Simultaneous Correlative Scanning Electron and High-NA Fluorescence Microscopy
Publication Date
February 08, 2013
Journal
PLOS ONE
Authors
Nalan Liv, A. Christiaan Zonnevylle, Angela C. Narvaez, Andries P. J. Effting, et al
Volume
8
Issue
2
Pages
e55707
DOI
https://dx.plos.org/10.1371/journal.pone.0055707
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0055707
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/23409024
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3568124
Europe PMC
http://europepmc.org/abstract/MED/23409024
Web of Science
000314660300030
Scopus
84873694839
Mendeley
http://www.mendeley.com/research/simultaneous-correlative-scanning-electron-highna-fluorescence-microscopy
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Simultaneous Correlative Scanning Electron and High-NA Fluorescence Microscopy", "type"=>"journal", "authors"=>[{"first_name"=>"Nalan", "last_name"=>"Liv", "scopus_author_id"=>"55587215900"}, {"first_name"=>"A. Christiaan", "last_name"=>"Zonnevylle", "scopus_author_id"=>"25931281400"}, {"first_name"=>"Angela C.", "last_name"=>"Narvaez", "scopus_author_id"=>"35196596600"}, {"first_name"=>"Andries P.J.", "last_name"=>"Effting", "scopus_author_id"=>"55587319100"}, {"first_name"=>"Philip W.", "last_name"=>"Voorneveld", "scopus_author_id"=>"53980726400"}, {"first_name"=>"Miriam S.", "last_name"=>"Lucas", "scopus_author_id"=>"37041895000"}, {"first_name"=>"James C.", "last_name"=>"Hardwick", "scopus_author_id"=>"35477624800"}, {"first_name"=>"Roger A.", "last_name"=>"Wepf", "scopus_author_id"=>"7003583346"}, {"first_name"=>"Pieter", "last_name"=>"Kruit", "scopus_author_id"=>"55409441300"}, {"first_name"=>"Jacob P.", "last_name"=>"Hoogenboom", "scopus_author_id"=>"7004242619"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"368325914", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0055707", "scopus"=>"2-s2.0-84873694839", "pmid"=>"23409024", "sgr"=>"84873694839"}, "id"=>"d174274d-cea5-348c-8756-57f246ba7789", "abstract"=>"Correlative light and electron microscopy (CLEM) is a unique method for investigating biological structure-function relations. With CLEM protein distributions visualized in fluorescence can be mapped onto the cellular ultrastructure measured with electron microscopy. Widespread application of correlative microscopy is hampered by elaborate experimental procedures related foremost to retrieving regions of interest in both modalities and/or compromises in integrated approaches. We present a novel approach to correlative microscopy, in which a high numerical aperture epi-fluorescence microscope and a scanning electron microscope illuminate the same area of a sample at the same time. This removes the need for retrieval of regions of interest leading to a drastic reduction of inspection times and the possibility for quantitative investigations of large areas and datasets with correlative microscopy. We demonstrate Simultaneous CLEM (SCLEM) analyzing cell-cell connections and membrane protrusions in whole uncoated colon adenocarcinoma cell line cells stained for actin and cortactin with AlexaFluor488. SCLEM imaging of coverglass-mounted tissue sections with both electron-dense and fluorescence staining is also shown.", "link"=>"http://www.mendeley.com/research/simultaneous-correlative-scanning-electron-highna-fluorescence-microscopy", "reader_count"=>71, "reader_count_by_academic_status"=>{"Unspecified"=>3, "Professor > Associate Professor"=>4, "Researcher"=>16, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>25, "Student > Postgraduate"=>2, "Other"=>7, "Student > Master"=>8, "Student > Bachelor"=>2, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>3, "Professor > Associate Professor"=>4, "Researcher"=>16, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>25, "Student > Postgraduate"=>2, "Other"=>7, "Student > Master"=>8, "Student > Bachelor"=>2, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>4, "Agricultural and Biological Sciences"=>28, "Chemistry"=>6, "Computer Science"=>2, "Engineering"=>4, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>4, "Materials Science"=>2, "Medicine and Dentistry"=>4, "Neuroscience"=>1, "Physics and Astronomy"=>13, "Social Sciences"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Materials Science"=>{"Materials Science"=>2}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Social Sciences"=>{"Social Sciences"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>13}, "Unspecified"=>{"Unspecified"=>4}, "Environmental Science"=>{"Environmental Science"=>1}, "Engineering"=>{"Engineering"=>4}, "Chemistry"=>{"Chemistry"=>6}, "Neuroscience"=>{"Neuroscience"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>28}, "Computer Science"=>{"Computer Science"=>2}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}}, "reader_count_by_country"=>{"Netherlands"=>3, "France"=>1, "Portugal"=>1, "Spain"=>1}, "group_count"=>3}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84873694839"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84873694839?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84873694839&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84873694839&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84873694839", "dc:identifier"=>"SCOPUS_ID:84873694839", "eid"=>"2-s2.0-84873694839", "dc:title"=>"Simultaneous Correlative Scanning Electron and High-NA Fluorescence Microscopy", "dc:creator"=>"Liv N.