], "tags"=>["tmv", "antigen", "immunohistochemistry", "lungs", "tmv-inoculated"], "article_id"=>169454, "categories"=>["Virology", "Microbiology", "Plant Biology"], "users"=>["Fanny Balique", "Philippe Colson", "Abdoulaye Oury Barry", "Claude Nappez", "Audrey Ferretti", "Khatoun Al Moussawi", "Tatsiana Ngounga", "Hubert Lepidi", "Eric Ghigo", "Jean-Louis Mege", "Hervé Lecoq", "Didier Raoult"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0054993.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Detection_of_TMV_antigen_by_immunohistochemistry_in_lungs_of_TMV_inoculated_mice_/169454", "title"=>"Detection of TMV antigen by immunohistochemistry in lungs of TMV-inoculated mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 16:17:15"}
  • {"files"=>["https://ndownloader.figshare.com/files/499084"], "description"=>"<p>Detection of anti-TMV total antibodies in serum samples of 10 TMV-inoculated and 5 control mice at day 0 (just before the operation), and 13 TMV-inoculated and 8 control mice at day 7 and day 14 after intratracheal inoculation. *Statistically significant.</p>", "links"=>[], "tags"=>["antibody", "serum"], "article_id"=>169594, "categories"=>["Virology", "Microbiology", "Plant Biology"], "users"=>["Fanny Balique", "Philippe Colson", "Abdoulaye Oury Barry", "Claude Nappez", "Audrey Ferretti", "Khatoun Al Moussawi", "Tatsiana Ngounga", "Hubert Lepidi", "Eric Ghigo", "Jean-Louis Mege", "Hervé Lecoq", "Didier Raoult"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0054993.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Anti_TMV_antibody_testing_in_mouse_serum_samples_/169594", "title"=>"Anti-TMV antibody testing in mouse serum samples.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 16:18:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/499235"], "description"=>"<p>A: TMV coat protein gene system; B: TMV replicase gene system. Control, non-inoculated mice.</p>", "links"=>[], "tags"=>["rna", "quantification", "lungs", "Real-time"], "article_id"=>169741, "categories"=>["Virology", "Microbiology", "Plant Biology"], "users"=>["Fanny Balique", "Philippe Colson", "Abdoulaye Oury Barry", "Claude Nappez", "Audrey Ferretti", "Khatoun Al Moussawi", "Tatsiana Ngounga", "Hubert Lepidi", "Eric Ghigo", "Jean-Louis Mege", "Hervé Lecoq", "Didier Raoult"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0054993.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_TMV_RNA_quantification_in_mouse_lungs_by_real_time_RT_PCR_/169741", "title"=>"TMV RNA quantification in mouse lungs by real-time RT-PCR.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 16:18:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/499379"], "description"=>"<p>Arrows indicate TMV-like particles.</p>", "links"=>[], "tags"=>["particles", "observed", "coloration", "electron", "microscopy", "homogenate", "inoculated", "days"], "article_id"=>169876, "categories"=>["Virology", "Microbiology", "Plant Biology"], "users"=>["Fanny Balique", "Philippe Colson", "Abdoulaye Oury Barry", "Claude Nappez", "Audrey Ferretti", "Khatoun Al Moussawi", "Tatsiana Ngounga", "Hubert Lepidi", "Eric Ghigo", "Jean-Louis Mege", "Hervé Lecoq", "Didier Raoult"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0054993.g005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_TMV_like_particles_observed_by_negative_coloration_on_electron_microscopy_in_the_homogenate_of_one_inoculated_lung_sample_1_day_A_and_3_days_B_post_inoculation_/169876", "title"=>"TMV-like particles observed by negative coloration on electron microscopy in the homogenate of one inoculated lung sample 1 day (A) and 3 days (B) post-inoculation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 16:19:42"}
  • {"files"=>["https://ndownloader.figshare.com/files/499513"], "description"=>"<p>A: Leaves of a non-inoculated plant; B: Leaves of a plant inoculated with a TMV RNA positive sample (TMV-inoculated mouse number 1 at day 3). Arrows show signs of systemic infection with discoloration and deformation of young leaves.</p>", "links"=>[], "tags"=>["tmv", "rna-positive", "samples"], "article_id"=>170018, "categories"=>["Virology", "Microbiology", "Plant Biology"], "users"=>["Fanny Balique", "Philippe Colson", "Abdoulaye Oury Barry", "Claude Nappez", "Audrey Ferretti", "Khatoun Al Moussawi", "Tatsiana Ngounga", "Hubert Lepidi", "Eric Ghigo", "Jean-Louis Mege", "Hervé Lecoq", "Didier Raoult"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0054993.g006"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Infectivity_test_for_TMV_RNA_positive_lung_samples_on_Nicotiana_benthamiana_/170018", "title"=>"Infectivity test for TMV RNA-positive lung samples on <i>Nicotiana benthamiana</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 16:20:30"}
