Nanostructured 3D Constructs Based on Chitosan and Chondroitin Sulphate Multilayers for Cartilage Tissue Engineering
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{"title"=>"Nanostructured 3D Constructs Based on Chitosan and Chondroitin Sulphate Multilayers for Cartilage Tissue Engineering", "type"=>"journal", "authors"=>[{"first_name"=>"Joana M.", "last_name"=>"Silva", "scopus_author_id"=>"56719861800"}, {"first_name"=>"Nicole", "last_name"=>"Georgi", "scopus_author_id"=>"35421936000"}, {"first_name"=>"Rui", "last_name"=>"Costa", "scopus_author_id"=>"7203063544"}, {"first_name"=>"Praveen", "last_name"=>"Sher", "scopus_author_id"=>"10141383200"}, {"first_name"=>"Rui L.", "last_name"=>"Reis", "scopus_author_id"=>"56861715700"}, {"first_name"=>"Clemens A.", "last_name"=>"van Blitterswijk", "scopus_author_id"=>"7006225712"}, {"first_name"=>"Marcel", "last_name"=>"Karperien", "scopus_author_id"=>"7004301928"}, {"first_name"=>"João F.", "last_name"=>"Mano", "scopus_author_id"=>"26643558900"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84874247954", "sgr"=>"84874247954", "pui"=>"368400200", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pmid"=>"23437056", "doi"=>"10.1371/journal.pone.0055451"}, "id"=>"9e2ff201-5380-3d6b-9136-99e6641ad380", "abstract"=>"Nanostructured three-dimensional constructs combining layer-by-layer technology (LbL) and template leaching were processed and evaluated as possible support structures for cartilage tissue engineering. Multilayered constructs were formed by depositing the polyelectrolytes chitosan (CHT) and chondroitin sulphate (CS) on either bidimensional glass surfaces or 3D packet of paraffin spheres. 2D CHT/CS multi-layered constructs proved to support the attachment and proliferation of bovine chondrocytes (BCH). The technology was transposed to 3D level and CHT/CS multi-layered hierarchical scaffolds were retrieved after paraffin leaching. The obtained nanostructured 3D constructs had a high porosity and water uptake capacity of about 300%. Dynamical mechanical analysis (DMA) showed the viscoelastic nature of the scaffolds. Cellular tests were performed with the culture of BCH and multipotent bone marrow derived stromal cells (hMSCs) up to 21 days in chondrogenic differentiation media. Together with scanning electronic microscopy analysis, viability tests and DNA quantification, our results clearly showed that cells attached, proliferated and were metabolically active over the entire scaffold. Cartilaginous extracellular matrix (ECM) formation was further assessed and results showed that GAG secretion occurred indicating the maintenance of the chondrogenic phenotype and the chondrogenic differentiation of hMSCs.", "link"=>"http://www.mendeley.com/research/nanostructured-3d-constructs-based-chitosan-chondroitin-sulphate-multilayers-cartilage-tissue-engine", "reader_count"=>87, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>3, "Researcher"=>16, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>25, "Student > Postgraduate"=>4, "Other"=>2, "Student > Master"=>18, "Student > Bachelor"=>8, "Lecturer"=>2, "Professor"=>5}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>3, "Researcher"=>16, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>25, "Student > Postgraduate"=>4, "Other"=>2, "Student > Master"=>18, "Student > Bachelor"=>8, "Lecturer"=>2, "Professor"=>5}, "reader_count_by_subject_area"=>{"Unspecified"=>4, "Agricultural and Biological Sciences"=>27, "Business, Management and Accounting"=>1, "Chemistry"=>9, "Engineering"=>18, "Environmental Science"=>2, "Biochemistry, Genetics and Molecular Biology"=>8, "Materials Science"=>10, "Medicine and Dentistry"=>4, "Sports and Recreations"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Physics and Astronomy"=>1, "Social Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Materials Science"=>{"Materials Science"=>10}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Social Sciences"=>{"Social Sciences"=>1}, "Sports and Recreations"=>{"Sports and Recreations"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Unspecified"=>{"Unspecified"=>4}, "Environmental Science"=>{"Environmental Science"=>2}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "Engineering"=>{"Engineering"=>18}, "Chemistry"=>{"Chemistry"=>9}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>27}, "Business, Management and Accounting"=>{"Business, Management and Accounting"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>8}}, "reader_count_by_country"=>{"Netherlands"=>1, "United States"=>1, "Ireland"=>2, "Portugal"=>2, "Germany"=>2}, "group_count"=>6}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/957003"], "description"=>"<p>Significant differences between each cell type at different time points were found for p<0.05(*) and p<0.01(**).