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"8", "prism:issueIdentifier"=>"2", "prism:pageRange"=>nil, "prism:coverDate"=>"2013-02-08", "prism:coverDisplayDate"=>"8 February 2013", "prism:doi"=>"10.1371/journal.pone.0055707", "citedby-count"=>"50", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Delft University of Technology", "affiliation-city"=>"Delft", "affiliation-country"=>"Netherlands"}], "pubmed-id"=>"23409024", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e55707", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0055707", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"64", "xml_views"=>"3", "html_views"=>"374"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"28", "xml_views"=>"0", "html_views"=>"117"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"30", "xml_views"=>"3", "html_views"=>"109"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"25", "xml_views"=>"1", "html_views"=>"91"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"91"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"26", "xml_views"=>"2", "html_views"=>"128"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"32", "xml_views"=>"0", "html_views"=>"125"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"33", "xml_views"=>"0", "html_views"=>"99"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"2", "html_views"=>"104"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"103"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"4", "html_views"=>"53"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"64"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"83"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"95"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"62"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"56"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"69"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"108"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"18", "xml_views"=>"1", "html_views"=>"113"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"40"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"72"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"91"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"2", "html_views"=>"42"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"2", "html_views"=>"57"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"35"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"2", "html_views"=>"37"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"49"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"15"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"5", "html_views"=>"10"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"7", "html_views"=>"11"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"6", "html_views"=>"7"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"5", "html_views"=>"10"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"7"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/491387"], "description"=>"<p>(a) schematic lay-out for SCLEM, BSE: backscattered electrons, SE: secondary electrons, ETD: Everhard-Thornley detector, LED: light emitting diode, CCD: charge coupled device camera. (b) inside view of the integrated microscope for SCLEM showing optical objective lens in epi-configuration underneath sample holder and electron lens.</p>", "links"=>[], "tags"=>["correlative", "electron"], "article_id"=>161878, "categories"=>["Cancer", "Cell Biology", "Physics", "Biophysics", "Immunology"], "users"=>["Nalan Liv", "A. Christiaan Zonnevylle", "Angela C. Narvaez", "Andries P. J. Effting", "Philip W. Voorneveld", "Miriam S. Lucas", "James C. Hardwick", "Roger A. Wepf", "Pieter Kruit", "Jacob P. Hoogenboom"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0055707.g001", "stats"=>{"downloads"=>0, "page_views"=>27, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Simultaneous_Correlative_Light_and_Electron_Microscopy_/161878", "title"=>"Simultaneous Correlative Light and Electron Microscopy.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 15:36:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/492115"], "description"=>"<p>(a) FM image of human skin tissue stained with DiIC18 fluorescence and uranyl acetate and osmium tetroxide for EM contrast. Scalebar 5 µm (b) BSE image of a selected region from (a), showing a cell nucleus not discernible in (a) (marked with a red arrow), and bundles of longitudinally and transversally cut collagen fibers. Scalebar is 5 µm (c, d) High-magnification images of the areas marked with (c) a red star, scalebar 1 µm, and (d) a yellow star, scalebar 2 µm.</p>", "links"=>[], "tags"=>["fm", "em", "stained"], "article_id"=>162622, "categories"=>["Cancer", "Cell Biology", "Physics", "Biophysics", "Immunology"], "users"=>["Nalan Liv", "A. Christiaan Zonnevylle", "Angela C. Narvaez", "Andries P. J. Effting", "Philip W. Voorneveld", "Miriam S. Lucas", "James C. Hardwick", "Roger A. Wepf", "Pieter Kruit", "Jacob P. Hoogenboom"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0055707.g004", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_SCLEM_on_FM_and_EM_stained_tissue_sections_/162622", "title"=>"SCLEM on FM and EM stained tissue sections.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 15:40:33"}
