High Prevalence of Mucosa-Associated E. coli Producing Cyclomodulin and Genotoxin in Colon Cancer
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{"title"=>"High Prevalence of Mucosa-Associated E. coli Producing Cyclomodulin and Genotoxin in Colon Cancer", "type"=>"journal", "authors"=>[{"first_name"=>"Emmanuel", "last_name"=>"Buc", "scopus_author_id"=>"6506498905"}, {"first_name"=>"Damien", "last_name"=>"Dubois", "scopus_author_id"=>"25122340800"}, {"first_name"=>"Pierre", "last_name"=>"Sauvanet", "scopus_author_id"=>"24588066300"}, {"first_name"=>"Jennifer", "last_name"=>"Raisch", "scopus_author_id"=>"55599517600"}, {"first_name"=>"Julien", "last_name"=>"Delmas", "scopus_author_id"=>"56216503300"}, {"first_name"=>"Arlette", "last_name"=>"Darfeuille-Michaud", "scopus_author_id"=>"35407859900"}, {"first_name"=>"Denis", "last_name"=>"Pezet", "scopus_author_id"=>"7006170778"}, {"first_name"=>"Richard", "last_name"=>"Bonnet", "scopus_author_id"=>"7005875463"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84874007506", "doi"=>"10.1371/journal.pone.0056964", "pui"=>"368353872", "pmid"=>"23457644", "scopus"=>"2-s2.0-84874007506", "issn"=>"19326203"}, "id"=>"2337ded0-85f6-3f1a-918f-b844c05cc6c1", "abstract"=>"Some Escherichia coli strains produce toxins designated cyclomodulins (CMs) which interfere with the eukaryotic cell cycle of host cells, suggesting a possible link between these bacteria and cancers. There are relatively few data available concerning the colonization of colon tumors by cyclomodulin- and genotoxic-producing E. coli. We did a qualitative and phylogenetic analysis of mucosa-associated E. coli harboring cyclomodulin-encoding genes from 38 patients with colorectal cancer (CRC) and 31 with diverticulosis. The functionality of these genes was investigated on cell cultures and the genotoxic activity of strains devoid of known CM-encoding gene was investigated. Results showed a higher prevalence of B2 phylogroup E. coli harboring the colibatin-producing genes in biopsies of patients with CRC (55.3%) than in those of patients with diverticulosis (19.3%), (p<0.01). Likewise, a higher prevalence of B2 E. coli harboring the CNF1-encoding genes in biopsies of patients with CRC (39.5%) than in those of patients with diverticulosis (12.9%), (p = 0.01). Functional analysis revealed that the majority of these genes were functional. Analysis of the ability of E. coli to adhere to intestinal epithelial cells Int-407 indicated that highly adherent E. coli strains mostly belonged to A and D phylogroups, whatever the origin of the strains (CRC or diverticulosis), and that most E. coli strains belonging to B2 phylogroup displayed very low levels of adhesion. In addition, 27.6% (n = 21/76) E. coli strains devoid of known cyclomodulin-encoding genes induced DNA damage in vitro, as assessed by the comet assay. In contrast to cyclomodulin-producing E. coli, these strains mainly belonged to A or D E. coli phylogroups, and exhibited a non significant difference in the distribution of CRC and diverticulosis specimens (22% versus 32.5%, p = 0.91). In conclusion, cyclomodulin-producing E. coli belonging mostly to B2 phylogroup colonize the colonic mucosa of patients with CRC.", "link"=>"http://www.mendeley.com/research/high-prevalence-mucosaassociated-e-coli-producing-cyclomodulin-genotoxin-colon-cancer", "reader_count"=>102, "reader_count_by_academic_status"=>{"Unspecified"=>3, "Professor > Associate Professor"=>5, "Researcher"=>21, "Student > Doctoral Student"=>12, "Student > Ph. D. Student"=>20, "Student > Postgraduate"=>7, "Student > Master"=>17, "Other"=>3, "Student > Bachelor"=>10, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>2, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>3, "Professor > Associate Professor"=>5, "Researcher"=>21, "Student > Doctoral Student"=>12, "Student > Ph. D. Student"=>20, "Student > Postgraduate"=>7, "Student > Master"=>17, "Other"=>3, "Student > Bachelor"=>10, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>2, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>6, "Engineering"=>1, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>21, "Agricultural