</p>", "links"=>[], "tags"=>["assay", "scaffolds", "seeded", "bch", "hmscs", "differentiation"], "article_id"=>627245, "categories"=>["Biotechnology", "Biochemistry", "Ecology", "Cell Biology"], "users"=>["Joana M. Silva", "Nicole Georgi", "Rui Costa", "Praveen Sher", "Rui L. Reis", "Clemens A. van Blitterswijk", "Marcel Karperien", "João F. Mano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0055451.g008", "stats"=>{"downloads"=>1, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_DNA_assay_on_the_scaffolds_seeded_with_BCH_and_hMSCs_in_differentiation_medium_/627245", "title"=>"DNA assay on the scaffolds seeded with BCH and hMSCs in differentiation medium.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 02:00:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/956999"], "description"=>"<p>Live/dead assay, MTT assay and cross-section SEM micrographs of hMSCs seeded on scaffold at day 1(A, B, C), 3(D, E, F), 14 (G, H, I) and 21(J, K, L) of culture in proliferation medium.</p>", "links"=>[], "tags"=>["mtt", "assay", "cross-section", "sem", "micrographs", "hmscs", "seeded", "scaffold", "14", "proliferation"], "article_id"=>627244, "categories"=>["Biotechnology", "Biochemistry", "Ecology", "Cell Biology"], "users"=>["Joana M. Silva", "Nicole Georgi", "Rui Costa", "Praveen Sher", "Rui L. Reis", "Clemens A. van Blitterswijk", "Marcel Karperien", "João F. Mano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0055451.g007", "stats"=>{"downloads"=>1, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Live_dead_assay_MTT_assay_and_cross_section_SEM_micrographs_of_hMSCs_seeded_on_scaffold_at_day_1_A_B_C_3_D_E_F_14_G_H_I_and_21_J_K_L_of_culture_in_proliferation_medium_/627244", "title"=>"Live/dead assay, MTT assay and cross-section SEM micrographs of hMSCs seeded on scaffold at day 1(A, B, C), 3(D, E, F), 14 (G, H, I) and 21(J, K, L) of culture in proliferation medium.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 02:00:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/956991"], "description"=>"<p>Experiments are reported for dry samples (▪) and hydrated samples in PBS at 37°C (•).</p>", "links"=>[], "tags"=>["modulus", "scaffolds", "lbl"], "article_id"=>627241, "categories"=>["Biotechnology", "Biochemistry", "Ecology", "Cell Biology"], "users"=>["Joana M. Silva", "Nicole Georgi", "Rui Costa", "Praveen Sher", "Rui L. Reis", "Clemens A. van Blitterswijk", "Marcel Karperien", "João F. Mano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0055451.g005", "stats"=>{"downloads"=>1, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Variations_of_A_Storage_modulus_E_and_b_loss_factor_tan_948_of_the_CHT_CS_scaffolds_obtained_by_LbL_methodology_/627241", "title"=>"Variations of (A) Storage modulus (E') and (b) loss factor (tanδ) of the CHT/CS scaffolds obtained by LbL methodology.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 02:00:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/956989"], "description"=>"<p>A) FTIR measurements of CHT/CS scaffolds and pure polysaccharides (CHT and CS), B) Swelling test up to 3 days (The inset graphic expands the water uptake for the first 5 hours), C) Weight loss of the scaffolds in PBS (▴) and in an enzymatic solution at 37°C (▪).</p>", "links"=>[], "tags"=>["characterization"], "article_id"=>627239, "categories"=>["Biotechnology", "Biochemistry", "Ecology", "Cell Biology"], "users"=>["Joana M. Silva", "Nicole Georgi", "Rui Costa", "Praveen Sher", "Rui L. Reis", "Clemens A. van Blitterswijk", "Marcel Karperien", "João F. Mano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0055451.g004", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Physicochemical_characterization_of_scaffolds_/627239", "title"=>"Physicochemical characterization of scaffolds.