Protein Arginine Methylation Is More Prone to Inhibition by S-Adenosylhomocysteine than DNA Methylation in Vascular Endothelial Cells
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{"title"=>"Protein Arginine Methylation Is More Prone to Inhibition by S-Adenosylhomocysteine than DNA Methylation in Vascular Endothelial Cells", "type"=>"journal", "authors"=>[{"first_name"=>"Ruben", "last_name"=>"Esse", "scopus_author_id"=>"36027354900"}, {"first_name"=>"Monica S.", "last_name"=>"Rocha", "scopus_author_id"=>"57198190078"}, {"first_name"=>"Madalena", "last_name"=>"Barroso", "scopus_author_id"=>"57200741445"}, {"first_name"=>"Cristina", "last_name"=>"Florindo", "scopus_author_id"=>"56189060300"}, {"first_name"=>"Tom", "last_name"=>"Teerlink", "scopus_author_id"=>"7006882998"}, {"first_name"=>"Robert M.", "last_name"=>"Kok", "scopus_author_id"=>"24556546600"}, {"first_name"=>"Yvo M.", "last_name"=>"Smulders", "scopus_author_id"=>"7004624488"}, {"first_name"=>"Isabel", "last_name"=>"Rivera", "scopus_author_id"=>"7004389683"}, {"first_name"=>"Paula", "last_name"=>"Leandro", "scopus_author_id"=>"6602522134"}, {"first_name"=>"Pieter", "last_name"=>"Koolwijk", "scopus_author_id"=>"7004086500"}, {"first_name"=>"Rita", "last_name"=>"Castro", "scopus_author_id"=>"55934294200"}, {"first_name"=>"Henk J.", "last_name"=>"Blom", "scopus_author_id"=>"7101946367"}, {"first_name"=>"Isabel Tavares", "last_name"=>"de Almeida", "scopus_author_id"=>"7004144157"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84873651491", "sgr"=>"84873651491", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0055483", "pmid"=>"23408989", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pui"=>"368325926"}, "id"=>"c4b1bc04-857d-38ac-9108-10996454d485", "abstract"=>"Methyltransferases use S-adenosylmethionine (AdoMet) as methyl group donor, forming S-adenosylhomocysteine (AdoHcy) and methylated substrates, including DNA and proteins. AdoHcy inhibits most methyltransferases. Accumulation of intracellular AdoHcy secondary to Hcy elevation elicits global DNA hypomethylation. We aimed at determining the extent at which protein arginine methylation status is affected by accumulation of intracellular AdoHcy. AdoHcy accumulation in human umbilical vein endothelial cells was induced by inhibition of AdoHcy hydrolase by adenosine-2,3-dialdehyde (AdOx). As a measure of protein arginine methylation status, the levels of monomethylarginine (MMA) and asymmetric and symmetric dimethylated arginine residues (ADMA and SDMA, respectively) in cell protein hydrolysates were measured by HPLC. A 10% decrease was observed at a 2.5-fold increase of intracellular AdoHcy. Western blotting revealed that the translational levels of the main enzymes catalyzing protein arginine methylation, protein arginine methyl transferases (PRMTs) 1 and 5, were not affected by AdoHcy accumulation. Global DNA methylation status was evaluated by measuring 5-methylcytosine and total cytosine concentrations in DNA hydrolysates by LC-MS/MS. DNA methylation decreased by 10% only when intracellular AdoHcy concentration accumulated to 6-fold of its basal value. In conclusion, our results indicate that protein arginine methylation is more sensitive to AdoHcy accumulation than DNA methylation, pinpointing a possible new player in methylation-related pathology.", "link"=>"http://www.mendeley.com/research/protein-arginine-methylation-more-prone-inhibition-sadenosylhomocysteine-dna-methylation-vascular-en", "reader_count"=>38, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>4, "Librarian"=>2, "Student > Doctoral Student"=>1, "Researcher"=>6, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>2, "Student > Master"=>9, "Student > Bachelor"=>2, "Lecturer"=>2, "Lecturer > Senior Lecturer"=>1, "Professor"=>2}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>4, "Librarian"=>2, "Student > Doctoral Student"=>1, "Researcher"=>6, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>2, "Student > Master"=>9, "Student > Bachelor"=>2, "Lecturer"=>2, "Lecturer > Senior Lecturer"=>1, "Professor"=>2}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>12, "Agricultural and Biological Sciences"=>13, "Medicine