and Biological Sciences"=>38, "Medicine and Dentistry"=>12, "Veterinary Science and Veterinary Medicine"=>3, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Chemistry"=>4, "Computer Science"=>1, "Immunology and Microbiology"=>14}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>12}, "Chemistry"=>{"Chemistry"=>4}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>14}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>38}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>21}, "Unspecified"=>{"Unspecified"=>6}, "Environmental Science"=>{"Environmental Science"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>3}}, "reader_count_by_country"=>{"Hong Kong"=>1, "Japan"=>1, "United Kingdom"=>1, "France"=>1, "Chile"=>1, "Germany"=>1}, "group_count"=>4}

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Figshare

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  • {"files"=>["https://ndownloader.figshare.com/files/489175"], "description"=>"<p>Number of <i>E. coli</i> strains collected from patients with CRC and diverticulosis.</p>", "links"=>[], "tags"=>["strains", "patients", "crc"], "article_id"=>159689, "categories"=>["Infectious Diseases", "Microbiology", "Chemistry", "Cancer", "Biotechnology"], "users"=>["Emmanuel Buc", "Damien Dubois", "Pierre Sauvanet", "Jennifer Raisch", "Julien Delmas", "Arlette Darfeuille-Michaud", "Denis Pezet", "Richard Bonnet"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0056964.t001", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Number_of_E_coli_strains_collected_from_patients_with_CRC_and_diverticulosis_/159689", "title"=>"Number of <i>E. coli</i> strains collected from patients with CRC and diverticulosis.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-14 02:41:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/488973"], "description"=>"<p>DNA damage was not detected in HeLa cells infected with the <i>E. coli</i> strain IHE3034 Δ<i>clbP</i> harboring a defective <i>pks</i> island (A). Comet assay was positive in HeLa cells infected with the <i>E. coli</i> strain IHE3034 harboring <i>pks</i> island (B) or with <i>E. coli</i> devoid of known cyclomodulin-encoding genes (C).</p>", "links"=>[], "tags"=>["was", "comet", "assay", "hela", "cells", "exposed"], "article_id"=>159485, "categories"=>["Infectious Diseases", "Microbiology", "Chemistry", "Cancer", "Biotechnology"], "users"=>["Emmanuel Buc", "Damien Dubois", "Pierre Sauvanet", "Jennifer Raisch", "Julien Delmas", "Arlette Darfeuille-Michaud", "Denis Pezet", "Richard Bonnet"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0056964.g003", "stats"=>{"downloads"=>4, "page_views"=>35, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_DNA_damage_was_detected_by_the_comet_assay_in_HeLa_cells_exposed_to_E_coli_for_3_h_/159485", "title"=>"DNA damage was detected by the comet assay in HeLa cells exposed to <i>E. coli</i> for 3 h.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-14 02:38:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/486010", "https://ndownloader.figshare.com/files/486015", "https://ndownloader.figshare.com/files/486018", "https://ndownloader.figshare.com/files/486021", "https://ndownloader.figshare.com/files/486024"], "description"=>"<div><p>Some <em>Escherichia coli</em> strains produce toxins designated cyclomodulins (CMs) which interfere with the eukaryotic cell cycle of host cells, suggesting a possible link between these bacteria and cancers. There are relatively few data available concerning the colonization of colon tumors by cyclomodulin- and genotoxic-producing <em>E. coli</em>. We did a qualitative and phylogenetic analysis of mucosa-associated <em>E. coli</em> harboring cyclomodulin-encoding genes from 38 patients with colorectal cancer (CRC) and 31 with diverticulosis. The functionality of these genes was investigated on cell cultures and the genotoxic activity of strains devoid of known CM-encoding gene was investigated. Results showed a higher prevalence of B2 phylogroup <em>E. coli</em> harboring the colibatin-producing genes in biopsies