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 02:00:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/956983"], "description"=>"<p>Live/dead assay and SEM micrographs of BCH seeded on glass coverslips coated with chitosan and chondroitin sulphate at day 1 (A, B, C), 3 (D, E, F), 7 (G, H, I), 14 (J, K, L) and 21 (M, N) of culture in proliferation medium.</p>", "links"=>[], "tags"=>["assay", "sem", "micrographs", "bch", "seeded", "coverslips", "coated", "chitosan", "chondroitin", "sulphate", "14", "21", "proliferation"], "article_id"=>627234, "categories"=>["Biotechnology", "Biochemistry", "Ecology", "Cell Biology"], "users"=>["Joana M. Silva", "Nicole Georgi", "Rui Costa", "Praveen Sher", "Rui L. Reis", "Clemens A. van Blitterswijk", "Marcel Karperien", "João F. Mano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0055451.g002", "stats"=>{"downloads"=>1, "page_views"=>29, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Live_dead_assay_and_SEM_micrographs_of_BCH_seeded_on_glass_coverslips_coated_with_chitosan_and_chondroitin_sulphate_at_day_1_A_B_C_3_D_E_F_7_G_H_I_14_J_K_L_and_21_M_N_of_culture_in_proliferation_medium_/627234", "title"=>"Live/dead assay and SEM micrographs of BCH seeded on glass coverslips coated with chitosan and chondroitin sulphate at day 1 (A, B, C), 3 (D, E, F), 7 (G, H, I), 14 (J, K, L) and 21 (M, N) of culture in proliferation medium.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 02:00:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/957009"], "description"=>"<div><p>Nanostructured three-dimensional constructs combining layer-by-layer technology (LbL) and template leaching were processed and evaluated as possible support structures for cartilage tissue engineering. Multilayered constructs were formed by depositing the polyelectrolytes chitosan (CHT) and chondroitin sulphate (CS) on either bidimensional glass surfaces or 3D packet of paraffin spheres. 2D CHT/CS multi-layered constructs proved to support the attachment and proliferation of bovine chondrocytes (BCH). The technology was transposed to 3D level and CHT/CS multi-layered hierarchical scaffolds were retrieved after paraffin leaching. The obtained nanostructured 3D constructs had a high porosity and water uptake capacity of about 300%. Dynamical mechanical analysis (DMA) showed the viscoelastic nature of the scaffolds. Cellular tests were performed with the culture of BCH and multipotent bone marrow derived stromal cells (hMSCs) up to 21 days in chondrogenic differentiation media. Together with scanning electronic microscopy analysis, viability tests and DNA quantification, our results clearly showed that cells attached, proliferated and were metabolically active over the entire scaffold. Cartilaginous extracellular matrix (ECM) formation was further assessed and results showed that GAG secretion occurred indicating the maintenance of the chondrogenic phenotype and the chondrogenic differentiation of hMSCs.</p> </div>", "links"=>[], "tags"=>["nanostructured", "3d", "constructs", "chitosan", "chondroitin", "sulphate", "multilayers", "cartilage", "engineering"], "article_id"=>627248, "categories"=>["Biotechnology", "Biochemistry", "Ecology", "Cell Biology"], "users"=>["Joana M. Silva", "Nicole Georgi", "Rui Costa", "Praveen Sher", "Rui L. Reis", "Clemens A. van Blitterswijk", "Marcel Karperien", "João F. Mano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0055451", "stats"=>{"downloads"=>1, "page_views"=>43, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Nanostructured_3D_Constructs_Based_on_Chitosan_and_Chondroitin_Sulphate_Multilayers_for_Cartilage_Tissue_Engineering__/627248", "title"=>"Nanostructured 3D Constructs Based on Chitosan and Chondroitin Sulphate Multilayers for Cartilage Tissue Engineering", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 02:00:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/957005"], "description"=>"<p>Histological cross-sections of scaffolds seeded with BCH and hMSCs stained by H&E and Alcian blue at different days of culture in differentiation medium.