  • {"files"=>["https://ndownloader.figshare.com/files/491596"], "description"=>"<p>(a) FM image of three adenocarcinoma cells actin labeled with Alexa488. The three cells are connected via tentacles and larger extrusions. Scalebar 5 µm. (b) SEM image of the boxed area in (a), showing detailed information on the connections between the cells. A dense network of tentacles and lamellae stretches between the upper and the right cell. Scalebar 3 µm(c) FM image of an extension connecting another two adenocarcinoma cells. Clear variations in actin concentration along the extrusion can be observed. (b) BSE image of the extrusion in (a). Red arrows mark areas with increased concentration of tentacles that occur before and after the thinner parts of the extrusion. Scale bar is 10 µm. (d, e) SE and BSE high-magnification images of the boxed areas in (b) showing a region rich in tentacles and small lamellar extrusions. Scale bars are 2 µm. (f) Fluorescence intensity profiles, normalized on the maximum, taken along the red and blue lines in (a). (g) Normalized SE intensity profile taken at the corresponding locations marked in (c).</p>", "links"=>[], "tags"=>["uncoated"], "article_id"=>162106, "categories"=>["Cancer", "Cell Biology", "Physics", "Biophysics", "Immunology"], "users"=>["Nalan Liv", "A. Christiaan Zonnevylle", "Angela C. Narvaez", "Andries P. J. Effting", "Philip W. Voorneveld", "Miriam S. Lucas", "James C. Hardwick", "Roger A. Wepf", "Pieter Kruit", "Jacob P. Hoogenboom"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0055707.g002", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_SCLEM_of_whole_uncoated_cells_/162106", "title"=>"SCLEM of whole uncoated cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 15:37:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/491865"], "description"=>"<p>(a) After mounting the sample and vacuum pumping of the SEM chamber, a low-magnification image in SEM mode is taken to inspect surface coverage and position the sample stage. (b) An isolated cell is identified and the SEM focus is fine-tuned for high-magnification imaging (c) The fluorescence image is recorded after the marked cell was selected. Based on the spatial variations in cortactin distribution and the structural overview in (b), regions of interest are identified for high-magnification imaging in SEM mode. Blue, red, and yellow arrows indicate different type of regions with a local increase in cortactin density. Corresponding areas are also marked in the SEM images. (d) SE image recorded at 20keV of the region of interest identified in (c). The cortactin-rich areas marked with red arrows are directly neighboring regions with larger extrusions and high density of tentacles (e) BSE image recorded at 5keV. (f) SE image at 5keV reveals the details in surface topography. It can be clearly seen that the blue marked cortactin-rich regions located in the cell interior correspond to an increase in cell thickness. The cortactin-rich regions marked with yellow arrows surround a larger thin lamellar outgrowth with numerous extending tentacles. Typical time involved in such a procedure (sample mounting & pump down – a,b – c – d,e,f) amounts to 20–35 minutes (4 min –5 min –5 min –5–15 min).</p>", "links"=>[], "tags"=>["cortactin", "labeled", "adenocancerinoma"], "article_id"=>162375, "categories"=>["Cancer", "Cell Biology", "Physics", "Biophysics", "Immunology"], "users"=>["Nalan Liv", "A. Christiaan Zonnevylle", "Angela C. Narvaez", "Andries P. J. Effting", "Philip W. Voorneveld", "Miriam S. Lucas", "James C. Hardwick", "Roger A. Wepf", "Pieter Kruit", "Jacob P. Hoogenboom"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0055707.g003", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_SCLEM_inspection_procedure_with_Cortactin_labeled_adenocancerinoma_cells_/162375", "title"=>"SCLEM inspection procedure with Cortactin labeled adenocancerinoma cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-19 15:39:10"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"31", "full-text"=>"26", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"28", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"26", "full-text"=>"29", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"21", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"26", "full-text"=>"26", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"13", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"18", "full-text"=>"14", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"2", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"5", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"13", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"16", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"22", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"19", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"20", "full-text"=>"19", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"26", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"18", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"18", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}

Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Engineering and technology", "average_usage"=>[254, 440, 558, 675, 785, 883, 972, 1061, 1154, 1248, 1336, 1414, 1489]}, {"subject_area"=>"/Engineering and technology/Equipment", "average_usage"=>[246, 440, 558, 647, 746, 842, 937, 1028, 1116, 1191, 1253, 1348, 1420]}]}
Loading … Spinner
There are currently no alerts