and Dentistry"=>6, "Pharmacology, Toxicology and Pharmaceutical Science"=>3, "Chemical Engineering"=>1, "Chemistry"=>2, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>6}, "Chemistry"=>{"Chemistry"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>13}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>12}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>3}, "Chemical Engineering"=>{"Chemical Engineering"=>1}}, "reader_count_by_country"=>{"Argentina"=>1, "United States"=>1, "Germany"=>1}, "group_count"=>3}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/491665"], "description"=>"<p>(<b>A</b>) Real-time RT-PCR analysis was performed in triplicate for quantification of mRNA levels of <i>PRMT1</i>, <i>PRMT5</i>, <i>PRMT4</i> and <i>PRMT7</i> relative to control, using <i>EIF4A2</i> as reference gene. Data are mean±SD. (<b>B</b>) Protein levels of PRMT1 and PRMT5 determined by Western blotting, using β-actin as internal control. A representative blot is shown. Densitometry was performed on 3 blots obtained from independent experiments. Data are mean±SD. Statistical significance (<i>P</i>) was determined using the Student’s paired t-test; <i>P</i> = NS for trend and *<i>P</i><0.05, <i>versus</i> control.</p>", "links"=>[], "tags"=>["prmt1", "prmt5", "24"], "article_id"=>162168, "categories"=>["Molecular Biology", "Biochemistry", "Cell Biology", "Genetics", "Biophysics"], "users"=>["Ruben Esse", "Monica S. Rocha", "Madalena Barroso", "Cristina Florindo", "Tom Teerlink", "Robert M. Kok", "Yvo M. Smulders", "Isabel Rivera", "Paula Leandro", "Pieter Koolwijk", "Rita Castro", "Henk J. Blom", "Isabel Tavares de Almeida"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0055483.g004", "stats"=>{"downloads"=>3, "page_views"=>22, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Relative_PRMT1_and_PRMT5_expression_in_HUVEC_after_24_hours_of_incubation_in_the_absence_and_presence_of_AdOx_/162168", "title"=>"Relative PRMT1 and PRMT5 expression in HUVEC, after 24 hours of incubation, in the absence and presence of AdOx.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 15:38:03"}
  • {"files"=>["https://ndownloader.figshare.com/files/491581"], "description"=>"<p>Data are medians, quartiles, and extreme values and represent 5 to 7 independent experiments performed with HUVEC obtained from individual donors. Statistical significance (<i>P</i>) was determined using the Student’s paired t-test; *<i>P</i><0.05, **<i>P</i><0.01 and ***<i>P</i><0.001, <i>versus</i> control.</p>", "links"=>[], "tags"=>["adox", "aza", "arginine", "methylation", "evaluated", "ratios", "dimethylated", "24"], "article_id"=>162077, "categories"=>["Molecular Biology", "Biochemistry", "Cell Biology", "Genetics", "Biophysics"], "users"=>["Ruben Esse", "Monica S. Rocha", "Madalena Barroso", "Cristina Florindo", "Tom Teerlink", "Robert M. Kok", "Yvo M. Smulders", "Isabel Rivera", "Paula Leandro", "Pieter Koolwijk", "Rita Castro", "Henk J. Blom", "Isabel Tavares de Almeida"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0055483.g003", "stats"=>{"downloads"=>1, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_AdOx_and_AZA_treatment_on_protein_arginine_methylation_status_in_HUVEC_evaluated_as_the_ratios_of_dimethylated_arginine_residues_total_arginine_in_protein_hydrolysates_after_24_hours_of_incubation_in_the_absence_and_presence_of_AdOx_or_AZA_/162077", "title"=>"Effect of AdOx and AZA treatment on protein arginine methylation status in HUVEC, evaluated as the ratios of dimethylated arginine residues/total arginine in protein hydrolysates, after 24 hours of incubation, in the absence and presence of AdOx or AZA.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 15:37:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/491752"], "description"=>"<p>Data represent 7 independent experiments with HUVEC from individual donors. Basal levels of ADMA and SDMA in fresh medium were subtracted. Statistical significance (<i>P</i>) was determined using the Student’s paired t-test; **<i>P</i><0.01, <i>versus</i> control. IQR = interquartile range.