</p>", "links"=>[], "tags"=>["cross-sections", "scaffolds", "seeded", "bch", "hmscs", "stained", "alcian", "days", "differentiation"], "article_id"=>627246, "categories"=>["Biotechnology", "Biochemistry", "Ecology", "Cell Biology"], "users"=>["Joana M. Silva", "Nicole Georgi", "Rui Costa", "Praveen Sher", "Rui L. Reis", "Clemens A. van Blitterswijk", "Marcel Karperien", "João F. Mano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0055451.g009", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Histological_cross_sections_of_scaffolds_seeded_with_BCH_and_hMSCs_stained_by_H_amp_E_and_Alcian_blue_at_different_days_of_culture_in_differentiation_medium_/627246", "title"=>"Histological cross-sections of scaffolds seeded with BCH and hMSCs stained by H&E and Alcian blue at different days of culture in differentiation medium.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 02:00:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/956993"], "description"=>"<p>Live/dead assay, MTT assay and cross-section SEM micrographs of BCH seeded on scaffold at day 1(A, B, C), 3(D, E, F), 14 (G, H, I) and 21(J, K, L) of culture in proliferation medium.</p>", "links"=>[], "tags"=>["mtt", "assay", "cross-section", "sem", "micrographs", "bch", "seeded", "scaffold", "14", "proliferation"], "article_id"=>627242, "categories"=>["Biotechnology", "Biochemistry", "Ecology", "Cell Biology"], "users"=>["Joana M. Silva", "Nicole Georgi", "Rui Costa", "Praveen Sher", "Rui L. Reis", "Clemens A. van Blitterswijk", "Marcel Karperien", "João F. Mano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0055451.g006", "stats"=>{"downloads"=>4, "page_views"=>75, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Live_dead_assay_MTT_assay_and_cross_section_SEM_micrographs_of_BCH_seeded_on_scaffold_at_day_1_A_B_C_3_D_E_F_14_G_H_I_and_21_J_K_L_of_culture_in_proliferation_medium_/627242", "title"=>"Live/dead assay, MTT assay and cross-section SEM micrographs of BCH seeded on scaffold at day 1(A, B, C), 3(D, E, F), 14 (G, H, I) and 21(J, K, L) of culture in proliferation medium.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 02:00:42"}
  • {"files"=>["https://ndownloader.figshare.com/files/956986"], "description"=>"<p>A) Production steps of scaffolds: LbL and leaching of free-packet paraffin spheres, B)Digital photograph of the scaffold after all the steps C) Optical Microscopy image of the scaffolds after the leaching of the core material, D, E) SEM micrographs of cross-sections (two different magnifications) and Histological cross-sections of the scaffolds after staining with alcian blue (F) and eosin (G).</p>", "links"=>[], "tags"=>["biotechnology", "cell biology", "rheumatology", "Biochemistry"], "article_id"=>627237, "categories"=>["Biotechnology", "Biochemistry", "Ecology", "Cell Biology"], "users"=>["Joana M. Silva", "Nicole Georgi", "Rui Costa", "Praveen Sher", "Rui L. Reis", "Clemens A. van Blitterswijk", "Marcel Karperien", "João F. Mano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0055451.g003", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Scaffold_characterization_/627237", "title"=>"Scaffold characterization.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 02:00:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/956979"], "description"=>"<p>A) Normalized frequency (?f<sub>7</sub>/7) and dissipation changes (?D<sub>7</sub>) obtain at 35 MHz, 1) deposition of CHT, 2) washing step and 3) deposition of CS; B) Estimated thickness (th) evolution and SEM micrographs of the multilayer surface with 10 double layers (inset image).</p>", "links"=>[], "tags"=>["monitoring", "polyelectrolyte", "multi-layered", "qcm"], "article_id"=>627231, "categories"=>["Biotechnology", "Biochemistry", "Ecology", "Cell Biology"], "users"=>["Joana M. Silva", "Nicole Georgi", "Rui Costa", "Praveen Sher", "Rui L. Reis", "Clemens A. van Blitterswijk", "Marcel Karperien", "João F. Mano"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0055451.g001", "stats"=>{"downloads"=>2, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Build_up_monitoring_of_the_CHT_CS_polyelectrolyte_multi_layered_using_QCM_for_film_constructed_/627231", "title"=>"Build-up monitoring of the CHT/CS polyelectrolyte multi-layered using QCM for film constructed.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 02:00:31"}

PMC Usage Stats | Further Information

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Relative Metric

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