of patients with CRC (55.3%) than in those of patients with diverticulosis (19.3%), (p<0.01). Likewise, a higher prevalence of B2 <em>E. coli</em> harboring the CNF1-encoding genes in biopsies of patients with CRC (39.5%) than in those of patients with diverticulosis (12.9%), (p = 0.01). Functional analysis revealed that the majority of these genes were functional. Analysis of the ability of <em>E. coli</em> to adhere to intestinal epithelial cells Int-407 indicated that highly adherent <em>E. coli</em> strains mostly belonged to A and D phylogroups, whatever the origin of the strains (CRC or diverticulosis), and that most <em>E. coli</em> strains belonging to B2 phylogroup displayed very low levels of adhesion. In addition, 27.6% (n = 21/76) <em>E. coli</em> strains devoid of known cyclomodulin-encoding genes induced DNA damage <em>in vitro</em>, as assessed by the comet assay. In contrast to cyclomodulin-producing <em>E. coli</em>, these strains mainly belonged to A or D <em>E. coli</em> phylogroups, and exhibited a non significant difference in the distribution of CRC and diverticulosis specimens (22% <em>versus</em> 32.5%, p = 0.91). In conclusion, cyclomodulin-producing <em>E. coli</em> belonging mostly to B2 phylogroup colonize the colonic mucosa of patients with CRC.</p> </div>", "links"=>[], "tags"=>["prevalence", "mucosa-associated", "producing", "cyclomodulin", "genotoxin", "colon", "cancer"], "article_id"=>157064, "categories"=>["Infectious Diseases", "Microbiology", "Chemistry", "Cancer", "Biotechnology"], "users"=>["Emmanuel Buc", "Damien Dubois", "Pierre Sauvanet", "Jennifer Raisch", "Julien Delmas", "Arlette Darfeuille-Michaud", "Denis Pezet", "Richard Bonnet"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0056964.s001", "https://dx.doi.org/10.1371/journal.pone.0056964.s002", "https://dx.doi.org/10.1371/journal.pone.0056964.s003", "https://dx.doi.org/10.1371/journal.pone.0056964.s004", "https://dx.doi.org/10.1371/journal.pone.0056964.s005"], "stats"=>{"downloads"=>20, "page_views"=>48, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/High_Prevalence_of_Mucosa_Associated_E_coli_Producing_Cyclomodulin_and_Genotoxin_in_Colon_Cancer__/157064", "title"=>"High Prevalence of Mucosa-Associated <em>E. coli</em> Producing Cyclomodulin and Genotoxin in Colon Cancer", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-02-14 01:57:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/489218"], "description"=>"<p>Colonization of diverticulosis and CRC samples by <i>E. coli</i> phylogroups (A, B1, B2 and D).</p>", "links"=>[], "tags"=>["diverticulosis", "crc", "samples", "phylogroups", "b2"], "article_id"=>159727, "categories"=>["Infectious Diseases", "Microbiology", "Chemistry", "Cancer", "Biotechnology"], "users"=>["Emmanuel Buc", "Damien Dubois", "Pierre Sauvanet", "Jennifer Raisch", "Julien Delmas", "Arlette Darfeuille-Michaud", "Denis Pezet", "Richard Bonnet"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0056964.t002", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Colonization_of_diverticulosis_and_CRC_samples_by_E_coli_phylogroups_A_B1_B2_and_D_/159727", "title"=>"Colonization of diverticulosis and CRC samples by <i>E. coli</i> phylogroups (A, B1, B2 and D).", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-14 02:42:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/488893"], "description"=>"<p>A, <i>E. coli</i> strains (n = 70) isolated from CRC samples (n = 38), and B, <i>E. coli</i> strains (n = 46) isolated from diverticulosis samples (n = 31).</p>", "links"=>[], "tags"=>["strains", "producing", "cyclomodulins", "phylogroups", "specimen"], "article_id"=>159408, "categories"=>["Infectious Diseases", "Microbiology", "Chemistry", "Cancer", "Biotechnology"], "users"=>["Emmanuel Buc", "Damien Dubois", "Pierre Sauvanet", "Jennifer Raisch", "Julien Delmas", "Arlette Darfeuille-Michaud", "Denis Pezet", "Richard Bonnet"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0056964.g002", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Distribution_of_E_coli_strains_producing_various_cyclomodulins_according_to_phylogroups_and_specimen_origins_/159408", "title"=>"Distribution of <i>E. coli</i> strains producing various cyclomodulins according to phylogroups and specimen origins.