  • {"files"=>["https://ndownloader.figshare.com/files/499677"], "description"=>"<p>A: Leaf of a non-inoculated plant; B: Leaf of a plant inoculated with a TMV RNA positive sample (TMV-inoculated mouse number 2 at day1); C: Leaf of a plant inoculated with a TMV RNA positive sample (TMV-inoculated mouse number 1 at day 3). On inoculated leaves, arrows show local necrotic lesions induced by the virus in the plant cells.</p>", "links"=>[], "tags"=>["tmv", "rna-positive", "samples"], "article_id"=>170180, "categories"=>["Virology", "Microbiology", "Plant Biology"], "users"=>["Fanny Balique", "Philippe Colson", "Abdoulaye Oury Barry", "Claude Nappez", "Audrey Ferretti", "Khatoun Al Moussawi", "Tatsiana Ngounga", "Hubert Lepidi", "Eric Ghigo", "Jean-Louis Mege", "Hervé Lecoq", "Didier Raoult"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0054993.g007"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Infectivity_test_for_TMV_RNA_positive_lung_samples_on_Nicotiana_tabacum_Xanthi_/170180", "title"=>"Infectivity test for TMV RNA-positive lung samples on <i>Nicotiana tabacum</i> Xanthi.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 16:21:24"}
  • {"files"=>["https://ndownloader.figshare.com/files/499814"], "description"=>"<p>The PCR systems targets the coat protein gene.</p>", "links"=>[], "tags"=>["rna", "quantification", "Real-time", "pcr", "bronchoalveolar", "lavage", "macrophages", "tmv-inoculated"], "article_id"=>170317, "categories"=>["Virology", "Microbiology", "Plant Biology"], "users"=>["Fanny Balique", "Philippe Colson", "Abdoulaye Oury Barry", "Claude Nappez", "Audrey Ferretti", "Khatoun Al Moussawi", "Tatsiana Ngounga", "Hubert Lepidi", "Eric Ghigo", "Jean-Louis Mege", "Hervé Lecoq", "Didier Raoult"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0054993.g008"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_TMV_RNA_quantification_by_real_time_PCR_in_bronchoalveolar_lavage_macrophages_of_TMV_inoculated_and_control_mice_/170317", "title"=>"TMV RNA quantification by real-time PCR in bronchoalveolar lavage macrophages of TMV-inoculated and control mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 16:22:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/499969"], "description"=>"<p>BMDM were observed at day 1 (A and B), day 7 (C and D) and 14 (E and F) after inoculation with TMV. Non-inoculated cells (A, C, E) and TMV-inoculated cells (B, D, E) were analyzed using a mouse monoclonal anti-TMV antibody diluted at 1∶25 with goat anti-mouse 555 antibodies (red). Actin is stained with phallacidin-488 (green) and nuclei are stained with DAPI (blue). Arrows show TMV antigen (red) detected in BMDM cytoplasm. BMDM were observed by confocal microscopy with an oil immersion objective of 63X and a digital zoom of 2.</p>", "links"=>[], "tags"=>["marrow", "derived", "macrophages"], "article_id"=>170474, "categories"=>["Virology", "Microbiology", "Plant Biology"], "users"=>["Fanny Balique", "Philippe Colson", "Abdoulaye Oury Barry", "Claude Nappez", "Audrey Ferretti", "Khatoun Al Moussawi", "Tatsiana Ngounga", "Hubert Lepidi", "Eric Ghigo", "Jean-Louis Mege", "Hervé Lecoq", "Didier Raoult"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0054993.g009"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Immunofluorescence_analysis_of_bone_marrow_derived_macrophages_BMDM_/170474", "title"=>"Immunofluorescence analysis of bone marrow derived macrophages (BMDM).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 16:23:00"}
  • {"files"=>["https://ndownloader.figshare.com/files/500141"], "description"=>"<p>Quantification of the TMV coat protein encoding RNA region in BMDM culture medium (A) and within cells (B) inoculated with 0, 10<sup>7</sup>,10<sup>9</sup> or10<sup>11</sup>TMV particles during 15 days.</p>", "links"=>[], "tags"=>["rna", "quantification", "Real-time", "pcr", "marrow-derived", "macrophages"], "article_id"=>170646, "categories"=>["Virology", "Microbiology", "Plant Biology"], "users"=>["Fanny Balique", "Philippe Colson", "Abdoulaye Oury Barry", "Claude Nappez", "Audrey Ferretti", "Khatoun Al Moussawi", "Tatsiana Ngounga", "Hubert Lepidi", "Eric Ghigo", "Jean-Louis Mege", "Hervé Lecoq", "Didier Raoult"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0054993.g010"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_TMV_RNA_quantification_by_real_time_PCR_in_bone_marrow_derived_macrophages_BMDM_/170646", "title"=>"TMV RNA quantification by real-time PCR in bone marrow-derived macrophages (BMDM).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 16:23:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/500265"], "description"=>"a<p>in reference to sequence GenBank accession no. AB369276; nt, nucleotide.</p>", "links"=>[], "tags"=>["primers", "rt-pcr"], "article_id"=>170771, "categories"=>["Virology", "Microbiology", "Plant Biology"], "users"=>["Fanny Balique", "Philippe Colson", "Abdoulaye Oury Barry", "Claude Nappez", "Audrey Ferretti", "Khatoun Al Moussawi", "Tatsiana Ngounga", "Hubert Lepidi", "Eric Ghigo", "Jean-Louis Mege", "Hervé Lecoq", "Didier Raoult"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0054993.t001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Probes_and_primers_used_for_RT_PCR_assays_/170771", "title"=>"Probes and primers used for RT-PCR assays.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-19 16:24:38"}

PMC Usage Stats | Further Information

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Relative Metric

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