</p>", "links"=>[], "tags"=>["adma", "sdma", "huvec", "24"], "article_id"=>162255, "categories"=>["Molecular Biology", "Biochemistry", "Cell Biology", "Genetics", "Biophysics"], "users"=>["Ruben Esse", "Monica S. Rocha", "Madalena Barroso", "Cristina Florindo", "Tom Teerlink", "Robert M. Kok", "Yvo M. Smulders", "Isabel Rivera", "Paula Leandro", "Pieter Koolwijk", "Rita Castro", "Henk J. Blom", "Isabel Tavares de Almeida"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0055483.t001", "stats"=>{"downloads"=>4, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Concentrations_of_ADMA_and_SDMA_in_HUVEC_culture_medium_after_24_hours_of_incubation_in_the_absence_and_presence_of_AdOx_/162255", "title"=>"Concentrations of ADMA and SDMA in HUVEC culture medium, after 24 hours of incubation, in the absence and presence of AdOx.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-19 15:38:30"}
  • {"files"=>["https://ndownloader.figshare.com/files/491495"], "description"=>"<p>Data are medians, quartiles, and extreme values and represent 6 to 16 independent experiments performed with HUVEC obtained from different donors. Statistical significance (<i>P</i>) was determined using the Student’s paired t-test; **<i>P</i><0.01 and ***<i>P</i><0.001, <i>versus</i> control.</p>", "links"=>[], "tags"=>["adox", "aza", "genomic", "dna", "methylation", "evaluated", "cytosine"], "article_id"=>162001, "categories"=>["Molecular Biology", "Biochemistry", "Cell Biology", "Genetics", "Biophysics"], "users"=>["Ruben Esse", "Monica S. Rocha", "Madalena Barroso", "Cristina Florindo", "Tom Teerlink", "Robert M. Kok", "Yvo M. Smulders", "Isabel Rivera", "Paula Leandro", "Pieter Koolwijk", "Rita Castro", "Henk J. Blom", "Isabel Tavares de Almeida"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0055483.g002", "stats"=>{"downloads"=>3, "page_views"=>33, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_AdOx_and_AZA_treatment_on_genomic_DNA_methylation_status_in_HUVEC_evaluated_as_the_ratio_5_methylcytosine_total_cytosine_mC_tC_/162001", "title"=>"Effect of AdOx and AZA treatment on genomic DNA methylation status in HUVEC, evaluated as the ratio 5-methylcytosine/total cytosine (mC/tC).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 15:37:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/491377"], "description"=>"<p>Data represent the intracellular concentration of AdoMet (A) and AdoHcy (B) and the concentration of tHcy in the incubation medium (C) after 24 hours of incubation in the absence and presence of AdOx or AZA (see text for details), expressed as percentage of control. Data are medians, quartiles, and extreme values and represent 6 to 13 independent experiments performed with HUVEC obtained from individual donors. Statistical significance (<i>P</i>) was determined using the Student’s paired t-test; **<i>P</i><0.01 and ***<i>P</i><0.001, <i>versus</i> control.</p>", "links"=>[], "tags"=>["huvec", "inhibitor", "adohcy", "induces", "intracellular", "accumulation", "lowers", "thcy"], "article_id"=>161871, "categories"=>["Molecular Biology", "Biochemistry", "Cell Biology", "Genetics", "Biophysics"], "users"=>["Ruben Esse", "Monica S. Rocha", "Madalena Barroso", "Cristina Florindo", "Tom Teerlink", "Robert M. Kok", "Yvo M. Smulders", "Isabel Rivera", "Paula Leandro", "Pieter Koolwijk", "Rita Castro", "Henk J. Blom", "Isabel Tavares de Almeida"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0055483.g001", "stats"=>{"downloads"=>2, "page_views"=>25, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Incubation_of_HUVEC_with_AdOx_an_inhibitor_of_AdoHcy_hydrolase_induces_intracellular_AdoHcy_accumulation_and_lowers_tHcy_production_/161871", "title"=>"Incubation of HUVEC with AdOx, an inhibitor of AdoHcy hydrolase, induces intracellular AdoHcy accumulation and lowers tHcy production.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 15:36:21"}

PMC Usage Stats | Further Information

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  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
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  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
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Relative Metric

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