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-14 02:36:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/489141"], "description"=>"1<p>, cyclomodulin-encoding gene;</p>2<p>, some <i>E. coli</i> strains harbored more than one CM-encoding genes.</p>", "links"=>[], "tags"=>["crc", "diverticulosis"], "article_id"=>159656, "categories"=>["Infectious Diseases", "Microbiology", "Chemistry", "Cancer", "Biotechnology"], "users"=>["Emmanuel Buc", "Damien Dubois", "Pierre Sauvanet", "Jennifer Raisch", "Julien Delmas", "Arlette Darfeuille-Michaud", "Denis Pezet", "Richard Bonnet"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0056964.t004", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_CM_encoding_gene_content_of_CRC_and_diverticulosis_samples_/159656", "title"=>"CM-encoding gene content of CRC and diverticulosis samples.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-14 02:40:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/488777"], "description"=>"<p>For CNF and CDT, the cytopathic effect is only observable with bacterial lysates. In contrast, for colibactin and CIF, a contact between bacteria and host cells is required. Colibactin, CDT and CIF induced cytopathic effects as seen by enlarged nuclei and cell distension (megalocytosis), while CNF induced multinucleation and enlargement of HeLa cells.</p>", "links"=>[], "tags"=>["induced", "extracts", "epithelial", "cells", "three-day"], "article_id"=>159288, "categories"=>["Infectious Diseases", "Microbiology", "Chemistry", "Cancer", "Biotechnology"], "users"=>["Emmanuel Buc", "Damien Dubois", "Pierre Sauvanet", "Jennifer Raisch", "Julien Delmas", "Arlette Darfeuille-Michaud", "Denis Pezet", "Richard Bonnet"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0056964.g001", "stats"=>{"downloads"=>5, "page_views"=>95, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cytopathic_effect_induced_by_E_coli_live_bacteria_or_protein_extracts_on_epithelial_cells_at_three_day_post_infection_/159288", "title"=>"Cytopathic effect induced by <i>E. coli</i> live bacteria or protein extracts on epithelial cells at three-day post-infection.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-14 02:34:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/489061"], "description"=>"<p>A, adhesion ability according to the specimen origin and E. coli phylogroup. B, Adhesion ability of <i>E. coli</i> strains isolated from diverticulosis and CRC (proximal and distal) samples and belonging to A and D phylogroups according to their ability to produce or not a cyclomodulin/genotoxin. The results are expressed as number of adherent bacteria per cell after 3 h infection period. Data are means+/−SEM for at least 3 independent experiments.</p>", "links"=>[], "tags"=>["strains", "diverticulosis", "crc", "samples", "int-407", "intestinal", "epithelial"], "article_id"=>159571, "categories"=>["Infectious Diseases", "Microbiology", "Chemistry", "Cancer", "Biotechnology"], "users"=>["Emmanuel Buc", "Damien Dubois", "Pierre Sauvanet", "Jennifer Raisch", "Julien Delmas", "Arlette Darfeuille-Michaud", "Denis Pezet", "Richard Bonnet"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0056964.g004", "stats"=>{"downloads"=>1, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Adhesion_ability_of_E_coli_strains_isolated_from_diverticulosis_and_CRC_proximal_and_distal_samples_to_Int_407_intestinal_epithelial_cells_/159571", "title"=>"Adhesion ability of <i>E. coli</i> strains isolated from diverticulosis and CRC (proximal and distal) samples to Int-407 intestinal epithelial cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-14 02:39:31"}

PMC Usage Stats | Further Information

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  • {"unique-ip"=>"25", "full-text"=>"26", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
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Relative Metric

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